LOCUS       KO-first_condition_ready_30926_MGI:95766        21602 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Gna11 targeting project(s): 30926
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 48762
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..9064
                     /note="5 arm"
     misc_feature    9092..9116
                     /note="B1 Gateway"
     misc_feature    9129..9176
                     /note="Frt"
     intron          9177..10051
                     /note="mouse En2 intron"
     misc_feature    10049..10051
                     /note="Splice Acceptor"
     exon            10052..10224
                     /note="mouse En2 exon"
     misc_feature    10236..10829
                     /note="ECMV IRES"
     gene            10830..13890
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13929..14172
                     /note="SV40 polyadenylation site"
     misc_feature    14176..14209
                     /note="loxP"
     promoter        14236..14755
                     /note="human beta actin promoter"
     gene            14781..15575
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15775..16016
                     /note="SV40 polyadenylation site"
     misc_feature    16033..16080
                     /note="Frt"
     misc_feature    16087..16120
                     /note="loxP"
     misc_feature    10066..10143
                     /note="FCHK std_gpr"
     misc_feature    16136..16156
                     /note="B2 Gateway"
     misc_feature    9065..16186
                     /note="Synthetic Cassette"
     exon            16589..16773
                     /db_xref="ENSEMBL:ENSMUSE00000574610"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000574610"
     misc_feature    16187..16966
                     /note="Critical Region"
     misc_feature    16990..17023
                     /note="Downstream LoxP"
     misc_feature    16967..17046
                     /note="synthetic loxP region"
     exon            20853..21602
                     /db_xref="ENSEMBL:ENSMUSE00000452377"
                     /rank="7"
                     /note="ENSMUSE00000452377 5' fragment"
     exon            20593..20746
                     /db_xref="ENSEMBL:ENSMUSE00001216055"
                     /rank="6"
                     /note="ENSMUSE00001216055"
     exon            20387..20516
                     /db_xref="ENSEMBL:ENSMUSE00000298814"
                     /rank="5"
                     /note="ENSMUSE00000298814"
     exon            19314..19442
                     /db_xref="ENSEMBL:ENSMUSE00000298822"
                     /rank="4"
                     /note="ENSMUSE00000298822"
     exon            18432..18586
                     /db_xref="ENSEMBL:ENSMUSE00000574609"
                     /rank="3"
                     /note="ENSMUSE00000574609"
     misc_feature    17047..21602
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaacatat tggagttgtg accagtaggt ctttatgtcc aggcttcagt
     4321 gtcgccatct gtcaagtgat acagtgtccc tgctccatgg gaaaagctgt ggtgttgcgt
     4381 gtgaggcagt gtggcaccaa ccacagtgtg ttcctgtgca tgttcctggg gactccacac
     4441 ttgtgtgtcc cacagcagtg tccactgatc cacggtgttc agggtgtcag gctgccaact
     4501 gcaagggttc tctgtcttta acatggtgtg ctgttgatgt cctcagactc tacactgcag
     4561 gggactgact ggcttctggg tcaccacaag gtctgagggt ggctaagtaa cagaatgtgg
     4621 tcactctagg ggacagttgc ctcatggccg tcctctgggg ctccacatgg tgggcagtgg
     4681 tccctgccat gggtaggttg tcttcagagt tgggcctgct accattgaca gctctgactc
     4741 aaggcctggc ggggcagaaa gcccttgtca caggtgtcaa cacagctctg taatgggtgc
     4801 agcaccaccc cagggctgac atgctagggt ctgcacttcc tgttgtcctg ccctacccta
     4861 gtaaccttgt ttgcgccccc agggtatctc gaagaagggc catgtggtgg acacagggta
     4921 acaggcacgg tccatgtggt ggattcagga gccctgatgg gtgtcattaa gtgatgcagg
     4981 gcagggggac cagccctctg cattcttctt tatgccttgc ctataagaag aacgggattc
     5041 tgttttctgc cttgtagccc aggctagcct cacagtcact atgtagctga tggagacctt
     5101 gaatttctgc gtctcctgcc tccacctgga ccgaatgctg ggacggcagg tgattaccac
     5161 ggagcctggt ttatggcatg ctggggatgg aacgcgggac ctcacatgcg ccacacgggt
     5221 actctaccat tcagctacag ccacagccct gattttcctt gagcctggga cccattatga
     5281 agcccaagct ggtttcacac ttccaaccca cagtgcaagt ttgttccatg ccttccctcc
     5341 agaggtgtaa atctgggagg ccaaaccttt aaactgcctg ggtgtggtgc acgccttaga
     5401 tcccagcact agagaggcag aggcaggcag atctgtgagt tcctggccag cctgggctac
     5461 agaaggagaa cttgtctaaa aaagatctta aacaaaacct tccaacaggt ggacaggtaa
     5521 acctttgctg ttagcaagtg tcagctatga acagagtttc agggcctggt acctatacct
     5581 agggacacgt ggaggcaaga taaggtcaca ccaggtatct ctggctttgt ggagttttat
     5641 aaaagcccat gatagtacag ggggctttgc tgttggtggg gagctactcc gtggggtgca
     5701 taaggctcac tttggctgtt gtacagtagg tgacatatgt cctaactatc agccagctac
     5761 tggtacggcc tgtggctctc tgagaccatc agtgtccaca ccttgggctc cctgggagac
     5821 tttgctacca gcttggagat aagatcatgg ttgcaagtcc agaggctagt gcatgtggcc
     5881 tgtggctgct ggtggcaact gtcactgagc agctgtgact ttcactaccc agggctctgt
     5941 catgagacag gaccaggcgt tacagcccca gcacacaggt gtgctcatgt ctgtcacagt
     6001 gatcatgtgt catcaagcca cttcactgct cctcgactga agttccctga agtgggcctc
     6061 agtgacagtt cctgtacctt gaggctattg gtgctgctgt agcttgggcc acatcagccc
     6121 aggggtgaca cttcctcccc tgactctaag ttggggtgct gagtttctga gggcctacct
     6181 gggaggaagc aaacttggca gtgtacaaac atgtagctct ccctcttcca gcatccctag
     6241 agcctgggtt atgtaggctg ggagggcttt cctacctcac cttcccacat tacagatggc
     6301 acctagagcc atctggcaac ttttgtgggg cagctctgac tcccatactg cctgcattaa
     6361 aacactgagt ccagatgtga acactaggcc agggaaacac agctagagtc tacatacttc
     6421 caacctcatt ctcccaccca tgtattcatc cacccaccca tccacctatt cattcaatcc
     6481 atccatccac tcacccatcc acccacccat caatctatcc acccacccat ccactcttct
     6541 accccctcac acacattcat ttatcccttc ttccaacaat ctgtttatcc accggtttgc
     6601 ctgcctctcc tttacagtgt cttcttcctt gtcctgttgt cctcctatgg tggaggctca
     6661 gtccctctgt gtcctcctgc tctaggtggg gaactctgag cagcctattt taggtctggc
     6721 ttgtggccac tctttcttgc cgtctacttg gtgctagcac agcatggccc ctcttagtgg
     6781 tgccatgggc ttgctgtgtg accagccagc cccagccttg cccttctcca tgggtacctc
     6841 tgacctttag gtggatgtga ctaggtagag ggatagctgc aggaggatgc ctgcagaact
     6901 ccagcatcaa ggcactccag cacagatcct actacggggt acatgggttc attgtttgcc
     6961 ctggcctatt cagaacatgc atgcaaaagc tgatgtctgt ggattcagag ctttgaaaca
     7021 gagattagtg gtgtcagacc ccttagggac accatgtcca gcatcctcag aggaaaggca
     7081 ccagctgggt gttcttcagc tgttgctctg tgacccattg gtaccccagg ttccttagta
     7141 ttgcctgctg tcagcccaca ggtgaactat gactccaccc taagcaggcg aggaaatggg
     7201 gtgtttgtgg tgagagctag cgctctctct ttctctctct ctctctctct ctctctctct
     7261 ctctctctct ctctctctct ctctctctct ctctgccagg aagggggaca ccagccccta
     7321 gggctccttg atgtcactcc tgtctgtgcc catggacact gggcatcatg ttggatccca
     7381 ggcatgactt gagtctggtg catggggatc ctgaacctat atccagcccc cagtgctggt
     7441 tgaccagaca tgacagtggc acacttggct tccagaggca tggccacaga tgaagccaca
     7501 catggtggca tggcccagcc tgggtactga gctgagttgc accttgtcca tagtgccaca
     7561 gggtggcctg aagcccaggg ctagccaggc ttagcttgct gactcacagt gtgaactcct
     7621 ggcctcaggt tcttgtggtg ccttccttgg cctgcgcctg ttgttacagt tgcccttctc
     7681 caccatggat tgtggctcct catcttttgg caactgctga ctgccatcga gtatacactg
     7741 atgaaagagg tgtgatgccc gtgtgtctgt gtgagcacaa ctcacatgag cacagaggtt
     7801 ttagtaattt ttcacgtttt attttacttt ttaaactttg tctgtgttgg gttgtgcaca
     7861 tgagtgcagt acctgtggtg gccagaagag gacggcagat attctggaat tacagctgcc
     7921 tgattggatg attggaaccc agcccaggtc ctcagcaaga ggagccctga gccatctctt
     7981 gagccttgga ttttaatatt taaaagctgt gctgggcagt ggtggcacac gcttttaatc
     8041 ccagcacttg ggaggcagag gcagacggat ttctgagttt gaggtcagcc tggtctacag
     8101 agtgagtcca ggacagccag ggctacacag aaaaaccctg tctcgaaaag caaaacaaaa
     8161 caaaacaaaa caacaaaaaa attaaaagtt gtgactccaa aaggaacata agctctaagg
     8221 aggactatgc gggagaagga gggggacaag tataggtagg gctctacccc tgaccatgcc
     8281 cccagccccc tcactggggg attctaaatg ggggttctgc ctgacaagga cagccctagt
     8341 cctactttta cacttatatt ttatttttgg cttgtgtgca tttgttgtgt tttgtgtccc
     8401 tgcaggtgca tgtatgcatc ttgcatggag gccagaggtt agcgctgagt atccgtccac
     8461 catcccccac tttattgagc acaagcttgc tcattcagct agttgaaccg ggatgccctg
     8521 tatgccctaa gccttgaggt tacaggtggg caccccatcc tcatctctca ggcttcttgt
     8581 gggtttggac tcctgacttg ggccctcatg ccaacgccaa cgcagccttc aatgtgagct
     8641 gcgcacacag atgtgaatgt atgcagtatg caagtgcacc acacatgccg aagagctgat
     8701 aaaaagagct gataaagtct tttcttaaaa gacaggtctt aactgtattg tccaggctgg
     8761 tctgcaactc tccaggctgg ctgcaagaga gcactgttga ttgctgactt aacagctaga
     8821 gttttgggat ttgggaccct ttcagatttg gatgactcac catggtgatc atgcctgcag
     8881 gcatcacaag agctgaagct cttgtctcca acattcggga ccagtgtttg tgggctgtag
     8941 agaaacagct tctgagccac aggccagggg agcgactgcg gtggggcccc aggagagcca
     9001 catacacagc aggtggctgt aagcatgtcc tcagacccac tgcctaaggc ccagcaacct
     9061 ctctaaggcg cataacgata ccacgatatc aacaagtttg tacaaaaaag caggctggcg
     9121 ccggaaccga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcgaac
     9181 cctttcccac accaccctcc acacttgccc caaacactgc caactatgta ggaggaaggg
     9241 gttgggacta acagaagaac ccgttgtggg gaagctgttg ggagggtcac tttatgttct
     9301 tgcccaaggt cagttgggtg gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa
     9361 ggtgagaggg acaggccacc aaggtcagcc ccccccccct atcccatagg agccaggtcc
     9421 ctctcctgga caggaagact gaaggggaga tgccagagac tcagtgaagc ctggggtacc
     9481 ctattggagt ccttcaagga aacaaacttg gcctcaccag gcctcagcct tggctcctcc
     9541 tgggaactct actgcccttg ggatcccctt gtagttgtgg gttacatagg aagggggacg
     9601 ggattcccct tgactggcta gcctactctt ttcttcagtc ttctccatct cctctcacct
     9661 gtctctcgac cctttcccta ggatagactt ggaaaaagat aaggggagaa aacaaatgca
     9721 aacgaggcca gaaagatttt ggctgggcat tccttccgct agcttttatt gggatcccct
     9781 agtttgtgat aggcctttta gctacatctg ccaatccatc tcattttcac acacacacac
     9841 accactttcc ttctggtcag tgggcacatg tccagcctca agtttatatc accaccccca
     9901 atgcccaaca cttgtatggc cttgggcggg tcatcccccc ccccaccccc agtatctgca
     9961 acctcaagct agcttgggtg cgttggttgt ggataagtag ctagactcca gcaaccagta
    10021 acctctgccc tttctcctcc atgacaacca ggtcccaggt cccgaaaacc aaagaagaag
    10081 aaccctaaca aagaggacaa gcggcctcgc acagccttca ctgctgagca gctccagagg
    10141 ctcaaggctg agtttcagac caacaggtac ctgacagagc agcggcgcca gagtctggca
    10201 caggagctcg gtacccggaa gatctggact ctagagaatt ccgcccctct ccctcccccc
    10261 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
    10321 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
    10381 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
    10441 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
    10501 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
    10561 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
    10621 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
    10681 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
    10741 tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
    10801 aaaaacacga tgataagctt gccacaacca tggaagatcc cgtcgtttta caacgtcgtg
    10861 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
    10921 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
    10981 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg
    11041 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt
    11101 acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg ccgtttgttc
    11161 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac
    11221 aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca
    11281 acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg
    11341 catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg agtgacggca
    11401 gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc
    11461 tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt
    11521 tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac
    11581 gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg
    11641 gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg
    11701 tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac
    11761 tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg
    11821 tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta
    11881 accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg
    11941 atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc
    12001 atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata
    12061 ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg
    12121 cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca
    12181 tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga
    12241 tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg
    12301 ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg
    12361 tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc
    12421 tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg
    12481 cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc
    12541 tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata
    12601 cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc
    12661 cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa
    12721 ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga
    12781 tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac
    12841 aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct
    12901 ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca
    12961 gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc
    13021 acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc
    13081 gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac
    13141 aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg
    13201 taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc
    13261 attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc
    13321 tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa
    13381 cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg
    13441 atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg
    13501 taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt
    13561 ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa
    13621 acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact
    13681 tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc
    13741 tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg
    13801 gcgacgactc ctggagcccg tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc
    13861 attaccagtt ggtctggtgt caaaaataat aataaccggg caggggggat ctaagctcta
    13921 gataagtaat gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa
    13981 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
    14041 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    14101 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    14161 atgtctggat ccggaataac ttcgtatagc atacattata cgaagttatg tttaaacggc
    14221 gcgccccgga attcgccttc tgcaggagcg tacagaaccc agggccctgg cacccgtgca
    14281 gaccctggcc caccccacct gggcgctcag tgcccaagag atgtccacac ctaggatgtc
    14341 ccgcggtggg tggggggccc gagagacggg caggccgggg gcaggcctgg ccatgcgggg
    14401 ccgaaccggg cactgcccag cgtgggcgcg ggggccacgg cgcgcgcccc cagcccccgg
    14461 gcccagcacc ccaaggcggc caacgccaaa actctccctc ctcctcttcc tcaatctcgc
    14521 tctcgctctt tttttttttc gcaaaaggag gggagagggg gtaaaaaaat gctgcactgt
    14581 gcggcgaagc cggtgagtga gcggcgcggg gccaatcagc gtgcgccgtt ccgaaagttg
    14641 ccttttatgg ctcgagcggc cgcggcggcg ccctataaaa cccagcggcg cgacgcgcca
    14701 ccaccgccga gaccgcgtcc gccccgcgag cacagagcct cgcctttgcc gatcctctag
    14761 agtcgagatc cgccgccacc atgattgaac aagatggatt gcacgcaggt tctccggccg
    14821 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
    14881 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
    14941 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
    15001 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
    15061 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
    15121 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
    15181 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
    15241 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
    15301 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
    15361 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
    15421 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
    15481 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
    15541 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
    15601 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
    15661 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    15721 tctcatgctg gagttcttcg cccacccccc ggatctaagc tctagataag taatgatcat
    15781 aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    15841 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    15901 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    15961 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgggg
    16021 gtaccgcgtc gagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
    16081 gtcgagataa cttcgtatag catacattat acgaagttat gtcgagatat ctagacccag
    16141 ctttcttgta caaagtggtt gatatctcta tagtcgcagt aggcgggagc accctctgtg
    16201 ctgaccatat gtgatgggtc ttccccgttg gttgcagggg aaggggccag taggaattag
    16261 cagctgacct caggggacca tcacatctgg cccagagact ggtggcaagg ccccaggtgc
    16321 ttctggcatc cggggggtgg ggggtggtac tgagctttca gccatggagg gctcagagac
    16381 tcagggcttg tctgccacag ctgggggaac aggcagcatc ctgagtggta ccctgagtgg
    16441 taccctgtgt tctcaggtgg gagccaagca caatcccttg ggcgggcgag cctgcagaac
    16501 gtctggggta actggcagca tagtgcctca cagtaggtgg gcaggactgt ggggcacgtg
    16561 gtgactggtc aaggtgcgcg gtctgcaggc actggcgaga gcgggaagag taccttcatc
    16621 aagcagatgc gcatcatcca cggggccggc tactcggagg aggacaagcg cggcttcacc
    16681 aagttggtgt accagaacat ctttaccgcc atgcaggcca tggtgcgcgc catggagacg
    16741 ctcaagatcc tctacaagta tgagcagaac aaggtgaggg ccagcccagg cggagtctgt
    16801 gggaggtacc tgcccccgag gcggggtctg tggaaggtat ctgcccttga ggcaggtctg
    16861 tgggaaggac ctgccctcaa gggcggggtc tgtgggtggg acctgcctct gaggggtctg
    16921 tggggtctgt gggtgggacc tgcctctgag ggccgggtct gtgaaagaga tggcgcaacg
    16981 caattaatga taacttcgta tagcatacat tatacgaagt tatggtctga gctcgccatc
    17041 agttcactcc tgcccactga gcttcactgt tgttgctgtt gtttaagtag tattttgaaa
    17101 taactttttt ttttgagaat ttcatattat ttccaactta ttatttctcc tcgcccagtt
    17161 tttcttgatc cttccccacc aggctgtgtg ttcatctgta tgtgtgtatg aatggatgga
    17221 tggatggatg atgaggctca gggtcttgga ctcttaggcc tgaacttacg ctgagccaat
    17281 tccccaaccc ttctgcttta gaaacagggt cttcttgctc agtagcttct tgctcagggt
    17341 cttcaagatc cagtcttgcc atccctctgc ctctgtctcc caagggccag gtattgcctt
    17401 aagaactttg cattagatca tggggccctg tagaggagtc cttgtgtcaa gttggagtcc
    17461 tgtggtcctg gatatggata gaagggaaca ccttgactct cgcttgccct gccctggtgt
    17521 ctctgcagcc tcctgacagt tgttgatgat tctgggccca tttgagcctc acatcttgtt
    17581 ggtgcaggga ggcaggtggg gactgtggaa gctttgcagg cacatcagct ggaagcagcc
    17641 ttctgcccat tccacaaaca ggctgatggg gctggttctg ccaacttact tccttatatg
    17701 agggccaggg tagtcactgt cacaggtggg gttcttccca cttaaggaca ggcagatgtg
    17761 tggaagctgc ccctccaggg ttcagcaagg cccaggcagc ccagtacatg gtgggtgcca
    17821 tgtgagccgt gtagagtcat caaccttatc aggctgggag ccacatctac tagtagccca
    17881 gaacagaggc taccttggga agactaaaga ggagggatcc acggtcaggc tcttgggggc
    17941 tggttagtgg tggtgtgtgg ttcactggct cctctgacct ggggatgtgg aagggccacc
    18001 tcctggtgct ttcaagagcg ggaggcagcc tcagcttatg tgtgccctgt gcagcaactc
    18061 ccctttctgg gcttccaagg atcaggttcc agggtgggct gggttcttgt gggtagagca
    18121 cttgcctggc ctgtatgaag ccctgggttc cctccccagc actagtaaat cccagtgtat
    18181 ggacatagag gttgcagggt gagttcaagg tcagcctggg ctacacaaga tcaaggggaa
    18241 tgcagattat ctgtcccagc cctcataccc acccacacag agtggtcagg gagcatgggt
    18301 ggtgggcggt gggccttgta gccttgtcgc tttctagagg ccttggctcc cctcactgcc
    18361 ctactggctc tgcaggctcc ttgcttgccc agggtccccc cacaggagtt cacagtccct
    18421 tcctccctca ggccaatgca ctcctgatcc gggaggtcga tgtggagaag gtcacaactt
    18481 ttgagcacca gtatgtgaat gccatcaaga cgctgtggag tgaccctggt gtccaggagt
    18541 gttacgatcg caggcgggag ttccagctat ctgactcggc taagtagtga gtggggctgg
    18601 catgcatggc taacactaga gacgtggatg gggtcagagt gggcatgacc taggcccaag
    18661 agcctatcca cagactcact acccacaggc tggccgccca atcagggcag cactctactc
    18721 tcctgcccag atcgtgtgtc cacacagggc agggctggtc tgaccactca ttgcctacat
    18781 gctggcctca gagagaacaa gatgaggctt agagagcagg ggccggactc aggcttctca
    18841 tctgttccat gcctggctgg gagaagagcc cagtcctagc ctgcccggag ggtcaaccag
    18901 ggcagcagag acctgaggac atctaggaga aagtcacagc ttagaggagt ctgtggttga
    18961 gaaggtgttg cagagaatcc cagctgacag ggcactcagg actcagcaag gcacctgggt
    19021 cagtgaccct gagttgagcc tgctgaagtg tctgcctcct atgctgtggc cctggctgta
    19081 gtgggcccca ctctccagct ttccagaggc cttgaatccc ttcctgccat ctatttggaa
    19141 caactgtgac tctagatcaa tggagatagt gtgctctcac cagagccaat acacaggtcc
    19201 cagccctcca gtgccaggtt cccgtctgtc tgtgggtggt tgtgggttct gtgccatgtc
    19261 ccaggtgtcc aatggcccca gtgggctcct gagcagatac ctctacgttg tagctacttg
    19321 acggacgtgg accgcatcgc cacagtaggc tacctgccca cccagcagga tgtgctgcgg
    19381 gtacgcgtgc ccaccactgg catcatcgag tacccgtttg acctggagaa catcatcttc
    19441 aggtgatggt cagcatgctg taagaccgta gggagctgat ggggagagat gcgcaggctt
    19501 ggccgggact ggaaggcagc agcggggatc ctgaggttta gaccccttcc tgggacccct
    19561 gtgaacctga agagcacaag agaaaccctg gagatgcacg gggtgccatg gggatggatg
    19621 tgctttgctc ccattcagaa cccacatcca cagactcggg aggtcacggg gcctgggcct
    19681 gcaggcatgt tgtgcccttt ctgctccctc tttttttttt tttttttttg gtttttcgag
    19741 acagggtttc tctgtgtagc cctggctgtc ctggaactca ctttgtagac caggctggcc
    19801 tcaaactcag aaatccgcct gcctctgcct cccaagtgct gggattaaag gtgttttgtg
    19861 agactcccag tggcaccatg atgctgaccc cagggcagag acatgttcct atttaaaacc
    19921 tttccctggt gggccatgcg tgtgagcata ccacgtccat cactcccttc tactcactcc
    19981 cctgggtggc ctctcaggct tgtgctccct cggcactgag gctgcatctc cccaggagag
    20041 tgtccctacc ccaaacctcc cagccaaggt tcctccttgg ctgccacagc tactccagtg
    20101 agggctgagg cccatgggac atagttaggg accacatggc ctatgacctc aagtcttggc
    20161 aatgtgtggc cagttgggca gctgctgtgt ggctctgagc tatagaaagg tgattcaggt
    20221 ctataaggcc acccaggttc tgggcaaaca ctgccatctg tacaaggggc ttgagcactt
    20281 aagaaacacc agcccagtca tcctgggcct gcagccactt ggccctttgg ggatggggac
    20341 cagttatagg cagcatctag aactgagtgg cctttgatct ccctaggatg gtggatgtgg
    20401 gcgggcagag gtcagagcgc aggaagtgga tccattgctt tgagaacgtg acttccatca
    20461 tgttcttggt ggcactaagc gagtatgacc aagtcctggt ggagtcagac aatgaggtga
    20521 gctggggaca cgcttggggg gctggcaggg gcagggcatg ggtagcctac tcaactaacg
    20581 tgtctgcttt agaaccgcat ggaggagagc aaggccctgt tccgcacaat catcacctac
    20641 ccctggttcc agaactcgtc tgtcattctc ttcctcaaca agaaggacct tctagaagac
    20701 aagatcctgc actcacactt ggtcgattac ttccctgagt ttgatggtga gcactgcccc
    20761 aggtccccac tgtcacccca aggcctggct gccacacgct gggcacctgg gaggccactg
    20821 accaagccgc ccatcactgt gctcatctgc agggccacag agggatgcac aggccgcacg
    20881 cgagttcatt ctgaagatgt ttgtggacct gaaccctgac agcgacaaaa tcatctactc
    20941 ccacttcacg tgtgccaccg acaccgagaa catccgcttt gtgttcgcag ctgtgaagga
    21001 caccatcctg cagctgaacc tgaaggagta caacctggtg tgaccgcggc atggctgcca
    21061 ccagggccag cttcctccgc atccggtcac ccaaaccgag ggacctgggc acacgggggc
    21121 tggggcacat ctctcttccc gctcacttcc tgtccctgtc cctcttcctc aagggcagag
    21181 tggcctcatt tcggccttga ctcaatgtgg cttgaggttt ttttttttct tagaaaacaa
    21241 aaaaaaaaaa aaaaaaaaaa aaaaaagaaa gatacatgaa cagcagtgtg ccctccccag
    21301 agataccccc acagccacaa ggctattaag atcagggcca ctgcagccca agcccaagtc
    21361 ctgagtttga cttctccccc aggaaagctg gaggcttggg gaccatggac tacccatttt
    21421 ctaagttatt gatatcccgt atgccctcag ggctcgcacc atccccctag actcccaaga
    21481 agtgggggtg ggcggcccct gtgcctaccc tgggaagtgc ctaatcgttt tttgttttgt
    21541 ttttgttttt gaagaaaaga gaaatattct ccactctgac ttggcagcca ggtgacacac
    21601 ta
//