LOCUS       KO-first_condition_ready_29388_MGI:88448        22489 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Col12a1 targeting project(s): 29388
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 46816
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     exon            7389..7661
                     /db_xref="ENSEMBL:ENSMUSE00000509909"
                     /rank="14"
                     /note="ENSMUSE00000509909"
     misc_feature    4215..9085
                     /note="5 arm"
     misc_feature    9113..9137
                     /note="B1 Gateway"
     misc_feature    9150..9197
                     /note="Frt"
     intron          9198..10072
                     /note="mouse En2 intron"
     misc_feature    10070..10072
                     /note="Splice Acceptor"
     exon            10073..10245
                     /note="mouse En2 exon"
     misc_feature    10257..10850
                     /note="ECMV IRES"
     gene            10851..13911
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13950..14193
                     /note="SV40 polyadenylation site"
     misc_feature    14197..14230
                     /note="loxP"
     promoter        14257..14776
                     /note="human beta actin promoter"
     gene            14802..15596
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15796..16037
                     /note="SV40 polyadenylation site"
     misc_feature    16054..16101
                     /note="Frt"
     misc_feature    16108..16141
                     /note="loxP"
     misc_feature    10087..10164
                     /note="FCHK std_gpr"
     misc_feature    16157..16177
                     /note="B2 Gateway"
     misc_feature    9086..16207
                     /note="Synthetic Cassette"
     exon            17334..17526
                     /db_xref="ENSEMBL:ENSMUSE00000469041"
                     /type="targeted"
                     /rank="16"
                     /note="target exon 1 ENSMUSE00000469041"
     exon            16627..16893
                     /db_xref="ENSEMBL:ENSMUSE00000468097"
                     /type="targeted"
                     /rank="15"
                     /note="target exon 2 ENSMUSE00000468097"
     misc_feature    16208..17689
                     /note="Critical Region"
     misc_feature    17713..17746
                     /note="Downstream LoxP"
     misc_feature    17690..17769
                     /note="synthetic loxP region"
     exon            21633..21779
                     /db_xref="ENSEMBL:ENSMUSE00000517262"
                     /rank="21"
                     /note="ENSMUSE00000517262"
     exon            20779..20943
                     /db_xref="ENSEMBL:ENSMUSE00000516281"
                     /rank="20"
                     /note="ENSMUSE00000516281"
     exon            20559..20678
                     /db_xref="ENSEMBL:ENSMUSE00000510677"
                     /rank="19"
                     /note="ENSMUSE00000510677"
     exon            20327..20476
                     /db_xref="ENSEMBL:ENSMUSE00000474714"
                     /rank="18"
                     /note="ENSMUSE00000474714"
     exon            18523..18644
                     /db_xref="ENSEMBL:ENSMUSE00000473834"
                     /rank="17"
                     /note="ENSMUSE00000473834"
     misc_feature    17770..22489
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggatggctt atcacaaatt tctcattttc caggagctgt tattctagac
     4261 aaaagctgga gtgaaagttc actgcccctc tggccacatg tgtcttctga acaaacagct
     4321 ccatagcatc atcattatca gcagcagcag cagcagcagc agcagcagga gtagcagcag
     4381 caagaacctg gggaggattg agagggtaac agaaggagag tcgggcatgt gtggaggaaa
     4441 gagataagag taaagcgaca tagaggaatg agtcgcagga cagatttaaa agggctttag
     4501 ggactcttag tatttagaca cagtctcata tcttctgact tgagcagaga tgagctttgc
     4561 ctctcaaagg tacatttcta tgtacaccat gttttattgc taaggcacta ggaagtgaca
     4621 tagctggctg gtgacacact cagctggctg cacacttaac ttcaccacta gccgcatgcc
     4681 agccagtatc ctgcctgttt cactacagtt tcctaaatcg tttcttcaat ttttggtaat
     4741 ctgatgattg ttaaaaacaa acaaacaaac aactttgtag attgtaggtc tgataaaacg
     4801 ttctcatctt ggattatctt tttctcaaaa atccatgtgt tacaaagtac cttgtatttc
     4861 attagagtac atggcttcct actttgtaga caaagcagca gtcagtctgc ccgaaaagag
     4921 aacaaactta acttcgagga gtactcatcc tcctgactga tagaagaaac cagcacacac
     4981 ctttaatccc agcacctggg aggcagaggc aggtggattt ctgagttcga ggccagcctg
     5041 gtctatagag ttccaggaca agccagggct acacagagaa acactgtctc gaaaaacctt
     5101 aaaaaaaaaa agaagaagca tttgtctttt cagtcttggc ttctgaattg aggtgctttt
     5161 actctatggg gtctgacctt cgtctttagt gtggaatgta tgtctgcaga taatattcat
     5221 atacctcagt cagaaactct gatctaaagg cctgtttctt gtgactcgaa atcttctctg
     5281 aggccatttc agtcattgct ggtgtgcttt cagtgagaat gaaaacactt tttagttgct
     5341 atttcatttg tatcttaaag agtttttttt ttccaaatta atctcatgtc attaatgcac
     5401 ttgaaagatc tttactgtga ttttgctagc attttgttct agctttactg gtcttttgag
     5461 tgttaaaata gttggttaag cccatgggaa gacatgtcta gtgaactgaa atctttttgt
     5521 tctaagcaca gaaacatctc tgtgaatata tttttgtctc aaccacaatt ctcttcctgt
     5581 tatttttctc atagtgaaat cacttgcgtg tgtttaggag attgatgcct gttgtcatgg
     5641 aaacaaacaa tgctacaagt tcagtgctgt agaaagtggg ggagaaaaat agactaaaaa
     5701 acatagccct tagggaaagt agaagttggt gattactgtg gtattaattt ggaagaaaat
     5761 tcccatattt taagctattt ttgtatgttt gggggatatc attaatacat taactggctt
     5821 attgaagtac aaagtgacta tcaagtgagc tcattttccg gtagcccttc tgactgcagg
     5881 ctaagagatt cagaagcaaa ggcacatgtg gaaaaatgat ctatggtagg caaaggaaga
     5941 gtgcattgca gacttgaaac ttaatggggt tgtgggaaat cagtcaccaa aacaagtaga
     6001 aaacaacgaa caaccaaacc aagccaagaa gctagtgatt tgaagatgca ctttcatatt
     6061 ttgcaagtaa agaaaatata gggttattcc catgcaagca gcaaacactt cccctataaa
     6121 ggaaaataag tcactatgtt cttggaaata agtgcttttg ggtccagtga aacatgcaga
     6181 taatttcctt ctagagagga gaagtcctgt ttgctttcaa agttatttat aatggaatac
     6241 gaggttccca ttagggtctg gtgtgcattc cagcttctct atttatcttt ccctctaacg
     6301 tatagcatgt atgaggagtg tgtgcaccaa ctttgttgtt gttagtcatt atctttactt
     6361 ttggctgatg cttaattgtt tataagtagt tctaaaatgt taattttcac tctgagttga
     6421 gaaaatgaca gatttcaaaa atctagtatg cacgctatca tttacatcaa tgtaaaacaa
     6481 ttgctagcat aatacatact caggaggtgg atagttctat atttatgtca cggtgtatca
     6541 taaactctag taaaagcagc caatataaag tgaccagtat atttatatta catttaaaaa
     6601 atggtaagtg gggattttat tagaaatcat ctgcgcctat tatcaagttg agatttctac
     6661 atttttattt aatagtatat gtataatgta cattagtata tataatagta tgtatatata
     6721 tgtagacagt tttgataaga tataataagt ggtgtactct atttcaatga caaatcactt
     6781 ttaagttttc caaagtattg gtgattttta gtttcattgg agttaagcta tgttgaaagt
     6841 ttgtattttt actcttttga attgaatatg caagtaaaag tctcaacaga aaacatttgt
     6901 gggagtttag gtttgcaagt ggtgtacaat tgtatttact ataattcata taacaaaact
     6961 gagacgatta agactaaaca gaaaggaaat aaaacctggt aagaacttta agacctttgt
     7021 gaacagtggg ctatgtccta aatcacactg aagtgcgata aaatattgac atttagacat
     7081 atttttctct ttggaaagaa tgttaaaata tgcatttttt tcaaattaag atgtgagccc
     7141 tggcctagag gactctgcac tgaaaatgag gacctgggca aggctgatag ttaggaatcg
     7201 atcaccccgg agtgttcttc caccagtttg tcccattgac tgaaaacagc cccaaagctc
     7261 agaaagaatg gttagcttta gataggttga ctttgagtcc agtatgttag gatgtctacc
     7321 gagatgaatg aatacttaat tatccagctg acagttaaaa aatgctactc ttttactctc
     7381 cattccagaa cgtggctcac ctcagaacct agttaccaaa gacatcacag acacatcaat
     7441 cggagcctat tggacatctg ccccagggat ggttcgaggg tacagggtct catggaaatc
     7501 gctttacgac gacattgagg ctggagagac aactcttcct ggagatgcaa ttcatactat
     7561 gatagagaac ctgcagccag agaccaagta caaaatttca gttttcgcaa cttacagcag
     7621 tggagaagga gagcccgtga ccggagatgc cacaactgaa tgtaggtaac atcccaggtg
     7681 tgttgggatg catgttggtg ctacctaatg gctagtcagc tcaagtgaca cgcagtttaa
     7741 ccactgaggt tgttttcttc acttggtagt aactgacgtt caaattagat ttggatttga
     7801 aaaatgatat cacttggaca attttgaatt ctttccagct atatgtattt ttttttcatt
     7861 ttatttttct gtgaaaagat cagctaaata ggacactcaa ctagtgaaaa gacagcttcc
     7921 atgtagacaa gtagaattta ggagcatttc tggttgtata taatcttgtt caagagcctt
     7981 tgcttttctc caagagtcgg tttcctaatc tgtagaatat ggacaatcca tttctactct
     8041 tacagtgcca caggggtgaa aggaaatgat agtaaagagc acaacaatta aacacgtatg
     8101 acagtgcata cgttcagcaa tgtcaagaag tgtacttctt tgttggggaa agctgtgtgt
     8161 ttatgcaggc aaagaaggat acctggtgca tatctgtgct ttttgatcac tattgtttat
     8221 tttatattat atatatatat agccagcaag ggctagctag gattctgctt gcctctgcct
     8281 cctgagtaca tgtatcacca ggtctggtta gttgagactg ctggtactct tacagggttc
     8341 accctcctcc ttagcttctt tcagccttcc ttaattcaac aacaggggtc agctacttct
     8401 gtccattgtt tgggtacaaa taactgcatc tgactttcag atgtttgttg gcgtcttcaa
     8461 gagggcagtc atgatagatt cctttttgtg agctctccat agcctcagta atagtgtcag
     8521 gccttggggc ctctccttga gccggatccc actttgggcc tgtcactggg ccttcttttc
     8581 catttccatt cttgtaattc tttcagacag gaacagtttt gggtcagaga tgtgactgtg
     8641 ggatggcaac cccattttga agctgtttca ttatgattct tatccatctg aacataaaaa
     8701 ttatctttga aaattaagaa agacaagatt ttgttttctt cagctttagt gccttctaaa
     8761 agtgcttagg agtcgttgag tcttaagagt cttgcattga attgaatgat ctggcaagta
     8821 aagccattgt aagaaagggg atataggttg tttgaagata tttggtgatc tatttttgag
     8881 ggttattcat aatatgaaat attaatgaaa gagctagctt aataatgatg taagctatgt
     8941 gttagatagg tattaatcta cagcactttc taaaatactt gatgtgtgtg ctttcatctg
     9001 ctagcttttg agatgtttta atgtgtcaga attgcctatt ttgtgttcat tgtcttatac
     9061 acacagcaat gagagggttt gtgtcaaggc gcataacgat accacgatat caacaagttt
     9121 gtacaaaaaa gcaggctggc gccggaaccg aagttcctat tccgaagttc ctattctcta
     9181 gaaagtatag gaacttcgaa ccctttccca caccaccctc cacacttgcc ccaaacactg
     9241 ccaactatgt aggaggaagg ggttgggact aacagaagaa cccgttgtgg ggaagctgtt
     9301 gggagggtca ctttatgttc ttgcccaagg tcagttgggt ggcctgcttc tgatgaggtg
     9361 gtcccaaggt ctggggtaga aggtgagagg gacaggccac caaggtcagc cccccccccc
     9421 tatcccatag gagccaggtc cctctcctgg acaggaagac tgaaggggag atgccagaga
     9481 ctcagtgaag cctggggtac cctattggag tccttcaagg aaacaaactt ggcctcacca
     9541 ggcctcagcc ttggctcctc ctgggaactc tactgccctt gggatcccct tgtagttgtg
     9601 ggttacatag gaagggggac gggattcccc ttgactggct agcctactct tttcttcagt
     9661 cttctccatc tcctctcacc tgtctctcga ccctttccct aggatagact tggaaaaaga
     9721 taaggggaga aaacaaatgc aaacgaggcc agaaagattt tggctgggca ttccttccgc
     9781 tagcttttat tgggatcccc tagtttgtga taggcctttt agctacatct gccaatccat
     9841 ctcattttca cacacacaca caccactttc cttctggtca gtgggcacat gtccagcctc
     9901 aagtttatat caccaccccc aatgcccaac acttgtatgg ccttgggcgg gtcatccccc
     9961 cccccacccc cagtatctgc aacctcaagc tagcttgggt gcgttggttg tggataagta
    10021 gctagactcc agcaaccagt aacctctgcc ctttctcctc catgacaacc aggtcccagg
    10081 tcccgaaaac caaagaagaa gaaccctaac aaagaggaca agcggcctcg cacagccttc
    10141 actgctgagc agctccagag gctcaaggct gagtttcaga ccaacaggta cctgacagag
    10201 cagcggcgcc agagtctggc acaggagctc ggtacccgga agatctggac tctagagaat
    10261 tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
    10321 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
    10381 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
    10441 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
    10501 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
    10561 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
    10621 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
    10681 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
    10741 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca
    10801 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc atggaagatc
    10861 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
    10921 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    10981 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
    11041 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
    11101 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta
    11161 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
    11221 atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
    11281 cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc
    11341 cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
    11401 tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
    11461 ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
    11521 ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
    11581 gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
    11641 ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
    11701 gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
    11761 tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
    11821 gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
    11881 cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
    11941 atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
    12001 gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
    12061 atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
    12121 atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
    12181 gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc
    12241 acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
    12301 gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg
    12361 aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg
    12421 gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg
    12481 gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
    12541 gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt
    12601 acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct
    12661 ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc
    12721 agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
    12781 ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag
    12841 tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc
    12901 cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat
    12961 ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg
    13021 tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt
    13081 gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga
    13141 tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
    13201 cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
    13261 aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
    13321 atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa
    13381 ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
    13441 ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc
    13501 tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
    13561 accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
    13621 cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
    13681 cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga
    13741 tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
    13801 gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc
    13861 agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg
    13921 gcagggggga tctaagctct agataagtaa tgatcataat cagccatatc acatctgtag
    13981 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    14041 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    14101 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    14161 aactcatcaa tgtatcttat catgtctgga tccggaataa cttcgtatag catacattat
    14221 acgaagttat gtttaaacgg cgcgccccgg aattcgcctt ctgcaggagc gtacagaacc
    14281 cagggccctg gcacccgtgc agaccctggc ccaccccacc tgggcgctca gtgcccaaga
    14341 gatgtccaca cctaggatgt cccgcggtgg gtggggggcc cgagagacgg gcaggccggg
    14401 ggcaggcctg gccatgcggg gccgaaccgg gcactgccca gcgtgggcgc gggggccacg
    14461 gcgcgcgccc ccagcccccg ggcccagcac cccaaggcgg ccaacgccaa aactctccct
    14521 cctcctcttc ctcaatctcg ctctcgctct tttttttttt cgcaaaagga ggggagaggg
    14581 ggtaaaaaaa tgctgcactg tgcggcgaag ccggtgagtg agcggcgcgg ggccaatcag
    14641 cgtgcgccgt tccgaaagtt gccttttatg gctcgagcgg ccgcggcggc gccctataaa
    14701 acccagcggc gcgacgcgcc accaccgccg agaccgcgtc cgccccgcga gcacagagcc
    14761 tcgcctttgc cgatcctcta gagtcgagat ccgccgccac catgattgaa caagatggat
    14821 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
    14881 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
    14941 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
    15001 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
    15061 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
    15121 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
    15181 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
    15241 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
    15301 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
    15361 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
    15421 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
    15481 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
    15541 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
    15601 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
    15661 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    15721 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccc cggatctaag
    15781 ctctagataa gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt
    15841 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    15901 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    15961 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    16021 ttatcatgtc tggatccggg ggtaccgcgt cgagaagttc ctattccgaa gttcctattc
    16081 tctagaaagt ataggaactt cgtcgagata acttcgtata gcatacatta tacgaagtta
    16141 tgtcgagata tctagaccca gctttcttgt acaaagtggt tgatatctct atagtcgcag
    16201 taggcggttc taactctgta cttttgaact ttagcattcc ttgtggcatt atgaccagag
    16261 gaattcttta cagataaaac ataacaaaaa atttaagacc acatctttgg agattgactt
    16321 ttcttttttt ccccaaatct ctagaatgca gataggaaat ccctgcaata tgaactttta
    16381 tttttctgta ttaactggat aaagcgagag cattctactg aggccacgct gagcttaggc
    16441 tcacatgcca tcatgacacc acgactgcaa cattaagcaa gacatctgtg tccacactta
    16501 gaatctagag ttccttccta cacgaaggga gaggcagaca aaaatgtttt cctttaacat
    16561 gctgtacatt attattatta ttatcatcat cactattatt tattgttatt attttttatt
    16621 tcacagtatc tcaagattcc aaaatcttaa gagtggatga agaaacagaa cacacgatga
    16681 gagttacctg gaaagcagca ccggggaaag tcgtcaacta ccgtgttgtg tatcgccctc
    16741 aagggggtgg gaggcagatg gttgctaagg tgccacccac ggtcacttca acagtgctaa
    16801 agagacttca accccagacc acatatgaca tcacagttct cccaatgtac aagacaggag
    16861 aaggaaagct caggcaaggc tcaggaacaa caggtataaa acaatgataa atattttaat
    16921 tgaatctggg gctggggggc tgtgtctgag ttatagagta tttagccaac aggaatgggt
    16981 ccttggattt aagctctaga acactatagg caaatattta taattgtaat aaactaaatt
    17041 atatttatta gatggggtgt ctaattagtt tattacttgt tttgcaaatc tagttccttg
    17101 tgaactgatc tttctgctaa tgtatcaatg caaaactcac ttgaattgtg ccaattgtgt
    17161 gtgctgctct tgcagaactc aatttcccag ggcttttatt gagcctgaca ttttatgcta
    17221 gtagtagact tgacccttct tatacataca cattttttct tgtgggagtg aatccaaaac
    17281 acctaagcct atcttatcca tagtattgtt cttaaaatct tttattttta cagcgtctcg
    17341 atttaaatcg ccgagaaacc tcaaaacttc tgacccaacc atgtcaagtt tcagagtgac
    17401 ttgggagcct gcccctgggg aagtgaaagg ttacaaagtc acattccacc ctacaggcga
    17461 tgacagaaga cttggggagt tggtccttgg accatatgac aacacagttg tcttggagga
    17521 gcttaggtag gaatgaactg tatttgtgct aaggactgta tcacttaggt aaatttaaat
    17581 aggctttata caaagaacaa cctcacgtgt ttgcagaaaa cagaaaagaa ttaagtctga
    17641 caaacagctt gtattgttta gataactttt gttactaagt gatcagggag agatggcgca
    17701 acgcaattaa tgataacttc gtatagcata cattatacga agttatggtc tgagctcgcc
    17761 atcagttcag ttgcagtcat gtgaaaatgt gtggccaagt gactcattct gagacactcc
    17821 cctgcccccg ccagggtgga tgtccctgca gggaagcaca ctggagctca aaggccagag
    17881 tctgtgcttg tggccagctg gcagaagagc tttgagttga ttagaggtct agttttagac
    17941 agtgagatgg aaaacaaagg agtgtgaact tgaacagcac tccacattca tccagagata
    18001 ccaatgggtt actgtacatt cctcttggaa actttgcttg ctcaagtatt cattcaatag
    18061 tgttggaacc tgtataatgc ttgctgttca ttgtaacttg agtaggtagg atgaaatttg
    18121 gaaggtcact gtttgacatt gcccagtgct gagtgtctta ggtcattggg cagacacaga
    18181 acatttcaat cattctaaag ccggcatctc aggagcagcc taagcagggc agctaatttt
    18241 tcccaacact gagcggcatg ggggaaatgc tgtctctctg accagtcctg agacagtgct
    18301 gtaaatcccc agagctccct ggaatgctgg gtggctagtt tcctgtgatt ccttaccagc
    18361 aagctggaat gtgatggcaa ctatctttct tctttcacaa tactgaacat tattcattcc
    18421 tatgggtgaa agtttgttag tgaagataag ttatctttaa caagatggaa gctgttcctt
    18481 tgagaccata gtccctaccc actctgcata tcactatttc agggcaggca ccacctatag
    18541 agtcaatgtt tttgggatgt ttgatggagg agaaagttta ccactcgttg ggcaagaaat
    18601 gacaactctt tctgacacaa ctgtgacacc atttctatct tctggtaaga aactgagtta
    18661 tatttccatg agaaaatagt attatgaaac tatcgggcaa gtgaagtgtg aggctattaa
    18721 gcaatgaaaa ttataacttt cactacaatt tatttaaata atgacaaact cttttatatt
    18781 tgtagaaact gcttcaaata tccagttgag cctacttttt tcgcttttct tttttttcaa
    18841 tttcgcatta attaaatact ggattattta aaaatgagtt tttattaagt gtaactttct
    18901 attttttctt ttttagtaat gtgattttct tttattaaga tagttatata ttttttactt
    18961 tgagggctta cttggatgga gaaagaaggc tctatacata aagaatcggg caagcatgac
    19021 ttacaagcat gatagtagca cagcccttta gaggttttgt atttgccata attacagctt
    19081 tgatgtaggc gagccagcat ttaatagaat taaaggtgag agtaggagat agagtttcct
    19141 tctaaacttt aagaaaaagt gcttattaaa cccagtaggt cttagcataa tcagagtagg
    19201 ctgtaaagat gtaggttcta gaacagtaca cagcatcttc cattgtgaga aaggctatgc
    19261 acagatggta cttctgtaat tagcagtgtg atgaacattg cttaattttt gttcacttta
    19321 aaagtgatgc aaatatgatc tgataacatt gcagtaaatg agaaagttta taggctgcaa
    19381 gcttctaaaa aaaagaacgt ttacaattaa aggtggataa aagaaagtgt caaaatttga
    19441 tattttgcat tgtttcattg tttctcttat gatgatatta accggcttat atttttattt
    19501 tgtagcttgt cactgaggat attgggatta ttttctcttt ccaaagcccc ttagctttgt
    19561 atctgtgttt gcgttcgttg tctcgacagc tctcaatttg tgtatttctc tgcatccttt
    19621 cccaaattgt cttccttctt cctcaattct ttaaatgtgc aaactttgct gtctgaattt
    19681 acctaatttt tatctgcagt aggtgaaaaa cttgaacagt taataatgag ataatagaat
    19741 agtgtttcga gttttcaaaa cgtgtcttta cttcacaaag gtctgatttt aagactcatg
    19801 tatccagtcc tccataacat gtaatggtta cctggtggct taattattat aaagaatatt
    19861 tttggctcac tggaatcact tacagtttag acatttaacc agtccatctt atgcttatat
    19921 gctaatgtct taaagagtga aggaggatgc tatatcttat agcacatcca catcttattc
    19981 aaattattaa tatttgttgt tagatagcta tgcattctca tttattattt taggtattga
    20041 ttgtgtgagc ctttcaaact aaccatcata gtttaactaa actctctagg gtctatggtt
    20101 gtgatcacaa tatatatata tagtcaccaa tggagaagag gtttgccact gaaatgatat
    20161 ttggagaaag ggattcacat gaatatcatt taaggttaat aaattatagt tgggtcttta
    20221 aattatgaca cctagctgca gtgttaaata gacaacttag gtggcacagg ggacaaccta
    20281 ccaactatgt gcttcttatc gtcctgtctt accgttgcca tgtcagggat ggattgtctc
    20341 accagagctg aagcagacat tgtgttgctg gtggatgggt catggagcat cggccgtgca
    20401 aattttagaa ctgtgcgaag cttcatttct cgtattgtag aagtctttga aattggcccc
    20461 aaaagagtac aaattggtag gtttcctcaa tactgaagac ctttgataac ttacttgcta
    20521 tcttttacac atcataatgt ccatgctgtt atttttagct ctcgctcagt acagtggaga
    20581 ccccagaaca gagtggcagt taaatgctca tagagacaag aagagtttat tacaggctgt
    20641 ggcgaactta ccatacaaag gaggaaatac cctcacaggt gagtcaggat gctttggtga
    20701 ggttttgaca caatgctttt tggtcaggca ggtagctgag aggtgatatg actggctgtc
    20761 ttttaaaaat cccactaggc atggctttga atttcattcg ccaacagagc ttcaagactc
    20821 aagctggcat gaggcctcgt gctcgcaaaa ttggagttct cattaccgat ggaaaatcac
    20881 aagatgatgt ggaggctcca tccaagaaac tcaaggatga aggcgtggag ctgtttgcta
    20941 ttggtaagca ctggagatga tagtgatgcc ctgaggtgac aggtacattg aggacatttt
    21001 ctgtatttaa aaaatattat tggattccca tgcttactga caccaatgaa tgtcagaatt
    21061 cttgaccctc ctaaacaaaa gtctaatgag agagcaacaa aattaattaa caaatgggga
    21121 aaaactaata ctaatcccag ctctagttct tttggaatga acaagactta ttggtatctg
    21181 agaaggagaa ataagaagct taaaaaaaaa agacatccat actttgtctt gatggcatta
    21241 aaaagtgggc tttatttgcc tacaaaaaga agttctggaa ttagggaaag gtaccatata
    21301 tatttagagg aaacatagct ctgttgtgtt ttagggagtt cattacaata aagggatttt
    21361 tatatttaaa actactacag gggagggaga gatggctcag aagttaagca tggtactgct
    21421 cttgcagaat acctaagtcc cattctcaga acctacatca cagccaccag ggtatctaga
    21481 gcctctgtgg gtacctgcac tcatgagatt aacagaccaa tatagacaca gtgatggagc
    21541 actgataaaa agaaaagacc cgaagtattt gcttcatacc attaattttt cctgaaaggt
    21601 gctttcaatt tgtacttaac tcttttttca aggtattaag aatgctgatg aagttgagtt
    21661 aaagatgatt gccactgatc cagatgacac tcacgcatac aatgtggcag attttgagtc
    21721 attgtccaag attgtggatg acctcaccat caacttgtgt aacagtgtca agggccctgg
    21781 tgagcttggt cagatgctgg tgtgtctggg tggggaccct ctcatgtgcc tttagaggct
    21841 tctgcctcct cgataccagg agattcccct gagttgtggt gactaaagat aacagggcaa
    21901 tttttagtat ctgcatctgg tacatggtaa atagttgaag gctttgtgca agttaattaa
    21961 gagacttggt ccttattaca tgtgaaattt tttcttttta tataggaata ttagagtaaa
    22021 acttccctcc gttaatttat tttggttctg ggaaagttag aatgaattta acctcataga
    22081 cacagtagaa atggttccag atgtttgcag aattgccatt actagtgcta gatgtctgct
    22141 ttgaggaact tcctagttaa gggacactgc tttagttcat tttaaaatga ttttttttct
    22201 ttttttggtg gcggggatgg caccaaacta agaaatggct tctagcttat ttaaggattt
    22261 atttaggtta tatggcttag ttgatgcaaa caaaagtggg aagagacaaa ataatggaaa
    22321 gagagttgac agacactcct attctagcac agacctgctt gggggcaaca cagcttcact
    22381 tcagtgctac gttattcaga cagctatgac ttaaaagttg accaatttgg cttgctcagt
    22441 aatgtttggt agcttttttt ttcggcttcc taatgtatag gggaagaca
//