LOCUS       79#pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        19518 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT0_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 79
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000103120
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7243..7407
                     /db_xref="ENSEMBL:ENSMUSE00000661323"
                     /rank="1"
                     /note="ENSMUSE00000661323"
     misc_feature    4275..8351
                     /note="5 arm"
     misc_feature    8379..8403
                     /note="B1 Gateway"
     misc_feature    8416..8463
                     /note="Frt"
     misc_feature    8464..8963
                     /note="mouse En2 intron"
     primer          complement(8532..8553)
                     /note="LRPCR LAR3_EN2"
     primer          complement(8428..8451)
                     /note="FRT3"
     primer          12615..12633
                     /note="seq primer NF"
     misc_feature    12675..12682
                     /note="RE sites"
     misc_feature    14475..14508
                     /note="loxP"
     misc_feature    complement(12683..12714)
                     /note="Rox"
     misc_feature    complement(14435..14466)
                     /note="Rox"
     misc_feature    12715..12736
                     /note="polylinker"
     CDS             13295..13891
                     /note="puromycin N-acetyl transferase"
     misc_feature    13410..13415
                     /note="Mutated RsrII site"
     intron          8964..9338
                     /note="mouse En2 intron"
     misc_feature    9336..9338
                     /note="Splice Acceptor"
     exon            9339..9378
                     /note="mouse En2 exon"
     primer          9239..9260
                     /note="seq primer FCHK2"
     misc_feature    9379..9501
                     /note="1Dleader"
     misc_feature    9502..9553
                     /note="F2A"
     misc_feature    9649..10365
                     /note="EGFP"
     misc_feature    9559..9648
                     /note="3X NLS"
     misc_feature    12431..12669
                     /note="SV40 polyadenylation site"
     misc_feature    11464..12408
                     /note="ERT2"
     promoter        12747..13258
                     /note="PGK promoter"
     misc_feature    14002..14434
                     /note="PGK pA"
     primer          12489..12512
                     /note="LRPCR RAF5"
     misc_feature    10375..10428
                     /note="T2A"
     misc_feature    10425..10425
                     /note=" A to G to kill RsRII site"
     misc_feature    10429..11454
                     /note="Cre"
     misc_feature    9354..9379
                     /note="FCHK lf2a_lacz_gt0"
     misc_feature    9354..9369
                     /note="FCHK QC prefix region"
     misc_feature    9370..9373
                     /note="FCHK QC critical region"
     misc_feature    9374..9379
                     /note="FCHK QC suffix region"
     misc_feature    14531..14551
                     /note="B2 Gateway"
     misc_feature    8352..14581
                     /note="Synthetic Cassette"
     exon            14798..15467
                     /db_xref="ENSEMBL:ENSMUSE00000417980"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000417980"
     misc_feature    14582..15631
                     /note="Critical Region"
     misc_feature    15655..15688
                     /note="Downstream LoxP"
     misc_feature    15632..15711
                     /note="synthetic loxP region"
     exon            17469..17608
                     /db_xref="ENSEMBL:ENSMUSE00001297842"
                     /rank="3"
                     /note="ENSMUSE00001297842"
     exon            18903..19518
                     /db_xref="ENSEMBL:ENSMUSE00001222629"
                     /rank="4"
                     /note="ENSMUSE00001222629 5' fragment"
     misc_feature    15712..19518
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacattaa atatgcgcct ttaactttta tagatcttga gtgacactgt
     4321 gctgcctccc acccctcgtt ctcagtaggg accaatggaa gagatgagca gggtgggtgg
     4381 agaagcaggt gagtgtctga gagcgcagaa ccctgaaaga acaggaaagg aaagccccga
     4441 gaaccgttga ctcagtttcc ccaggatgta caaggcttgg gagtggggtg gagatataca
     4501 gaaaccccag ctctgctgac atcatccatc ttataaagcc tgtaggcctt cagagtagat
     4561 tggagtaaaa gctgtgtcta ccggcagact gtctgggaga agagtcccag aatgtgcggc
     4621 ctcctctgtg cagccttaag ctgccctggg aatattatgt tctagaacag cacagaaatc
     4681 ccactgagaa ggctgtgact tcctagagtt agcgaataaa catctggcct caaaaggatc
     4741 agcctgggct ggagagatgg ctcagtggtt aacagcagtc ctgagttcaa ttcccagcaa
     4801 ccatgtggtg gctcacaact gtctgtaatg ggatctgatg ccctcttcta gtggatctga
     4861 aaacagcaac agtgttctca tacacataaa ataaataaat aaatctggaa aaaaaaaaga
     4921 tcaggccatc ttagggtttt aactactgtg aacagacacc gtgaccaagg caactcttag
     4981 acaacattta attggggatg gtggtttaca ggttcagacg attattatta aggtgggatt
     5041 gtggcagaat ccaggcaggc tggaggacct gagagttcta tatcttcatc tgaaggctgc
     5101 tatgagaaga ctgacttcca ggaagctaga atgagggtct taaagcccat gtccacagtg
     5161 acacactttc tccaacagag tcacacctcc taatggtgcc acaccctggg ccaagcatat
     5221 acaaaccatc atgcaaggcc tttgccatgt ggtccagaag cagggataaa gatgacttag
     5281 gctggatgaa gccaggattc cagtcaagtg agaggcaatt caaaggcaga atgactttgg
     5341 tccccaaact tatccccagg tcaagtccag gaacttgtgc ctactagaca agtcttctaa
     5401 caccaagcta tagtcccagt ccctagataa aagtatttta aatcatgtct ttttaaaatt
     5461 acattttaaa tgaggccgga gagatagcgc agaggctaag agcaagggct gctctttaag
     5521 aggacctgag ttcaattccc agcaaccaca tggcagctca cacctgtcta taactctagt
     5581 tccagaaggt tctaaacccc cacatagaca catatgcagg cgaaacacca atacacataa
     5641 aataaaactc atctattaag ccaggcagtg gtggtacaca ttgttagtcc cagcatcagg
     5701 gaggcagagg cagccggatc tctgtgagtt tgaggccagc ctggtctaca cagtgaattc
     5761 caggatagcc agagctggac agagagatct ctcaaagaaa taaaaagtac attttaattt
     5821 atttttgtgt gcatttatgt gtgcggacct acacacctac tacctgcctg tggctgtcag
     5881 agaagaacaa gtcgagggag ttggttttct atcattcatg gcctagaagt tcaactcagg
     5941 gtaacgtggc catgttaaca aagctatatc tcaactgctc ctgagaaatt atatataatt
     6001 atatatccgt ttgttttgtt ttgttttgtt gagacagggt gtctctctgt aggcctggct
     6061 gtcctggaac tcactctgca gatcaggctg gcctggaact cactgagatc tgcttgcctc
     6121 tgcctcccaa gtgctgggat taaagaaaaa agtgaaagtg aacaatgtca ccacgtcctc
     6181 gaagctagga tgcacttgcc tagcttgcag gaaccccagc atcttaaaca gctgacatgt
     6241 tcttgtatgt atgaatttcc agcaagagga taagaagttc aaggtcaccc tttgttacat
     6301 agagagtttg aacttagcct gggttacatg agattcatct ttaaaacaaa ccttttttgg
     6361 gggatatcca gcaaagaaga ctgcttagac ccaagcggaa gccaatagaa agtctttatt
     6421 agccagcccc tcagcccctt cactagactt tttttttttt tttttttttt ttcctgagac
     6481 agggtttctc tgtgtagacc tggctgtcct ggaactcacc ctgtagacca ggctggcctt
     6541 gaactcagaa atccacctgc ctctgccttc caagtgctgg gattaaaggt gtgtgccacc
     6601 accacccagc acttttctca gggtaggctt ttaatcataa aaaccacatc ctgtgttgac
     6661 acacctaagt cagaagcaga ctataggagc caaaaatcaa ggttaggaca tttagggcct
     6721 ttcccagaat tatggacttt gatgaattag gtctttgttt ttgttttagg aggcggtgat
     6781 gcctatcggc ctggtttcgt ggcctgaatg gtacttcctt cacatgtcag ttgtgataag
     6841 gtctatgagc ctgtttcaaa attttaaact atctgactgc acccatttca tcccagcctg
     6901 cctgggaacc caggaaaggc aactgcctcc tgttttaaga gttcatctct ctcctggtga
     6961 tgtctttaat ccaagcactt gggaggcaga ggcaggcaga tctctgtacc aggccagcct
     7021 gatctacatg gtgagttcca ggacagtcag ggctacatag tgagttcctg tctcagagaa
     7081 gggaaaaagt taaactttaa cttttaagga acaaagaggt ggcgtggcgg actctttctt
     7141 ggcattgaga gtccaggtgt gcttttactt tctgccaagg ctcagaccgc tgcttcctgg
     7201 cactcaggtt tccattcccc gggggcgcgt gcgcagaggc tcgggctgac cccgccctgt
     7261 ccgcgcgccc cgcccccagg ctatgaaagg gaccctgcgt gggggggccc cggacacccg
     7321 gagacgcccg tgtccctcac gcaggcgggc ggccccggag acatagtacc cgcaaaggcg
     7381 gtgacggcgg tgcgcccact catcatggtg agcgcagcgc gtgcgtgggg gccggggcgg
     7441 gagagaggct aactgaaagc gagattcttg gggatgcatg ggcagaagta gtttgcaaat
     7501 gaggacacca ggttccggag ttgaagttgc ctctgggtcc tccaaggcag ccctaaggca
     7561 aggggcgcga gcggggatat tttgggggag tcttagtgtc tttggagaag gaaatatcgc
     7621 cgcagggaag tgaaagtcga tgctccttgg cgacaccagc tggcaaatgg gtctgggtag
     7681 caccagagag tgcagggctg ttgtggagcc cgcgctggga gtgggttcct gcacaaggct
     7741 atagagaagc cagaagccag ctaaattctc aggggcggaa gccgttgtag atgacaactg
     7801 aaaccgctat gtcttgtttt tgtcagagag gtgctgctaa cacacagcag aatagggggc
     7861 acagatctct gaatacaggt gccctgtgcc ctaatcctac agattggggg agggggcgca
     7921 ctgagagagc agaggaggag gtggttgtga atatgccctt tgtccagtgc cctcctgccc
     7981 tgccccccac cagccctctg tgggaccatt gtccccctat acccagacaa gagagtatta
     8041 tctgttggtg ccattgttgg gtggagggag gcggtctttg ggtcaccctc ccctgccctc
     8101 accctcctct gcacactcaa gagagccaag gcagctgtgg ctttgcccat acatagagcc
     8161 ccaggcctcc aaacaggaca tccttaagag gtgccaagaa tatataagtt agggctgaca
     8221 gggagccaca ggcacttgct gcccttgcgt gccctgtcac caaggtgttc tgagggggag
     8281 cagagagtga tccttggagc cagagactaa gaggttaggt agcttcagaa agattccctc
     8341 taccttacaa caaggcgcat aacgatacca cgatatcaac aagtttgtac aaaaaagcag
     8401 gctggcgccg gaaccgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     8461 ttcgaaccct ttcccacacc accctccaca cttgccccaa acactgccaa ctatgtagga
     8521 ggaaggggtt gggactaaca gaagaacccg ttgtggggaa gctgttggga gggtcacttt
     8581 atgttcttgc ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg
     8641 ggtagaaggt gagagggaca ggccaccaag gtcagccccc cccccctatc ccataggagc
     8701 caggtccctc tcctggacag gaagactgaa ggggagatgc cagagactca gtgaagcctg
     8761 gggtacccta ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg
     8821 ctcctcctgg gaactctact gcccttggga tccccttgta gttgtgggtt acataggaag
     8881 ggggacggga ttccccttga ctggctagcc tactcttttc ttcagtcttc tccatctcct
     8941 ctcacctgtc tctcgaccct ttccctagga tagacttgga aaaagataag gggagaaaac
     9001 aaatgcaaac gaggccagaa agattttggc tgggcattcc ttccgctagc ttttattggg
     9061 atcccctagt ttgtgatagg ccttttagct acatctgcca atccatctca ttttcacaca
     9121 cacacacacc actttccttc tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc
     9181 acccccaatg cccaacactt gtatggcctt gggcgggtca tccccccccc cacccccagt
     9241 atctgcaacc tcaagctagc ttgggtgcgt tggttgtgga taagtagcta gactccagca
     9301 accagtaacc tctgcccttt ctcctccatg acaaccaggc tcccaggtcc cgaaaaccaa
     9361 agaagaagaa cgcagatctc tagagtcacc gagttgcttt accggatgaa gagggccgaa
     9421 acatactgtc caaggccctt gctggcaatc cacccaactg aagccagaca caaacagaaa
     9481 attgtggcac cggtgaaaca gactttgaat tttgaccttc tcaagttggc gggagacgtc
     9541 gagtccaacc ccggaccggt gcatgtggac cccaagaaaa agcggaaggt ggaccctaaa
     9601 aagaaacgca aagtggatcc taagaaaaaa agaaaggtgc acgtgtccat ggtgtccaag
     9661 ggcgaggaac tgttcaccgg cgtggtgccc atcctggtgg aactggatgg cgacgtgaac
     9721 ggccacaagt tcagcgtgtc cggcgagggc gaaggcgacg ccacatacgg aaagctgacc
     9781 ctgaagttca tctgcaccac cggcaagctg cccgtgcctt ggcctaccct cgtgaccaca
     9841 ctgacctacg gcgtgcagtg cttcagcaga taccccgacc atatgaagca gcacgacttc
     9901 ttcaagagcg ccatgcccga gggctacgtg caggaaagaa ccatcttctt taaggacgac
     9961 ggcaactaca agaccagggc cgaagtgaag ttcgagggcg acaccctcgt gaacagaatc
    10021 gagctgaagg gcatcgactt caaagaggac ggcaacatcc tgggccacaa gctggagtac
    10081 aactacaaca gccacaacgt gtacatcatg gccgacaagc agaaaaacgg catcaaagtg
    10141 aacttcaaga tccggcacaa catcgaggac ggctccgtgc agctggccga ccactaccag
    10201 cagaacaccc ctatcggcga cggccctgtg ctgctgcctg acaaccacta cctgagcacc
    10261 cagagcgccc tgagcaagga ccccaacgag aagagggacc acatggtgct gctggaattc
    10321 gtgaccgccg ctggcatcac cctgggcatg gacgagctgt acaagcctgc aggcgagggc
    10381 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccgggccgtc caatttactg
    10441 accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag
    10501 aacctgatgg acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt
    10561 ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc
    10621 gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta
    10681 aaaactatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc tgggctgcca
    10741 cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt
    10801 gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt
    10861 cgttcactca tggaaaatag cgaccgctgc caggatataa ggaatctggc atttctgggg
    10921 attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc
    10981 tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc
    11041 accgcaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt
    11101 tccgtctctg gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat
    11161 ggtgttgccg cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt
    11221 gaagcaactc atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc
    11281 tggtctggac acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca
    11341 ataccggaga tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc
    11401 cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgatctcgag
    11461 ccatctgctg gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc
    11521 tctaagaaga acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg
    11581 gatgctgagc cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct
    11641 tcgatgatgg gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg
    11701 gcgaagaggg tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa
    11761 tgtgcctggc tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg
    11821 aagctactgt ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc
    11881 atggtggaga tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg
    11941 cagggagagg agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca
    12001 tttctgtcca gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac
    12061 aagatcacag acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag
    12121 caccagcggc tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa
    12181 ggcatggagc atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg
    12241 ctggaggcgg cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg
    12301 gaggagacgg accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa
    12361 aagtattaca tcacggggga ggcagagggt ttccctgcca cagcttgatg aagatctgag
    12421 ctcttaatta atgatcataa tcagccatat cacatctgta gaggttttac ttgctttaaa
    12481 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    12541 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    12601 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    12661 tcatgtctgg atccgtttaa actaacttta aataattggc attatttaaa gttacatatg
    12721 gcggccgccc taaggctcga cctcgaaatt ctaccgggta ggggaggcgc ttttcccaag
    12781 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
    12841 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
    12901 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
    12961 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
    13021 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
    13081 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
    13141 cgggctcagg ggcggggcgg gcgcccgaag gtcttccgga ggcccggcat tctgcacgct
    13201 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
    13261 catccatcta gatctcgagc agctgaagct taccatgacc gagtacaagc ccacggtgcg
    13321 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
    13381 ctaccccgcc acgcgccaca ccgtcgatcc agatcgccac atcgagcggg tcaccgagct
    13441 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
    13501 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
    13561 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
    13621 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
    13681 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
    13741 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
    13801 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
    13861 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
    13921 gaccgaaagg agcgcacgac cccatgcatc gatgatatca gatccccggg atgcagaaat
    13981 tgatgatcta ttaaacaata aagatgtcca ctaaaatgga agtttttcct gtcatacttt
    14041 gttaagaagg gtgagaacag agtacctaca ttttgaatgg aaggattgga gctacggggg
    14101 tgggggtggg gtgggattag ataaatgcnt gctctttact gaaggctctt tactattgct
    14161 ttatgataat gtttcatagt tggatatcat aatttaaaca agcaaaacca aattaagggc
    14221 cagctcattc ctcccactca tgatctatag atctatagat ctctcgtggg atcattgttt
    14281 ttctcttgat tcccactttg tggttctaag tactgtggtt tccaaatgtg tcagtttcat
    14341 agcctgaaga acgagatcag cagcctctgt tccacataca cttcattctc agtattgttt
    14401 tgccaagttc taattccatc agaagctggt cgagtaactt taaataattg gcattattta
    14461 aagttaggcg cgccataact tcgtatagca tacattatac gaagttatgt cgagatattt
    14521 aaatattaga cccagctttc ttgtacaaag tggttgatat ctctatagtc gcagtaggcg
    14581 gttgaagata ctgaaggatc tactgcgagg aagaaaatcc accattcccc tccggggaaa
    14641 gcatccacca ttcaagggtg ctttaagtta ggcttgggga ttcctcaact gacaagcggt
    14701 ccccctctgg gagaactcgg ccagagacta gggtgtggtg cttgcgggct ggctttgagg
    14761 agccggggct gacatctcct cctcccttct tacacagaac acaagcttta cccgcaaaag
    14821 ccacacattc ctgcccaagc tcttcttcag gaaaatgtca tcctcaggag caaaggagaa
    14881 gccagagctg cagttccctt tccttcaaga tgaggacacg gtggctaccc tccacgagtg
    14941 caagacgcta ttcatcctgc gcggcctgcc gggcagcggc aagtccacac tggctcggct
    15001 catcctggag aagtaccacg acggcaccaa gatggtgtcc gctgatgctt acaagatcat
    15061 ccctggctct cgggcagact tctccgaggc gtacaagcgt ctagacgagg acctggctgg
    15121 ctactgccgc cgggacatca gggttcttgt gcttgatgat accaaccacg agcgggagcg
    15181 gctggatcag ctttttgaaa tggcagacca gtatcagtac caggtggtgc tggtggagcc
    15241 caagacagcg tggcgactag actgtgccca gctcaaggag aagaaccaat ggcagctgtc
    15301 ggccgatgac ctgaagaagc tgaagcccgg gctggagaag gactttctgc cactctactt
    15361 tggctggttc ctgaccaaaa agagttctga gaccctccga aaagctggcc aggtctttct
    15421 ggaggagctg gggaatcaca aggccttcaa gaaagagctt cgacactgta ggtggatgtg
    15481 gggtgggagc agggcagcta aagccttgta ctgtttgcct gtgatggaga gccaggcagc
    15541 gcagctgctg agctcaaagg atggggttga cgggtggagg gtacaggtag cagcttctag
    15601 gactggccag aagcaaggcg tcagtggaga agagatggcg caacgcaatt aatgataact
    15661 tcgtatagca tacattatac gaagttatgg tctgagctcg ccatcagttc aagtcttgga
    15721 ggtgggaaaa aactgtaatt ctcttcgcca tatgctataa gctcctcggt ggagggcgga
    15781 gggagaccaa ggctgtcaag atcagtttta gcagcagaat atatgtttct aagcagaagg
    15841 tgaggtggaa acctaaactg caaaaagtag caaggccaag gctgtgtgac tttccagaga
    15901 atgggtcttg ggctccccca tggcatctca agctgtagag aacccagctt catgccttcc
    15961 agaatattct ctccatataa gagacatgcc cagaaaggat ataagagttg ccacctgtac
    16021 ctgggtagga gccaggagga ttctttcctg gtaagggctg ttggctccaa tgagtggata
    16081 gtctggggta gggtagagtc ctcagtggtc ccattccccc agtgtcccac aggtagttga
    16141 gtcttgggtg ttttgttttg ttttggagat aaaagtctat gtagccttcc atggcgggcc
    16201 tggaactcac ttcatccatc aggctggtct ctgacttgca gtaattctcc tgcttttacc
    16261 ttccccagta ctggagactt aggcactctc agttttagct ggcctgtaaa caaactgctt
    16321 cattctgttg ttcgactgct cagaggcaaa caggtccttt gtgcccatgt gagttttcag
    16381 aaaacttatt tctaggctcc taagactcaa acttttacaa ggaagatgtt agttgagagc
    16441 caggccactt tttgtataga ggggcaacga tcagggacag ggcttctggc cagagctgtt
    16501 ggtgctggct cgggcatgat tactgtttga tgctggcttt ggccacaaac accagaatgc
    16561 ttttctgctc agagtgtgac aggggacatc agaaacaata acaggacgtg ctcagaatag
    16621 aggacccctt gaagcattag catttgggaa ggaatggaaa agctagagac caaattcagc
    16681 caccaagctt ggcctctgtt tggaagccca gcttttgaca tcctcctggt cctcagccac
    16741 ctcgtcccac tgttcctagg acccttcctg ttccaggccc ttggcttgct ctcctcctcc
    16801 acgcatgcct tgcagggcat gaggggtcag tcaccactgg ctgaaatggt tgctgtaaat
    16861 ctcagaggcc ctgaggaatt tggggatatg gcaactggaa ctgtcacagt ggactgacaa
    16921 gtcacccacc cacgtgtccc aggtggctag taagtggcag gatgggtttc atttccccat
    16981 gtaccacctg gttccccttg cctttggctt gtatggccta gccctgtgtt acatatgcct
    17041 gagaactcaa gtagagatat aaagctctat acgctgtaac gccagtatgg tttggtttgt
    17101 tccctgtcca gcattaaagc caggacccta tgcatgctgg gcaaggatcc cagctgccct
    17161 cccccgaccc cgttgagaac tccagatctt cctgcatcta ttttcctgga gctagggtta
    17221 catgtgtatg tcaccatgct tgctttacgc agtgctagtg ccggggactc aacctggggt
    17281 gttggcgcag gccaggccag gccagcactc tgcccactga ccttcgtctc cagctagcaa
    17341 ataagcgaag cacagcagag ccacccgagg acttcttagt aatggccctg gttttggttt
    17401 gcagtctccg agataggtgt tggtaggccc agctccccac ctttgatgga cccttttctc
    17461 ttctgcagtt atttctggag atgaacccaa ggagaagctt gagctggtca gctactttgg
    17521 aaagagacct ccaggtgtgc tgcactgtac aaccaaattc tgtgactacg ggaaggccgc
    17581 tggggcagaa gaatacgccc agcaggaggt gagtcctcct ccccagggac gcccaggtgg
    17641 aggtggggag ggtaagtggg ttggagaagt gtggcctcca gtctttggtg tagtctgtaa
    17701 tcttgttatc ctctcctagg gcctcactag tcccatctct ccccagggcc tgccaaaggg
    17761 ccttctcctt gacatagatt tatggattta tgccaggcag cagcaggttc ttgctctggc
    17821 ttctcaaacc cctgttaagt ttcagagtct caaatgctga gagtcctgta tcccagggag
    17881 cctgttctac tggccttgct ccagagggag tggctcagga taccaagcca cccattcctg
    17941 caccatagca cacagcccat ggccagatac aggcatgctg tctacttcct aacttccttt
    18001 accatggcct tccccgcagc cattcacctg cttgcttact ggccaacctc aggggaagcg
    18061 gcagctaagg ggccttgtct gttcgtgttc tcagtgtaat ggctgaatat gtaccgtcgc
    18121 tccttcctac tgtcctactt cctactgtcc gctcactaag cacctgccac atgctgctgc
    18181 tggaacagaa gttcatgaac aaaaagattt tacagttagg tcacatgaac tcacggaaga
    18241 ggctcagggt aggcagaggt aaccagatgt agaccccagc gtgttggtgg agggagccgt
    18301 ggagcaacag tagttcttca cctaccactc tgtcaatcct gtttgcttgg agggaggctc
    18361 tagtgttctc tctctcttcc aagggaacag ccaaggcaga gaggctagca gtaaacgggg
    18421 tttggatctg gtgcctctct gagagcgttt tttctaacct tagcttgaat ggcttgtgct
    18481 gtgtgttttg tttggtttgc ctttttaaaa aagacccctg caaagctggg ccgtggtggc
    18541 gcacaccttt taccccagca ctctggaggc agaggcagga ggatctcttg agttagaagc
    18601 cagcttggtc tactgagttc caggacagcc tgggctacag agaggcatgg tggcgcacgc
    18661 ctttagtccc agcacttggg aggcagaggc aggcggattt ctgagttcga ggccagcctg
    18721 gtctacagag tgagttccag gacagccagg gctacacaga gaaaccttgt ctcaaaaaac
    18781 caaaaaaacc caacacaaca caaaaacaag acccccctca caccacactc attttctccc
    18841 ctggggatgg accactctca ttagctttgt ccctctaacg tgggccccct ctccttccct
    18901 aggtggtgaa gagatcgtat ggcaaggcct tcaaactgtc catctctgct ctctttgtga
    18961 cacccaagac agctggggcc caggtggtgc tgaccgatca ggagctgcag ttgtggccca
    19021 gtgatctgga caagccatct gcctccgagg gcctgccccc agggagccga gctcacgtca
    19081 ccctaggctg tgcggccgac gtgcagccag tgcagacggg tcttgacctc ttagatattt
    19141 tacaacaggt gaaggggggc agccaaggtg aggcggtggg tgagctcccc cggggcaagc
    19201 tctattccct gggcaaaggg cgatggatgc tgagcctgac taagaagatg gaggtcaagg
    19261 ccatcttcac ggggtactat gggaagggca aacctgtgcc catacatggc agccggaagg
    19321 ggggtgccat gcagatctgc accatcatct gagggttcac accgctgtac cactgtacca
    19381 ctgctgtggg tggacacagc aacatgcctt ccacctgatt tttaagattt tttttttttt
    19441 tactcaaagc taacgtacct gtaacttttt aggagtctat aaaataacta ccagttcctt
    19501 gtgcccaccc tttctttt
//