LOCUS       KO-first_condition_ready_83024_MGI:1336882        21792 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Vamp8 targeting project(s): 83024
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 43248
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7130..7359
                     /db_xref="ENSEMBL:ENSMUSE00000698105"
                     /rank="1"
                     /note="ENSMUSE00000698105"
     misc_feature    4275..8993
                     /note="5 arm"
     misc_feature    9021..9045
                     /note="B1 Gateway"
     misc_feature    9058..9105
                     /note="Frt"
     intron          9106..9980
                     /note="mouse En2 intron"
     misc_feature    9978..9980
                     /note="Splice Acceptor"
     exon            9981..10153
                     /note="mouse En2 exon"
     misc_feature    10165..10758
                     /note="ECMV IRES"
     gene            10759..13819
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13858..14101
                     /note="SV40 polyadenylation site"
     misc_feature    14105..14138
                     /note="loxP"
     promoter        14165..14684
                     /note="human beta actin promoter"
     gene            14710..15504
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15704..15945
                     /note="SV40 polyadenylation site"
     misc_feature    15962..16009
                     /note="Frt"
     misc_feature    16016..16049
                     /note="loxP"
     misc_feature    9995..10072
                     /note="FCHK std_gpr"
     misc_feature    16065..16085
                     /note="B2 Gateway"
     misc_feature    8994..16115
                     /note="Synthetic Cassette"
     exon            16514..16672
                     /db_xref="ENSEMBL:ENSMUSE00000388617"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000388617"
     misc_feature    16116..16875
                     /note="Critical Region"
     misc_feature    16899..16932
                     /note="Downstream LoxP"
     misc_feature    16876..16955
                     /note="synthetic loxP region"
     exon            19307..19737
                     /db_xref="ENSEMBL:ENSMUSE00000804254"
                     /rank="3"
                     /note="ENSMUSE00000804254"
     misc_feature    16956..21792
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacccagc actggaatcc aaagacaaaa gagtaaagtt gttattagtt
     4321 gtgtagttta cattttctag cagagaggct ggaggtgcag tgtgaatgtg gctcgggagg
     4381 tcgagggctc ctctggcact gcagggagca ctggactctc tttccagcta ggaaagaaaa
     4441 tgtggctgac tggctgattg attaactgac tgactgattg attgatgaat gattgactgg
     4501 agacacagtc tggctttgta gcccaaggta gccttgaatg tgtgattgcc ccggctcaga
     4561 ttcctgagtt ctggagcagc actgcacaag gctgtaaaac gttcatgaca tttatgaaag
     4621 tagcgttctt cttttcgaac taagttgctg taatctgttg tgcattttcc agcctccgtt
     4681 ctcactagca gcgctgggag agctcactgg ccgtatggag tcagcgttgt actgggcagc
     4741 aaaagaacag agcaagcatt tgggggtggg aggtcttggg acagaaccca gggctttggc
     4801 gattctaggc aaactctgtt gctactgaga gagccttcga cactgagaca gggtcttgag
     4861 acgtagtcct gcctgtcctg tatctcattt tgtagactag ctggcctccc agagatcccc
     4921 ctgcctctgc ctcccaagat gttaaaggca ttcgtcacca cactggccca ggcaggttct
     4981 gtcttataaa cctccttgtg atttaagatg aatcttttaa aatatagagt tttttttttt
     5041 tttttatggt tttttgtttc tgttttttgt ttttttcgag acagggtttc tctgtgtagc
     5101 cctggctgtc ctggaactca ctttgtagac caggctggcc tcgaactcag aaatccgcct
     5161 gcctccgcct cccaagtgct gggtttaaag gcgtgcacca ccactgccca gctactgtta
     5221 ccttttaact atgtatgtct gtgtttgtgt gtatgaatag ggtctgtggg gccagaagag
     5281 ggcgtcagat ccctggagct agagttacaa gtaatgagtt gcctgaccca ggtgctggga
     5341 atcaaatttg ggttctctgg aagaacaata catgctttta accaaagggc catctctcca
     5401 ggacccagtt ctttctttgt gaccactgag cccttgatca gcaggatggg gacattctca
     5461 aggcctcttg accttttgcc taagatcaag tgtagtacag acattctctg ttgtgatctc
     5521 attcctcttt attctagatt tttcttttgc tttataggaa aggaaaggag ttgtgccgct
     5581 ttaaggtgtt actaggggct agagagagag ctctacagct aagagcacgt gctactcttg
     5641 catagaaggg tccagctccc agcacccaca tcaggtgtct cacaactgtc tgcaactcac
     5701 cagagaacct aacccctctg ccctcaggca cacacacaca cacacacaca cacagacaca
     5761 cacacagaca cacacacaca cacttaaaga taataagata aaaactttta gccgggagtg
     5821 gtgatgcaca cctttaatcc cagcacttgg gaggcagagg caagtggatt tctgagttcg
     5881 aggccagcct ggtctacaaa gtgagttcca ggacagccag ggatatacag agaaaccctg
     5941 tctcggaaaa aaaaaacaaa caaataaaca aaaaaaaaaa aaacccaaaa aaaaaaccca
     6001 aaaaaaccaa caacaacaaa aaaaattttt aaagattttt ttaaagtgtt actagttgct
     6061 ttaatttgct tctgtaaaac taagcgtgca gtgtttgtag atatagctcc tccatggctg
     6121 cctgagcagg cacgagggaa aacacataag tgtcccaaaa tgattccggg attctaaatt
     6181 tattatagcc aacaaaatga aacaaaacaa aacccctctt atttgttgga ggagctaaga
     6241 tttcctcctt gacggtgtta tttactctgg ggtgggaaag aatgtctgtt ctctttctgc
     6301 cctgttattg ttctgtcttg acaaactcca tccggagaag agcagatttt ctctggcgga
     6361 gagccacatg tggtggaaaa accctttact cttaggcccc catcctaatc tgtctgacaa
     6421 ccctctgaca actgaccagg gtcaccaacc cccacggtga cctttggaag gaagtgtagg
     6481 actgctcaac tccagaagga aaggaggggc tggtgagtga gccatttccc cgactgtcct
     6541 gtgtcctgtg acaaggataa ggtgccctgc ccctgctgtt tgcactcctc ccactgcctg
     6601 aggtcagcct tagcacccga agaacattag agggggacaa agatcccacc cccagggcca
     6661 acttggaatt ttcctggaga ttttaaagac tttaattatg tgtatgtgtg tgtatgtatg
     6721 cacacgtatg ttcacatacc cacagaggcc agaagagggc atctggaccg cccctgcccc
     6781 cccccccccc ccgagatgca gatacaggtg tctgtgagca gccaggggtg ggtgtaggga
     6841 cctgaacctg ggttactgca aaagcagcgg gtgatcccaa ctgctgaccc atctccctag
     6901 cccccgtttt ctgggggatc ttagagctct cttctcatac aggatggccc ttggggacaa
     6961 aggttgccca acaaggtatc ttcgcctgta cattcgtttc tatctaaata atgctaatgt
     7021 aagtccctga aaaactagaa ttacttcttt gaagcataaa agaagtttct gaaaacttag
     7081 gaggactgtt gatccttctt ttctccttcc cccctccccc aaccctgttg cttaagcttt
     7141 tgtttgggag gagactagga ggacggcagc agagggcagc cgcctacctg gagaaacctg
     7201 cagttacgtg tgtggcagga gccggagtgg ctggagtcgc gggatggtca ggaggcgggc
     7261 tgaaagttgg agtaagcagg aagtgaagct gtggctgcct tgggtggaaa cagactaggc
     7321 gaagttctgc tttgagaggc ccaagtgctc cgagacatgg tgagtgacgt ggggaccaag
     7381 aaaagagcga gttagagcag ctgccagagg gggatcgggc ctgggggttg cctgtgtcga
     7441 gaaaggtaag ttggcggcag tcagagggct ggcggggttg ggccagcgct gcctctgtct
     7501 ccttactttt gacaactcct ccacaaatag ctccgcactc cgggccaggg gttcctgaca
     7561 cttctccacc tccttcctcc cgtgacctcg gtgctggatc cgatcaggat ttcggtgggg
     7621 gttgggggac ccaaattcca agccgagctg ctgcgccgcg ccctccccct cctccgcctc
     7681 ctccagctgg gcctgagccc ggtgttgtga cattaccggg ccacaagcgt tcctgctctt
     7741 tggggctcag attccagccc cgcggactgg agttctcccc cttaccctcg cactgtatct
     7801 atgcagacac ccagaggatc tatctgcctg gccaccaaaa gcaccaccct tttctagaaa
     7861 cattagcaac agtgccaagg gtttgtaaag cagccagatg gcggttcttc ccgataatct
     7921 agcttcagcc cacacccgtg gccggtacct gaggccccct gggtccctaa gggaggagtc
     7981 aggcctcttc cctccttgtt caaaagcctt atctagaggc ctcctggata gagcgaccgc
     8041 agcaggaagg acttttatta atatctgcat aagcaggcgg tggtgtgcgg cctaccaggc
     8101 gttaaattgt ggggaaagca gaagtatatg gtccaacggc acatgatgcc ttctgggggc
     8161 cacatccact gaaccggggc tacactgccc agtaggctgg aaccagaact caccaacagc
     8221 tgtctggaga agggcattta tttgggttgg gccgggcaag agtgtgctgc tggtcaactc
     8281 tcacagtttt agagagaacc cactaggatt gagccgaggg aggctacagg aaccagttaa
     8341 tagttcttgg ctgtgctctg ctgaggaaac gtcagctggg ttttctcctt gaccctcttt
     8401 gtggagtcaa gacaggggca gtcgtgtgct gtttttttgt ttgtttgttt tttgtttttt
     8461 gttttttttt gtttttcctt gtcacgctga aaatcctcca aagaaggtgg atgagaatcc
     8521 agggtcaaac ctcatcttct gggacttaaa aaatgcactt acatggctgg cagccgagat
     8581 ggctgggcag gtacaagtgt ttgcagagca agcctgcctg cctgaattca gtatcctgct
     8641 ctctcctgcc catccaactg gaggagacaa tccactcatg aaagttgtcc tctcacctcc
     8701 acatgcgtgc cgtggcatgt gcatacacgt atattcccca gcacacacac acacacacaa
     8761 tgcacttatg acagactaag gatgtggttc agctgataga gtgtttgcct ggcaagcgtc
     8821 aagctctggg tttcatcccc agcacctcat aaaacaagga taatggtaca cacctggaat
     8881 cccagcccag ggtaagtaga gacagggggt cagatgttta aggccatcct acttaggaag
     8941 cctatggcct gcctaggtta tatgagaacc tgcctcagat aaataaatct gggaaggcgc
     9001 ataacgatac cacgatatca acaagtttgt acaaaaaagc aggctggcgc cggaaccgaa
     9061 gttcctattc cgaagttcct attctctaga aagtatagga acttcgaacc ctttcccaca
     9121 ccaccctcca cacttgcccc aaacactgcc aactatgtag gaggaagggg ttgggactaa
     9181 cagaagaacc cgttgtgggg aagctgttgg gagggtcact ttatgttctt gcccaaggtc
     9241 agttgggtgg cctgcttctg atgaggtggt cccaaggtct ggggtagaag gtgagaggga
     9301 caggccacca aggtcagccc ccccccccta tcccatagga gccaggtccc tctcctggac
     9361 aggaagactg aaggggagat gccagagact cagtgaagcc tggggtaccc tattggagtc
     9421 cttcaaggaa acaaacttgg cctcaccagg cctcagcctt ggctcctcct gggaactcta
     9481 ctgcccttgg gatccccttg tagttgtggg ttacatagga agggggacgg gattcccctt
     9541 gactggctag cctactcttt tcttcagtct tctccatctc ctctcacctg tctctcgacc
     9601 ctttccctag gatagacttg gaaaaagata aggggagaaa acaaatgcaa acgaggccag
     9661 aaagattttg gctgggcatt ccttccgcta gcttttattg ggatccccta gtttgtgata
     9721 ggccttttag ctacatctgc caatccatct cattttcaca cacacacaca ccactttcct
     9781 tctggtcagt gggcacatgt ccagcctcaa gtttatatca ccacccccaa tgcccaacac
     9841 ttgtatggcc ttgggcgggt catccccccc cccaccccca gtatctgcaa cctcaagcta
     9901 gcttgggtgc gttggttgtg gataagtagc tagactccag caaccagtaa cctctgccct
     9961 ttctcctcca tgacaaccag gtcccaggtc ccgaaaacca aagaagaaga accctaacaa
    10021 agaggacaag cggcctcgca cagccttcac tgctgagcag ctccagaggc tcaaggctga
    10081 gtttcagacc aacaggtacc tgacagagca gcggcgccag agtctggcac aggagctcgg
    10141 tacccggaag atctggactc tagagaattc cgcccctctc cctccccccc ccctaacgtt
    10201 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
    10261 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
    10321 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
    10381 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
    10441 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
    10501 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
    10561 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
    10621 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
    10681 ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
    10741 gataagcttg ccacaaccat ggaagatccc gtcgttttac aacgtcgtga ctgggaaaac
    10801 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
    10861 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
    10921 cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt
    10981 cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc
    11041 atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat
    11101 ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag
    11161 acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg
    11221 gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc
    11281 gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa
    11341 gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg
    11401 actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct
    11461 gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt
    11521 tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt
    11581 atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg
    11641 aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc
    11701 gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa
    11761 aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag
    11821 catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg
    11881 atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg
    11941 tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac
    12001 ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa
    12061 cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg
    12121 gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc
    12181 gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt
    12241 atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg
    12301 tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac
    12361 gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag
    12421 tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat
    12481 gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat
    12541 cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg
    12601 gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg
    12661 accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg
    12721 gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag
    12781 ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta
    12841 cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag
    12901 cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg
    12961 catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt
    13021 aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg
    13081 ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg
    13141 acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc
    13201 gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc
    13261 gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt
    13321 gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat
    13381 ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc
    13441 ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg
    13501 gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc
    13561 tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac
    13621 atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg
    13681 gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc
    13741 tggagcccgt cagtatcggc ggaattccag ctgagcgccg gtcgctacca ttaccagttg
    13801 gtctggtgtc aaaaataata ataaccgggc aggggggatc taagctctag ataagtaatg
    13861 atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac
    13921 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
    13981 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    14041 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    14101 cggaataact tcgtatagca tacattatac gaagttatgt ttaaacggcg cgccccggaa
    14161 ttcgccttct gcaggagcgt acagaaccca gggccctggc acccgtgcag accctggccc
    14221 accccacctg ggcgctcagt gcccaagaga tgtccacacc taggatgtcc cgcggtgggt
    14281 ggggggcccg agagacgggc aggccggggg caggcctggc catgcggggc cgaaccgggc
    14341 actgcccagc gtgggcgcgg gggccacggc gcgcgccccc agcccccggg cccagcaccc
    14401 caaggcggcc aacgccaaaa ctctccctcc tcctcttcct caatctcgct ctcgctcttt
    14461 ttttttttcg caaaaggagg ggagaggggg taaaaaaatg ctgcactgtg cggcgaagcc
    14521 ggtgagtgag cggcgcgggg ccaatcagcg tgcgccgttc cgaaagttgc cttttatggc
    14581 tcgagcggcc gcggcggcgc cctataaaac ccagcggcgc gacgcgccac caccgccgag
    14641 accgcgtccg ccccgcgagc acagagcctc gcctttgccg atcctctaga gtcgagatcc
    14701 gccgccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
    14761 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
    14821 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
    14881 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
    14941 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
    15001 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
    15061 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
    15121 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
    15181 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
    15241 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
    15301 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
    15361 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
    15421 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
    15481 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
    15541 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
    15601 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
    15661 agttcttcgc ccaccccccg gatctaagct ctagataagt aatgatcata atcagccata
    15721 tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
    15781 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
    15841 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    15901 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccggggg taccgcgtcg
    15961 agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcg tcgagataac
    16021 ttcgtatagc atacattata cgaagttatg tcgagatatc tagacccagc tttcttgtac
    16081 aaagtggttg atatctctat agtcgcagta ggcggtacgc aagcatgaag atttgcattg
    16141 gatccctaga cccatttaga aggaaaaaaa ttccaggtgt ggtggtgcaa gcaagcactt
    16201 aggtggctcc tagtgctagg gtggtggaga aggctgagcc ctggggcttt ttgtccactc
    16261 taatctcaga gcctcaggcc aacaggagac ccagcctgtg ggggaaaaaa caagggagga
    16321 gtgacacctg accacccgag gctgatctgg gatataaacg tgcttgcatc gaagtgaaca
    16381 tttacgtaga acattctcct ggtaaacagt tgaagcatca gaaacggcta aaatctactt
    16441 tagttcttcc aacctcctct ggcctcttgg atcctctgct gcccggccta attaacccct
    16501 tattgacatc caggaggagg ccagtgggag tgccggaaat gaccgagtta ggaacctgca
    16561 gagtgaggtg gagggagtca agaatattat gacccagaat gtggagcgga tcttggccag
    16621 aggggagaac ctggaccacc tccgaaacaa gacagaggac ttggaagcca cggtgagatt
    16681 gggagaaaac tgggagttgg tgggggcaga tctcggggag ggggagaagt ttgggtgggg
    16741 aggcaaagtg aacagaaggg actgcagact gtcatgtcta ctgccctttt gcctatgcag
    16801 gagatggctg aggttccacc tctgcttcct tgctactctg ctggctggta gagagcccag
    16861 tgagtggttg gatttgagat ggcgcaacgc aattaatgat aacttcgtat agcatacatt
    16921 atacgaagtt atggtctgag ctcgccatca gttcatgctc cattcctaag cttcattggg
    16981 gtatgaaact gatttttgtc tcaaagcttg cccttggcaa ccatttggag ttgatgtccc
    17041 aaaggcaaag ttatgagttc cgggcaaccc ttggcctttc agcattctcc ctccaggtgt
    17101 aattaggctg gaagttggtg gggggcgtct tgctgagtcc gctttaaagc acagctggga
    17161 agaccaatga aatcctttaa ccccaggatc cctgtgtgca aaggccaaaa taatatctcc
    17221 cacacatctt gcttagacct cctctttgct gccatggact gaggccaagc tgctcagggc
    17281 agaagaaagt ctaacacaaa ccaaggctgg ggcctcagtt ttcacttgct gtggagtaca
    17341 ctggcttcct gggtccattg ggttatcttg gaatctagtg cctcctgcag cataagctgg
    17401 gcacatggag acttagtgtc tgtattaact tggagaagca ggaggctttt ttctttcttc
    17461 ctttcttcct ttcttccttt cttcctttct tcctttcttc ctttcctttc ctttcttcct
    17521 ttcttccttt cttcctttct tcctttcttc ctttcttcct ttcttccttt cttcctttct
    17581 tcctttcttc ctttcttcct ttcttccttt cttcctttct tcctttcttc ctttcttcct
    17641 ttcttccttt cttcctttct tcctttcttt tctttctttt ctttctttct ttctttcttt
    17701 ctttctttct ttctttcttt ctttctttct ttctcaagac agggtttctc tgtgtagccc
    17761 tggctgtcct ggaactcact ttgtagacca ggctggcctc gaactcagaa atccacctgc
    17821 ctctgcctcc caaatgctgg gattaaaggc gtgcgcaacc acgcccagct ctttctttta
    17881 agtttctgca gtgctttggt tcttccttca gcttctacag aactcagaag acaaagcaaa
    17941 gtatcattga gccaggacat gtgcccatct ggagcacatt cctgtacccc ttagcttgtg
    18001 ggtctgtgct ccaaaggcca gatttctcta ccagtcaaac aaactccaat tgtgagcata
    18061 tatctgtaaa agacccctta gtatgcaggt tagtgcaggg gcaagaaaaa gaagggagac
    18121 tggacataag actcaggtgg tagagtactt cactagactt cacgaagccc tgggttcagt
    18181 ccctggcact atataaatat ggtattgtgg cacacgcctg taatctgaac tctttgaagg
    18241 tagaggcaag tggatcagaa gttcaaagcc aggccattga gatggctcaa aaggtaaaga
    18301 cacttggtac caagcctaag gatttgagtt ccatttgtgg gattcatatg gttggtagtc
    18361 ttgattccta taaaatgacc tctgacctcc actgtggctt gtgcacacca cacacataaa
    18421 caaacaaaca aataaatatt gtttttaaaa gttcaaggtc gccgggcatg gtggattaat
    18481 cccagcactc gggaggcaga ggcaggtgga tttctgagtt cgaggccagc ctggtctaca
    18541 gagtgagttc caggacagcc aggactacac agagaaaccc tgtctcgaaa aaccaaacaa
    18601 caaacaaaca aacaaacaaa caaaagttca aggtcatcct ttgctacaag gagtggaggc
    18661 caggctttgc tgtatgagat cccgactaga aagatagaaa cagaaaagga agggaccctg
    18721 ctatgctcag agggaagttt tatgagttca agaattctaa actagaactt ggcttacaga
    18781 ttgttgtaga aagacatttg tgaagcagcc agagatagac agatctctga agctcctggc
    18841 catccaggca tacttagtga ggccctgtct caaaagtaaa acaaaacaaa acaaaacaaa
    18901 caaacaaaaa aaaaaaaaac cagagagaga tcagtgggta aagacttttg tcagcgagcc
    18961 tggctgcgtg ggttcaatcc ccaggacata cagaggagaa gagagaacca tttcctgcaa
    19021 gtcgacccct ctccaccacg tgggcagtgt ggcacataag gcagaggcac aaatacatgt
    19081 tataaagtat ttttaagaac ctttccattc ctattgtaag aggaaagaat tgttcactta
    19141 tttgtgtttt tatttcgaag tggctatcct ccagctccag cgtctttttt tccgtttcct
    19201 acttcgattt ataatgtaat gtaaacatct atactatggc atgtggtgtc cgtctgtact
    19261 ctcagtctga gggcaggagc tcacgacttt atcttatccc caacagtctg aacacttcaa
    19321 gacaacgtcc cagaaggtgg cccggaagtt ctggtggaag aatgtgaaga tgattgtcat
    19381 catctgtgtg attgtcctta tcatcgtcat cctcattata ctttttgcca ctggtaccat
    19441 ccccacttaa gagtcccttc catcccaccc tactcctcag gggtgacctc cctaatatgt
    19501 gccaagagga gccctctcct gtcctcctcc acccaagtgc tgctcagtcc ctgtgagggc
    19561 cttctgctgc atcagatgcc ccagacgcac cttgatcccc tgggaggaga cagctggact
    19621 gtagctgcat ctcatggtcc tgagagtggg ttacagagac ccagaggacc ctgtctgtgg
    19681 ctgggaaact gttggtggct ggtgggcaat aaagacctct tggtatccct aagtgtacga
    19741 ggcgctttac tcctgtgtct ttcaagtgtc tgtttctttc tttctgagga acccctccag
    19801 gctggtttct cccaatctag aaccaagata gtgcaaggat tgagcacagg tttagtgaag
    19861 aaagatggca gctcagaggc tttgcatacc tgaattagat atgccaatca ctagacccag
    19921 ctagaatcaa taagtcagtc agtctgtctg tctgtcttta aaaggacttt tattttttaa
    19981 gaagaatgag tatgggttta gagagatggc tcagaggttg agagcactga ctgctcttcc
    20041 agaagtcctg agttcaattc ccagcaacca catggtggct cacaaccatt ctgtaatggg
    20101 atctgatacc ctcttgtggt gtgtctgaag acagctacag tgtactcata gacactaaaa
    20161 aaaataaaga aaattattta aaaaaataag tatgagtatg tgaatgaaga gcctgcagag
    20221 gccagaagag gatgttggtt cccctggagt tacaagccca acatgggcgc ttggaaccaa
    20281 actggagtcc tctgcaagaa cagtttgcac tcttcccccc ttccccttag gtaaaagttt
    20341 atgtttgatt attaattaaa atataattat attgtgttgc cccttccctt tcctctctta
    20401 acacccctca actcatcatc ctcccttcac ttcctctcaa attcacggcc tctttctttc
    20461 attgttttac acacacacac gcatacacag tgtgcagatt tgtgtttggt tttgctcgca
    20521 tgtaaataat tacagggctg agtacttggt gttgataacc aattaagaat ctcaactctg
    20581 gagaattgtt gttcctcccc cctctgtgct agcatgcctg ttgatgtcct tgctcagatc
    20641 ttgtttaggc agccatattg ttgaacagtt tgcactctta aacaacgagc catctttcca
    20701 tctctctccc tctctcttgt atagatgtgt atgtgtatat ctgcatcggt gaatgccggt
    20761 gcttgtgcat ctatgtgtgc aagtgtgtgc acgccggagg tcaatgttgg atgtctttat
    20821 cgctctctgt cttactacct agtggactct tcttcctgac ggttgagcct acagcataag
    20881 gtctgtgaga taagagtctg tactgaggta cagctccagc cctcacccca ctttttgaaa
    20941 cagtcactca ctaagcctgg agctcatcag tttggcttag ggatccatct ccacagtgct
    21001 gaggttacag aaagaagcac acataaaact tttatatggg tcctagagat ttggactcag
    21061 gtcccatgct tgcataacaa gtgctttccc tgctgagccc tctcctgagc cctcctgttt
    21121 ttctttgtac cacactgttc attgtcacaa ctcacaagaa caaggatttc ctagagctct
    21181 taccataagg aggattagaa cttcatagca ctttagagtg ggatgcagga ttcagcgccc
    21241 tcctgggacc ctgccatgtc ccttaactga cctcctggat acgcttgcaa ggatatgatg
    21301 atgggtcatg taagacctgg ttcaagggct cttgtcactt ctgccatgtc tcgtctatgg
    21361 cctaggtatg actcagagcc agagagaggc tgaaggagct agtcagcgta tcctttgtgg
    21421 gttgttctgt ttgtcttttg tctatccttg cttttctggg tctttataga ggcctggatc
    21481 tcagctgttc tgagggagtg gtggtgagca tctctccctc cccagagtcc catggctagt
    21541 cagagtcaat gtcacaggac caggctgaca gcacaatgag ctttctgtct tctggactcc
    21601 tccttcccac caggttcttc atcactgcgg ttctttccag agcccagtct gccttggctt
    21661 gtggttcttg gcttcctctg tccttggttc ctacacaacc tttccagaat tagaaacaac
    21721 ctgttgttgg gagcatgtga ggaaagggat gggttgagag tcaaaaatgt gggatactaa
    21781 atacaggcca ga
//