LOCUS       KO-first_condition_ready_67877_MGI:1921812        22326 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Fer1l4 targeting project(s): 67877
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 256493
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            8376..8475
                     /db_xref="ENSEMBL:ENSMUSE00000680967"
                     /rank="4"
                     /note="ENSMUSE00000680967"
     exon            8205..8293
                     /db_xref="ENSEMBL:ENSMUSE00000680968"
                     /rank="3"
                     /note="ENSMUSE00000680968"
     exon            7835..7890
                     /db_xref="ENSEMBL:ENSMUSE00000680969"
                     /rank="2"
                     /note="ENSMUSE00000680969"
     exon            7504..7582
                     /db_xref="ENSEMBL:ENSMUSE00000680970"
                     /rank="1"
                     /note="ENSMUSE00000680970"
     misc_feature    4275..10209
                     /note="5 arm"
     misc_feature    10237..10261
                     /note="B1 Gateway"
     misc_feature    10274..10321
                     /note="Frt"
     intron          10322..11196
                     /note="mouse En2 intron"
     misc_feature    11194..11196
                     /note="Splice Acceptor"
     exon            11197..11369
                     /note="mouse En2 exon"
     misc_feature    11381..11974
                     /note="ECMV IRES"
     gene            11975..15035
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    15074..15317
                     /note="SV40 polyadenylation site"
     misc_feature    15321..15354
                     /note="loxP"
     promoter        15381..15900
                     /note="human beta actin promoter"
     gene            15926..16720
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16920..17161
                     /note="SV40 polyadenylation site"
     misc_feature    17178..17225
                     /note="Frt"
     misc_feature    17232..17265
                     /note="loxP"
     misc_feature    11211..11288
                     /note="FCHK std_gpr"
     misc_feature    17281..17301
                     /note="B2 Gateway"
     misc_feature    10210..17331
                     /note="Synthetic Cassette"
     exon            18045..18086
                     /db_xref="ENSEMBL:ENSMUSE00000680965"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 1 ENSMUSE00000680965"
     exon            17805..17896
                     /db_xref="ENSEMBL:ENSMUSE00000680966"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00000680966"
     misc_feature    17332..18186
                     /note="Critical Region"
     misc_feature    18210..18243
                     /note="Downstream LoxP"
     misc_feature    18187..18266
                     /note="synthetic loxP region"
     exon            21822..22192
                     /db_xref="ENSEMBL:ENSMUSE00000680962"
                     /rank="15"
                     /note="ENSMUSE00000680962"
     exon            21346..21532
                     /db_xref="ENSEMBL:ENSMUSE00000639599"
                     /rank="14"
                     /note="ENSMUSE00000639599"
     exon            20474..20657
                     /db_xref="ENSEMBL:ENSMUSE00000639600"
                     /rank="13"
                     /note="ENSMUSE00000639600"
     exon            20201..20360
                     /db_xref="ENSEMBL:ENSMUSE00000639601"
                     /rank="12"
                     /note="ENSMUSE00000639601"
     exon            19912..19996
                     /db_xref="ENSEMBL:ENSMUSE00000639602"
                     /rank="11"
                     /note="ENSMUSE00000639602"
     exon            19672..19734
                     /db_xref="ENSEMBL:ENSMUSE00000639603"
                     /rank="10"
                     /note="ENSMUSE00000639603"
     exon            19282..19413
                     /db_xref="ENSEMBL:ENSMUSE00000639604"
                     /rank="9"
                     /note="ENSMUSE00000639604"
     exon            19112..19172
                     /db_xref="ENSEMBL:ENSMUSE00000680963"
                     /rank="8"
                     /note="ENSMUSE00000680963"
     exon            18563..18727
                     /db_xref="ENSEMBL:ENSMUSE00000680964"
                     /rank="7"
                     /note="ENSMUSE00000680964"
     misc_feature    18267..22326
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaggaac agttataggc aatgtgtttt cttgaagcag tagtgctcct
     4321 ttgtcattca tcatggctgg agttgaacgt ccactggaaa atgtgctgtc tatgtccatt
     4381 ggtgtactgc ttatgctgac ctaaaatagg aataggtgct cctttaatcc gagcccttgg
     4441 gtggccgagg caggagggtc tccctgtagg taggttcttc ttcttcttct tcttcttctt
     4501 cttcttcttc ttcttcttct tcttcttctt cttcttcttc ttcttcttct tcttcttctt
     4561 cttcttcttc ttcttcctcc tcctcctctt cctcttcctc ctcctcctcc ttcttcttct
     4621 tcttcttctt cttcttcttc ttcttcttct tcttcttctt cttcttcttc ttcttctttt
     4681 cttcttcttc ttcttcttct tcttcctctt cctcttcctc ctcttcctct tcttcttcct
     4741 cctcctcttc ttcttcctcc ttcttccctc ttccctcttc cctcttccct cttccctctt
     4801 ccctcttccc tcttccctct tccctcttcc ctcttccctc ttccctcttc cctcttccct
     4861 cttccctctt ccctcttccc tcttcctctt cctcttcctc ttcctcttcc tcttcctctt
     4921 cctcttcctc ttcttcttct tcttcttctt cttcttcttc ttcttcttct tttcttcctt
     4981 ttttttgttt tcttgttttt ggtttttttg ttttttggtt tttcaagaca gggtttctct
     5041 gtgtagcctt ggctgtcctg gaactcactc tgtagaccag ccaccacgcc cggcttgtag
     5101 gctatcttct taattagtga ttgatgaaga cagaggaaaa accagcccat tgtgggtgag
     5161 gaagcccctt ggctagtagt cctaggttct atgtttaaaa caaacaaaca aacaaacaaa
     5221 caaacaaaca aacaaatcag caggctgagc aatccagtaa gcagcactcc tccgtgccct
     5281 tcacatcagc tcctgcctca aggttcccaa cccagttctt gttcctgcct tggcttcctt
     5341 tgatggactg tgtgtgaatg ttaaccaatt taaccctttc ctccccaagt tgtttttgct
     5401 caacgtgttt caaaacagca gtagttaacc ttaactaagg cagaaattgc taactgggag
     5461 agggatttac tgtgacagac ctgaccatgt gtttttggag gactacggaa gagctttgta
     5521 actgtgggct agaaaagcca ttgggtcttc aaagcttggt gagcagctct gtaggagctt
     5581 ggagggtaag aatgcagaga gcagtcagat gaaggaggcc tggcttggga agttccagag
     5641 ggaagtttgt gagccattgc atatttgaat taagagtctg taattgactg tgattaacaa
     5701 gataccaaaa cctctaatgt gtaaactatt cttttttgtg actatcgaca ctggtcaact
     5761 caagctaaga aattagtggt gattaagaag agccccgtgt tgctagagta aggtcttctg
     5821 ggtagtgttt cttcagggtt aacacatagc tgtttctaga ggagcctaca ttgtacctca
     5881 tgctggcgta gaacttggtt atttctaaga gtctctcagg tagtaatggt tttgaacgca
     5941 tgaaggggtc atggtgagca gctgaggctt ggcactatga gaggtctgga aaggtcatgg
     6001 ccaagatata gcctgagtag cagttaaagg cccaagattg aagggttggc accataaaga
     6061 cagcctaggg gaggctattg gtgaaagtgc accctagttg catcaggaga ctgacagtgc
     6121 attttggaga tgccagtacc atgggatggt cacaaaggac agaaacagcc ttggagtgga
     6181 gctgggctga gtctttgaga caagctttgt gtactgtggc aagaccttcc ctttggagga
     6241 gcccagaaga ttgtgagtta atcccagtct gcttttgctt tgtttagatt gtgtttgtgc
     6301 cctggttctt ccttcttgga gtaagaaagt gcatgatgta tttttgattt tacaggaccc
     6361 cacaattgag agacttcgaa cctttagaga ttttggaatt ttatagagac tgttatttta
     6421 gaaaagattg attttttttt tcaagacagg gtttctctgt atagccctgg ctgtcctgga
     6481 actcactttg tagaccaggc tggcctcgaa ctccgaaatc tgcttgcctc tgcctcctga
     6541 gtgctgagat taaaagattg tgccaccaca cccaaggaga ctgaattttt ttaagtgttt
     6601 gaatttgtaa agattgtgag acttttacgt ttccaatatg attcacattg tgttatttat
     6661 taatctggga ttttggggat gaacaaaaaa ggagaggcta ctgttaaata ctgttgcctt
     6721 ggtgtgtcaa attgacaagg agtcaattgt tctgggtagt tttatgtcat cttgaaacaa
     6781 gctgatgtct tctgaaagga gagaacctcc tttgagaaaa agcccgtggg ggtatcttct
     6841 taattagtga ttgatgggag agagcttagc acctcataga tggagacaat cctgggcctg
     6901 tctctgtagt ctctggttct tctcaagcca cgaggaacaa gccagtaaac agaactccat
     6961 agcctctgca tcagctgctg ccttgagctt tctgccccgt gtgcattcct gctcttttta
     7021 atttggaact gcgggtgaag taagcccttt cctcctcaag ctgcttttaa tcatggagtt
     7081 tcatcacagc aatagcaacc ctaagacaac tctctaagtt acttctggtc atggtgtttt
     7141 atcactgtgc tagaaagaaa gccaaagcaa tgccttggag gcctgcgagg ctagcgtgga
     7201 ccacactgag gtcttctctt ctccatccct acctatccca gctcccatcc tagtgaggct
     7261 caagctcctt agcacccgca gacagtacag gcagagttct gtgctgacca cagggacctt
     7321 atcccccaca ctgctcagcc tcgatccttc cccaaccgtg acctccaggc ctgccctcct
     7381 gtgtgtttga ctacagggaa gggattgctg aacctgctgg gcgagtccag tgtgagtcac
     7441 gggcaacact ggtgggcaga agggcaagca ggcagcctga gagctggtgg tggcactgtg
     7501 accatggctc tgactgtgtg tgtgaggcac ctaacagggc tgccgggtac ccatgaccga
     7561 caagtgaggc tctgctttcg aggtgagact tgtatcgttg gtctgtcatt ttagaactgg
     7621 ggagttccac attcttcatt ccctagacct ccaacctaag tcctgggcag ctctctctaa
     7681 cctgtctccc ttctccctgg cttctacttc tttcctatct tcgacttttc tctaagtgtg
     7741 agaggggaac tggacagtgg gacaggggtg ggggaggcaa atggctgtgt atatgtatag
     7801 gggctgacaa atgactagtt tcatttttga gcaggcttta cccagaaaac aaggaaaatt
     7861 cactgtggcc gagaagcaga cgtgggtgag gtcagtagag ctggttcttt tccgggtcta
     7921 tcctggggca gaagggctca ttcctgggct tggaaggtgt ggatctcagg ttggacagtt
     7981 cagacctggt ttgagatgtt actctcaaaa ctagcgaggt tagttcttga ttggcaatca
     8041 gtcccatatc agccctggat tgacaccatc tccatgtagg agggatttgt cccaggcagt
     8101 cctagaggta gcctcttgtg tggactgtcg ggaatagact ctggattagg tgtgagggtc
     8161 agtcagcctg gcacctggca tcatgaacat cttcctgttc ctagctgttt cgttggcccc
     8221 actatggatc tccactggct ggggagagtc tgtctgtgca ggtggtcaac tgtagccgcg
     8281 tgttcagccc caggtgagga gatgggagag aagccaggtg atggcttggt gtcctcccac
     8341 ctggctcagt cttttgctcc gtgcccttct tctaggcccc tggggaccct ggtgatttcg
     8401 ctgcagcagc tacagagtgc tgggcatttg gtgttgaggg aagctctggt ggacgagaga
     8461 ctccgggtgt ccccggtaag tggacagcct accttcagca acccgagatg cctccagaca
     8521 gaggttttgg attaacggag aagaaatcct gagccagaca ggagcggaaa caagcacctg
     8581 gcccagcttc agttctgttt gcagcccctg cctgagttct ccatgggcca gaagctcatt
     8641 tctgggtccc ggggtgtgct gtgtgtacta ttgctccttg ggtttggggt aggctcactt
     8701 cttttcttat gggagacatt ggtgaagctg atagggtttt cctggcattt catagaaggt
     8761 catatatcct tagaagagtt ggacttggct tcaaatcact gttacttccc tttggtgtac
     8821 ctgaaagagt tattagcatc tctaagctgt cctcctcatt tacatatggc aaatcagtct
     8881 tacacttgac tttttttttc attcgatcgt tggttaagtt aaaggatgat gttgagcaac
     8941 gaggattcag tggtaagcaa aatgagataa agatttgctt tgttgaagcc cacaaactgc
     9001 atgagggggt caaacattaa gcaagcatcc atccacacaa gacaaagtaa gtttgccacc
     9061 atgagtgtta cagaagagcc gttacaaggc tgtgataggg ggaggtctga agactttccc
     9121 tgtggaaact atgcctgtgc tcggaactga gtagaaggga gcgcgttcag acagaagaac
     9181 caaaaaacgc cggtttgctg atgttggaag ttggggtgca gagtagggca gaaacctaga
     9241 aggatctgga actgggaaag ctgtcaggga gcaggatgca agggtcaaga acagtgaaag
     9301 aacaggatgg gtggcatgca cgctgaggtt gagccctgtt gtttgtttgt ttgttatatg
     9361 agtgtttgct tttgtatatt ccaagcttag gtttgattgt tttcagtgct gctgagcctg
     9421 aactcaagcc tttctgaatg ctggccaagc actctcccaa ctgagctgtg cccccagcct
     9481 caagcctatc tccattttat ctgtagctga gtgtggtagc acatatcttt aatccgggca
     9541 gaggcagagg caggaggcag gaggcaggag aggcaggaga ggcaggagag gcaggagagg
     9601 caggagaggc aggagaggca ggagaggcag gagaggcagg agagggagga gaggcaggag
     9661 aggcaggaga ggcaggagag gcaggagagg caggagaggc aggagaggca ggagaggcag
     9721 gagaggcagg agaggcagga gaggcaggag aggcaggaga ggcaggaggc aggaggcaga
     9781 ggcagaggca gagggtcagg cagagggtca gtcagagagg cagagaggca gagagagagg
     9841 cagaggcggg tggatctcta taagttcaag gctagctaga gctacacaat aaaacccttt
     9901 ctcaaaaaaa tttttaaatg tttattattt tattttttgt taagagttat gtattcatgt
     9961 attttatgta tatgtgtcca gactccatgt acatctgcat gccaggagaa ggagtggagc
    10021 ccattacaga tggttgtgag tcaccatgtg ggagctggga attgaacccc ggtcctctgg
    10081 aaaagcagcc agtgctctta accactgagc catctctcca gtcctttaaa catttatttc
    10141 ttatgtgtac atgagccttt gtgtagaagt tagagaacac cttctgggag ttggtttttg
    10201 gcctccatta aggcgcataa cgataccacg atatcaacaa gtttgtacaa aaaagcaggc
    10261 tggcgccgga accgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
    10321 cgaacccttt cccacaccac cctccacact tgccccaaac actgccaact atgtaggagg
    10381 aaggggttgg gactaacaga agaacccgtt gtggggaagc tgttgggagg gtcactttat
    10441 gttcttgccc aaggtcagtt gggtggcctg cttctgatga ggtggtccca aggtctgggg
    10501 tagaaggtga gagggacagg ccaccaaggt cagccccccc cccctatccc ataggagcca
    10561 ggtccctctc ctggacagga agactgaagg ggagatgcca gagactcagt gaagcctggg
    10621 gtaccctatt ggagtccttc aaggaaacaa acttggcctc accaggcctc agccttggct
    10681 cctcctggga actctactgc ccttgggatc cccttgtagt tgtgggttac ataggaaggg
    10741 ggacgggatt ccccttgact ggctagccta ctcttttctt cagtcttctc catctcctct
    10801 cacctgtctc tcgacccttt ccctaggata gacttggaaa aagataaggg gagaaaacaa
    10861 atgcaaacga ggccagaaag attttggctg ggcattcctt ccgctagctt ttattgggat
    10921 cccctagttt gtgataggcc ttttagctac atctgccaat ccatctcatt ttcacacaca
    10981 cacacaccac tttccttctg gtcagtgggc acatgtccag cctcaagttt atatcaccac
    11041 ccccaatgcc caacacttgt atggccttgg gcgggtcatc ccccccccca cccccagtat
    11101 ctgcaacctc aagctagctt gggtgcgttg gttgtggata agtagctaga ctccagcaac
    11161 cagtaacctc tgccctttct cctccatgac aaccaggtcc caggtcccga aaaccaaaga
    11221 agaagaaccc taacaaagag gacaagcggc ctcgcacagc cttcactgct gagcagctcc
    11281 agaggctcaa ggctgagttt cagaccaaca ggtacctgac agagcagcgg cgccagagtc
    11341 tggcacagga gctcggtacc cggaagatct ggactctaga gaattccgcc cctctccctc
    11401 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
    11461 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
    11521 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
    11581 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
    11641 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
    11701 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
    11761 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
    11821 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
    11881 atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc
    11941 ctttgaaaaa cacgatgata agcttgccac aaccatggaa gatcccgtcg ttttacaacg
    12001 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
    12061 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
    12121 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg
    12181 gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca
    12241 cggttacgat gcgcccatct acaccaacgt gacctatccc attacggtca atccgccgtt
    12301 tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg
    12361 gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg
    12421 gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct
    12481 gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gctggagtga
    12541 cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc
    12601 gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga
    12661 tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta
    12721 cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc
    12781 tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct
    12841 gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt
    12901 tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg
    12961 cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg
    13021 cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt
    13081 gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc
    13141 gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc
    13201 caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct
    13261 accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt
    13321 gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg
    13381 ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac
    13441 cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc
    13501 ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct
    13561 gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg
    13621 gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca
    13681 gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg
    13741 cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc
    13801 gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg
    13861 gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca
    13921 ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc
    13981 tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca
    14041 actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca
    14101 catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc
    14161 gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa
    14221 taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa
    14281 aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat
    14341 tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc
    14401 gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc
    14461 ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg
    14521 gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc
    14581 gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga
    14641 gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc
    14701 ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag
    14761 cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg
    14821 cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg
    14881 ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat
    14941 tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccagctga gcgccggtcg
    15001 ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggg gggatctaag
    15061 ctctagataa gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt
    15121 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    15181 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    15241 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    15301 ttatcatgtc tggatccgga ataacttcgt atagcataca ttatacgaag ttatgtttaa
    15361 acggcgcgcc ccggaattcg ccttctgcag gagcgtacag aacccagggc cctggcaccc
    15421 gtgcagaccc tggcccaccc cacctgggcg ctcagtgccc aagagatgtc cacacctagg
    15481 atgtcccgcg gtgggtgggg ggcccgagag acgggcaggc cgggggcagg cctggccatg
    15541 cggggccgaa ccgggcactg cccagcgtgg gcgcgggggc cacggcgcgc gcccccagcc
    15601 cccgggccca gcaccccaag gcggccaacg ccaaaactct ccctcctcct cttcctcaat
    15661 ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag agggggtaaa aaaatgctgc
    15721 actgtgcggc gaagccggtg agtgagcggc gcggggccaa tcagcgtgcg ccgttccgaa
    15781 agttgccttt tatggctcga gcggccgcgg cggcgcccta taaaacccag cggcgcgacg
    15841 cgccaccacc gccgagaccg cgtccgcccc gcgagcacag agcctcgcct ttgccgatcc
    15901 tctagagtcg agatccgccg ccaccatgat tgaacaagat ggattgcacg caggttctcc
    15961 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
    16021 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
    16081 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
    16141 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
    16201 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
    16261 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
    16321 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
    16381 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
    16441 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
    16501 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
    16561 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
    16621 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
    16681 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
    16741 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
    16801 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
    16861 ggggatctca tgctggagtt cttcgcccac cccccggatc taagctctag ataagtaatg
    16921 atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac
    16981 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
    17041 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    17101 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    17161 cgggggtacc gcgtcgagaa gttcctattc cgaagttcct attctctaga aagtatagga
    17221 acttcgtcga gataacttcg tatagcatac attatacgaa gttatgtcga gatatctaga
    17281 cccagctttc ttgtacaaag tggttgatat ctctatagtc gcagtaggcg gtttaagctc
    17341 ctaagcctag tgccgttagt tctcaagaag gctgtgagca tctacaacgt gatattcttt
    17401 aataattcta tgagaattta ataggatgta tttgatcata ttcatccctc actcttccca
    17461 tgatccctct ccatcctcac ttccctaccc acccgactgt gagttttctt tatttttcac
    17521 cccttgataa aactcagttt gtgttaccca gctgctcttg ggagtaggac ctgccttggc
    17581 gtgtagccaa cctaccaggg gtcatcatta aagaaagctg ccgcagcttc ttctagcagc
    17641 agtgcagaag gttgtgagtg ctaccattat tgtaatcgga gaggaacacg ctactgcaga
    17701 catagacgga gaggcccctt ttttactgat ggtgtgcaag atgttggtac ttcttagccc
    17761 catgccagtt tgagggcacc agcgggtgcc gtatttggtt tcagatccaa gtggagcttg
    17821 acctaaagta tcaaccccct gagggagctg ctgggacctg ggcagaggag gactttggga
    17881 cacccattcg agacaggtaa tcgtctagta acctatgcaa ggcctggtgt ccccagatga
    17941 aactcgccct gaagagagag catatgcctt caccttgggt ctgcggggtg ctgtgatctc
    18001 agcactctac agtcgggtct catcttctct attttccttc ccagcttaga gttgatcatc
    18061 cccaatgtgg gcttccagga catggagtga gtatgtgggg tgatagggaa taaacagggt
    18121 cactttgggg gtgaagagcc tgtgccatga tttagtacta gagtctgaga ttccagcgat
    18181 tgaatggaga tggcgcaacg caattaatga taacttcgta tagcatacat tatacgaagt
    18241 tatggtctga gctcgccatc agttcaataa ggtcagaagt caacccttac accaagacta
    18301 ggttatggcc gggcggtggt ggtgcacgcc tttaatccca gcacttggga ggaggcaggt
    18361 ggacagcctg gtctacagag tgagttccag gacagtcagg gctacacaga gaaaccctgt
    18421 ctcaaaaaaa ccaaaccaaa acaaaagagt tagtcccagg gtaagcgggg tcaatctgaa
    18481 ggttaaaggt cagttctaag ataagcaaag gcaagggatg cccagcacca ggtgacaggt
    18541 cctatgaagc ctttcctctc aggcctgggg aagcccagct ggagcgaagg gcagtggctc
    18601 tgggccgcag gctagctcga agcctaggaa cacaggacga tgaagagaac gaattggagc
    18661 tggagccgga gctggaggaa gaacctgacg tggagatttc tggggtggtg ttcagccccc
    18721 tcaaaaggta gtgtacgcac atgctcctgg tctcacaccc tgtcgctgag tcagttttcc
    18781 tcacacacat aagccacacc ccatccatcc acaccagggt acccggaagg gtggatacac
    18841 tggcatcaaa gatcattcac ttacagcaca gacagacact accatgttca aagtcactta
    18901 tagtggctca caacgatttg taaaggaatt tgataagggc aggttgtgtc tgttgttctt
    18961 gttcagacct ccaagagtga atcgaattct ggatgtaggc ctgagatgga tgtagggcaa
    19021 ggcactctga cgcctcatcc cagcgacgag catctgtgtg tgtgggtggg agaggaggga
    19081 agagcagagc tgaccctaga cttctttaca gccgcgccct gggcttgcct cggggagacc
    19141 ccttcaaggt ttgcaaagcc caggacttcc aggtatgact ctcaggccat attctaaccc
    19201 tcgtggcttc tcacacgggt ctctcccctt acccaggcag ctctgacttt ctttcctctc
    19261 tttgtttcat tccttaccta ggtcggggtc acagtgcttg aggcccagaa actggtggga
    19321 gtcaatatta acccctatgt ggctgttcgc gtcggggatc agcgcaaggt gactgcaaca
    19381 ttgcgtggaa ccaattgccc cttctacaac gaggtgaggc gccaccaagg aagagggcgg
    19441 gtctgagaga atgggaggga tccagagaga cctgtgacta gagagggcgg ggccaggatg
    19501 caaagtcgct ggttctgcgt tttgagccag gttaggggag tcatcccggg gaaagagtac
    19561 gtaggtgcct tttaggaaag ggaccgcatt ctctatgcaa gaattcgggg cgagctgaat
    19621 gagcctgtcg tgcctaacct cctctcgctg ctctaacctt tctcatccca gtacttcttg
    19681 ttcgaatttc atgagactcg gcttcatctc caagacttgc tgctggagat aactgtgagt
    19741 gggaaagcag attcaagtca ggaaagggag gtatcgagct gggcatttgg gtgactgaga
    19801 caggaaaatc gccatgagtt caaggcccag ctagggctat agactgagag tcaaaaaggt
    19861 ggacagagag aaagatgcca aagcagcagg acttaaccct tctctcccca ggctttccac
    19921 tcgcagactc tcccgtttat ggccaccagg atagggactt tcaggatgga cttggggatg
    19981 gccttggacc agccaggtat taaacccctc ctctctattt atatgaacaa attaactgag
    20041 ttctggccag tggactaact tcgaagacag ccgtctcttg tgaatgctca ccttttttgt
    20101 atggtggttc tccctgctcc aaccccagtc attgtccaac atcctggatc tgtgtccctt
    20161 agatgtctcc tcactctctg tcctcttcct ctgtccttag atgggcactt ccaccaaaag
    20221 tgggctcctt tgcatgaccc ccgtgacacc cgcgccggaa ccaagggctt cgtcaagatc
    20281 accctgtctg tgagggcacg tggggacctt ccccttccac cgccaccccc atgcccaggg
    20341 accagttcgg acatagagaa gtgagctggg cggtggaggg aggagaaatt ggataaggcg
    20401 tggcctgggg gcgaggcaga gaagcagagg gggctcaggc tccaacttta ttccccacgt
    20461 tggcttctcc taggaatcta cttctgccac atggggtgca ggctgagagg ccatgggcac
    20521 ggctgcgagt ccgcgtgtac cgtgcagagg ggctccccac agtgcgcacg ggcctgctgg
    20581 gcagcctggc ccgagccctc catgaccaaa atgtcctttt ggacccgtat gtgcgggtga
    20641 gcttcctggg acagcaggta agtcactcct atgttccgtg ctgcagctca agtcttttaa
    20701 aaagtattaa ttaatgataa tcattattta ttcatttaga gacaaggttt tgctatgtag
    20761 ccctgatact cagagatccg tttggtagga ttacagggat ctgccacaaa ggctggcttt
    20821 tggttgttgt ttatttgttt ttcaagatag ggtttctttg tgtataacct cagctgtcct
    20881 ggaactcact ttggatacca ggctggcctg gaactcagag atccgcctgc tactgcctcc
    20941 caagacaatt tttattctta attgtgtatg tatgaaaatc tctgtggaat gcttgagggc
    21001 agaagctcat ggaggcccga agagggtgtc agccatccct tctagctgga gtcacaagtg
    21061 tctgtgggct gctggatgag tggaggaaaa gcaaactcac ttgggagagt agaactcacg
    21121 cttgacctta actactgagc catctctttg gctattttgt gtatgtatgt ggtgctttga
    21181 gtgccacagc acatgtgtga aagtcagagg acatcctata ggagggacaa cagggatagg
    21241 acctagggat caaactcagg ccatcaggtt gatatcaatc gtcattgcct gctgagccat
    21301 cttttaggac ccgcactgac ccattctgcc tgacttggcc ttcagggtga gacttcggtt
    21361 cggggcgagg agacagcgcc caagtggaac gagcagttga gcttcgtgga actcttcccg
    21421 ccgctaacac gcagccttcg cctgcagctg cgtgacaatg cacctcttgt ggatgtggcc
    21481 ctcgccacac acgtgctaga cctgaggcag atctcgaact cgggtcgcgc aggtgagcac
    21541 gtctggtccg cgagggtcct tgtctgccat ggctcacatc agctcctcag agcaaagctc
    21601 aacctttacc acaacaccag tacttccacc ccaacctcat ctcctcccta aagcccagag
    21661 aggccctctg accgatactg ttagcaccac tcccttcaga aagaaccaaa ccctgccttc
    21721 tcttccccgc cccagcacct ggaggataca agtgtgtaca gttcagaaca gtacccattt
    21781 attctgtgct cccctccaat cctgttcttg tcccctcaca gcggggttta accccacttt
    21841 cggtccagcc tgggtgcccc tctatggctc gctccccagc ggcaggctcc gggatgatct
    21901 tcaaagtctc aacgaaggtc ttggcgaagg catttggttc cggggtcgac ttctggtagc
    21961 tgtgtccatg gaggtgtacg aaggcagagt tgaacctaaa ccctcccaga ccacacaaag
    22021 gtctggattg tcccggctca ctgggaagaa gaaaaagaag aaggagaaga ccagacaggg
    22081 tcagaccccg gctggcattt tacagcctgc cagtgccagc accagcgagg atgcgcctga
    22141 gattccgagt gcaatggagg tggaggtgga ggacctgctg cctctgccag aggtaggggc
    22201 tggagtcacc ctcagcgtca gacatcatga tgctctttcc agtttgggga cttggatgaa
    22261 gagtcctgtc atgtgcacac gtgtgctggt gtatctaagc atggtatata ttaaggaggt
    22321 cagttc
//