LOCUS       KO-first_condition_ready_31535_MGI:2681844        20815 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Acsm4 targeting project(s): 31535
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 42446
COMMENT     backbone: R3R4_pBR_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     misc_feature    161..3098
                     /note="pBR322"
     CDS             complement(2030..2890)
                     /note="AmpR"
     misc_feature    3109..3232
                     /note="R3 gateway site"
     misc_feature    3238..3245
                     /note="AsiSI"
     misc_feature    1..3270
                     /note="Synthetic Backbone"
     exon            3434..3677
                     /db_xref="ENSEMBL:ENSMUSE00000421258"
                     /rank="2"
                     /note="ENSMUSE00000421258"
     exon            4437..4647
                     /db_xref="ENSEMBL:ENSMUSE00000233168"
                     /rank="3"
                     /note="ENSMUSE00000233168"
     misc_feature    3271..7920
                     /note="5 arm"
     misc_feature    7948..7972
                     /note="B1 Gateway"
     misc_feature    7985..8032
                     /note="Frt"
     intron          8033..8907
                     /note="mouse En2 intron"
     misc_feature    8905..8907
                     /note="Splice Acceptor"
     exon            8908..9080
                     /note="mouse En2 exon"
     misc_feature    9092..9685
                     /note="ECMV IRES"
     gene            9686..12746
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    12785..13028
                     /note="SV40 polyadenylation site"
     misc_feature    13032..13065
                     /note="loxP"
     promoter        13092..13611
                     /note="human beta actin promoter"
     gene            13637..14431
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    14631..14872
                     /note="SV40 polyadenylation site"
     misc_feature    14889..14936
                     /note="Frt"
     misc_feature    14943..14976
                     /note="loxP"
     misc_feature    8922..8999
                     /note="FCHK std_gpr"
     misc_feature    14992..15012
                     /note="B2 Gateway"
     misc_feature    7921..15042
                     /note="Synthetic Cassette"
     exon            15412..15619
                     /db_xref="ENSEMBL:ENSMUSE00000400031"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000400031"
     misc_feature    15043..15773
                     /note="Critical Region"
     misc_feature    15797..15830
                     /note="Downstream LoxP"
     misc_feature    15774..15853
                     /note="synthetic loxP region"
     exon            20103..20246
                     /db_xref="ENSEMBL:ENSMUSE00000412236"
                     /rank="5"
                     /note="ENSMUSE00000412236"
     misc_feature    15854..20815
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga
      421 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc
      481 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
      541 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc
      601 atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa
      661 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg
      721 gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac
      781 cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
      841 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      901 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
      961 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     1021 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     1081 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1141 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1201 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1441 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1681 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     1741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2341 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2521 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2581 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2641 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2701 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2761 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2821 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2881 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2941 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3001 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3061 aaaataggcg tatcacgagg ccctttcgtc ttcaagaaaa ttgtcgacca tagtgactgg
     3121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3181 ttgatattta tatcatttta cgtttctcgt tcaactttat tatacaaagt tgcggacgcg
     3241 atcgccggcg gataacaatt tcacacagga attgttaggg ctaagctggt gacatgcaga
     3301 attaagaaac attcagtagt ggggacactg atcagtggct aatggtgaca gacctatgcg
     3361 aggatggagc gttcttcagg aatccactcc cagtcttcag ctaacactct cccctcctct
     3421 ctcttcccta caggcatttc ccacttgcta ccagaaaata aagtttttca ggaaccatga
     3481 aggttctcct ccactgtcag agacttagat tcatctggct tgccaagcca gccggccggc
     3541 acttccacag agattcccag ctctgggcac ctctgacact ggatgacttt gaagccatca
     3601 atcgctgtga gaagccatta cccaagaact ttaactttgc agcagatgtt ctggaccagt
     3661 ggtccctaaa ggagaaggta tcaaagagca gggccccagt gttcctgttg ctctcatctc
     3721 tgtcttccag gtcctctcac tgtgttctac ctgtcaagtt taaatcaatt tttttatctc
     3781 agtgagggca ggaacctgga tggcttcatg gagactcata agtacaagag cccataaaca
     3841 cctttcattc caatcagttt ctgtcttttg attcttctca gaaaacttag agtctaggga
     3901 gatgaattca gattaactct tccttggtat atgcttatcc cctctttaaa aagggcagac
     3961 cccaccaaga ggtgattctc caagactgtt ctccagtgtt ccactggaga gaaacatttc
     4021 tcccctaggc acactgggga agcagctctg gaggagtagc aatagacgtc ctatctgcct
     4081 tctgtgttag tcagtgtgcc atcactgtga caaaacacct aagacacaca atttcaagtg
     4141 aatgaaggtt gcttcggttc atgggtgcaa cgatatcagc ttcggttcac ttagccctgt
     4201 ggcagccctg agcagcacag ggaacatgca tggtgaggaa gactgcctgc tcccctccag
     4261 gacagctcag agaggtggaa ggaatgagtg agaggaaggg cctagggtct gatcgttttc
     4321 ttctttaagc cctaacgcca caggataggg gactatgcct tttaacatgt gggtcttcag
     4381 gtgacagctc taaaccataa tacattatct tctctttccc atgtgttcct ggccagtctg
     4441 gagagaggcc agccaaccca gccctgtggt gggtgaatgg gaaaggagat gaggtgaagt
     4501 ggagcttcca agaactgggt tccttgtccc gaaaggctgc caatgtgctc accaagccct
     4561 gcggcctgca gagaggagac cgggtggctg tgattctgcc ccgtatccct gaatggtggc
     4621 tcatcaatgt ggcttgcatg agaacaggtg agtatcacaa atgagtgtca aaacttgaga
     4681 cttttgtgaa gggtcagctt tgactaaagc aatccttaca aaaagtagag aaagaaagtt
     4741 aaccttaata gcagttgtga tgaaataata acaacaaact taagaaattg ggaacactgt
     4801 gataaccatt ttttgagaga ggaaatcaaa gcacagtgat attaagacat ttgccaaagg
     4861 tcacatcact tgaaaattat gtaattgggg acctgggaag atgactcagt ggataaagta
     4921 cttgctgtac aaacatgtgg ccctgcattc ccacatcaaa agcttggcat ggagggacag
     4981 gtttctatga tcccagcact gtgggcgcag agacaggcac atcccctggt ctcactggcc
     5041 agccacacct agtcaagaca gaaagtctca ggttcacaca catataccca cacacacaaa
     5101 taaaaatgaa aaagagagtt gcactattca gagttgcaga aaaaagctct ataaagcaaa
     5161 tgcaagtgtg agtgtttctc cacagcacag ggtgtgtgga atatcccccc catacacaca
     5221 cacacacaca cacacacaca cacacacaca cacacacgcg cgcacacacg cgcgcacaca
     5281 cacacacacg cacacacacg gttctctctt ctgggtgtgt atctagattc tctgcaacac
     5341 aaaaatacag aagaagtgga agtctttgag gatgcaaaga tttgtaagaa cataagggac
     5401 tctggaccca agtttggaaa aaaaaaaaaa actccagtca gaagaactaa tgtatccagt
     5461 tacacatttt ccccaaagta gtaacatgta aagtaaagac atgtctttca gataggggtc
     5521 tgtgagccat gaactatggt tcctctgctt gggtaaatag acgtgtgatg gaatacagct
     5581 gactcctttt gctccacagc aaacgtgctg actgagacca agaacaatcc tacaggccac
     5641 agagtctaac acactctcca ggccttttac caaaagactc tctgatttct tatctttaaa
     5701 aaaaaaaaat catgccgggc atggtggcac atgcctttaa ttccagcact caggaggcag
     5761 aggcagtcag acttctgagt tcaaggccag cctggtctac aaagtgagtt ctaggacagc
     5821 cagggctata cagagaaacc ctgcctcgaa aaaccaacaa aaaattaaaa aataaaaaaa
     5881 atcatcagac atccattaaa ataaataaga atgccaaagt gtagactact acagagaaca
     5941 gaaagatatc tgtgcaatgt tattagcagg tatacaaaac caaacttaaa tagaaatgat
     6001 taaacaatgc ataacctagc taaggtgtaa gtttagttga gttgttgatt gatggttgaa
     6061 tggcttgatc ttgattaaaa aataatttat actagcaata ataacatcag gagaatttca
     6121 gagaatttaa gatgctttta atgccaataa aatacatgaa agcaggagta taaaaatgtt
     6181 taaattcacc atataaaaag acagagataa attggaaatc agccgtggtt ggaagaagat
     6241 taaggatggt ttatctgtta tgaaatatac ccttaatgta atttaaagta aattatttaa
     6301 cttaggaaaa tctgtgagta caaacacaca agctagtgtt caaaaacatg aaataaaaca
     6361 ataacaacag ttcatcaagt gaagaaagcc agctgcagac ctgcttattc cccaaaatgt
     6421 caaacccttc atgtgaattt ttaaaattgc cttcactcac cacataacaa actatttgat
     6481 tttgctggaa ggcatatgca ataaagtcta aaatacttgc catccaacaa acatttgctg
     6541 tctaaaaccc attagaagaa atcttcctct gactcccaag gtggtaatct actactgaac
     6601 acattcataa atccaccacg gagactgtaa ctgggaatca ttaagagcaa ataacttgta
     6661 agagaaatca ttttatattt ttatattaat tgatcgttct gagactctct aacgtgtttt
     6721 cttaagggaa taactcagct aactcagtta aaatatactc agtaaaatag ccttgggatg
     6781 gcaagatggc tctgcaggta aaggtgcttg ctatgcaagt ctggccacca gagcttctga
     6841 gtccatgaga ccaacctaaa ggtggaagga gagcgttgat attacaaaag gtgtcctctg
     6901 cccatatgcg taccacagca tgagagctcc ccacaataac actgatgaca gtaaaaaaag
     6961 agcctccttc ctaggaattc aactccacaa cttcctccta tttagctgat ttcagaatac
     7021 ccagcaagga gtacatacat agacatacaa cacaggtctt gggagtgata gaaatgtctc
     7081 tggggactaa ccttgctatt ggcattgcca tttattggct ttgtgacccg taggtgcgtt
     7141 atcattcact ggcatgtgat atgtgctctg tgcacggcag tggctatctg taatgttttg
     7201 agtatcactg tcttcccatg gattcacttt agagagtcaa accacctgct tacatcttat
     7261 ccttacatct aatccttaca ccatacatgt aatcctgccc tgtgcagtct tctcataagg
     7321 agctttgaaa tcctgtctct ggctgctgtt tgggatgaag agggataaat taagcatgac
     7381 catcgtcatc taggaaaatc ttggcggtac cagaccacgg catcaatgat ttgtaggctg
     7441 gctctgccac tcaccagcta tccccaaaga ctgcatgcac ctcgtgggga tgcttaaaac
     7501 agtagtttca ggttactaag gtggcttggt tactaaagat gcttgctgca cgagcctagc
     7561 tacatgagtt agattcctag gacccacaaa aaggtacaag gaaagaacca catccacaat
     7621 ggtatcatct gacctccatg caaacaacac acacacatac acacacacac acacacaaat
     7681 gatagattaa aaaaaatcct tttaagtttt tgaagaatag tctctgagca tctggggcta
     7741 tgcaatctat caaaaaaaat taatgagcag gcattttcct agctgcatat aatttttcac
     7801 tctgtcctgt tgcactgatg tagtatgcat cttacaaagc atttcaacaa agagaagaat
     7861 tctagtgtct ctgtaccttt tacatatcta ttatttgctt ttctcattcc cagataaatt
     7921 aaggcgcata acgataccac gatatcaaca agtttgtaca aaaaagcagg ctggcgccgg
     7981 aaccgaagtt cctattccga agttcctatt ctctagaaag tataggaact tcgaaccctt
     8041 tcccacacca ccctccacac ttgccccaaa cactgccaac tatgtaggag gaaggggttg
     8101 ggactaacag aagaacccgt tgtggggaag ctgttgggag ggtcacttta tgttcttgcc
     8161 caaggtcagt tgggtggcct gcttctgatg aggtggtccc aaggtctggg gtagaaggtg
     8221 agagggacag gccaccaagg tcagcccccc ccccctatcc cataggagcc aggtccctct
     8281 cctggacagg aagactgaag gggagatgcc agagactcag tgaagcctgg ggtaccctat
     8341 tggagtcctt caaggaaaca aacttggcct caccaggcct cagccttggc tcctcctggg
     8401 aactctactg cccttgggat ccccttgtag ttgtgggtta cataggaagg gggacgggat
     8461 tccccttgac tggctagcct actcttttct tcagtcttct ccatctcctc tcacctgtct
     8521 ctcgaccctt tccctaggat agacttggaa aaagataagg ggagaaaaca aatgcaaacg
     8581 aggccagaaa gattttggct gggcattcct tccgctagct tttattggga tcccctagtt
     8641 tgtgataggc cttttagcta catctgccaa tccatctcat tttcacacac acacacacca
     8701 ctttccttct ggtcagtggg cacatgtcca gcctcaagtt tatatcacca cccccaatgc
     8761 ccaacacttg tatggccttg ggcgggtcat cccccccccc acccccagta tctgcaacct
     8821 caagctagct tgggtgcgtt ggttgtggat aagtagctag actccagcaa ccagtaacct
     8881 ctgccctttc tcctccatga caaccaggtc ccaggtcccg aaaaccaaag aagaagaacc
     8941 ctaacaaaga ggacaagcgg cctcgcacag ccttcactgc tgagcagctc cagaggctca
     9001 aggctgagtt tcagaccaac aggtacctga cagagcagcg gcgccagagt ctggcacagg
     9061 agctcggtac ccggaagatc tggactctag agaattccgc ccctctccct cccccccccc
     9121 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     9181 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     9241 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     9301 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     9361 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     9421 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     9481 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     9541 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     9601 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     9661 acacgatgat aagcttgcca caaccatgga agatcccgtc gttttacaac gtcgtgactg
     9721 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     9781 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     9841 cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg
     9901 cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga
     9961 tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac
    10021 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga
    10081 aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg
    10141 gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt
    10201 tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta
    10261 tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca
    10321 taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag
    10381 ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt
    10441 aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg
    10501 tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga
    10561 aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca
    10621 caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg
    10681 gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg
    10741 tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat
    10801 cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc
    10861 gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga
    10921 aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat
    10981 gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg
    11041 gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa
    11101 atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac
    11161 cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc
    11221 gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg
    11281 cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt
    11341 tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat
    11401 taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc
    11461 gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc
    11521 gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat
    11581 cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt
    11641 ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg
    11701 taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct
    11761 cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc
    11821 ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc
    11881 catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg
    11941 gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact
    12001 gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag
    12061 tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta
    12121 ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat
    12181 tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta
    12241 ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac
    12301 accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa
    12361 ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga
    12421 ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg
    12481 tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca
    12541 gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct
    12601 gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga
    12661 cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta
    12721 ccagttggtc tggtgtcaaa aataataata accgggcagg ggggatctaa gctctagata
    12781 agtaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct
    12841 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    12901 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    12961 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    13021 ctggatccgg aataacttcg tatagcatac attatacgaa gttatgttta aacggcgcgc
    13081 cccggaattc gccttctgca ggagcgtaca gaacccaggg ccctggcacc cgtgcagacc
    13141 ctggcccacc ccacctgggc gctcagtgcc caagagatgt ccacacctag gatgtcccgc
    13201 ggtgggtggg gggcccgaga gacgggcagg ccgggggcag gcctggccat gcggggccga
    13261 accgggcact gcccagcgtg ggcgcggggg ccacggcgcg cgcccccagc ccccgggccc
    13321 agcaccccaa ggcggccaac gccaaaactc tccctcctcc tcttcctcaa tctcgctctc
    13381 gctctttttt tttttcgcaa aaggagggga gagggggtaa aaaaatgctg cactgtgcgg
    13441 cgaagccggt gagtgagcgg cgcggggcca atcagcgtgc gccgttccga aagttgcctt
    13501 ttatggctcg agcggccgcg gcggcgccct ataaaaccca gcggcgcgac gcgccaccac
    13561 cgccgagacc gcgtccgccc cgcgagcaca gagcctcgcc tttgccgatc ctctagagtc
    13621 gagatccgcc gccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
    13681 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
    13741 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
    13801 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
    13861 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
    13921 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
    13981 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
    14041 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
    14101 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
    14161 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
    14221 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
    14281 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
    14341 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
    14401 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
    14461 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
    14521 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
    14581 atgctggagt tcttcgccca ccccccggat ctaagctcta gataagtaat gatcataatc
    14641 agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    14701 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    14761 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    14821 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgggggtac
    14881 cgcgtcgaga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcgtcg
    14941 agataacttc gtatagcata cattatacga agttatgtcg agatatctag acccagcttt
    15001 cttgtacaaa gtggttgata tctctatagt cgcagtaggc ggattaaata tcacacaatc
    15061 attacacaat cacctttcaa gcctgacatg aaagtggttc ttactactca gcccatcatt
    15121 gtcaaccagc tgctgtcatt atccccatga taaaagtaaa gaagggaagg ccagagaaac
    15181 ccaagtcata gggctagcaa gttgcaagac caagactaga cccaaggcct gtcagattcc
    15241 aaagccagag ctctactgat ccctcatgcc agggcccagc aggacagccc agggaaccaa
    15301 gttacaggta gctgagggct gacatctgaa gttctcattc atccagacac aagacctaga
    15361 tttcccaata atctccagag ctgttagctc aggaatcact ttttgttcca gggcttgtat
    15421 tcatgccagg aacgatccaa ctgaccagaa aagacatcct ctaccggctg caagcatcca
    15481 aggccaagtg cattgtggct agtgaggaag tggcccccgc agtggactcc attgtatcag
    15541 agtgtcccag cctgaagact aaactcctgg tgtctccaca ccactgggat ggatggctca
    15601 actttcagga gttacttcag tgagtatttg ggtgggaggc actagcaaca gcacaaatca
    15661 caggtacagc tctgaatctg tccgtccaaa gactcagcaa tagccagagt tgataccggg
    15721 ctccttccca aaacagaatg tttttaagag tatgagctta tgagtacatg tgtgagatgg
    15781 cgcaacgcaa ttaatgataa cttcgtatag catacattat acgaagttat ggtctgagct
    15841 cgccatcagt tcagaacata tgtcctctgt aataataata ggtactctta gaccctagac
    15901 catctctcca gctccctaaa aagtcattta atgaaattat ttacagacag aagaaatatg
    15961 tacagctcta ctctgacttt tctgtctcct ctgtggccta tgatgagtgt gctatagaga
    16021 tgggcctttg ggacacaact atcatgatct ctcttttact ctttgaacat gctctagctc
    16081 agaaccttgg catccactgt ctctcctgcc ccgatgttct ttcctagtcc tttctatagt
    16141 tgttctatct tgtcaggttt cagttcaaat gttttatcct acgtgatgct gctcctgacc
    16201 atcttgatgg aagccgtaag gcttttttct ccatgcatgg actgtttctt cgcgttgcta
    16261 ggtcctctga gtgatcagac tagcctcgtg ttcttttaga ttcagttttt ctactccaag
    16321 gtttggactg gaaaattagg ctccaaagcc aaatcgggtc caccacttgc ttttgtaaat
    16381 aaagtgtcat tggcacacag aggtgctcat ttgtttgtat attgtctatg tctgctttca
    16441 tttagaaatg gtatgcctgc tatctggccc tttgcagaaa gcatgacagc cgctgcctta
    16501 agttaaactc tctaagtatg ccaagggttt tcttgtgtgt tcgggtgata cctttatctt
    16561 caacacataa caaaacacag ctgggtgctg aaggaatagt taataagcag acaaaaaaaa
    16621 aaaaaacaga tcacttaacc tctgttttct tcatatgtaa aatggggata actatactgc
    16681 aagtatctag tgacatttga cagcaaaata agctaataca tgagaaagaa tttcccatgt
    16741 tattttaaaa agtgagaaaa tgttcattga cagcataatc cttattaaaa tagcagatgt
    16801 tttataaaaa taaaaaaaat ccagcttatt ttttttgtaa gttataaact caggtaagct
    16861 atgtgacatt tctgaaaatc tgcatcttaa tctatgagat caggataatg acagtatggg
    16921 gttgcagaag gaacaaacgg gataaagcaa tgtgacatgc tcggcataat ggctagaaca
    16981 aatggcacag tagttggaac tgtgtttgag gctgactcac atggcagaca aaggagcagt
    17041 tgaggcttgg ttgacttttg gttgggagat aggtggctgg gtactgtcat tttggagaag
    17101 ggtggatggg tactgtcatt tgaggaaaga tgagaccagt gatacagctt gtcagcacgg
    17161 tacttggtac tgactgaatg acaagacctt ggtctccact ctcagtgcta caaaatgaaa
    17221 gctaaatgta gacatggggt atattcagaa ggtcaaagca ggagaatgaa gaacttagaa
    17281 gccagtttaa ggtacacaac aagatactat ctaaaaagaa ggaaaataaa aataaaacag
    17341 gcaggcaggc agacaagaag gaaggaagga agggaggggg acgggtacgg ggatggagac
    17401 ggatgtagag caggaaagaa ccagcagaga ctgctataag atagtaagct tagttttagg
    17461 ggctgttaag agcactgact attcttccag aggacccagg tttgagtccc agcacccaca
    17521 tggcagccca caaccatctg taagccaagt tctaaatgtt ccaatgccca attctggcct
    17581 tcatgggcac tgcatttatg gggtacacag acatacatgc aggaaaatca cacattttta
    17641 aaaagacaat aagccagtca tatggagatc agggatctag gaagatccag gtgtctaaca
    17701 agtgtggaat atgtgagtct caggtataat gataaaaatc ttgaagttac caggaaaaag
    17761 ttatctctga aaattcagaa ctcaaaactt agggagccaa aaagaaagaa aacccagact
    17821 tagcatcacc ccatgaatat ctttctgcct ctcagagact ccaatgtctt tgttttttca
    17881 gggaaaagta aacacatgga tgaagccttg tgttagtagg tcctcttact taatggagaa
    17941 acaccaaaga caagagatgt cccctaagct aggatgttag ataaagtaaa ctaatcacac
    18001 tctccggaac ctaaaacatc caacatcacc caatatttgt tctgaaagaa aaaaaacaaa
    18061 actcaatgat cttttctatt ttaaggaaac agagttgcta aaaatatctc aatattttga
    18121 gaatccattt ttggcattat ggactgagga tgtttattac ctaatgatct ctatcttagt
    18181 gccatctcga tcaatggcat tcactcatca ttacaatcat ctgaatgtta gagatgtact
    18241 tcagatgata gaatttgcct agcatgtgtg cagctccaga cttctctaga gccttgtaac
    18301 ttgaatgtgg tggtacaagc gtataatctc agcacccaag aagtagcagc aagaggaagt
    18361 ttaacatcat cctcagatgc aattttcagc cagtctgggt cttatgagat aatgtctcaa
    18421 aataaaatga taacataaag ttcactgaaa taaaaaccta tcacttggaa ttttccaaaa
    18481 tgagaaattt gaaaaatata atacatgagg catacagata gatgtaatac agagcacaat
    18541 gatgcatacc attcatccca gcatccagga ggcaaggcaa gcagatatca tcaatgagtt
    18601 taaggccaga ctggtctaca cgatgttcca ggccagtcat gatctataat gtctaccaga
    18661 agaagtatgt cagttcacct catgtggcct tctttgtcac acaagacaat ctgaatccca
    18721 gaatccctgc cacaaaatgt acaccagaat aagtcataag cctcacccag atccttcagt
    18781 gtgggaatgg catgtttgtc cagggatggg aggaattgga aagctgtgta caccaaaagt
    18841 ctttttctgt cacctaagat acacactttc caccttagta atccaagtac agatgaaaca
    18901 tacagtactg aggggtgtct aggtggttcg atgaaacacc aaccccatct ttgttatgat
    18961 gccagtatga ctaggagaat tccagacatg tacattgtat acattctata catggacagg
    19021 tatcatgggt tgtgatgaaa gatgaatggg atgggagaca gacaatggca tcctgtcttc
    19081 attactcctt tttttcccat gaaggtgtgt gtgtgtgtgt gtatgtgtgt gtgtgtgtgt
    19141 gtgtgtgtgt gtgtgtgtgt tgccatgtgt gtaggtacat atatatgtaa gagtacacat
    19201 gtacatgttt gcatgcaaag gcctgaggct gatgtcaggt gtcttcctcg atctctctgc
    19261 cttactcatt gagtcaggag ctctcctcaa acccagagcc catcaatgtc actagtcttg
    19321 tgctagggaa tcaaatctga cctttcaagc aaaggaatca cgatgggcca ccacggctcc
    19381 ctggcattta cttgggtgct gggatacaaa ctttggtcct catctacagc ccacatcact
    19441 cttcttaaaa caagaaaatg tctcctgtag gactttatac ctgttgacta taattctccc
    19501 aagcctattc ctaaaccaat cactggaaag ggaaaaaacc atcctgattg gcttagctta
    19561 attgtgacta agtgagaagc atgtggctac ctagtacctg aacaaaacct gacttttgtt
    19621 agttcagtaa aaggatggga tggctatttg gtccgctatt agctgtttct gtcagagata
    19681 ctgaaataat ggacaataac aacaaaggtt ttaagtttga tctcaatata tctggccttt
    19741 aaatctaaag gtcttgctct aatccctaaa tcatgaaatg gtatcaactt agaaagactc
    19801 aaatctgttt ttaatttttt tatataaatt acaaagacat tgaaataaag tatatgtgct
    19861 tctgttattg acatgacgag ttaccttggc tgtctttttg gactacaaag aaatgagctt
    19921 aaaaaggagg actttaccct gatctttaga gtccctctaa gattcttggg agtttctgct
    19981 cagggaagct ctgcagtagg ttcagttcat tattcccccg tggtaagtgg gaccctagta
    20041 gagaacaatc atacagagtc aactgatctc ccctcagtct atatcttggg gttcatctgc
    20101 agatcggcct ctgaagagca caattgtgtg gagacgggaa gtcaagaacc aatggccatt
    20161 tacttcacca gtgggaccac aggctccccc aagatggctc agcactcaca gggcagcctt
    20221 ggcattgggt ataccctttg tggaaggtag gaaaggaaac tatggttttg ttcttatttt
    20281 cattgcagcc tggcctcaca ctgtagttgg ctctggacta taactcactc tgtacccttg
    20341 actggcctca aattcatagc aattctcctg cctctcttcc ctcccccaca aaaatagttg
    20401 aatttgcagg agtgcaccac cacactgctt ctttgttttc tatgcagtct cccaggctgc
    20461 cagtgtgggt gtccaactgc tgatatgaca cctctccttg aatggatatt aagatatctt
    20521 taacgtagat ccataaaaca gaactctgga tgcctctacc ctaccctagc taaatggtac
    20581 cattgaagta gctgatcaag caagaatgta ggtttagtgc ttgattcata gcacttctgc
    20641 ctctctctct ctccttattt ccaggtgaat ttaccttccc aaaagggtct ttgaaacaag
    20701 tctacctaaa actgccacat tgctctgttt aattttcctt ctacctaaag ttaaagcatt
    20761 caatgattac tctcaatcat caacccaata aagtctagaa tacccaaggg attgt
//