LOCUS       KO-first_condition_ready_78967_MGI:97772        23471 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Prox1 targeting project(s): 78967
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 240482
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10022
                     /note="5 arm"
     misc_feature    10050..10074
                     /note="B1 Gateway"
     misc_feature    10087..10134
                     /note="Frt"
     intron          10135..11009
                     /note="mouse En2 intron"
     misc_feature    11007..11009
                     /note="Splice Acceptor"
     exon            11010..11182
                     /note="mouse En2 exon"
     misc_feature    11194..11787
                     /note="ECMV IRES"
     gene            11788..14848
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14887..15130
                     /note="SV40 polyadenylation site"
     misc_feature    15134..15167
                     /note="loxP"
     promoter        15194..15713
                     /note="human beta actin promoter"
     gene            15739..16533
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16733..16974
                     /note="SV40 polyadenylation site"
     misc_feature    16991..17038
                     /note="Frt"
     misc_feature    17045..17078
                     /note="loxP"
     misc_feature    11024..11101
                     /note="FCHK std_gpr"
     misc_feature    17094..17114
                     /note="B2 Gateway"
     misc_feature    10023..17144
                     /note="Synthetic Cassette"
     exon            17654..19445
                     /db_xref="ENSEMBL:ENSMUSE00000160908"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000160908"
     misc_feature    17145..19561
                     /note="Critical Region"
     misc_feature    19585..19618
                     /note="Downstream LoxP"
     misc_feature    19562..19641
                     /note="synthetic loxP region"
     misc_feature    19642..23471
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaaaaaaa aaatcatttc ctctcctaaa ccagtcaggc aaaagtctgt
     4321 taacagaacg cacatggaca gggctggaca ggtgtgttgt gtcaagaaag acaggtgcaa
     4381 gtttcctggg gtctgtcttt ctgtacagct ccagactcca ggcttctggg acgaaggggt
     4441 ttgtgaattt atggtggttc tccttaacgg gattgtcagg ctaatgggaa tcaggctctt
     4501 ggctgttgtg tctgagcccc tgccgtgaga gggttttttt tggggggggg tagtttgtat
     4561 ttgtgtattt gtcttaagga tgttggcagg tgactgctga ggtcatatct caggcttctg
     4621 tctgccagcc tgccaaaagc aaccccaccc ccaaccccca cttcagggca gcaggtggaa
     4681 ggagttgact ttctctttgc ttccctccag tttatgcctc tctggcaagt cggtgcttag
     4741 aaggggggtt ggggggaatc tcgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     4801 gtgtgtgtgt gtgtgtgtgt gtgtgttgca cagcaatgag tagtttgtaa ctgcctaaaa
     4861 taccaaagcc agttcacaac tgtgaaggct attaaagctt gtctttgtag tgtttttttt
     4921 ctctccctgc cccaaaagaa taagtgtgtg cctaaactct ttcaaaccct gtgttaaata
     4981 ctctcataaa gtgaaccgtg cctgccattg tatcagacat gtgccaggtg tcctagatag
     5041 gactttgatg gagagggaga atctaagtct gtgagaggga gctgggcttt tgctatttga
     5101 tggaagatct gggcagccag ttctatgaag agtttcttgc aagaacttct gagtgttttc
     5161 agaatgaaac aggctgcttg cagtgagaaa gaagaaagtc tgcttttgtg caggagagat
     5221 gttgggattt ctatgtaagt ctgtctaggt tcttaaaact taaccttctg aggccgtgtc
     5281 tcctgtttga gttgtatggg aataggccag atgataaaca ttcctgttgg ttagttttgt
     5341 tatttggggt tatttgtctt tgtttccatg aaaatgaaag atacatgtca cttagtcttt
     5401 taacttaaac attagtcacc cccttctata actttctttg tatgcttttg aagattttaa
     5461 taattaaaat gttacctatg actatcttca tataccttat ttttaatagg cattagccat
     5521 gcttggcact tactgatatt ttatgtgcct gttctggaga aatctactca ttttagtcat
     5581 taccttgtga ggacaaaacc ttgcatatgc agcatgtatc attgttttcc atgtgttcat
     5641 ggctagcttg gttcctttgc aagcaggttg tgcaacctcc aagcctaaaa ctgactcact
     5701 ttgtaagtta ccagaccctt cttatttact caactcttaa catctctctc cacagatcgc
     5761 ttgtatcatt tcaagtatgg tttaggctac atactatcag tgctatcaat ataagttttg
     5821 ctacttagaa ttttaaagga aattatcaaa tgttgtcaat gttatactat gaaaacagta
     5881 cgcatggtga tgtgttgtta tatatttaaa atgcagtgtt tgtataaata catacaaaga
     5941 atttcttcag tatgattgcc tagttcaccc caagcttcaa aagacaattc tgatcatttt
     6001 ggtcttcctg ccaaaagaaa aaaagtgtga gtgtgttttt ttaagattat ttctcacact
     6061 cttttatttg cccaatttga tgaattacaa ctttgaaatg agtggaaatc aatatgttgg
     6121 gcagatctca aaaaatattc tctgttcatt tttcaagtta aaaatgaatg gaattttaga
     6181 taagatgagg acgaccaagt gaaatggtta tggtttaaaa acctcttctt gtaaaaggaa
     6241 atttaatttt ttgccaataa gatcacaact tgttaaagta ttaacttgtg ccaagtaggt
     6301 tcttcaaaaa tgtcagaggc tttcttcacc ttgtaacatg cagaaatatc tctaagtcag
     6361 aagttgctac ttagcagaaa cgcaaggaag ggacagctag tgactctgta tgtctgcatg
     6421 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgcaggtgtg tctgcatgta tgtgtgtgtg
     6481 tgtgtgcagg tgtgtctgca tgtgtgtgtg tgtgtgtgtg tgtgtgtgca catgtacaaa
     6541 tcaaagcttc tgatttcccc ctgttgaatt caggctggtt ttcaaaatga cttaaactat
     6601 tgttgatgct gtcactcaaa actacatgac tagcttaaat cattagaacc ctcccccctt
     6661 aaaaagaaat aaaatcaagt tatttcttca aataatttta tcaacctgga cagatagctg
     6721 agtaactctg gtaggaagtc atgccagcct tacactcccc atgcctcata aaagatttct
     6781 ctgaactatc tggataatta ccataatgaa acacttcttt gtctagaatc tgggctccag
     6841 atagatgtag taaaagttac acaccccctc cccaaaataa gttctttatt aaagttgagc
     6901 cctgcaccac tttacttcgt attgctgttt ctttgaaaaa ttctttactg aaaaactgtt
     6961 ttgtttggct ttttgttttt ttctttttgt ttttgttatt taacaaaaac atgaagtcaa
     7021 aagtaccctc tgcatggatc ttctttgtga gacagggtgt gggtttcagg aggactcttt
     7081 tggagcagtt aaatctgaag tgctcacgtc ttgagttgct ctcctgctcc ccctccccag
     7141 cagtggcatc tgctgcttca tgctgtctat tgtacaaagg tttcagagcg tgtgtgtagg
     7201 ctgcggacca atccgtgcaa cacatgctca ataatttagc attttaacat aatttccacc
     7261 tgaattcagg agggctttat aagacacatg tatttgggca gtgatccttt ggtccagagt
     7321 ctattatcac tatttttcca tcttctttat aatgtttctc agcaagactg gcccaatggc
     7381 tttctattgc ctgcttaaac cccagatcaa tgttaaagtg aaaaacatta taagaatcca
     7441 tagtaattgg agttgacaaa ccacagctta gttcacatgc taagagatgt tgcgttgttg
     7501 ggtggcttag tgggttgaca ctgtgggtga ctaagtcctt atgtagcatc ctggaaacta
     7561 tgggttggaa attatgggaa cccctggaaa atgtgctcac ctgtcttcct tttaccatgg
     7621 ccacttaaga gcacatttaa tgtgggggaa aaaacatttt tatatattct gtttaatgaa
     7681 atacgatatg atactaggtc agccagctat tattatttaa tcttgcctat aatttcccta
     7741 gattatggcc ctgatgtgcc tggtcactct ccttttattt ctaagtttaa atcataaagt
     7801 gatttggaga aataaattaa tgtatatcta tcaaaataat gccctgtctt gctggataaa
     7861 ataatttttc ccacttattt tgttctttat atttgttgtt tatatctatc tcagatggat
     7921 attttacagg aagacctcaa gtattggtag tcttcttgcc caaaatagaa ctaaatcact
     7981 atttaatttt taaatgatat ctgattaaaa ttttttaaaa aatcaattta gttgggtgaa
     8041 tttggaggga actacagcta ttggttctca atggatccag cctgtatgct taattattat
     8101 ttattctcca gtgaagataa agagtgggat catcctacct taggaagaac ctggtgtctc
     8161 cttttggctc tggtttaagg caggggtgca gctgttgata cctaggcaac atgagaggaa
     8221 gtgcaatgga tgtgaaggtg aatggcagct gggggaggaa ggatgcctgg gatgaccttg
     8281 tgctcatgaa cacactggca catcctctgg gagcctctgc tccagaggtg ttctccagag
     8341 tccaagacgg gcaggagaga ggaaccatct gtgtggcaag gtgaacaggg caagatacag
     8401 aaaggagttt cttccagcct tcactggagg tctgtgtgct ttacagggga tattgaaggg
     8461 ggaaaaatag atttacacat gcatacaaat gacgtaaaga tgttgaaatg atggcaagaa
     8521 gtcctggttt cgaaagctgc tgtatgcaca aaagaatctt cctgggaaat tgtccccttg
     8581 ctcccgtcct taccctgacc tgacagctgc tcctgcatta cctatcagac ccaatgtgct
     8641 gccagaaaca tatgtgttgc tgtattttgt cacctccaaa ccagttgtat tattagtggg
     8701 agaggagtct cggttgttct gaacttaata ttgaacttaa agacactgag atcgctcact
     8761 ccaacccaag ttcaggatat tttgtcgtga actgtttcgc cctcagacat ataaagatct
     8821 ctagaaagaa ataacatctt gccttcaaga tatgccaaaa ttggctcttt taaaaaacat
     8881 tttgtcatca gaaaataaca aacaaacaaa cgaaaaaccc gccaagagca aacacagcat
     8941 ggtgaaatat ttatcatgaa aaaaaaaaca acaacaaaaa tcaagaaaca aaaccaaaaa
     9001 acaccaacat attattgcca tgtaagcatg ggatattttc ttgatttttg caggttattt
     9061 catgctgaaa ttatgcctat ttgcatgggt agtcattctt tagaactatc cacagatgca
     9121 ctcctaggga gcatggagat gtttttacag gtgaaccgaa agagatggag agaatccagc
     9181 actccacacg ctgcctttgg caatggaccc tgttattgtg aagctgtgct ctgttaagca
     9241 aacgcgaagt tcaatatcag ataaacccgg catgaaggca gtattttcat tttcttcata
     9301 aaacgttaat tgtaaacaaa aagatgtgct gtctcttgca tgcatataaa atttggggtt
     9361 agcattgcta gttactaagt gacacaatgt atcaagggcc tttgataaaa tgtaggcaag
     9421 ggtgtttcct ttttatatct tgtggttccc ccgcccccag acaatgctag tttgcataca
     9481 aagaagctac ctacctttct catatatttg attgatattt tgtttctttt ctatctttct
     9541 ccagttgctt cttctgttta gtgcccccta ccccttaact tggcataggc aataagaacc
     9601 aggaatggtg tgtgatgaag agtttcttag ccatttgaag tcctgttttg acagatttac
     9661 cagaacaaaa ctagtattgt atatatgtgt gttcttgcct ggttcaataa tggactgttg
     9721 cacatactgt gggttaagta ctgtgactca taaaaacatg gcaacagtcc atctcccttt
     9781 ctaacaattg aagtcaccaa atggataatg tgaccctttc tgcacatctc tgtgttagat
     9841 gtacactcag ccatagatac catctccacc tgtgaattca ctgatcaaaa cccataacta
     9901 ctaaataagc agtattggac agtgcatgtc tgtgcctcca tctcagtact tagcatacca
     9961 cttatgctga agatgttggt tgctacattc ttcccaggtt acatgtgtca aagttgcttt
    10021 taaaggcgca taacgatacc acgatatcaa caagtttgta caaaaaagca ggctggcgcc
    10081 ggaaccgaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgaaccc
    10141 tttcccacac caccctccac acttgcccca aacactgcca actatgtagg aggaaggggt
    10201 tgggactaac agaagaaccc gttgtgggga agctgttggg agggtcactt tatgttcttg
    10261 cccaaggtca gttgggtggc ctgcttctga tgaggtggtc ccaaggtctg gggtagaagg
    10321 tgagagggac aggccaccaa ggtcagcccc ccccccctat cccataggag ccaggtccct
    10381 ctcctggaca ggaagactga aggggagatg ccagagactc agtgaagcct ggggtaccct
    10441 attggagtcc ttcaaggaaa caaacttggc ctcaccaggc ctcagccttg gctcctcctg
    10501 ggaactctac tgcccttggg atccccttgt agttgtgggt tacataggaa gggggacggg
    10561 attccccttg actggctagc ctactctttt cttcagtctt ctccatctcc tctcacctgt
    10621 ctctcgaccc tttccctagg atagacttgg aaaaagataa ggggagaaaa caaatgcaaa
    10681 cgaggccaga aagattttgg ctgggcattc cttccgctag cttttattgg gatcccctag
    10741 tttgtgatag gccttttagc tacatctgcc aatccatctc attttcacac acacacacac
    10801 cactttcctt ctggtcagtg ggcacatgtc cagcctcaag tttatatcac cacccccaat
    10861 gcccaacact tgtatggcct tgggcgggtc atcccccccc ccacccccag tatctgcaac
    10921 ctcaagctag cttgggtgcg ttggttgtgg ataagtagct agactccagc aaccagtaac
    10981 ctctgccctt tctcctccat gacaaccagg tcccaggtcc cgaaaaccaa agaagaagaa
    11041 ccctaacaaa gaggacaagc ggcctcgcac agccttcact gctgagcagc tccagaggct
    11101 caaggctgag tttcagacca acaggtacct gacagagcag cggcgccaga gtctggcaca
    11161 ggagctcggt acccggaaga tctggactct agagaattcc gcccctctcc ctcccccccc
    11221 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
    11281 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
    11341 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
    11401 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
    11461 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
    11521 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
    11581 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
    11641 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
    11701 tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
    11761 aaacacgatg ataagcttgc cacaaccatg gaagatcccg tcgttttaca acgtcgtgac
    11821 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
    11881 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
    11941 ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag
    12001 tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac
    12061 gatgcgccca tctacaccaa cgtgacctat cccattacgg tcaatccgcc gtttgttccc
    12121 acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag
    12181 gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac
    12241 gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga cctgagcgca
    12301 tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgctggag tgacggcagt
    12361 tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt ctcgttgctg
    12421 cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa tgatgatttc
    12481 agccgcgctg tactggaggc tgaagttcag atgtgcggcg agttgcgtga ctacctacgg
    12541 gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc
    12601 ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg tctgaacgtc
    12661 gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg
    12721 cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg
    12781 cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg aggcgttaac
    12841 cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat ggtgcaggat
    12901 atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta tccgaaccat
    12961 ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga agccaatatt
    13021 gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg
    13081 atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag tgtgatcatc
    13141 tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta tcgctggatc
    13201 aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga caccacggcc
    13261 accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt cccggctgtg
    13321 ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc gctgatcctt
    13381 tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata ctggcaggcg
    13441 tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg
    13501 attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt tggcgatacg
    13561 ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca
    13621 gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc cgggcaaacc
    13681 atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct gcactggatg
    13741 gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa
    13801 ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg gcaactctgg
    13861 ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg gcacatcagc
    13921 gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac
    13981 gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg taataagcgt
    14041 tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga taaaaaacaa
    14101 ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga cattggcgta
    14161 agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat
    14221 taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga tgcggtgctg
    14281 attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag ccggaaaacc
    14341 taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt ggcgagcgat
    14401 acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc agagcgggta
    14461 aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc cgcctgtttt
    14521 gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc gagcgaaaac
    14581 ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc
    14641 cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg
    14701 ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg gattggtggc
    14761 gacgactcct ggagcccgtc agtatcggcg gaattccagc tgagcgccgg tcgctaccat
    14821 taccagttgg tctggtgtca aaaataataa taaccgggca ggggggatct aagctctaga
    14881 taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac
    14941 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    15001 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    15061 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    15121 gtctggatcc ggaataactt cgtatagcat acattatacg aagttatgtt taaacggcgc
    15181 gccccggaat tcgccttctg caggagcgta cagaacccag ggccctggca cccgtgcaga
    15241 ccctggccca ccccacctgg gcgctcagtg cccaagagat gtccacacct aggatgtccc
    15301 gcggtgggtg gggggcccga gagacgggca ggccgggggc aggcctggcc atgcggggcc
    15361 gaaccgggca ctgcccagcg tgggcgcggg ggccacggcg cgcgccccca gcccccgggc
    15421 ccagcacccc aaggcggcca acgccaaaac tctccctcct cctcttcctc aatctcgctc
    15481 tcgctctttt tttttttcgc aaaaggaggg gagagggggt aaaaaaatgc tgcactgtgc
    15541 ggcgaagccg gtgagtgagc ggcgcggggc caatcagcgt gcgccgttcc gaaagttgcc
    15601 ttttatggct cgagcggccg cggcggcgcc ctataaaacc cagcggcgcg acgcgccacc
    15661 accgccgaga ccgcgtccgc cccgcgagca cagagcctcg cctttgccga tcctctagag
    15721 tcgagatccg ccgccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct
    15781 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
    15841 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
    15901 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
    15961 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
    16021 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
    16081 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
    16141 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
    16201 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
    16261 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
    16321 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
    16381 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
    16441 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
    16501 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
    16561 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
    16621 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    16681 tcatgctgga gttcttcgcc caccccccgg atctaagctc tagataagta atgatcataa
    16741 tcagccatat cacatctgta gaggttttac ttgctttaaa aaacctccca cacctccccc
    16801 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
    16861 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    16921 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgggggt
    16981 accgcgtcga gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcgt
    17041 cgagataact tcgtatagca tacattatac gaagttatgt cgagatatct agacccagct
    17101 ttcttgtaca aagtggttga tatctctata gtcgcagtag gcggaaatag ctatgtctat
    17161 ttcagccttc ccttccttca gcaacagaaa agccacagtg gtttccacat cagattttca
    17221 tccaaatttc acccagcctc aaaatttcac cctgtttctt aagagtaacc cactcccctc
    17281 ttcttttata ataagagtct caacgccccc aaatttaatc aattgctcat cagaggctat
    17341 gttcttataa tctgccattt cttctgaaga taaacagtat cattttaggc atttgtgaaa
    17401 gagaatcata ttactggtgc ttaagcagtt tttgcttttt tttttttttt taatcttaat
    17461 ccatcttaaa ccagtggagc agaaatattt aaaaatgttt catttcaagc agagttcatg
    17521 ataaattgca ataattgtga tgtgccataa atcccagagc ctatgcattt tgcatttgat
    17581 tcaggattga ggtcaggaaa tttggagaaa tttaaagaaa atgattcatc agcccttttg
    17641 ttctgttggc cagggtcccg ggattcttga gctgtgccca gctgacgagc ttttgaagat
    17701 ggcacaataa ctgtccagtg atgcctgacc atgacagcac agccctctta agccggcaaa
    17761 ccaagaggag aagggttgac attggagtga aaaggacggt agggacagca tctgcatttt
    17821 ttgctaaggc aagggcaaca tttttcagtg ccatgaatcc ccaaggttca gagcaggatg
    17881 ttgaatattc tgtggtgcaa cacgcagatg gggaaaagtc gaacgtactc cgcaagctgc
    17941 tgaagagggc gaactcgtat gaagatgcca tgatgccttt tccaggagca actataattt
    18001 cccagctgtt gaaaaataac atgaacaaaa acggtggcac cgagcccagt ttccaagcca
    18061 gcggactctc tagcacaggc tccgaagtac atcaggagga tatatgtagc aactcttcaa
    18121 gagacagccc cccagagtgt ctttcccctt ttggcaggcc tactatgagc cagtttgatg
    18181 tggatcgctt atgtgatgag cacctgagag caaagcgcgc ccgggttgag aatatcattc
    18241 ggggtatgag ccattccccc agtgtggcat taaggggcaa tgaaaacgaa agagagatgg
    18301 ccccgcagtc tgtgagtccc cgagaaagtt acagagaaaa caaacgcaag cagaagctgc
    18361 cccagcagca gcaacagagt ttccagcagc tggtttcagc ccgaaaagaa cagaagcgag
    18421 aggagcgccg acagctgaaa cagcagctgg aagacatgca gaagcagctg cgccagctgc
    18481 aggagaagtt ctaccaggtc tatgacagca cagactccga aaatgatgaa gatggcgacc
    18541 tgtctgaaga cagcatgcgc tcggagatcc tggatgcacg ggcccaggac tcggtggggc
    18601 gctcagacaa tgagatgtgt gagctggacc cagggcagtt catcgacagg gcccgagccc
    18661 taatcaggga gcaggagatg gctgagaaca agcctaagcg agaaggcagc aacaaagaaa
    18721 gagaccacgg gccaaactcc ttgcagccag aaggcaagca tctggcagag accttaaaac
    18781 aggagctgaa cacggccatg tcgcaggttg tggacacggt ggtcaaagtc ttctcagcca
    18841 aaccctctcg ccaggttcct caggtcttcc cacctctcca gatcccccag gccagattcg
    18901 cagtcaacgg ggaaaaccac aatttccaca cggccaacca gcgcctgcaa tgctttggtg
    18961 atgtcatcat tccgaacccc ttggacacct ttggcagtgt gcagatgcct agttccacag
    19021 accagacgga agcccttccc ctggtggtcc gaaaaaactc atccgagcaa tctgcctctg
    19081 gcccggccac tggcggccac caccagcccc tgcaccagtc acccctctcc gccactgcag
    19141 gcttcaccac ccctagcttc cgccatccct ttcccctgcc cttgatggct tatccatttc
    19201 agagtccact aggtgctccc tccggctcct tctcggggaa ggacagagcc tctcctgagt
    19261 ccttagactt gactcgggac acaacaagtc tgaggaccaa gatgtcatca caccatctga
    19321 gccaccaccc ctgttcacca gcacacccac ccagcaccgc agaaggactc tctttgtcac
    19381 tcataaagtc tgagtgtgga gatcttcaag atatgtccga catctcacct tattcaggaa
    19441 gcgcaatatc cttttatctt tcccctccag aacaagcata aacaaaagcc cagaggaggt
    19501 ctgtgcagag cttaggttcc cacaatgtat aggagggtat atgtcagtat gttttctatg
    19561 tgagatggcg caacgcaatt aatgataact tcgtatagca tacattatac gaagttatgg
    19621 tctgagctcg ccatcagttc agggaggttt agttttgttt gggtttgggg actttgagaa
    19681 gcttcactat gtagcccagg ctggcctagg tctcccaaga tactcagatg acaggcatgg
    19741 accacagtgc acaacttcct taagaatgct gtctttccta ttagttacca tgacaggctt
    19801 gtgacaggct cgtgctgggt gtttctgagt gcagagaaac ttaaaaaccg atcccaacat
    19861 ctctaaaact taggcaagtg cttaattgcc tctagtaact gtttccaagg tgatttccca
    19921 acagtgcttt tttttttctt ctgcctttct tcctgtgtga ctttcctgtc tcctttcttc
    19981 agagcagagg tggggtaggg ggtggaggtg gggagatacc ttgtaaatac tgagttttta
    20041 taaagatggt aagtttcgtg acagaagtaa ggagaaaagc cagttttcag acccgtgact
    20101 ccattaagaa ttctcactgc cagctgcctt tgaggctact gtgggctaga gaggcctgtc
    20161 tacatctcac aaaaagatgc tgtggtgtgg gacagatagc gatgggtgtg gggagtgctt
    20221 gccttctctc tttaatctca agtgagactg tttttaaagg aaatgggcag gtatttattt
    20281 attcctatgg tcggtctctc tctctctctc tctctctctc tctctctctc tctctctcac
    20341 caaggctcgt tcatgggtcc agggcagctg cctgtacgca tgagtgaccc ctgctcagcc
    20401 aaacacagaa atcttttgtc ctaacataca caaagcaaat tatttttgaa aacgagagaa
    20461 cacagttaaa tcccaaactc agctgtataa aaacggcccc tttaatgact cttcccgtcc
    20521 tgaacctgac ttaggtagag acgccactca cgttttcagc agatctgttc cgtattcgtg
    20581 tgattttttt taatccataa cttttttctt ctataaatca agcctttcaa tattttttaa
    20641 aaaaattctt gtggttgttt gtctttgggg tacggggaga atggtagcat ttgggggcag
    20701 gtggaggcat gcctcttgta taataaatta ccagatggat ataaaattta gcaattaagt
    20761 tggctgttac taaattgaga attttgagca atcgtcttga tgactagaaa ttgacatttt
    20821 agtagctaag cccactccag aggctgcatg gatgttccaa gtccacagga tggttgaaat
    20881 atcgtatctc actccttcag ggcagtgtga taacatgagc tgaagccagg agcatgagga
    20941 cttcctgcac cactcagccc agcatctctc ttcccactcc agggaagata ggaacatctt
    21001 gggcaaagac aaagccactg aggtctcaga tctgagtctg agcttgtttg ctggttcaag
    21061 accctaatat tgaatgcacc caaagctaag cctcacagtt gtgttctgta ttcccagcta
    21121 gcaacaagac caaaacagat ctgctcggca ggagaaaaag aaaggaagac gacaggatgg
    21181 atagcttgag tggttgtcaa ccttccctca aggaccttcc tgttaggcta atgtctaagc
    21241 ttagacccct tgcacctcag agcactgcat tttaaaacaa acagtaaagt agcactctct
    21301 ctgtcttctc tctcctatct ctctgtcttt atctctctgt ctttcggtct cccatctctg
    21361 tctctctctg cctctgtcct ctgtctctcc ccaccacctc tctctccctc tttctctctc
    21421 tctctctttc tctctctctc tctctctctc tctctcacac acacacacac acacagttac
    21481 acatgctcat tttggtgact agttaatcta ggctttgaaa tactgaacat acacagaaat
    21541 gtctcgataa taatgccagc tttctaagtt ttggacttct tatcatgatt ggacaattat
    21601 gatcacaccc tttttttgtt agcttgattt tatagggaaa gaaaagtctt ttggttcgcc
    21661 ttaagtgtcc tgtaagctca ctgcaccctt tctatctaaa tcccctgagg aattttgagg
    21721 attggaaggg aaaggagtcc cttcccttcc catacaccca tggctataaa gaaaatgtgg
    21781 aagcatcgtc agggagtgag tgttctgcct cctctgagct gtcatggcag gaggcaaccc
    21841 tgttcacctg aaatacaggt tgctcccctc tggggttgct tccttccttc aggctccaca
    21901 gcctccagcg tcttgttaac agttagtaat tttcccccac attttgggct ctaacctcca
    21961 gctccctgct cttctctatc cattggacct gtgaatggtc agctccttaa aagatcagat
    22021 tttggggaaa ggacaggcaa gccacaacag gaaaccagcc tccctttgca cctgcttcct
    22081 cgctcagggc agcgacgtcc acgactcacc tggctcagac acctgctgct ctacaaaacc
    22141 attttgtgta gtcagtttta gaccaaaaac aacaaaccaa acaaacaaac aaaactacaa
    22201 caactacgaa aagacaaaac aaaacaaacc ccaaatctcc gtcttcgctt ggtaaaatct
    22261 cgaggagcat ttttacttca gtttttgttc tacaaggccc tgcggggaac aatgttaatg
    22321 gagaggaata gccacatcct gggatggtac gtggggacta ccttcactgt gcttttacag
    22381 ttctttaaat ggtcatgact aactttttat ttttaaaact ctggcacact agagtgaacc
    22441 cagggtcttc tgcatgctag gtgaacaact tgcccactga acaatcattc caagacaaca
    22501 tcacttccct cttcaagaac atttctttct ttcttcaaag ggcattcttt ttttctcaat
    22561 gaaatcacta cgataaagaa tagtggtgaa tacctgtttc tcaattgact acgggttctg
    22621 ctgagcacat gagtcactta tatttgaaat actaggctca cagccttgtg gtcattctag
    22681 aaaggagtcc atgtgctcat gtttagtgga acttcagtgc acctgctcaa agagctcatg
    22741 tcttgggtat aaaacactgt gcccagtatc tttgcaaatg aatccgttta atcaaatatt
    22801 aagttttatt caaatcccca gattatcagt tagccaattg tctttcttca ggatgtttct
    22861 tgtccctcat cttctttgca tctcaagaaa aaatagcctt gtgtctgcct tccgcatttg
    22921 catccaccca gctcaagcac aaagttgtca gatgagccat taggagatgc ttggccggag
    22981 gtcctcagcc tcttctctgg gattggcatt tgcaaagaag gtcatgtgat tatttttggg
    23041 ggctgagaaa gaaaaaactg tgtcatggca tagattgata ttgaattata aaacaccggt
    23101 tgaaaatgat gattgttttt ggttgttctt tttggtttgt tgagacaagg tctcactatg
    23161 tagcctaggc tgacctagac tacaattctc ctacctcagc aaccctcact ctgggattac
    23221 aggtgtgcag tgtgaccttt gacattttga caagtcttag agagccaagg aatctaaccc
    23281 atagatgaaa tcctgaatgg ctgtaagcac ctaaccatct aaagaaaaaa agaggaaaag
    23341 aaagtcatta aaacaaataa aactgtacac agctctttga tgatgtcaga ctcctaagag
    23401 tgatgtccgg ctctattctg tcactaaatg agagcttagg tcttttccat ccatttgatt
    23461 ttcttgagaa a
//