LOCUS       KO-first_condition_ready_79670_MGI:2178217        22855 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Akap9 targeting project(s): 79670
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 37213
COMMENT     backbone: R3R4_pBR_DTA+_Bsd_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     promoter        399..911
                     /note="PGK promoter"
     CDS             925..1581
                     /note="DTA"
     misc_feature    1706..1959
                     /note="BGH pA"
     promoter        2034..2100
                     /note="EM7 promoter"
     CDS             2101..2499
                     /note="BsdR"
     CDS             complement(4193..5053)
                     /note="AmpR"
     misc_feature    5272..5395
                     /note="R3 gateway site"
     misc_feature    5401..5408
                     /note="AsiSI"
     misc_feature    1..5433
                     /note="Synthetic Backbone"
     misc_feature    5434..10243
                     /note="5 arm"
     misc_feature    10271..10295
                     /note="B1 Gateway"
     misc_feature    10308..10355
                     /note="Frt"
     intron          10356..11230
                     /note="mouse En2 intron"
     misc_feature    11228..11230
                     /note="Splice Acceptor"
     exon            11231..11403
                     /note="mouse En2 exon"
     misc_feature    11415..12008
                     /note="ECMV IRES"
     gene            12009..15069
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    15108..15351
                     /note="SV40 polyadenylation site"
     misc_feature    15355..15388
                     /note="loxP"
     promoter        15415..15934
                     /note="human beta actin promoter"
     gene            15960..16754
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16954..17195
                     /note="SV40 polyadenylation site"
     misc_feature    17212..17259
                     /note="Frt"
     misc_feature    17266..17299
                     /note="loxP"
     misc_feature    11245..11322
                     /note="FCHK std_gpr"
     misc_feature    17315..17335
                     /note="B2 Gateway"
     misc_feature    10244..17365
                     /note="Synthetic Cassette"
     exon            17786..17999
                     /db_xref="ENSEMBL:ENSMUSE00000505652"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 1 ENSMUSE00000505652"
     misc_feature    17366..18202
                     /note="Critical Region"
     misc_feature    18226..18259
                     /note="Downstream LoxP"
     misc_feature    18203..18282
                     /note="synthetic loxP region"
     exon            19869..19942
                     /db_xref="ENSEMBL:ENSMUSE00000503624"
                     /rank="9"
                     /note="ENSMUSE00000503624"
     exon            21107..21242
                     /db_xref="ENSEMBL:ENSMUSE00000381275"
                     /rank="10"
                     /note="ENSMUSE00000381275"
     exon            21961..22046
                     /db_xref="ENSEMBL:ENSMUSE00001224598"
                     /rank="11"
                     /note="ENSMUSE00001224598"
     misc_feature    18283..22855
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc
      421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
      961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
     1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
     1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac
     1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt
     1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact
     1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct
     1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat
     1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga
     1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
     1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac
     2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
     2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac
     5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac
     5401 gcgatcgccg gcggataaca atttcacaca ggattgagag acttggtctc attttccatt
     5461 ataattactt tgctttagta tctattatct ttttagagta tttcatactt gtgttttcta
     5521 gtataaaatt tcaagttttc agttagtatc ccacaactgt aaaacaaaac aaaaaacctc
     5581 ctaagttttc tactgctctg taaagtactc agttgttatg aaattatatt tctgagaggt
     5641 gaggcttaag gccttaagtt tttgagtctt atacttgaat gagaaaaaaa taccgttaat
     5701 tttcaatata agtcaagatc ttaacaatac ctataataaa tgttctagtt tcatcctgtt
     5761 gctgtggtaa agctgaccag aagcagctta ggagaggaaa gtgttgtgat tggcttacac
     5821 gtcctggtca cagtgaagtg agggtgaaaa ctcagtccga acaccataga ggagtggcgc
     5881 ctcctggctt acccactggc tcatgcttgc ctacctttct ggtatagctt gggacttcct
     5941 gcctacataa tggtgctgtc cacaggggct cccacatcaa ttaacaatca agatgttcac
     6001 ccatatatgc ccactgacca gtctaatctg agcagtattt gaattgagac ctttctcatc
     6061 tctttgttga ttctagactg ttgtcagtaa tgctaacaaa gaccgtgact aataatgagt
     6121 cattcctaat aacttatatt tggtaaatac taatgctaga aaaaccagga cagttctggt
     6181 aggttatcta cagtataata atatgtgttt ttatattagt ggaatatatg tttattgaat
     6241 tattctagat acacatatag tggtgaacaa gagatgagga ctagtctcag gaagcttgct
     6301 ctcaagggga aagcagttag taagggggaa gcaaagggta gttagaacca gtgtcaggtc
     6361 tgtgaagaag tacagggcta ggcaggagag tacagagatc aggacaggtc ttttggagga
     6421 gttagccttt gaactgagct ctgtataatg gggaatttat agcagttgca aagatgtggg
     6481 tgagcgttag gatccaggcc tatgtggttg gctctgaaaa gaggccaaag gccaaacaga
     6541 gctgtggaag gatggaagga tgcaggaagc tcagttttca ttctaagtac aagccagggg
     6601 atgtttgtct ttatttctga tgacttaatc ccattgacaa tcttatatca aatacagaga
     6661 gaatttgctt ttataataac ttataataac ctgaatagga tcaagaaatt caggctggag
     6721 agatggctta gcggttaaga gtgttggttg gttcaagagg gccccagttc aattcccagt
     6781 gcccacatgg catctcacaa ctgtctaact ccagttccag gggatctaac accctcacac
     6841 agacatactg caggcaaaac atcaatgctc ataaaaataa ataatatttt taaaagaatc
     6901 aagtaattca aattactaat taatgtctaa acacatggaa ttgttttgaa aaatatgtgt
     6961 tataattacc ccaaaggtgt tcaacaacat tttaattttt ttacataagg gaggatctga
     7021 tctccaacgt tagctgctct gctcttgata ctgtctctca ctaaaattaa ataaatagag
     7081 ccacataggc tttcttttca gtggaaacaa aatacacttg aagtgtgact tctttatttg
     7141 aatatacttg gataaaacga gaaagagaaa cttcacagaa agtctcccta gtgttggcat
     7201 gtgaaggtct ccagggctgt ggccatgcgg aggcagacag ggcatgacag gaattgctca
     7261 tttcttatgc tcgcctagtg aagcatgttt gaagtggaag acacggaact tagtgagccc
     7321 aggaggagct gccagtttgc tgtctctcag gtcctgccct gtgggcaaga cctcaggaac
     7381 ctagagtaaa tttgatacaa gatagtacat ttttaagcag gaaaataaag cccatttttc
     7441 aagtgtcaat agtggaacaa agtagaactc ctcacaggaa agaagagact ttgttagttg
     7501 ctttatagca ttttccaaaa gtcctaacca ttgtaaatat aaaaagatat acgacatcag
     7561 tggagagaaa gacaactgtc ataattcaca gggacaattt aataagaaag taagcaaatt
     7621 gagttaaaaa atttgaagta aaatagtgta aaaaaaaacc ccaaaaatgt gtaatatacc
     7681 tgggtgtggt agcttatgcc cataatccca ggacacagaa gcagacagag ggaactgtga
     7741 attcaagagc agactcaggc atgtagcaag acctcatcct aaaatctaaa cagtgacagc
     7801 tgcgttgtac gtgaatgaag gagggtgagg cctgaataaa cgaaatctct cacttctgga
     7861 ggaaaatatt taatgtgaga tataagtttg ttttatgtgt ctgcaatcca gttcacagtg
     7921 aaaacttgct aagctggttg gaaagataaa atagcatcca agtagccaca cttagcgaaa
     7981 gcagatagaa gaaaatagaa aatttagagc agaaataagt gaatttgaaa taaccaaaag
     8041 ggaagggttt gaagacactt gatggtttat tatttgaaaa catctatagg ttgcagttgt
     8101 tagattccca aggatgtcac caagaaagag aaagcacaga taaaatgaag gatggaaata
     8161 aaatccccag cgcaaatgct acaaggattt taaatataaa aatactataa ataaggccta
     8221 ctgatgaaaa atataagttg cccaaactaa ttgcatagaa atgcaaagct ttagtgagct
     8281 aatggcacta cagaatctag aactgtggtc ggcttccctt ctcacctctc catgtaaatg
     8341 tgctctgctg tgaccactgt gcagtgtgcg gcggctctca ggagctcacg gctgagcgca
     8401 gagccttact gaggtggcat taaaagagaa ttggagggat ggaaggattc aagctttgag
     8461 tctggttggt attgagataa agatgggaaa agaatggctc aagaaaaaga aaattacaga
     8521 ttattcttat ttatgaatga gcctgcctga attcttatta agttattatg agattatccc
     8581 taagaatgga agaattattc ggcatgtgat aattctgttt actcaatctc cacatgctga
     8641 tcatcttaaa tactgttgga aaagaatctc caaaagttac caatttgttt ccaggtttta
     8701 acaggaataa caagaaggaa agaaactttg aagaagtaag gactagaagg agcagttgtg
     8761 aaggttttct gaggtgttgt tgagtagctt tcccggtgta ggcacggcag ggacagggca
     8821 gtcactgtca ccgttactct tccaacagtg cttctagagt tcctagccag ccaatacaat
     8881 acaaataaga aaggagaaag actgtgaaca agtaagttaa aaatgttaaa ttatagataa
     8941 gattattgaa acttacacaa gacattaatg acctgtaccc gttgccacag gctctcttta
     9001 tacactttag tgtaaaggga taccccaaag gtctgtacct ccttctgtct ccatctccat
     9061 ctttatctct ctctgtctct gtctctcgta cagagttagt tcatgggagg gtattctggt
     9121 gagataatat ttgagaagag acttgaatca acaagccaag acaatgggaa ggctcagaaa
     9181 attcaatgat tgccttaaga gaattgtttt tgcgatgggg aattatatcc ttactcttag
     9241 gaaaaattaa ctccaggtta gttaaagaca tactagacag aaggatgtaa ataaagtaga
     9301 attgtgatct taagacgtag gaagcctttt gattaagaag agcaaagtca aaggaaagta
     9361 tttagaaatt tgatcatgtt tgtttccttt atgaacaata cttaaaacat gtttcatact
     9421 agggccagat gtttgccaca tatgttacaa acaaaggact gtaaatgtaa tataaatacc
     9481 tgagaaaata caaaggattt aatgagccaa agatatgaag aaatatgtgt cttagagaag
     9541 aaaccaaaat gacaaggaac ataaaaagta ttttagcctt acaaattcat atgagtaaaa
     9601 tagcatttta tcaccatcac agtggcaaaa taaccgactt attgataagg ctacaaggat
     9661 gtaagtatcc actttttttt agttgaatta tacaaaacaa ttatttagct actaaagcta
     9721 agcctgcgct atgaccagca gttacattcc tgagtaactg tggtcagtgg gaagcagcta
     9781 cgcttggaaa caggcagcac tcgtgtttct taagcttcct gaatcagggt cagaggacag
     9841 ataccataga tgtgtgtcag cgagaattcc agttgagtct gatacatgct ggaccgctga
     9901 cgagcagagc ctttactcat ttgtctcagg tggatgtatg taatcattta ctaccttttg
     9961 attaaaggac ccaggcttat gaaaatccag ttgggctccg caaaggaaaa cggtccagtt
    10021 gtggattgtt catttatact acactatagt caaaataaat taacctgtca catatgtgga
    10081 ttaatttcag aagcattttt tgatgttttg atatttgata ctaaaatgca aagaaatttg
    10141 caaaataaat gcctctgtgt caatacacag aaatatacaa tataatgtaa tgtttacatg
    10201 ttcactggga aatgatagta aacaccttcc agatttttgg taaaaggcgc ataacgatac
    10261 cacgatatca acaagtttgt acaaaaaagc aggctggcgc cggaaccgaa gttcctattc
    10321 cgaagttcct attctctaga aagtatagga acttcgaacc ctttcccaca ccaccctcca
    10381 cacttgcccc aaacactgcc aactatgtag gaggaagggg ttgggactaa cagaagaacc
    10441 cgttgtgggg aagctgttgg gagggtcact ttatgttctt gcccaaggtc agttgggtgg
    10501 cctgcttctg atgaggtggt cccaaggtct ggggtagaag gtgagaggga caggccacca
    10561 aggtcagccc ccccccccta tcccatagga gccaggtccc tctcctggac aggaagactg
    10621 aaggggagat gccagagact cagtgaagcc tggggtaccc tattggagtc cttcaaggaa
    10681 acaaacttgg cctcaccagg cctcagcctt ggctcctcct gggaactcta ctgcccttgg
    10741 gatccccttg tagttgtggg ttacatagga agggggacgg gattcccctt gactggctag
    10801 cctactcttt tcttcagtct tctccatctc ctctcacctg tctctcgacc ctttccctag
    10861 gatagacttg gaaaaagata aggggagaaa acaaatgcaa acgaggccag aaagattttg
    10921 gctgggcatt ccttccgcta gcttttattg ggatccccta gtttgtgata ggccttttag
    10981 ctacatctgc caatccatct cattttcaca cacacacaca ccactttcct tctggtcagt
    11041 gggcacatgt ccagcctcaa gtttatatca ccacccccaa tgcccaacac ttgtatggcc
    11101 ttgggcgggt catccccccc cccaccccca gtatctgcaa cctcaagcta gcttgggtgc
    11161 gttggttgtg gataagtagc tagactccag caaccagtaa cctctgccct ttctcctcca
    11221 tgacaaccag gtcccaggtc ccgaaaacca aagaagaaga accctaacaa agaggacaag
    11281 cggcctcgca cagccttcac tgctgagcag ctccagaggc tcaaggctga gtttcagacc
    11341 aacaggtacc tgacagagca gcggcgccag agtctggcac aggagctcgg tacccggaag
    11401 atctggactc tagagaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa
    11461 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
    11521 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
    11581 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
    11641 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
    11701 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
    11761 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
    11821 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
    11881 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg
    11941 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg
    12001 ccacaaccat ggaagatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
    12061 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    12121 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct
    12181 ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg
    12241 atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca
    12301 acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt
    12361 gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta
    12421 tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg
    12481 gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa
    12541 accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata
    12601 tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa
    12661 tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg
    12721 ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc
    12781 agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc
    12841 gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga
    12901 gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc
    12961 tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc
    13021 tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc
    13081 tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga
    13141 acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt
    13201 gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc
    13261 caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc
    13321 gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat
    13381 caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc
    13441 gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga
    13501 tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa
    13561 aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga
    13621 tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt
    13681 tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg
    13741 gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct
    13801 gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac
    13861 accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat
    13921 acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc
    13981 cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac
    14041 tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc
    14101 aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc
    14161 tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca
    14221 ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt
    14281 caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg
    14341 atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg
    14401 accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt
    14461 tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt
    14521 ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg
    14581 gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga
    14641 ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc
    14701 cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat
    14761 tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc
    14821 gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct
    14881 acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca
    14941 catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt
    15001 cagtatcggc ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc
    15061 aaaaataata ataaccgggc aggggggatc taagctctag ataagtaatg atcataatca
    15121 gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    15181 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    15241 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    15301 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggaataact
    15361 tcgtatagca tacattatac gaagttatgt ttaaacggcg cgccccggaa ttcgccttct
    15421 gcaggagcgt acagaaccca gggccctggc acccgtgcag accctggccc accccacctg
    15481 ggcgctcagt gcccaagaga tgtccacacc taggatgtcc cgcggtgggt ggggggcccg
    15541 agagacgggc aggccggggg caggcctggc catgcggggc cgaaccgggc actgcccagc
    15601 gtgggcgcgg gggccacggc gcgcgccccc agcccccggg cccagcaccc caaggcggcc
    15661 aacgccaaaa ctctccctcc tcctcttcct caatctcgct ctcgctcttt ttttttttcg
    15721 caaaaggagg ggagaggggg taaaaaaatg ctgcactgtg cggcgaagcc ggtgagtgag
    15781 cggcgcgggg ccaatcagcg tgcgccgttc cgaaagttgc cttttatggc tcgagcggcc
    15841 gcggcggcgc cctataaaac ccagcggcgc gacgcgccac caccgccgag accgcgtccg
    15901 ccccgcgagc acagagcctc gcctttgccg atcctctaga gtcgagatcc gccgccacca
    15961 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
    16021 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
    16081 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
    16141 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
    16201 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
    16261 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
    16321 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
    16381 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
    16441 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
    16501 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
    16561 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
    16621 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
    16681 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
    16741 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
    16801 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    16861 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    16921 ccaccccccg gatctaagct ctagataagt aatgatcata atcagccata tcacatctgt
    16981 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    17041 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    17101 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    17161 caaactcatc aatgtatctt atcatgtctg gatccggggg taccgcgtcg agaagttcct
    17221 attccgaagt tcctattctc tagaaagtat aggaacttcg tcgagataac ttcgtatagc
    17281 atacattata cgaagttatg tcgagatatc tagacccagc tttcttgtac aaagtggttg
    17341 atatctctat agtcgcagta ggcggaatta aatcttgtct ctaatgtttt ctttctaaat
    17401 ctaaggagct gtagatggaa taggagaaaa aaaaatgaca gcattgagaa ttattaattt
    17461 gagttttgag ggcattggtg tttattcaga ttatttttat tttgtagcat gtaaaatatt
    17521 agttaaaaga ttaaattttt gctagttata ccaactatcc caacttacaa ttacactgag
    17581 ttagtaaatg aaaacatgac aaggcctttc ttgagaaaga catgtaggtc gggcatctgg
    17641 atgaagacta taaagcaggc cagttccttc ctatgacttt taaaactatg acatgtcttc
    17701 tcctttgaag tccagtccat ggaaagaagc agaagctaag ctgttacttt cattttccct
    17761 cacgtttgta cctcttccct ttaaggatga tctaaggctg cagatggagg cccagcgcat
    17821 atgccttttt gtggtttact caactcatgc ggatcaggtt cgtgcacaca tggaaaagga
    17881 gagggaggaa gctctttgca gccttaaaga tgagctcatt tccgctcaac agaaaaagat
    17941 cgacgaactt cataaaatgc accagtgcca gctccagaat tttaaaatac aggagacagg
    18001 taacccatcg gcttctgact aatttctatg aaacaaataa gcacccttaa aggcaccttt
    18061 ttgtagttct gtccctgtag ttctggcatg ggctgaggag gctttgcgtc tgttgcccgg
    18121 gtttgttaat tcatgtcatt gaggtgccag gatagccaaa ccccttcctg cctcattttc
    18181 tcacggaagt ataatccatt cagagatggc gcaacgcaat taatgataac ttcgtatagc
    18241 atacattata cgaagttatg gtctgagctc gccatcagtt catctgtctg gggcagatgt
    18301 acaccattgg ctctactgtt acttgaagta ttccagacta acgaataatc cagctgatcc
    18361 agggtgaata ctgagagtgg aaggaagagg agaggagaag aagttctgtg aagatggagc
    18421 aggatcagtt gggttgggca ctgagggatg tttctgaagt gttaagcata gacctgaaag
    18481 agacccactt tggagaagtg aaagttcaag ttgtatagaa agtagtgggg catgagctga
    18541 agattgtggc gcgtgcctgg ttagaagctt cataaatctc aacggagtac actcatgctt
    18601 cctgctctgg gaagacagag ctactcgatc tactgcaggg tttgtaagga gagcaagcaa
    18661 cagtgacact cttatttaag aggacactag tgcgcttagt tgagaaaagt gaaataataa
    18721 tgggcagagc agctaagcaa tcttagctat aaccaataaa tgtctggaaa cacagtgaca
    18781 gtgaagctca acagttagag ctaaatgcta tcacggggca atttatagca gcaggtggta
    18841 gaagttgaga ataatcttga agtttctgcc tatgtagttt cagtcacaaa gaggagcaga
    18901 ccatagacaa ataatatagt cagtttagga cattctgagt taagtgtatg tgttaacttg
    18961 taaaagaaat agtcactagt cagctgaagg gagtagtcta aaacccagga gagactttta
    19021 ggctaaaaat acgatctaca tggagctgaa agaccagagt ctctaaggcc agaagtagag
    19081 tgagacaccc tggagatgga gcagaaaacc ttggtggcgc ggtgggaagg acattgaaga
    19141 gaccacagag gaaggcagaa cctgttatgg ggcagctgtt gttgaggaaa cccagtgttt
    19201 cctaaatgtc cagtggaatt gctctgttga acaaagttaa gatttcaaga gtcacttaaa
    19261 gaaggagagt ttttcattta cagagttctt tgcttttttt ttctaaattc attaagattt
    19321 tgcatgcatt gcttgtattg atacctcttc tgatagcctg tctagtgttt tctattgaca
    19381 gtcgattgtt ctattactag gtgacaagac agtctagtga gtagtgatca atgtctctga
    19441 gaagagaaga gaacagaaag tatatatcta ataaataact aatggattta atgttgcaat
    19501 actaggactg aaatccaggg ctagcacata ctaatcaaag tgtcctatct ctgaactctg
    19561 actctgcaaa attggaacca ctggcttgtg agagttttga ggtttgcaaa ggtgtattta
    19621 atggataagt aagtatatag gttcttgggt tagaatgtag aatcctgaat gtataaaccc
    19681 ttctatagag tcgaaatagc aaatcttaga aatctatctg agacagctgc attgcaaaat
    19741 gaaaaacaca tttggaagaa attgaaatat gtgatattct agggaagctc cggtgaaggc
    19801 attagaatgt gacagtgtta cttacatata tgcagcaaat ggctcacgtg tggtgtgttt
    19861 cctgctaggt gatgagcctt tacaggtgct cattgaacga cttcaacagg cagtgtctga
    19921 gaaatgcttt cacatttcaa aggtaagatg ttcctttaaa agtggtttta gttgggaggt
    19981 accaaaatcc atattgctac tgatttaatg aacagaacca tccactcacg tttgcccaaa
    20041 tctcttgcgt ccagaactgt acttacaaag tattcctgag ttctaagctt aaaatgagca
    20101 tttgcctttt ttgtgatagt cagattggaa caataggaaa ttagagataa gggtgtgact
    20161 ctgatccatt gcttgtctgt tatatttgag gtcttggatt cagtctctgg caccacagaa
    20221 aaagaacaaa gaagttgaga taagttgtat ctcgaatagt gcacactgta tagtcactgt
    20281 tcgtgtcaga gaggtactgt ttaaccttcc aggaaaacag tttgttcatt aaggtcacta
    20341 atgtggtaaa agtttacctt gtggctgtgc tgaaatctca gtgtgcccaa atactgacag
    20401 tgtccttaac ttaaaatcat gcaggaagca acagccattt aaaacattgg cttttaaaag
    20461 tcttctaaat cattggttat ttccaattga gagaggggca aaggtaaagt taacttggac
    20521 agcttatcaa accttggaac atgaaatggt gtcttacatg atggaaattt cagtttctct
    20581 ggagaacagt gtgctggaca cttttaactt tttgtatggg cagaccacag aaaacactct
    20641 gcatattata gaaaggacat tagtataatc tcctcctcaa tatattatca ttttggttgg
    20701 tttgatagag ctctctaatt gtcctctttg agctttctct ccttgtgatg tttgttcaag
    20761 gatagttggt gaagtaatga gatattttca agggagagca attatatcag agtgggaact
    20821 gtgctagggc tagggagtga ttgcttacac ttcatgatat atttcagaat taaaagaaaa
    20881 cagaaaaatc tgtactattt tgtatagtta agattatctt cctgataaaa gaataatttc
    20941 atgtcaaaga gaaataatgt tttctctgta gattgatttc atatgtggag ggagagggaa
    21001 tccatattcc attcatacta ctatgacctt ttgcctttag gttaattttt ctaatattta
    21061 gaaaaaaatt aaaattttaa tgtaaaatat tcaattttgt taacagaccc taaacaatgt
    21121 tttcgatgaa tgctatactc ctttaaaatg tgaaatgaat atagaagaga aagagaattc
    21181 tggtgtttac acttctcaaa atcaaagccc agaattacaa gagtatagat acgaagttca
    21241 aggtaaaaag cttactatac tgttaaacag gatttacttt gctcaagtca attgcttata
    21301 aacgtaactt taagcaactt aatgatagat acgttttctt agtatcttat ttttagccat
    21361 gaaattcata tttctttctc cttgatttct tcattccaat ctatttttta ttctaattct
    21421 ttttgttaac tgtgctaact gtagatctta atctttaaaa tttagagttt actaagttta
    21481 aattaatggt ttataatttt aaaagtggat taagaaattg agttttaaga tacattaaga
    21541 aatgacctct gccagctgtg gtagtgcatg tatgcaggcc tttaatcccc acgctcaggt
    21601 ggcagaggca gaggcatctg tgtaagtcct tggcccgctg agtctgcacc gctgtttgag
    21661 gctgaccagg gcagggctaa atatagaaag agataatttt cttcatgtaa atgtggtgct
    21721 agttttgaat ttattggtgt tccttaacag atttattatg ttcctagtat atttgtatag
    21781 gtaacgagca acctgctaat aaacagctac agaaactaat acatcttaag ataaaatttt
    21841 caagataatg tcataccagt tacttcatag taaagttcta agtaggtaaa tcttgttttg
    21901 taaaagaggt aaacttgtac agtgaaatga aatgattatc atggtctgtt cttccttcag
    21961 actttcaaga gagcatgcaa gtccttcttg gtaaagtgac ggaagaatgc cgcaaactct
    22021 caggactgca gacgcgctta ggcaaggtct gtgggataga ggagatgata tctgcatttg
    22081 ttttactgcc tgctgttgtg tcaatcagga agttctaaaa ttggtgaacg tggagaagga
    22141 tgctagaaat attaaatcat ttatatgttt gtattagaac atctatatct taagctgaag
    22201 aaacttccag aatttaaatg gtatgtaact tgctgagacg agtttttaag tcatgatgaa
    22261 gtgtaagtgg atgtcaaatg ttaaagtatg aaacaattgt atttagttgt atagatgtct
    22321 ttccttttta gcttactgga gcttaaacac acagtagaac agtaacctat cactaaacta
    22381 cacctgtagg ctcttacatg tagtatttta aggcaatgcg tctgtcttta tatgttaaag
    22441 acgataatta gttgttttgc acatcactga tatcacattt aacatttaac tctgtctgtg
    22501 tacttttgtt gatgtgccct ctgatgggcc acgtttctct gccccactgc cttctttgcg
    22561 ggtgctggct ttttctgtgg ttcagccagc accaaccgtt ggctctgccc cagcccttca
    22621 gtccaatact ggctacagca ttgtgtctga tcttactaga gtcacttgac acacttcaat
    22681 ttttcctgta tgagtggaag atcatgctca gtttgtgtgc caatggcatt cacctgagcg
    22741 taggagtccc tggttttcta tcaagtgata ggcaacttcc acaagtgttc acacagacca
    22801 tagtgctttt ctgtagcaat tgtaacccac aatgaccatt caggcaggtc tatat
//