LOCUS       KO-first_condition_ready_44058_MGI:95775        22015 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Gnao1 targeting project(s): 44058
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 194515
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     misc_feature    4215..10168
                     /note="5 arm"
     misc_feature    10196..10220
                     /note="B1 Gateway"
     misc_feature    10233..10280
                     /note="Frt"
     intron          10281..11155
                     /note="mouse En2 intron"
     misc_feature    11153..11155
                     /note="Splice Acceptor"
     exon            11156..11328
                     /note="mouse En2 exon"
     misc_feature    11340..11933
                     /note="ECMV IRES"
     gene            11934..14994
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    15033..15276
                     /note="SV40 polyadenylation site"
     misc_feature    15280..15313
                     /note="loxP"
     promoter        15340..15859
                     /note="human beta actin promoter"
     gene            15885..16679
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16879..17120
                     /note="SV40 polyadenylation site"
     misc_feature    17137..17184
                     /note="Frt"
     misc_feature    17191..17224
                     /note="loxP"
     misc_feature    11170..11247
                     /note="FCHK std_gpr"
     misc_feature    17240..17260
                     /note="B2 Gateway"
     misc_feature    10169..17290
                     /note="Synthetic Cassette"
     exon            17677..17818
                     /db_xref="ENSEMBL:ENSMUSE00001213104"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00001213104"
     misc_feature    17291..17984
                     /note="Critical Region"
     misc_feature    18008..18041
                     /note="Downstream LoxP"
     misc_feature    17985..18064
                     /note="synthetic loxP region"
     misc_feature    18065..22015
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggaaaagag tcaaccagca aatatgccaa gaggtggtct caaattattg
     4261 ttatcaaata tagaacattt taatcatgac ttggatgagt gactgaagct caagtcatgt
     4321 tagattatgc aaaaactaag gaatgctgtg gcttgtatca gtcagagggg tcgggatagc
     4381 ttttgcttca ggcatgggtg gttttaggga ctcacacgac atcactggga cttgattctg
     4441 ttcattcact cagcagttcc atcaaaggcc tctgcagtac atgggactac tggctgttgc
     4501 tacaagcctt gtggagcttt tcttctgctt tccaaaccct aatctcacta cctccaggac
     4561 gcaggcctga gccatcatgg gacagaactc ccctggggtg tacccctgag gacttggagg
     4621 ggccccgctt tcctttccag gacctcagaa catcagacag gcattgcagt tgtgtgcaca
     4681 tgcatatgca taccatgtat atgtatgcac actggggcct ctggaacctg gaggtgaggg
     4741 cctggggttg ggaaagcagc ccagcttctc accctctgcc cataggtcac aaacaggacc
     4801 tgagatgcca gtcaccagca gtatgacccc cacccccacc cccgggaagt gatctaacct
     4861 tctctgtaaa gaggcaggcg ctggtgattt ttatcagggc ctggggctgc tttacagagt
     4921 agaagcaatg cagcaggtgg aacatttagc attaagtggc aaccctcaca gtcgggctgc
     4981 ctctgggcca gaagtgagag aaactgcacc gggaggttga ctttagaatg agaccacgac
     5041 tcctactggc cgccatttgg agtgtcttcc ctgtgatctt cctttttctc ttgggttttt
     5101 gtttttgtat tttggaaaca gggtctcttg taatccaggc tggccttgaa ttcctgatcc
     5161 tcatgccccc atcagtctgg cttgctcagc cacacccatc ttatttctca gaacaattta
     5221 ctaatgagga aacaagactg cttagtgaag tcatttctca gggatgacac ggttagaccc
     5281 tccgcagcag aggcggtatg ggagcagcct ggctgtcact ggaatgatgt gaggagatag
     5341 aagataaggg gaccttatat aatattcgca aagatgtcag cctgaactct gaaagaggga
     5401 gaggaggagg atggtgtgaa caagacaagc ctggcactgc agaccctttc cctgctgtct
     5461 tgtgtggact ccatattctt gtcctaagtg tattctttgc ttgggaatgc tgtaaaacgt
     5521 tagccctgga agcccagttt aaaccagtat ttgccagtgt gacaagttac aggactacta
     5581 ctcatgactg ttgaaccctt gatccaaatt gcatcatttt ctacgttttt cttttgggat
     5641 accccagaaa ccaggctgac acctgatagg caataatctg tgcattctgg ggatggatca
     5701 gatgtggtcg gaaggacacc tggtcagctt ggacacctgg tcagcttgga cacctgggtt
     5761 ctggccttag ctctagctga tctgctgaga gagactgggc aatggcttaa gctcccagcc
     5821 tcagtttctt ctgttaaatg aatataatga aaacctagcc ttcctacctc agtaggtagg
     5881 tgtgggaaat gaatccattg gctagccagg gatctaaaga aaggttttct catttttttc
     5941 cccatgcaac ctctttgcca gagaaatttt tatatgaccc caggtgtgtg tatacatagt
     6001 ataggtaagc aaatcaagag tttaatggtg ataaaacatt tttaaggtta attttaaacc
     6061 aactgtttgg tatccatgta tttttaccat ttgttaaagg ttaaaacaaa gttggatatt
     6121 aatgagatgg atgagcttgt ttgtctttat tcatacagaa ttagatcttg gctgtatttg
     6181 acactgcagg agtctgtcct cactgctcag cattttgatt tcataatcat caatgttgag
     6241 aatgctgctt cacataatat aaatgtatac cagtatatat agcatatatg tgctttatat
     6301 aacacacaca cacatatata tgtgtgtata tgtgtgtata tatatatata tatatatata
     6361 tatatatata tatagtataa atgcagtgca ggagggcaga agtatgtgag gggcaagtct
     6421 agaaactctg tcccgatgcc aagccaaaac taatataatg atttggtttt ttaatggagc
     6481 caaagttagt atctcaaatg gaaattttta aaattaaata ttctaatgca aaataaacca
     6541 aaaggatact aatatatttt ataatatatt ataattttta cttgtacatg ttgaggaatg
     6601 ttgataggaa aattaagttt ttgtactcta catgagtaga aacctttgta tgtactacaa
     6661 taaaaacaca tttcctgaca cacagtagtc atattgtgaa ccatgggtgt ttcgggtgac
     6721 atttgttgtg gtcatgtggc tttatcgcag gcgcagtgtg tacaggctct cactgtgtac
     6781 tcagctggtg tcacagtgaa gttgcagccc acgggatggg tgggccctac cagaggtatc
     6841 tcaggttcat tacacattta attttcaact aattcttcag aaaccttttc ctattatcca
     6901 atttgtcata actcctatat tcagttatag gatcttatgg gatcacagcc cacattcaag
     6961 aagttgggat ctgggaagca cccccagacg agtggcagga ccagggttca gaaatgaagt
     7021 cctggtatct gcgctggctt ctggggaagc ccatcacaca gaaggccagc aaagctcccg
     7081 tgcgcacctc tcaggtcagc tcagcgccag cctcctgagg gacctgctca tggagtcgga
     7141 gtgcccagtg ccttctctac agttgtcagc gtttgctctt aaattcccat gtgtatctca
     7201 cacactttaa tttacttttg agtcttgtga cattttccag atgcatcatt ctgcatacac
     7261 acacacaccc atcctttgca ttcctggaac ttttgcatta aaatagctgt ttttagctct
     7321 ttctctttct gcctccattt gactagtttt gttgctttgt cttcagtttg gacaatcttc
     7381 tgcatgtctg atttgtcatt agttttacct aggacaaatt ttaatgcagc tattgttcat
     7441 tttctgtatc ttccatttcc ctctgaattg tactcacaat cgcctttgat gtcttgaaat
     7501 atttattatt ttgcaataca gcgtcagttc tgccatccat gtctcttcca cctctatttc
     7561 tgttaactga tgcttctctt ggtggtgagc cacacttccc tgcttatctg cagagccagg
     7621 atttttctcc cctggaaggt ggtaccatgc acatccttct gctgagtgtc tagatcttgc
     7681 catgcccatt taaaggttgt tgtcttcctt ctgttattct ttgggggtag ctaagttagt
     7741 catgggcagc tagtttttgt agactttctt tctagcattg tcaggaggac ctttgagggc
     7801 catgagagct atcttaacct agttgctgtg gcctctgatg tccctgctgc aggctgcaga
     7861 agtccccggg gaatgccctc tgacttttca gaggttcatc tcctggcttg gggaagtgct
     7921 gccaagtgtt cggcttccaa ggcccctaga gcacattctc tctctgctac tcttcctttt
     7981 gagctgtgga gtctcatctg ttcagccaaa gaccctcagg aactccacaa agatgccaag
     8041 aaccctttct ctgccatttc ctttctgctc cagctgcctt aaccttcaag tgctgatcct
     8101 cagccccgtg gcccaccagc ccctgtggtg tccctggctt ctccagcctg tgctgtgcca
     8161 gccggtgcct gtattccaca agccacctgc acagccagtg tccccttgtc ccctccactt
     8221 ctccagctct atgcagagtg ctactgtttc ccagggggac tctgtggggc cacaaacctg
     8281 tgggctgttt gtctgttgct ccatcaagag aagcaggaaa gatggctcag tggccagtca
     8341 aaggatccag gttcggttca cttctcagta cccacaattg cctctagctc cagttacaag
     8401 ggatctgacc ctgtcttctg acctgtaggg gcacgagatt catgtgtggt acacttatat
     8461 gcatgcaggc caacactcag aaaaacaaat cttgtaaatt aaaaagaatc tttcgtaaaa
     8521 aataaataca tacatacata aacaaacaaa caaaaacaga cttagtgcca atcaaaccca
     8581 caagctgtct acagagtcat gctgctctgc ttcttaaaga acccacagcc agcactaatg
     8641 ttcactttgc cacaggagtt attgtctatc aatcaactga agactgaata actcaatggt
     8701 agtataccca tacagaggaa tattattcag taataaaaaa ggatgaatta atgagacatg
     8761 ctataacatg agcatatctt gaaaagactt gttttttgtt ttgtttgctt ttttaaatat
     8821 tttatttatc ttgttatgtg agtacacctt cactgttctc agaatagcag atcagaagag
     8881 ggcaccacat ctcattgcag gtggctatga actgtggttg ctgggaattg aaatctggac
     8941 ctctagaaga acagtcaata ctctgaacca ctaagccatc tcttcagtcc cttgaaaagg
     9001 cttgttaaat gaaagaacca gtcacaaaca tacctagtat acaatcttat ggatgtggac
     9061 tgtccaaact atgcaagttt tcagaaatag tgtagattgg tgatagttta ggattgaagt
     9121 ggagtctgca gagtgactgg tgatgagcac agggctactt tgggaagggg ataaaatgtt
     9181 ccacggttag aacgcagttg tggttgccca atctcttgaa tttactaaag caaatatcga
     9241 gctgtgtgct ttaggcgagc agactgtgtg gtagatgaaa tgaacataag cctatgttca
     9301 cgatatgtgt ctgtgtgttc ttctagacag aaagcaggtg atggaggcct ggacaggggc
     9361 cttgatgacg tagaaagcag gctcagctct cacgctgaag cctgggtatg ttgcatgtgg
     9421 taataattct aattcctgtt ctggaaacat ggccgtcaga gctcacacag tctgtcaaag
     9481 cacaaaaaac ctctgatgtt gtccttaaaa catgcttgtt ttttctttaa gactattctt
     9541 tttttttaag attaattaat ttagtgtata gaagtacact gtcgctgtct tcagacacac
     9601 cagaagaggg catcggatcc cgttacagat ggttgtgagc caccatgtgg ttgctgggag
     9661 ttgaactcag gacctctgga agagcagtca gtgttcataa ccactgagcc atcgctccag
     9721 ccccttaaaa ctattcttac agtttgtatg tgatttcctc tttcctaaat ccttgtcagc
     9781 aatgaacatg taccagacaa aagccaccag aggttgtcac actgcctctg ctctgtgatg
     9841 tccctgtgtg gattctgtcg gttgttaaga tccaggagaa cggggtgact acctaatacc
     9901 agctgaggaa gagtttggaa atgcccactg ccggcttttc ttctgatggt atgtctctta
     9961 cacatgtgca caagctagga cggttgttca tttaagccag gcatcaccct actttcatta
    10021 aaagcactgt gagtgagcta tgggcgtggc taccatctcc ccttggagac ctgtcctcac
    10081 ctagcaagtc catccatctg atcctgggtt tgatccacaa gagcggagcg ggtagggttt
    10141 ggttgagaga ctgagactgt atctttcaaa ggcgcataac gataccacga tatcaacaag
    10201 tttgtacaaa aaagcaggct ggcgccggaa ccgaagttcc tattccgaag ttcctattct
    10261 ctagaaagta taggaacttc gaaccctttc ccacaccacc ctccacactt gccccaaaca
    10321 ctgccaacta tgtaggagga aggggttggg actaacagaa gaacccgttg tggggaagct
    10381 gttgggaggg tcactttatg ttcttgccca aggtcagttg ggtggcctgc ttctgatgag
    10441 gtggtcccaa ggtctggggt agaaggtgag agggacaggc caccaaggtc agcccccccc
    10501 ccctatccca taggagccag gtccctctcc tggacaggaa gactgaaggg gagatgccag
    10561 agactcagtg aagcctgggg taccctattg gagtccttca aggaaacaaa cttggcctca
    10621 ccaggcctca gccttggctc ctcctgggaa ctctactgcc cttgggatcc ccttgtagtt
    10681 gtgggttaca taggaagggg gacgggattc cccttgactg gctagcctac tcttttcttc
    10741 agtcttctcc atctcctctc acctgtctct cgaccctttc cctaggatag acttggaaaa
    10801 agataagggg agaaaacaaa tgcaaacgag gccagaaaga ttttggctgg gcattccttc
    10861 cgctagcttt tattgggatc ccctagtttg tgataggcct tttagctaca tctgccaatc
    10921 catctcattt tcacacacac acacaccact ttccttctgg tcagtgggca catgtccagc
    10981 ctcaagttta tatcaccacc cccaatgccc aacacttgta tggccttggg cgggtcatcc
    11041 ccccccccac ccccagtatc tgcaacctca agctagcttg ggtgcgttgg ttgtggataa
    11101 gtagctagac tccagcaacc agtaacctct gccctttctc ctccatgaca accaggtccc
    11161 aggtcccgaa aaccaaagaa gaagaaccct aacaaagagg acaagcggcc tcgcacagcc
    11221 ttcactgctg agcagctcca gaggctcaag gctgagtttc agaccaacag gtacctgaca
    11281 gagcagcggc gccagagtct ggcacaggag ctcggtaccc ggaagatctg gactctagag
    11341 aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
    11401 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
    11461 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
    11521 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
    11581 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
    11641 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
    11701 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
    11761 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
    11821 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa
    11881 ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca accatggaag
    11941 atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    12001 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    12061 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag
    12121 aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc
    12181 cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca
    12241 ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat
    12301 ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta
    12361 actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt
    12421 tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga
    12481 tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg
    12541 gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg
    12601 ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt
    12661 gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg
    12721 tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg
    12781 atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga
    12841 atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag
    12901 cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca
    12961 agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca
    13021 tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg
    13081 tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc
    13141 tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga
    13201 ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg
    13261 atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta
    13321 atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg
    13381 aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg
    13441 atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac
    13501 ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg
    13561 gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg
    13621 tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg
    13681 cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg
    13741 tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt
    13801 tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata
    13861 gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg
    13921 aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc
    13981 agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg
    14041 catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca
    14101 gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt
    14161 tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac
    14221 agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg
    14281 caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg
    14341 tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg
    14401 cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga
    14461 aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta
    14521 ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc
    14581 agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc
    14641 ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata
    14701 ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg
    14761 gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac
    14821 tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg
    14881 acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat
    14941 tccagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac
    15001 cgggcagggg ggatctaagc tctagataag taatgatcat aatcagccat atcacatctg
    15061 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    15121 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    15181 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    15241 ccaaactcat caatgtatct tatcatgtct ggatccggaa taacttcgta tagcatacat
    15301 tatacgaagt tatgtttaaa cggcgcgccc cggaattcgc cttctgcagg agcgtacaga
    15361 acccagggcc ctggcacccg tgcagaccct ggcccacccc acctgggcgc tcagtgccca
    15421 agagatgtcc acacctagga tgtcccgcgg tgggtggggg gcccgagaga cgggcaggcc
    15481 gggggcaggc ctggccatgc ggggccgaac cgggcactgc ccagcgtggg cgcgggggcc
    15541 acggcgcgcg cccccagccc ccgggcccag caccccaagg cggccaacgc caaaactctc
    15601 cctcctcctc ttcctcaatc tcgctctcgc tctttttttt tttcgcaaaa ggaggggaga
    15661 gggggtaaaa aaatgctgca ctgtgcggcg aagccggtga gtgagcggcg cggggccaat
    15721 cagcgtgcgc cgttccgaaa gttgcctttt atggctcgag cggccgcggc ggcgccctat
    15781 aaaacccagc ggcgcgacgc gccaccaccg ccgagaccgc gtccgccccg cgagcacaga
    15841 gcctcgcctt tgccgatcct ctagagtcga gatccgccgc caccatgatt gaacaagatg
    15901 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
    15961 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
    16021 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
    16081 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
    16141 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
    16201 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
    16261 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
    16321 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
    16381 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
    16441 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
    16501 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
    16561 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
    16621 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
    16681 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
    16741 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    16801 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccccggatct
    16861 aagctctaga taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc
    16921 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    16981 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    17041 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    17101 atcttatcat gtctggatcc gggggtaccg cgtcgagaag ttcctattcc gaagttccta
    17161 ttctctagaa agtataggaa cttcgtcgag ataacttcgt atagcataca ttatacgaag
    17221 ttatgtcgag atatctagac ccagctttct tgtacaaagt ggttgatatc tctatagtcg
    17281 cagtaggcgg tgtgaataca agtcaaggct ctgcactgtt cttaggtcac caggagcgag
    17341 cataggctga gcagttggtg tttgctgctc ttgttgcagg tgctttcaag tgtcttcaac
    17401 cccttgttcc tttccctgag tatagactat gaagactata tactgtagtt actcagtcac
    17461 tcaggtaaat ggtttttcat ctcgtttggg tgtttagatt tttagatttt atagcacctg
    17521 tagagcaact cagtagtcca tcatttccat aggaccaggc tctcccattc tccctctgtg
    17581 aacgcagagc ttgcgctggg gcctccaaac cctgtgttgt ccatgtcaag ggaggacact
    17641 gcactcaccg cgggttattt gtggcttttc ctccaggatc atccatgaag atggcttctc
    17701 tggggaagac gtgaagcagt acaagcctgt ggtctacagc aacaccatcc agtctctggc
    17761 ggccattgtc cgggccatgg acactttggg cgtggagtat ggtgacaagg agaggaaggt
    17821 aggcacagcg aaaagaggcc tttgtgacta tgctacatcc tcctgataat ggccaggggg
    17881 ccaccaaccc aagccattgt gccaatcctt gacaaatgca gggacttgtg tgatgggtaa
    17941 agctcagtca atataagcaa aatctaaaac attcttagta tgttgagatg gcgcaacgca
    18001 attaatgata acttcgtata gcatacatta tacgaagtta tggtctgagc tcgccatcag
    18061 ttcaatcaga aaaatattta ctgaacaaaa cccactttgc taggtgccat cttccctact
    18121 gaggagagct gtccttacct tttagaaagg aagcaaccat tggaattgcc tctcattatt
    18181 cacaccaatg ggtatgcttt cttcctttcc tggtaggtgt gaggccctcc ttcctgtaga
    18241 gagtgggcat tctgaaacaa gaactgttct ctaataagtg tataaaagtc ttttatttgc
    18301 aaacatttgc atccactaca aggctaaact acagatactg tggaaaactc cgtaaaatga
    18361 ggactgtgtg agagaggtgt gaaaacaacc tgagaattct ctccaaaagc tggactttgc
    18421 tccccacccc cacccaggga gcagctggag gcctcagagc agctggaggc cttggaacag
    18481 ctggaggact tggagcagct ggcttgcatt gcagtctctg agtagggaac ttagtctctg
    18541 gtgacttgac agataaacat ttccctgcct cacagacgaa agctattgtg ccttctcagg
    18601 gtctgacaaa ttatgtgtca gatagctttg catgtaaaat ttagaatgtt gtgcacaaat
    18661 cctcattcca cagggcagtc acctcagctc aggatggcaa ctccagggcc tggggtagtg
    18721 ggtggctggc acagtgtcta tgccagcctg gggtgggcag cagggtcctg gccgagccca
    18781 tccatctgct gctccccaga ggcaggacag gaagctggca atgtatctat cacaggttct
    18841 tctccagggg gaggcagacc gagggaacat ggaaggcaga atggggagca agggtacctc
    18901 cgcctcttga ggcttgttcc agtcagcgtt ctctgtgcag gactaggcac aaagggaacc
    18961 ttgttcggag aaggctgatg atcagaaagt acctgtgggg cagtgtgggt aagtggtgtg
    19021 cagtggctgc tgaggactgc agcgtctcct tcatcctcct ggccagagaa ctgtgagcat
    19081 gaaaacagcc atcatctgca gaacacctgc tccctgccaa gttctgtgct gagcccatgt
    19141 ttactgaacc ctgagcatag taggtcatca gaaagtctct tgaatggaat tatagtcttc
    19201 ctctccccat tctaagagaa ggggagtacc tggaatttac cctcaaatgg ccagtgtgtg
    19261 cagtgcctat gtgtagcctt atggaaaggg ttgcattggt aaaaggctgt taatcaggct
    19321 tggagaaaac gggccgtctt cttgggaaag tcttgagcat ggcctttctg ttccctgtgg
    19381 tcaaggctgg gtagccttca aaggtagagg gacctgactg agaactagat ttccagcact
    19441 gtcagagaaa gaattcagaa acaggtggac gcggtgagca actgggagaa cactttctta
    19501 cagggcgaag gtacatactc aagatgtaaa tcgggcatct caaaggtcag atcgcgctgt
    19561 ccagtgttgg atacctcacc tcatgatgaa tagaggaagg gggattgttc ttgaattgag
    19621 tggcatgggg cagggacaga gaatgggata ggatttttct cagacaggtt tctcctgttt
    19681 tctgtcctta catgtcctcc atatgtgaca aggtgacacc tgcatagtat aaacaggcaa
    19741 ttttaccagg ctaataatat ggagatgcca ttaaaatgag gcaaagaagt tagtggttct
    19801 cttgatcagg ctgttagccc cagtggtctt gagtggtctt atttctttac ctttgagggt
    19861 catattttgt cctgctgtgg tacagctgtg cagttgcctt aaccgctagc caccttgaag
    19921 tctgcctctc ttttccctgt gtctgtctag gtgacctcat tttcttgaga tagtcaaagg
    19981 aagcttctta tgtagggaag actgcagtgc ctgataccta ggtatgcttt gaaaggtggc
    20041 cgtggaggag gccatggctg aagcagccaa tcagccctga gtatagacag tggctgatag
    20101 tgaccaaaga agatttggct ggcagaggga ggaatgtggt tagagtgtag gcaagggtga
    20161 gaaagttgga agtacaggta agccagattt ggtgagccct ggatggcagc cagtgagtgt
    20221 ggtttcatcc tgctagcaga aagcaaccat tgatagactg tgctagacag gggatgcggt
    20281 aagattcacc caatggcagg cttagggtga catcatcaaa gagcagccaa ggagggaagt
    20341 ggctctggaa gctgtggtaa tagagtacac acgagatctg gggtcagctg agcggtctga
    20401 aaagaaaggt gcacagagag ctgtagtgac aagaggactt gatgatggcc ttggtggcct
    20461 tgggatgcct tgacactggc cagaaataag gactgagacc tgagccctcg cgtgaccagc
    20521 ctggagcaat ggatgagcca gaggcagaga gaaagctgct gggtagtctg ctttcagggc
    20581 caggagcttc tacatagaat gatgagtgag gaggtgtgcc gagtcagagc ataggagctc
    20641 caggatcgtg gaggcctctg cattggtctt ctgggatctg tgttaaatag tgggcattgc
    20701 tatttaagga atactgttta atattaaccc taacaaccac cttacagtag tcgctattgc
    20761 tgtcattgta ttattggaaa gggagagaca gagacagaga cagaaacaga gagagacaga
    20821 gacagagaga cagagaggca gtggcagaaa gcagcagagt ggtgtgcccg ggatccttct
    20881 atataaagca tgtgtgtacg gaggggcatg cttccctaac aggactgcag tccacagcca
    20941 aggctcacat aaacagacag acacatccag ccaacagtgc tcctgagcta ggactggtgc
    21001 acacctataa tcctagcact tgggaaactg aagccggtgc atagtaatct gatactaccc
    21061 agggcaccgc agcgaagtcc tgcccaactc tttcttaatc cataaaagaa tttggaaagg
    21121 gaaccaaaaa aaaagcaccc actgcctttc tcctgatttc tttcctgtct tcctgatgtc
    21181 tatctaaccc attcccttgc atgaccactc cacatcccct gcatgtgcag gcctccagag
    21241 gccgtcttct ctacctagcc tcagactacc tttgccttcc tcttctgtgt ttgccatgac
    21301 tccctctgct tccatctacc ctctgtctag ctgtcaggcc tccctctgta ccatccccct
    21361 ttctcaatat cttcacgttc tctgtgtccc tccccccacc cccaccccct tcactctgta
    21421 caaattgtct tcaactttcc agttgagatt tcccagctcc cttttctcta cctcagctct
    21481 gagctgccct gagccagctg tggaagaaag gacagggaga caggacagtg acagagtttg
    21541 aaggttcact ttgaccgacg aatggaagag cagtctttga tggatctctt gttagtaaat
    21601 tttaccccca tatatgtgcg catatgtaaa catataaaat atatgttttt gtatacatgc
    21661 aatataaatg aatggaacca tagacaacag aagcagaata cgttgaaggg aaaggacaaa
    21721 gcaagtgcct ccagggtgag gcctctttct gcctccctgg ctctccacta gtcagagaag
    21781 ccagaacctc ttccccaagg ctgccataaa cttctctctc tgtgtgaggg atcctttcaa
    21841 agacattctc tgaactgcca tgtctgatag tatgtggctt cagggccctt catttcagtg
    21901 gcaaaagatc ccaaacaggt gttgctgagt ttgcctctca gtcacacata tgtagggcca
    21961 cacccttttt tgtccagtct tgtttctaca gagctgtata tcttcattga gccta
//