LOCUS       KO-first_condition_ready_23200_MGI:2442633        21548 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Rabl6 targeting project(s): 23200
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 34152
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            6422..6469
                     /db_xref="ENSEMBL:ENSMUSE00001233674"
                     /rank="3"
                     /note="ENSMUSE00001233674"
     exon            5265..5399
                     /db_xref="ENSEMBL:ENSMUSE00001240326"
                     /rank="2"
                     /note="ENSMUSE00001240326"
     misc_feature    4275..9164
                     /note="5 arm"
     misc_feature    9192..9216
                     /note="B1 Gateway"
     misc_feature    9229..9276
                     /note="Frt"
     intron          9277..10151
                     /note="mouse En2 intron"
     misc_feature    10149..10151
                     /note="Splice Acceptor"
     exon            10152..10324
                     /note="mouse En2 exon"
     misc_feature    10336..10929
                     /note="ECMV IRES"
     gene            10930..13990
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14029..14272
                     /note="SV40 polyadenylation site"
     misc_feature    14276..14309
                     /note="loxP"
     promoter        14336..14855
                     /note="human beta actin promoter"
     gene            14881..15675
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15875..16116
                     /note="SV40 polyadenylation site"
     misc_feature    16133..16180
                     /note="Frt"
     misc_feature    16187..16220
                     /note="loxP"
     misc_feature    10166..10243
                     /note="FCHK std_gpr"
     misc_feature    16236..16256
                     /note="B2 Gateway"
     misc_feature    9165..16286
                     /note="Synthetic Cassette"
     exon            16706..16758
                     /db_xref="ENSEMBL:ENSMUSE00001277210"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00001277210"
     misc_feature    16287..16914
                     /note="Critical Region"
     misc_feature    16938..16971
                     /note="Downstream LoxP"
     misc_feature    16915..16994
                     /note="synthetic loxP region"
     exon            18370..18461
                     /db_xref="ENSEMBL:ENSMUSE00001304516"
                     /rank="5"
                     /note="ENSMUSE00001304516"
     misc_feature    16995..21548
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggaacacct cctaatcttc ctaagcagtc aactaagcca actgtttgac
     4321 cactcactca tatgtgcact gggggccatt ctcagccaga ctttggtttg gaaatgggga
     4381 gaggcttgac tgcaggcaga ccagacatcc taggagaaag cagcaggtgg gtaaaggaac
     4441 ccacaagctt cccctcgccc acccccccgc cctggaagcc tccatcgctg ctttagactc
     4501 tcgagggtgt tttcggtggc atgattagcc tctgcctgtc tcatggtgct gcccaggaag
     4561 taccatgcta acacccatgc cttccctgcc catctcatca tcctctgtct gtctgtctgt
     4621 ctgtcctcag ctttgctctc gtccctcctg gcttcgctga cactgggtca gctccagctt
     4681 tttgttacct gtgggaacac ctagtggtaa acttctctaa gaggtctcgg acctaaggtc
     4741 gacactccat cctgttcacc ccattcctgg aacacatgcc cgtccctgcc caaagtcagg
     4801 agaacctgct gagttcaggc ctagtacacc atggagtgag tgaattagga tacagacatg
     4861 cggaactaca ccacaagctt gaggactgag aaatgtcagc aggaagccat gtcctgggta
     4921 ccatcctggg ccctacacac tgttggctca agtctgcagc ccagctctga tcctagggcc
     4981 cctggcagtt cctcagagta tataactcca ggagacccag gccctgatga agtacttcag
     5041 tgctgcctcc tctaccagcc ctggcttctg ttcttgccaa gtctgccaag ccggaattga
     5101 cccgctgggg aactgctggt atcctcacgc agtcctcccc tgcatgccac tcgcctgaga
     5161 aaatagggat ccaccttgac atggaacaga tgggatactt taaagttttt taaaaatgac
     5221 tggttaatct tcccttctct tttctttgcc catccttgcc ccagtgaaga ttgtgatccg
     5281 gggtgacagg aacacgggca agacagctct gtggcatcga ctgcagggca agaagtttgt
     5341 ggaagaatac atccccacac aggagatcca ggtcaccagc atccattgga actacaaaag
     5401 tgagttcaag gccaataata gttcaatgcc acttttcccc tgtacagtat agtagtatcc
     5461 tgccttcctg gctcctagaa ggtactttct gaaactgaga aaatactacc catgtgagag
     5521 gccccattaa ttaaggttgc atgtgaatca gcccttctgg gttttgaatc tgacatttct
     5581 cctctgtggt gcctcaggcc caggatactc atggttacaa gtaaggaagc aaggacatac
     5641 cttcaaagac tagaattccc tggccacctc taggagcaga tccacccata cacatcagca
     5701 acatacatgg ctgctttgtc tgataggaat gcattctgtg atccatggca agtgctcacc
     5761 cctggggaac agagtgacaa ttttaactct tgtcttcccc ttctgtgttc tggggtctcc
     5821 accatcctta cagccccatt agcccttagg acatcctaaa aatggagaat tgctactgag
     5881 gaggggttga agagttaaca agaacttgga aaatggtttc tgttctcatg caacttaagt
     5941 ctagtatcag agaaagcctt aggagtctat gggccaggta ggctgaggac ggtgatgtag
     6001 gtgtcggggc tacaactgac ctgggcctgc tcaggtgacc catcacagct ctggcctttg
     6061 caactgacct gcgttctcac tgagtactgg ccagtcaggt aaaggagtag taggcttggg
     6121 ggagatgtga cttggacagc tgtgctgttt cctcatctgc ccagagagtg agcatccgct
     6181 agggcacctg aagtggccca gggacccaca ggcttctgag tgtgagttgt tatgtgatgg
     6241 ctttgtctgt gggctaatat atgcattgct catgccaggg cctggaggaa tagccctgaa
     6301 aaccctacag catgtattgc agaggcccca gggagctagt aataccaaat gcccctggcc
     6361 ccatttggga tcctagaagg aatgatacaa tgcactaatt tgagagtttt tcttgtttca
     6421 gctactgatg atgtagtgaa agttgaagtc tgggatgtag ttgacaaagg taagatacat
     6481 tatctcctta atgcatgata gactctaagg cccctcccct ttgcacacac cccttatttg
     6541 catgtccttg ggtcccatgt cctacttagc tctgtcacac agagtcactt gtggctcaga
     6601 agcatgtcac aggcaccagc tttatcaagt caaaagagta taggagggtt gggtttgctg
     6661 ccaactgtta gcatctggct tacatcagag ccctatctga tcccttccct acgtggacta
     6721 agttctgggc cctggtcact tccatcccat gtctggtcgg cagagtaaat gttcaacagt
     6781 gaaactgtca cacatacttt tctcttttga gcattttggg agggatgacc cttatgaagc
     6841 gctgttccca aagccctcct gtgtatttcc tcttgtgtca gccctgcacc cacagtcaca
     6901 ctgagcccta gagtcttagt ggcttggcag ccctgctctg agagagacag aagggtacct
     6961 ctgtcagttg ttcttttact aggtgatggc ctaatgccat gttgtttgac agtgaccagt
     7021 gagtggtctc taatgagaat ttgttttcac aggcagatgc ccacaaggct cttacctgac
     7081 ttgggtcctg tttatagcta cctgcttagc cctctggcac tggaggtggc cctaattatt
     7141 gagctagaca gtgtaagtgc agtgcaccag cacagtagaa gtgtgcaccc tgagtgcgga
     7201 aggccaagtc agtggagcga gagcacactg gggcagcacg tggggcgtgc aggacttcgt
     7261 tcagagattg cccacaagca gttttaattg gatttagaaa gaaggcaagc tggagttgca
     7321 tgttcttact tgaacccatc tctgtcatgc atgtggtgga ggtcagcaac ctctatgtag
     7381 cgtttcttac tgaatgggtc cccaagtcag agtacctcga tagacccagc cacacagaga
     7441 ccagcctcat ctaaacctgc agaggtcaac agcagaactc ctcagagtgg ccctaatgac
     7501 aggatctgat ctcattcttc ctatgttagg tcttatgttc ctctttgacc ctgggggtag
     7561 gagttcatag tcctccttgg tgctgacatt gtatgtgtgc atgcaggcct ctgacccaac
     7621 aggcatagca tcacctattt agccactagc ataataaaca tgacctctga cctcttacaa
     7681 ccactggcct catcagcata gctgtgctgc atgtcctcca ggtacaggac ttggccagaa
     7741 gatgtgtctt cagggctccg ttttttggtt tttttgtttt ggcacagggt gtgctttctt
     7801 ccccctcctc agccaaatcc cttctgagta tggttttggg ccaagtgttg aagaacagtt
     7861 gaagccagtt ttttctctga ttccagcagt aatacagcct agttgaatcc tatccattga
     7921 gcctctctga tggctttggt gtgtctgtac acctctgaat gcttttgtgt gcatctttgg
     7981 agtattaata gatgacctgc tacctgaagt ccatcaatgt atacactgag ggcaagagtc
     8041 acatcctcat gaggccatca agtgacttac gccagctgtt ctccacaaac catgacccca
     8101 ttaagtcagg ctgtggacct gctgcagcgc cttcctaagt tcttgcctca gctgtggtct
     8161 ccacgtctgc agtctgctgc agcggatgca ctgtcctata gacagggctt tgaagagtgg
     8221 ggtcagaact tggggctgga tatcagatgg gacaataatg cagtagcctc agtctctcac
     8281 tcagggctct ttggagtagc attttacacc ctcagctggc ccaggaaccg tgcagcagag
     8341 gcgacacctc cactgcccag gagccatgga agactgattt cctgctgtgc taggctcaca
     8401 cgccctctac caccctactc tgagatctct gctcagatcc ctgctcagat gcagtcaggc
     8461 tgaatcccag cacagaggtc ctaactctgg gagtgcctgc atggtcacgc ctgctgctac
     8521 ctcttcaccg tagagggttg gaaagaaagg acctctgcct ctccttcctc ccccatcccc
     8581 atgatcttga atagtgtcca cagcaagcaa aggaccttta gatgtgagag aagttaaagc
     8641 aactaagggc tcaagggcat tggaaagttc tccagagaac tggccaagca gttgagctca
     8701 gcagctcaag ctttgtggag gcagagctct ctagtgcttg ttcagaagct ttcaaaggga
     8761 aagcatattt cccacaaacc ctttacagca gcttacagca agatgctggt gggctagagg
     8821 ctcatgccct gatgtgccac ttgccattca tcccatgatt caggctgaat catcactgcc
     8881 ctgagcagag aggctgacct gactgagtgt gacgggggct caggaaggac tgcagagtgc
     8941 tgtggaggtt ctgtgtaagc acatgaacct gagtttgttc tcaggagata atttgtctgc
     9001 tatggaattc ccaaggttgt gaagagctca gttggacatt tagatgaaaa ggagaaggga
     9061 cagtgatgtc atcttgttgt ccctagagtc cagtgtcaga ctgctacagg acctgtggtt
     9121 gtctgccaga taaaaacact tcagttacca tgaacaggca gtgtaaggcg cataacgata
     9181 ccacgatatc aacaagtttg tacaaaaaag caggctggcg ccggaaccga agttcctatt
     9241 ccgaagttcc tattctctag aaagtatagg aacttcgaac cctttcccac accaccctcc
     9301 acacttgccc caaacactgc caactatgta ggaggaaggg gttgggacta acagaagaac
     9361 ccgttgtggg gaagctgttg ggagggtcac tttatgttct tgcccaaggt cagttgggtg
     9421 gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa ggtgagaggg acaggccacc
     9481 aaggtcagcc ccccccccct atcccatagg agccaggtcc ctctcctgga caggaagact
     9541 gaaggggaga tgccagagac tcagtgaagc ctggggtacc ctattggagt ccttcaagga
     9601 aacaaacttg gcctcaccag gcctcagcct tggctcctcc tgggaactct actgcccttg
     9661 ggatcccctt gtagttgtgg gttacatagg aagggggacg ggattcccct tgactggcta
     9721 gcctactctt ttcttcagtc ttctccatct cctctcacct gtctctcgac cctttcccta
     9781 ggatagactt ggaaaaagat aaggggagaa aacaaatgca aacgaggcca gaaagatttt
     9841 ggctgggcat tccttccgct agcttttatt gggatcccct agtttgtgat aggcctttta
     9901 gctacatctg ccaatccatc tcattttcac acacacacac accactttcc ttctggtcag
     9961 tgggcacatg tccagcctca agtttatatc accaccccca atgcccaaca cttgtatggc
    10021 cttgggcggg tcatcccccc ccccaccccc agtatctgca acctcaagct agcttgggtg
    10081 cgttggttgt ggataagtag ctagactcca gcaaccagta acctctgccc tttctcctcc
    10141 atgacaacca ggtcccaggt cccgaaaacc aaagaagaag aaccctaaca aagaggacaa
    10201 gcggcctcgc acagccttca ctgctgagca gctccagagg ctcaaggctg agtttcagac
    10261 caacaggtac ctgacagagc agcggcgcca gagtctggca caggagctcg gtacccggaa
    10321 gatctggact ctagagaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
    10381 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
    10441 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
    10501 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
    10561 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
    10621 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
    10681 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
    10741 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
    10801 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac
    10861 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt
    10921 gccacaacca tggaagatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    10981 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    11041 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc
    11101 tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc
    11161 gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc
    11221 aacgtgacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt
    11281 tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt
    11341 atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac
    11401 ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa
    11461 aaccgcctcg cggtgatggt gctgcgctgg agtgacggca gttatctgga agatcaggat
    11521 atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa
    11581 atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag
    11641 gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg
    11701 cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag
    11761 cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg
    11821 agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg
    11881 ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg
    11941 ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct
    12001 ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag
    12061 aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg
    12121 tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg
    12181 ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg
    12241 cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa
    12301 tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc
    12361 cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg
    12421 atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa
    12481 aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg
    12541 atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt
    12601 ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac
    12661 ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc
    12721 tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa
    12781 caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa
    12841 tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag
    12901 ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa
    12961 ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg
    13021 caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt
    13081 ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc
    13141 accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag
    13201 tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc
    13261 gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt
    13321 gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg
    13381 ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg
    13441 tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt
    13501 ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg
    13561 attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg
    13621 ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca
    13681 ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg
    13741 cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc
    13801 tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc
    13861 acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg
    13921 tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt
    13981 caaaaataat aataaccggg caggggggat ctaagctcta gataagtaat gatcataatc
    14041 agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    14101 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    14161 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    14221 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccggaataac
    14281 ttcgtatagc atacattata cgaagttatg tttaaacggc gcgccccgga attcgccttc
    14341 tgcaggagcg tacagaaccc agggccctgg cacccgtgca gaccctggcc caccccacct
    14401 gggcgctcag tgcccaagag atgtccacac ctaggatgtc ccgcggtggg tggggggccc
    14461 gagagacggg caggccgggg gcaggcctgg ccatgcgggg ccgaaccggg cactgcccag
    14521 cgtgggcgcg ggggccacgg cgcgcgcccc cagcccccgg gcccagcacc ccaaggcggc
    14581 caacgccaaa actctccctc ctcctcttcc tcaatctcgc tctcgctctt tttttttttc
    14641 gcaaaaggag gggagagggg gtaaaaaaat gctgcactgt gcggcgaagc cggtgagtga
    14701 gcggcgcggg gccaatcagc gtgcgccgtt ccgaaagttg ccttttatgg ctcgagcggc
    14761 cgcggcggcg ccctataaaa cccagcggcg cgacgcgcca ccaccgccga gaccgcgtcc
    14821 gccccgcgag cacagagcct cgcctttgcc gatcctctag agtcgagatc cgccgccacc
    14881 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
    14941 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
    15001 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
    15061 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
    15121 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
    15181 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
    15241 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
    15301 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
    15361 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
    15421 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
    15481 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
    15541 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
    15601 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
    15661 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
    15721 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    15781 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    15841 cccacccccc ggatctaagc tctagataag taatgatcat aatcagccat atcacatctg
    15901 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    15961 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    16021 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    16081 ccaaactcat caatgtatct tatcatgtct ggatccgggg gtaccgcgtc gagaagttcc
    16141 tattccgaag ttcctattct ctagaaagta taggaacttc gtcgagataa cttcgtatag
    16201 catacattat acgaagttat gtcgagatat ctagacccag ctttcttgta caaagtggtt
    16261 gatatctcta tagtcgcagt aggcggagtg atgcagggac tgtttaagga actcagattt
    16321 ggtctccaag ctttgactgt agaaaccctt tatcatgcca tcccccacga ttccatgaga
    16381 gccaggctgt catgagcaac ccctttactc aaggcagacc tggataggga ctcttccttc
    16441 ccggcctgtc ttatcagctg ctaagtgctg catttcctat cctgctctgc cagcctagcc
    16501 agttcacaca gttttgtatc tctcaaacat gagtgagggg tgtctggacc taccaggagg
    16561 ctcttttaga ttgcaaggct gaatggagtg cagtcccacg tggcattaag gcggggagga
    16621 ggagtctgag gaaggatact ccccacttat gcaaatcctg ggcccaacca tttcattcct
    16681 aattttttgt ttgtctttta ataaggaaag tgtaaaaaac gaggagatgg cttgaagacg
    16741 gaaaatgacc cccaggaggt gagccacatt gtcaggtggg tctgtgccag tgtcatgagc
    16801 aaggcgcctg tgggcactct ctggcctcct ggacaatccc gtatgctgta gatgcagtag
    16861 tcactgactg tccttaacta ctgttgagac tcggtgctgc ctgggttaag tttcgagatg
    16921 gcgcaacgca attaatgata acttcgtata gcatacatta tacgaagtta tggtctgagc
    16981 tcgccatcag ttcaattagg aaccatattt tacatctctg tcatgtgact ttgttcttta
    17041 aagtgctgac atatgttgga ggtgtggcca tggcaccaca ggtgagacca ggtggtcttt
    17101 gcttgctccc tagaagctag aacacagtgg acatgtttgt gtaatccttc cttccagttg
    17161 ttttagagat ccctgccctg ccttctccct ctttgaggct ggaagtttaa gtgatacata
    17221 gccccttccc agcactgtcc catgtgctat gtgttcggga actcatcttg atggggttgg
    17281 gttttgtttt gtttcttttt accatttgca tagttttatt ttttttgtag atttattgac
    17341 agtttcattc acatatatta tatattctgt ttactcttta cctcctcttc tgtctccctc
    17401 ctaccctgtc atctgatccc tcttcctaca agtcctgtcc tctatgtgtg tctgtgtgtg
    17461 tcgggggcag aggacccagt gattttaaag taggaccctc tgtctgagca catgtttgga
    17521 attgtccatt gacatttgtt gtgctcactg ttgggtacag agctaggact gcaagagacc
    17581 tagcctccct agcctgtgat tgactgttga cagatgcagg ccaccacact aaccttagac
    17641 tctttctgct ctgtattcta ctgtgttctc tgagtctagt ttacagccat gcactaaatg
    17701 gtcatttatt cccaacactt tgagcaacca aaagaaaaga tttttttttt ttaatgaaag
    17761 tagcccttat atacagacag aaacataata ttaaatgcag tttggctaaa gaacagctgc
    17821 gcgcccactc accccacccc accccacccc accccagcct gtgactgccc agccctgggg
    17881 tgtgaccagg tttgttacac catgtgtcct tttttgttct tcccctggaa accatctcaa
    17941 ggggcagctg acagctccag cactttcttc tggaagtcct aaggcaggcc tgctctgtag
    18001 cgatggagct cactgctcca gcccccacgg ctaaccttgt gtatacccac aagggtttcc
    18061 tcaaggtttt tttcccaaaa tgaaatgact tttagataag aaaagcccca gccaggcagt
    18121 ggtggtgcat gcctttaatc tcagcacttg ggaggcagag gcaggcggat ttctgagttc
    18181 aaggccagcc tggtctacag agtgagttac acaacagcca gagctacaag agaaaccgtc
    18241 ttgaaaaaaa caaaacaaaa caaaaaaaga ctagaaaagc cccaacctgc atttttcagg
    18301 tgtttgaagg gccacaagta tgggattggc cagggacaca gtcttagcct ttcttccttc
    18361 tgtttctagg cagagtctga attggccctg gatgctgagt ttctggatgt gtacaagaac
    18421 tgtaatggag tggtcatgat gtttgacatc actaagcagt ggtaagaggg cagataacag
    18481 gcagtgctgc atgccttcat catgtagtgc tgtgcggttc ctctcagtgt ggaactgcct
    18541 actatgccag atgtgagcag aaccccctga cctagggggc tgaggtactg ataccacaag
    18601 acgggcagag tgtaggcagg agttggctca ggcttatagc caacagtggt agggatggag
    18661 cgtcaccttc tacaaccttg ctgtcctgaa aacaccatat aaaatttgtc agaatcaagt
    18721 aacctgttta attggagggg ctcccagaaa tacgcataca tatatataca cacactcagt
    18781 aactcaccaa gaagacatat ctaagatgag tgacaacaaa ggacaaagaa tgaagtgaga
    18841 aatgggtcca gaggttcagt gagcaggtgc aagcctcctg aaacccacca tactccattg
    18901 acaggggaca ttgtgagaaa ctgctttaca taccactgta agactgggaa ccctctttaa
    18961 ccaacagcag gaaaaccaag ggccccagag acttgcttcc tctgtgaaaa acagtctctt
    19021 aagaacacta tactagctaa acatggcggt gcacacctat taccccagaa catgggagac
    19081 agagacaggt gggtctcttg agttcaaagc ctacatagcg agttctaggc cagccagggc
    19141 tacatagtgc tattctgcct taaagaaaac ccactccatt acaagaagga aagttttact
    19201 ctgcttttac agttctatca gtattaatgt gtggaatctg tgttcagatt ggaaagagag
    19261 aaaagtgttg tgttccatca tgtaagactc tcattgcaga gagctgagca ggccagctag
    19321 atttctgtgc atcatctatg tacagtggaa ccatgtcttc ttaggctttg cacttgtgct
    19381 atttaagaat agtcacccac ttgattatac cagcctccaa catgtgcaca tcctaagcct
    19441 gggagtaaag tttgcactgt gcatgccact gattgtggtt tgagggtcct gaactcatag
    19501 atccttggga gccaccatct acataacata ctttctatag cctctgtaca gtattcatag
    19561 gcccagctcc aacctaccca accttcgggg acctatatgc ttaactgtca catgcaagaa
    19621 acctatgggg gctacaagag gcaggctagg ccataagctc cagttcagca gtacacttgt
    19681 gtcatagaca cccatataca aagctgttct tctcatgtac agagcagagt gcagtgctct
    19741 tgtgtacatg tacagtgtgt tttgccgtgt gggtgatggt agtctgttgg tctattatgt
    19801 gagacatggc ttctcccatg tgacattagg tttggacact gaaggcctct atactccact
    19861 gaagtgtgtt agaatctttg caatctaact tcacaagacc ctatagctat aagggggtat
    19921 tatagagggg tattatagag ggggtattat agaggggtag gtagtgctct taagaacagc
    19981 gaggacccaa aagacatgag ttctgggcta cagagtaggc aggaggctaa gccagctcag
    20041 gaaggatgca cagatgcata tttgagagcc atgaggcttc ataggtcctt gtcacaaaga
    20101 ccacaagtac cacagccatg tcttagggtc tgtcttagtc agggtttcta ttcctgcaca
    20161 aacatcatga ccaaaaagca agttggggag gaaagggttt attcagctta cacttccata
    20221 ctgctgttca tcaccaaagg aggtcaggac tggaactcaa gcaggtcagg gagcaggagc
    20281 tgatgcagag gccatggagg gatgttcttt actggcttgc ttcccctggc ttgctcagcc
    20341 tgctctctta aagaaccaag actaccagcc cagagatggc accacccaca aggggcctct
    20401 cccccttgat cgataattga gaaaatgccc cacagctgga tctcatggag gcatttcccc
    20461 aactgaagct ccattctctg tgaaacttca gttgtgtcaa gttgacacaa aactagccag
    20521 tacaattgac cccttgtcaa cttgacacac aaacacatca ctagaaagcc tcaaccctta
    20581 cattcttatt catccccaag atctaaataa ctttaaacgt cccacagtct ttacatattt
    20641 ttaaaatttc aatctcttta aaatatccat ctcttctaaa atccaaagtc tttttacaat
    20701 taaaagtctc ttaactgtgg gctccactaa aatagttgag cttgtcctgg gagtcagcag
    20761 acactgttaa gagtctgagc actgatcgtg aaggatggaa gcatccatgt tcacacagct
    20821 gctgccattg caagccagca agcacgcttc aagtgtctta taaggataaa aggggcagga
    20881 gatgtttcaa gcaaatgcag caaaaacaag agaagctgcc atagttaaaa acgtgttgcg
    20941 ttcttaattg cttggcaggg aagctgggtc atgctcattg cactgactgt gggaggtgtt
    21001 agtgattttt atagaaacta ggtctctgtg tagcttagtc taacctggaa cgactatact
    21061 tcccaaattg accacaaact cataatcctc ttacctgagc tttctgagta ctgtggttat
    21121 tggtatacac tgccactcat ggttggggag gaaggggagg ggagcagggc atgaggactg
    21181 gagagtgagc ctaggacctt gcatatgcta agcaagtgct cgacctctga ctatagccca
    21241 gtcctggctg gggcttcaca agccatttgg agacattggg atggcttata aagtgcaact
    21301 acagggcacc cccacattaa gtcagtcctg gagacctgga gactttgtag acagaatggt
    21361 ctaccctgca gaataaggaa tgtcctagag acaaacgcgt cactaaggac acttcagtga
    21421 tggtaaccag gaggcaagag ccaacaagca gggagaagtg aatgagctac aaccccagcc
    21481 tcagtgtgaa gcagagctga aggacaggtt accttaggac atgctcagtt cagagtgatt
    21541 ctacaaac
//