LOCUS       KO-first_condition_ready_25876_MGI:1336882        20125 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Vamp8 targeting project(s): 25876
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_gt0
COMMENT     design_id: 43248
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     exon            6967..7196
                     /db_xref="ENSEMBL:ENSMUSE00000698105"
                     /rank="1"
                     /note="ENSMUSE00000698105"
     misc_feature    4112..8830
                     /note="5 arm"
     misc_feature    8858..8882
                     /note="B1 Gateway"
     misc_feature    8895..8942
                     /note="Frt"
     intron          8943..9817
                     /note="mouse En2 intron"
     misc_feature    9815..9817
                     /note="Splice Acceptor"
     exon            9818..9857
                     /note="mouse En2 exon"
     misc_feature    9852..9859
                     /note="Frame 0 insert"
     misc_feature    9860..9913
                     /note="'self-cleaving' 2A peptide"
     gene            9914..12991
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    12992..13045
                     /note="'self-cleaving' 2A peptide"
     gene            13046..13837
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    14036..14278
                     /note="SV40 polyadenylation site"
     misc_feature    14295..14342
                     /note="Frt"
     misc_feature    14349..14382
                     /note="loxP"
     misc_feature    9832..9933
                     /note="FCHK std_gt0"
     misc_feature    9832..9849
                     /note="FCHK QC prefix region"
     misc_feature    9850..9915
                     /note="FCHK QC critical region"
     misc_feature    9916..9933
                     /note="FCHK QC suffix region"
     misc_feature    14398..14418
                     /note="B2 Gateway"
     misc_feature    8831..14448
                     /note="Synthetic Cassette"
     exon            14847..15005
                     /db_xref="ENSEMBL:ENSMUSE00000388617"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000388617"
     misc_feature    14449..15208
                     /note="Critical Region"
     misc_feature    15232..15265
                     /note="Downstream LoxP"
     misc_feature    15209..15288
                     /note="synthetic loxP region"
     exon            17640..18070
                     /db_xref="ENSEMBL:ENSMUSE00000804254"
                     /rank="3"
                     /note="ENSMUSE00000804254"
     misc_feature    15289..20125
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg acccagcact ggaatccaaa gacaaaagag
     4141 taaagttgtt attagttgtg tagtttacat tttctagcag agaggctgga ggtgcagtgt
     4201 gaatgtggct cgggaggtcg agggctcctc tggcactgca gggagcactg gactctcttt
     4261 ccagctagga aagaaaatgt ggctgactgg ctgattgatt aactgactga ctgattgatt
     4321 gatgaatgat tgactggaga cacagtctgg ctttgtagcc caaggtagcc ttgaatgtgt
     4381 gattgccccg gctcagattc ctgagttctg gagcagcact gcacaaggct gtaaaacgtt
     4441 catgacattt atgaaagtag cgttcttctt ttcgaactaa gttgctgtaa tctgttgtgc
     4501 attttccagc ctccgttctc actagcagcg ctgggagagc tcactggccg tatggagtca
     4561 gcgttgtact gggcagcaaa agaacagagc aagcatttgg gggtgggagg tcttgggaca
     4621 gaacccaggg ctttggcgat tctaggcaaa ctctgttgct actgagagag ccttcgacac
     4681 tgagacaggg tcttgagacg tagtcctgcc tgtcctgtat ctcattttgt agactagctg
     4741 gcctcccaga gatccccctg cctctgcctc ccaagatgtt aaaggcattc gtcaccacac
     4801 tggcccaggc aggttctgtc ttataaacct ccttgtgatt taagatgaat cttttaaaat
     4861 atagagtttt tttttttttt ttatggtttt ttgtttctgt tttttgtttt tttcgagaca
     4921 gggtttctct gtgtagccct ggctgtcctg gaactcactt tgtagaccag gctggcctcg
     4981 aactcagaaa tccgcctgcc tccgcctccc aagtgctggg tttaaaggcg tgcaccacca
     5041 ctgcccagct actgttacct tttaactatg tatgtctgtg tttgtgtgta tgaatagggt
     5101 ctgtggggcc agaagagggc gtcagatccc tggagctaga gttacaagta atgagttgcc
     5161 tgacccaggt gctgggaatc aaatttgggt tctctggaag aacaatacat gcttttaacc
     5221 aaagggccat ctctccagga cccagttctt tctttgtgac cactgagccc ttgatcagca
     5281 ggatggggac attctcaagg cctcttgacc ttttgcctaa gatcaagtgt agtacagaca
     5341 ttctctgttg tgatctcatt cctctttatt ctagattttt cttttgcttt ataggaaagg
     5401 aaaggagttg tgccgcttta aggtgttact aggggctaga gagagagctc tacagctaag
     5461 agcacgtgct actcttgcat agaagggtcc agctcccagc acccacatca ggtgtctcac
     5521 aactgtctgc aactcaccag agaacctaac ccctctgccc tcaggcacac acacacacac
     5581 acacacacac agacacacac acagacacac acacacacac ttaaagataa taagataaaa
     5641 acttttagcc gggagtggtg atgcacacct ttaatcccag cacttgggag gcagaggcaa
     5701 gtggatttct gagttcgagg ccagcctggt ctacaaagtg agttccagga cagccaggga
     5761 tatacagaga aaccctgtct cggaaaaaaa aaacaaacaa ataaacaaaa aaaaaaaaaa
     5821 cccaaaaaaa aaacccaaaa aaaccaacaa caacaaaaaa aatttttaaa gattttttta
     5881 aagtgttact agttgcttta atttgcttct gtaaaactaa gcgtgcagtg tttgtagata
     5941 tagctcctcc atggctgcct gagcaggcac gagggaaaac acataagtgt cccaaaatga
     6001 ttccgggatt ctaaatttat tatagccaac aaaatgaaac aaaacaaaac ccctcttatt
     6061 tgttggagga gctaagattt cctccttgac ggtgttattt actctggggt gggaaagaat
     6121 gtctgttctc tttctgccct gttattgttc tgtcttgaca aactccatcc ggagaagagc
     6181 agattttctc tggcggagag ccacatgtgg tggaaaaacc ctttactctt aggcccccat
     6241 cctaatctgt ctgacaaccc tctgacaact gaccagggtc accaaccccc acggtgacct
     6301 ttggaaggaa gtgtaggact gctcaactcc agaaggaaag gaggggctgg tgagtgagcc
     6361 atttccccga ctgtcctgtg tcctgtgaca aggataaggt gccctgcccc tgctgtttgc
     6421 actcctccca ctgcctgagg tcagccttag cacccgaaga acattagagg gggacaaaga
     6481 tcccaccccc agggccaact tggaattttc ctggagattt taaagacttt aattatgtgt
     6541 atgtgtgtgt atgtatgcac acgtatgttc acatacccac agaggccaga agagggcatc
     6601 tggaccgccc ctgccccccc cccccccccg agatgcagat acaggtgtct gtgagcagcc
     6661 aggggtgggt gtagggacct gaacctgggt tactgcaaaa gcagcgggtg atcccaactg
     6721 ctgacccatc tccctagccc ccgttttctg ggggatctta gagctctctt ctcatacagg
     6781 atggcccttg gggacaaagg ttgcccaaca aggtatcttc gcctgtacat tcgtttctat
     6841 ctaaataatg ctaatgtaag tccctgaaaa actagaatta cttctttgaa gcataaaaga
     6901 agtttctgaa aacttaggag gactgttgat ccttcttttc tccttccccc ctcccccaac
     6961 cctgttgctt aagcttttgt ttgggaggag actaggagga cggcagcaga gggcagccgc
     7021 ctacctggag aaacctgcag ttacgtgtgt ggcaggagcc ggagtggctg gagtcgcggg
     7081 atggtcagga ggcgggctga aagttggagt aagcaggaag tgaagctgtg gctgccttgg
     7141 gtggaaacag actaggcgaa gttctgcttt gagaggccca agtgctccga gacatggtga
     7201 gtgacgtggg gaccaagaaa agagcgagtt agagcagctg ccagaggggg atcgggcctg
     7261 ggggttgcct gtgtcgagaa aggtaagttg gcggcagtca gagggctggc ggggttgggc
     7321 cagcgctgcc tctgtctcct tacttttgac aactcctcca caaatagctc cgcactccgg
     7381 gccaggggtt cctgacactt ctccacctcc ttcctcccgt gacctcggtg ctggatccga
     7441 tcaggatttc ggtgggggtt gggggaccca aattccaagc cgagctgctg cgccgcgccc
     7501 tccccctcct ccgcctcctc cagctgggcc tgagcccggt gttgtgacat taccgggcca
     7561 caagcgttcc tgctctttgg ggctcagatt ccagccccgc ggactggagt tctccccctt
     7621 accctcgcac tgtatctatg cagacaccca gaggatctat ctgcctggcc accaaaagca
     7681 ccaccctttt ctagaaacat tagcaacagt gccaagggtt tgtaaagcag ccagatggcg
     7741 gttcttcccg ataatctagc ttcagcccac acccgtggcc ggtacctgag gccccctggg
     7801 tccctaaggg aggagtcagg cctcttccct ccttgttcaa aagccttatc tagaggcctc
     7861 ctggatagag cgaccgcagc aggaaggact tttattaata tctgcataag caggcggtgg
     7921 tgtgcggcct accaggcgtt aaattgtggg gaaagcagaa gtatatggtc caacggcaca
     7981 tgatgccttc tgggggccac atccactgaa ccggggctac actgcccagt aggctggaac
     8041 cagaactcac caacagctgt ctggagaagg gcatttattt gggttgggcc gggcaagagt
     8101 gtgctgctgg tcaactctca cagttttaga gagaacccac taggattgag ccgagggagg
     8161 ctacaggaac cagttaatag ttcttggctg tgctctgctg aggaaacgtc agctgggttt
     8221 tctccttgac cctctttgtg gagtcaagac aggggcagtc gtgtgctgtt tttttgtttg
     8281 tttgtttttt gttttttgtt tttttttgtt tttccttgtc acgctgaaaa tcctccaaag
     8341 aaggtggatg agaatccagg gtcaaacctc atcttctggg acttaaaaaa tgcacttaca
     8401 tggctggcag ccgagatggc tgggcaggta caagtgtttg cagagcaagc ctgcctgcct
     8461 gaattcagta tcctgctctc tcctgcccat ccaactggag gagacaatcc actcatgaaa
     8521 gttgtcctct cacctccaca tgcgtgccgt ggcatgtgca tacacgtata ttccccagca
     8581 cacacacaca cacacaatgc acttatgaca gactaaggat gtggttcagc tgatagagtg
     8641 tttgcctggc aagcgtcaag ctctgggttt catccccagc acctcataaa acaaggataa
     8701 tggtacacac ctggaatccc agcccagggt aagtagagac agggggtcag atgtttaagg
     8761 ccatcctact taggaagcct atggcctgcc taggttatat gagaacctgc ctcagataaa
     8821 taaatctggg aaggcgcata acgataccac gatatcaaca agtttgtaca aaaaagcagg
     8881 ctggcgccgg aaccgaagtt cctattccga agttcctatt ctctagaaag tataggaact
     8941 tcgaaccctt tcccacacca ccctccacac ttgccccaaa cactgccaac tatgtaggag
     9001 gaaggggttg ggactaacag aagaacccgt tgtggggaag ctgttgggag ggtcacttta
     9061 tgttcttgcc caaggtcagt tgggtggcct gcttctgatg aggtggtccc aaggtctggg
     9121 gtagaaggtg agagggacag gccaccaagg tcagcccccc ccccctatcc cataggagcc
     9181 aggtccctct cctggacagg aagactgaag gggagatgcc agagactcag tgaagcctgg
     9241 ggtaccctat tggagtcctt caaggaaaca aacttggcct caccaggcct cagccttggc
     9301 tcctcctggg aactctactg cccttgggat ccccttgtag ttgtgggtta cataggaagg
     9361 gggacgggat tccccttgac tggctagcct actcttttct tcagtcttct ccatctcctc
     9421 tcacctgtct ctcgaccctt tccctaggat agacttggaa aaagataagg ggagaaaaca
     9481 aatgcaaacg aggccagaaa gattttggct gggcattcct tccgctagct tttattggga
     9541 tcccctagtt tgtgataggc cttttagcta catctgccaa tccatctcat tttcacacac
     9601 acacacacca ctttccttct ggtcagtggg cacatgtcca gcctcaagtt tatatcacca
     9661 cccccaatgc ccaacacttg tatggccttg ggcgggtcat cccccccccc acccccagta
     9721 tctgcaacct caagctagct tgggtgcgtt ggttgtggat aagtagctag actccagcaa
     9781 ccagtaacct ctgccctttc tcctccatga caaccaggtc ccaggtcccg aaaaccaaag
     9841 aagaagaacg cagatctccg agggcagagg aagtcttcta acatgcggtg acgtggagga
     9901 gaatcccggc cctgggatct ggactctaga ggatcccgtc gttttacaac gtcgtgactg
     9961 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    10021 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    10081 cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg
    10141 cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga
    10201 tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac
    10261 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga
    10321 aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg
    10381 gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt
    10441 tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta
    10501 tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca
    10561 taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag
    10621 ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt
    10681 aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg
    10741 tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga
    10801 aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca
    10861 caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg
    10921 gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg
    10981 tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat
    11041 cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc
    11101 gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga
    11161 aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat
    11221 gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg
    11281 gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa
    11341 atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac
    11401 cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc
    11461 gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg
    11521 cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt
    11581 tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat
    11641 taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc
    11701 gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc
    11761 gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat
    11821 cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt
    11881 ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg
    11941 taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct
    12001 cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc
    12061 ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc
    12121 catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg
    12181 gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact
    12241 gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag
    12301 tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta
    12361 ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat
    12421 tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta
    12481 ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac
    12541 accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa
    12601 ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga
    12661 ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg
    12721 tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca
    12781 gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct
    12841 gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga
    12901 cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta
    12961 ccagttggtc tggtgtcagg ggatcccccg ggagggcaga ggaagtcttc taacatgcgg
    13021 tgacgtggag gagaatcccg gccctattga acaagatgga ttgcacgcag gttctccggc
    13081 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
    13141 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
    13201 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
    13261 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
    13321 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
    13381 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
    13441 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
    13501 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
    13561 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
    13621 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
    13681 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
    13741 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
    13801 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
    13861 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
    13921 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    13981 gatctcatgc tggagttctt cgcccacccc ccggatctaa gctctagata agtaatgatc
    14041 ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    14101 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    14161 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    14221 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgg
    14281 gggtaccgcg tcgagaagtt cctattccga agttcctatt ctctagaaag tataggaact
    14341 tcgtcgagat aacttcgtat agcatacatt atacgaagtt atgtcgagat atctagaccc
    14401 agctttcttg tacaaagtgg ttgatatctc tatagtcgca gtaggcggta cgcaagcatg
    14461 aagatttgca ttggatccct agacccattt agaaggaaaa aaattccagg tgtggtggtg
    14521 caagcaagca cttaggtggc tcctagtgct agggtggtgg agaaggctga gccctggggc
    14581 tttttgtcca ctctaatctc agagcctcag gccaacagga gacccagcct gtgggggaaa
    14641 aaacaaggga ggagtgacac ctgaccaccc gaggctgatc tgggatataa acgtgcttgc
    14701 atcgaagtga acatttacgt agaacattct cctggtaaac agttgaagca tcagaaacgg
    14761 ctaaaatcta ctttagttct tccaacctcc tctggcctct tggatcctct gctgcccggc
    14821 ctaattaacc ccttattgac atccaggagg aggccagtgg gagtgccgga aatgaccgag
    14881 ttaggaacct gcagagtgag gtggagggag tcaagaatat tatgacccag aatgtggagc
    14941 ggatcttggc cagaggggag aacctggacc acctccgaaa caagacagag gacttggaag
    15001 ccacggtgag attgggagaa aactgggagt tggtgggggc agatctcggg gagggggaga
    15061 agtttgggtg gggaggcaaa gtgaacagaa gggactgcag actgtcatgt ctactgccct
    15121 tttgcctatg caggagatgg ctgaggttcc acctctgctt ccttgctact ctgctggctg
    15181 gtagagagcc cagtgagtgg ttggatttga gatggcgcaa cgcaattaat gataacttcg
    15241 tatagcatac attatacgaa gttatggtct gagctcgcca tcagttcatg ctccattcct
    15301 aagcttcatt ggggtatgaa actgattttt gtctcaaagc ttgcccttgg caaccatttg
    15361 gagttgatgt cccaaaggca aagttatgag ttccgggcaa cccttggcct ttcagcattc
    15421 tccctccagg tgtaattagg ctggaagttg gtggggggcg tcttgctgag tccgctttaa
    15481 agcacagctg ggaagaccaa tgaaatcctt taaccccagg atccctgtgt gcaaaggcca
    15541 aaataatatc tcccacacat cttgcttaga cctcctcttt gctgccatgg actgaggcca
    15601 agctgctcag ggcagaagaa agtctaacac aaaccaaggc tggggcctca gttttcactt
    15661 gctgtggagt acactggctt cctgggtcca ttgggttatc ttggaatcta gtgcctcctg
    15721 cagcataagc tgggcacatg gagacttagt gtctgtatta acttggagaa gcaggaggct
    15781 tttttctttc ttcctttctt cctttcttcc tttcttcctt tcttcctttc ttcctttcct
    15841 ttcctttctt cctttcttcc tttcttcctt tcttcctttc ttcctttctt cctttcttcc
    15901 tttcttcctt tcttcctttc ttcctttctt cctttcttcc tttcttcctt tcttcctttc
    15961 ttcctttctt cctttcttcc tttcttcctt tcttcctttc ttttctttct tttctttctt
    16021 tctttctttc tttctttctt tctttctttc tttctttctt tctttctcaa gacagggttt
    16081 ctctgtgtag ccctggctgt cctggaactc actttgtaga ccaggctggc ctcgaactca
    16141 gaaatccacc tgcctctgcc tcccaaatgc tgggattaaa ggcgtgcgca accacgccca
    16201 gctctttctt ttaagtttct gcagtgcttt ggttcttcct tcagcttcta cagaactcag
    16261 aagacaaagc aaagtatcat tgagccagga catgtgccca tctggagcac attcctgtac
    16321 cccttagctt gtgggtctgt gctccaaagg ccagatttct ctaccagtca aacaaactcc
    16381 aattgtgagc atatatctgt aaaagacccc ttagtatgca ggttagtgca ggggcaagaa
    16441 aaagaaggga gactggacat aagactcagg tggtagagta cttcactaga cttcacgaag
    16501 ccctgggttc agtccctggc actatataaa tatggtattg tggcacacgc ctgtaatctg
    16561 aactctttga aggtagaggc aagtggatca gaagttcaaa gccaggccat tgagatggct
    16621 caaaaggtaa agacacttgg taccaagcct aaggatttga gttccatttg tgggattcat
    16681 atggttggta gtcttgattc ctataaaatg acctctgacc tccactgtgg cttgtgcaca
    16741 ccacacacat aaacaaacaa acaaataaat attgttttta aaagttcaag gtcgccgggc
    16801 atggtggatt aatcccagca ctcgggaggc agaggcaggt ggatttctga gttcgaggcc
    16861 agcctggtct acagagtgag ttccaggaca gccaggacta cacagagaaa ccctgtctcg
    16921 aaaaaccaaa caacaaacaa acaaacaaac aaacaaaagt tcaaggtcat cctttgctac
    16981 aaggagtgga ggccaggctt tgctgtatga gatcccgact agaaagatag aaacagaaaa
    17041 ggaagggacc ctgctatgct cagagggaag ttttatgagt tcaagaattc taaactagaa
    17101 cttggcttac agattgttgt agaaagacat ttgtgaagca gccagagata gacagatctc
    17161 tgaagctcct ggccatccag gcatacttag tgaggccctg tctcaaaagt aaaacaaaac
    17221 aaaacaaaac aaacaaacaa aaaaaaaaaa aaccagagag agatcagtgg gtaaagactt
    17281 ttgtcagcga gcctggctgc gtgggttcaa tccccaggac atacagagga gaagagagaa
    17341 ccatttcctg caagtcgacc cctctccacc acgtgggcag tgtggcacat aaggcagagg
    17401 cacaaataca tgttataaag tatttttaag aacctttcca ttcctattgt aagaggaaag
    17461 aattgttcac ttatttgtgt ttttatttcg aagtggctat cctccagctc cagcgtcttt
    17521 ttttccgttt cctacttcga tttataatgt aatgtaaaca tctatactat ggcatgtggt
    17581 gtccgtctgt actctcagtc tgagggcagg agctcacgac tttatcttat ccccaacagt
    17641 ctgaacactt caagacaacg tcccagaagg tggcccggaa gttctggtgg aagaatgtga
    17701 agatgattgt catcatctgt gtgattgtcc ttatcatcgt catcctcatt atactttttg
    17761 ccactggtac catccccact taagagtccc ttccatccca ccctactcct caggggtgac
    17821 ctccctaata tgtgccaaga ggagccctct cctgtcctcc tccacccaag tgctgctcag
    17881 tccctgtgag ggccttctgc tgcatcagat gccccagacg caccttgatc ccctgggagg
    17941 agacagctgg actgtagctg catctcatgg tcctgagagt gggttacaga gacccagagg
    18001 accctgtctg tggctgggaa actgttggtg gctggtgggc aataaagacc tcttggtatc
    18061 cctaagtgta cgaggcgctt tactcctgtg tctttcaagt gtctgtttct ttctttctga
    18121 ggaacccctc caggctggtt tctcccaatc tagaaccaag atagtgcaag gattgagcac
    18181 aggtttagtg aagaaagatg gcagctcaga ggctttgcat acctgaatta gatatgccaa
    18241 tcactagacc cagctagaat caataagtca gtcagtctgt ctgtctgtct ttaaaaggac
    18301 ttttattttt taagaagaat gagtatgggt ttagagagat ggctcagagg ttgagagcac
    18361 tgactgctct tccagaagtc ctgagttcaa ttcccagcaa ccacatggtg gctcacaacc
    18421 attctgtaat gggatctgat accctcttgt ggtgtgtctg aagacagcta cagtgtactc
    18481 atagacacta aaaaaaataa agaaaattat ttaaaaaaat aagtatgagt atgtgaatga
    18541 agagcctgca gaggccagaa gaggatgttg gttcccctgg agttacaagc ccaacatggg
    18601 cgcttggaac caaactggag tcctctgcaa gaacagtttg cactcttccc cccttcccct
    18661 taggtaaaag tttatgtttg attattaatt aaaatataat tatattgtgt tgccccttcc
    18721 ctttcctctc ttaacacccc tcaactcatc atcctccctt cacttcctct caaattcacg
    18781 gcctctttct ttcattgttt tacacacaca cacgcataca cagtgtgcag atttgtgttt
    18841 ggttttgctc gcatgtaaat aattacaggg ctgagtactt ggtgttgata accaattaag
    18901 aatctcaact ctggagaatt gttgttcctc ccccctctgt gctagcatgc ctgttgatgt
    18961 ccttgctcag atcttgttta ggcagccata ttgttgaaca gtttgcactc ttaaacaacg
    19021 agccatcttt ccatctctct ccctctctct tgtatagatg tgtatgtgta tatctgcatc
    19081 ggtgaatgcc ggtgcttgtg catctatgtg tgcaagtgtg tgcacgccgg aggtcaatgt
    19141 tggatgtctt tatcgctctc tgtcttacta cctagtggac tcttcttcct gacggttgag
    19201 cctacagcat aaggtctgtg agataagagt ctgtactgag gtacagctcc agccctcacc
    19261 ccactttttg aaacagtcac tcactaagcc tggagctcat cagtttggct tagggatcca
    19321 tctccacagt gctgaggtta cagaaagaag cacacataaa acttttatat gggtcctaga
    19381 gatttggact caggtcccat gcttgcataa caagtgcttt ccctgctgag ccctctcctg
    19441 agccctcctg tttttctttg taccacactg ttcattgtca caactcacaa gaacaaggat
    19501 ttcctagagc tcttaccata aggaggatta gaacttcata gcactttaga gtgggatgca
    19561 ggattcagcg ccctcctggg accctgccat gtcccttaac tgacctcctg gatacgcttg
    19621 caaggatatg atgatgggtc atgtaagacc tggttcaagg gctcttgtca cttctgccat
    19681 gtctcgtcta tggcctaggt atgactcaga gccagagaga ggctgaagga gctagtcagc
    19741 gtatcctttg tgggttgttc tgtttgtctt ttgtctatcc ttgcttttct gggtctttat
    19801 agaggcctgg atctcagctg ttctgaggga gtggtggtga gcatctctcc ctccccagag
    19861 tcccatggct agtcagagtc aatgtcacag gaccaggctg acagcacaat gagctttctg
    19921 tcttctggac tcctccttcc caccaggttc ttcatcactg cggttctttc cagagcccag
    19981 tctgccttgg cttgtggttc ttggcttcct ctgtccttgg ttcctacaca acctttccag
    20041 aattagaaac aacctgttgt tgggagcatg tgaggaaagg gatgggttga gagtcaaaaa
    20101 tgtgggatac taaatacagg ccaga
//