LOCUS       KO-first_condition_ready_32233_MGI:97496        21458 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Pbx3 targeting project(s): 32233
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 80473
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     misc_feature    4215..9735
                     /note="5 arm"
     misc_feature    9763..9787
                     /note="B1 Gateway"
     misc_feature    9800..9847
                     /note="Frt"
     intron          9848..10722
                     /note="mouse En2 intron"
     misc_feature    10720..10722
                     /note="Splice Acceptor"
     exon            10723..10895
                     /note="mouse En2 exon"
     misc_feature    10907..11500
                     /note="ECMV IRES"
     gene            11501..14561
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14600..14843
                     /note="SV40 polyadenylation site"
     misc_feature    14847..14880
                     /note="loxP"
     promoter        14907..15426
                     /note="human beta actin promoter"
     gene            15452..16246
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16446..16687
                     /note="SV40 polyadenylation site"
     misc_feature    16704..16751
                     /note="Frt"
     misc_feature    16758..16791
                     /note="loxP"
     misc_feature    10737..10814
                     /note="FCHK std_gpr"
     misc_feature    16807..16827
                     /note="B2 Gateway"
     misc_feature    9736..16857
                     /note="Synthetic Cassette"
     exon            17227..17468
                     /db_xref="ENSEMBL:ENSMUSE00000268194"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000268194"
     misc_feature    16858..17618
                     /note="Critical Region"
     misc_feature    17642..17675
                     /note="Downstream LoxP"
     misc_feature    17619..17698
                     /note="synthetic loxP region"
     misc_feature    17699..21458
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggaaaatag tctattgttt ctggtaaaat taatctgagc gtgtgatatg
     4261 gcagggattg gtggtgtggt cagggagaag agattcccaa ccgtccactg tgctgccatt
     4321 tgcattgctg agagcatgca gaaacatcag agtcatagcc ttgggagttc ccccggcaat
     4381 gaaaaacgct tgtgattcaa gaaatgcctt taaatggttt ccctcccctc atttaaagtg
     4441 tgtctgtgca actgcaggaa aataacaact aggttctgcc caggagtcta gtacctgtac
     4501 agaccaagat actaataatg tcattatggc atccagccgc tctccatcga acctctaata
     4561 aaggtcatta gtgctggctt tcgtgagtga gatggctctg cagccagtgc acagaggaaa
     4621 gttatcagtg ccgtgtgtta gagacaggca ttctgccaac agctttataa tgaaggagaa
     4681 atgtaataaa gcaacagatt gacagtttat gtgttgaaag tgaaggaaat ggaggtgtgg
     4741 agaagcatta tggggaatct ttctagtcat atcttcaatt taaccatcag gggaaatgct
     4801 tcaaaatctt agatttatga atttctagta agcagtttcc cagcatgtaa acaaacctca
     4861 gtgggctgtt ttaggaaggt agatttgggc agtgaagtaa tcatttttat tcctctgatt
     4921 ttccaaatag aaatggacat atatgataaa aatgtcagcc acactctcca cgtcagggcc
     4981 ctaacaggat tacatgtcag ttcttaatgt ctcgctctgt ttttctttgc catgaatggc
     5041 tgcattattg ctcacaggaa cttcactgta atatatggta agagacaagt agctctttat
     5101 ggccagtgat tattcggttt gaatgaaacc tgtgatttat gtccatttaa ctgaaaagcc
     5161 ttgtaaattc ccagtgctgc atttttcctc aatagagaat gagtcaagcg aaagtgacaa
     5221 ctaggttggc gtggaaatgt gcagtgcatt gccgtgttat atttaatata cctgacatga
     5281 aaaacgtgca cacaccacat agccacctta aacaggagtg gaagtgacat tagcttgtaa
     5341 tatgactgcc atattcagtg aattagacac taacatggac ttaaaaggtg gttggtttgg
     5401 agcaggcgtg ggatgggcat tggggccaag ggttgagcta ggcagctgga ttctctgtca
     5461 ttcattcata atactttaag aattagctga ttttctgtta gtatctgtga aagagtataa
     5521 gcctgccctt tcttcatgag gctcaggaaa tgctgatgct gctcagtttg ctcctcccca
     5581 agcgctcttc cccactcttt ccctctttcc tttcttctct ttttgccttt ccattttctc
     5641 ttcatttaag ggtttggtgc ccttagttct gtctctactc ttcccactgt ttctcctgag
     5701 aaccacagcc tctcacctcc ccctctaatc cctcaactcc cacatctcct cttgtcagtg
     5761 gtacacagag tttgtcaata cctttctcta ctttgtttcc agattatttg acaatttaaa
     5821 ccaatttaaa aactaatagt ttcaaataga atcatacaat aattatacaa taatgcaacg
     5881 gcattctgtc tctaggctct ctgtgccata ctctaacagc ttccttacag gcgtcaggca
     5941 gggtttttgt ttgtttgttt cctgtgtttg ggtagaagct tgtttatatt aatcttcata
     6001 atgtggttag atttctcaaa tgatggctga cttgaatttt taaaatggag ttggtattgt
     6061 cattagtcag gttcatagct tctgaccaca gtcttgctta gtacataaca gacacaaaat
     6121 aaaacctgtg caaaattata agagcagctg gaaatgctag aattgttact tgttttaaag
     6181 cctcaacaat tcaagccctg ttgaagatat ctgatctggc tttgtttaca ttattcagct
     6241 aaactactgt cctctgagct acatctgcag catggaaggc aaatgaacca aaaatatgtg
     6301 tgtcagaaaa aaacacagag accttatagg ccaagctttg tgtatagtta gtcttgtttt
     6361 atagtctgct tattagggaa gaaacccaag tttctacaat aatgtttcat ttttggggga
     6421 ataacatatt ctaccaacaa aatggtttcc tcacttttat tcattgctgt tttaaaattg
     6481 tggatggaga tgctgtaggg caggacctag catgtgaatg acatatctgc agtcgctgtg
     6541 atctgaagcc actctggtcc tggacagaca gagcttactc atagcctagt ggctttggtc
     6601 cttgaaaaga gagagccaca aagcttagtg acccaagttt gtatcttctc tcaggtcaag
     6661 ccttgttgta gatagtgttg tgctgctgga ggctagcact gtacaatcca gccaatgcag
     6721 attcctaatc tacttattta taattcaaaa gctcgcctat atattaatga acccttaatg
     6781 agctgttgaa aatgcctgaa ttacagtcag attattgaac tgcaacagcc ccgtaatcat
     6841 accatttgag tgggctgtag gtacttttca catctgcagc ttagtattgt gaagggtgtg
     6901 tgtgtatttg gagaaagctt tataataatt ttgacccgta atttatttat tgccctcaga
     6961 tgcagttgtc tatgcagatg ctctctccca ttatctgtgg ggcaggtgca cagcgtgttg
     7021 aaagacagct aattaggatt tgctgaaaga tatgcaaaaa ctgtgtgctt ataacaaagc
     7081 caagttcatt gttaaacaat gttttactgt gtttgtatga tttgtctcga tcttgtggat
     7141 actgaaatga atgccatggc tataagtaga ctgtaaatgg agtttacaaa acaaaactta
     7201 ataatatgct atgataacca aaagccttga tgtaaaactt tataagccta tttttgtgct
     7261 ccagaataca catgttttga attgaaaatt tttaattgtt gtttcagtgg gaagttacca
     7321 ggaccttttg atgccatggg ctgattcatt ctggcctttc tgcaaatcta tgttcttaaa
     7381 aatgtttata ggtgcccaga ggagtagatg ttgtgattgg aagccatgtg caccctaggg
     7441 aggttagaga gcctagtagt agtagtgcta gagagctagg gagcctgaga aattaaataa
     7501 agacacaaat acaagtattc aaacggaaag taactttaca agtcaagtat tactgcttag
     7561 gcatcccata attatttctt tgcttaataa ggaagtattt tcacttttca aactacagtt
     7621 ttgaaagtaa ctttattatt gataaacagc cttctcaatg ctgcctaatt gcacaatgct
     7681 cttcagttct ttttattgac agggtatgaa gtttctgata ggaaatgatt tttctcatgg
     7741 gatttgatgc acataattta gaaagtaagt gcagaaggat tttgttaaga cagctttctt
     7801 gttcttgagg catgcacatg atggtttact tatggtttta gaaagtaggc agtataagaa
     7861 agtattagag tttttacaat atgttcttat atattttctt gtggccactg ttactgagcc
     7921 tcttagtagc acagtgttgg tctatataca atattaagtg ttatcagatt gccacaggta
     7981 cctagtcact tcatgccttt cgtagtcctg acacatttcc tcttcctcat ggtctgtttc
     8041 cctggtgtat tggtcattga acatctactc ctgtgtgcca cactgtgcag ggaactcaag
     8101 aagtgtaata attttaactc tggctattga tgtactgcta aaggacctta ccacatgcca
     8161 tgagggaaag agcctcatgt gcttaaatat aacggggaaa ttagaaacct tacagaaact
     8221 tgaagtcatc ttttaaaaat atacacatta cagctaggta tggtggctca cacctgtagg
     8281 taatcccagc ctcagaaggg aaaggcaggg ggatcagtct tgagctatgt agtaagacac
     8341 tatcactcac caacacagaa aatatatatc ccccttttta gaagacatat acttatctag
     8401 aaatgaggag aaataagcac ctcccccaca aaaaaagtga gtaaaaattg acttaaaaca
     8461 gataaatata aggagcctaa cttgatttac aattaaacag aactggaagt gcaagaggaa
     8521 gtcatccagg aagctcactt acaccagggc atgagaatga ggtgtgtaag ccaggagttg
     8581 gtcccagtca tatggtctta agacttcaaa cattttagag ttttagaagg aagaaaagaa
     8641 agacgttaag tagaaaacac aaacttagcc aatatggagc agttctagaa caatgaggca
     8701 gttcttttta gtcccccaat aattagcaaa tacatgtttg tctttcttgc tatcagcaaa
     8761 gagattttag cttaaagagt tatctaactt ttggttttcc taaatgaatt tattgccttc
     8821 atatgttata atttttaaat cttacttgtc tctgaactgg caacttatat aattttaaat
     8881 tatggtgact tagttatatt attcagttga aattattttt aagcaaaagt gacagcttta
     8941 aaatgtttgg ggggaggaaa tcaaagttgt gtatttgagc caaagcaagc aagagctctg
     9001 tcatgctgtg aggataattc agggcgtctg tggatgaagt ctgttcgtaa aagaattgac
     9061 ggtacatgta ggttagtgta cgaaatctca gaactggtga tcagctgaat acttggcagc
     9121 ttatagatgt tactgtcatg taagttacac cagacacaga gtgtatgcta ctgtggataa
     9181 acaacaggca cacgagcaag aggcagaatc caaatgcaaa catgtgagat gccaactccc
     9241 cctccctatg gcttgtaact agtgcgggga acctgcttct gaacagattt acagaggtta
     9301 attgggctgg gatagtttag attgtgttac acattaatga aaatgctgat tttgagctgt
     9361 catgactcca gtgcttttta taccatgata attattttaa aactttgtct tctgtgttgt
     9421 tgaaaacatg ttggcagaaa gcctgtgtga cctgattaaa cctatgaatt gtcagctgtg
     9481 atctatagta ctcagaagag gttgctgact ggcatcactt ggaggttctt gatttaaaag
     9541 cagaatgaga ttgaggctac tgtacccagt actcaattgc tgtgtcagtg gcacatttta
     9601 aactctattt ggtgtctttg tgcagtcctc tggcatgtgc ctaacaatac cattttaata
     9661 tgtatttaag tctagttaat cactttatac tgaatgtatc ttgtttttga gattgtgtga
     9721 tagaaagaca tctagaaggc gcataacgat accacgatat caacaagttt gtacaaaaaa
     9781 gcaggctggc gccggaaccg aagttcctat tccgaagttc ctattctcta gaaagtatag
     9841 gaacttcgaa ccctttccca caccaccctc cacacttgcc ccaaacactg ccaactatgt
     9901 aggaggaagg ggttgggact aacagaagaa cccgttgtgg ggaagctgtt gggagggtca
     9961 ctttatgttc ttgcccaagg tcagttgggt ggcctgcttc tgatgaggtg gtcccaaggt
    10021 ctggggtaga aggtgagagg gacaggccac caaggtcagc cccccccccc tatcccatag
    10081 gagccaggtc cctctcctgg acaggaagac tgaaggggag atgccagaga ctcagtgaag
    10141 cctggggtac cctattggag tccttcaagg aaacaaactt ggcctcacca ggcctcagcc
    10201 ttggctcctc ctgggaactc tactgccctt gggatcccct tgtagttgtg ggttacatag
    10261 gaagggggac gggattcccc ttgactggct agcctactct tttcttcagt cttctccatc
    10321 tcctctcacc tgtctctcga ccctttccct aggatagact tggaaaaaga taaggggaga
    10381 aaacaaatgc aaacgaggcc agaaagattt tggctgggca ttccttccgc tagcttttat
    10441 tgggatcccc tagtttgtga taggcctttt agctacatct gccaatccat ctcattttca
    10501 cacacacaca caccactttc cttctggtca gtgggcacat gtccagcctc aagtttatat
    10561 caccaccccc aatgcccaac acttgtatgg ccttgggcgg gtcatccccc cccccacccc
    10621 cagtatctgc aacctcaagc tagcttgggt gcgttggttg tggataagta gctagactcc
    10681 agcaaccagt aacctctgcc ctttctcctc catgacaacc aggtcccagg tcccgaaaac
    10741 caaagaagaa gaaccctaac aaagaggaca agcggcctcg cacagccttc actgctgagc
    10801 agctccagag gctcaaggct gagtttcaga ccaacaggta cctgacagag cagcggcgcc
    10861 agagtctggc acaggagctc ggtacccgga agatctggac tctagagaat tccgcccctc
    10921 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
    10981 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
    11041 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
    11101 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
    11161 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
    11221 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
    11281 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
    11341 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
    11401 ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg
    11461 gttttccttt gaaaaacacg atgataagct tgccacaacc atggaagatc ccgtcgtttt
    11521 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    11581 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    11641 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
    11701 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca
    11761 gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc
    11821 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga
    11881 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca
    11941 tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt
    12001 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg
    12061 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga
    12121 cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt
    12181 taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg
    12241 tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac
    12301 cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact
    12361 acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc
    12421 ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg
    12481 tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat
    12541 tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac
    12601 gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca
    12661 ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga
    12721 tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg
    12781 ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc
    12841 gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct
    12901 gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc
    12961 cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc
    13021 cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg
    13081 cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa
    13141 atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt
    13201 ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga
    13261 ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg
    13321 cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt
    13381 atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct
    13441 cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga
    13501 tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc
    13561 cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc
    13621 cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc
    13681 cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct
    13741 gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg
    13801 cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa
    13861 cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa
    13921 ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc
    13981 tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat
    14041 cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga
    14101 agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt
    14161 agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac
    14221 tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt
    14281 cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg
    14341 gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag
    14401 ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat
    14461 ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc
    14521 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcagggggga
    14581 tctaagctct agataagtaa tgatcataat cagccatatc acatctgtag aggttttact
    14641 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    14701 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    14761 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    14821 tgtatcttat catgtctgga tccggaataa cttcgtatag catacattat acgaagttat
    14881 gtttaaacgg cgcgccccgg aattcgcctt ctgcaggagc gtacagaacc cagggccctg
    14941 gcacccgtgc agaccctggc ccaccccacc tgggcgctca gtgcccaaga gatgtccaca
    15001 cctaggatgt cccgcggtgg gtggggggcc cgagagacgg gcaggccggg ggcaggcctg
    15061 gccatgcggg gccgaaccgg gcactgccca gcgtgggcgc gggggccacg gcgcgcgccc
    15121 ccagcccccg ggcccagcac cccaaggcgg ccaacgccaa aactctccct cctcctcttc
    15181 ctcaatctcg ctctcgctct tttttttttt cgcaaaagga ggggagaggg ggtaaaaaaa
    15241 tgctgcactg tgcggcgaag ccggtgagtg agcggcgcgg ggccaatcag cgtgcgccgt
    15301 tccgaaagtt gccttttatg gctcgagcgg ccgcggcggc gccctataaa acccagcggc
    15361 gcgacgcgcc accaccgccg agaccgcgtc cgccccgcga gcacagagcc tcgcctttgc
    15421 cgatcctcta gagtcgagat ccgccgccac catgattgaa caagatggat tgcacgcagg
    15481 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
    15541 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
    15601 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
    15661 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
    15721 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
    15781 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
    15841 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    15901 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    15961 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
    16021 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    16081 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
    16141 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    16201 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
    16261 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
    16321 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    16381 cagcgcgggg atctcatgct ggagttcttc gcccaccccc cggatctaag ctctagataa
    16441 gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc
    16501 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    16561 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    16621 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    16681 tggatccggg ggtaccgcgt cgagaagttc ctattccgaa gttcctattc tctagaaagt
    16741 ataggaactt cgtcgagata acttcgtata gcatacatta tacgaagtta tgtcgagata
    16801 tctagaccca gctttcttgt acaaagtggt tgatatctct atagtcgcag taggcggaag
    16861 cacactttga tgattgtatg tctgcattat tttcttcttt ttaggaactc cttcctactt
    16921 tatgcataaa tcatttacag ctgaaattta gataatcaga atattctaag aagtcaggtt
    16981 tatctctttt aaagagtcca agttggagtt acatcaaaag ggggagtatt aggggtccta
    17041 ggatgcatgt aaatgtgttt tgaaaacaat tctcatagcc aagcttgttt atcaaatgtg
    17101 agccgtgaag ctctcagcat gagttgtagt acactgatgt tgtttgatca ttttctcaaa
    17161 taaacaaaat actcacactg tttgtcctct gtctctcgct tcttctgtct ttctctctcc
    17221 ttgaaggtct cagcatcaga ggagcccagg aggaagaccc tcccgacccc cagctaatga
    17281 gactggacaa tatgcttttg gcagaagggg tttcaggtcc tgagaaaggt gggggatcgg
    17341 cggcagcagc tgcagccgcg gcagcctctg gaggttcttc agataactct attgaacact
    17401 cagattacag agccaaattg acccagatca gacaaatcta tcacacagaa ctggagaaat
    17461 atgaacaggt cagcagccgc cactctcagt cctgcacaaa ccctctattg ctcttctact
    17521 agaccgtact aaccaattcc gagggccata aggggtaggt gttaacacat agactaaaat
    17581 aagcaacgat gatgcatgta caaaaccaag ggatttgtga gatggcgcaa cgcaattaat
    17641 gataacttcg tatagcatac attatacgaa gttatggtct gagctcgcca tcagttcact
    17701 cacattcatc cctaaccacc acacctgcaa gcccttcctt cttgctaacc tctcacttgg
    17761 tgccctgaag aactctgtcg aattggacct aggcactggc tttgttcata aaaggggaag
    17821 aagcatcctt gctttgtgag cttttgatgt actcagagtt ctaggatcct cctaggaatt
    17881 aattctatgg agtgtgctag gctatgtgga aggagtttct aggatcatct gaggatgagg
    17941 cctcctgttt aattgtgatt gtaacagggc agggcagacc aggcattgtg tgctaaaccc
    18001 ctgatcacaa agactaaaga ttaaaagtct cagcaaactt attggccata cttcattgag
    18061 cctcaagtat ctgagtttaa gctatgttat tgtactatgt gccagcaaca gaaaatcaca
    18121 ttcctagttt aaccctaaat aagtggttcc tggattcagt gtaacatggt aaaatgtggg
    18181 tggtcaattt tcaacatatt taagttagtg atttaaagtt aaaatcctta gtttaacgac
    18241 ttctcactca gcctggattc tcatttgaaa ggtcttttag aagatccact ataatgtcaa
    18301 cttttgaaca tatacaaaag taaagaggtc agttaggatg aatcctgtaa aattgccata
    18361 tttgggggtc aaaacaataa gctggtggag ttgcctgtct ctagtctcag ctactgggga
    18421 cactaaggca agacagttgc ttctgatcag gaatttaagc ccagcctgaa cagcaaagct
    18481 agactactgt ctcacataaa acagcagcac ttcatgcagg cgataccata gagttgttgc
    18541 tcagataagc agtcagcatc ctgccaatct caactgactt ttcccttcac tttttaaata
    18601 tataatttta tccacaaata ctatatgttg gcagttattc aaaatgccca tacactttag
    18661 tgggaacttt ttgtgtgttt gtgaggtgga gagtgttcct gggaacttgt ctttgcctta
    18721 aagaaaagaa agtctaaggg cagttttagg atcacagcag tcttttctca gagatgtctc
    18781 cactgttccc acatctgcat ggcttactta gcaccacggc ctgctagaga ggtctgtctg
    18841 ttacagctca tgaaccagca ctgacacatc agcacccagg gtccacattc tcttagggag
    18901 ctctcttggt ctcacacagt ctgtgcactt ggacagatgc ataagcttat agcaccagag
    18961 agcatgccca ttgccctaac aatccttgtt gtgtgccctc tctgtctctg tctctgtctc
    19021 tgtctctgtc tctgtctctg ttccccctgc ctctcactct tctgctttct ccccctcccc
    19081 tcttcactcc tactctattg atccctggaa accactcttt tattacagtt ctctgaagag
    19141 tttcaattgt atatgttggt ccctaccttt gccttttctt acattctgag catgatggta
    19201 ttaaacaaat gctacatctt tatcccaggg gtgagcattt taaggaagag tagaaagata
    19261 catatagtgt atattaaaat gaaatgcaaa ttaatgctac aaaattttct attttctgag
    19321 cagaactttc tggtattcaa aagtatgttt catttgcaag catacatttt ggaagagaat
    19381 gttcagataa gaactttgga aaaattattg tgtatgaaaa aaaaatccag ttttctgact
    19441 tgatgtgaat aattgagaat aaacaaaata actcatggtt gaaaggttgc cctgagcata
    19501 aacagtagaa aatggttcta atttgtagta taatcctaat tgtataactt tatgctaaat
    19561 agatctatgc atattttcca ggcaactgcc actagtcacc ttgcatagca acgggtgtat
    19621 tgaagccaaa tggcatcact gttctggtca gtcctgactg tccctgtcac agtactcttt
    19681 ctttgcacca gactcactac ttcatccaga tcaccctgtt agaggctggg taaagctggc
    19741 atcaatggaa ggcaaggact cagtcttatc ttataaatgt cctaatagtc ttccaagaga
    19801 ctttagtaac ttcaatgtca actcatacca gagtcagctc actaggggtg tgtgtgtgtg
    19861 tgtgtgtgtg tgtgtgtgtg tgtgcgtgtg tgcgcgcgct catacatttt gccttaaaaa
    19921 atgcacacta tatacaagtt ctgcatagac taaaagattg tgtttgaatt aaatagaaaa
    19981 taaggaatat caaatttaaa tgttataatg gattgtgttt ctgcatacag ttagtagttt
    20041 catggatatt tcaatgtata tttccttcct cgtttttaac atgtcagaag gacacgtata
    20101 gacatgtgga gtctgtgcag cactatgaac agacagatta tgatgaagtg ttgatagact
    20161 tagcaggact gtagtttaca aactttgtct tgtagtctca gctgtttaca gatgaaattg
    20221 tggtgtttca cctcttgtgt tggttttgga cagctttagc acagtgtctc tgccagcgct
    20281 gttttagagg aagagatttc cctttgtttg gacaagaaca aggggctttg gttacttgaa
    20341 tggctaagta gtgttaagta aggggaaggc atggcatcta aagtagatca tattgtattt
    20401 ccattctcta gatgagttga ttgttcagga tcacacatca gtgtcagagt caggcatcag
    20461 atcagttcct gatcataggc catccttgtt tattgagttt tattgtcaca caaagagctg
    20521 gaaaataata caggccatca tagtcaggca tccaaggatc agcccatgtt ggtgtttgtt
    20581 tgaaatggga acaagatgaa aacaagtatc ttaaagactt tccgtatttt gtatctactt
    20641 tgtaacttca tcagaataaa ctatattgaa agcaaaaaaa aaaaaaaaaa ggttttccaa
    20701 aaatcatatt ttcttcttaa aagtgatcac gggaatgcca aatggcagaa cgtttatcag
    20761 gaactaggaa ttaggaaggc cttgttcatc aaggcagatt ctgtgagtcc cagagaaata
    20821 agctacacca gaatcttaag cagtatttac tctgctgtgt tttgcaatga agctgtaatg
    20881 aaaatgtatc tgaggcctac tcagatttag attgccataa aattaacaaa catcaccact
    20941 gcagtaaatt aaagaaccct acaagttaca agcacagcca cagtcacagc tgagcaagct
    21001 acagcaggag caaaatcaaa tctgagctac aaggttcagc tttacatatt aggcatggat
    21061 tatctgtaga attctattct tttcccctcc agaaaatgtt ctttgatctt gtatctctgt
    21121 ttcatagatt tggaaattta atgaattttg ggataatcat ccaacttcag ttataaatgt
    21181 ttctgaaata ttacatactg gctgtgtgtt acagtgaggg ttttttaata ttaccatcat
    21241 tcagcaggaa acaataccat tctactttgg tctcattgat ctgacaccta gttgggattt
    21301 gatagttttc caaggctttc agcaaacacc ttgcaatact tcatcttgag tttattgctc
    21361 ttttttgttg agagacaagc ctccatcttc ttgattagct acaagtctta gaaaatacct
    21421 ttactgtatt tttcaaatat gtacctacga ctagagtc
//