LOCUS       272131#pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro#L3L4_pD223_DTA_T_spec        21264 bp    dna     circular UNK 
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: pL1L2_GT1_LF2A_nEGFPO_T2A_CreERT_puro
COMMENT     design_id: 272131
COMMENT     backbone: L3L4_pD223_DTA_T_spec
COMMENT     transcript_id: ENSMUST00000031650
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6690..7515
                     /db_xref="ENSEMBL:ENSMUSE00000390714"
                     /rank="1"
                     /note="ENSMUSE00000390714"
     misc_feature    4275..10249
                     /note="5 arm"
     misc_feature    10277..10301
                     /note="B1 Gateway"
     misc_feature    10314..10361
                     /note="Frt"
     misc_feature    10362..10861
                     /note="mouse En2 intron"
     primer          complement(10430..10451)
                     /note="LRPCR LAR3_EN2"
     primer          complement(10326..10349)
                     /note="FRT3"
     primer          14527..14545
                     /note="seq primer NF"
     misc_feature    14587..14594
                     /note="RE sites"
     misc_feature    16387..16420
                     /note="loxP"
     misc_feature    complement(14595..14626)
                     /note="Rox"
     misc_feature    complement(16347..16378)
                     /note="Rox"
     misc_feature    14627..14648
                     /note="polylinker"
     CDS             15207..15803
                     /note="puromycin N-acetyl transferase"
     misc_feature    15322..15327
                     /note="Mutated RsrII site"
     intron          10862..11236
                     /note="mouse En2 intron"
     misc_feature    11234..11236
                     /note="Splice Acceptor"
     exon            11237..11290
                     /note="mouse En2 exon"
     primer          11137..11158
                     /note="seq primer FCHK2"
     misc_feature    11291..11413
                     /note="1Dleader"
     misc_feature    11414..11465
                     /note="F2A"
     misc_feature    11561..12277
                     /note="EGFP"
     misc_feature    11471..11560
                     /note="3X NLS"
     misc_feature    14343..14581
                     /note="SV40 polyadenylation site"
     misc_feature    13376..14320
                     /note="ERT2"
     promoter        14659..15170
                     /note="PGK promoter"
     misc_feature    15914..16346
                     /note="PGK pA"
     primer          14401..14424
                     /note="LRPCR RAF5"
     misc_feature    12287..12340
                     /note="T2A"
     misc_feature    12337..12337
                     /note="A to G to kill RsRII site"
     misc_feature    12341..13366
                     /note="Cre"
     misc_feature    11252..11291
                     /note="FCHK lf2a_lacz_gt1"
     misc_feature    11252..11267
                     /note="FCHK QC prefix region"
     misc_feature    11268..11285
                     /note="FCHK QC critical region"
     misc_feature    11286..11291
                     /note="FCHK QC suffix region"
     misc_feature    16443..16463
                     /note="B2 Gateway"
     misc_feature    10250..16493
                     /note="Synthetic Cassette"
     exon            16836..16981
                     /db_xref="ENSEMBL:ENSMUSE00000263002"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000263002"
     misc_feature    16494..17103
                     /note="Critical Region"
     misc_feature    17127..17160
                     /note="Downstream LoxP"
     misc_feature    17104..17183
                     /note="synthetic loxP region"
     exon            18115..19403
                     /db_xref="ENSEMBL:ENSMUSE00000370620"
                     /rank="3"
                     /note="ENSMUSE00000370620"
     misc_feature    17184..21264
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatcctgt cagtatcatt tcctatcctg ggttcaagca ccccaattaa
     4321 ttaaaactct ctgttgtttg gtcagctcgg aggacctggc ttgacctggg gtgagtacag
     4381 tagctctgtc ggtctcccaa gcggttgggt gcccaagctg gagtcctttc tggagtcctt
     4441 tctccgtggc agagcacaga gacacagctg gaaaacaggg acaattagcc agcggctggc
     4501 agctccgggg gtcataaaaa ctcaggaagt ggctaccaca gggctgaggg aatggctgtc
     4561 cctaataaag tgattgtttc aaagcaatgc cagaatgccg gcattcggaa gacacaggct
     4621 gggcctggac ctgggcctca gctccagagc ttaagcctgg gaagggatgt gaagggtgga
     4681 aggagggatt gagtcaggaa ggggagggag atagtcctaa gatcaaaagt ctgcaatgcc
     4741 taccacttaa ccttccacat tctggccacc ccctggggac ttgaacaccc ttcctggtcc
     4801 ttccttctgg aatgaggttt ggttcctcca acctctcacc caccctcagg tttatttctt
     4861 ggaaagtaag atgacaccct tgctgttcct gcaaggtacc tgggagggct ttaaagagga
     4921 gcaacaaggg tccttggctt cttgagggac agctcttgaa gatcaagcca cccccccacc
     4981 cccaccccgg ttttaaccct tcttacaaaa cacagactgt caggaggatg tgcaagtttc
     5041 catagctgta agaaatgcaa gaggtgggaa ttgaggggat aaaggagcct ggacaagtgc
     5101 ccccaagaag gacacacgaa tggacacaca agtgcagggc tccaggcagg agagagcatt
     5161 gtcagcatcc ttgggttgca gacaccatca gctacagcaa agcgcgactg tccagcgagg
     5221 acctgggtag tttggaacgt catgtctgaa gtggcagccc caatctcatc aagctgcctt
     5281 tgcagacaga ccaaagtctc aagagaaaag ggccaaggtt ggctttctcc cacacccaaa
     5341 ggttctcctg tagggttcca acctcagcct tggctcagaa aagtattttc ttccgtagca
     5401 gtaatgaata gcgacaacgg tggattcatt ccgtgggatg aaaagcaaat ttctgcagag
     5461 agagctttca ctttataaag gacagagtgc tgttttaaac taatttaatg ctttaatatt
     5521 tattttatta ttatttatgg gtatggctgt ttttgactgc atgtatgcct ctgtatcact
     5581 cacgtatggt ggctggacac caaaagaggg tgtcatatct acttgggctg gagttctgga
     5641 gttttgagcc caccaaatgg gtactgggaa ttgaacatgg gtcctccgca agagcaggca
     5701 gtgttctggc tgagctatct ctcctcagaa gaaagtgttt taagcatgac ataagacaag
     5761 aataactaga taggtgtcag cacaaatact tcccttgatt aaaacaaaac aaaacaaaca
     5821 aactcttaaa tacactcagg aaaaacacaa gtgtaattgt ggaagtttca aaccgaatac
     5881 gtcgtgtaat tagcattttg cttaagggag agaaatatat caggatccca gccatccact
     5941 aattactgcc ttccatatcg tgggtccagt tgttggcttt gaagagggtt tcgattgttt
     6001 aatgtaatag tttttaaaaa tgaattgctt tccttgaact taaatcttaa ctgtaagaca
     6061 aatcctgggg tggggggcgc aacctggagg tagaatcgtc ttttctcttt tgtagaggcc
     6121 gagtggtttc ctttgacaag gaaataaatg tattgctgag ggttgtccga ccgtccgtcc
     6181 gtcagtctcc tcctggttct cagagcggga aggaatagcc ttcaccgcag ctctacaagc
     6241 cacctacctc ctgggttggt cagaaagctc ttcaaagcaa tgacttccct cccccccccc
     6301 ccctttcttg accaccttct gcctgagaat gtacgaaagg ctgcgcccgc ccgtttccaa
     6361 acccagcttc ccaccaagcc tgcctttctg gacttcccaa ggcgtttgca agtctcttca
     6421 tttcagtctt tgaacctgtg attggaggtt aaagtgcacc caggttggaa ggaggaagct
     6481 cgtagctagc aagaagggcc tgaatattca gctgtgatct ggtcgctgcc ctctcaggag
     6541 cgcccccctc cccaactttt aaaatgcaaa tcgtgtttct gggggtggtg gtggtgggga
     6601 ctgtgtgcgt agcgcgctgc gcctcgacgt ctccagccat tggtgtctgt gtcattacta
     6661 atagagtctt gtaaacactc gttaatcacg taaggccgct ggcctgggac tccgcgagcc
     6721 aacctgcggc gggtcatccc cgcctctaca gcttactggc aaggaggtgg gaggaaagaa
     6781 ggaagagagg ggaggaggca ggacggaggg agggactgcc cgggaggcag aagctctgca
     6841 aggagccgac ggagcaccgt gggctgaggt gcagccagct acctttatct ctagccccct
     6901 gcgcctcgcg cctctggcag ccttcaacgt ttgtccccag acagcatggt gaggtctgct
     6961 ctgggtccct cgccaccatg tacgtgagct accttctgga caaggacgtg agcatgtatc
     7021 ctagctccgt gcgccactcc ggcggcctga acctggctcc gcagaacttt gtcagtcctc
     7081 cgcagtaccc ggactacggt ggttaccacg tggcggccgc ggcggctgct acggcgaact
     7141 tggacagcgc tcagtcccca gggccatcct ggcccaccgc gtacggcgcc cctctccgcg
     7201 aggactggaa tggctacgca cccgggggcg ctgcggcagc caacgcggta gcccacggtc
     7261 tcaatggtgg ctccccggcc gccgctatgg gctacagcag ccccgccgaa taccacgcgc
     7321 accatcaccc gcatcatcac ccgcaccatc cggccgcctc gccgtcctgc gcctccggct
     7381 tgctgcagac gctcaacctc ggccccccgg ggcccgcagc caccgccgcc gccgaacagc
     7441 tgtcccccag cggccagcgg cgaaacctgt gcgagtggat gcggaagccc gcgcagcagt
     7501 ccctaggaag ccaaggtaag cggtgccggg gcgcgcccca ggtcccgcgt gcgttggggc
     7561 tgtcgcgggc tgtctgccag ctcctagcca ggacaggaga aggggcaaag ggggaaatac
     7621 aggtggattc atcccaggag tgtatatttg tgtctcgctc ccccaggttt gtttagggca
     7681 gtaccccatt ggcaacctcg ggacgccttc ggaagctccc ggtagtgtcc taagcactgt
     7741 cgcccgccgc gagctgaatc gtgcccttga gttggctgcc tcccgcattc cttacaccgt
     7801 tcctggccga gagggtcggg ccgcttggat tctctttgcc gtgcctactc aacttagcta
     7861 cctgcttttc ttcttccatc tgaggtaccc ccccgaaagg gccagggagt gtggaggctg
     7921 cgccaggatc agtttggcca ttagggtcta ctgaaggggt cggtgctgct gcgagcacag
     7981 acgccgcggt gcgcccctgg agagaaggcg gagcccccca gaagcctcgg aggaagtggg
     8041 ggaactccag ggtccctgac tcttccctct ctctgccaag caaagtctct catagaaatc
     8101 ctctcttagc atcacagcgg actcggcttc ttaagaaggt ttcttcctga cccttccctt
     8161 ctagaatctc gtgtgtctga gagtgttgtg tgttggattc gggagatttt tcacgaaaga
     8221 cccgagattg ccggtctgtg ggactcacaa gctctgtttg ccctttctga tgtccggatg
     8281 tgtataggag tgggaaggaa gtgagcaaag tgtgacaggg agtggtacta agagtccccc
     8341 cttttttgtt gttccgtgaa gccttctcca gctctttgag cttccgcttc tctggatttg
     8401 ctccttgttg gcacagccag gccacatgcc cgggttatgg ccactttgag gaggtttata
     8461 gccaattaca gccgtataaa tcagagcggc acttgggggc ctgtcgctgg acacttctgc
     8521 gtcactgcgc acaccccgtc aacatcttgg tgggcaatgc tgccggagtg aagtctccag
     8581 attagcctcc agtgggcttt gacattgtca tttccaggag gacttactcc taaaccggtt
     8641 ctccgcataa accctacttt cccaagcctg cctgaaaggt agtgagcgga ggatcgccgg
     8701 taggggtctt agccctccga aagaactttt gcgttcaggg ggtgggtggt ccgagactag
     8761 ggtcctggga gggggaggag aacctcagga gcccaggccg cggggtcagc agatgagagg
     8821 tcgctgtgaa gtcccgcgcc gaggtggcaa gaccgctgtc ggcgccgggc gcccttgtga
     8881 tgttaatgta gggaagctgt ggcccgcagt gccggagccc gactcttctg cagagcgtca
     8941 tctgtcaagg ggcagaggcg tgtacgccgc ccggccggct ttgatgtaca cctttcagcc
     9001 ctgccattgt ctcttttagg gcccggaaag agggtggtga ctttcgcagt caataatgaa
     9061 ttctgacaac tccttctcat ccctcccctc ttccagcccc cctccttggt tcccaggggg
     9121 tgcggggcgc ctgcaagccc ttggggagcg gaccgcaggg ctcacggacc atctttgcgt
     9181 tcctcagcca gcgcgtggtg ctctaagagc agcatccgtt ctagggcgag gaccgctgtg
     9241 ggaaattgtt ctgcgcctcg cttcccaggt ctgatagttt tctttagtac tacaagctct
     9301 ttgctattcg gtctgcacac ttaagatttg gttctgtcgt cttctgtgga gtggggcact
     9361 agcgagtgtg ttacaggctc cagtgagccc gctcccgacc ggttggatcc ctttacttcc
     9421 aaagcaggcg cggggtcggg gccgcagcgc ccattcaaat gcgcctgcgg ggctgtgttt
     9481 atgttaatgc tcctggcggg gaaggggatg aaagctgagg ccgccatttg ctcagtagtg
     9541 gtaattcaaa cagaatgcgg ttgttattaa ggcattgaaa gcgcttttct ttgataagat
     9601 cgccttgtct tgcattgttt gcgactgtgt tcccctttca gcggcggggg gacctctctc
     9661 tgatccctcc tgtatcttcc caggacactt ttgtagtggt ttgcaaaggt ttttacctga
     9721 acaaagtcag cctgcggggc cttaaacacc cctgggcaac tcccacccct tagatctcct
     9781 tgttcaacta ggcactgagc actaaaatct atctgttttg atgtgagacc tctccaactt
     9841 tggacgtgga acagttcctg gccagcattc tgtccattga agcatccccg acttaaccgg
     9901 gttgaatagc cagactcctg cctgatccag cagccccgcg ctatttgtca aggtccttca
     9961 gtaaaacaat caatccaaga tttaatatgt tgactcaatc caatgatcac aggtcacttg
    10021 gtcaggatag ttgcctcggg ggataaatgg gaaattaaaa aagaaagaat gccccaggcc
    10081 aaacctagct gggcaggtga gacgcgttcc aagtagaagg attttcctcc tgacaacatg
    10141 gagatagcac tcagaattcc agggcaggac tttgtgtgtg gtactggtga ctggtgggga
    10201 tctgccagcc catgcatcca tctttccact atattgaaac aaaaggtaaa aggcgcataa
    10261 cgataccacg atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc
    10321 ctattccgaa gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac
    10381 cctccacact tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga
    10441 agaacccgtt gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt
    10501 gggtggcctg cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg
    10561 ccaccaaggt cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga
    10621 agactgaagg ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc
    10681 aaggaaacaa acttggcctc accaggcctc agccttggct cctcctggga actctactgc
    10741 ccttgggatc cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact
    10801 ggctagccta ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt
    10861 ccctaggata gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag
    10921 attttggctg ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc
    10981 ttttagctac atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg
    11041 gtcagtgggc acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt
    11101 atggccttgg gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt
    11161 gggtgcgttg gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct
    11221 cctccatgac aaccaggctc ccaggtcccg aaaaccaaag aagaagaacg cagatcgcag
    11281 atcgcagatc tctagagtca ccgagttgct ttaccggatg aagagggccg aaacatactg
    11341 tccaaggccc ttgctggcaa tccacccaac tgaagccaga cacaaacaga aaattgtggc
    11401 accggtgaaa cagactttga attttgacct tctcaagttg gcgggagacg tcgagtccaa
    11461 ccccggaccg gtgcatgtgg accccaagaa aaagcggaag gtggacccta aaaagaaacg
    11521 caaagtggat cctaagaaaa aaagaaaggt gcacgtgtcc atggtgtcca agggcgagga
    11581 actgttcacc ggcgtggtgc ccatcctggt ggaactggat ggcgacgtga acggccacaa
    11641 gttcagcgtg tccggcgagg gcgaaggcga cgccacatac ggaaagctga ccctgaagtt
    11701 catctgcacc accggcaagc tgcccgtgcc ttggcctacc ctcgtgacca cactgaccta
    11761 cggcgtgcag tgcttcagca gataccccga ccatatgaag cagcacgact tcttcaagag
    11821 cgccatgccc gagggctacg tgcaggaaag aaccatcttc tttaaggacg acggcaacta
    11881 caagaccagg gccgaagtga agttcgaggg cgacaccctc gtgaacagaa tcgagctgaa
    11941 gggcatcgac ttcaaagagg acggcaacat cctgggccac aagctggagt acaactacaa
    12001 cagccacaac gtgtacatca tggccgacaa gcagaaaaac ggcatcaaag tgaacttcaa
    12061 gatccggcac aacatcgagg acggctccgt gcagctggcc gaccactacc agcagaacac
    12121 ccctatcggc gacggccctg tgctgctgcc tgacaaccac tacctgagca cccagagcgc
    12181 cctgagcaag gaccccaacg agaagaggga ccacatggtg ctgctggaat tcgtgaccgc
    12241 cgctggcatc accctgggca tggacgagct gtacaagcct gcaggcgagg gcagaggaag
    12301 tcttctaaca tgcggtgacg tggaggagaa tcccgggccg tccaatttac tgaccgtaca
    12361 ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat
    12421 ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt
    12481 ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc
    12541 tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat
    12601 ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tctgggctgc cacgaccaag
    12661 tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg
    12721 tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact
    12781 catggaaaat agcgaccgct gccaggatat aaggaatctg gcatttctgg ggattgctta
    12841 taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac
    12901 tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg
    12961 tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc
    13021 tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc
    13081 cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac
    13141 tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg
    13201 acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga
    13261 gatcatgcaa gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct
    13321 ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgatctcg agccatctgc
    13381 tggagacatg agagctgcca acctttggcc aagcccgctc atgatcaaac gctctaagaa
    13441 gaacagcctg gccttgtccc tgacggccga ccagatggtc agtgccttgt tggatgctga
    13501 gccccccata ctctattccg agtatgatcc taccagaccc ttcagtgaag cttcgatgat
    13561 gggcttactg accaacctgg cagacaggga gctggttcac atgatcaact gggcgaagag
    13621 ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc caccttctag aatgtgcctg
    13681 gctagagatc ctgatgattg gtctcgtctg gcgctccatg gagcacccag tgaagctact
    13741 gtttgctcct aacttgctct tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga
    13801 gatcttcgac atgctgctgg ctacatcatc tcggttccgc atgatgaatc tgcagggaga
    13861 ggagtttgtg tgcctcaaat ctattatttt gcttaattct ggagtgtaca catttctgtc
    13921 cagcaccctg aagtctctgg aagagaagga ccatatccac cgagtcctgg acaagatcac
    13981 agacactttg atccacctga tggccaaggc aggcctgacc ctgcagcagc agcaccagcg
    14041 gctggcccag ctcctcctca tcctctccca catcaggcac atgagtaaca aaggcatgga
    14101 gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc tatgacctgc tgctggaggc
    14161 ggcggacgcc caccgcctac atgcgcccac tagccgtgga ggggcatccg tggaggagac
    14221 ggaccaaagc cacttggcca ctgcgggctc tacttcatcg cattccttgc aaaagtatta
    14281 catcacgggg gaggcagagg gtttccctgc cacagcttga tgaagatctg agctcttaat
    14341 taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc
    14401 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    14461 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    14521 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    14581 ggatccgttt aaactaactt taaataattg gcattattta aagttacata tggcggccgc
    14641 cctaaggctc gacctcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg
    14701 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
    14761 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
    14821 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
    14881 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
    14941 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
    15001 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
    15061 ggggcggggc gggcgcccga aggtcttccg gaggcccggc attctgcacg cttcaaaagc
    15121 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatccatc
    15181 tagatctcga gcagctgaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
    15241 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
    15301 ccacgcgcca caccgtcgat ccagatcgcc acatcgagcg ggtcaccgag ctgcaagaac
    15361 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
    15421 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
    15481 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
    15541 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
    15601 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
    15661 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
    15721 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
    15781 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
    15841 ggagcgcacg accccatgca tcgatgatat cagatccccg ggatgcagaa attgatgatc
    15901 tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa
    15961 gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg
    16021 gggtgggatt agataaatgc ntgctcttta ctgaaggctc tttactattg ctttatgata
    16081 atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat
    16141 tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg
    16201 attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa
    16261 gaacgagatc agcagcctct gttccacata cacttcattc tcagtattgt tttgccaagt
    16321 tctaattcca tcagaagctg gtcgagtaac tttaaataat tggcattatt taaagttagg
    16381 cgcgccataa cttcgtatag catacattat acgaagttat gtcgagatat ttaaatatta
    16441 gacccagctt tcttgtacaa agtggttgat atctctatag tcgcagtagg cggtggcctg
    16501 ctgtttcatt tctaacaagt ctgaaaatgt ggatattgat attcctgggg actctggtca
    16561 ctttcccacc gccctttttt ctgatcctaa atatactata tttgatttat ggaaaggagg
    16621 ggtgctgtgt gagaggtgtt cctttcaggt tagttgtgcc tccaggctta cacccccctt
    16681 ccccaggatc ctgtgagtgg atattgtcaa cctgggtagt agacaccccc ctccatcagt
    16741 ggatggagag aaatgactcc tgggttaggg aggtttgtca ttaccaatga ctgatggatt
    16801 gtagttctta gccaaaactt ctccttcctc cacagtgaaa accaggacaa aagacaaata
    16861 ccgggtggtg tacacagacc atcagcggct ggagctggag aaggagtttc actttagtcg
    16921 atacatcacc atcaggagga aaagtgagct ggctgccaca cttgggctct ccgagaggca
    16981 ggtgggaatc ggctttcctg ggtctggtta ttatggcctg gggcaatctt aatgggtagc
    17041 attagaaagt ttggtcgagg gagctgttgc cttttgagaa tccttgagtg tccagttgtt
    17101 tttgagatgg cgcaacgcaa ttaatgataa cttcgtatag catacattat acgaagttat
    17161 ggtctgagct cgccatcagt tcaaaaagcc aagtcgaggc tacagggtgg aactgcactt
    17221 ggacagagaa agagcgattg gaaccatggt tctcaacctg tgggtcgcga cccctcagct
    17281 tgccactcaa acacgctgtg ctttataata gcagcaaaat tatagttatg aaatagcaat
    17341 taaaataatt tgtgattggg ggtgtcacca caacatgaag aactgtccta aaaagaaaaa
    17401 caaaggtcca gtctacgcat acaaactaaa gtctgctcat acaaattaaa gaggcagggg
    17461 ctggagaggt gatgcttaag aacacacact cttgcagagg atcggagttc tgtttccagc
    17521 atccacgtca ggtggctcac agtgtcacag tgtcggctaa ctctgattgc aggggacacc
    17581 cagtgcctct ggcttctgtg gatatatatg aatgtataca gacatacaca tatcaaagat
    17641 ttaaaaatcc ttgaactttt tttttcatta acacaaaaga ggccctactt gcacaccaac
    17701 actttctgta gtctagggaa aagggaagga acaaacaaga gaaattcata gccaggaagt
    17761 tttggacaaa gtttcattgt ctgtgccaag gtgaagaaca tcgcagtacc aatgagctgt
    17821 gggagcccaa gggtgaccca cgaggcacac tgagctggcc aaccatttgt gtagggaata
    17881 gctttcgtca tggttccgtt ccctggttct gaggttctgt tgctagtaaa gggttttttg
    17941 tttgtttgtt ttttgttttt tgaaagcaac ttggggaggg ggcaggtaag taaagaggac
    18001 gaggctggcc tggctttggg aacatttccc aaactcagtg aaagaaggac gctcccgggg
    18061 tggcgccatt tctctgttct tatcttcttc taccttctat tttgtcatct ctaggttaaa
    18121 atttggtttc agaaccgcag agccaaggag aggaaaatca agaagaagca gcagcagcaa
    18181 cagcagcagc agcaacaaca gcctccacag ccgccgccac aaccttccca gcctcagccg
    18241 ggtgccctgc ggagcgtgcc cgagcccttg agtcctgtga cctccttgca aggctcagtg
    18301 cctggttctg tccctggggt tctggggcca gctggagggg ttttaaactc cactgtcacc
    18361 cagtgacccc tcccgtggtc tgaagcggcg gcggcacagc aatcccaggc tgagccatga
    18421 ggagtatgga cgctgcgaga atcctcagaa gagattcctc tcctcctacc cacgaacagc
    18481 atctactgat ggagattgag gacagaagat gagtggaatt atggacctca ggggaagaca
    18541 tggtttagat tttttttttc tttttaactt ttcccattcc gactcttcct gccagcaacg
    18601 acaaacgaag tgattcctgg ggcttcttcg ttcatgctct ttgccaggac tgactaccga
    18661 catgaagcta tcagcctctt ttgccccagc tctttgcctc tctgtatttc tgtgtggagc
    18721 tgaggagaga gtgagactgg atggggtggg ggtagcaata cttgagccaa ggtggctgtt
    18781 tcctgctgac tgctttctga gaaccagctg gccgtcctgc ctccgggcca gggactattc
    18841 aaactacagg agccagaggc agctaagata gctggactga ccgaagtctg cagaacctcc
    18901 cccaccaggt ggtctgggct ttcttctcca caaatcagga aggggtggtg ggttcagggg
    18961 ctgcggtgag agggggttgg ttagccaacg ccaggcccct gcgacaaggg cttgtttaga
    19021 aagcctgtca ccagagctgc tgtaggcgga atgtatgtct gtgttgtaaa tgccagagcc
    19081 aacctggact tcctgtccct tccctcgtct ttggctgaag aagaccggaa ttgtttgctg
    19141 ctgttcgagt cactgatctg tgtaacgagc caaacaagcc ttttaaaaag ccttcttgat
    19201 ccatgggtag agaagttgta tggtgaaggg aagtcgggag ggggggaagg ggatccgaac
    19261 acagttgact tttattttgt aaaaagacaa agataaacga actttaacag atttcagatc
    19321 ctggatttta tcttctttgg attttgactg aaatttttat tattattttt ccttaggaaa
    19381 taaaacatct tctctggttg caagctagac gtgtaggggt ggaggtgatg gggtagagaa
    19441 ggttgtctgg gcagcccatt tttttaccca ggagctgaag gagcaagtcc tgagccttcc
    19501 cccacacccc atgcctccgt ccagcaatgg gctgtggaat gtgtgctttt cctgggggtg
    19561 cctttgggca aaaagctcgt cacaacggct agctgcttcc agctgctgca gaatcattcg
    19621 tgggggtggg gtgagagggc tttagctccc ttctcaatgc aaacagaagg caacttcctc
    19681 cattagcgac ccccaaccat cccagccacc gcgcgctcag gtatgcccct tagccaatca
    19741 aattgagcgc agcattgact actatttttt ttttcctgtt ctttcccgac gacgctttta
    19801 tgacagatag tcctctgaat tcctaggtat ttccatgggc cccctctgcc tacactcctt
    19861 tctaactacc cggaataaca ttatcttccc actcccacct ccaccaaaca caagaattca
    19921 agatcagaat aagatccaag aggtggctac ttatctgcct gatctgctgc ctctcctgag
    19981 tgtctgggtc ctctggctag gccagaaagg aaaactttac cacttccact taggactaag
    20041 ccgtgcccta aggtactgga ttcacatgga gggaggaggc aggcaggcag ggcagggagt
    20101 ccaccacccc accacccggc agcacctccc cagtttctcc actctaacag gtggcgaatt
    20161 tgctgttaga ttgggaaagg tgtcttctgt aggaaaggaa gggtgttggt cactcagggg
    20221 ggaagctgcg tgggtgtctg cggggcgggg gctccccagg ttcagggcca ggtgatcctc
    20281 gttgccccca ggtgcttccc gccccagcta ggatcgcagc cctgcgcggt ggccgcgccc
    20341 acacgtcagc tcctcgccgg cggcgcggtt gctatgcgtt gccgtgaaac gcctgtcaat
    20401 aaactctgtt tggacagtgg agcaagagca caagggttgt ttgcttagtg gttagaggtt
    20461 caaaagtcgg cattgtcccg ctgcacgcga ttacaagtcc ccccacccac ccttcccggg
    20521 gctctctggc gcagagatga tggggggggg gcgctctgaa acgcaaccaa gctcgctgta
    20581 gcagaccctc cccctctccc gctttctgta gcctgagcct cccaggccaa gggagcagga
    20641 aaaaaaggcc cttccgggcc agtgacgctg tagagagctt cagacccaga gacactgtgt
    20701 catcggtaaa gccagctgga ggaaacgtgg gatgtggcgc gaagtggcag accagcctca
    20761 gtttagagag acttgtacct ggagcttgag gctgttttag agcgaataat ggagaggctc
    20821 tataatgctc cagctccggg cccttgagac atcagctctg ggttcaggcc gctaggaggc
    20881 gcgtgtgctc tgggaagagc tggggattgg tctgtgctct gcgctctttg cttggagaca
    20941 cctaaaaggg ctccagatcc cgcccaacta gaaaatttca gccaacgcgt ctcctcacct
    21001 cagctgctgt cacaccaagg cacccgacat ctcccaggat agttcttggc ttgtagaaaa
    21061 cgagttaggg ttgaaggtgt agggatggga cataacgcac taacaggctt ggcatgtgag
    21121 aaggttcagt ccccagagct cacagtccaa acgcccaagt tcagactgcg ggagattctt
    21181 ggatctgttg actttaagaa aacaaacctg gaataaagat tcctcaccat tctaaaagag
    21241 cccctttgta ccgtttctct cttt
//