LOCUS       KO-first_condition_ready_82517_MGI:1201683        21580 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Eomes targeting project(s): 82517
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 40312
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6816..8018
                     /db_xref="ENSEMBL:ENSMUSE00000490918"
                     /rank="1"
                     /note="ENSMUSE00000490918"
     misc_feature    4275..8483
                     /note="5 arm"
     misc_feature    8511..8535
                     /note="B1 Gateway"
     misc_feature    8548..8595
                     /note="Frt"
     intron          8596..9470
                     /note="mouse En2 intron"
     misc_feature    9468..9470
                     /note="Splice Acceptor"
     exon            9471..9643
                     /note="mouse En2 exon"
     misc_feature    9655..10248
                     /note="ECMV IRES"
     gene            10249..13309
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13348..13591
                     /note="SV40 polyadenylation site"
     misc_feature    13595..13628
                     /note="loxP"
     promoter        13655..14174
                     /note="human beta actin promoter"
     gene            14200..14994
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15194..15435
                     /note="SV40 polyadenylation site"
     misc_feature    15452..15499
                     /note="Frt"
     misc_feature    15506..15539
                     /note="loxP"
     misc_feature    9485..9562
                     /note="FCHK std_gpr"
     misc_feature    15555..15575
                     /note="B2 Gateway"
     misc_feature    8484..15605
                     /note="Synthetic Cassette"
     exon            15990..16144
                     /db_xref="ENSEMBL:ENSMUSE00000219777"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000219777"
     misc_feature    15606..16323
                     /note="Critical Region"
     misc_feature    16347..16380
                     /note="Downstream LoxP"
     misc_feature    16324..16403
                     /note="synthetic loxP region"
     exon            16771..16892
                     /db_xref="ENSEMBL:ENSMUSE00000219775"
                     /rank="3"
                     /note="ENSMUSE00000219775"
     exon            17495..17653
                     /db_xref="ENSEMBL:ENSMUSE00000219776"
                     /rank="4"
                     /note="ENSMUSE00000219776"
     exon            17739..17800
                     /db_xref="ENSEMBL:ENSMUSE00000219780"
                     /rank="5"
                     /note="ENSMUSE00000219780"
     exon            19780..21580
                     /db_xref="ENSEMBL:ENSMUSE00000373976"
                     /rank="6"
                     /note="ENSMUSE00000373976 5' fragment"
     misc_feature    16404..21580
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggagggact aatgtagtcc agaagtatca gtaataaaat gtaagttaat
     4321 taacataaaa tatttgattt ttttgtctct atgaagctag gaaaaatatg tttagttgaa
     4381 acggtgcttt tcaccatttt aaagggaaag tcttcacgat ctgacagtta cactaatgcg
     4441 tcctaacaac actaatgtta accctaataa acttcccata tctcaaaaat cccaggtccc
     4501 aaaagacttt cctagcgctc ttgtttttga ttctttttgg atttatcttt atttctgtaa
     4561 aatgtgtcag aaatcggatg aaagtgtatt tactgaggtg gggttccagg aatccgttca
     4621 aattctagtt acacagattg aattacaccg agttaataat ttgatggcac tagaaacgaa
     4681 cctatgcgcc ttccttcatg agcttgctct aggggtagga tagaatgaac ccgattctga
     4741 tgtggttagg gagacagctg gtctgagtta cttgcgccag agtgggtctg aacggtgccc
     4801 gttgcctgtg tggcactgtg ccacgccagc gtttccccgg tctcttcaaa gtattttgaa
     4861 gggaccttac ttctggctgt gttataatac aaaactgaca tataacttca gtcaccccat
     4921 ctgcagataa cgtgagattc agtctcacac ctacatctcc ctcagacatc ctgcagctca
     4981 gggccaggct aacgaagact ccaggttgag tccccagcgc agtgaggccc ttttcttgga
     5041 actcgcatcc cccttgtgga atcctcacgt tcaatatctc gcaccctttg aggggaataa
     5101 aaattaatcc caaaggcttc gctgcatcaa aacaaaacaa aaacatgcgg gacggagccc
     5161 gagaccaaat cggagctggg atctctggac tgatcgcgca ggatgtcagc ccgagttctc
     5221 tgagccaaaa ttgacaaggt tgttgttcca aaacacttga aaagctttgc gagcgcccaa
     5281 gacccactac gctattctat caaaacccca cacagcatcc aagtccatga aggatcgctc
     5341 cactccctaa cacgggcgtg cccacgttcg tgattcgcgg ggtttggacc caggttggaa
     5401 agcttcgcgc ggatctgctg ctggcgtctg agcgcttagc agatcccgcg gccacctcat
     5461 ctgttctcgg caaaagtttc ccgtgtgatc gcattgggtt gggggaagca gagtccctac
     5521 atctcgaccg gaaaatgcgc tcctggagcg actactgcag gcgtccgtaa ttgcttatta
     5581 gaggagaatg tacaggcttc tcatcgtcaa cgaaacgtgt tcccccttct gtaataataa
     5641 aacaataaaa tatgacggcc ccgtccttga ctgtttgcgg aaacgcaggg gggaaaaaca
     5701 ggcagctgga gcaaaggagc ttaattcaat atcaaactga ttatttcact aattattctg
     5761 ccttctgtat tgtgccgcag agaggcgcag ggaatcttaa ctgcacagct gctagggaac
     5821 ctttgtgtct tagtgtcagt ccccaccaaa gatgttggcg aaagcggggg ctgggggagg
     5881 taggaaacat ccagtgttta gaaaaaaccg agcagaggga attctgagta atgaaagtga
     5941 cggcctcggg gaagccggtg ttttagcgcg gttgtaatac tgagtgtttg gttcgtccgg
     6001 tcaggctcat gttgggtctt cagaagccca cttcagaagc tagaagctgg agtctctgat
     6061 tcagacgcgg aatccttttg ccatctctgg aatcccaaaa actgaacgcg aggtttctct
     6121 ccaccaagtc tttacgctaa aagatgggac aggacaaaaa gcggttcctg tgtgctgaag
     6181 gccaggaagt cagaaggagg gtgtgcgcag agacttcggg tcgctctcca gccctcccct
     6241 agatttgcac ccccggggac ctgagccttc ctgcgagcag ggagcttgta aagacgcctt
     6301 cacgggcctc tgggacctgc caaactagac ccgaactcag gaatgattcc tatgccgtgg
     6361 gtagaccatg ttcgcagact tcaaacccgt ttctccggcg ccatagagcc accgggtaca
     6421 ccaggattga ttctcagtgg gatatagttc tcccaaagac gcttaaagaa acaccaaacc
     6481 agcaataaca gccaaataag ctaagagaaa atgaaactcg caggcgaccc gatccaatta
     6541 ataaatactt aattgaatta gtgtcacctc taacagccaa tagcaaagtc ccctagccat
     6601 ggggctgcga agcccctccc tggccgcaca tatataagca gccaaccacc gctcacaagt
     6661 ttccaagcgg tcaagtatgc ctagagttta gcagagccag ccgagaggaa atggctggtt
     6721 gctaaatggg aactcagagc taagtcctct tcttccttag tgtgagtgta ggggtcctga
     6781 gaggttagac aaggagggaa ggaagaaaag aaggaaggaa ggaaggaagg aaggaaggaa
     6841 ggaaggaagg aaggaaggaa ggaaggagag agacacctac aacccggggt ttgttttctt
     6901 gcggaaggaa aggggcacct acaatcccga gtttgttccc ttgagggaag gacgctaaat
     6961 tgggcgcgcg ctgaagccgc cccgcaggtc cgcaggtggg tagcctaggc gagggaggaa
     7021 ggggacatta ccttcccctc ggaagagggc gctggctccc ccaactggcc tttataacca
     7081 ggccgcggga ggcgaggagg cagccggttg ccgtctgcga ttcgctaaag catgcagttg
     7141 ggagagcagc tcctggtgag ctcggtgaac ctgcccggcg cgcacttcta ctcgctggag
     7201 agtgctcgcg gaggaggagg aggaggaggc ggaggaggag ggggaggcgg agggagcgtc
     7261 agcctcctcc ccggtgctgc cccctcgccc cagaggctgg acttagacaa agcgtccaag
     7321 aagtttccgg gcagtctccc gtgccaggcg gggagcgcag aacccgcagg cgccggcgcg
     7381 ggggcccccg cggccatgct cagtgacgcg gacgctgggg acaccttcgg gagcacctcg
     7441 gcggtggcca agcccgggcc cccggacggc cgcaagggct ccccgtgcgc ggaggaggag
     7501 ctgccctccg ccgccaccgc cgcggccacc gcgcgctact ccatggacag cctgagctcc
     7561 gagcgctact acctcccgtc gccgggaccg cagggctccg agctcgccgc gccctgctcg
     7621 ctcttccagt acccggcggc ggccggagca gcccacggac ccgtgtaccc cgcgtccaat
     7681 ggcgcgcgct acccctacgg ctccatgctg ccccccggtg gattccccgc cgccgtgtgc
     7741 ccgcccgcga gggcgcagtt cggccccgct gcgggttcgg ggagcggcgc tggtagcagc
     7801 ggcggtggtg ccggcggtcc tggcgcctat ccctacggcc agggttctcc gctctacggg
     7861 ccatacgccg gaacctcagc ggccgggtct tgtggaggat tggggggcct aggggtgcct
     7921 ggctccggct tccgcgccca cgtctacctg tgcaaccggc ccctatggct caaattccac
     7981 cggcaccaaa ctgagatgat catcaccaaa cagggcaggt gagtgcaggg tggagggacc
     8041 ccaggcgcct ggttgtccgc acactgcggg ctctgcttag ggtgtttaag tggtacatca
     8101 tatctccaaa gtcgtgtccc ttacttccta aggcacacct gtagcttggg ctccgagccc
     8161 acgttactct cctctctagt caccttcacc gggattgact gggaggagga ggaggcggag
     8221 atggaaattt gggaatgaac ccttgagggc agatctacgt gacccgctgg ctcctgggtt
     8281 tgcagcaggc gatttgtggg ctgcatagcg aaagagggct agctggagct ggctgcggcc
     8341 tgcgcacaag tgtttgaaaa gaagcttttt aaagctcctt ggtttggtgg ggctcttctg
     8401 taagaagagc agaaagaagt cggtttgagc tggtgactag gtagacagtt tttggagcca
     8461 gatgtttggg ggagggggct ctgaaggcgc ataacgatac cacgatatca acaagtttgt
     8521 acaaaaaagc aggctggcgc cggaaccgaa gttcctattc cgaagttcct attctctaga
     8581 aagtatagga acttcgaacc ctttcccaca ccaccctcca cacttgcccc aaacactgcc
     8641 aactatgtag gaggaagggg ttgggactaa cagaagaacc cgttgtgggg aagctgttgg
     8701 gagggtcact ttatgttctt gcccaaggtc agttgggtgg cctgcttctg atgaggtggt
     8761 cccaaggtct ggggtagaag gtgagaggga caggccacca aggtcagccc ccccccccta
     8821 tcccatagga gccaggtccc tctcctggac aggaagactg aaggggagat gccagagact
     8881 cagtgaagcc tggggtaccc tattggagtc cttcaaggaa acaaacttgg cctcaccagg
     8941 cctcagcctt ggctcctcct gggaactcta ctgcccttgg gatccccttg tagttgtggg
     9001 ttacatagga agggggacgg gattcccctt gactggctag cctactcttt tcttcagtct
     9061 tctccatctc ctctcacctg tctctcgacc ctttccctag gatagacttg gaaaaagata
     9121 aggggagaaa acaaatgcaa acgaggccag aaagattttg gctgggcatt ccttccgcta
     9181 gcttttattg ggatccccta gtttgtgata ggccttttag ctacatctgc caatccatct
     9241 cattttcaca cacacacaca ccactttcct tctggtcagt gggcacatgt ccagcctcaa
     9301 gtttatatca ccacccccaa tgcccaacac ttgtatggcc ttgggcgggt catccccccc
     9361 cccaccccca gtatctgcaa cctcaagcta gcttgggtgc gttggttgtg gataagtagc
     9421 tagactccag caaccagtaa cctctgccct ttctcctcca tgacaaccag gtcccaggtc
     9481 ccgaaaacca aagaagaaga accctaacaa agaggacaag cggcctcgca cagccttcac
     9541 tgctgagcag ctccagaggc tcaaggctga gtttcagacc aacaggtacc tgacagagca
     9601 gcggcgccag agtctggcac aggagctcgg tacccggaag atctggactc tagagaattc
     9661 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     9721 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     9781 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     9841 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     9901 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     9961 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
    10021 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
    10081 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
    10141 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg
    10201 gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccat ggaagatccc
    10261 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    10321 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    10381 caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
    10441 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca
    10501 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg
    10561 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
    10621 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg
    10681 gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg
    10741 tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg
    10801 ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt
    10861 ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc
    10921 actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc
    10981 gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc
    11041 agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc
    11101 gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc
    11161 tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc
    11221 gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg
    11281 ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat
    11341 gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc
    11401 tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat
    11461 gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat
    11521 gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt
    11581 aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac
    11641 gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc
    11701 ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa
    11761 gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga
    11821 gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt
    11881 ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg
    11941 gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac
    12001 ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt
    12061 gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag
    12121 ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat
    12181 aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg
    12241 cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg
    12301 gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg
    12361 tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg
    12421 acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc
    12481 atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg
    12541 tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg
    12601 ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa
    12661 cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat
    12721 acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc
    12781 ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt
    12841 gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg
    12901 gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac
    12961 cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg
    13021 tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca
    13081 caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg
    13141 gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt
    13201 ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag
    13261 ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc
    13321 aggggggatc taagctctag ataagtaatg atcataatca gccatatcac atctgtagag
    13381 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    13441 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    13501 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    13561 ctcatcaatg tatcttatca tgtctggatc cggaataact tcgtatagca tacattatac
    13621 gaagttatgt ttaaacggcg cgccccggaa ttcgccttct gcaggagcgt acagaaccca
    13681 gggccctggc acccgtgcag accctggccc accccacctg ggcgctcagt gcccaagaga
    13741 tgtccacacc taggatgtcc cgcggtgggt ggggggcccg agagacgggc aggccggggg
    13801 caggcctggc catgcggggc cgaaccgggc actgcccagc gtgggcgcgg gggccacggc
    13861 gcgcgccccc agcccccggg cccagcaccc caaggcggcc aacgccaaaa ctctccctcc
    13921 tcctcttcct caatctcgct ctcgctcttt ttttttttcg caaaaggagg ggagaggggg
    13981 taaaaaaatg ctgcactgtg cggcgaagcc ggtgagtgag cggcgcgggg ccaatcagcg
    14041 tgcgccgttc cgaaagttgc cttttatggc tcgagcggcc gcggcggcgc cctataaaac
    14101 ccagcggcgc gacgcgccac caccgccgag accgcgtccg ccccgcgagc acagagcctc
    14161 gcctttgccg atcctctaga gtcgagatcc gccgccacca tgattgaaca agatggattg
    14221 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
    14281 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
    14341 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
    14401 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
    14461 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
    14521 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
    14581 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
    14641 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
    14701 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
    14761 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
    14821 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
    14881 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
    14941 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
    15001 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
    15061 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    15121 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccccg gatctaagct
    15181 ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa
    15241 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
    15301 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    15361 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    15421 atcatgtctg gatccggggg taccgcgtcg agaagttcct attccgaagt tcctattctc
    15481 tagaaagtat aggaacttcg tcgagataac ttcgtatagc atacattata cgaagttatg
    15541 tcgagatatc tagacccagc tttcttgtac aaagtggttg atatctctat agtcgcagta
    15601 ggcgggagtg gatattggga gacagggacc caaagagggc tcgttgagag agagagagag
    15661 agagagagag agagagagtg caagaaggcg cgctccggcc taatgttctc agtcacctat
    15721 gtctttaaaa ataattttag acatttcaga aagaaaccta gtagatacct gaaaatagat
    15781 ggatgtctca gaaaagaagc acgcgaatta gggaaaatac tgatttggag gctgttccaa
    15841 ataaatatgt caatttaaaa ccattttctt agtttcaggc cgtagtagcc atttttgttt
    15901 gtttgctgtt tattgcagac cccccatgtg tccgggaggg accagagccg ctacttaaat
    15961 aatttcctct ggttctgttc tttgcacagg cgcatgtttc ctttcttgag cttcaacata
    16021 aacggactca accccaccgc ccactacaat gttttcgtgg aagtggttct ggccgaccct
    16081 aaccactggc gcttccaggg gggcaagtgg gtgacctgcg gcaaagcgga caataacatg
    16141 cagggtgagg agagaggggg cctggaccgg gaagaggtcg gagcctcagt gttggaccgg
    16201 ccacatttaa atcaagcatg tcaccccaac ctggggcatg gggatgttag catgtgctgc
    16261 cttctgctgc tggggagaaa tttactccgt cctttttctt gtttctcatt atatatcaaa
    16321 cctgagatgg cgcaacgcaa ttaatgataa cttcgtatag catacattat acgaagttat
    16381 ggtctgagct cgccatcagt tcaaattctc ttgagaaagc ccaggccacc tttaacttaa
    16441 tctataaaag ttaaggactc gatgaggcac ttcccctcca gcttaaggtc tcagaccaac
    16501 aatcaagaac catcctctct cctctcaact ctgtttttta agtttggaga ggctggtgat
    16561 tggaatacca tgattaagga aggctggatg caccctggcc cgggggagtg actggagtcc
    16621 cgctgggatc cactgatttc agtcgactga cctcccagga ggaggttgct acagcaaacc
    16681 caagcctagc taacagacat cctctccaac ctgcctgggt gaaggctgtc tcctttcatt
    16741 tgcttatgtt tcctttcctt ttcctcttag gcaataagat gtacgttcac ccagaatctc
    16801 ctaacactgg ctcccactgg atgaggcagg agatttcctt tgggaagtta aaactcacca
    16861 ataacaaagg tgcaaacaac aacaacacac aggtagggag actggaaaag cctgtgtctt
    16921 ctggtcgggg tctcttggac agctcatcct tttgtcactg tgcttagcta agtatttaca
    16981 gttgtgactt tgctcaattt taaatttatg tatttacctg tgtcatttgt actttcttac
    17041 tactatgcat agaaacatca aattttaggg ggggacttgg ggagccaaaa ggaaagagta
    17101 tctatcaaat acacagacac tctgtttttg ttttggttgt tgtttgtttg tttgtttgtt
    17161 tgtttcttat cttggggagt gggtcttgta tacctagctc gggctggcct tgaactgctg
    17221 accctcttac ttctcagtct cagatgctgc ggttacagat gtgctctgac attccagaat
    17281 cagagttagg actttaagtc gaaattgtta atcccgagga tcttaaatct taaaacagaa
    17341 tctttaataa aaccaaataa acaaagaaaa tccccctcaa aaacaaaaaa gaaagctgta
    17401 gtagggagca aacagaagtc ctgtctcaaa atgtacagtc tgttaaaaat ctacttttga
    17461 gacctatgaa agtctctctt ttctctatcc ccagatgata gtgttgcagt ctctgcacaa
    17521 ataccaaccg aggctgcaca tcgtggaagt gacagaggac ggtgtggagg acttgaatga
    17581 accttccaag actcagacct tcaccttctc agagacacag ttcatcgctg tgacggccta
    17641 ccaaaacacg gatgtgagtg ccccgtgagc cacaggagcc tctcaagtta ggtctattga
    17701 gataaagcac tcagttacta ttcttctccc ctatctagat cacccagcta aagatcgacc
    17761 ataacccctt cgccaaaggc ttccgggaca actacgattc gtaagtactg cttttcttcg
    17821 agcacgggtc atctccctct aagagacaag agtaaaagac tccaggctgc ctgcaacttg
    17881 ctgtgaagtt gacgtgacct tgaatttagg atctttctgc ctcagtcccc ctagccccga
    17941 gctcaccagc atgggctgcc acacccaggc tttgtgtgag ctagacaatc atttggtcaa
    18001 tggagtttca tcttcgagcc catttgctgg gtcaccttta acaggaaatg agtcatcatc
    18061 tccctgagga catatttagt atgacatttt atatttggga tttttttttt cttacctgtt
    18121 tttctgctta attttctgaa acacacacac atgctggaga ttgaactcag ggctttgtat
    18181 gtgcaagaca agcactctac cattgagcta tagccctagc cctgtttttg catatattgg
    18241 cctcgaacat tcttgcttca attaaaaaaa aaaaaaaaaa aaaaactggt ttagttaaag
    18301 aatgtttaag acttaaacat gaagtttttt tttcttttct gcacttaagt agggatttga
    18361 attattttag aatctccatt acttatgtcc atttgttgtg attataaaaa gataaagaaa
    18421 tataagaata tattctatgg gggtgtggtg acgtgtgggg gaaagggatg tctcagcagg
    18481 ctcatgtaga ggcatcccgg caccctgagg gaccagccac aagatggaat agtatagaat
    18541 agagtttatt tagggcatgg ggagggaggt taagagggta gtagaggcag agagaggaag
    18601 agagtagaga agtagaggcc ggccatgacc acatggagag aggcagggaa gggaatgggg
    18661 agagaggagg aacaagtgag caagaggcaa gagagagagg agggggcaag gagacccttt
    18721 tacagtgggc caggcctacc tggctgttgc caggtaactg tgggggcgga gcttagacgg
    18781 aatgctaacc cctttccctt tttacattta tttacctatt tatttattta ttttttactg
    18841 ttttgagtca ttgtttatcc atgttgagtc agtgtgtagc cctggctgtc ctggaactct
    18901 ctctgtagac caggatggcc tagaactcag atccacctgc ctctgccccc gaaagctggg
    18961 atcaaaggtg ggtgctgaca ccttccagcc caatttttat ttttttaaaa aaggttttat
    19021 ttttaattat gtacatttgt gtctctgtgt gggtatgtgc atgtgggtgc cagtaggcca
    19081 aaggtttcag gtcccttaga gcaggagtga caggtggctg tcagtcagct gatgtgtgtg
    19141 cttccaacca aactctagtc ttcctcaaaa gcagttcttg accttccagc tcctgatcca
    19201 acttttaaaa aagcatcact agcagatttt ttccccctcc ttacgtgtca caagagaaaa
    19261 aaaccccgaa aggatactgt ttttcacaac atttgactct cacggccctg cattccgtcc
    19321 gccccgttga ttagggaggg ttgttctagt ccctcgtgtt cgaagactgc tacagtagat
    19381 tttccatatt tatttatttt gagacagggt ctcatgtgat caaactcaaa ccggctctgt
    19441 agctccaatg acctttcacc tctgacctcc agcttccacc tccccagtac cgggattaca
    19501 ggcctcggcc acctctgatt tatgttgagc ttcagagaga cacccaggcc tgtctgtgtc
    19561 aatgctgttg aggctcgcct tcgaccccat gatatagcca cagatcccag tttcccatag
    19621 gaaatatctg acccctggag gaagtgtcct cttaaggggt ggggaatcca ctctgttctc
    19681 acagtcggca gcacaactct ctagcttctg ggaggcagct tgtttgggga caacgtgagg
    19741 gttgtttttt atttttaagt gtctctcttt atattgtagc atgtacacgg cttcagaaaa
    19801 tgacaggtta actccatctc ccacggattc ccctagatcc catcagattg tccctggagg
    19861 tcggtacggc gttcaaaact tcttcccgga gccctttgtc aacactttgc ctcaagcccg
    19921 atattataac ggtgagagaa ccgtgccaca gaccaacggc ctcctctcac cccaacagag
    19981 cgaagaggtg gccaaccctc cccagcggtg gcttgtcacg cctgtccagc aacctgtgac
    20041 caacaagcta gacatcggtt cttatgaatc tgaatatact tccagtacct tgctcccata
    20101 tggtattaag tccttgcccc tgcagacatc ccatgccctg gggtattacc ctgacccgac
    20161 cttccctgct atggcagggt ggggaggccg tggcgcttat cagaggaaga tggcagctgg
    20221 actaccatgg acatccagaa tgagcccacc tgtcttccca gaagatcagc ttgccaagga
    20281 aaaagttaaa gaagagatta gttcctcctg gatagagact cccccctcca tcaagtctct
    20341 agactccagc gactccgggg tgtacaacag cgcttgcaag agaaagcgcc tgtctcccag
    20401 cacccccagc aatggaaact cgccccccat aaagtgtgag gacattaaca ctgaagagta
    20461 cagtaaagac acctccaaag gcatgggggc ttattatgct ttttacacaa gtccctagag
    20521 cattatttta acctttaacc tttccagatg gacctggtgg tgttttgttg tgttctttgc
    20581 ctaggttacc aaaaagactt ttgccttcca cctttgatgt atcctgtttt gtgcaattct
    20641 ctgagagaag gcgccaaagc tttgccacgg ccgcaggtaa ctaaactgaa gcagacctag
    20701 cattggtttt aaagtgactg gaggacgcag gagtgttttt aaaactgaaa aggtcgttca
    20761 aggtgctgga ttgattcatt tatgggaaac gagaaatgtt cagaaaaagc aggctatgaa
    20821 gaacgagtgc ccggtgctat taaatgaatt tcaaagacaa gaaaacatga cagaaaaaaa
    20881 caaaacaaaa caaactttgg ctcttatttc tactcataaa tctttaagca aacagcagcc
    20941 cagagggtta aaatattttt gcttcgggaa gagaagggtt ggaccttgag ttaacagtgg
    21001 gctcttcaca gatatcttct gccagggcct atagaggctg agcctgagag tcaagcgggg
    21061 aaaacaaaca aacaaacaaa ctgatgttta agtggctagc tagtactcac tgccttcaat
    21121 tccttgcaga attttggaat aaaccatttg ttgtagggct ggctctgtgt ggctgggatg
    21181 acctgtagaa ggcaggagga tcagaaagaa agggagaagt taagtgtaca attgtatgtt
    21241 tagacacctt agaatattct ggaatatatt gtacaaattc aactgcctag gtatcagata
    21301 aaaataatgt aaatttggtg ctttggcgtt gtaaagaatt tgcaaaacca caactgcagt
    21361 taagaggagg gattcatatc ttggagatat tctgtccact tcgctaagta ctcagctgcc
    21421 tccgtacttg cttctacaca cttaaggaaa aaccctgctt aatcctcttt ccttggtcat
    21481 ccccacttcc cccttcagta aatgtgaaaa aaaaaattgc agtatgatgt cacagttcct
    21541 ataggctttt tagagattct gaatttttat gtacagtcat
//