LOCUS       KO-first_condition_ready_31534_MGI:2681844        21819 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Acsm4 targeting project(s): 31534
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 42446
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            4438..4681
                     /db_xref="ENSEMBL:ENSMUSE00000421258"
                     /rank="2"
                     /note="ENSMUSE00000421258"
     exon            5441..5651
                     /db_xref="ENSEMBL:ENSMUSE00000233168"
                     /rank="3"
                     /note="ENSMUSE00000233168"
     misc_feature    4275..8924
                     /note="5 arm"
     misc_feature    8952..8976
                     /note="B1 Gateway"
     misc_feature    8989..9036
                     /note="Frt"
     intron          9037..9911
                     /note="mouse En2 intron"
     misc_feature    9909..9911
                     /note="Splice Acceptor"
     exon            9912..10084
                     /note="mouse En2 exon"
     misc_feature    10096..10689
                     /note="ECMV IRES"
     gene            10690..13750
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13789..14032
                     /note="SV40 polyadenylation site"
     misc_feature    14036..14069
                     /note="loxP"
     promoter        14096..14615
                     /note="human beta actin promoter"
     gene            14641..15435
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15635..15876
                     /note="SV40 polyadenylation site"
     misc_feature    15893..15940
                     /note="Frt"
     misc_feature    15947..15980
                     /note="loxP"
     misc_feature    9926..10003
                     /note="FCHK std_gpr"
     misc_feature    15996..16016
                     /note="B2 Gateway"
     misc_feature    8925..16046
                     /note="Synthetic Cassette"
     exon            16416..16623
                     /db_xref="ENSEMBL:ENSMUSE00000400031"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000400031"
     misc_feature    16047..16777
                     /note="Critical Region"
     misc_feature    16801..16834
                     /note="Downstream LoxP"
     misc_feature    16778..16857
                     /note="synthetic loxP region"
     exon            21107..21250
                     /db_xref="ENSEMBL:ENSMUSE00000412236"
                     /rank="5"
                     /note="ENSMUSE00000412236"
     misc_feature    16858..21819
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaattgtt agggctaagc tggtgacatg cagaattaag aaacattcag
     4321 tagtggggac actgatcagt ggctaatggt gacagaccta tgcgaggatg gagcgttctt
     4381 caggaatcca ctcccagtct tcagctaaca ctctcccctc ctctctcttc cctacaggca
     4441 tttcccactt gctaccagaa aataaagttt ttcaggaacc atgaaggttc tcctccactg
     4501 tcagagactt agattcatct ggcttgccaa gccagccggc cggcacttcc acagagattc
     4561 ccagctctgg gcacctctga cactggatga ctttgaagcc atcaatcgct gtgagaagcc
     4621 attacccaag aactttaact ttgcagcaga tgttctggac cagtggtccc taaaggagaa
     4681 ggtatcaaag agcagggccc cagtgttcct gttgctctca tctctgtctt ccaggtcctc
     4741 tcactgtgtt ctacctgtca agtttaaatc aattttttta tctcagtgag ggcaggaacc
     4801 tggatggctt catggagact cataagtaca agagcccata aacacctttc attccaatca
     4861 gtttctgtct tttgattctt ctcagaaaac ttagagtcta gggagatgaa ttcagattaa
     4921 ctcttccttg gtatatgctt atcccctctt taaaaagggc agaccccacc aagaggtgat
     4981 tctccaagac tgttctccag tgttccactg gagagaaaca tttctcccct aggcacactg
     5041 gggaagcagc tctggaggag tagcaataga cgtcctatct gccttctgtg ttagtcagtg
     5101 tgccatcact gtgacaaaac acctaagaca cacaatttca agtgaatgaa ggttgcttcg
     5161 gttcatgggt gcaacgatat cagcttcggt tcacttagcc ctgtggcagc cctgagcagc
     5221 acagggaaca tgcatggtga ggaagactgc ctgctcccct ccaggacagc tcagagaggt
     5281 ggaaggaatg agtgagagga agggcctagg gtctgatcgt tttcttcttt aagccctaac
     5341 gccacaggat aggggactat gccttttaac atgtgggtct tcaggtgaca gctctaaacc
     5401 ataatacatt atcttctctt tcccatgtgt tcctggccag tctggagaga ggccagccaa
     5461 cccagccctg tggtgggtga atgggaaagg agatgaggtg aagtggagct tccaagaact
     5521 gggttccttg tcccgaaagg ctgccaatgt gctcaccaag ccctgcggcc tgcagagagg
     5581 agaccgggtg gctgtgattc tgccccgtat ccctgaatgg tggctcatca atgtggcttg
     5641 catgagaaca ggtgagtatc acaaatgagt gtcaaaactt gagacttttg tgaagggtca
     5701 gctttgacta aagcaatcct tacaaaaagt agagaaagaa agttaacctt aatagcagtt
     5761 gtgatgaaat aataacaaca aacttaagaa attgggaaca ctgtgataac cattttttga
     5821 gagaggaaat caaagcacag tgatattaag acatttgcca aaggtcacat cacttgaaaa
     5881 ttatgtaatt ggggacctgg gaagatgact cagtggataa agtacttgct gtacaaacat
     5941 gtggccctgc attcccacat caaaagcttg gcatggaggg acaggtttct atgatcccag
     6001 cactgtgggc gcagagacag gcacatcccc tggtctcact ggccagccac acctagtcaa
     6061 gacagaaagt ctcaggttca cacacatata cccacacaca caaataaaaa tgaaaaagag
     6121 agttgcacta ttcagagttg cagaaaaaag ctctataaag caaatgcaag tgtgagtgtt
     6181 tctccacagc acagggtgtg tggaatatcc cccccataca cacacacaca cacacacaca
     6241 cacacacaca cacacacaca cgcgcgcaca cacgcgcgca cacacacaca cacgcacaca
     6301 cacggttctc tcttctgggt gtgtatctag attctctgca acacaaaaat acagaagaag
     6361 tggaagtctt tgaggatgca aagatttgta agaacataag ggactctgga cccaagtttg
     6421 gaaaaaaaaa aaaaactcca gtcagaagaa ctaatgtatc cagttacaca ttttccccaa
     6481 agtagtaaca tgtaaagtaa agacatgtct ttcagatagg ggtctgtgag ccatgaacta
     6541 tggttcctct gcttgggtaa atagacgtgt gatggaatac agctgactcc ttttgctcca
     6601 cagcaaacgt gctgactgag accaagaaca atcctacagg ccacagagtc taacacactc
     6661 tccaggcctt ttaccaaaag actctctgat ttcttatctt taaaaaaaaa aaatcatgcc
     6721 gggcatggtg gcacatgcct ttaattccag cactcaggag gcagaggcag tcagacttct
     6781 gagttcaagg ccagcctggt ctacaaagtg agttctagga cagccagggc tatacagaga
     6841 aaccctgcct cgaaaaacca acaaaaaatt aaaaaataaa aaaaatcatc agacatccat
     6901 taaaataaat aagaatgcca aagtgtagac tactacagag aacagaaaga tatctgtgca
     6961 atgttattag caggtataca aaaccaaact taaatagaaa tgattaaaca atgcataacc
     7021 tagctaaggt gtaagtttag ttgagttgtt gattgatggt tgaatggctt gatcttgatt
     7081 aaaaaataat ttatactagc aataataaca tcaggagaat ttcagagaat ttaagatgct
     7141 tttaatgcca ataaaataca tgaaagcagg agtataaaaa tgtttaaatt caccatataa
     7201 aaagacagag ataaattgga aatcagccgt ggttggaaga agattaagga tggtttatct
     7261 gttatgaaat atacccttaa tgtaatttaa agtaaattat ttaacttagg aaaatctgtg
     7321 agtacaaaca cacaagctag tgttcaaaaa catgaaataa aacaataaca acagttcatc
     7381 aagtgaagaa agccagctgc agacctgctt attccccaaa atgtcaaacc cttcatgtga
     7441 atttttaaaa ttgccttcac tcaccacata acaaactatt tgattttgct ggaaggcata
     7501 tgcaataaag tctaaaatac ttgccatcca acaaacattt gctgtctaaa acccattaga
     7561 agaaatcttc ctctgactcc caaggtggta atctactact gaacacattc ataaatccac
     7621 cacggagact gtaactggga atcattaaga gcaaataact tgtaagagaa atcattttat
     7681 atttttatat taattgatcg ttctgagact ctctaacgtg ttttcttaag ggaataactc
     7741 agctaactca gttaaaatat actcagtaaa atagccttgg gatggcaaga tggctctgca
     7801 ggtaaaggtg cttgctatgc aagtctggcc accagagctt ctgagtccat gagaccaacc
     7861 taaaggtgga aggagagcgt tgatattaca aaaggtgtcc tctgcccata tgcgtaccac
     7921 agcatgagag ctccccacaa taacactgat gacagtaaaa aaagagcctc cttcctagga
     7981 attcaactcc acaacttcct cctatttagc tgatttcaga atacccagca aggagtacat
     8041 acatagacat acaacacagg tcttgggagt gatagaaatg tctctgggga ctaaccttgc
     8101 tattggcatt gccatttatt ggctttgtga cccgtaggtg cgttatcatt cactggcatg
     8161 tgatatgtgc tctgtgcacg gcagtggcta tctgtaatgt tttgagtatc actgtcttcc
     8221 catggattca ctttagagag tcaaaccacc tgcttacatc ttatccttac atctaatcct
     8281 tacaccatac atgtaatcct gccctgtgca gtcttctcat aaggagcttt gaaatcctgt
     8341 ctctggctgc tgtttgggat gaagagggat aaattaagca tgaccatcgt catctaggaa
     8401 aatcttggcg gtaccagacc acggcatcaa tgatttgtag gctggctctg ccactcacca
     8461 gctatcccca aagactgcat gcacctcgtg gggatgctta aaacagtagt ttcaggttac
     8521 taaggtggct tggttactaa agatgcttgc tgcacgagcc tagctacatg agttagattc
     8581 ctaggaccca caaaaaggta caaggaaaga accacatcca caatggtatc atctgacctc
     8641 catgcaaaca acacacacac atacacacac acacacacac aaatgataga ttaaaaaaaa
     8701 tccttttaag tttttgaaga atagtctctg agcatctggg gctatgcaat ctatcaaaaa
     8761 aaattaatga gcaggcattt tcctagctgc atataatttt tcactctgtc ctgttgcact
     8821 gatgtagtat gcatcttaca aagcatttca acaaagagaa gaattctagt gtctctgtac
     8881 cttttacata tctattattt gcttttctca ttcccagata aattaaggcg cataacgata
     8941 ccacgatatc aacaagtttg tacaaaaaag caggctggcg ccggaaccga agttcctatt
     9001 ccgaagttcc tattctctag aaagtatagg aacttcgaac cctttcccac accaccctcc
     9061 acacttgccc caaacactgc caactatgta ggaggaaggg gttgggacta acagaagaac
     9121 ccgttgtggg gaagctgttg ggagggtcac tttatgttct tgcccaaggt cagttgggtg
     9181 gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa ggtgagaggg acaggccacc
     9241 aaggtcagcc ccccccccct atcccatagg agccaggtcc ctctcctgga caggaagact
     9301 gaaggggaga tgccagagac tcagtgaagc ctggggtacc ctattggagt ccttcaagga
     9361 aacaaacttg gcctcaccag gcctcagcct tggctcctcc tgggaactct actgcccttg
     9421 ggatcccctt gtagttgtgg gttacatagg aagggggacg ggattcccct tgactggcta
     9481 gcctactctt ttcttcagtc ttctccatct cctctcacct gtctctcgac cctttcccta
     9541 ggatagactt ggaaaaagat aaggggagaa aacaaatgca aacgaggcca gaaagatttt
     9601 ggctgggcat tccttccgct agcttttatt gggatcccct agtttgtgat aggcctttta
     9661 gctacatctg ccaatccatc tcattttcac acacacacac accactttcc ttctggtcag
     9721 tgggcacatg tccagcctca agtttatatc accaccccca atgcccaaca cttgtatggc
     9781 cttgggcggg tcatcccccc ccccaccccc agtatctgca acctcaagct agcttgggtg
     9841 cgttggttgt ggataagtag ctagactcca gcaaccagta acctctgccc tttctcctcc
     9901 atgacaacca ggtcccaggt cccgaaaacc aaagaagaag aaccctaaca aagaggacaa
     9961 gcggcctcgc acagccttca ctgctgagca gctccagagg ctcaaggctg agtttcagac
    10021 caacaggtac ctgacagagc agcggcgcca gagtctggca caggagctcg gtacccggaa
    10081 gatctggact ctagagaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
    10141 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
    10201 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
    10261 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
    10321 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
    10381 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
    10441 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
    10501 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
    10561 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac
    10621 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt
    10681 gccacaacca tggaagatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    10741 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    10801 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc
    10861 tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc
    10921 gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc
    10981 aacgtgacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt
    11041 tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt
    11101 atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac
    11161 ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa
    11221 aaccgcctcg cggtgatggt gctgcgctgg agtgacggca gttatctgga agatcaggat
    11281 atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa
    11341 atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag
    11401 gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg
    11461 cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag
    11521 cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg
    11581 agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg
    11641 ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg
    11701 ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct
    11761 ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag
    11821 aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg
    11881 tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg
    11941 ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg
    12001 cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa
    12061 tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc
    12121 cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg
    12181 atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa
    12241 aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg
    12301 atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt
    12361 ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac
    12421 ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc
    12481 tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa
    12541 caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa
    12601 tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag
    12661 ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa
    12721 ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg
    12781 caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt
    12841 ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc
    12901 accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag
    12961 tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc
    13021 gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt
    13081 gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg
    13141 ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg
    13201 tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt
    13261 ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg
    13321 attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg
    13381 ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca
    13441 ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg
    13501 cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc
    13561 tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc
    13621 acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg
    13681 tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt
    13741 caaaaataat aataaccggg caggggggat ctaagctcta gataagtaat gatcataatc
    13801 agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    13861 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    13921 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    13981 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccggaataac
    14041 ttcgtatagc atacattata cgaagttatg tttaaacggc gcgccccgga attcgccttc
    14101 tgcaggagcg tacagaaccc agggccctgg cacccgtgca gaccctggcc caccccacct
    14161 gggcgctcag tgcccaagag atgtccacac ctaggatgtc ccgcggtggg tggggggccc
    14221 gagagacggg caggccgggg gcaggcctgg ccatgcgggg ccgaaccggg cactgcccag
    14281 cgtgggcgcg ggggccacgg cgcgcgcccc cagcccccgg gcccagcacc ccaaggcggc
    14341 caacgccaaa actctccctc ctcctcttcc tcaatctcgc tctcgctctt tttttttttc
    14401 gcaaaaggag gggagagggg gtaaaaaaat gctgcactgt gcggcgaagc cggtgagtga
    14461 gcggcgcggg gccaatcagc gtgcgccgtt ccgaaagttg ccttttatgg ctcgagcggc
    14521 cgcggcggcg ccctataaaa cccagcggcg cgacgcgcca ccaccgccga gaccgcgtcc
    14581 gccccgcgag cacagagcct cgcctttgcc gatcctctag agtcgagatc cgccgccacc
    14641 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
    14701 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
    14761 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
    14821 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
    14881 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
    14941 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
    15001 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
    15061 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
    15121 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
    15181 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
    15241 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
    15301 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
    15361 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
    15421 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
    15481 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    15541 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    15601 cccacccccc ggatctaagc tctagataag taatgatcat aatcagccat atcacatctg
    15661 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    15721 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    15781 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    15841 ccaaactcat caatgtatct tatcatgtct ggatccgggg gtaccgcgtc gagaagttcc
    15901 tattccgaag ttcctattct ctagaaagta taggaacttc gtcgagataa cttcgtatag
    15961 catacattat acgaagttat gtcgagatat ctagacccag ctttcttgta caaagtggtt
    16021 gatatctcta tagtcgcagt aggcggatta aatatcacac aatcattaca caatcacctt
    16081 tcaagcctga catgaaagtg gttcttacta ctcagcccat cattgtcaac cagctgctgt
    16141 cattatcccc atgataaaag taaagaaggg aaggccagag aaacccaagt catagggcta
    16201 gcaagttgca agaccaagac tagacccaag gcctgtcaga ttccaaagcc agagctctac
    16261 tgatccctca tgccagggcc cagcaggaca gcccagggaa ccaagttaca ggtagctgag
    16321 ggctgacatc tgaagttctc attcatccag acacaagacc tagatttccc aataatctcc
    16381 agagctgtta gctcaggaat cactttttgt tccagggctt gtattcatgc caggaacgat
    16441 ccaactgacc agaaaagaca tcctctaccg gctgcaagca tccaaggcca agtgcattgt
    16501 ggctagtgag gaagtggccc ccgcagtgga ctccattgta tcagagtgtc ccagcctgaa
    16561 gactaaactc ctggtgtctc cacaccactg ggatggatgg ctcaactttc aggagttact
    16621 tcagtgagta tttgggtggg aggcactagc aacagcacaa atcacaggta cagctctgaa
    16681 tctgtccgtc caaagactca gcaatagcca gagttgatac cgggctcctt cccaaaacag
    16741 aatgttttta agagtatgag cttatgagta catgtgtgag atggcgcaac gcaattaatg
    16801 ataacttcgt atagcataca ttatacgaag ttatggtctg agctcgccat cagttcagaa
    16861 catatgtcct ctgtaataat aataggtact cttagaccct agaccatctc tccagctccc
    16921 taaaaagtca tttaatgaaa ttatttacag acagaagaaa tatgtacagc tctactctga
    16981 cttttctgtc tcctctgtgg cctatgatga gtgtgctata gagatgggcc tttgggacac
    17041 aactatcatg atctctcttt tactctttga acatgctcta gctcagaacc ttggcatcca
    17101 ctgtctctcc tgccccgatg ttctttccta gtcctttcta tagttgttct atcttgtcag
    17161 gtttcagttc aaatgtttta tcctacgtga tgctgctcct gaccatcttg atggaagccg
    17221 taaggctttt ttctccatgc atggactgtt tcttcgcgtt gctaggtcct ctgagtgatc
    17281 agactagcct cgtgttcttt tagattcagt ttttctactc caaggtttgg actggaaaat
    17341 taggctccaa agccaaatcg ggtccaccac ttgcttttgt aaataaagtg tcattggcac
    17401 acagaggtgc tcatttgttt gtatattgtc tatgtctgct ttcatttaga aatggtatgc
    17461 ctgctatctg gccctttgca gaaagcatga cagccgctgc cttaagttaa actctctaag
    17521 tatgccaagg gttttcttgt gtgttcgggt gataccttta tcttcaacac ataacaaaac
    17581 acagctgggt gctgaaggaa tagttaataa gcagacaaaa aaaaaaaaaa cagatcactt
    17641 aacctctgtt ttcttcatat gtaaaatggg gataactata ctgcaagtat ctagtgacat
    17701 ttgacagcaa aataagctaa tacatgagaa agaatttccc atgttatttt aaaaagtgag
    17761 aaaatgttca ttgacagcat aatccttatt aaaatagcag atgttttata aaaataaaaa
    17821 aaatccagct tatttttttt gtaagttata aactcaggta agctatgtga catttctgaa
    17881 aatctgcatc ttaatctatg agatcaggat aatgacagta tggggttgca gaaggaacaa
    17941 acgggataaa gcaatgtgac atgctcggca taatggctag aacaaatggc acagtagttg
    18001 gaactgtgtt tgaggctgac tcacatggca gacaaaggag cagttgaggc ttggttgact
    18061 tttggttggg agataggtgg ctgggtactg tcattttgga gaagggtgga tgggtactgt
    18121 catttgagga aagatgagac cagtgataca gcttgtcagc acggtacttg gtactgactg
    18181 aatgacaaga ccttggtctc cactctcagt gctacaaaat gaaagctaaa tgtagacatg
    18241 gggtatattc agaaggtcaa agcaggagaa tgaagaactt agaagccagt ttaaggtaca
    18301 caacaagata ctatctaaaa agaaggaaaa taaaaataaa acaggcaggc aggcagacaa
    18361 gaaggaagga aggaagggag ggggacgggt acggggatgg agacggatgt agagcaggaa
    18421 agaaccagca gagactgcta taagatagta agcttagttt taggggctgt taagagcact
    18481 gactattctt ccagaggacc caggtttgag tcccagcacc cacatggcag cccacaacca
    18541 tctgtaagcc aagttctaaa tgttccaatg cccaattctg gccttcatgg gcactgcatt
    18601 tatggggtac acagacatac atgcaggaaa atcacacatt tttaaaaaga caataagcca
    18661 gtcatatgga gatcagggat ctaggaagat ccaggtgtct aacaagtgtg gaatatgtga
    18721 gtctcaggta taatgataaa aatcttgaag ttaccaggaa aaagttatct ctgaaaattc
    18781 agaactcaaa acttagggag ccaaaaagaa agaaaaccca gacttagcat caccccatga
    18841 atatctttct gcctctcaga gactccaatg tctttgtttt ttcagggaaa agtaaacaca
    18901 tggatgaagc cttgtgttag taggtcctct tacttaatgg agaaacacca aagacaagag
    18961 atgtccccta agctaggatg ttagataaag taaactaatc acactctccg gaacctaaaa
    19021 catccaacat cacccaatat ttgttctgaa agaaaaaaaa caaaactcaa tgatcttttc
    19081 tattttaagg aaacagagtt gctaaaaata tctcaatatt ttgagaatcc atttttggca
    19141 ttatggactg aggatgttta ttacctaatg atctctatct tagtgccatc tcgatcaatg
    19201 gcattcactc atcattacaa tcatctgaat gttagagatg tacttcagat gatagaattt
    19261 gcctagcatg tgtgcagctc cagacttctc tagagccttg taacttgaat gtggtggtac
    19321 aagcgtataa tctcagcacc caagaagtag cagcaagagg aagtttaaca tcatcctcag
    19381 atgcaatttt cagccagtct gggtcttatg agataatgtc tcaaaataaa atgataacat
    19441 aaagttcact gaaataaaaa cctatcactt ggaattttcc aaaatgagaa atttgaaaaa
    19501 tataatacat gaggcataca gatagatgta atacagagca caatgatgca taccattcat
    19561 cccagcatcc aggaggcaag gcaagcagat atcatcaatg agtttaaggc cagactggtc
    19621 tacacgatgt tccaggccag tcatgatcta taatgtctac cagaagaagt atgtcagttc
    19681 acctcatgtg gccttctttg tcacacaaga caatctgaat cccagaatcc ctgccacaaa
    19741 atgtacacca gaataagtca taagcctcac ccagatcctt cagtgtggga atggcatgtt
    19801 tgtccaggga tgggaggaat tggaaagctg tgtacaccaa aagtcttttt ctgtcaccta
    19861 agatacacac tttccacctt agtaatccaa gtacagatga aacatacagt actgaggggt
    19921 gtctaggtgg ttcgatgaaa caccaacccc atctttgtta tgatgccagt atgactagga
    19981 gaattccaga catgtacatt gtatacattc tatacatgga caggtatcat gggttgtgat
    20041 gaaagatgaa tgggatggga gacagacaat ggcatcctgt cttcattact cctttttttc
    20101 ccatgaaggt gtgtgtgtgt gtgtgtatgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
    20161 gtgttgccat gtgtgtaggt acatatatat gtaagagtac acatgtacat gtttgcatgc
    20221 aaaggcctga ggctgatgtc aggtgtcttc ctcgatctct ctgccttact cattgagtca
    20281 ggagctctcc tcaaacccag agcccatcaa tgtcactagt cttgtgctag ggaatcaaat
    20341 ctgacctttc aagcaaagga atcacgatgg gccaccacgg ctccctggca tttacttggg
    20401 tgctgggata caaactttgg tcctcatcta cagcccacat cactcttctt aaaacaagaa
    20461 aatgtctcct gtaggacttt atacctgttg actataattc tcccaagcct attcctaaac
    20521 caatcactgg aaagggaaaa aaccatcctg attggcttag cttaattgtg actaagtgag
    20581 aagcatgtgg ctacctagta cctgaacaaa acctgacttt tgttagttca gtaaaaggat
    20641 gggatggcta tttggtccgc tattagctgt ttctgtcaga gatactgaaa taatggacaa
    20701 taacaacaaa ggttttaagt ttgatctcaa tatatctggc ctttaaatct aaaggtcttg
    20761 ctctaatccc taaatcatga aatggtatca acttagaaag actcaaatct gtttttaatt
    20821 tttttatata aattacaaag acattgaaat aaagtatatg tgcttctgtt attgacatga
    20881 cgagttacct tggctgtctt tttggactac aaagaaatga gcttaaaaag gaggacttta
    20941 ccctgatctt tagagtccct ctaagattct tgggagtttc tgctcaggga agctctgcag
    21001 taggttcagt tcattattcc cccgtggtaa gtgggaccct agtagagaac aatcatacag
    21061 agtcaactga tctcccctca gtctatatct tggggttcat ctgcagatcg gcctctgaag
    21121 agcacaattg tgtggagacg ggaagtcaag aaccaatggc catttacttc accagtggga
    21181 ccacaggctc ccccaagatg gctcagcact cacagggcag ccttggcatt gggtataccc
    21241 tttgtggaag gtaggaaagg aaactatggt tttgttctta ttttcattgc agcctggcct
    21301 cacactgtag ttggctctgg actataactc actctgtacc cttgactggc ctcaaattca
    21361 tagcaattct cctgcctctc ttccctcccc cacaaaaata gttgaatttg caggagtgca
    21421 ccaccacact gcttctttgt tttctatgca gtctcccagg ctgccagtgt gggtgtccaa
    21481 ctgctgatat gacacctctc cttgaatgga tattaagata tctttaacgt agatccataa
    21541 aacagaactc tggatgcctc taccctaccc tagctaaatg gtaccattga agtagctgat
    21601 caagcaagaa tgtaggttta gtgcttgatt catagcactt ctgcctctct ctctctcctt
    21661 atttccaggt gaatttacct tcccaaaagg gtctttgaaa caagtctacc taaaactgcc
    21721 acattgctct gtttaatttt ccttctacct aaagttaaag cattcaatga ttactctcaa
    21781 tcatcaaccc aataaagtct agaataccca agggattgt
//