LOCUS       KO-first_condition_ready_24447_MGI:3045251        21691 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Ovch2 targeting project(s): 24447
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Pgk_PM
COMMENT     design_id: 42127
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     exon            8071..8180
                     /db_xref="ENSEMBL:ENSMUSE00000396153"
                     /rank="2"
                     /note="ENSMUSE00000396153"
     exon            4982..5098
                     /db_xref="ENSEMBL:ENSMUSE00000425938"
                     /rank="1"
                     /note="ENSMUSE00000425938"
     misc_feature    4215..9125
                     /note="5 arm"
     misc_feature    9153..9177
                     /note="B1 Gateway"
     misc_feature    9190..9237
                     /note="Frt"
     intron          9238..10112
                     /note="mouse En2 intron"
     misc_feature    10110..10112
                     /note="Splice Acceptor"
     exon            10113..10114
                     /note="mouse En2 exon"
     misc_feature    10326..10887
                     /note="ECMV IRES"
     gene            10898..13944
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13979..14234
                     /note="SV40 polyadenylation site"
     misc_feature    14238..14271
                     /note="loxP"
     promoter        14303..14810
                     /note="mouse phosphoglycerate kinase 1 promoter"
     gene            14897..15688
                     /gene="NeoR*"
                     /note="NeoR*"
     misc_feature    15886..16129
                     /note="SV40 polyadenylation site"
     misc_feature    16146..16193
                     /note="Frt"
     misc_feature    16200..16233
                     /note="loxP"
     misc_feature    10127..10204
                     /note="FCHK std_gpr"
     misc_feature    16249..16269
                     /note="B2 Gateway"
     misc_feature    9126..16299
                     /note="Synthetic Cassette"
     exon            16667..16758
                     /db_xref="ENSEMBL:ENSMUSE00000359375"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000359375"
     misc_feature    16300..16921
                     /note="Critical Region"
     misc_feature    16945..16978
                     /note="Downstream LoxP"
     misc_feature    16922..17001
                     /note="synthetic loxP region"
     exon            21136..21159
                     /db_xref="ENSEMBL:ENSMUSE00000493078"
                     /rank="8"
                     /note="ENSMUSE00000493078"
     exon            19853..20042
                     /db_xref="ENSEMBL:ENSMUSE00000384996"
                     /rank="7"
                     /note="ENSMUSE00000384996"
     exon            19171..19322
                     /db_xref="ENSEMBL:ENSMUSE00000402111"
                     /rank="6"
                     /note="ENSMUSE00000402111"
     exon            18822..18917
                     /db_xref="ENSEMBL:ENSMUSE00000361391"
                     /rank="5"
                     /note="ENSMUSE00000361391"
     exon            18322..18494
                     /db_xref="ENSEMBL:ENSMUSE00000400328"
                     /rank="4"
                     /note="ENSMUSE00000400328"
     misc_feature    17002..21691
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggaaaggga gcctagttaa ggctcaagaa gtaaacaaag atattatgaa
     4261 gtcagtgaaa ctagtaagat tcattaggtc tctctctagc atatgtaagc agtaaagact
     4321 gctgaaatac aatcttggat ccactgagct gtctagaaaa gactcaaacc aacaaaactg
     4381 cctacaaagg acacttcaat ttgttcaact gtctacagac tatattgtgc tctaggtctc
     4441 aatcttttat aatttgtcac ccatgctgga gtaggctttt gttaatgcac tgtctttgag
     4501 tcatttcagc tcctgtgact aacccctcat ccatagtcct gtaagtaatg ccaataaaat
     4561 acattggttc accaagttgg atattggtgg tatccttact ttaatctgtt ctcaattacc
     4621 tatcagggat aagtacacag ttgtttgcat cttgctagaa gtattacaca caaatattta
     4681 atcctcttac agaaaaagga gcaagtaaca atcacaacaa aacctcaaac acaaaccact
     4741 ttgctaagcc aggaacactg gaggaagcaa cttattgatg taggtggaat agaatgttat
     4801 gctgctcagt caagacttca gttttgtagt tgattcccca catctaacaa aaaacatttt
     4861 caagaaacca catgcaaaga catcttatgc ctccagtctc agttaataaa taagcttgtt
     4921 ttaagttgga ttattgctat tcttccaaag cttgctgcct ttgtttcctt cagcctctct
     4981 acgtttcagg aggaagcgtt gaatctcaac atgcctatca gcaaggacaa gctgatttta
     5041 atattaggaa tggtctgctt ggaacaaggt cattctgaaa ccctttcctc cataagaagt
     5101 aagtttcaaa ttaagtgtat aactactttc ggttttgttt ggttggttgg tttgttttgt
     5161 tttttgtctg gtgggatcac tcttgcagca aatcagagta acgtctccac ctcttgttcc
     5221 cttgggaata caagagaagc aaattacttc agaaatgtaa ttggttctca aaaatatttc
     5281 tctggaattt tctcctgtga taagcatatg gaattgaaat aatatgaaat aaatataaac
     5341 agttgtaggg accctaagta ttttctccta gttttaccaa ctgacctagg attggccact
     5401 tatttggaag cattacacaa cctggtattc ttccatggag caaattctgg gagatcagaa
     5461 caggtttgga agtatttaga atcttcaggg aaattatcct aataaccata tgatgcatgc
     5521 tcactgacat atagtataaa tatttttgca ttgattataa tgagcttaaa acagttgttg
     5581 gcaagcttgt ctgtagtcgg acttgtgcca gcccagagaa tcaacatgtt ttctgtctct
     5641 gcttcagtga cttcaggtaa tactaaattt tatgtctagc agatttatga ctcatccatc
     5701 tgaagcagct ggaacagctt ttgatcatac atgcaatggg ctcagaatga aaccacaatt
     5761 ggtcgtttca tgttttgctg ttattagata caggtctcat tgatttttag ttttcacgtc
     5821 ttctacatga agatgaaagt ccttttttca ggcaatgtgt ggggattaag aaataaatta
     5881 tatgggagag ctttatatgc tttaaagacg atctaagtag ctctcctctt aaggatatag
     5941 tcccaagaaa agtcatgtgc tcaatcagcc atgctttcag gtgtaacttg taagccagga
     6001 actgtgctag accttagaag tgtgtatgca taggacccac attcctaagt atctcactga
     6061 agaattctgt agtccaatga gggaaggaga caatttactc tcataacata gcttggaaag
     6121 aactgttaaa ggggaacagg gagtggagag aaagagtgct atattgtggg agagatccag
     6181 gtcccagttt gaagaaatca aggaagactt ttcagatgaa gtggtagtaa gcattcaaag
     6241 agccatctag gtgaagaagc tcagtgataa tgatggagag ttttatatgc actcttggaa
     6301 acttcatagg aaaaaataac ctcaagagaa acaaattcaa cacagaattg cacagtgtaa
     6361 ttaaacaaat ttaaactcac cccttactta agaagtcaaa agcccacata aatgcacaca
     6421 tattgaagtg ttgtcgggga tgaaggcttt cttgttaagt tatgtcttct ctttacctga
     6481 caggcacctg tcttaacacc agtgtgtgaa ttaaaactat catcatacca ctgttcctga
     6541 acaatgtatt ttttgcccta tagtaaatct aattaaattt cttcatatct aactgtgtgg
     6601 caggcatgca ttaaagactc agtctcatag gcagagcaga gagagagaga gatagacaca
     6661 attaaaaact aggacagact acagcatcat ccataagtta actaagcaac tctgcatatg
     6721 tgaatttgta attccattgt gcctatgcta gatgtgatgt tacacacctg taatccaaaa
     6781 gcagacttcc aaaagcagag gcagggaaaa catgaactta aggtcatcct gagctccata
     6841 ttgaaatgaa attctctcac aaagaaaagg aaggaaggaa gaaaggaagg gagggaggga
     6901 gggagggagg gagggaggga gggagggagg gagggaggaa ggtatcccca ttgttgtgga
     6961 gtccacagtc tacctgggtt gaaagccaga aacaatcatc ttggtacaca gtgagtgtga
     7021 gtgtcgcagg atactacaaa gtacctctct ttttttgaga ccagagagct ctgaaagaaa
     7081 agagactgaa ttcctgacaa cccagtgcca attatccctg cagcagttaa ccttcacatt
     7141 agaagtcagt cctttgatgt tatgccagtg cctggcaaat acagaagtgg atgctcagtc
     7201 tggatggaac acagggcccc caatgaagga gctagagaaa gtacccaagg agctaaaggg
     7261 gtctacaacc ctataggaag aacaacaata tgaactaacc agtaccccca gagcttgtgt
     7321 ctctagttgc atatgtagca gaggatggcc tagtcagcca tcaatgggag gagaggccct
     7381 tggtcttgca aagatcatat gcccagtaca ggggaatgcc agggcaaaga agcaggagtg
     7441 ggtgggttgg ggagcatggc gggggagggt ataggggact ttaaggatag tatttgaaat
     7501 gcaaatgaag aaaatatcta ataaaaaaat agaagtcagt ccttggtctg ggtaaaaatg
     7561 gttcagtcag cactgctttc tatgcaagca caaagaactg ggtttaatcc ccagaaacca
     7621 ttttttaagg tgatacagta atgtgcattc ccaaatgcac ttgttgagag tcagtgatgg
     7681 gcagatcctt ggggctagct ggagagttgc aagctagtga gagatcttgt ctctaaaaac
     7741 aatgggctaa ggaagatagt caaagttgac ctctcgcctt aatatgccca cacatggata
     7801 aacacacaca cacacacaca cacacacaca cacacacaca cacacacacg caccatagaa
     7861 gctctctctc tcaacagcac acatcttatg tgtgacaaaa ctgagtaatc tgttcttcaa
     7921 aacacatcta atcccatcca ttaacttaaa gccgtattgt aatttcttta catatcccat
     7981 atactgcatt ttgttcttgt ttggaaaatt cttcactata tcttagtcct agaaattaat
     8041 cactcagtgt ctatcttgtc tttttcccag atcctgactg tgggcagagt ttggttaagc
     8101 cccaacctca gaattacttt agccttttca gtcgcattgt tggaggaagc caggtggaaa
     8161 agggttccta tccttggcaa gtgagtcttg caattccttc tctagttggt gaaataggat
     8221 atttcaagct tgtgtcatcc agatcacatg ctacttccct tagatcaccc tctgtctagg
     8281 aaaaaaaaat tcacaacaca cccagagcta tgctttccta atcttaaaag aagattaatt
     8341 aatattcatg attgaatttg tgctcaacag gttgcctgtg tgttcttgat aaacataaca
     8401 aatatttttt aagtagatga ctggtcactt gaccaattaa ttggtcatct gttcaattca
     8461 gaagttcata ttcaaatcat aataatggtg aaccattggc ttttctttca tgattttctg
     8521 ttctgtcttc tatatttagc ctcctctcag tattcctcct gtttgtttat tcagtatttg
     8581 ttttggttca aattatttaa tctagagtat aaaaagctct aagcattcac tgtaccattg
     8641 aactttatat aatcttgcca gattttctgt gttgctttgt gtttcatgtt ttctttttga
     8701 ttgttcataa ataattttta cttaaaagtg aaaaattctc agctggacag aggccccagg
     8761 cacatattta gcagaggact ccctttcctg acctcagtgg gagagaatgc acccaatcct
     8821 gtagagattt catactccag ggaaggggga caccaggcat gggagaaccc tcttagagtc
     8881 aaaggggaag aagatggggt aaagaacttt gggaaggtat gcctggaggg gggatcaaca
     8941 tttgagatat aaataaagta attcgttttt aaaaggtgaa attttctaat aaactaccat
     9001 tttctcatcc taaaaatgtg aagctattca ttgaaagtaa ttatttgtca agtataagtc
     9061 acctttgaat caaatcaaat atcatagatg gattgagtta aagacagaaa attactaaag
     9121 atcagaaggc gcataacgat accacgatat caacaagttt gtacaaaaaa gcaggctggc
     9181 gccggaaccg aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcgaa
     9241 ccctttccca caccaccctc cacacttgcc ccaaacactg ccaactatgt aggaggaagg
     9301 ggttgggact aacagaagaa cccgttgtgg ggaagctgtt gggagggtca ctttatgttc
     9361 ttgcccaagg tcagttgggt ggcctgcttc tgatgaggtg gtcccaaggt ctggggtaga
     9421 aggtgagagg gacaggccac caaggtcagc cccccccccc tatcccatag gagccaggtc
     9481 cctctcctgg acaggaagac tgaaggggag atgccagaga ctcagtgaag cctggggtac
     9541 cctattggag tccttcaagg aaacaaactt ggcctcacca ggcctcagcc ttggctcctc
     9601 ctgggaactc tactgccctt gggatcccct tgtagttgtg ggttacatag gaagggggac
     9661 gggattcccc ttgactggct agcctactct tttcttcagt cttctccatc tcctctcacc
     9721 tgtctctcga ccctttccct aggatagact tggaaaaaga taaggggaga aaacaaatgc
     9781 aaacgaggcc agaaagattt tggctgggca ttccttccgc tagcttttat tgggatcccc
     9841 tagtttgtga taggcctttt agctacatct gccaatccat ctcattttca cacacacaca
     9901 caccactttc cttctggtca gtgggcacat gtccagcctc aagtttatat caccaccccc
     9961 aatgcccaac acttgtatgg ccttgggcgg gtcatccccc cccccacccc cagtatctgc
    10021 aacctcaagc tagcttgggt gcgttggttg tggataagta gctagactcc agcaaccagt
    10081 aacctctgcc ctttctcctc catgacaacc aggtcccagg tcccgaaaac caaagaagaa
    10141 gaaccctaac aaagaggaca agcggcctcg cacagccttc actgctgagc agctccagag
    10201 gctcaaggct gagtttcaga ccaacaggta cctgacagag cagcggcgcc agagtctggc
    10261 acaggagctc ggtacccgga agatctggac tctagagaat tccgcccctc tccctccccc
    10321 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
    10381 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
    10441 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
    10501 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
    10561 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
    10621 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
    10681 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
    10741 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
    10801 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
    10861 tgaaaaacac gatgataagc ttgccacaac catggaagat cccgtcgttt tacaacgtcg
    10921 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
    10981 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
    11041 gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct
    11101 ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg
    11161 ttacgatgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc cgccgtttgt
    11221 tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct
    11281 acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg
    11341 caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag
    11401 cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg
    11461 cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt
    11521 gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga
    11581 tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct
    11641 acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt
    11701 cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa
    11761 cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga
    11821 actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga
    11881 ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt
    11941 taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca
    12001 ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa
    12061 ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa
    12121 tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc
    12181 ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat
    12241 catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg
    12301 gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac
    12361 ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc
    12421 tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat
    12481 cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca
    12541 ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc
    12601 gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga
    12661 tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca
    12721 tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca
    12781 aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tcctgcactg
    12841 gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc
    12901 acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact
    12961 ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat
    13021 cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc
    13081 ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa
    13141 gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa
    13201 acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg
    13261 cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg
    13321 ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt
    13381 gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa
    13441 aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag
    13501 cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg
    13561 ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg
    13621 ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga
    13681 aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga
    13741 cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca
    13801 tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg
    13861 tggcgacgac tcctggagcc cgtcagtatc ggcggaattc cagctgagcg ccggtcgcta
    13921 ccattaccag ttggtctggt gtcaaaaata ataataaccg ggcagggggg atctaagctc
    13981 tagataagta atgatcataa tcagccatat cacatctgta gaggttttac ttgctttaaa
    14041 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    14101 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    14161 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    14221 tcatgtctgg atccggaata acttcgtata gcatacatta tacgaagtta tgtttctagc
    14281 ttggctgcag gtcgtcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg
    14341 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
    14401 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
    14461 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
    14521 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
    14581 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
    14641 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
    14701 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
    14761 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcctgt
    14821 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
    14881 aaccatggga tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
    14941 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
    15001 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
    15061 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
    15121 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
    15181 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
    15241 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
    15301 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
    15361 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
    15421 gcgcatgccc gacggcgatg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
    15481 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
    15541 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
    15601 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
    15661 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
    15721 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
    15781 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    15841 ctggagttct tcgcccaccc cccggatcta agctctagat aagtaatgat cataatcagc
    15901 catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    15961 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    16021 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    16081 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ggggtaccgc
    16141 gtcgagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcgtcgaga
    16201 taacttcgta tagcatacat tatacgaagt tatgtcgaga tatctagacc cagctttctt
    16261 gtacaaagtg gttgatatct ctatagtcgc agtaggcgga atacacccag attatcctga
    16321 ttgctacatg catattatgt acactttgta cataaacaca cacacacatg catatacatg
    16381 cacacacact caaagaggag agagtgatgg ataaataaat agatagatgt cattttaagt
    16441 atgttgaaga aacaaattaa agcacaggct tgaagttgta ggggtcaaat agtctattaa
    16501 gaccatatga aaacagtagg cccaaactac tcttgttcac actgtcgtga tgcctgagtc
    16561 atgatcactg tggttatctc tatctgtctt tcagaatatg ctcagtcata ggtttgggga
    16621 aaccccacag cattggcttc caccaactta gatctctgtc atctaggtgt ctctgaaaca
    16681 aaagcagaag cacatctgtg gagggaccat catctcttca cagtgggtca tcacagccgc
    16741 tcattgcatg gctaacaggt aggaaacctt ggttgatgga ggaagacaag gttaccccat
    16801 cctgcacctt ggaatcagaa ctgattactc ttgtaaaaca caaggaagat aattgggtgt
    16861 cacaaatgag gcttagtgac attgtaggct aattctcagg ttagtaggta aaggattctg
    16921 tgagatggcg caacgcaatt aatgataact tcgtatagca tacattatac gaagttatgg
    16981 tctgagctcg ccatcagttc ataatagttt tgattaaaat gttgtcgtgg aaaaacacta
    17041 cctggttcat tatacatgtt aagaaaacaa agacagacca ataaaataca gatgtttatg
    17101 tcttccttcc tgttggactt gcttctctga gcttcaacct aagcactttt atttttttaa
    17161 ttagctttct agtgtgatcc aaatactcca ctgttggcat tctatacata tactaggctg
    17221 tagtcaagat tatgtcccca tggttgttct ttcatttcaa gtctaaaagc attcttggga
    17281 tgctcattaa ctgccagttg ccaatgggta tcaaatacac aaaaattact aggcatgatg
    17341 cccccagtgg gtccatagtt taaaagagaa attagactgg aatggtgtgg tacatcttta
    17401 gacacagtta cttgagcagc tgaagcagta aaattgcatg agtctaggaa tttggggcca
    17461 gcctgaggga tatagaaaga ccacctatgt tgagtaaaaa taaaaataaa caagcaaaca
    17521 aacaaacatt taacaatgtc cctgcaatgg aggcagtggt atgctagaga gatgctatgt
    17581 attatagtaa gtacctgata tatccaagat tcgggaaaac attacaatag aaatgatggt
    17641 gctctatcta atattatata aaatatcacc attggaaaag aagggttcca aaggaagaaa
    17701 agctcaaaat gcttacttca ttccatctca tcctatcaag ttccttcaag ataacactga
    17761 ccttaagaaa tggcatcatt ctcattttgt gggagaacag taaattacaa atataaagaa
    17821 ggcagaggca atccctgctt cgggctgttg taattggtcc aaatccttga gtatgtgtac
    17881 tctctggaac atcagagtta ggttgctgtc cttcataata cacacaccac gtgagatttt
    17941 taacactgaa gcaacaccct tactaggaat cacaaatact tcacaaaata actcactggt
    18001 gttatgacta ggacagccta ataagatagt tgagtggttt catatatatt ttagctccct
    18061 ggattacaat gcatgtctgt ttctctagct gattataaac aacactggca aatattaaat
    18121 atatcatgtc actatctctg cgctgggtct tctgtttttt ttttttaaat agcagtagaa
    18181 ccattctcgt tcgcttggct cagcactccc atgctccaca ttatccaccc atgttaattt
    18241 gaagaggcca attttccagc atttacaacc atacttagaa gctcattaaa accccatctc
    18301 tttccttctc cctttttata gaaacattgc attaactttg aatgtaactg ctggagaaca
    18361 tgacttgagc caagccgagc caggagagca aacacttgcc attgaaacca tcatcataca
    18421 cccgcagttc tccaccagaa aacctatgat ctatgatata gcccttctga agatggctgg
    18481 aaccttccaa tttggtaaac atttacagtg gaccctgaat aatctatacc ataacctttg
    18541 atccatacta tacctctaca gatcatatta tgtagcatga gaccagccct tctggattat
    18601 atacgagaag tggagacttg tccggagtgt ccccttttta aaatgccttc cttatggttc
    18661 tggatgtagg gttatgtaga ctgaggagaa ggaaactgac aagtgacccc tgaagatgcc
    18721 aggctgatcc ttaagaggtc tttggagact acccacatgc caaaaggtgt aactgtgtta
    18781 acttctccat aagccaaatc ctgccttgat cttttcttca ggacagtttg tgaggcctgt
    18841 gtgtcttcca gagccaggag aacactttaa tgctggattt atttgtacaa ctgcaggctg
    18901 gggtcgcttg tccgaaggta agagctcctg taccacttct cttaatcaaa atcctcaaaa
    18961 cgttctgaca gataacgaga aaccagaatt tttaattaat taaacaacca ggcaaacatt
    19021 tcaaaagtca gagaacttca caatgcgctt aggaaattga gatccttcca aagagacaca
    19081 aacttgcatc cagctggtgc tgatgaaagt acagggcgtc ttcatctcag aaggtaaaag
    19141 cttacctgat gggtcatctc ttcattccag gtggcaggct cccacaagtc ttgcagcagg
    19201 taaacctgcc aattttgact caggaggaat gtgaggccgt tctgttgacc ttaaagaatc
    19261 ccattactgg gaagaccttt ctctgcacag gatcccctga tggagggaga gatgcttgtc
    19321 aggtacgtgg gtaagctaag ctccaagcac aggaaagttt gctagcaggt gaggagtcta
    19381 agaacgctga taccaggtaa aactagaatc tgttccccac caccacccca aaaaaataga
    19441 gatagtgcca caggtgaagc cctcaaggtt ctccactcat tatggatatc tcttcttatt
    19501 ttgaggttcc tagattagag gtgttggaaa tgtcactatc ctcaattctc aggatttttg
    19561 aagacttcag tcagttgtat cactgggcaa gaagtcactg attctgtagt atgaagaagg
    19621 atgggaaaca ttgatgaaca ctaagaatgg ggagaaggag cagaagttga cagattctgt
    19681 ttgaggatca aatggaaatt gtaaccagat attctcccag acccctgagt cttaattgtt
    19741 agaagtgaga attgttagtg cttgacattt ccatcctcgt ggggaaagtg agcactctga
    19801 ggcacctcca ccaacaatca cagtaacagg aacttccttc ttttttccac agggagattc
    19861 aggagggtca cttatgtgcc agaataggaa aggggcctgg actctggctg gagtgacttc
    19921 ttggggtttg ggctgtggtc gaagctggag aaacaatgca agaaagaaag agcaaggatc
    19981 tcctggaatc ttcacagatc ttaggagagt gcttccctgg atcctcaaac atatccagac
    20041 tggtaatgaa gccatcctgc aagcttagac agctacagtt ctaccggggt aaagagcatt
    20101 agcagactag acaatcgtaa ctggagaggg ccacttcttt tctcagtgta atgcttgagg
    20161 attttgaaat gtatgatcta ctacaagagc aaaacatcat ataaatctaa gtattaaaat
    20221 atttactgaa gaatgaatgt attcctcata aattgattaa actttgccta tgatagaaac
    20281 tatgtcatcc actaatgtag atagataggt agatagatag atagatagat agatagatat
    20341 ccacctacta tcctagcaag attcagcaag cgaaagcgca gctcagtaat gaacaaccac
    20401 tgataatctg actacccaga gaatgatatt tctctggaaa tattttcaga tgtcacaatt
    20461 aaaaagggga caccagcacc tagtagatag aggtcacagg gccttctaag ccatgtacag
    20521 tactgcaaac agtgctcacc gtaatgaata gtgtcaccta aataaataag acaggagatt
    20581 tagaaatcca ggtataaaag caaatgtggt aaatttgagg gacaggctct atgtgcaccc
    20641 ttctagtaga ccagccagga ggaaaaaaaa tgcagcttcg tttctacctc caaactgttt
    20701 ccttaactgg tgtcttcaag tctcaaagtg gcttccatca tttctcagtg gattaactta
    20761 agttacctca gaattttttt caagagtata gtataatgac aggtatctga acgtcagtgt
    20821 gggactactc aaaatgttca tgattggagg caggttatct ctatctctgg cctccactct
    20881 caagcatcct tggctccttt tgttctgtgt aatctccaag ttaatcactg tttcgtcgtt
    20941 agtaagaata aagcacgtga gatcatctca aaactccagg accgctatcc tgctttatca
    21001 ttttacctgg cctgttcttt tcacagatta aattttcagt atttacatgt tgacttaaaa
    21061 tgctctgctt ttcaagtcaa tttcttgtcc aaggtaccac aagctttgaa ttgcatcttt
    21121 ctttttcttt ctcaggccat cgaaggaaga gcaccaaagg tgggtattcc ttgtagcctg
    21181 cactcgcagg agcatgagtt aacatcctaa catttctggg agtcagttcc tgttatcatc
    21241 taaaatcttc ttctggttgt gtttcagtca tcacttggta taaaactctg atgagcatcc
    21301 acagctgtcc ctgccagtca gctaattaaa tgtccacgga gccccattcc cttacatata
    21361 tctttgtatc aagtcatagc tttatccaat tcagcaaaca ctaagtgaca aacactgact
    21421 ctcaattaaa taatagaata ggggaagtgg tacagaaata gttaagtgac atacactccc
    21481 ctgaaagttc ccctacttac acagggggct tatccctgtt caccatgact ctttgatgaa
    21541 ttcactctga aatttggggc aagccctgtc ctcggttaca ttatctatag caaaatgaga
    21601 gtggatcttg tcttagttgt ctactgggcc tttgagccta taggattatt cctaaaaaag
    21661 caaaatgaaa aatagactcg ctagatctag g
//