LOCUS       KO-first_condition_ready_79472_MGI:1933947        22657 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Rtn1 targeting project(s): 79472
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 209522
COMMENT     backbone: R3R4_pBR_DTA+_Bsd_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     promoter        399..911
                     /note="PGK promoter"
     CDS             925..1581
                     /note="DTA"
     misc_feature    1706..1959
                     /note="BGH pA"
     promoter        2034..2100
                     /note="EM7 promoter"
     CDS             2101..2499
                     /note="BsdR"
     CDS             complement(4193..5053)
                     /note="AmpR"
     misc_feature    5272..5395
                     /note="R3 gateway site"
     misc_feature    5401..5408
                     /note="AsiSI"
     misc_feature    1..5433
                     /note="Synthetic Backbone"
     misc_feature    5434..11171
                     /note="5 arm"
     misc_feature    11199..11223
                     /note="B1 Gateway"
     misc_feature    11236..11283
                     /note="Frt"
     intron          11284..12158
                     /note="mouse En2 intron"
     misc_feature    12156..12158
                     /note="Splice Acceptor"
     exon            12159..12331
                     /note="mouse En2 exon"
     misc_feature    12343..12936
                     /note="ECMV IRES"
     gene            12937..15997
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    16036..16279
                     /note="SV40 polyadenylation site"
     misc_feature    16283..16316
                     /note="loxP"
     promoter        16343..16862
                     /note="human beta actin promoter"
     gene            16888..17682
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    17882..18123
                     /note="SV40 polyadenylation site"
     misc_feature    18140..18187
                     /note="Frt"
     misc_feature    18194..18227
                     /note="loxP"
     misc_feature    12173..12250
                     /note="FCHK std_gpr"
     misc_feature    18243..18263
                     /note="B2 Gateway"
     misc_feature    11172..18293
                     /note="Synthetic Cassette"
     exon            18655..18862
                     /db_xref="ENSEMBL:ENSMUSE00001307858"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001307858"
     misc_feature    18294..19125
                     /note="Critical Region"
     misc_feature    19149..19182
                     /note="Downstream LoxP"
     misc_feature    19126..19205
                     /note="synthetic loxP region"
     misc_feature    19206..22657
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc
      421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
      961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
     1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
     1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac
     1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt
     1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact
     1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct
     1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat
     1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga
     1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
     1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac
     2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
     2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac
     5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac
     5401 gcgatcgccg gcggataaca atttcacaca ggaatcatcc acagggttac tgaaacatct
     5461 agaaatagtg ttggcatttt tttcttgaca gctagcttgg ctgtgtttag gtggtatgaa
     5521 gaaagggtac caggagttct gccagcagat gcccagctgg gctcggggct cctggactaa
     5581 tgctttgaag tctcactgtg tgggagactc tgtcttcgta agtggaattc accatttgtt
     5641 ccactaaaaa gcatcacctc tgaacgtctg agtttaaggt gttaagagct cagccaccct
     5701 ctattacaat tctcttgttt aatgtgcaat gggggactaa ggggagcaag ttactttagt
     5761 gtctttgtgc cagcactcag tgatatattg ctggcctcgg gaacatcagt cagacaccga
     5821 ctttcccgtc atttctttgg acactggctg gggaccctgg gcttgtagag ccttctgtca
     5881 gttctcatga ggttgatggt acccatcaat atcagattat ttgttagcga ttctctgttt
     5941 gcaatactga tatttctaca acaattactt ttaacatttt cctatttggc cttatataaa
     6001 atagcattaa cttgtgattc aatggcacct atgatctttt ctggactagg gtggtagctg
     6061 gacataatag tgtatgcatt gggaggcaga ggtagaagga gaaggtatcc aagaccagcc
     6121 ttgcctacat agtgagttcg aggtcagcct tggctacaga taaagaaaaa gagaaatgga
     6181 tcaatggcgc agtcgattgt ttaatttatt gccattttat atttacttac gccacattct
     6241 gctttataaa taaaatatgg acagttgcac tactgtcttc tttgcttata catttttaat
     6301 aaactttctg aataccacag ccactattgg ggtcatataa ctgtatgcat ctaaaagatt
     6361 tccaaactga aagtatttag atcacagtaa aggacttcct ctcaagaaag ttgacagagt
     6421 ttaattttcc ataattctaa acatggaaca ctactctcat tgtgttttct ggtttggttt
     6481 ggtttggttt ggtttggttt ggtttggttt ggtttggttt ggtttttgtg agattctgga
     6541 aggtattggc acactggttt gattctgaga gtcttcagat ctcttatttc atctttgaca
     6601 gcattggttc taagtccacg acggctgtgc actcatacct gtggtgtgag gcataagatg
     6661 cgatcaccaa gccctttatc aaactgaaaa tagaacctac tgaggcctta cagatccggt
     6721 ttgttgcctc ctggctttca gcaaagtagc tgtaattatt ggaattgtca aagtgacgca
     6781 gccatgtacc tgtccttgtg agcatgctcc gtgaatgtgt gctccactgt ttagcttccc
     6841 catcgacttt ctgcacgaga atatcaaaag agggaaacta tctccacttt gctgtctcca
     6901 aggcacttga ggtcactgcg gttgtaggat tactagggaa tttaatcaag ggttgactaa
     6961 tgtggaccca ctgtgggcat ggacccactg tgggcatatt acactttgct tttcatgaca
     7021 ccgtagcatg gtcttggtaa cttgtaaaga gaagcttgtg tgggcataca attcagcagg
     7081 tccacagttg atcaagctcc tgacctagca atggcctgct tgctggcaga gccctgaggt
     7141 gctacagggt gtcaggtgac aaaagacagg gaacatactg atacatatgt gtcctggact
     7201 cctgtctcat aaagccacca gggctcagtc cacgcaggct ccccgctgag gacttagtta
     7261 gtttagtcct aatcccttct ctatgcctcc acttctaaat cccttagttg gattaagttt
     7321 ttcaccctct taaacttcac aacagggatt cagttctaaa gtgaatttcg ggggaagagg
     7381 ggatacgcta tattaaaacc atagcagata ccgtaaagct tgctaagaga ttcacaccat
     7441 tcccatcaaa attcgaatct gcaaagttga catttggaat gcagagaaca gacattctct
     7501 tctgagagca cagcttcgta gcctcccacc catcccacgc catccttgga acggggaacc
     7561 attggcctgc actgtggcca ttgccgttct ccctgtccac gcctccagct gtaaaccctg
     7621 gtcccaggta ttgccatgtt cagttctgac ccctcaaatt taagtctcct tgatgtatgc
     7681 tgtgtgacag acagttgaca agcctcaggt cagccctaaa gctactgacc atagaacccc
     7741 agatggaact ctgctgcctg catcaaccga gcccttgcct ttaatcaaag gccctcgacc
     7801 ctttgttgat ccttttattc atcccttttg gaacccttgt tgtatagcag gaccatccaa
     7861 cagaaatatc acgtcagtca catgtgatat ttaaatcttc tgctgctttt ccgaaagtct
     7921 cgcgtccctt tcctcttttc ttgggaagaa cggtgatgac tgattcatcc tgtcttcccc
     7981 gagtccttgc tcaaccacct gtcttttctt ttcagaaatc ttcacccatc cgttttctct
     8041 ccagtcccag atttttctcc accagaaggc tgagcagagc acacagttac cgctgcttgg
     8101 aaggccatgc tctctggtcc tctggatttc catttctcta ggccccgtac tgctctgccc
     8161 cacctctagc ttccagcaca gtcccctaca cagtaccgca tgacccttct agccaccaga
     8221 cccagaggct ccttcccagt cctcacttgc cttgatggat ccgcactgtt tcccatggaa
     8281 tgcccagaag cttctgtctt cacagacact gaaatctaca ctgctgtgtc tacctcttct
     8341 agcccccagg actcttgttg tagccctact tctgccatat tgcttcatcc cgttgggttc
     8401 gttagcctcc tatttcccag agattcccac ttccaggcct tcaattgttt tgttctgttc
     8461 tggatgcact catgtgccaa tagtctattc tgttcatcaa actaatcctg cataatgttc
     8521 cctttatgca cccccccaag cacgtgcccc aagtttacca agcactccca cttcttcctc
     8581 catccactcc aggactaaca acctcggact cgtagctcct cctgcaatac agacagactg
     8641 accttgtgtt agggtttgtt cagtggactg tccggtcttc ccagctagac cttcacatcc
     8701 ttaaaaatac agaaagaaca ttttgtaatt gcttgattcc tatgcttatt agaaagccag
     8761 tgatcaagaa atatgataag gacttgattt tactaaaaga aaaaaaagac aaccagttta
     8821 aatagctact ttattacata gtgagaaaac atgttaactt ttagcttctt cagtgtgcta
     8881 cttgtctaat ggagcttatt gtaaggctta aatgaaataa gacatgagag tgctgtgaat
     8941 attttagtat ctacgtggat aatattgaca caaagcactc caagcccaca gctcatcctt
     9001 tacaagactt ctggtgattg cctaagtaga aacttcttgt aatcttagat tgatcaccaa
     9061 acacgtgaac tcatttaacc tctctgccta cagatccctc caagcctgcc ccagactggg
     9121 tgccaactgt tctcagctct tttgaacagg tagccagaga tgtagaaggc tattttaggg
     9181 ggaaacctat attaacaggt ctttagcgac ttgttcagtg gggattcgag ttgaaatggc
     9241 aaatgctatc tctttccagc ctgaaagctc cttgcagggc ttcccacgcc tctttgctag
     9301 tcacagggtt gtgtgaacca ccatccccag gcctttccct gtcctccagg agaactcggg
     9361 cttccttaca aggcatctgt atcacccgcc catgatacta tgctgcctgc ccttaaagaa
     9421 attacatcca ttcctttgta gctgtaagat ctccattttc atccttcagg tattctcatg
     9481 gctctgtctc ccagcacaaa gcctggtgtt tggttctccc tcagtgaggc attagcttaa
     9541 tgaagagagc tgttggaggc tcctcacact ctcaggcctt cagcctggct ggtttagact
     9601 ccaccaaggc ctccttggcc ttattgcctc caggcctgcc aagaggagcc cagccatgtg
     9661 gagatcttgc cttttccctc ctctgtcacc tgtcctcagg gctgcaggac ttcacagagc
     9721 atttcttccc agaggtcttc tgcacctaga aacattgcac gttggcactc ttccgaaggg
     9781 aaatttcttt agttggatcg tgcaagctct gtgtgtgcct gtgttccctt ccacattgtt
     9841 aaggaaagca aatcttaaaa aaaaataaaa aaaaaaaaaa aggaaagcaa atctttccat
     9901 gcagtctttg gttttggcat cctggcattt ttttttaaaa aaatgaacac aatggtgata
     9961 tatagatttt tgaggcggaa aaaaaaacca atggtccaaa taaattaatt tctagttggt
    10021 ccaaataaat taatttctag ttatcagtgt gacaagtgac tgggtagaaa gaggctttag
    10081 cacagcaaga acttctggca tagacttggg gccctgatcc tatttttaaa agggaaataa
    10141 aaaaaatgtt atctcctccc cagacatgct tttgagcttc tcctctctct gcttcttata
    10201 tcagagaagt gtcctaagag gcagggactc acactttgtc aaaccgatag gtgtctgctg
    10261 gcaactttgt gttggagcca ttgagggacc acagtgatgg tgcaagccag ttgataggga
    10321 aggattttct ctccagtgtc gctttgccgg gggcccttga gaccggagaa aggagccttt
    10381 cgccctgcag cccctgagat gagttttctg ggaagaggct ttagctcttt gctgtacaat
    10441 gtttccatgt cacctcatta ggccttgatc gtcagcaggc atggtgtgtt gtacaggttg
    10501 atcatctcca tctctagata ctgatatgct acaaatgaca aattccacac catgaaactc
    10561 tgtagcctgc acagaaatgc tttgctgtct agaatgacct tcacactgtg tgtatgttaa
    10621 ataagaaaca taaatgggct tcgtgtttag actcgatggt tccagggcgt tactgtccat
    10681 gcctggtgtg gtgggaagat ggcaggacac gggtggtgct ggagcagaag ctgaaagctc
    10741 acatttttat ccacaagtag gaggcaaagg aggagacact ggaaaaggtg tgggcttttg
    10801 aaacctcaaa accaggcccc cagtgacaca tttcctctaa gaaggccaca cttcctaatg
    10861 ctccccaaac aattctacca actgggaacc aaggattcaa atatatgaac ctatgatggc
    10921 ctttcttatt caaaccacct cagggagcat acgaaggttt aatcccggtg gggctggcag
    10981 cacagtcctg taataataag ctaaaaatgt aaagagggct aagagatagc tcagtgatta
    11041 ggttaaatgc ctcgctcaca catgagaggt gctgggtgca atcctcagcc ttgcaaaggt
    11101 caacttcagg ccttgttaca cagccatggg tctcagcttc tcttggattt tggaagaaaa
    11161 ctaattttgt taaggcgcat aacgatacca cgatatcaac aagtttgtac aaaaaagcag
    11221 gctggcgccg gaaccgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
    11281 ttcgaaccct ttcccacacc accctccaca cttgccccaa acactgccaa ctatgtagga
    11341 ggaaggggtt gggactaaca gaagaacccg ttgtggggaa gctgttggga gggtcacttt
    11401 atgttcttgc ccaaggtcag ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg
    11461 ggtagaaggt gagagggaca ggccaccaag gtcagccccc cccccctatc ccataggagc
    11521 caggtccctc tcctggacag gaagactgaa ggggagatgc cagagactca gtgaagcctg
    11581 gggtacccta ttggagtcct tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg
    11641 ctcctcctgg gaactctact gcccttggga tccccttgta gttgtgggtt acataggaag
    11701 ggggacggga ttccccttga ctggctagcc tactcttttc ttcagtcttc tccatctcct
    11761 ctcacctgtc tctcgaccct ttccctagga tagacttgga aaaagataag gggagaaaac
    11821 aaatgcaaac gaggccagaa agattttggc tgggcattcc ttccgctagc ttttattggg
    11881 atcccctagt ttgtgatagg ccttttagct acatctgcca atccatctca ttttcacaca
    11941 cacacacacc actttccttc tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc
    12001 acccccaatg cccaacactt gtatggcctt gggcgggtca tccccccccc cacccccagt
    12061 atctgcaacc tcaagctagc ttgggtgcgt tggttgtgga taagtagcta gactccagca
    12121 accagtaacc tctgcccttt ctcctccatg acaaccaggt cccaggtccc gaaaaccaaa
    12181 gaagaagaac cctaacaaag aggacaagcg gcctcgcaca gccttcactg ctgagcagct
    12241 ccagaggctc aaggctgagt ttcagaccaa caggtacctg acagagcagc ggcgccagag
    12301 tctggcacag gagctcggta cccggaagat ctggactcta gagaattccg cccctctccc
    12361 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
    12421 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
    12481 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
    12541 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
    12601 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
    12661 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
    12721 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
    12781 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
    12841 acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
    12901 tcctttgaaa aacacgatga taagcttgcc acaaccatgg aagatcccgt cgttttacaa
    12961 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    13021 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    13081 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
    13141 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
    13201 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg
    13261 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
    13321 tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg
    13381 tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac
    13441 ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt
    13501 gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc
    13561 tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat
    13621 gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac
    13681 tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg
    13741 cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt
    13801 ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg
    13861 gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc
    13921 cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga
    13981 ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg
    14041 gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat
    14101 ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa
    14161 gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg
    14221 ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt
    14281 gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat
    14341 cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac
    14401 accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc
    14461 ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg
    14521 ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac
    14581 tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat
    14641 cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt
    14701 ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg
    14761 ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc
    14821 gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg
    14881 cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc
    14941 gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg
    15001 caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg
    15061 cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc
    15121 gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt
    15181 aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat
    15241 aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac
    15301 attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg
    15361 gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat
    15421 gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc
    15481 cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg
    15541 gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca
    15601 gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc
    15661 gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg
    15721 agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc
    15781 ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat
    15841 cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg
    15901 attggtggcg acgactcctg gagcccgtca gtatcggcgg aattccagct gagcgccggt
    15961 cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag gggggatcta
    16021 agctctagat aagtaatgat cataatcagc catatcacat ctgtagaggt tttacttgct
    16081 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
    16141 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    16201 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    16261 tcttatcatg tctggatccg gaataacttc gtatagcata cattatacga agttatgttt
    16321 aaacggcgcg ccccggaatt cgccttctgc aggagcgtac agaacccagg gccctggcac
    16381 ccgtgcagac cctggcccac cccacctggg cgctcagtgc ccaagagatg tccacaccta
    16441 ggatgtcccg cggtgggtgg ggggcccgag agacgggcag gccgggggca ggcctggcca
    16501 tgcggggccg aaccgggcac tgcccagcgt gggcgcgggg gccacggcgc gcgcccccag
    16561 cccccgggcc cagcacccca aggcggccaa cgccaaaact ctccctcctc ctcttcctca
    16621 atctcgctct cgctcttttt ttttttcgca aaaggagggg agagggggta aaaaaatgct
    16681 gcactgtgcg gcgaagccgg tgagtgagcg gcgcggggcc aatcagcgtg cgccgttccg
    16741 aaagttgcct tttatggctc gagcggccgc ggcggcgccc tataaaaccc agcggcgcga
    16801 cgcgccacca ccgccgagac cgcgtccgcc ccgcgagcac agagcctcgc ctttgccgat
    16861 cctctagagt cgagatccgc cgccaccatg attgaacaag atggattgca cgcaggttct
    16921 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
    16981 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
    17041 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
    17101 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
    17161 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
    17221 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
    17281 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
    17341 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
    17401 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
    17461 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
    17521 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
    17581 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
    17641 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
    17701 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
    17761 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
    17821 gcggggatct catgctggag ttcttcgccc accccccgga tctaagctct agataagtaa
    17881 tgatcataat cagccatatc acatctgtag aggttttact tgctttaaaa aacctcccac
    17941 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
    18001 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    18061 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    18121 tccgggggta ccgcgtcgag aagttcctat tccgaagttc ctattctcta gaaagtatag
    18181 gaacttcgtc gagataactt cgtatagcat acattatacg aagttatgtc gagatatcta
    18241 gacccagctt tcttgtacaa agtggttgat atctctatag tcgcagtagg cggctgttga
    18301 gaccaaatat tggccacgga gtatccagtt atgcttgtaa tcagtgtggt cagagggaag
    18361 aacaggccct ctctggactc gcatatgccc cgccactgtc caggtaacat atcccacaga
    18421 gatgcaccat agcgatgcgc ttgcacactg gagccatggc tgcgaatctg ggaactcagc
    18481 atcagacact cttccaaatc caaatggagt atccatcagt cagtgtgtct ggtgtcgcca
    18541 tctctctcct cacggcccgg ggtgggagac tgcagcccca gcctcagcag cggagccccc
    18601 tcagcttgcc cgggtcctgt cggagtcacg tgctgtctgt gttcccttcc acagctattg
    18661 accttctgta ctggcgggac atcaagcaga ctgggattgt gttcgggagc ttcctgctgc
    18721 tgctcttctc cctgacccag ttcagcgttg tgagcgtcgt tgcctacctg gccctggccg
    18781 ccctctctgc caccatcagc ttccgcatct acaagtccgt tctacaagct gtgcagaaaa
    18841 cagatgaggg tcaccctttc aagtgagtgg cccaagctgg ctagcccgca ccccgcctct
    18901 ctgccccacc ctcaccccgc cccatcttat tgctagctct ctgggaaact ggggcggcgt
    18961 gtttccccca caactccgcg aaccccctag acttttccag aacccctgtt tttgcttcct
    19021 aagattgctg gtaggttctg tatcaaagtc attcttatca aaaccctact ggctttcaaa
    19081 aataagcaca acccgttagc tatgtgtaat gctttaaaaa aaagagagat ggcgcaacgc
    19141 aattaatgat aacttcgtat agcatacatt atacgaagtt atggtctgag ctcgccatca
    19201 gttcaatttt actttgtttg tgtgaaatgt tttgcctcat gtatgtgcat cacgtgtctg
    19261 tcttttggac cctctgaaac tagggttata agtggttgta gccgccatgt aggcactggg
    19321 aatggagccc aggtcctctg aaagagcaac aagtgctccc aacctctgag ccactattcc
    19381 caatagtgtt ttcttttctt ttcttttctt tttctttttc acttaaagag agaatgtcaa
    19441 caactactta atccatccac ctccataagg aaggcagagc aggcactgtg accccagttt
    19501 acagatggga acactgaggt tacaagaagt aaggtcatgt ggccagtaaa taccgtagta
    19561 tgaggacggg gtgccagttt tttatttttt tggtaaagcc ccagcctacc cagatgcaac
    19621 caaccaactc cactgtagta aactcttcca tccaacttca gtggtgagca gggttgcctg
    19681 cagaagtcag tgagagtgag tgtcccacac acctgactgc cggccatagg ctccactaca
    19741 cacgtgctct atcgagtgtt tgagggacag tgctgagacc tgggtgagcc atgtagagga
    19801 tgccgaagca cggctccggg ccgtctctcg tgttcaccag caccacttct gtatgagccc
    19861 ttgctctgcc tcatccgtca gctgagttaa aacctcagtt gcaacagagc ctcagcaaag
    19921 aaaacgatgt ccatctgggg caactgggca gcctgaaagt aaaggctgct tgtcagacgt
    19981 ccctcctcag catcttggtc ctgagctcag cacccagcac aatgatgggg cgcacatccg
    20041 tggtgcatat ccgtggcaca catccgtggc acttcaccat gcactgttat ggtacgatca
    20101 tgggacctct ttctaatgcc gagtcactgg gccatctggg ctgtagcttg gtttcacatg
    20161 tccccacacc cacaggtgag agctcagagg tagctcacct ttgtttctag cagcgcatac
    20221 acaacccaga tttcagtggc agtagcatgg ggccgatgtt gaggttggag tgggagctga
    20281 gaaaactcac tttggaggca aaagcttaaa agtgaaagtt ttcttgtctg agtgctcagg
    20341 gagtcggcca gtttgggatc tgaactgcat tcctaaagag acattgtaca tcatttttaa
    20401 aggatgtgaa cacaaatcaa ggggtgagcg ggggtgagct ggagagagct gcagtgttat
    20461 gcaattgtat tttgatatta tgggtaaagc aagggagtac ccgttggaga ctgggccatg
    20521 tctagggcac ctggcttcaa ggtctttaat tcattctcct ggacattagg ccaggagctc
    20581 agagtgataa ggggacaaag gatcaggtca gctgcatgta gttaattagg gcagagcagt
    20641 cagttgggta tatattttta taacttggtt tggaaaacag caatttctat attattttta
    20701 cctgttaaca gacaattaag aaaacatttg gagaagtggg gtaggtgttc gctcctacat
    20761 gggaacatga acgtgtttga ccttgccaac aagatgaaga agctgtccct gagatgtctg
    20821 cactcagaca gctgtttaca gcagaagctg ttcagctgta gggaagtccc caggtcccct
    20881 aggacctggg accttgaatt tagttccttc ctatgattaa atggagtctg aaaatgaaat
    20941 ggtagtcctt agaatatgtt tctttcgttt gttcccaaca gcccaagtcc tcatcttcac
    21001 ccctcccctt ggccctggcc tccgtctgtc cttagtcact ttctacttca ttgtagaact
    21061 gccgtgttct catcccccaa ggcttaaaca tgggggattt aggtcccatt tgccttattt
    21121 cttcccatac ctatgtacag gaacaatagt ctaaaactac actcagcctg actattttat
    21181 tttaaactac acattgtcct ttagcaggca aatgtaaagt ttaaaattcc ttctgccaag
    21241 tcaggtgtat gagtaaaggc aatacctgaa tctgtatttt tttttaatca tatctcacta
    21301 tctggataag tcagtatttt ttgtatttct tccaagggga gaaaaacctt aatatttaaa
    21361 tataaactcc caaagtcggt ttcggttgtg tcttagtcag ggtttctatt cctgcacaaa
    21421 catcatgacc aagaagcaag ttggggagga aagggtttat ttagcctaca cttccacatt
    21481 gctgtacatc accaaaggaa gtcagggctg gcactcaagc aggtcaggaa acaggagctg
    21541 atgcaggagc catggaagga tgttacactt actggcttgc ttcccttggc ttgctcagct
    21601 tactttctta tagaacccag gaatggtccc acccacaatg aatggaccct cacaccttga
    21661 tcactaattg agaaaatgcc ttacagctgg atctcatgga ggcatttcct caagggaagc
    21721 tcccttgtga taactccagc ttgtgtcaag ttgacacaca aaaccagcca gtacaggttg
    21781 ttttaaccaa gtaccataag ctctttggat taaatgataa aaaacacaca catttctccc
    21841 atttgtgcta aatctgtaac caaagtgcct tgtggtggaa ttataaactg ctatcttttt
    21901 gttgggtcct tacaaggcat caagagttct ctggggctcc aataaagagg attaaactgg
    21961 ttctgaaggg ggccagtctc attcaaaagg gtcccgtcct cttataattg ccttcccaag
    22021 tacctggatg atcaggtcag ccacattaag agtgaggatt tcaacacatt gtccaggagg
    22081 agtccagcct ttagtccata actccccata cagctcagga caaaggctgc ttaactgtcc
    22141 aagtctgttt atcccatgcc ttagagaaat ctaggctagg caggcagccg tcttcaggta
    22201 ggagaagggg ggagaaagaa gggaagacag agctcagtgg aggggtgtgg ccatgcatgg
    22261 aaagcttatg tatattgtgg caatatgttc tgtattttat ttagtattga gttaagtctc
    22321 accacttgaa taaatgaaaa caaaaccctc attgttaaaa ccaccactca ctaagaccaa
    22381 tgagtgttta atgaggtcca ggccactgga gttaccattg ttctctggag aaatctggaa
    22441 gtcaatttca gaactaaaac agaaattctt agagtttgtc aaggaacagt ttggcagcgg
    22501 ggcttttcag atcgctgtag tatctgaaga agacactcat tcctttataa gttgtgtgtg
    22561 gtgcaaccag taaaggagct gactcttcac cagtctttct cagtccttcc ttgtttctct
    22621 gaattccaac ccacccttta atactgtgtt ttataga
//