LOCUS       KO-first_condition_ready_31533_MGI:2681844        21759 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Acsm4 targeting project(s): 31533
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 42446
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     exon            4378..4621
                     /db_xref="ENSEMBL:ENSMUSE00000421258"
                     /rank="2"
                     /note="ENSMUSE00000421258"
     exon            5381..5591
                     /db_xref="ENSEMBL:ENSMUSE00000233168"
                     /rank="3"
                     /note="ENSMUSE00000233168"
     misc_feature    4215..8864
                     /note="5 arm"
     misc_feature    8892..8916
                     /note="B1 Gateway"
     misc_feature    8929..8976
                     /note="Frt"
     intron          8977..9851
                     /note="mouse En2 intron"
     misc_feature    9849..9851
                     /note="Splice Acceptor"
     exon            9852..10024
                     /note="mouse En2 exon"
     misc_feature    10036..10629
                     /note="ECMV IRES"
     gene            10630..13690
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13729..13972
                     /note="SV40 polyadenylation site"
     misc_feature    13976..14009
                     /note="loxP"
     promoter        14036..14555
                     /note="human beta actin promoter"
     gene            14581..15375
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15575..15816
                     /note="SV40 polyadenylation site"
     misc_feature    15833..15880
                     /note="Frt"
     misc_feature    15887..15920
                     /note="loxP"
     misc_feature    9866..9943
                     /note="FCHK std_gpr"
     misc_feature    15936..15956
                     /note="B2 Gateway"
     misc_feature    8865..15986
                     /note="Synthetic Cassette"
     exon            16356..16563
                     /db_xref="ENSEMBL:ENSMUSE00000400031"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000400031"
     misc_feature    15987..16717
                     /note="Critical Region"
     misc_feature    16741..16774
                     /note="Downstream LoxP"
     misc_feature    16718..16797
                     /note="synthetic loxP region"
     exon            21047..21190
                     /db_xref="ENSEMBL:ENSMUSE00000412236"
                     /rank="5"
                     /note="ENSMUSE00000412236"
     misc_feature    16798..21759
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggaattgtt agggctaagc tggtgacatg cagaattaag aaacattcag
     4261 tagtggggac actgatcagt ggctaatggt gacagaccta tgcgaggatg gagcgttctt
     4321 caggaatcca ctcccagtct tcagctaaca ctctcccctc ctctctcttc cctacaggca
     4381 tttcccactt gctaccagaa aataaagttt ttcaggaacc atgaaggttc tcctccactg
     4441 tcagagactt agattcatct ggcttgccaa gccagccggc cggcacttcc acagagattc
     4501 ccagctctgg gcacctctga cactggatga ctttgaagcc atcaatcgct gtgagaagcc
     4561 attacccaag aactttaact ttgcagcaga tgttctggac cagtggtccc taaaggagaa
     4621 ggtatcaaag agcagggccc cagtgttcct gttgctctca tctctgtctt ccaggtcctc
     4681 tcactgtgtt ctacctgtca agtttaaatc aattttttta tctcagtgag ggcaggaacc
     4741 tggatggctt catggagact cataagtaca agagcccata aacacctttc attccaatca
     4801 gtttctgtct tttgattctt ctcagaaaac ttagagtcta gggagatgaa ttcagattaa
     4861 ctcttccttg gtatatgctt atcccctctt taaaaagggc agaccccacc aagaggtgat
     4921 tctccaagac tgttctccag tgttccactg gagagaaaca tttctcccct aggcacactg
     4981 gggaagcagc tctggaggag tagcaataga cgtcctatct gccttctgtg ttagtcagtg
     5041 tgccatcact gtgacaaaac acctaagaca cacaatttca agtgaatgaa ggttgcttcg
     5101 gttcatgggt gcaacgatat cagcttcggt tcacttagcc ctgtggcagc cctgagcagc
     5161 acagggaaca tgcatggtga ggaagactgc ctgctcccct ccaggacagc tcagagaggt
     5221 ggaaggaatg agtgagagga agggcctagg gtctgatcgt tttcttcttt aagccctaac
     5281 gccacaggat aggggactat gccttttaac atgtgggtct tcaggtgaca gctctaaacc
     5341 ataatacatt atcttctctt tcccatgtgt tcctggccag tctggagaga ggccagccaa
     5401 cccagccctg tggtgggtga atgggaaagg agatgaggtg aagtggagct tccaagaact
     5461 gggttccttg tcccgaaagg ctgccaatgt gctcaccaag ccctgcggcc tgcagagagg
     5521 agaccgggtg gctgtgattc tgccccgtat ccctgaatgg tggctcatca atgtggcttg
     5581 catgagaaca ggtgagtatc acaaatgagt gtcaaaactt gagacttttg tgaagggtca
     5641 gctttgacta aagcaatcct tacaaaaagt agagaaagaa agttaacctt aatagcagtt
     5701 gtgatgaaat aataacaaca aacttaagaa attgggaaca ctgtgataac cattttttga
     5761 gagaggaaat caaagcacag tgatattaag acatttgcca aaggtcacat cacttgaaaa
     5821 ttatgtaatt ggggacctgg gaagatgact cagtggataa agtacttgct gtacaaacat
     5881 gtggccctgc attcccacat caaaagcttg gcatggaggg acaggtttct atgatcccag
     5941 cactgtgggc gcagagacag gcacatcccc tggtctcact ggccagccac acctagtcaa
     6001 gacagaaagt ctcaggttca cacacatata cccacacaca caaataaaaa tgaaaaagag
     6061 agttgcacta ttcagagttg cagaaaaaag ctctataaag caaatgcaag tgtgagtgtt
     6121 tctccacagc acagggtgtg tggaatatcc cccccataca cacacacaca cacacacaca
     6181 cacacacaca cacacacaca cgcgcgcaca cacgcgcgca cacacacaca cacgcacaca
     6241 cacggttctc tcttctgggt gtgtatctag attctctgca acacaaaaat acagaagaag
     6301 tggaagtctt tgaggatgca aagatttgta agaacataag ggactctgga cccaagtttg
     6361 gaaaaaaaaa aaaaactcca gtcagaagaa ctaatgtatc cagttacaca ttttccccaa
     6421 agtagtaaca tgtaaagtaa agacatgtct ttcagatagg ggtctgtgag ccatgaacta
     6481 tggttcctct gcttgggtaa atagacgtgt gatggaatac agctgactcc ttttgctcca
     6541 cagcaaacgt gctgactgag accaagaaca atcctacagg ccacagagtc taacacactc
     6601 tccaggcctt ttaccaaaag actctctgat ttcttatctt taaaaaaaaa aaatcatgcc
     6661 gggcatggtg gcacatgcct ttaattccag cactcaggag gcagaggcag tcagacttct
     6721 gagttcaagg ccagcctggt ctacaaagtg agttctagga cagccagggc tatacagaga
     6781 aaccctgcct cgaaaaacca acaaaaaatt aaaaaataaa aaaaatcatc agacatccat
     6841 taaaataaat aagaatgcca aagtgtagac tactacagag aacagaaaga tatctgtgca
     6901 atgttattag caggtataca aaaccaaact taaatagaaa tgattaaaca atgcataacc
     6961 tagctaaggt gtaagtttag ttgagttgtt gattgatggt tgaatggctt gatcttgatt
     7021 aaaaaataat ttatactagc aataataaca tcaggagaat ttcagagaat ttaagatgct
     7081 tttaatgcca ataaaataca tgaaagcagg agtataaaaa tgtttaaatt caccatataa
     7141 aaagacagag ataaattgga aatcagccgt ggttggaaga agattaagga tggtttatct
     7201 gttatgaaat atacccttaa tgtaatttaa agtaaattat ttaacttagg aaaatctgtg
     7261 agtacaaaca cacaagctag tgttcaaaaa catgaaataa aacaataaca acagttcatc
     7321 aagtgaagaa agccagctgc agacctgctt attccccaaa atgtcaaacc cttcatgtga
     7381 atttttaaaa ttgccttcac tcaccacata acaaactatt tgattttgct ggaaggcata
     7441 tgcaataaag tctaaaatac ttgccatcca acaaacattt gctgtctaaa acccattaga
     7501 agaaatcttc ctctgactcc caaggtggta atctactact gaacacattc ataaatccac
     7561 cacggagact gtaactggga atcattaaga gcaaataact tgtaagagaa atcattttat
     7621 atttttatat taattgatcg ttctgagact ctctaacgtg ttttcttaag ggaataactc
     7681 agctaactca gttaaaatat actcagtaaa atagccttgg gatggcaaga tggctctgca
     7741 ggtaaaggtg cttgctatgc aagtctggcc accagagctt ctgagtccat gagaccaacc
     7801 taaaggtgga aggagagcgt tgatattaca aaaggtgtcc tctgcccata tgcgtaccac
     7861 agcatgagag ctccccacaa taacactgat gacagtaaaa aaagagcctc cttcctagga
     7921 attcaactcc acaacttcct cctatttagc tgatttcaga atacccagca aggagtacat
     7981 acatagacat acaacacagg tcttgggagt gatagaaatg tctctgggga ctaaccttgc
     8041 tattggcatt gccatttatt ggctttgtga cccgtaggtg cgttatcatt cactggcatg
     8101 tgatatgtgc tctgtgcacg gcagtggcta tctgtaatgt tttgagtatc actgtcttcc
     8161 catggattca ctttagagag tcaaaccacc tgcttacatc ttatccttac atctaatcct
     8221 tacaccatac atgtaatcct gccctgtgca gtcttctcat aaggagcttt gaaatcctgt
     8281 ctctggctgc tgtttgggat gaagagggat aaattaagca tgaccatcgt catctaggaa
     8341 aatcttggcg gtaccagacc acggcatcaa tgatttgtag gctggctctg ccactcacca
     8401 gctatcccca aagactgcat gcacctcgtg gggatgctta aaacagtagt ttcaggttac
     8461 taaggtggct tggttactaa agatgcttgc tgcacgagcc tagctacatg agttagattc
     8521 ctaggaccca caaaaaggta caaggaaaga accacatcca caatggtatc atctgacctc
     8581 catgcaaaca acacacacac atacacacac acacacacac aaatgataga ttaaaaaaaa
     8641 tccttttaag tttttgaaga atagtctctg agcatctggg gctatgcaat ctatcaaaaa
     8701 aaattaatga gcaggcattt tcctagctgc atataatttt tcactctgtc ctgttgcact
     8761 gatgtagtat gcatcttaca aagcatttca acaaagagaa gaattctagt gtctctgtac
     8821 cttttacata tctattattt gcttttctca ttcccagata aattaaggcg cataacgata
     8881 ccacgatatc aacaagtttg tacaaaaaag caggctggcg ccggaaccga agttcctatt
     8941 ccgaagttcc tattctctag aaagtatagg aacttcgaac cctttcccac accaccctcc
     9001 acacttgccc caaacactgc caactatgta ggaggaaggg gttgggacta acagaagaac
     9061 ccgttgtggg gaagctgttg ggagggtcac tttatgttct tgcccaaggt cagttgggtg
     9121 gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa ggtgagaggg acaggccacc
     9181 aaggtcagcc ccccccccct atcccatagg agccaggtcc ctctcctgga caggaagact
     9241 gaaggggaga tgccagagac tcagtgaagc ctggggtacc ctattggagt ccttcaagga
     9301 aacaaacttg gcctcaccag gcctcagcct tggctcctcc tgggaactct actgcccttg
     9361 ggatcccctt gtagttgtgg gttacatagg aagggggacg ggattcccct tgactggcta
     9421 gcctactctt ttcttcagtc ttctccatct cctctcacct gtctctcgac cctttcccta
     9481 ggatagactt ggaaaaagat aaggggagaa aacaaatgca aacgaggcca gaaagatttt
     9541 ggctgggcat tccttccgct agcttttatt gggatcccct agtttgtgat aggcctttta
     9601 gctacatctg ccaatccatc tcattttcac acacacacac accactttcc ttctggtcag
     9661 tgggcacatg tccagcctca agtttatatc accaccccca atgcccaaca cttgtatggc
     9721 cttgggcggg tcatcccccc ccccaccccc agtatctgca acctcaagct agcttgggtg
     9781 cgttggttgt ggataagtag ctagactcca gcaaccagta acctctgccc tttctcctcc
     9841 atgacaacca ggtcccaggt cccgaaaacc aaagaagaag aaccctaaca aagaggacaa
     9901 gcggcctcgc acagccttca ctgctgagca gctccagagg ctcaaggctg agtttcagac
     9961 caacaggtac ctgacagagc agcggcgcca gagtctggca caggagctcg gtacccggaa
    10021 gatctggact ctagagaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
    10081 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
    10141 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
    10201 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
    10261 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
    10321 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
    10381 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
    10441 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
    10501 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac
    10561 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt
    10621 gccacaacca tggaagatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    10681 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    10741 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc
    10801 tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc
    10861 gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc
    10921 aacgtgacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt
    10981 tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt
    11041 atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac
    11101 ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa
    11161 aaccgcctcg cggtgatggt gctgcgctgg agtgacggca gttatctgga agatcaggat
    11221 atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa
    11281 atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag
    11341 gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg
    11401 cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag
    11461 cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg
    11521 agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg
    11581 ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg
    11641 ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct
    11701 ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag
    11761 aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg
    11821 tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg
    11881 ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg
    11941 cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa
    12001 tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc
    12061 cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg
    12121 atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa
    12181 aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg
    12241 atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt
    12301 ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac
    12361 ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc
    12421 tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa
    12481 caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa
    12541 tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag
    12601 ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa
    12661 ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg
    12721 caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt
    12781 ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc
    12841 accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag
    12901 tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc
    12961 gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt
    13021 gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg
    13081 ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg
    13141 tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt
    13201 ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg
    13261 attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg
    13321 ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca
    13381 ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg
    13441 cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc
    13501 tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc
    13561 acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg
    13621 tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt
    13681 caaaaataat aataaccggg caggggggat ctaagctcta gataagtaat gatcataatc
    13741 agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    13801 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    13861 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    13921 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccggaataac
    13981 ttcgtatagc atacattata cgaagttatg tttaaacggc gcgccccgga attcgccttc
    14041 tgcaggagcg tacagaaccc agggccctgg cacccgtgca gaccctggcc caccccacct
    14101 gggcgctcag tgcccaagag atgtccacac ctaggatgtc ccgcggtggg tggggggccc
    14161 gagagacggg caggccgggg gcaggcctgg ccatgcgggg ccgaaccggg cactgcccag
    14221 cgtgggcgcg ggggccacgg cgcgcgcccc cagcccccgg gcccagcacc ccaaggcggc
    14281 caacgccaaa actctccctc ctcctcttcc tcaatctcgc tctcgctctt tttttttttc
    14341 gcaaaaggag gggagagggg gtaaaaaaat gctgcactgt gcggcgaagc cggtgagtga
    14401 gcggcgcggg gccaatcagc gtgcgccgtt ccgaaagttg ccttttatgg ctcgagcggc
    14461 cgcggcggcg ccctataaaa cccagcggcg cgacgcgcca ccaccgccga gaccgcgtcc
    14521 gccccgcgag cacagagcct cgcctttgcc gatcctctag agtcgagatc cgccgccacc
    14581 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
    14641 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
    14701 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
    14761 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
    14821 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
    14881 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
    14941 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
    15001 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
    15061 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
    15121 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
    15181 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
    15241 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
    15301 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
    15361 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
    15421 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    15481 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    15541 cccacccccc ggatctaagc tctagataag taatgatcat aatcagccat atcacatctg
    15601 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    15661 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    15721 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    15781 ccaaactcat caatgtatct tatcatgtct ggatccgggg gtaccgcgtc gagaagttcc
    15841 tattccgaag ttcctattct ctagaaagta taggaacttc gtcgagataa cttcgtatag
    15901 catacattat acgaagttat gtcgagatat ctagacccag ctttcttgta caaagtggtt
    15961 gatatctcta tagtcgcagt aggcggatta aatatcacac aatcattaca caatcacctt
    16021 tcaagcctga catgaaagtg gttcttacta ctcagcccat cattgtcaac cagctgctgt
    16081 cattatcccc atgataaaag taaagaaggg aaggccagag aaacccaagt catagggcta
    16141 gcaagttgca agaccaagac tagacccaag gcctgtcaga ttccaaagcc agagctctac
    16201 tgatccctca tgccagggcc cagcaggaca gcccagggaa ccaagttaca ggtagctgag
    16261 ggctgacatc tgaagttctc attcatccag acacaagacc tagatttccc aataatctcc
    16321 agagctgtta gctcaggaat cactttttgt tccagggctt gtattcatgc caggaacgat
    16381 ccaactgacc agaaaagaca tcctctaccg gctgcaagca tccaaggcca agtgcattgt
    16441 ggctagtgag gaagtggccc ccgcagtgga ctccattgta tcagagtgtc ccagcctgaa
    16501 gactaaactc ctggtgtctc cacaccactg ggatggatgg ctcaactttc aggagttact
    16561 tcagtgagta tttgggtggg aggcactagc aacagcacaa atcacaggta cagctctgaa
    16621 tctgtccgtc caaagactca gcaatagcca gagttgatac cgggctcctt cccaaaacag
    16681 aatgttttta agagtatgag cttatgagta catgtgtgag atggcgcaac gcaattaatg
    16741 ataacttcgt atagcataca ttatacgaag ttatggtctg agctcgccat cagttcagaa
    16801 catatgtcct ctgtaataat aataggtact cttagaccct agaccatctc tccagctccc
    16861 taaaaagtca tttaatgaaa ttatttacag acagaagaaa tatgtacagc tctactctga
    16921 cttttctgtc tcctctgtgg cctatgatga gtgtgctata gagatgggcc tttgggacac
    16981 aactatcatg atctctcttt tactctttga acatgctcta gctcagaacc ttggcatcca
    17041 ctgtctctcc tgccccgatg ttctttccta gtcctttcta tagttgttct atcttgtcag
    17101 gtttcagttc aaatgtttta tcctacgtga tgctgctcct gaccatcttg atggaagccg
    17161 taaggctttt ttctccatgc atggactgtt tcttcgcgtt gctaggtcct ctgagtgatc
    17221 agactagcct cgtgttcttt tagattcagt ttttctactc caaggtttgg actggaaaat
    17281 taggctccaa agccaaatcg ggtccaccac ttgcttttgt aaataaagtg tcattggcac
    17341 acagaggtgc tcatttgttt gtatattgtc tatgtctgct ttcatttaga aatggtatgc
    17401 ctgctatctg gccctttgca gaaagcatga cagccgctgc cttaagttaa actctctaag
    17461 tatgccaagg gttttcttgt gtgttcgggt gataccttta tcttcaacac ataacaaaac
    17521 acagctgggt gctgaaggaa tagttaataa gcagacaaaa aaaaaaaaaa cagatcactt
    17581 aacctctgtt ttcttcatat gtaaaatggg gataactata ctgcaagtat ctagtgacat
    17641 ttgacagcaa aataagctaa tacatgagaa agaatttccc atgttatttt aaaaagtgag
    17701 aaaatgttca ttgacagcat aatccttatt aaaatagcag atgttttata aaaataaaaa
    17761 aaatccagct tatttttttt gtaagttata aactcaggta agctatgtga catttctgaa
    17821 aatctgcatc ttaatctatg agatcaggat aatgacagta tggggttgca gaaggaacaa
    17881 acgggataaa gcaatgtgac atgctcggca taatggctag aacaaatggc acagtagttg
    17941 gaactgtgtt tgaggctgac tcacatggca gacaaaggag cagttgaggc ttggttgact
    18001 tttggttggg agataggtgg ctgggtactg tcattttgga gaagggtgga tgggtactgt
    18061 catttgagga aagatgagac cagtgataca gcttgtcagc acggtacttg gtactgactg
    18121 aatgacaaga ccttggtctc cactctcagt gctacaaaat gaaagctaaa tgtagacatg
    18181 gggtatattc agaaggtcaa agcaggagaa tgaagaactt agaagccagt ttaaggtaca
    18241 caacaagata ctatctaaaa agaaggaaaa taaaaataaa acaggcaggc aggcagacaa
    18301 gaaggaagga aggaagggag ggggacgggt acggggatgg agacggatgt agagcaggaa
    18361 agaaccagca gagactgcta taagatagta agcttagttt taggggctgt taagagcact
    18421 gactattctt ccagaggacc caggtttgag tcccagcacc cacatggcag cccacaacca
    18481 tctgtaagcc aagttctaaa tgttccaatg cccaattctg gccttcatgg gcactgcatt
    18541 tatggggtac acagacatac atgcaggaaa atcacacatt tttaaaaaga caataagcca
    18601 gtcatatgga gatcagggat ctaggaagat ccaggtgtct aacaagtgtg gaatatgtga
    18661 gtctcaggta taatgataaa aatcttgaag ttaccaggaa aaagttatct ctgaaaattc
    18721 agaactcaaa acttagggag ccaaaaagaa agaaaaccca gacttagcat caccccatga
    18781 atatctttct gcctctcaga gactccaatg tctttgtttt ttcagggaaa agtaaacaca
    18841 tggatgaagc cttgtgttag taggtcctct tacttaatgg agaaacacca aagacaagag
    18901 atgtccccta agctaggatg ttagataaag taaactaatc acactctccg gaacctaaaa
    18961 catccaacat cacccaatat ttgttctgaa agaaaaaaaa caaaactcaa tgatcttttc
    19021 tattttaagg aaacagagtt gctaaaaata tctcaatatt ttgagaatcc atttttggca
    19081 ttatggactg aggatgttta ttacctaatg atctctatct tagtgccatc tcgatcaatg
    19141 gcattcactc atcattacaa tcatctgaat gttagagatg tacttcagat gatagaattt
    19201 gcctagcatg tgtgcagctc cagacttctc tagagccttg taacttgaat gtggtggtac
    19261 aagcgtataa tctcagcacc caagaagtag cagcaagagg aagtttaaca tcatcctcag
    19321 atgcaatttt cagccagtct gggtcttatg agataatgtc tcaaaataaa atgataacat
    19381 aaagttcact gaaataaaaa cctatcactt ggaattttcc aaaatgagaa atttgaaaaa
    19441 tataatacat gaggcataca gatagatgta atacagagca caatgatgca taccattcat
    19501 cccagcatcc aggaggcaag gcaagcagat atcatcaatg agtttaaggc cagactggtc
    19561 tacacgatgt tccaggccag tcatgatcta taatgtctac cagaagaagt atgtcagttc
    19621 acctcatgtg gccttctttg tcacacaaga caatctgaat cccagaatcc ctgccacaaa
    19681 atgtacacca gaataagtca taagcctcac ccagatcctt cagtgtggga atggcatgtt
    19741 tgtccaggga tgggaggaat tggaaagctg tgtacaccaa aagtcttttt ctgtcaccta
    19801 agatacacac tttccacctt agtaatccaa gtacagatga aacatacagt actgaggggt
    19861 gtctaggtgg ttcgatgaaa caccaacccc atctttgtta tgatgccagt atgactagga
    19921 gaattccaga catgtacatt gtatacattc tatacatgga caggtatcat gggttgtgat
    19981 gaaagatgaa tgggatggga gacagacaat ggcatcctgt cttcattact cctttttttc
    20041 ccatgaaggt gtgtgtgtgt gtgtgtatgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
    20101 gtgttgccat gtgtgtaggt acatatatat gtaagagtac acatgtacat gtttgcatgc
    20161 aaaggcctga ggctgatgtc aggtgtcttc ctcgatctct ctgccttact cattgagtca
    20221 ggagctctcc tcaaacccag agcccatcaa tgtcactagt cttgtgctag ggaatcaaat
    20281 ctgacctttc aagcaaagga atcacgatgg gccaccacgg ctccctggca tttacttggg
    20341 tgctgggata caaactttgg tcctcatcta cagcccacat cactcttctt aaaacaagaa
    20401 aatgtctcct gtaggacttt atacctgttg actataattc tcccaagcct attcctaaac
    20461 caatcactgg aaagggaaaa aaccatcctg attggcttag cttaattgtg actaagtgag
    20521 aagcatgtgg ctacctagta cctgaacaaa acctgacttt tgttagttca gtaaaaggat
    20581 gggatggcta tttggtccgc tattagctgt ttctgtcaga gatactgaaa taatggacaa
    20641 taacaacaaa ggttttaagt ttgatctcaa tatatctggc ctttaaatct aaaggtcttg
    20701 ctctaatccc taaatcatga aatggtatca acttagaaag actcaaatct gtttttaatt
    20761 tttttatata aattacaaag acattgaaat aaagtatatg tgcttctgtt attgacatga
    20821 cgagttacct tggctgtctt tttggactac aaagaaatga gcttaaaaag gaggacttta
    20881 ccctgatctt tagagtccct ctaagattct tgggagtttc tgctcaggga agctctgcag
    20941 taggttcagt tcattattcc cccgtggtaa gtgggaccct agtagagaac aatcatacag
    21001 agtcaactga tctcccctca gtctatatct tggggttcat ctgcagatcg gcctctgaag
    21061 agcacaattg tgtggagacg ggaagtcaag aaccaatggc catttacttc accagtggga
    21121 ccacaggctc ccccaagatg gctcagcact cacagggcag ccttggcatt gggtataccc
    21181 tttgtggaag gtaggaaagg aaactatggt tttgttctta ttttcattgc agcctggcct
    21241 cacactgtag ttggctctgg actataactc actctgtacc cttgactggc ctcaaattca
    21301 tagcaattct cctgcctctc ttccctcccc cacaaaaata gttgaatttg caggagtgca
    21361 ccaccacact gcttctttgt tttctatgca gtctcccagg ctgccagtgt gggtgtccaa
    21421 ctgctgatat gacacctctc cttgaatgga tattaagata tctttaacgt agatccataa
    21481 aacagaactc tggatgcctc taccctaccc tagctaaatg gtaccattga agtagctgat
    21541 caagcaagaa tgtaggttta gtgcttgatt catagcactt ctgcctctct ctctctcctt
    21601 atttccaggt gaatttacct tcccaaaagg gtctttgaaa caagtctacc taaaactgcc
    21661 acattgctct gtttaatttt ccttctacct aaagttaaag cattcaatga ttactctcaa
    21721 tcatcaaccc aataaagtct agaataccca agggattgt
//