LOCUS       KO-first_condition_ready_40813_MGI:99430        20066 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Hira targeting project(s): 40813
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_gt1
COMMENT     design_id: 44710
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     exon            5994..6056
                     /db_xref="ENSEMBL:ENSMUSE00001273463"
                     /rank="2"
                     /note="ENSMUSE00001273463"
     exon            7674..7784
                     /db_xref="ENSEMBL:ENSMUSE00001295887"
                     /rank="3"
                     /note="ENSMUSE00001295887"
     misc_feature    4112..8379
                     /note="5 arm"
     misc_feature    8407..8431
                     /note="B1 Gateway"
     misc_feature    8444..8491
                     /note="Frt"
     intron          8492..9366
                     /note="mouse En2 intron"
     misc_feature    9364..9366
                     /note="Splice Acceptor"
     exon            9367..9405
                     /note="mouse En2 exon"
     misc_feature    9406..9422
                     /note="Frame 1 insert"
     misc_feature    9423..9476
                     /note="'self-cleaving' 2A peptide"
     gene            9477..12554
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    12555..12608
                     /note="'self-cleaving' 2A peptide"
     gene            12609..13400
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    13599..13841
                     /note="SV40 polyadenylation site"
     misc_feature    13858..13905
                     /note="Frt"
     misc_feature    13912..13945
                     /note="loxP"
     misc_feature    9381..9496
                     /note="FCHK std_gt1"
     misc_feature    9381..9398
                     /note="FCHK QC prefix region"
     misc_feature    9399..9478
                     /note="FCHK QC critical region"
     misc_feature    9479..9496
                     /note="FCHK QC suffix region"
     misc_feature    13961..13981
                     /note="B2 Gateway"
     misc_feature    8380..14011
                     /note="Synthetic Cassette"
     exon            14424..14514
                     /db_xref="ENSEMBL:ENSMUSE00001221961"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001221961"
     misc_feature    14012..14794
                     /note="Critical Region"
     misc_feature    14818..14851
                     /note="Downstream LoxP"
     misc_feature    14795..14874
                     /note="synthetic loxP region"
     exon            16146..16240
                     /db_xref="ENSEMBL:ENSMUSE00001267295"
                     /rank="5"
                     /note="ENSMUSE00001267295"
     misc_feature    14875..20066
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg aaatgacagg gtaagtgtta gctattgtta
     4141 atttgaatac aggagaccat agaacacgta gtgattgcta aactttccaa aggtttctat
     4201 ctgtatttgg cacacctgtt tcatgtttct cctactacct gccacccagt gaccagtaag
     4261 aatgaaaaga tatttcactg ttatgcttga tttgatcaca cagataagac tctggaagat
     4321 gatgccaggg cgcatcacca aggacccata ttgattactg cctttgggta cctgcactgg
     4381 attttccaca tggtgtgggg tccctggcag ggtttacagc ttccctcagg tagacaccag
     4441 gagctctaat aagtgataag taatcacctt atcacttgtg aaattctaga gcttatgacg
     4501 ttttgtccct gtctgcaaca gtagttgctg atgtttagtt gactgtcata cttctaacct
     4561 gtcactcaca ggctgatgat atagtttaaa ttcagtagca gttaatatga aacctgcagc
     4621 ttttgttgct ttggtcttgg aaacagacca gggacatgaa cagataccaa ctaggcccag
     4681 ggtccttaga ttaccttgca caaactactc ggtgttggtt tcattgcctg acttggtctg
     4741 tcctgcaagc caattgcctc agccaagcct ggatgcagag gggcccttgg ttgtttctaa
     4801 gagcctgctt tagctgttta cccttaggaa ctgattattg aatgtagttg agtatatgct
     4861 taaacagagg tccaggcagg ggatgctgag agagtagact ttctatacta ctccagggga
     4921 gatgtgaacc aatggccaac caaaggatat catcaaagtc caacctggtg agcccttttt
     4981 tttttttttt tttttgatta cttgcaggag tatgcatgag gggttacata caggagtatg
     5041 taactcaaag acagctgcat ctccgaaagc ccaccctagc atgggtgaca gctcctgaaa
     5101 gctggaactt gaagtgtacc acacagcctg catgcagctt gatagactag agagtatctc
     5161 ttccagcatg ctcagttggt ctagcctttt ctaggcaact cagctgctct gaaagtctct
     5221 cagtaggctt tactgcttag acactcttgg agaggaaggg aacgagtatg tcctgtgact
     5281 aagtaaggtt aagtcggttt caggtttctt gaggtttttg ttgtttactt tctagtttta
     5341 aggagcttca gtgagtatgg aatgttttac ctcccttcag aacattatga gtgttccaag
     5401 cttccctcca agattgagtg ctttatcttg gaattgctac acaccatgtg tgtaactata
     5461 gactttcagg accgttgtca ggaactaaaa tcttaacaag agaagggagg gcccttggct
     5521 ataatacatc attagacctg cttatgacag ccagccagtc agaaaaggat ggagagtcct
     5581 tcttcccaag ctgtcagcca agtgagactc acactgaggg agatgtcatc aggtagagat
     5641 atggtccttt cacaggagct tgtgacttga gcttttatta cagtttattg cctttatata
     5701 ttcaggtccc caggaagtac ccagcaatgc tgtagtctct tgctttcagt gatgacatac
     5761 ttagtctctg ttgggttttc agctatttaa tgttgcttgg agagtctaac agttttgact
     5821 aactaaagct gttagatctc cccattctta caggtaattt catctgcagc ctgctgcttt
     5881 gtccttgatg taaatttaga gatgttattt gagtaatcaa tattttaaaa ataaaagttt
     5941 attgttttct agtgaataaa agccaagctt gatatgtttt tctttgtttc cagggaagcc
     6001 aattttttca gttgatattc accctgatgg gaccaagttt gcaactggag gacaaggtat
     6061 gtttgtagag tgaataccac ttccctgttg ccgtactctg cttcttttgg atccatggtt
     6121 agctgtaatg agacaacttg ttgctttgta atatgactgt gactcagttt aaagtgcaca
     6181 gtttgaacag gttagttaaa tcacaacctt tttttttttg agctggatat ggttgcatag
     6241 gcctgtgatc tcagcacttg gaagacaggt agatagaaat tctaggtagc ctagattaca
     6301 aagtgaagtc cgacttgaag atgaacattt tttggggtct gtagggtgat cttagaaata
     6361 ttgtgcagct gacttatcat tctaattaaa gctttttatt aagctaaagc tagaatgaaa
     6421 ttggtgcacc cctggtatta gatataaatc agcaggaggg tgtcctcccc ccccccaaag
     6481 tccagttaca gagaggcaag gattctgtca gtgttttcat ttgaatgcat gactacactg
     6541 tgatttttct ggacaaagga atttgactgt gttaatgaga aaataagaat gtctagagca
     6601 tttgaaaata gcatccatag tatcagtgtg ttccattcag catctactgt ggtattgaga
     6661 taacatagct ctgtttggaa agacgtaaaa caacttcctt tttttttttt aaatgccaga
     6721 aaacaaagtt tttatttata gtgataagaa gtagcatttg ctcaaggcca cgcccccacc
     6781 ttccaggtgc tacagggggc ggggctcaca gtaggcaatt tgcacagagg caggagtgaa
     6841 ggcagttgtg gttgaaaaag tttttccccc tggtctaaga gggataatta ggaaatattt
     6901 ttacatatcg gctgaagaag aggtttaaat cattttgtca ttactttgga acatccattt
     6961 cagacattca tttttttttc atgacttgtg ttaactatag tgatggaaag caagcaaaca
     7021 aacaaaaata attagtataa tgtatagata gacttttcag tacagctggc atcaatttat
     7081 gaccctccta aattccctct tttggttgat ctccatctat aggtgactgc aaagggcaga
     7141 taagaaggta atgagccctt tttcctgtgt tcagggcaca tttcaaattt gggtctcaaa
     7201 gcgtccagtc aattctggaa acagccatcg gatccaggca gcaccttgaa ggggagggaa
     7261 accctggtag ggactgcact tttctccttt tcctcagtgc acatgcagac ctgaaggggc
     7321 ctgacagtga ctttccttcc tctttgtttt gtagatgagg agtctgtgtg ttctggatag
     7381 gaatgaggac ctcagaggcc agacaggctc tctctcttcc taatttgtag ttcagtcagt
     7441 attcttttta taaagacagg ctggcctcag ctactattaa attcactctc ttttgggtcc
     7501 ctgttatttt tttccctcac cttttgtgtc atttacatta gtaattcaag ataagtctgc
     7561 ttagatctag tcaagtatga tttaagaaac taaatgagca ttttctatag ttactgttat
     7621 cattgtcgtt tccacttctt gcatattgag cttttcttgc tccttcattc cagggcagga
     7681 ttctgggaag gttgtgatct ggaatatgtc tccagtcctc caggaggatg acgagaagga
     7741 tgaaaatatt cccaagatgc tttgccagat ggacaatcac ttaggtatta cagagtggcc
     7801 ctttgcaggc tttgcgtgtt ggcagagtgc ccaaggttag cacatgtgtt tgtaattaga
     7861 aagataaggc cccgtgcggc gttaactccc ctcggcctgc tgtctgaagg cacagatgtt
     7921 ggctgcacac ctgggtgagt tattcggcag aagactcggc ccgcccacag aaccatcttt
     7981 ggacagtctg caggtacctc tggtgatttc ctgctcatga gtcgttactg cctgattcca
     8041 tggggctttg ggaccagtga cctggacaca gtagggttct ggggaagggt acataagaga
     8101 cagctcaaca catgtctcag attggtgctg tgtcagtaag agtctctctg ccaggacctt
     8161 ttagaatgtg gaacaatttc atgttttcct tccctgttgt atgtaatgcc ttagtgacca
     8221 ttgacagtta ctagtgtttg gtattagttc cttcctctgc tttgtttgtt cttccaagcc
     8281 tctcagtgtc actgggtcta gagcataagt tgctcatggg ccatgtatca acttcaacat
     8341 tgttagggtc catcatattt catatctcca aagactgaga aggcgcataa cgataccacg
     8401 atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc ctattccgaa
     8461 gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac cctccacact
     8521 tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga agaacccgtt
     8581 gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt gggtggcctg
     8641 cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg ccaccaaggt
     8701 cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga agactgaagg
     8761 ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc aaggaaacaa
     8821 acttggcctc accaggcctc agccttggct cctcctggga actctactgc ccttgggatc
     8881 cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact ggctagccta
     8941 ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt ccctaggata
     9001 gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag attttggctg
     9061 ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc ttttagctac
     9121 atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg gtcagtgggc
     9181 acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt atggccttgg
     9241 gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt gggtgcgttg
     9301 gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct cctccatgac
     9361 aaccaggtcc caggtcccga aaaccaaaga agaagaacgc agatcgcaga tcgcagatct
     9421 ccgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctggga
     9481 tctggactct agaggatccc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     9541 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     9601 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct
     9661 ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg
     9721 atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca
     9781 acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt
     9841 gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta
     9901 tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg
     9961 gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa
    10021 accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata
    10081 tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa
    10141 tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg
    10201 ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc
    10261 agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc
    10321 gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga
    10381 gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc
    10441 tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc
    10501 tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc
    10561 tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga
    10621 acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt
    10681 gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc
    10741 caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc
    10801 gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat
    10861 caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc
    10921 gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga
    10981 tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa
    11041 aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga
    11101 tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt
    11161 tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg
    11221 gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct
    11281 gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac
    11341 accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat
    11401 acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc
    11461 cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac
    11521 tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc
    11581 aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc
    11641 tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca
    11701 ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt
    11761 caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg
    11821 atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg
    11881 accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt
    11941 tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt
    12001 ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg
    12061 gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga
    12121 ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc
    12181 cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat
    12241 tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc
    12301 gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct
    12361 acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca
    12421 catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt
    12481 cagtatcggc ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc
    12541 aggggatccc ccgggagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
    12601 ccggccctat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
    12661 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
    12721 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
    12781 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
    12841 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
    12901 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
    12961 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
    13021 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
    13081 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
    13141 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
    13201 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
    13261 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
    13321 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
    13381 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
    13441 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    13501 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    13561 cttcgcccac cccccggatc taagctctag ataagtaatg atcataatca gccatatcac
    13621 atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    13681 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    13741 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    13801 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgggggtacc gcgtcgagaa
    13861 gttcctattc cgaagttcct attctctaga aagtatagga acttcgtcga gataacttcg
    13921 tatagcatac attatacgaa gttatgtcga gatatctaga cccagctttc ttgtacaaag
    13981 tggttgatat ctctatagtc gcagtaggcg gatggctctg gttaattgag gttatttaga
    14041 gctatgtgtt atttagaact tcttggaaag ttctgggaaa gacatcctgt tttattttga
    14101 gaataattcc tattgaaatt catctcctcc atagacaacc ttttggaact aatgaagttt
    14161 attgctgttc ataggaataa acttcctgtt gtctcattca gaacaattct cagattaggc
    14221 atattttccc tttagactat tctggttatt tgttatagca caagattagt gtttaactga
    14281 attgtttctc tgcgactcat acacagtaaa tgctttaaat agattaggta gttttataaa
    14341 ccttttactt cttgccagat tctacataat gcaaatgggc atgcctattg gattttcatc
    14401 accttatttt tttttcttcc cagcatgtgt gaactgtgtg cggtggtcaa acagtgggat
    14461 gtatttagct tctgggggag atgacaaact gattatggtg tggaagcggg ctacgtaagc
    14521 atcattttcc tttttcacat ttaattttta gaagcttctt tgctggtctt tatcaaagtc
    14581 acctttccca ccctggcata gaagtcctaa cttcataagt agcaagaagt tgccataggt
    14641 cagaagtgga tgaagcctga caagtgactc ggaggcttcc attctgcctg ctgaccaggc
    14701 cagctatcct cttgtaggaa cacagtactt agctgagaag gaagggttaa tccttacagc
    14761 cactgacatt gtggcttcct ttgtttgtta ttcagagatg gcgcaacgca attaatgata
    14821 acttcgtata gcatacatta tacgaagtta tggtctgagc tcgccatcag ttcatacata
    14881 caaaatgact aagatctgaa tcagggctgg gaatccggag cccaccttcc ttgcctcatt
    14941 ttgtggcagc cttgccttgc agtaaactaa gggtcaactg tggacatttt tttctttatc
    15001 cttgagcgta atgggtcctg atactgtgta ccataataag ctttccatgt ggctctcggt
    15061 cacactgtgt cccagacctg gcatcattgt ttagccagag taactggagg catagctttg
    15121 agagctgagt gatgccatct tctgtgctca cttggctgcc tcaggagctc tctgggtaag
    15181 tgtatagctt tacaagagca aagggtgttt gtagaggctc tggacatggt gccatcttcc
    15241 atgtaattct acctggaagg agtcagtcct gtgactcttg tatttttctt ccttattttt
    15301 taaagcagga atattgaatc cattataaag gggcttaaat tattgttttt tttttttttt
    15361 ttttgagtta ggcatagtgg ctcctacctg tggacccagc tactcaggag ggactggctt
    15421 gaacccagga gtttgagatt attctgggca atatagtgag tgagaccctg ccttcaaata
    15481 aaaatgtagt taatattctt cttcttcttc tttttttaat taaaaaaaga caggggtctc
    15541 atgtagccca gacccttgga tgaccttgaa cttctgatcc gtctgtccct acatcctgag
    15601 tgctgagatt accagtgtgc atcacatgcc tgggtgctta tatttactta tttatttatt
    15661 tatttattta tttatgatac cctactttaa ataaccaaca caaatactat gtttagtatg
    15721 cactttagaa atgtaaaaat agctcatagt cctacaacct aaagaaagcc atggttaagc
    15781 ttttgttttt tctggtgtgt atttctttgc tctattccat gtgtagagtg tctgtgctac
    15841 atgtagtttt taagtcatgt aaatggtatc atactgtatg tagatggtgg cccgtggtct
    15901 gcttttctct cactttgtct tgaaaatttt cctgttgaag atttgtagtg gctgaggatt
    15961 atttcacaag tgcatggatt gatcctaagt taccacttct gccacatctt ttgtttaact
    16021 ggtgctttta gctgctatct ggttttgcct tttctgtttg ctatgataag ctgtgccaca
    16081 gggagcacct ttaaacagta tctagaacac tccttgagtg catatctcat gcctctttca
    16141 cacaggtaca ttgggcccag cactgtgttt ggttccagtg gtaagcttgc caatgtggag
    16201 caatggcggt gtgtctccat cctccggagt cactcaggcg gtaagtaggc tcctttgttt
    16261 gggtgagtgg atcccttttt tttttttttt ttttcgagac agggtttctc tgtgtagccc
    16321 tggctgtcct ggaactcact ttgtagacca ggctggcctc gaattcagaa atccgcctgc
    16381 ctctgcctcc tgagtgctgg gattaaaggc gtgcaccacc gcgcccggcg tggatccctt
    16441 ttgattgcct tagataacaa ttaacacccc tatgtactgt ttggtcctta aagtactacc
    16501 tgggatttcc ctatcatcgc ttgtccttgc ttaaggttta tgtagtcact ggctccttca
    16561 cctgccttca cttgcattca attaccaaca catactgtta aaacatgtaa atgtatgtgt
    16621 ttagcatgga gctcccagcc tttcattgct tattctgcag gagtactttc attcttagat
    16681 cataaacaca ttcgatctac tcctgagata caagcctgta ggtacttact agaaaatttc
    16741 tttctttctt ttttttcttt ttcattcatt cattcttttt acatacatac atatatacca
    16801 tacataaatt aatttgtgtg tatgagcatg tctactacat gaacatgtag aggtgagatg
    16861 acacttgcct gagtcagttc ccttgctcta ccatgtgggt tctgaggatc aaagttagtt
    16921 tgccaggctt agtagcaagc cactgagcca tctcaccggt cctattggaa actctcttaa
    16981 cttgaccttg actatgttta taaagtggtt caaataggct tagtttgcaa agagtctcat
    17041 tttacagatt taagccagga ggaagatgac tggcctgtat aaagccgatg cccttggttt
    17101 ctaataaagc ccaagtactt tagttaccaa ataccatgat ctacatgtat acagttcaga
    17161 ctttggagaa ggctgtgatg ctgcaaggaa gaaactcctt aaaaaaacaa aacaaaataa
    17221 aacaagaata tacaaatatc cgttaggatt gagcctggaa ctctaggaga atacaaggtc
    17281 atagggatag atttcaattc actagtagta tatactcaac agattctttt aactttaagt
    17341 gaataaacta atttcttaaa aatatttttg gagattatat tgttgtgtga gtgaatgtct
    17401 ttgggtatat atctgtgtac tacatgtatg tggtgcctgt ggaggtcaga agaggaatgt
    17461 tggatctttt tgaactggtg tctaaggtga ttgctagcca caatataggt gctggaaatt
    17521 gaactggagt tctttggaat tgcagcctgt gtttttaact gctgacccat ctcttcagcc
    17581 tgccttgccc cctctctaaa agatacttca ttataaatta tgtgttcagg ctgggaaggt
    17641 ggtacatgct tttaatctta gtactcatga agtagaggca ggcagatctc tcagccagct
    17701 tggtccacat aatgaattcc aggccagcca aggctaaaca gtgagaccct gtaccccaaa
    17761 acaaaagaaa aacaaacaag caacacctaa tcaaccaatc aaataaacaa acaaacaaac
    17821 aaacagcaac aaaaaagccc aaaataaaat aaaataaaat aaaataatgt gtccatgtgg
    17881 atatgtttat gtgagtgcag atgcccttgg aagtgtcaga agagggcatc agatcctctg
    17941 tatctggact tataggcagt tgtgagctgc ccagtgtgat gcaatgaact gaatttgggt
    18001 tctgtataag agctgtctga agctgggcat ggtggcgcat gcctttaatc ccagcactcg
    18061 gaaggcagag gcaggcggat ttctgagttc gaggccagcc tggtccacag agttccagca
    18121 ccgccagggc tatacagaga aaccctgtct caaaaaccat aaaccaacca actaaccaaa
    18181 caacaacaaa aaagagctgt ctggatatgc tcctaactat tgagtcatct catcggcctc
    18241 taaaaggaga aacgaatctt tttgttttat cttgtcttgt tttcttctga gacaagggtc
    18301 tccctatgta gcctggctgt cctggaactt attatatagt ctgaggttgg cctcagactc
    18361 agacctctat tggcttctgc ttcctgtgtt ctgggattaa aggtgtatat caccatgtca
    18421 gctaaattcc tctcttaaga accaaagtta gaaccttagt gaaaacttca ttgagaaagc
    18481 taaagcagga gctttgtgtt gatgtttgat aacaacaagc atgtataatt tgcccttttc
    18541 actatctttc ttttttttcc cttttgtttt tttgagacag ggtttctctg tatagccctg
    18601 gctgtcctgg aactcacttt gtagaccagg ctggcctcga actcagaaat ccgcctgcct
    18661 ctgccttccg agtgctggga ttaaaggcgt ggcttccctt ctcactttca tagcaactta
    18721 cagggagtct agttgggcct cactggagct tgccacaagt atctaagagg gtatttaaga
    18781 ttaggtaggg tgtcaagtga ggagtgttat gggaccctaa cctgagactt gggctgaact
    18841 ataatgtgag atctctcctg tgccaccttc tctgagagtc acttcccagg taagttggtt
    18901 ggctgctttc tagtggctga tttcatgctt cggttcactg gttctggtag caggtctgtg
    18961 tagaactgtc catgaagaaa tgtgaggagc agatagatga gtaagaagcc actgggtctt
    19021 tgaaaagcaa aagattaaaa aaaaaaaatc tgacagattt aactagaggt gaaagaaaaa
    19081 aaaaaggaca gaataggaag tgatgatcta gaaaaatatt taagctttaa gcattaagct
    19141 aaaaacaaaa tcaaaagcaa gaaaccaact ccaagttctt aattgatcct aaagggaaac
    19201 attagaatct ttgagtggaa aaagttggaa accagggagc ttcaaaatgg gtaccagttc
    19261 tgattccctt cagtaaattc tgatctacgg cctcagacac attccctcat gttgtaatcc
    19321 ctagcctttt tacttttgat tttgagatag ggtctcatag tggttcccac actgggcgtg
    19381 ggtacattaa attcagatca gccttgaaca ttatgtcttc ttgccttagt ggatttttag
    19441 actactcagt agtctcagct ctaaattatt tattgatata ttatttcaag ttttgagaaa
    19501 cattctacag aacataaaaa tatttattct tcaaagcaag atagaaccct tggtttccaa
    19561 actccaatag aaaagtaaag gaaacaaaca cctgaataaa aaaaagacaa aaaacaaaaa
    19621 caaataaaca aacaaacaaa aaaaccagcc aggcatggtg gcacacacct ttaatcccag
    19681 cactcgggag gcagaggcag gcggatttct gagttccagg ccagcctggt ctacagagtg
    19741 agttccagaa cagccagagc tacacagaga aaccctgtct tgaaaaacaa acaaacaaaa
    19801 aaaaccaaaa aacaaacaga caaaaaacaa acaaacaaaa aaaaccaaag gaaacattaa
    19861 tagcaatgct agagaataaa atatttaaaa agttaactta gaataattaa atttgaatca
    19921 tgcagaagag attgaagtat attataatcc tactagtaga tttaaattac gtatgcttca
    19981 tattcatctt aggtattcta acaagaatac tagctaatta gtcattattc tctcataatt
    20041 atacctttaa aaagatgctc acagag
//