LOCUS       KO-first_condition_ready_31532_MGI:2681844        21871 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Acsm4 targeting project(s): 31532
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Pgk_PM
COMMENT     design_id: 42446
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            4438..4681
                     /db_xref="ENSEMBL:ENSMUSE00000421258"
                     /rank="2"
                     /note="ENSMUSE00000421258"
     exon            5441..5651
                     /db_xref="ENSEMBL:ENSMUSE00000233168"
                     /rank="3"
                     /note="ENSMUSE00000233168"
     misc_feature    4275..8924
                     /note="5 arm"
     misc_feature    8952..8976
                     /note="B1 Gateway"
     misc_feature    8989..9036
                     /note="Frt"
     intron          9037..9911
                     /note="mouse En2 intron"
     misc_feature    9909..9911
                     /note="Splice Acceptor"
     exon            9912..9913
                     /note="mouse En2 exon"
     misc_feature    10125..10686
                     /note="ECMV IRES"
     gene            10697..13743
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13778..14033
                     /note="SV40 polyadenylation site"
     misc_feature    14037..14070
                     /note="loxP"
     promoter        14102..14609
                     /note="mouse phosphoglycerate kinase 1 promoter"
     gene            14696..15487
                     /gene="NeoR*"
                     /note="NeoR*"
     misc_feature    15685..15928
                     /note="SV40 polyadenylation site"
     misc_feature    15945..15992
                     /note="Frt"
     misc_feature    15999..16032
                     /note="loxP"
     misc_feature    9926..10003
                     /note="FCHK std_gpr"
     misc_feature    16048..16068
                     /note="B2 Gateway"
     misc_feature    8925..16098
                     /note="Synthetic Cassette"
     exon            16468..16675
                     /db_xref="ENSEMBL:ENSMUSE00000400031"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000400031"
     misc_feature    16099..16829
                     /note="Critical Region"
     misc_feature    16853..16886
                     /note="Downstream LoxP"
     misc_feature    16830..16909
                     /note="synthetic loxP region"
     exon            21159..21302
                     /db_xref="ENSEMBL:ENSMUSE00000412236"
                     /rank="5"
                     /note="ENSMUSE00000412236"
     misc_feature    16910..21871
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaattgtt agggctaagc tggtgacatg cagaattaag aaacattcag
     4321 tagtggggac actgatcagt ggctaatggt gacagaccta tgcgaggatg gagcgttctt
     4381 caggaatcca ctcccagtct tcagctaaca ctctcccctc ctctctcttc cctacaggca
     4441 tttcccactt gctaccagaa aataaagttt ttcaggaacc atgaaggttc tcctccactg
     4501 tcagagactt agattcatct ggcttgccaa gccagccggc cggcacttcc acagagattc
     4561 ccagctctgg gcacctctga cactggatga ctttgaagcc atcaatcgct gtgagaagcc
     4621 attacccaag aactttaact ttgcagcaga tgttctggac cagtggtccc taaaggagaa
     4681 ggtatcaaag agcagggccc cagtgttcct gttgctctca tctctgtctt ccaggtcctc
     4741 tcactgtgtt ctacctgtca agtttaaatc aattttttta tctcagtgag ggcaggaacc
     4801 tggatggctt catggagact cataagtaca agagcccata aacacctttc attccaatca
     4861 gtttctgtct tttgattctt ctcagaaaac ttagagtcta gggagatgaa ttcagattaa
     4921 ctcttccttg gtatatgctt atcccctctt taaaaagggc agaccccacc aagaggtgat
     4981 tctccaagac tgttctccag tgttccactg gagagaaaca tttctcccct aggcacactg
     5041 gggaagcagc tctggaggag tagcaataga cgtcctatct gccttctgtg ttagtcagtg
     5101 tgccatcact gtgacaaaac acctaagaca cacaatttca agtgaatgaa ggttgcttcg
     5161 gttcatgggt gcaacgatat cagcttcggt tcacttagcc ctgtggcagc cctgagcagc
     5221 acagggaaca tgcatggtga ggaagactgc ctgctcccct ccaggacagc tcagagaggt
     5281 ggaaggaatg agtgagagga agggcctagg gtctgatcgt tttcttcttt aagccctaac
     5341 gccacaggat aggggactat gccttttaac atgtgggtct tcaggtgaca gctctaaacc
     5401 ataatacatt atcttctctt tcccatgtgt tcctggccag tctggagaga ggccagccaa
     5461 cccagccctg tggtgggtga atgggaaagg agatgaggtg aagtggagct tccaagaact
     5521 gggttccttg tcccgaaagg ctgccaatgt gctcaccaag ccctgcggcc tgcagagagg
     5581 agaccgggtg gctgtgattc tgccccgtat ccctgaatgg tggctcatca atgtggcttg
     5641 catgagaaca ggtgagtatc acaaatgagt gtcaaaactt gagacttttg tgaagggtca
     5701 gctttgacta aagcaatcct tacaaaaagt agagaaagaa agttaacctt aatagcagtt
     5761 gtgatgaaat aataacaaca aacttaagaa attgggaaca ctgtgataac cattttttga
     5821 gagaggaaat caaagcacag tgatattaag acatttgcca aaggtcacat cacttgaaaa
     5881 ttatgtaatt ggggacctgg gaagatgact cagtggataa agtacttgct gtacaaacat
     5941 gtggccctgc attcccacat caaaagcttg gcatggaggg acaggtttct atgatcccag
     6001 cactgtgggc gcagagacag gcacatcccc tggtctcact ggccagccac acctagtcaa
     6061 gacagaaagt ctcaggttca cacacatata cccacacaca caaataaaaa tgaaaaagag
     6121 agttgcacta ttcagagttg cagaaaaaag ctctataaag caaatgcaag tgtgagtgtt
     6181 tctccacagc acagggtgtg tggaatatcc cccccataca cacacacaca cacacacaca
     6241 cacacacaca cacacacaca cgcgcgcaca cacgcgcgca cacacacaca cacgcacaca
     6301 cacggttctc tcttctgggt gtgtatctag attctctgca acacaaaaat acagaagaag
     6361 tggaagtctt tgaggatgca aagatttgta agaacataag ggactctgga cccaagtttg
     6421 gaaaaaaaaa aaaaactcca gtcagaagaa ctaatgtatc cagttacaca ttttccccaa
     6481 agtagtaaca tgtaaagtaa agacatgtct ttcagatagg ggtctgtgag ccatgaacta
     6541 tggttcctct gcttgggtaa atagacgtgt gatggaatac agctgactcc ttttgctcca
     6601 cagcaaacgt gctgactgag accaagaaca atcctacagg ccacagagtc taacacactc
     6661 tccaggcctt ttaccaaaag actctctgat ttcttatctt taaaaaaaaa aaatcatgcc
     6721 gggcatggtg gcacatgcct ttaattccag cactcaggag gcagaggcag tcagacttct
     6781 gagttcaagg ccagcctggt ctacaaagtg agttctagga cagccagggc tatacagaga
     6841 aaccctgcct cgaaaaacca acaaaaaatt aaaaaataaa aaaaatcatc agacatccat
     6901 taaaataaat aagaatgcca aagtgtagac tactacagag aacagaaaga tatctgtgca
     6961 atgttattag caggtataca aaaccaaact taaatagaaa tgattaaaca atgcataacc
     7021 tagctaaggt gtaagtttag ttgagttgtt gattgatggt tgaatggctt gatcttgatt
     7081 aaaaaataat ttatactagc aataataaca tcaggagaat ttcagagaat ttaagatgct
     7141 tttaatgcca ataaaataca tgaaagcagg agtataaaaa tgtttaaatt caccatataa
     7201 aaagacagag ataaattgga aatcagccgt ggttggaaga agattaagga tggtttatct
     7261 gttatgaaat atacccttaa tgtaatttaa agtaaattat ttaacttagg aaaatctgtg
     7321 agtacaaaca cacaagctag tgttcaaaaa catgaaataa aacaataaca acagttcatc
     7381 aagtgaagaa agccagctgc agacctgctt attccccaaa atgtcaaacc cttcatgtga
     7441 atttttaaaa ttgccttcac tcaccacata acaaactatt tgattttgct ggaaggcata
     7501 tgcaataaag tctaaaatac ttgccatcca acaaacattt gctgtctaaa acccattaga
     7561 agaaatcttc ctctgactcc caaggtggta atctactact gaacacattc ataaatccac
     7621 cacggagact gtaactggga atcattaaga gcaaataact tgtaagagaa atcattttat
     7681 atttttatat taattgatcg ttctgagact ctctaacgtg ttttcttaag ggaataactc
     7741 agctaactca gttaaaatat actcagtaaa atagccttgg gatggcaaga tggctctgca
     7801 ggtaaaggtg cttgctatgc aagtctggcc accagagctt ctgagtccat gagaccaacc
     7861 taaaggtgga aggagagcgt tgatattaca aaaggtgtcc tctgcccata tgcgtaccac
     7921 agcatgagag ctccccacaa taacactgat gacagtaaaa aaagagcctc cttcctagga
     7981 attcaactcc acaacttcct cctatttagc tgatttcaga atacccagca aggagtacat
     8041 acatagacat acaacacagg tcttgggagt gatagaaatg tctctgggga ctaaccttgc
     8101 tattggcatt gccatttatt ggctttgtga cccgtaggtg cgttatcatt cactggcatg
     8161 tgatatgtgc tctgtgcacg gcagtggcta tctgtaatgt tttgagtatc actgtcttcc
     8221 catggattca ctttagagag tcaaaccacc tgcttacatc ttatccttac atctaatcct
     8281 tacaccatac atgtaatcct gccctgtgca gtcttctcat aaggagcttt gaaatcctgt
     8341 ctctggctgc tgtttgggat gaagagggat aaattaagca tgaccatcgt catctaggaa
     8401 aatcttggcg gtaccagacc acggcatcaa tgatttgtag gctggctctg ccactcacca
     8461 gctatcccca aagactgcat gcacctcgtg gggatgctta aaacagtagt ttcaggttac
     8521 taaggtggct tggttactaa agatgcttgc tgcacgagcc tagctacatg agttagattc
     8581 ctaggaccca caaaaaggta caaggaaaga accacatcca caatggtatc atctgacctc
     8641 catgcaaaca acacacacac atacacacac acacacacac aaatgataga ttaaaaaaaa
     8701 tccttttaag tttttgaaga atagtctctg agcatctggg gctatgcaat ctatcaaaaa
     8761 aaattaatga gcaggcattt tcctagctgc atataatttt tcactctgtc ctgttgcact
     8821 gatgtagtat gcatcttaca aagcatttca acaaagagaa gaattctagt gtctctgtac
     8881 cttttacata tctattattt gcttttctca ttcccagata aattaaggcg cataacgata
     8941 ccacgatatc aacaagtttg tacaaaaaag caggctggcg ccggaaccga agttcctatt
     9001 ccgaagttcc tattctctag aaagtatagg aacttcgaac cctttcccac accaccctcc
     9061 acacttgccc caaacactgc caactatgta ggaggaaggg gttgggacta acagaagaac
     9121 ccgttgtggg gaagctgttg ggagggtcac tttatgttct tgcccaaggt cagttgggtg
     9181 gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa ggtgagaggg acaggccacc
     9241 aaggtcagcc ccccccccct atcccatagg agccaggtcc ctctcctgga caggaagact
     9301 gaaggggaga tgccagagac tcagtgaagc ctggggtacc ctattggagt ccttcaagga
     9361 aacaaacttg gcctcaccag gcctcagcct tggctcctcc tgggaactct actgcccttg
     9421 ggatcccctt gtagttgtgg gttacatagg aagggggacg ggattcccct tgactggcta
     9481 gcctactctt ttcttcagtc ttctccatct cctctcacct gtctctcgac cctttcccta
     9541 ggatagactt ggaaaaagat aaggggagaa aacaaatgca aacgaggcca gaaagatttt
     9601 ggctgggcat tccttccgct agcttttatt gggatcccct agtttgtgat aggcctttta
     9661 gctacatctg ccaatccatc tcattttcac acacacacac accactttcc ttctggtcag
     9721 tgggcacatg tccagcctca agtttatatc accaccccca atgcccaaca cttgtatggc
     9781 cttgggcggg tcatcccccc ccccaccccc agtatctgca acctcaagct agcttgggtg
     9841 cgttggttgt ggataagtag ctagactcca gcaaccagta acctctgccc tttctcctcc
     9901 atgacaacca ggtcccaggt cccgaaaacc aaagaagaag aaccctaaca aagaggacaa
     9961 gcggcctcgc acagccttca ctgctgagca gctccagagg ctcaaggctg agtttcagac
    10021 caacaggtac ctgacagagc agcggcgcca gagtctggca caggagctcg gtacccggaa
    10081 gatctggact ctagagaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
    10141 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
    10201 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
    10261 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
    10321 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
    10381 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
    10441 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
    10501 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
    10561 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
    10621 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
    10681 tgccacaacc atggaagatc ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
    10741 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
    10801 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
    10861 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
    10921 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac
    10981 caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg
    11041 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat
    11101 tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta
    11161 cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga
    11221 aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg aagatcagga
    11281 tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca
    11341 aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga
    11401 ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg
    11461 gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga
    11521 gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg
    11581 gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac
    11641 gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct
    11701 gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc
    11761 tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca
    11821 gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct
    11881 gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt
    11941 gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac
    12001 gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga
    12061 atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc
    12121 ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc
    12181 gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa
    12241 aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc
    12301 gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg
    12361 tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa
    12421 cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt
    12481 ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa
    12541 acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga
    12601 atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa
    12661 gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga
    12721 actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt
    12781 gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg
    12841 tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac
    12901 caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca
    12961 gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg
    13021 cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat
    13081 tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc
    13141 gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc
    13201 gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag
    13261 tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg
    13321 gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg
    13381 gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc
    13441 attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac
    13501 gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg
    13561 ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg
    13621 cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc
    13681 gtcagtatcg gcggaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg
    13741 tcaaaaataa taataaccgg gcagggggga tctaagctct agataagtaa tgatcataat
    13801 cagccatatc acatctgtag aggttttact tgctttaaaa aacctcccac acctccccct
    13861 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    13921 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    13981 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccggaataa
    14041 cttcgtatag catacattat acgaagttat gtttctagct tggctgcagg tcgtcgaaat
    14101 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
    14161 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
    14221 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
    14281 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
    14341 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
    14401 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
    14461 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
    14521 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
    14581 cctcttcctc atctccgggc ctttcgacct gcagcctgtt gacaattaat catcggcata
    14641 gtatatcggc atagtataat acgacaaggt gaggaactaa accatgggat cggccattga
    14701 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
    14761 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
    14821 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
    14881 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
    14941 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
    15001 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
    15061 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
    15121 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
    15181 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgatga
    15241 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
    15301 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
    15361 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
    15421 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
    15481 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
    15541 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    15601 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    15661 ccggatctaa gctctagata agtaatgatc ataatcagcc atatcacatc tgtagaggtt
    15721 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    15781 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    15841 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    15901 atcaatgtat cttatcatgt ctggatccgg gggtaccgcg tcgagaagtt cctattccga
    15961 agttcctatt ctctagaaag tataggaact tcgtcgagat aacttcgtat agcatacatt
    16021 atacgaagtt atgtcgagat atctagaccc agctttcttg tacaaagtgg ttgatatctc
    16081 tatagtcgca gtaggcggat taaatatcac acaatcatta cacaatcacc tttcaagcct
    16141 gacatgaaag tggttcttac tactcagccc atcattgtca accagctgct gtcattatcc
    16201 ccatgataaa agtaaagaag ggaaggccag agaaacccaa gtcatagggc tagcaagttg
    16261 caagaccaag actagaccca aggcctgtca gattccaaag ccagagctct actgatccct
    16321 catgccaggg cccagcagga cagcccaggg aaccaagtta caggtagctg agggctgaca
    16381 tctgaagttc tcattcatcc agacacaaga cctagatttc ccaataatct ccagagctgt
    16441 tagctcagga atcacttttt gttccagggc ttgtattcat gccaggaacg atccaactga
    16501 ccagaaaaga catcctctac cggctgcaag catccaaggc caagtgcatt gtggctagtg
    16561 aggaagtggc ccccgcagtg gactccattg tatcagagtg tcccagcctg aagactaaac
    16621 tcctggtgtc tccacaccac tgggatggat ggctcaactt tcaggagtta cttcagtgag
    16681 tatttgggtg ggaggcacta gcaacagcac aaatcacagg tacagctctg aatctgtccg
    16741 tccaaagact cagcaatagc cagagttgat accgggctcc ttcccaaaac agaatgtttt
    16801 taagagtatg agcttatgag tacatgtgtg agatggcgca acgcaattaa tgataacttc
    16861 gtatagcata cattatacga agttatggtc tgagctcgcc atcagttcag aacatatgtc
    16921 ctctgtaata ataataggta ctcttagacc ctagaccatc tctccagctc cctaaaaagt
    16981 catttaatga aattatttac agacagaaga aatatgtaca gctctactct gacttttctg
    17041 tctcctctgt ggcctatgat gagtgtgcta tagagatggg cctttgggac acaactatca
    17101 tgatctctct tttactcttt gaacatgctc tagctcagaa ccttggcatc cactgtctct
    17161 cctgccccga tgttctttcc tagtcctttc tatagttgtt ctatcttgtc aggtttcagt
    17221 tcaaatgttt tatcctacgt gatgctgctc ctgaccatct tgatggaagc cgtaaggctt
    17281 ttttctccat gcatggactg tttcttcgcg ttgctaggtc ctctgagtga tcagactagc
    17341 ctcgtgttct tttagattca gtttttctac tccaaggttt ggactggaaa attaggctcc
    17401 aaagccaaat cgggtccacc acttgctttt gtaaataaag tgtcattggc acacagaggt
    17461 gctcatttgt ttgtatattg tctatgtctg ctttcattta gaaatggtat gcctgctatc
    17521 tggccctttg cagaaagcat gacagccgct gccttaagtt aaactctcta agtatgccaa
    17581 gggttttctt gtgtgttcgg gtgatacctt tatcttcaac acataacaaa acacagctgg
    17641 gtgctgaagg aatagttaat aagcagacaa aaaaaaaaaa aacagatcac ttaacctctg
    17701 ttttcttcat atgtaaaatg gggataacta tactgcaagt atctagtgac atttgacagc
    17761 aaaataagct aatacatgag aaagaatttc ccatgttatt ttaaaaagtg agaaaatgtt
    17821 cattgacagc ataatcctta ttaaaatagc agatgtttta taaaaataaa aaaaatccag
    17881 cttatttttt ttgtaagtta taaactcagg taagctatgt gacatttctg aaaatctgca
    17941 tcttaatcta tgagatcagg ataatgacag tatggggttg cagaaggaac aaacgggata
    18001 aagcaatgtg acatgctcgg cataatggct agaacaaatg gcacagtagt tggaactgtg
    18061 tttgaggctg actcacatgg cagacaaagg agcagttgag gcttggttga cttttggttg
    18121 ggagataggt ggctgggtac tgtcattttg gagaagggtg gatgggtact gtcatttgag
    18181 gaaagatgag accagtgata cagcttgtca gcacggtact tggtactgac tgaatgacaa
    18241 gaccttggtc tccactctca gtgctacaaa atgaaagcta aatgtagaca tggggtatat
    18301 tcagaaggtc aaagcaggag aatgaagaac ttagaagcca gtttaaggta cacaacaaga
    18361 tactatctaa aaagaaggaa aataaaaata aaacaggcag gcaggcagac aagaaggaag
    18421 gaaggaaggg agggggacgg gtacggggat ggagacggat gtagagcagg aaagaaccag
    18481 cagagactgc tataagatag taagcttagt tttaggggct gttaagagca ctgactattc
    18541 ttccagagga cccaggtttg agtcccagca cccacatggc agcccacaac catctgtaag
    18601 ccaagttcta aatgttccaa tgcccaattc tggccttcat gggcactgca tttatggggt
    18661 acacagacat acatgcagga aaatcacaca tttttaaaaa gacaataagc cagtcatatg
    18721 gagatcaggg atctaggaag atccaggtgt ctaacaagtg tggaatatgt gagtctcagg
    18781 tataatgata aaaatcttga agttaccagg aaaaagttat ctctgaaaat tcagaactca
    18841 aaacttaggg agccaaaaag aaagaaaacc cagacttagc atcaccccat gaatatcttt
    18901 ctgcctctca gagactccaa tgtctttgtt ttttcaggga aaagtaaaca catggatgaa
    18961 gccttgtgtt agtaggtcct cttacttaat ggagaaacac caaagacaag agatgtcccc
    19021 taagctagga tgttagataa agtaaactaa tcacactctc cggaacctaa aacatccaac
    19081 atcacccaat atttgttctg aaagaaaaaa aacaaaactc aatgatcttt tctattttaa
    19141 ggaaacagag ttgctaaaaa tatctcaata ttttgagaat ccatttttgg cattatggac
    19201 tgaggatgtt tattacctaa tgatctctat cttagtgcca tctcgatcaa tggcattcac
    19261 tcatcattac aatcatctga atgttagaga tgtacttcag atgatagaat ttgcctagca
    19321 tgtgtgcagc tccagacttc tctagagcct tgtaacttga atgtggtggt acaagcgtat
    19381 aatctcagca cccaagaagt agcagcaaga ggaagtttaa catcatcctc agatgcaatt
    19441 ttcagccagt ctgggtctta tgagataatg tctcaaaata aaatgataac ataaagttca
    19501 ctgaaataaa aacctatcac ttggaatttt ccaaaatgag aaatttgaaa aatataatac
    19561 atgaggcata cagatagatg taatacagag cacaatgatg cataccattc atcccagcat
    19621 ccaggaggca aggcaagcag atatcatcaa tgagtttaag gccagactgg tctacacgat
    19681 gttccaggcc agtcatgatc tataatgtct accagaagaa gtatgtcagt tcacctcatg
    19741 tggccttctt tgtcacacaa gacaatctga atcccagaat ccctgccaca aaatgtacac
    19801 cagaataagt cataagcctc acccagatcc ttcagtgtgg gaatggcatg tttgtccagg
    19861 gatgggagga attggaaagc tgtgtacacc aaaagtcttt ttctgtcacc taagatacac
    19921 actttccacc ttagtaatcc aagtacagat gaaacataca gtactgaggg gtgtctaggt
    19981 ggttcgatga aacaccaacc ccatctttgt tatgatgcca gtatgactag gagaattcca
    20041 gacatgtaca ttgtatacat tctatacatg gacaggtatc atgggttgtg atgaaagatg
    20101 aatgggatgg gagacagaca atggcatcct gtcttcatta ctcctttttt tcccatgaag
    20161 gtgtgtgtgt gtgtgtgtat gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgttgcc
    20221 atgtgtgtag gtacatatat atgtaagagt acacatgtac atgtttgcat gcaaaggcct
    20281 gaggctgatg tcaggtgtct tcctcgatct ctctgcctta ctcattgagt caggagctct
    20341 cctcaaaccc agagcccatc aatgtcacta gtcttgtgct agggaatcaa atctgacctt
    20401 tcaagcaaag gaatcacgat gggccaccac ggctccctgg catttacttg ggtgctggga
    20461 tacaaacttt ggtcctcatc tacagcccac atcactcttc ttaaaacaag aaaatgtctc
    20521 ctgtaggact ttatacctgt tgactataat tctcccaagc ctattcctaa accaatcact
    20581 ggaaagggaa aaaaccatcc tgattggctt agcttaattg tgactaagtg agaagcatgt
    20641 ggctacctag tacctgaaca aaacctgact tttgttagtt cagtaaaagg atgggatggc
    20701 tatttggtcc gctattagct gtttctgtca gagatactga aataatggac aataacaaca
    20761 aaggttttaa gtttgatctc aatatatctg gcctttaaat ctaaaggtct tgctctaatc
    20821 cctaaatcat gaaatggtat caacttagaa agactcaaat ctgtttttaa tttttttata
    20881 taaattacaa agacattgaa ataaagtata tgtgcttctg ttattgacat gacgagttac
    20941 cttggctgtc tttttggact acaaagaaat gagcttaaaa aggaggactt taccctgatc
    21001 tttagagtcc ctctaagatt cttgggagtt tctgctcagg gaagctctgc agtaggttca
    21061 gttcattatt cccccgtggt aagtgggacc ctagtagaga acaatcatac agagtcaact
    21121 gatctcccct cagtctatat cttggggttc atctgcagat cggcctctga agagcacaat
    21181 tgtgtggaga cgggaagtca agaaccaatg gccatttact tcaccagtgg gaccacaggc
    21241 tcccccaaga tggctcagca ctcacagggc agccttggca ttgggtatac cctttgtgga
    21301 aggtaggaaa ggaaactatg gttttgttct tattttcatt gcagcctggc ctcacactgt
    21361 agttggctct ggactataac tcactctgta cccttgactg gcctcaaatt catagcaatt
    21421 ctcctgcctc tcttccctcc cccacaaaaa tagttgaatt tgcaggagtg caccaccaca
    21481 ctgcttcttt gttttctatg cagtctccca ggctgccagt gtgggtgtcc aactgctgat
    21541 atgacacctc tccttgaatg gatattaaga tatctttaac gtagatccat aaaacagaac
    21601 tctggatgcc tctaccctac cctagctaaa tggtaccatt gaagtagctg atcaagcaag
    21661 aatgtaggtt tagtgcttga ttcatagcac ttctgcctct ctctctctcc ttatttccag
    21721 gtgaatttac cttcccaaaa gggtctttga aacaagtcta cctaaaactg ccacattgct
    21781 ctgtttaatt ttccttctac ctaaagttaa agcattcaat gattactctc aatcatcaac
    21841 ccaataaagt ctagaatacc caagggattg t
//