LOCUS       KO-first_condition_ready_48771_MGI:95415        22212 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Erg targeting project(s): 48771
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 44824
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     misc_feature    4215..9123
                     /note="5 arm"
     misc_feature    9151..9175
                     /note="B1 Gateway"
     misc_feature    9188..9235
                     /note="Frt"
     intron          9236..10110
                     /note="mouse En2 intron"
     misc_feature    10108..10110
                     /note="Splice Acceptor"
     exon            10111..10283
                     /note="mouse En2 exon"
     misc_feature    10295..10888
                     /note="ECMV IRES"
     gene            10889..13949
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13988..14231
                     /note="SV40 polyadenylation site"
     misc_feature    14235..14268
                     /note="loxP"
     promoter        14295..14814
                     /note="human beta actin promoter"
     gene            14840..15634
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15834..16075
                     /note="SV40 polyadenylation site"
     misc_feature    16092..16139
                     /note="Frt"
     misc_feature    16146..16179
                     /note="loxP"
     misc_feature    10125..10202
                     /note="FCHK std_gpr"
     misc_feature    16195..16215
                     /note="B2 Gateway"
     misc_feature    9124..16245
                     /note="Synthetic Cassette"
     exon            16634..16785
                     /db_xref="ENSEMBL:ENSMUSE00001289148"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001289148"
     misc_feature    16246..16958
                     /note="Critical Region"
     misc_feature    16982..17015
                     /note="Downstream LoxP"
     misc_feature    16959..17038
                     /note="synthetic loxP region"
     misc_feature    17039..22212
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggaaagtaa tatctgaata gatgaccttg aaaaagactt cctttcttct
     4261 ggccctggtt tgcatgtgtg cccctgttac acacagggtg taccagggag tgatagatcc
     4321 ctaggccagg gcagcatttc aggctacagt gcaggcttta agctacaata acccgtggcc
     4381 cagggagttc ttgttcacct catattaaga cgtttgtctt tcaaagtaga gtatcttccc
     4441 ttccccctgt gcaggtgtcc cctgcaggaa aagcatatct gtgagttctg gccactcaac
     4501 ttacattgca acttctcctt tagggcgcct tcacccctcc cccatcttat tttatgtgta
     4561 tagttatttt gcactgtgta tatgcctgtg cactgcttgc gtgcccagca ccggaggaag
     4621 ctagtggaca ccagatctcc caggactgaa cttacagata acggtgagaa gccgtgtatg
     4681 gggattgaac ccggatccta tggaaaagca gccagtgttt ttaacctctc cagcctgtat
     4741 tctcttcaga gtaaccttct cctctgtaag acgtcgacga tgtgtgatcc gctgtagtga
     4801 gggctttccc acgggtcagt gccaggtctt cctatgacat gctgtcacag acaagatgat
     4861 aatgagagca gacgtctcca cccagtcagg gtttgttgag aagccactcc aggggaccac
     4921 acgagacctt ttctgtcaga gttaagagtt ccattaatga aaaacttgta atagtcaaaa
     4981 tcactgcttc atcctgactt cctttgttaa taactaagtg taatagcata aaacatacat
     5041 atttatttgt atctattcat atatttatat ttataagata agtttgaaaa tagagtctaa
     5101 tggcttcatt ttgctgtttg tttggttttt ttttttatga tggaggatgg gatttgaaac
     5161 ccattaacat gaattatgcc tgcaggttat aacatgaaaa tcaaagcaac ttagctaaag
     5221 gctgcccatt gctgcttgat tgcttgtttc gagacacagt ctcactatat agcccaggct
     5281 gtcctcaaac tcactatgta gctcaggctg gccctcaaac ttgtaatctt cctgtttcaa
     5341 catctaccag ccttggcttt gactctgttt tagttatata tagtttgggt gctgtcttag
     5401 tcagggtttc tattcctgga caaaacatca tgaccaagaa gcaagttggg gaggaaaggg
     5461 tttattcagc ttacacttcc acactgctgt tatcaccaaa gaacatcaag actggaactc
     5521 aagcaggtca ggaagcagga gctgatgcag aagccatgga gggatgttct ttactggctt
     5581 gcttcccctg gcttgctcag cctgctctct tatagaaccc aagactacca gcccagagat
     5641 ggtcccaccc aaggggcctt tcccccttga tcaataattg agaaaatgcc ttacagctgg
     5701 atctcatgga ggcatttcct caactgaagc tcctttctct gtgataactc cagcctgtgt
     5761 caagttgaca caaaaccagc cagtacaggc acacaccagc aaatgcaaca tgaggaccca
     5821 tctcatccag atttggtaag ggggtctact tcacttcata gaaaaactcc cagccgggct
     5881 ggtggcacat gcctttaatc ccaggcaggc ggatttctga gttcaaggcc agccttgtct
     5941 acaaagtgag ttccaggaca gccagggtta tacagagaaa ccctgtttca aaaaaaccaa
     6001 aaaaaaaaaa aaattcccat aatccatgga ttatgcactc acacaacaca cagacacaga
     6061 tacacacaga cacacacaca catatataca gagacatgca gacacacaga tatacatata
     6121 tacactcaca gacacagata cacacagatc attgacacag acacacagac actgacacag
     6181 aaagccacac acacacacac acacacacac acacacttag gtgacaatga gaaaagaagt
     6241 agtcaaaccc ccaaagcctt atctctctga ccttcaactt tcaggtgatt aattcctttg
     6301 ctagatagga aaaaaaaact gttagtacct ggtatacagt aggcactcag taagtttcct
     6361 atgtgaatga gacttcacag tcaatcccga tgccgctctc acaccctggg caccacctac
     6421 tgttaaaaaa gtttcctccc aaagaaccaa gtccttcact tctctccccc aagaatctct
     6481 ctctttgaga cacacaacac caaagccttc acactgcact cggctgacag atttttccaa
     6541 aggatcagga aaccctgtct caagtaaaga tagtctgtgg tttgatagta gggtgagtct
     6601 cgtagagata aacattggaa gccaggaaaa tattatagca tcgagcagaa atcctgcatg
     6661 tgtagacagc tctggaaaga cacaggccct gctccatggg aacatagaca gtgactatct
     6721 ttacatcgcc tctgtgttag tacagcggtg agcattctac gtgtagggct atatgttccc
     6781 aggtagtggt aggacctcag ccctgtgtaa ccccagccat tacatgctca gtagctcttg
     6841 ctgtcacttg gcgccagcac tgagctactc tgtggtcctg gaagcattag caagtgatgc
     6901 tgggaagaag tctgtgtttc aagtccaatg ggcctctctt tgcagtgatg accgactagg
     6961 ccatcttttg atacatatgc agctaaagac aagagctccc gggtactggt tagttcatat
     7021 ttttgttcca cctatagggt tgcagttccc tttagttcct tgggtaattt ctctagctcc
     7081 tccataaggg gccgtgtgac ccatccaata gctgactatg atcaggggaa caccagggcc
     7141 aaaaaggggc agtgggtggg taggggattg ggggggtggg tatgggggac ctttgggata
     7201 gcattgaaaa tgtaaatgag gaaaatatct aataaaaaaa atgaaaaaaa aaaaaagaag
     7261 tctgtgtttc aaagtacttg gtgcgctaaa actcttccag ggcaccgtgc cctcagccag
     7321 cccttcctgt ccataattct gtcaaaacct atggatgaga catgaatttc accatgccat
     7381 ttcagctcca tcttgctcgg aggatagccc tgtctacctg cctaatgtgg ttttgctctt
     7441 ctcaaactca aaggtcaagt ccatagcaag cccgaagtcc tgtgactcta caagttacct
     7501 gccaagctgc agctaacaat gctttgaggg aaacaaacaa gggccctagg aaggtggaag
     7561 caaagttttc aacactggcg tgaaaaccta aagagtccat ggatgtctaa tgtttccaag
     7621 agtgtggaaa cacgtgtgtt aggtagaact tgaaaagtgt ttccaaaatt ctactacaga
     7681 taatgtaacc agcacacaga ggtaccactg ctgtggcaat ccaaggttag ccatctgtgt
     7741 gggctagcca ccaactgaga agacggcaac atatttcttt gttctctgat gaagcttgat
     7801 tcgtctttgc taaaaaaaaa atatttatgt gttatgcatg catgtgtata tgtgaatgcc
     7861 tggtctagct tttagtagtc agaaaagtgt ataggatcca ttgaaactgg agttacagat
     7921 ggttgtgagc caccatgtgg gtggtgggag ctgaacctgg gttctctgca ggagcaataa
     7981 gggttgctaa ctgctgagcc ctctctgcat ccacctaaat tgttttcttt acttggtgtt
     8041 aaactatgag acttgtatct ctcaaatatc actttcatgc acacacacac acacacactt
     8101 tttcatagaa agatatcttt gtcttattgc acatacagtg gctgccttac taaagattcc
     8161 tgtggcctgt tttcagggtc agtgggagca actcatccca tccacaccgc cattttcagt
     8221 ccctgtcttg gttcactcag gtcgctggtc tacactcttt atctgcctct cttgcctcag
     8281 ctccacccct caccttctct gccttcctga ctgtgccccc atcaccagga gtccacagct
     8341 ctagatcctg tgtttggcac aagaatagga atccaatggg ggactttgct ggggtctcca
     8401 acgcagcatc cctccctgaa agcattgctt gaactgcatg agtcctagtg tctgctgcta
     8461 gctctgttta ctgtcaccca gcaagaatga acaccagctc tctgttttct gtgagtgttc
     8521 acagacatct gtcacaggag tctggacatg acagtttcag aaggaattgg cgagtgaaat
     8581 agaagccaga cagcagcttg gcttcccact aagtgcttct cctaagcttg gtttcagctg
     8641 cagtgcaggc tgttgagctg agcctgggca cagcatggtc tcagatgtag tgcaggctgc
     8701 tggtctgggt atgggaacag catggtttta cactgtagtg caggctgctg agctgaaaca
     8761 ccaggcccat gttactcatg gttctgcaga tgggacatcc agggacaaga tgctagacag
     8821 ctccatgtca gctaatctgc taggggcttt ctctttgggt tgtaggtagc tacatgctca
     8881 catggaggga gtgaagcaag ggaggagctc tcttcctctt cctctttgtg tgtctcatcc
     8941 atgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgttg tacacattcg
     9001 tgcatgtttc tgtggatgcc agaggtcagt gtctgctgca tttctcagtc actttccacc
     9061 ttattttttt aaggtgaggt ctcttcctga accacagagc taactgatgg gctaaagttt
     9121 cttaaggcgc ataacgatac cacgatatca acaagtttgt acaaaaaagc aggctggcgc
     9181 cggaaccgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcgaacc
     9241 ctttcccaca ccaccctcca cacttgcccc aaacactgcc aactatgtag gaggaagggg
     9301 ttgggactaa cagaagaacc cgttgtgggg aagctgttgg gagggtcact ttatgttctt
     9361 gcccaaggtc agttgggtgg cctgcttctg atgaggtggt cccaaggtct ggggtagaag
     9421 gtgagaggga caggccacca aggtcagccc ccccccccta tcccatagga gccaggtccc
     9481 tctcctggac aggaagactg aaggggagat gccagagact cagtgaagcc tggggtaccc
     9541 tattggagtc cttcaaggaa acaaacttgg cctcaccagg cctcagcctt ggctcctcct
     9601 gggaactcta ctgcccttgg gatccccttg tagttgtggg ttacatagga agggggacgg
     9661 gattcccctt gactggctag cctactcttt tcttcagtct tctccatctc ctctcacctg
     9721 tctctcgacc ctttccctag gatagacttg gaaaaagata aggggagaaa acaaatgcaa
     9781 acgaggccag aaagattttg gctgggcatt ccttccgcta gcttttattg ggatccccta
     9841 gtttgtgata ggccttttag ctacatctgc caatccatct cattttcaca cacacacaca
     9901 ccactttcct tctggtcagt gggcacatgt ccagcctcaa gtttatatca ccacccccaa
     9961 tgcccaacac ttgtatggcc ttgggcgggt catccccccc cccaccccca gtatctgcaa
    10021 cctcaagcta gcttgggtgc gttggttgtg gataagtagc tagactccag caaccagtaa
    10081 cctctgccct ttctcctcca tgacaaccag gtcccaggtc ccgaaaacca aagaagaaga
    10141 accctaacaa agaggacaag cggcctcgca cagccttcac tgctgagcag ctccagaggc
    10201 tcaaggctga gtttcagacc aacaggtacc tgacagagca gcggcgccag agtctggcac
    10261 aggagctcgg tacccggaag atctggactc tagagaattc cgcccctctc cctccccccc
    10321 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
    10381 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
    10441 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
    10501 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
    10561 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
    10621 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
    10681 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
    10741 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
    10801 ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
    10861 aaaacacgat gataagcttg ccacaaccat ggaagatccc gtcgttttac aacgtcgtga
    10921 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
    10981 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
    11041 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga
    11101 gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta
    11161 cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc
    11221 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca
    11281 ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa
    11341 cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc
    11401 atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag
    11461 ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct
    11521 gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt
    11581 cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg
    11641 ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg
    11701 cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt
    11761 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact
    11821 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt
    11881 gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa
    11941 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga
    12001 tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca
    12061 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat
    12121 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc
    12181 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat
    12241 ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat
    12301 caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc
    12361 caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt
    12421 gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct
    12481 ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc
    12541 gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct
    12601 gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac
    12661 gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc
    12721 agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac
    12781 catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat
    12841 ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca
    12901 aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg
    12961 gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag
    13021 cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca
    13081 cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg
    13141 ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca
    13201 actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt
    13261 aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca
    13321 ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct
    13381 gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac
    13441 ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga
    13501 tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt
    13561 aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt
    13621 tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa
    13681 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt
    13741 ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct
    13801 gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg
    13861 cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg gtcgctacca
    13921 ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggggggatc taagctctag
    13981 ataagtaatg atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa
    14041 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    14101 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    14161 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    14221 tgtctggatc cggaataact tcgtatagca tacattatac gaagttatgt ttaaacggcg
    14281 cgccccggaa ttcgccttct gcaggagcgt acagaaccca gggccctggc acccgtgcag
    14341 accctggccc accccacctg ggcgctcagt gcccaagaga tgtccacacc taggatgtcc
    14401 cgcggtgggt ggggggcccg agagacgggc aggccggggg caggcctggc catgcggggc
    14461 cgaaccgggc actgcccagc gtgggcgcgg gggccacggc gcgcgccccc agcccccggg
    14521 cccagcaccc caaggcggcc aacgccaaaa ctctccctcc tcctcttcct caatctcgct
    14581 ctcgctcttt ttttttttcg caaaaggagg ggagaggggg taaaaaaatg ctgcactgtg
    14641 cggcgaagcc ggtgagtgag cggcgcgggg ccaatcagcg tgcgccgttc cgaaagttgc
    14701 cttttatggc tcgagcggcc gcggcggcgc cctataaaac ccagcggcgc gacgcgccac
    14761 caccgccgag accgcgtccg ccccgcgagc acagagcctc gcctttgccg atcctctaga
    14821 gtcgagatcc gccgccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc
    14881 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
    14941 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
    15001 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
    15061 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
    15121 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
    15181 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
    15241 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
    15301 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
    15361 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
    15421 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
    15481 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
    15541 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
    15601 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
    15661 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
    15721 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    15781 ctcatgctgg agttcttcgc ccaccccccg gatctaagct ctagataagt aatgatcata
    15841 atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc
    15901 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
    15961 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    16021 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccggggg
    16081 taccgcgtcg agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcg
    16141 tcgagataac ttcgtatagc atacattata cgaagttatg tcgagatatc tagacccagc
    16201 tttcttgtac aaagtggttg atatctctat agtcgcagta ggcgggtctt tccaggtgca
    16261 catgccatca ggctggtctt tcttgatgca cacaccatca ggctggtctt acggtagatt
    16321 ctgaggatcc aactcaagtc tttgtgtttg tttacataag cgatttactc actgagccaa
    16381 ctccccagtt ctccatcttc ctgccattac tatctctacc ctcatagcct gagctaaata
    16441 tgattatccc tctataccat tatggggggc ttattgcttc aacgtgaatt tgggggacca
    16501 cagcattctg acttttgtcc tgcttcctga ctttccccat gctgcctgtc attggacctc
    16561 tatatcacaa gaccactggg ccctgctctt tgggcactgt tctaacagaa taaattgtct
    16621 ttttctcctg caggaactca cctgatgagt gcagtgtgaa caaaggtggg aagatggtgg
    16681 gcagcccgga tactgtgggg atgagctacg gcagctacat ggaggagaag catgtgccgc
    16741 ctcccaatat gaccacaaat gagcgcagag tgatcgtccc tgcaggtcag aggtcttgag
    16801 attttgttct ggttaacaag ccgtgcttcc caggaaagac agacagacag accacttctg
    16861 tcctttatga gtctctgtca gggacgatat acccagcctt cctgttggag ttgctctctg
    16921 ttctacccac atcactcagc ccccatctac ctcatggcga gatggcgcaa cgcaattaat
    16981 gataacttcg tatagcatac attatacgaa gttatggtct gagctcgcca tcagttcaca
    17041 tcagcactct ctgggcgtga accaggggca ggtaggataa agaatgtcca gctactggtc
    17101 acactttttg tcagagtggt ccaagttatg catagcaaag ctttcgtaag ctccactgtt
    17161 ccctgctcta gataaacaca tctttgtaag gagcaataat gaacgggatc aaaccccttc
    17221 acctccccca ccccaaccca atagtatgat attttctcag gctgtgtcag cttctaaaca
    17281 ggaagttctg tgtgcagaga ctctggcaaa atatgggtct atcagatgtg tcggccagat
    17341 ggggtccctt cagggatgtc actacaagac ttgatagacc tcaaagatgg cttgatgaca
    17401 tcagggcacc agggtcctct tttacagatg tgccttgttt tgccacatat agcaaaactt
    17461 cagaggacac aataatgaca gtcccatgta ccaacaggct caaactgctc tagatattta
    17521 ttggaaggca tatagcatgt actcagacca cacatttggt tttactcaca gctgctgcac
    17581 tgtatactct gaggccacct tacttgccac tgtgcctcag caagggggaa gttcccactg
    17641 ggtggaagct gaccccagtc cacctttgtc attgctggct ggacatgaac tggaggacgt
    17701 ggaagcctaa gggatgctga ccccagccca cctgttggcc ttcctctaga aaagtttcat
    17761 ttcttcacct gaggcaaaga aagtcttagt catgtatgat agaggagcaa gctgaacaga
    17821 gcctgcttga caaacccatc ttctccttgt agaatcaaga tggctatcgc tgctttgtag
    17881 aaccgtgctg ctttgggccg tcttgtattt ctgaagtatc attaggatgc tttaatatca
    17941 tcaaagtctt cttaagagag gtcagacttg agtcttttct cagaggaaaa tgaggcagag
    18001 aattcgtcta gtccaatcag gatgtcagca ggtcttacgc acaggcaccc atccagtgct
    18061 gcctggacag accaaaggat gcatctgcag atggactcca gctggcatga ggtagagggg
    18121 tgtttcttca ttctaaagga agctttcttt cagctgtacc ctcttgggct ggttgcttca
    18181 tgccagggta ggcagttatt cagttaatgg tattggtaaa aagagagagg ggtggatgat
    18241 tttgcaagtt gtgagttttt cagttactgg tcaaggtcta ggtatgtgtg ttcacacgga
    18301 tgaacacact catgcgcatg cacgtgcaca cacacacatt cacacacatt tgaatggtca
    18361 tctggcagag ccatgggatc gcaggactcc aggtggtcag cagcagctgt atccatccct
    18421 tcagcatcag cacaggttat aacccacact ccttacccag actcctttgc agtgtggagt
    18481 attctactca cgttgaagcc ctcagaactg agtccagctg gttcctggaa cttggtggtg
    18541 gttagatgta gtgcctgcct ggtaccaaca gtgtggaaca gaagctttag ttgctctcaa
    18601 ggatttgctc tttctgtcac tacatactga ccttcatatc tgagacagac agagagacag
    18661 agagacagaa acagagacag acagacagag agagagtcaa aatggaggac ctggtaactc
    18721 tatgcctagg tagaaccttt ggacttagag ttcattttga aaaattactc cattaacttt
    18781 gactttgaga tccaccgtct tcagttcact cagtagctaa gaagcccaga aaatactcat
    18841 gaggcatagt cataggcatg ttcttgtggg tgtaaatgcc atgttatatg gatatattta
    18901 cacttcttcc tgtggcaaca taaatttaag aaacattgta tatgtataga catacacaaa
    18961 aatgggacag cctcaacctt tagagtagag gaataggtgt gtgatccaaa ggaagtgctt
    19021 aacagagagg aagcccagtg agtggtatgc atcataagtg tgtaaacatg aagggagccc
    19081 agcatgtggt atgcatcaca tgtgtgtaaa catgaagaga gcccagtgtg tggtatgtgt
    19141 catacgtgtg taagcatgaa gggggaccag cacgtggtat gtgagattgt ataccagtca
    19201 gttcaacata aattgagata ctgttagtaa caagctctcc tctagtaagg atcctagttt
    19261 agcctcacca attttaagcc atcttggaaa acctgcaata tcaaaatcat caaaacaaag
    19321 ccctctttac aataaagtgc tgaatagctc agatgatgtg ttgtgtaaat cccagaatga
    19381 tcatgtgtgc acaatgggga aagatcattc ctcagcctca ttggtgtcag ctcagatttc
    19441 catggtgaca gatggcactg agcccaaaac tagctgtgtc ttcaattctg ggcccctaac
    19501 agcagcccaa acctagtagg tttgactcag atgtctgatc tgcaaggtgt tgccgagact
    19561 gtgccgcaga tccagatgta gaaacattgg attcccagta cacatgaagc caatgttcta
    19621 accaatatca gcatcatgtg tctcacaagt gtctgccatc aaggacagtg tcctctgtgt
    19681 gctggctgtg cctctctgag tctaaggcta ctatttagct tttggtggca tatactaagg
    19741 tgtgctaatg ggctgctacc aaggaaggca cacacattct ccatcatttt gaaaagttac
    19801 aaactgatga ccaaatacaa taggacttct ggttttagca gttgatatta tttttgaaag
    19861 atttttcttt ttatttccta aaaataggtg caaaaatgtt gcacttaaaa actatttctt
    19921 ccacatttta gtttttgttt ttttgttttt gtttttgttt taatttaaca atcatctcaa
    19981 accctttgat atcagcatct cagcgaccta gtggccacac ccgcgatctt cccttgctct
    20041 gcgcatttta agcattaccc aagttccatc ctagtggaca aaggctcccg agagactgaa
    20101 ttctttcttc cctgcctctg attttccatg gaggacatgg aaaacctttg agttgtatct
    20161 cagccagtcc ttcatgccgg attacccagg aagtattcac tgagcggcca ttgctcatag
    20221 gccactctct taggagatga ccaaagaatg ctgaggtcgg gaagatccac ccctgtgcta
    20281 tagatgtgtg gcaggaaaac agacaaaatc ttgaacttag gaatgatgga cgttctggga
    20341 ttcctgagca aggggggtca aaataggtca tgaaagcttc attcatagct gctgcgatgg
    20401 ctttccagcc tgttccatct cccttggcct cctctatctt cctcagccat tccctggcct
    20461 ctggcgtgct gctgtgtatg ccctgcctgt gctgtttggg cagctgtgta tccttccttt
    20521 ctttgccaac aaactgtgct cccccttcaa gacctcttta cactgttccc gtctgcatgc
    20581 cgaactcggt agcaaattaa tcccttttct tatgacacct gaccacctgt ctcttacccc
    20641 acacttatgt tttactctgt attccctaag gcatgtatat gcttatttaa atagaaacaa
    20701 tagcatttga ttctaaagcg gaaatgagat gcttcgggct tgtgacccag actactggcc
    20761 aatcaaaact cagactgagt gaagttaaaa atacacagag aagactaaat gtatatgagg
    20821 aaagagtgaa cacgttccga tggcaggctt ggccacaacg ctgtagtcaa gcctctactg
    20881 tggctttgag cttcctggta gtcaagataa aaagtgtaat tgggaggaaa cgattaaagt
    20941 tgttttatag gagaggtaag cttatctcag cacagaatcc taaaagcagt attcttggag
    21001 actgtagagc cggctgcctc cctgcagcca ttgtggctga gcattgcaac agcacaagtc
    21061 ttctgacaca ttgtgaaaag gctgagtgag ttacactaga gtcagcccag ggaagtcacc
    21121 aacagacact ggggactttg gtggctacac cagacagggc ttgtagttct cagagcagca
    21181 gagtgctgac tgataggcac ctccccaaat atggcattgc tgagctggct agggaaatgg
    21241 gcagaggacc aggagtagag tcccctattc tgggctctgt tagctggaca gcatgttgtt
    21301 atcacaccag catttgttat tgatgaggct gtgtatgtgt agattacaag ttgtgtgaag
    21361 agcacatgat accaatgata attgccacgt agtggcagga atcagaggat catgggagtt
    21421 gcagggtgat tggaaatctc agttcaatag ctccctttaa acccaggaaa aaaatactca
    21481 gaaagataaa gacactgtcc cagagtcagc tgcacagctg aaaggggcca gagccagtgt
    21541 ccagacctgc aaagaacccg cagttaagaa gctataatca gtccttcaca cagcactggg
    21601 ttagaattct ggctttccac aacactttgt ggagcatctt gggcaactct tggcatcagg
    21661 agtcttctcg gtagaatgag aacagtcagt gtttcctgca gtgtatcttg gcagggacac
    21721 atttcccccg aacgctgtgt tctagtgccc ggtcgtgcgt aagcagcaga tgttagctgc
    21781 catcatttga ttttgtaaaa cttcagtctt acatacaacc ccacgtggtt tcctcaacca
    21841 ccatccagcc ataggttcac agataagatg aaccctgact tcagggtttc agttctcttg
    21901 tttggtggtt ggcacagcag gagtccaaca caagcataca gaaacatgat gtgatgggcg
    21961 aggtcaccag gagtgcttgg tagggtgaca aagcaagatg aagattgtgt cagcagccat
    22021 tgtagcctcc taggtaggga gaaggggcaa ggtgagaggg cagtgttacc ctgtaagaga
    22081 ccacgttaga aaggaagctt tctacatacc ttctgtttga gaaaaacaga actctgatga
    22141 taaaacctca aatttatttt aaacaatgac aagaaataaa gagaaataac attcaaatgg
    22201 ccaacgcagg ag
//