LOCUS       KO-first_condition_ready_70143_MGI:1100514        22842 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Pnn targeting project(s): 70143
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 80695
COMMENT     backbone: R3R4_pBR_DTA+_Bsd_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     promoter        399..911
                     /note="PGK promoter"
     CDS             925..1581
                     /note="DTA"
     misc_feature    1706..1959
                     /note="BGH pA"
     promoter        2034..2100
                     /note="EM7 promoter"
     CDS             2101..2499
                     /note="BsdR"
     CDS             complement(4193..5053)
                     /note="AmpR"
     misc_feature    5272..5395
                     /note="R3 gateway site"
     misc_feature    5401..5408
                     /note="AsiSI"
     misc_feature    1..5433
                     /note="Synthetic Backbone"
     exon            8212..8408
                     /db_xref="ENSEMBL:ENSMUSE00000377215"
                     /rank="1"
                     /note="ENSMUSE00000377215"
     exon            9072..9143
                     /db_xref="ENSEMBL:ENSMUSE00000113786"
                     /rank="2"
                     /note="ENSMUSE00000113786"
     exon            9578..9646
                     /db_xref="ENSEMBL:ENSMUSE00000313180"
                     /rank="3"
                     /note="ENSMUSE00000313180"
     exon            10019..10091
                     /db_xref="ENSEMBL:ENSMUSE00000313173"
                     /rank="4"
                     /note="ENSMUSE00000313173"
     exon            10195..10289
                     /db_xref="ENSEMBL:ENSMUSE00000313164"
                     /rank="5"
                     /note="ENSMUSE00000313164"
     exon            10409..10484
                     /db_xref="ENSEMBL:ENSMUSE00000313156"
                     /rank="6"
                     /note="ENSMUSE00000313156"
     misc_feature    5434..11003
                     /note="5 arm"
     misc_feature    11031..11055
                     /note="B1 Gateway"
     misc_feature    11068..11115
                     /note="Frt"
     intron          11116..11990
                     /note="mouse En2 intron"
     misc_feature    11988..11990
                     /note="Splice Acceptor"
     exon            11991..12163
                     /note="mouse En2 exon"
     misc_feature    12175..12768
                     /note="ECMV IRES"
     gene            12769..15829
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    15868..16111
                     /note="SV40 polyadenylation site"
     misc_feature    16115..16148
                     /note="loxP"
     promoter        16175..16694
                     /note="human beta actin promoter"
     gene            16720..17514
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    17714..17955
                     /note="SV40 polyadenylation site"
     misc_feature    17972..18019
                     /note="Frt"
     misc_feature    18026..18059
                     /note="loxP"
     misc_feature    12005..12082
                     /note="FCHK std_gpr"
     misc_feature    18075..18095
                     /note="B2 Gateway"
     misc_feature    11004..18125
                     /note="Synthetic Cassette"
     exon            18495..18650
                     /db_xref="ENSEMBL:ENSMUSE00000113788"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 1 ENSMUSE00000113788"
     exon            18732..18870
                     /db_xref="ENSEMBL:ENSMUSE00000113790"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 2 ENSMUSE00000113790"
     misc_feature    18126..19046
                     /note="Critical Region"
     misc_feature    19070..19103
                     /note="Downstream LoxP"
     misc_feature    19047..19126
                     /note="synthetic loxP region"
     exon            19814..22405
                     /db_xref="ENSEMBL:ENSMUSE00000388385"
                     /rank="9"
                     /note="ENSMUSE00000388385"
     misc_feature    19127..22842
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc
      421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
      961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
     1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
     1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac
     1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt
     1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact
     1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct
     1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat
     1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga
     1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
     1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac
     2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
     2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac
     5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac
     5401 gcgatcgccg gcggataaca atttcacaca ggatgagttg tgtggcctta tattctattt
     5461 agtaccgtca ttaaactgct aagctcaggg acccctctcc tctagagcac tagttctcaa
     5521 ccttcgtaat gatataaccc ttaagtacag ttcttcatgc tgtggggacc tccagccata
     5581 aaattatttt gctactgtta taaattgtaa tgttaatatc tgtgttttcc aatagtctta
     5641 ggcaaccccc gtgaaaggtt gagaaccact gctctaaagt ctacagaagt aagaaatatc
     5701 ctttattctg taggttgccg taggacctcc tatcccagct tcctcaatgg taatgaaagg
     5761 aagaaagaaa gaaaggaaga aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaaga
     5821 aaggaagaaa ggaagaaaga aagagaaagg aagaaagaaa gagaaagaaa gaaagagaag
     5881 aaaagaaaga gaaggataaa ctttaaagaa cataatctgc ctaggcacat ctctccaagg
     5941 aacttggaat cattgtttaa aactaccact acatggattt gcttgttttc ctaataatcc
     6001 cttctaatat ttaaattcat ttatgtcgtt cgtcatataa aggaaaccta aacattccac
     6061 ttaaaagcca cgcatgctgg tatgggtgtg gactcgcaga agttggggaa caaaagcaga
     6121 gactttcagc cgtgaaggga cagatgtaag gcactgaccc actatcactc acagcagcct
     6181 ggaggagttt cataatcttt taaaaatatt tatttttaat tattttatgt gcatgagtat
     6241 ttcacctgca cgtgtatata tgtactcttc gtgtgtctgc tcgccatgaa agcgagaaga
     6301 ggttatcggg ttcccctgac tggagctatc ggtggttgta agctgccctg agggcgatag
     6361 gaaccacact caggccctct tggaaaacag ccagttagat taaccactgg gtcatctctc
     6421 caatgccaaa catttatttt attcctgagt gtgtgtgtgt gtgtgtgtgt gtgtgtgtga
     6481 tatgtattat aaatatataa atagatggga ggggacacct gggttcagtt cccagcatca
     6541 acattaacct tgtctgtaac tccagttcca gtgatcccac cccccacttc tggtctctaa
     6601 gggcatcatg catacatgca tgtagttgca cagataaaca tgagggcaaa atacccatac
     6661 atgcaaaata atagtaaaat ttaaaagatc ctatctttta tgtatagata actcagtgaa
     6721 accttgttta caattagaat taaataattt ttagacttaa ttgcttttta ccaaagaaaa
     6781 aatcttggtt ttctatattg cttcctgcta atatgctata caccttcaag cttcttcaaa
     6841 gatatttcct gtcaccctct ccttttctcc ctttctctct ctccctctcc ccctccccac
     6901 ccttctctct gagggggagt aagtatttcc agacaggatt ctctggctgt cctggaacta
     6961 gctctgtaaa ccaggctaat ccacctgcct caacctgtca tgtgctggga ttaaaaggca
     7021 tgttaccacc tgacttttct cttcttcttc ttcttcttct tcttcttctt cttcttcttc
     7081 ttcttcttct tcttcttctt ctatttcttc ttcttcttct cctcctcctc ctcctcctcc
     7141 tcctcctcct tctcctcctt ctcctcctcc ccttcccccc ttcttctttt tggttttggt
     7201 tttattcaag actgggtttt tctgtgtatc cttggctgtc atggtattcg ttttgtagat
     7261 aacaggtgct gttaacattg ataagataat gaaattcttc ctcacctatc cagttatgag
     7321 cctccacaca aatagcatac aggataagac caagttactg ttactaaaaa caaaacaaaa
     7381 attcttgttc cttttcaggg agaaaacttg taaggatttg acctttgcat ttgtgtaaca
     7441 tttttccgaa ctaaaacgtg tacctctatg tcaagtacaa aaccagcgtc catattggca
     7501 ttgtgggtag ggcaaagtgg aatccagaaa atacacacag gaaaccactg gcaacttctc
     7561 cagaccctct attatacccg caggcattag gccctgctgg aaacaacttt gctcaggatc
     7621 agaatataaa ttgcctcttc atcgagatct gacgacctgt tggacctagg tccgcagcct
     7681 caatttcctt atcattaagc accatctcat tcaagtttct agtttttaac gactaaggca
     7741 cgcaaagcga ttctcagtgt ccacaataac tattagctgt taggatgaag actcctggtg
     7801 gttcagttca ctaatggaag acagcctgcg cctccctgca ctctgggcat gcgcaacagc
     7861 cattatttgc catgcccttt tctgattaaa cctcttgggg aaggaaaaaa aggatgtata
     7921 gcctcttccg tcccatggga agagtttagg cgcactatga agtcagcgac atgtcaagtt
     7981 taagaggatg cctttgcaga cccccgagat tggatctatc acaccgacgt aatccctccc
     8041 cgctctgcgc aagcagcgga gcgcaaccac ggcaggcggc gaggggagga gctgagtgac
     8101 ttgccggctt ctcattggtc cagaatcacg gatcgacaag cttcttcccc ttccggtcct
     8161 cgggtggtcc aggcccgaga agaagcccgc gatcattggc tggggccggc tgtcagtcct
     8221 tccgcgtgga gcggcgggcg cggtgcggcg ctgcgcggcc tggagagcgg tcgcgaagcc
     8281 tgctgcagag agaagatggc ggtcgccgtg agagctttgc aggagcagct agagaaggcc
     8341 aaagagagcc tcaagaatgt ggacgagaat atccgcaagc tcaccgggcg ggacccgaat
     8401 gatgtgaggt acggatcgga cggaacccca gaggttggcg aggtccgggg cctcggggcc
     8461 gggcgcaccc gggacagagg gcaaccggcc gcttgtgggc cgactggggt caaggggccc
     8521 gtttcccggg cggcgggcga gccctctgct cggctaggcg gggagacgcc atgcctgcgc
     8581 ctagggcact gggtgcgagt gcttgcgaat ggaaggaagg cccgcccggc tgcccggcgt
     8641 ggtttccgcg cccccgggaa ggccgagacc acggcttccc gcccttggcg ctgcaggccc
     8701 ggaactgcag tacaaagccc ctttccaccc ctgcggctcc gaacccttgt caagtcggag
     8761 acccccgggg gaagcagtgg ctgcctctcc ctgctggctt gggtgtcctt cccttgaggt
     8821 tggcagatag gacctgaccc agacttatta atatgtagca gtctcgagat acatatattt
     8881 tcgtaacggt ggtcttgggg aagcaagaaa gaggatttta atgtttggtt tcgaagaagt
     8941 ggatctgaaa cggtcatttt agaattattt gtgttcattt ctacgtagtg tgtaccttag
     9001 tgtgtggaag tatatgaagc cctctcatgt gtctgcatag gacttttaca ctgattataa
     9061 ttctttttta ggcccatcca agccagattg ctggcccttt ctggtcctgg tggaggtaga
     9121 ggacgaggta gcttattgct aaggtaagga gatgataact gccttgtttc taataaatgt
     9181 attgcaaaag tagaggttgg agaatattag aacagtttca tatgcactac acttgggagg
     9241 cagaggcagg cggatttctg aattcaaggc cagcctggtc tacaaagtta caaagtgagt
     9301 tccaggacag ccagggctat acagagaaac cctgactggg gggtgggggt ggggggggga
     9361 cagtttcata taaacttaac tgtttttaag ttctgttaag gcttttgaca tctaagaatg
     9421 taattggtgg tagggtgttg ttcatgtaat tgtttgctta agtttgcttc acttgtgttt
     9481 ttcttcttta tgaaatcaac taaattctct agtcgactag aaataccagt ttctgctaat
     9541 gtttctcgag ctttaggttg gatgtttctc attgcaggcg tggattctca gatagtggag
     9601 gaggaccccc agccaaacaa agagacctgg aaggggcagt cagtaggtat ggtttcaaaa
     9661 gatgtctgga gagtcagtac ccatcttgtt ttctgggaca ctacagatat ttctatgtca
     9721 ttgggattga tttgaactgg gctagcccag tttacagaac tcagtgggat tagaagtaac
     9781 acgttctgtt ttgtacttta aaaaacacca agaaacgttt ggatatgaga agagagcaaa
     9841 gcttggtttg gagcatcttg cttccacccc tcccccaccc cccataagaa agtggtaaat
     9901 gcgatataaa ctcagtagaa tatgccttct ggagatgagc atttgaacac ccattcacct
     9961 ttgattgatt atgcatttga ttccatagtg gtcatttcaa ttctttctcc cgtgctaggc
    10021 tgggtgggga gcgtcggaca agaagagaat cacgccagga gagcgaccca gaagacgatg
    10081 atgttaaaaa ggtattgaaa ttaaatatta gaggatgaac acaactctta gtgtgctttg
    10141 aaaagcatga tagcccagaa caagaaatca ttgatccttt cttctctctt acagccagca
    10201 ctgcaatctt ccgttgtagc tacctccaaa gagcgcacgc gcagagacct tatccaggat
    10261 caaaacatgg atgagaaggg gaagcaaagg tgcctgctcg gcgccagggg gagggggtgg
    10321 aaggctcgca ctcagcagac gagacgcttg cttacaggac ctctcagaca gggcatgcac
    10381 acgttcattt tcctcttatg tttcttagga accgacgaat atttggcttg ttgatgggca
    10441 ctcttcagaa atttaaacaa gaatctactg ttgctactga acgggtattg gtgccttttt
    10501 ttttaacttt ttctcttcct tgctttacct catttactca ttaccaaaga ggagattaat
    10561 gttatacagt gcaggtaagg gtgactcaac agagacctgc cttctttgct tctgggtact
    10621 ggaattaaag gtattagcca ccatacctgg ctttatagta ggtttttgcc tttacttgat
    10681 gtgactgggt gtagcatata tatatatgtc tgtacaccct atgtgtacag tgcctttgga
    10741 ggacagaagg gagcccaggt tttttttgtt gttgttgttg tttatttgtt tgtttgttta
    10801 ttttggtttt gagacagggt ttctctgtgt agccctggct gtcctggaac tcactctgta
    10861 gaccaggctg gcctcgaact cagaactctg cctgcctctg cctcccgagt gctgggatta
    10921 aaggcgtgcg ccaccatgcc tggcttggat ttttttaaaa ttacatttat gccgggtagt
    10981 gttttcagta aaatgcctac cataaggcgc ataacgatac cacgatatca acaagtttgt
    11041 acaaaaaagc aggctggcgc cggaaccgaa gttcctattc cgaagttcct attctctaga
    11101 aagtatagga acttcgaacc ctttcccaca ccaccctcca cacttgcccc aaacactgcc
    11161 aactatgtag gaggaagggg ttgggactaa cagaagaacc cgttgtgggg aagctgttgg
    11221 gagggtcact ttatgttctt gcccaaggtc agttgggtgg cctgcttctg atgaggtggt
    11281 cccaaggtct ggggtagaag gtgagaggga caggccacca aggtcagccc ccccccccta
    11341 tcccatagga gccaggtccc tctcctggac aggaagactg aaggggagat gccagagact
    11401 cagtgaagcc tggggtaccc tattggagtc cttcaaggaa acaaacttgg cctcaccagg
    11461 cctcagcctt ggctcctcct gggaactcta ctgcccttgg gatccccttg tagttgtggg
    11521 ttacatagga agggggacgg gattcccctt gactggctag cctactcttt tcttcagtct
    11581 tctccatctc ctctcacctg tctctcgacc ctttccctag gatagacttg gaaaaagata
    11641 aggggagaaa acaaatgcaa acgaggccag aaagattttg gctgggcatt ccttccgcta
    11701 gcttttattg ggatccccta gtttgtgata ggccttttag ctacatctgc caatccatct
    11761 cattttcaca cacacacaca ccactttcct tctggtcagt gggcacatgt ccagcctcaa
    11821 gtttatatca ccacccccaa tgcccaacac ttgtatggcc ttgggcgggt catccccccc
    11881 cccaccccca gtatctgcaa cctcaagcta gcttgggtgc gttggttgtg gataagtagc
    11941 tagactccag caaccagtaa cctctgccct ttctcctcca tgacaaccag gtcccaggtc
    12001 ccgaaaacca aagaagaaga accctaacaa agaggacaag cggcctcgca cagccttcac
    12061 tgctgagcag ctccagaggc tcaaggctga gtttcagacc aacaggtacc tgacagagca
    12121 gcggcgccag agtctggcac aggagctcgg tacccggaag atctggactc tagagaattc
    12181 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
    12241 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
    12301 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
    12361 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
    12421 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
    12481 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
    12541 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
    12601 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
    12661 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg
    12721 gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccat ggaagatccc
    12781 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    12841 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    12901 caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
    12961 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca
    13021 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg
    13081 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
    13141 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg
    13201 gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg
    13261 tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg
    13321 ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt
    13381 ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc
    13441 actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc
    13501 gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc
    13561 agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc
    13621 gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc
    13681 tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc
    13741 gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg
    13801 ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat
    13861 gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc
    13921 tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat
    13981 gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat
    14041 gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt
    14101 aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac
    14161 gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc
    14221 ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa
    14281 gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga
    14341 gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt
    14401 ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg
    14461 gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac
    14521 ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt
    14581 gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag
    14641 ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat
    14701 aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg
    14761 cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg
    14821 gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg
    14881 tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg
    14941 acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc
    15001 atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg
    15061 tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg
    15121 ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa
    15181 cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat
    15241 acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc
    15301 ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt
    15361 gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg
    15421 gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac
    15481 cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg
    15541 tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca
    15601 caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg
    15661 gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt
    15721 ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag
    15781 ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc
    15841 aggggggatc taagctctag ataagtaatg atcataatca gccatatcac atctgtagag
    15901 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    15961 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    16021 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    16081 ctcatcaatg tatcttatca tgtctggatc cggaataact tcgtatagca tacattatac
    16141 gaagttatgt ttaaacggcg cgccccggaa ttcgccttct gcaggagcgt acagaaccca
    16201 gggccctggc acccgtgcag accctggccc accccacctg ggcgctcagt gcccaagaga
    16261 tgtccacacc taggatgtcc cgcggtgggt ggggggcccg agagacgggc aggccggggg
    16321 caggcctggc catgcggggc cgaaccgggc actgcccagc gtgggcgcgg gggccacggc
    16381 gcgcgccccc agcccccggg cccagcaccc caaggcggcc aacgccaaaa ctctccctcc
    16441 tcctcttcct caatctcgct ctcgctcttt ttttttttcg caaaaggagg ggagaggggg
    16501 taaaaaaatg ctgcactgtg cggcgaagcc ggtgagtgag cggcgcgggg ccaatcagcg
    16561 tgcgccgttc cgaaagttgc cttttatggc tcgagcggcc gcggcggcgc cctataaaac
    16621 ccagcggcgc gacgcgccac caccgccgag accgcgtccg ccccgcgagc acagagcctc
    16681 gcctttgccg atcctctaga gtcgagatcc gccgccacca tgattgaaca agatggattg
    16741 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
    16801 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
    16861 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
    16921 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
    16981 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
    17041 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
    17101 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
    17161 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
    17221 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
    17281 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
    17341 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
    17401 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
    17461 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
    17521 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
    17581 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    17641 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccccg gatctaagct
    17701 ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa
    17761 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
    17821 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    17881 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    17941 atcatgtctg gatccggggg taccgcgtcg agaagttcct attccgaagt tcctattctc
    18001 tagaaagtat aggaacttcg tcgagataac ttcgtatagc atacattata cgaagttatg
    18061 tcgagatatc tagacccagc tttcttgtac aaagtggttg atatctctat agtcgcagta
    18121 ggcggacttg tatgttggta aggtggagac agatggattc ctagagctca gcaaaggcct
    18181 aatacagtga gccatcttga aaacctaggc taaattgtat taaggaagct tttatgattg
    18241 ctgactagtg actttaaaat ttagatattt gtggggaaat gcctcagtta ctatttccca
    18301 aaacacagat tgcaatcaga cccaaataca aagttaaaat taaatgggaa gaaacgtgat
    18361 gaaagcctac gagtaaattt acatagtaaa atttaagttt caaataagca agatgtgtgt
    18421 ggcctttatt gtaattagat taaaactaaa tgaaccttgg ttgtcttata ctgaagaaga
    18481 ttacttactt ttagcaaaaa agacgccaag aaattgaaca aaaacttgaa gttcaggctg
    18541 aagaagagag aaagcaggtt gaaaacgaga ggcgagaact gtttgaggag agacgtgcta
    18601 agcagacgga gctgcggctt ctggagcaga aggttgagct tgcgcagctg gtgagcaggg
    18661 ttttccaccc gaagtgaggc aattaattgt tcatgtttac acattgttca tggtcacctt
    18721 tttaccttta gcaagaagaa tggaatgaac ataatgccaa gataattaag tatataagga
    18781 ccaagacaaa gccccatttg ttctatattc ctggaagaat gtgtccagct acccaaaagc
    18841 taatagaaga gtcacagaga aagatgaatg gtacgtatgt gaagtccatt ggtgtgtctt
    18901 agtggtaaga ctatacattc acatatgccc gtttttaagg aataatttaa tagtttagtt
    18961 tgggtttttt tttttttttg tatgtgattt ctgaaattga tagtggtgtc agggttcagg
    19021 ggtattttct gggtagttga gtttaagaga tggcgcaacg caattaatga taacttcgta
    19081 tagcatacat tatacgaagt tatggtctga gctcgccatc agttcatgtg tgttggggct
    19141 ggagagatgg ctcagcagtt aagagcactg acagttcttc caaaggtcct gagttcaatt
    19201 cccagcaacc acatgatggc tcacaaccat ctgtaatggg atctgatgcc ctcttctagt
    19261 gtgtctgagg acagtgacag tgtactcaca taaaataaat aatcaaatct ttttttaaaa
    19321 aagacttttt cttattaagg atttttgtgt tttacaatcc gagcagacac agaagaatga
    19381 taaggaaaga aggcaaaaga agcataagtc catgtcagat attcacatag agggctagac
    19441 gctggtgatt ggacattttg ctgtgcctgg gaacagaaaa gttaactaag tagtgcttcc
    19501 gctttaacgg ggttgtgtta agcttcacct agcataaagc tcagcagctg aggtatccct
    19561 ggtgtcgctc aggtgtttgt ccaggtttgt gtgagtagaa tccctaggtc tggaggtgtc
    19621 ttgcggatat ttgcatttag accttgagtt tagttctcaa catactcccc gaccctaaaa
    19681 caaaccaaag caaaacctag aggtacaaaa agatgcttag ttgacagtaa ctatttttga
    19741 cctggtataa aattgttatt ttgtactttc ttaactgtca taaactttac acgtgtaaat
    19801 tttttttgtt tagctttatt tgaaggtaga cgcatcgaat ttgcagaaca aataaataaa
    19861 atggaggcta ggcctagaag acaatcaatg aaggaaaaag agcatcaggt ggtgcgtaat
    19921 gaagaacaga aggcggaaca agaagagggt aaggtggctc agcgagagga agagttggag
    19981 gagacaggta atcagcacaa tgatgtagaa gtagaggagg caggagagga agaggagaag
    20041 gaagcaggta tagttcatag tgatgcagag aaagaacagg aggaggagga acaaaagcag
    20101 gaaatggagg ttaagactga ggaggaggct gaggtaagag aaggtgagaa gcagcaggat
    20161 agtcagccag aagaagtgat ggatgtgcta gagatggtgg aaagtgtcaa acacgtaatt
    20221 gctgagcagg aagtaatgga aactaaccaa gttgaaagta tagagccttc agaaaatgaa
    20281 actagcaaag aattggagcc agagatggaa tttgatgttg agccagataa agaatgtaaa
    20341 tctctttctc ctggaaaaga gaatattaat tctcaggaag tggaaaagga gtctgaggag
    20401 aaagaggaaa aagaagagaa agaacctgag ccccaacctg agcctgtggc tcagcctcag
    20461 cccccacctc agcccctgcc ccagagccag ccccattctc agccccactc ccagccccag
    20521 cctgtactcc agtcccagcc tctctgtcag cctgagactt tgccattagc tgttttgcag
    20581 cctccacctc aggttattca ggagcaaggg aatctactac ctgagcggaa ggattttcct
    20641 ctagagtcta taaaactccc tgaggtgtct gtagagccag tcttgacagt acattcagaa
    20701 aacaaaagca agaataagac taggagcagg agcagggggc gagcaaggaa taaaacaagc
    20761 aagagtagaa gtcggagcag cagcagcagt agttccagta gcagctctac tagtagcagc
    20821 agcgggagca gctccagcag tggcagcagc agtagtcgaa gcagctccag cagcagctcc
    20881 agcacgagtg ggagcagcag cagagacagc agcagcagca ctagcagtag cagtgagagt
    20941 aggagtcgca gtaggggccg aggacataac cgagatagga agcacagaag gagcgtggac
    21001 cggaagcgaa gggatacttc aggactagaa agaagtcaca agtcttcaaa aggtggtagt
    21061 agtagagata caaaaggatc aaaggataag agttcccgcc ctgacagaaa aaggtctata
    21121 tcagagagta gtcgatcagg caaaagatct tcaagaagtg aaagagaccg aaaatcagac
    21181 aggaaagaca aaaggcgtta atgaagaagc caggcttgct tagcctattc tttgcagcag
    21241 aagatgtctt gatgagtaaa aggattacct tgtaaagagg atgctgcctt aagaattgca
    21301 tgttgtaaaa attctttttg gagaatacag actgtttgtt taccagacat tcttgtactt
    21361 tttgcataat tttgtaagag ttatttatca aaattatgtg aggttccaaa atatgtaaaa
    21421 atgataataa taaaaaaaga ttaacatcct ttgtcatctt ttttaactac cctattactg
    21481 ttcagtcaga atccgtatat tttaataggt aaatctttaa attgttcctt atgttctgta
    21541 tcatacattg cttttgtaga aataaatatg catctttcat tatttttggg atgtccttgt
    21601 tgacacttgt ataataaata tctttatatc attttcagtt tttgtcacaa gatactgaac
    21661 atgattagga tgtttttgaa ggtttcatca ctttgatttg cctgaaaaca gttgccaggt
    21721 ttttacagct ttgacaggga atagttcttt taaaacattt atttcctatc ttacagtttt
    21781 taatctaagc atttttctgt ttttcattaa aaccattcag ttggtagata acttcaaccg
    21841 tacgatttgg ttgtcatttc acgattatag aacaacattc tcttgtcctc tcagctcctt
    21901 gtgtgataac aacttagagt gtaatctgtt atagttatca cctcccgggt ggtgggggtc
    21961 gggtaaaaat ctcttctaaa cttggttttt gaatctgtgg tcttgtcttt tctgttggct
    22021 ataactgaaa catgccaatc tgtgttttca agaatttttg tttaaggaag tattgtatgg
    22081 aagtttacac aatgaaggaa gttcatctag acttgaatat gtaaacaaga taacacacaa
    22141 gtgtaccaag tgattattaa ctttgttgtt tataaatttg tatggacctg gagtatctgt
    22201 tgcccattac tatatatgtg caaataaatg tgacttagac ttgtgactgt ttaaaattag
    22261 tacttgtatt aaagttttga tgcatatgag agcatttaaa tgacagtggc tattttaaca
    22321 ttgtagtttt agcctgatat taaatattca gtatattgtc tataagtaaa tggtttcagt
    22381 gaaaaatgaa caaatatctt catttagttt tgtgtctgaa ccactcatgt gttaaagcaa
    22441 atgtgaaaaa gaattatttt taaaaatcct tttcttggat taagtgaacc acttacatcc
    22501 aaaagcatgc tgatgccaag acgggcttaa ccaacggctt gtacttggca aaatgctttg
    22561 gccttgtgct atgcaatgag acagattggt aaaggactga ctagattaga aatgacagga
    22621 gccctcccct agcacatagt gggaggggga ctccaagaaa ggagtactca ataacatgtt
    22681 ttacttaaat gttttgtctg tgggcgtgtg caggcaggca ccaaggcagc cagaagacat
    22741 tatatccctc agagtgagag tgaggcattg ttcattgtgg aggctgagga acaaacaggt
    22801 cctcctaaga tgtgtattac cacaaccacc ctaaaaaatt tt
//