LOCUS       KO-first_condition_ready_37498:119413_MGI:102759        22453 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Prss2 targeting project(s): 37498:119413
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 213116
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     exon            8870..8926
                     /db_xref="ENSEMBL:ENSMUSE00000801933"
                     /rank="1"
                     /note="ENSMUSE00000801933"
     misc_feature    4112..9477
                     /note="5 arm"
     misc_feature    9505..9529
                     /note="B1 Gateway"
     misc_feature    9542..9589
                     /note="Frt"
     intron          9590..10464
                     /note="mouse En2 intron"
     misc_feature    10462..10464
                     /note="Splice Acceptor"
     exon            10465..10637
                     /note="mouse En2 exon"
     misc_feature    10649..11242
                     /note="ECMV IRES"
     gene            11243..14303
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14342..14585
                     /note="SV40 polyadenylation site"
     misc_feature    14589..14622
                     /note="loxP"
     promoter        14649..15168
                     /note="human beta actin promoter"
     gene            15194..15988
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16188..16429
                     /note="SV40 polyadenylation site"
     misc_feature    16446..16493
                     /note="Frt"
     misc_feature    16500..16533
                     /note="loxP"
     misc_feature    10479..10556
                     /note="FCHK std_gpr"
     misc_feature    16549..16569
                     /note="B2 Gateway"
     misc_feature    9478..16599
                     /note="Synthetic Cassette"
     exon            16998..17157
                     /db_xref="ENSEMBL:ENSMUSE00000193522"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000193522"
     exon            18036..18289
                     /db_xref="ENSEMBL:ENSMUSE00001023826"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 2 ENSMUSE00001023826"
     exon            18634..18770
                     /db_xref="ENSEMBL:ENSMUSE00001047906"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 3 ENSMUSE00001047906"
     exon            19054..19260
                     /db_xref="ENSEMBL:ENSMUSE00000488689"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 4 ENSMUSE00000488689"
     misc_feature    16600..19314
                     /note="Critical Region"
     misc_feature    19338..19371
                     /note="Downstream LoxP"
     misc_feature    19315..19394
                     /note="synthetic loxP region"
     misc_feature    19395..22453
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg acttctatat gacactagtg aatttgagat
     4141 tctaatttta aggaaaacag taatcatcat tttagtggtt tatacaagat atctttcatt
     4201 ttgccttgct ttcttgattt tagctttctc ctttcttaag gaagtgtgta tccttctctc
     4261 tgagattgtg ttcattttag gagttcatca actcaatata ttgtgatctt tgacttaagg
     4321 tctgatttta ttactgtagc taagaattta caatagttgc aagtcaatct gaaaatgaaa
     4381 attctttctc tgtatatctc agagagcatt cagagagaca atcttctcaa ggattttgaa
     4441 tttaaggaga atatagttta cagagtgaga tacttttaaa aatactgaag atacatgtaa
     4501 aaataatata ggcacaaagt gaaggagaga ggaaagaaac agagacacaa aagaagtgac
     4561 attaaataaa acaaaagaat tatataacta ctggcagagg acagctgaca gttaggcaag
     4621 agcaagctct ctccaggcct gtgaactaac cctacctggt aggttcccat gtctcaatgg
     4681 catgttctac ccaggtgtat gtggggagca ctaattggat atggtgcctc tttgtaggag
     4741 gaggggggaa agacagaaaa aggaagagaa ggaagaaaaa tggagttagt ggaagaaaat
     4801 ggtaaaggag ttgagaagta ttggaatgtt ggacataggc atggaaacta tcaaaatatg
     4861 agtgcacatg attctcaaaa atacatattt taaaatgtaa cttataagaa atatagacaa
     4921 tacaagagga acaagagaat aaaagtgcaa gagatttaaa tgcaaatata aaataggggg
     4981 gaaatgtgtc cttggagtca gatgaaaata agaataacta accccaaatg taaaatactt
     5041 tcaaataaaa taataacaaa aaaaaaaagt tagagaaaag gtaaaggaga tgttgaggat
     5101 gctgggtcct ctactactgt agcttattcg aaggagagta cttccagtct tagctggagt
     5161 cttgtccttc tctgttttta tctcataggt aggaacaact tgtgggatga gggctagctg
     5221 ctcaaatgaa tgctgccagt gtcgttcaca ttagaaactt cctcattttg catgcctgga
     5281 tcatgtccta acctcagtga gctgcactgt gtgggctgga agtgacctca ttcttttcag
     5341 aggctctctg atttttcatt cctaagtaca caagcatcta agctgaggcg tgtgtggtag
     5401 gtgtgtggaa gagctgagga ggcgggagaa gcattcacat ccgtgctggt agcacagaag
     5461 gatccgggta tgcttctggc cattttcaat cttctcagat tcttctcagc agcaaatcat
     5521 tctatgggat ggaggaggct gaggaactgg gatatccaca agttctgaac tcagaactct
     5581 tggtcttacc tgaaagaaat gcagcagaga cgcagcaaca gaggcaggtg gctacctgca
     5641 atttcagaat tctcagtgat cccaataata tagagcccaa agatttgata tttgccatgg
     5701 tgagaggcct caaacagaat cactttccaa agccccagtt tcctaactgc ttaagctgct
     5761 aaccaagagt caccaggccc cagcctcctg tatgaatgac tctcatagtc gcctcttatg
     5821 ctggcaatac agcaaagtat tccagcctcg ctgacgtatg acattttcct ttgttttcaa
     5881 attctcaaaa tggctcatac tcaaataaca ggccccttct caagacggtg tctggctgaa
     5941 tactctcaga gataggaccc tgatattttc acccagactc acctttctta ttctgacttt
     6001 tcttctgcat atgtgtcaca gacaaaactt gtaatatatt gtcttatctg ggatttccaa
     6061 tctatccttt atagtttaaa ttcataattg ctagagattt ctctattcac tttagtatag
     6121 agatttctct attcacttta gtatagtgaa tgaaacccga gaataaatac aacatgcaaa
     6181 ttgctgggca tgaggtttac atgttattta taaatacaaa tgagtagtga aagagagtgc
     6241 ataaatagga tgatctactc caactagctg atcaccaaca actaaactaa gactccaagc
     6301 ctctgtctca aactttagaa attttgtgtg tgtgtgtgtg catatgcatg tgtctgtctg
     6361 tctgtgtgtc tgtctgtctg tttttggtaa ctctttacta accaaagtta gtgaatctag
     6421 gacagcatac tcaggaaaca aagttcctta ggtaagtgaa tttctgctgg agaagagagc
     6481 aagaatggag aaaaggagtt gagatctaca ggaacgaaag cctggaattt catacttgac
     6541 ttaaagagta gtccatagaa ctggggctga agaatgagct aaggaattta gacgcctttc
     6601 atactaaaaa gacaacagaa agactccatt ctgtagtgat gtgttaagtg aatgcttacc
     6661 ttcaagtctg aggtacagaa atgcttatgt ccttaaatcg cagagtgaca aggaccaatc
     6721 caaaactcct cttagcagac atcacttttg tagatattga tgatgtcctc actgtggggt
     6781 gactctcggg gcacagggaa gtgagcagac aagaaagcaa aagttttaca tgcaaagcag
     6841 caatctcgca aatactcccc aggaagcccc atctcaacta catagcagaa agctttcata
     6901 ccacacatag taaaaccctc agactctatg atctctgtga gtgcagagac ttgtgcaact
     6961 gtgtctggaa tggacctggt aggagtgcat ggcaagagta ggaactgaga caatatacaa
     7021 gtgaaatgca aattccgtga tcacgttatg ccaggaagac aaatgccaag aggaaacagg
     7081 atgtttaatt tgtttcacga tgacctctta tttgacagtc acctgcagca aatctcacac
     7141 tctgacaatt cccacatttt tgtagagaca taggggtagc atgccaggtt tagattaatt
     7201 tcttgggtaa aatgaatcca tttttctgaa gacctcatat taaaacattt gtgaaaagtg
     7261 aaaattctga aagtttcaag cactttttag taggacagac cttccactta ggtataagct
     7321 caaaagcctt tatatgagat gcaagatgca gaaatgagag gattcagtac agggactttt
     7381 tgtaaatctg ccctaataat gtcaggaatg gggccactga agagcctgtt gagccccatg
     7441 ttggcctttc ctgcagtata ctctgcattt ccccaacctc tggtcatctt accgtctcca
     7501 ccacaattac agtttaagct cagttttttt cacctttatt tagtatctct agaatgttca
     7561 gtatcttgca aaaaacactg acacataatc tcagctccaa ttgaaagctc ccaaagccta
     7621 gatttggagt aaagaaaaag ataaacaatt gacagcaagg ctcctccaac tgactgtata
     7681 actagattaa aattgatcca tcgccagtca actgtttatc tttttcttca ctttttcttc
     7741 tctggccagg ttttactgca ataaattgag ggatgagcat attataatac tctttttttt
     7801 aaattttatt ctctttttac agtccagact tttatacccc tcccagtcca ccttctgact
     7861 gttccacatc ccatacctct tcccccatct ccaagaggat gtccccaacc cccactccca
     7921 ctcaccagac ctctttttat gttaaaaata gacatttaca gaatcctttc atatctatac
     7981 acaactaatt atctcactgg gacccagatt aaacactgtc caaacatttt ctcacatcca
     8041 aaacatcata tatactttta tctcaggatg cattttccta gaatacgagg tattaatact
     8101 tagtgtaatt ttctggagcc ttgatgaccc ttaaaagtga catatgacaa gagcaaaagg
     8161 caacagaaaa atagagcaca gttttgatgt gagcattcca cccctgtaac atatagagga
     8221 agtacttatt taagttttat ccatcctctt caaaacacac cttcaatcca tgagtctggt
     8281 tagaaaagag ctaagtgaaa gccccagctt agattgaatt ctgcacctcc tagtttttca
     8341 tcatggctgt tttaattgca gaccttagaa ccctttaatt tcatcttctt gttcagtaaa
     8401 atagtcaggt tcatagataa ggggcttagg caatgctgat ttcctaccat gagggcacca
     8461 agcaacccca ctagttacta gaaccagtcc ttgctcaaag ttccttaaaa cattaatgaa
     8521 gtcaggatgt ctaaggatct tagacagtct ttgctcttcc tcttatcacc taagcctaag
     8581 aaatcccact gtggagacaa gggcttgact tcccctggtt tttccattgc atgatccttc
     8641 caagaagcaa ttgtcctcct tatgtgacct gagaaaaaag ttcccttgtc acctgtagct
     8701 cgccatgtga caagggcacc aaacagagca aagctgatgc aagactctgg gaaagagaaa
     8761 cctgcagcta tgtttgtgca gggaaaaaaa aaaatgaccc ctccactttt agccaaggtg
     8821 ccttcctctt tgtttaggga actataaaga caggcactct ttctgtgctc atgactttct
     8881 gtcaccatga gtgcacttct gatcctagcc cttgtgggag ctgctggtga gtttcactct
     8941 ttttcccaag ctctgtctgt tccactgaat gaaatatatg aactatccca atgctgtctc
     9001 ttacctatat tctgatagtc tcaccagcct ctcaaacctt ctccctactt tcctggcttc
     9061 tcattccttg tttttctcac ttagccttta atctactctt gtctccagtc ttcattctaa
     9121 ttggtgccaa gagcagggaa tgttgaaagg tgaagctaac aagaaggttc cataaaatga
     9181 gcctgcatgt ccactgcttg actccagcag ctctgtgata cttccaccac aggtgttctt
     9241 aaggaaaaaa tgtgtttaca gacacaaaaa gcttattttt gctaaatatt caaccattag
     9301 aattctgatc ctatatgtct taggtcttgc ctgtttaata ggtccatctc taaataaact
     9361 tcatcttacc agtcaaagtt aagaaatact gatatattgg caagttcatc ggctatgttt
     9421 cccttgataa gtctaagttg tcttgcatat tcctttctat tcttgattca ttctaaaaag
     9481 gcgcataacg ataccacgat atcaacaagt ttgtacaaaa aagcaggctg gcgccggaac
     9541 cgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcg aaccctttcc
     9601 cacaccaccc tccacacttg ccccaaacac tgccaactat gtaggaggaa ggggttggga
     9661 ctaacagaag aacccgttgt ggggaagctg ttgggagggt cactttatgt tcttgcccaa
     9721 ggtcagttgg gtggcctgct tctgatgagg tggtcccaag gtctggggta gaaggtgaga
     9781 gggacaggcc accaaggtca gccccccccc cctatcccat aggagccagg tccctctcct
     9841 ggacaggaag actgaagggg agatgccaga gactcagtga agcctggggt accctattgg
     9901 agtccttcaa ggaaacaaac ttggcctcac caggcctcag ccttggctcc tcctgggaac
     9961 tctactgccc ttgggatccc cttgtagttg tgggttacat aggaaggggg acgggattcc
    10021 ccttgactgg ctagcctact cttttcttca gtcttctcca tctcctctca cctgtctctc
    10081 gaccctttcc ctaggataga cttggaaaaa gataagggga gaaaacaaat gcaaacgagg
    10141 ccagaaagat tttggctggg cattccttcc gctagctttt attgggatcc cctagtttgt
    10201 gataggcctt ttagctacat ctgccaatcc atctcatttt cacacacaca cacaccactt
    10261 tccttctggt cagtgggcac atgtccagcc tcaagtttat atcaccaccc ccaatgccca
    10321 acacttgtat ggccttgggc gggtcatccc cccccccacc cccagtatct gcaacctcaa
    10381 gctagcttgg gtgcgttggt tgtggataag tagctagact ccagcaacca gtaacctctg
    10441 ccctttctcc tccatgacaa ccaggtccca ggtcccgaaa accaaagaag aagaacccta
    10501 acaaagagga caagcggcct cgcacagcct tcactgctga gcagctccag aggctcaagg
    10561 ctgagtttca gaccaacagg tacctgacag agcagcggcg ccagagtctg gcacaggagc
    10621 tcggtacccg gaagatctgg actctagaga attccgcccc tctccctccc ccccccctaa
    10681 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
    10741 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
    10801 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
    10861 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
    10921 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
    10981 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
    11041 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
    11101 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
    11161 gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
    11221 cgatgataag cttgccacaa ccatggaaga tcccgtcgtt ttacaacgtc gtgactggga
    11281 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    11341 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    11401 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
    11461 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
    11521 gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga
    11581 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
    11641 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
    11701 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
    11761 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct
    11821 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
    11881 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
    11941 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
    12001 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
    12061 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
    12121 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
    12181 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
    12241 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
    12301 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
    12361 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
    12421 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
    12481 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
    12541 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
    12601 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc
    12661 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
    12721 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
    12781 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
    12841 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg
    12901 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
    12961 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa
    13021 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
    13081 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
    13141 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc
    13201 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa
    13261 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac
    13321 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg
    13381 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat
    13441 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca
    13501 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct
    13561 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga
    13621 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca
    13681 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac
    13741 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg
    13801 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc
    13861 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg
    13921 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg
    13981 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct
    14041 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt
    14101 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca
    14161 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga
    14221 ctcctggagc ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca
    14281 gttggtctgg tgtcaaaaat aataataacc gggcaggggg gatctaagct ctagataagt
    14341 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
    14401 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    14461 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    14521 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    14581 gatccggaat aacttcgtat agcatacatt atacgaagtt atgtttaaac ggcgcgcccc
    14641 ggaattcgcc ttctgcagga gcgtacagaa cccagggccc tggcacccgt gcagaccctg
    14701 gcccacccca cctgggcgct cagtgcccaa gagatgtcca cacctaggat gtcccgcggt
    14761 gggtgggggg cccgagagac gggcaggccg ggggcaggcc tggccatgcg gggccgaacc
    14821 gggcactgcc cagcgtgggc gcgggggcca cggcgcgcgc ccccagcccc cgggcccagc
    14881 accccaaggc ggccaacgcc aaaactctcc ctcctcctct tcctcaatct cgctctcgct
    14941 cttttttttt ttcgcaaaag gaggggagag ggggtaaaaa aatgctgcac tgtgcggcga
    15001 agccggtgag tgagcggcgc ggggccaatc agcgtgcgcc gttccgaaag ttgcctttta
    15061 tggctcgagc ggccgcggcg gcgccctata aaacccagcg gcgcgacgcg ccaccaccgc
    15121 cgagaccgcg tccgccccgc gagcacagag cctcgccttt gccgatcctc tagagtcgag
    15181 atccgccgcc accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
    15241 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
    15301 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
    15361 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
    15421 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
    15481 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
    15541 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
    15601 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
    15661 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
    15721 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
    15781 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
    15841 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
    15901 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
    15961 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
    16021 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
    16081 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    16141 ctggagttct tcgcccaccc cccggatcta agctctagat aagtaatgat cataatcagc
    16201 catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    16261 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    16321 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    16381 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ggggtaccgc
    16441 gtcgagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcgtcgaga
    16501 taacttcgta tagcatacat tatacgaagt tatgtcgaga tatctagacc cagctttctt
    16561 gtacaaagtg gttgatatct ctatagtcgc agtaggcggc ctaggtaaaa attttctgtg
    16621 agacactggc ccaggtaaaa agccaatatt caggtggaac agataagaaa tacacatata
    16681 tataaataaa gcaagcaatg ggaagaactg acttctaatg ggatccaatt aagtgttttc
    16741 tcaggtaatt cttgctgtcc catcacggat gctcagaaga gatctgggaa cagatctagt
    16801 tctgtgggaa ctatctgtct gtcctacaga acctgatact gggaagccct aaaatctatg
    16861 ttgcctatta gagatagaac gagtgcagct ttcctacact catggtgctc agtaagaaat
    16921 cctaagggtt ttagctgcca tggaacctcc aatacccaga tgcatttgct tattttcatt
    16981 tcctatctcc catgaagttg ctttccctgt ggatgatgat gacaagattg ttggaggata
    17041 cacctgccga gagagttctg tcccctatca ggtgtcccta aatgctggct accacttctg
    17101 tggaggttcc ctcatcaatg accagtgggt ggtgtctgca gctcactgct acaaatagta
    17161 agtgactaac ccctaagtaa gaagcccacg ttctgggaaa tatttagaac acagcacttc
    17221 gtcaagtggt gaagttgtaa ggtgaatggg ggaaagaaag ccatgaggag aagttgctgg
    17281 cagcagttaa ctattgtgag cactgtggaa aatttgatac agaataggaa ggctctcatt
    17341 tcctcaacaa actcataaaa tagcaatgaa tgtgagtcat taaaccaaga aatatttgtt
    17401 ttgttactat acatacttgt gagaaagtca ggcaagaact ttgtacaacc accactagct
    17461 aagaagaaaa atgaactcca acttttcaga aatacagcta aaaattggta aaactcaatg
    17521 cagtctctac taggctttct cagtcccagg tatgtaacat aaatgacagg gacactgact
    17581 cagtacttgt agagttggga acaagaatgt aaacttccct caaggttcca tgtcataata
    17641 ctttggacta aacaggccta ggtctcacat aataatagtc ctgaataaag caagaaatat
    17701 tactcataga tccttctgtc attaacaaag actcagaaag tgagatgagc atcaaatgtt
    17761 atctcgggtc cacatctctg aatgtgtgag ggactgtggg atggcagatt gaaaaccatc
    17821 cccaaactgt actctttata taccaccaat ctctgaagaa gaagaaaaaa aaaggcaagg
    17881 ttgcttaacc agaaactatc tacccccaaa cagcttctcc tttcacatta cctcaagatc
    17941 tactctagac ctccagagcc actcctgagc aagaagcttg ggaaccctga ggtgtcttgg
    18001 acaaaaacct ctagcatgcg ttttccttcc aacagccgca tccaagtgag actgggagag
    18061 cacaacatca atgtcctgga gggcaatgag cagtttgttg attctgccaa gatcatccgg
    18121 caccccaatt ataattcatg gaccctggac aatgacatca tgctgatcaa actggcttcc
    18181 cctgtgaccc tcaatgccag agtggcctct gtacctctgc ccagctcctg tgcacctgca
    18241 ggcactcagt gcctcatctc tggctggggc aacaccctca gcaatggtgg tgagtaggac
    18301 atttcaccta ctacattcct tccttcccat gttttccaaa accaggcatc tatctggtat
    18361 tgaacctgct ggcttatacc tggcaaataa gtttaaagta ccaattataa aaactgtagt
    18421 tgatacctaa gggagatgtg taacaggatc aacaatgaaa acaggatcaa caagggtcat
    18481 tttacagatt tagaaagcta caattctttg ctggaattta tcttcttaat gatctctgtg
    18541 gtggctaccc atgacattct aggataaact cttgttcctc caagtgcgct gatgaatatt
    18601 tcttctttct ttgatcatta cccttcctct cagtgaacaa cccagacctg ctccagtgtg
    18661 ttgatgcccc agtgctgcct caggctgact gtgaggcctc ctaccctggg gacatcacca
    18721 acaacatgat ctgtgttggc ttcctggagg gaggcaaaga ttcctgccag gtaagtgact
    18781 ctcattggat atgaaacctc tgttctcctg ggagtggcat gtgagtgtcc attattggga
    18841 aacttgaagc ttcacacagt gggatcaggc aggtatgaga tctatgctga atgtcatttc
    18901 atctcattag tgagaataga caatgttgtg tttcaatgca gaaaaaaaaa atggaactat
    18961 tctagagtaa atagaatgag gtcttaaggt cagagtaaat gtgattcttt ttccacatat
    19021 cccttcccta aacactgtat ttctctaatc cagggtgact ctggtggccc tgtggtctgc
    19081 aatggagagc tgcagggcat tgtctcctgg ggctatggct gtgcccagcc agatgctcct
    19141 ggtgtataca ccaaggtctg caactacgtg gactggattc agaacacaat tgctgacaac
    19201 tagagaaccc tagtctctct tcaatcagta ttatcaataa agttcatttg tcatcactgt
    19261 atgtttgtct ctttcacctc tctagtccct tagctctggg aagcatgctc ttatgagatg
    19321 gcgcaacgca attaatgata acttcgtata gcatacatta tacgaagtta tggtctgagc
    19381 tcgccatcag ttcactggat agcaaaatag aattcattaa ataatcaaca gcagcatagg
    19441 aaacacaaaa ataatttcag ggtgtatgat aaaggtacat gcttttagtt ccagtacttg
    19501 agaggcagtg gcaggctaat ttctgagttc aaggccaacc tggtctacag agtgagttct
    19561 aggacagcca gggatataca gagaaatcct gtctcaaaaa tcaaaattaa taataattaa
    19621 taataataaa tagtaaattt ctgtagaatc ataatgagga gcttgtttca ttttactttt
    19681 tcaagaaatg ttgtgtctga atagtggacc aaagcttctc ctttttgttt aagatttatt
    19741 tatttaatgt atatgagtac actgtagctt tcttcagacg catcagaaga gggcatcgga
    19801 ccccagtaca gatagtttga gccaccatgt ggttgctgag aattgaactc agaacctctg
    19861 gaacagcaat tagtgccctt aaccactgag ccatctctcc agcccccaat gcttttcttc
    19921 cttagaattt caatggggct gaaagtagta atttgtgaat accttgtggt tgaggatact
    19981 actgatttct ttacatcaga aagtcttcat gttcaatata aatcttagct atggttaacc
    20041 tccttggctt ccgagttcca tccttcatat tcattttggc agctttgatg caaagtcttg
    20101 ctttgtaatt caagtaggcc ttgaacttgt aattttcttg cttcaggctg cagaattctt
    20161 agactgtggg ttgcaccact atggctggct ctctacgtaa aaatgtttat ggcaatgcag
    20221 tcctactctg tcctaacagt tgggttactt ggagaaggtc ttactgttca tcatcaaatt
    20281 cctacttatg tttctataga agaaataaga ggttcttggt ctacaaccgt cataactcta
    20341 agcaattccc ctctgtttat cttacacagt aatactaata tgtatggacc accttgagaa
    20401 tttcatagac actttcacat ttacttcatt tttcccttct gtaatgatta ttccagttcc
    20461 acagctagaa agcaaagact cagagaaggt tgttcactcg ctgcatccta cacatagcgg
    20521 ctgatagaac tggctattgt cagaatttct tagatatttg catgctcact agtggcatcc
    20581 cttctctttc tactgtgtga tcttagatga gaaagattcc atctaccttg atctttgata
    20641 tacctccaga ctactctcac caacaaacac ccattcctgc ttccatgcac ttccaggaag
    20701 tatctcctag tcactgcctt tcatgaagtc tactcttaga gaattcttgc tgagccagaa
    20761 ctacttctga catcctattt gaacatgagc attaagttgc ctcaacacta aacccaaaac
    20821 cacataaagt tgctctataa cctgatcagt tggagtaaca ggaaagagag tttcaaatac
    20881 ctactcaaca atgggaccac aaggtctcta caccaagaaa cattactatg acataacttc
    20941 agatacatag gtcccattgc aaaaaatgca agcatagaga agccaaaaat taataccccc
    21001 atagaactgt tctctcagaa aagtgacttg gataatgaaa aagacaatga taactacaac
    21061 aaaaattact ataacaaaga tggtcaagga acttaactag gatatgaata agtgccacaa
    21121 tgaagactgt gaaaacacag tcaaattaaa aaaaaaagaa ttcaaaatat gaaactggaa
    21181 tttaatgaag aggtagtatc tctgaagaat agtcaaacaa aataaaacaa tacaaagctt
    21241 caggaattta ccctacccag agaagccagt gagctaactg cagatctgtt gcggtaaatc
    21301 tccaacccaa atgagcctgg gcaatgaaaa cacaactcaa ctaatatgaa tacatgctgt
    21361 gcacctagat tgggcagatc tacactacca tcttgccagt actagagacc ccttataact
    21421 tgcggtttct ccaggccagg tgcttctgct ccactttcct ttttcctcct ccttcattgt
    21481 tcgttctctc tctctctctc tctcccccaa aaccttcatc tcaaccttcc cctcttcctc
    21541 ccccaatcac cggctctagc ctttatttat aaattaaggt ttacaggaaa tcacctgagt
    21601 gctgactcat tcctcattta caacccctca caggagaaca gaattggcat caaatataat
    21661 tagttccggg gctatctgca acacagatct ttatgtctcc tttctctcct ctaaatccaa
    21721 tccttgggcc tcattctcag ctctgtagca gcctcccttc cctgtctgac cccttcttca
    21781 ctttcatcac acagacttcc ccctcctagc ttccttactc tgacctactg ctttacccca
    21841 gcccccaact ctagcaggca ttcctctgga acctgcagct cactcctgct agagaagcaa
    21901 gttcccttaa tggcaaatct tcagctctcc ttcaaaacca attccccaat ctcactccca
    21961 tctctgtagc agaccacaca ctgtggcttt cttctacttt ctgccctcct ctctaacaga
    22021 tcatggagat cttctcctcc aaacatcttt cccattcacc cagttatccc agatcccgac
    22081 tccccaggct tctgtgagat cccatgagca aagcctgcca gagaagccag tgaggcaggt
    22141 aagtgagctt cagaccttca ttctccctcc tctcctgcat gtataatgcc ccagcttcat
    22201 ccccaactcc atagcagact acttattgag acctctactc acattcccag aggactgagg
    22261 aggtccctgt ggaattcctg atttcctctg ttctgaggac cccatcagtc tgctgcaatc
    22321 caaacccatt catacgtgtc aactcccaat acctaggaaa acattttacc tggaagccca
    22381 gcggccacac atggtagaat actggtggaa ttatctacca gacaacccac aactatcttc
    22441 agtttatagc tga
//