LOCUS       KO-first_condition_ready_39628_MGI:2442258        21172 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Amz1 targeting project(s): 39628
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_gt1
COMMENT     design_id: 45133
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     exon            5028..5195
                     /db_xref="ENSEMBL:ENSMUSE00000369822"
                     /rank="3"
                     /note="ENSMUSE00000369822"
     misc_feature    4112..8683
                     /note="5 arm"
     misc_feature    8711..8735
                     /note="B1 Gateway"
     misc_feature    8748..8795
                     /note="Frt"
     intron          8796..9670
                     /note="mouse En2 intron"
     misc_feature    9668..9670
                     /note="Splice Acceptor"
     exon            9671..9709
                     /note="mouse En2 exon"
     misc_feature    9710..9726
                     /note="Frame 1 insert"
     misc_feature    9727..9780
                     /note="'self-cleaving' 2A peptide"
     gene            9781..12858
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    12859..12912
                     /note="'self-cleaving' 2A peptide"
     gene            12913..13704
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    13903..14145
                     /note="SV40 polyadenylation site"
     misc_feature    14162..14209
                     /note="Frt"
     misc_feature    14216..14249
                     /note="loxP"
     misc_feature    9685..9800
                     /note="FCHK std_gt1"
     misc_feature    9685..9702
                     /note="FCHK QC prefix region"
     misc_feature    9703..9782
                     /note="FCHK QC critical region"
     misc_feature    9783..9800
                     /note="FCHK QC suffix region"
     misc_feature    14265..14285
                     /note="B2 Gateway"
     misc_feature    8684..14315
                     /note="Synthetic Cassette"
     exon            14696..14824
                     /db_xref="ENSEMBL:ENSMUSE00000412433"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000412433"
     exon            15168..15340
                     /db_xref="ENSEMBL:ENSMUSE00000366789"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00000366789"
     exon            15708..15884
                     /db_xref="ENSEMBL:ENSMUSE00000358034"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 3 ENSMUSE00000358034"
     misc_feature    14316..16080
                     /note="Critical Region"
     misc_feature    16104..16137
                     /note="Downstream LoxP"
     misc_feature    16081..16160
                     /note="synthetic loxP region"
     exon            18532..21172
                     /db_xref="ENSEMBL:ENSMUSE00000950040"
                     /rank="7"
                     /note="ENSMUSE00000950040 5' fragment"
     misc_feature    16161..21172
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg atcactctat ccacgtcatt ggctgggtct
     4141 tggtaggagg accaggttgg acctagaatc tgtcttctcc cctccctctt tccatccctc
     4201 ccttcccctc ttctttccat cctttttaaa atttttaata tgtgtatttg tatctgtgtg
     4261 tactcttgtg catgtgagtg cctatgccct tagagacgag agttgtcttc tctggagtca
     4321 cacgcagttg taagctgccc aacatgggtg ctgggacttg aacttgtgtt ctgtacacga
     4381 gtgagtggtc tgtgctcgga actgttgtgc gtctctccag ccctcttagt attttttttt
     4441 ttttttgata tgggggattt catgcagtct gggctggccc tgaactcatt atttagccaa
     4501 agatgacctt aaactcccgc ccacctccac ctcctgggtc ctgggatgat aggtgtgcac
     4561 caccatgccc aggtttacag tgagggggta gatcctggcc atgtgcatgc tctgtaaacg
     4621 ttctaccaac gcagccgcat ccccagcccc caagcctgga tttctaacag ctttggagac
     4681 catgcaacca cagacccctt gttgccatcc tagatctgta ccctgtccaa gtagcacagg
     4741 ataagcttgt gcccctccct ctggtggctg ttccctgtcg cagatttcct gacttaccac
     4801 tgtcaaccga tgcctctgtc ctagcccagc tgtgtttctc ttggtatagt gccggctagc
     4861 ttctgcctat gccagctggc caagcttaca tgcctttgtc tacctagagt taccacacgg
     4921 ggctgcctgc cctgtaagga aactggcttg tttcacccaa ggggccagca tggatttgcc
     4981 cactgggcag gaggccacat caggctgatg ggctcttgtg ttgctagatc tgagcgaggg
     5041 gctggcggga tgccccctgc tggaccacct tcggagctgc gccgaggctt tcttcctggg
     5101 ccttcgcgtc aaatgcctgc cctcagtggc tgccgcctcc attaactgct gctcaagacc
     5161 cagtcgggat actgatgggc ttcaacttca cacaggtaag cgaggcgggc tgtggggcag
     5221 cctggggctc cgctgtgcag cagaagaccc ttccagaagg aaaagttgag aagaaggagc
     5281 cctgggaaag gggaggaaca tttccaatgt ttgttatcta tgcaggcgtg tgtgtgtgtg
     5341 tgtgtgtgtg tgtatgtgtg tgtgtgcagg gccagaggac aaccttggat atcagtcctc
     5401 agtagctgtc cactttattt tattaagaca ggctctctca ctggtttgga ccttgccaag
     5461 ttggctaagc tgattggcca gtgagcccca gagatgcagc tggctctacc tccccagctc
     5521 tgggattaaa atcacaaact actgcgactg atttatataa ttaagacttt aaaagattgt
     5581 tcactttctt ctacgtgtgt gagtgtttgt ctgtgtgtat gcatgcctgg tgcctgtaga
     5641 gttcagaaga gggtcttgga tcccttggaa ctggagttcc tgactggtgt gagctgccat
     5701 ggaggtgctg ggaacagaac ctgggtcctc cgcaaaggca gccggcactc ttaaatgctg
     5761 agccatctcc tatcacctct cctagcttta aagctaatga tcatgtttac ccacgtgcat
     5821 attaatatgc ttctgttgat gtatgtggat caatgcgtgc ggggccacag gctggagggc
     5881 gttgtaggct ctggaactgg agttacagga ggttgtgaga ctccacgagg gtggcgggac
     5941 tgagccacac cctctgctcc tctccacctt tgtgggcttt ttattaagac aggagcccat
     6001 gtagcccagg ctggctttct ccttgtgatc ctcctgcctc agctcctggc ttgagtgctg
     6061 agattaacag tgcccagaaa cttactagtt ttgatcatta gtccaaggct ttttttggaa
     6121 gacatttcca ggtgatcttg gtcctgggat agttttcctt cagaagggtt ttctcttccc
     6181 cttacacagc cacgtgtgat gaaggcatga ttctattctg ggctgttctg tgacccttgg
     6241 ggagcaatct gaaccccgtg gcagcaggca gagtagggac ctgtctctag ggtctgtgtc
     6301 ctgccttagg agcagcccag cttctctgca ctgtggccgg tcttctcgtg gctctgcttt
     6361 ctcagcaggt atctggttta gatataattc attgaggtca gctgaaggag tacagagctc
     6421 ttagcctaat tacttggagg ctaaggcagg aggatttaaa gttttaggtt tgcctgcact
     6481 acaaagtaag tttgagacca gcttaggcta catatggaga ccctgtttta aaaagtaaaa
     6541 agagggcgtg gctcagtggt agagcccctg cctagaatcc tccagtgagg gctggggcgt
     6601 ggctcagtgg tagagcccct gcctagaatc cctcagtgag gggctggggc gtggctcagt
     6661 ggtagagaac atctgtatag gatattcaga gccctgaaaa cactgaaaaa aatgtctgta
     6721 gggtagtttt gtttacatca aatattttat caaaatcagt ttacttcttt ttttgtttta
     6781 cagttttgag acttttattt tttacttatt catttcctta ttcaattttg agactgggtg
     6841 tcagtaggta gccctggatg tcctagagct cacagaggtc tgcctgctgt ctgccttcag
     6901 agagctggga ttaaaggcct atgccaccag actggccatt ttttatttat ggttcttttt
     6961 tgtttgtttg tttttgtttt ttgtttttag tttttttttt tttttttttg agacagggtt
     7021 tctctgtgta gctctgactg ttctggaact cactctgtag accaggctgg cctcgaactc
     7081 aggaatccgc ctgcctctgc ctcctgagtg ctgggattaa aggcgtgcgc aaccactgcc
     7141 tggctaattt ttatattttt atattatttt ataaaaaaag tgtttggact tttatatatg
     7201 tgcccttcct tccttccatc cttccttcct tccctccctc cctcctttct ctctctctcc
     7261 ctctcttcct tctttctttc tttctttctt tctttctttc tttctatgta tggatgctct
     7321 gtcgcatgta cacctgcaca ccagaagagg gcatcagatc cctttgtaga tggttgtgag
     7381 tcattatgta ggcattgaac tcaggacctc tggaagagca gccagtgctc tttaggacgg
     7441 agccatctca ccagcccttt ctaaaacgta gggacagtgg tacaccccac atttctgtaa
     7501 ccagcaacca catcacctgc actttggact gtgaacacgt cctcctgaca acccctccat
     7561 ccctcactct gcctctgtct tcccaactag gatggtctca agctggtcac atagccctgt
     7621 gctcttaccc tctctgtctc cctgtgtctc agtcctctcc cacacttgac agctctccct
     7681 tacccaccac tccctgtatc atccccgagt tccatcctcc gagtgggatg cagctgggac
     7741 attctcccca gagatgcctg cccttagcgg tactcgcaag tccttggccc ccctctcctt
     7801 cactggcctg cctctgttcc agtgcgctct ttcccctctg tactgtgtca caaccgcgga
     7861 gaggctccac tccatgcttt gttagcagcg cctagcagac ccagacgcag ctgcttccct
     7921 ctctcgtgct gttctccatc ttgatcttca ccaaggcgct cctagtccca ttgccttcct
     7981 tctctgcctc tgtccacagc agacttcctt gggcagctct tttgtctcgt gtcctttcct
     8041 cctctgtggt cactcccctg gctttccagg gccccactgg ccacccaggt ctccctcttc
     8101 tccctccttc ttccctgagt agctcaggtt ccttgttctg accttgactc tctgggcgat
     8161 gcagagccac gatcctcttc acccacagtc acagctgagg gacctctcac tgggcctagg
     8221 ggtctgggtg tgggtatttt ttgggtaact attcaggtgc ctgttctttc ctgggctctt
     8281 tttgccatgt gtgtgtgtgt atgtgcatgt gtgtgtcttc cagtgtgaga gagtatgtgt
     8341 gtgtgtgtgt gtgtgaatgt gtgtgagtat gcatgagagt ctatgactgt gttaatatgt
     8401 gtgtgaatgt gtgtgtgagg ctatgtgaat gtgtgtgtgc gaatgtgtga atttgagagt
     8461 gtatgagtgt gcgtgaatat gtgtgtgtga gagagtgtgt atgtgtgtga gagagtacat
     8521 gagtgtgaga atatgtgtat atgagagagt gtatgtgtga gtgtgtatat gtatgtgtgt
     8581 aagagagtac gtgtgtgtga gagagtactt gtgggggtgg caggggggat gtcataggtc
     8641 tggtcttcct cattcactga tatccctttt ggttttcttg aagaaggcgc ataacgatac
     8701 cacgatatca acaagtttgt acaaaaaagc aggctggcgc cggaaccgaa gttcctattc
     8761 cgaagttcct attctctaga aagtatagga acttcgaacc ctttcccaca ccaccctcca
     8821 cacttgcccc aaacactgcc aactatgtag gaggaagggg ttgggactaa cagaagaacc
     8881 cgttgtgggg aagctgttgg gagggtcact ttatgttctt gcccaaggtc agttgggtgg
     8941 cctgcttctg atgaggtggt cccaaggtct ggggtagaag gtgagaggga caggccacca
     9001 aggtcagccc ccccccccta tcccatagga gccaggtccc tctcctggac aggaagactg
     9061 aaggggagat gccagagact cagtgaagcc tggggtaccc tattggagtc cttcaaggaa
     9121 acaaacttgg cctcaccagg cctcagcctt ggctcctcct gggaactcta ctgcccttgg
     9181 gatccccttg tagttgtggg ttacatagga agggggacgg gattcccctt gactggctag
     9241 cctactcttt tcttcagtct tctccatctc ctctcacctg tctctcgacc ctttccctag
     9301 gatagacttg gaaaaagata aggggagaaa acaaatgcaa acgaggccag aaagattttg
     9361 gctgggcatt ccttccgcta gcttttattg ggatccccta gtttgtgata ggccttttag
     9421 ctacatctgc caatccatct cattttcaca cacacacaca ccactttcct tctggtcagt
     9481 gggcacatgt ccagcctcaa gtttatatca ccacccccaa tgcccaacac ttgtatggcc
     9541 ttgggcgggt catccccccc cccaccccca gtatctgcaa cctcaagcta gcttgggtgc
     9601 gttggttgtg gataagtagc tagactccag caaccagtaa cctctgccct ttctcctcca
     9661 tgacaaccag gtcccaggtc ccgaaaacca aagaagaaga acgcagatcg cagatcgcag
     9721 atctccgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     9781 gggatctgga ctctagagga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9841 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     9901 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
     9961 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
    10021 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
    10081 accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
    10141 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
    10201 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
    10261 tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
    10321 gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct ggaagatcag
    10381 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
    10441 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
    10501 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
    10561 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
    10621 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
    10681 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
    10741 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
    10801 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
    10861 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
    10921 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg
    10981 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
    11041 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta
    11101 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
    11161 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
    11221 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
    11281 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
    11341 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
    11401 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc
    11461 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
    11521 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag
    11581 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca
    11641 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
    11701 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt
    11761 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt
    11821 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta
    11881 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg
    11941 cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg
    12001 accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc
    12061 cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg
    12121 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
    12181 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
    12241 gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac
    12301 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt
    12361 agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg
    12421 cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta
    12481 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
    12541 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
    12601 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc
    12661 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
    12721 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
    12781 ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg
    12841 tgtcagggga tcccccggga gggcagagga agtcttctaa catgcggtga cgtggaggag
    12901 aatcccggcc ctattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
    12961 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
    13021 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
    13081 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
    13141 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
    13201 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
    13261 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
    13321 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
    13381 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
    13441 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
    13501 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
    13561 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
    13621 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
    13681 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
    13741 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
    13801 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
    13861 agttcttcgc ccaccccccg gatctaagct ctagataagt aatgatcata atcagccata
    13921 tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
    13981 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
    14041 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    14101 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccggggg taccgcgtcg
    14161 agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcg tcgagataac
    14221 ttcgtatagc atacattata cgaagttatg tcgagatatc tagacccagc tttcttgtac
    14281 aaagtggttg atatctctat agtcgcagta ggcggaatta caagcccctg ggactggctt
    14341 tcttacctgg gtcatgggga ttagactcgg gctctgtgtc tgtatggcaa acactacact
    14401 cacctcagcc ctcgactgcc ccttcggcct cctcatatgg aaggtgaggg ccttgttata
    14461 gccagatcgg ctctcatggc tcaggagaca gagacccagg acgtgactgg ccctagggat
    14521 ccatggcctc actctcaggc caggtccatg ctccttggtc atctcctctt tagctgatct
    14581 gggttccggg tctcaccaca gatgtggctt gctgcatcca gctttgagag gggctggtcc
    14641 cagaccaagg gatggaatgg gaggctcatc tgcttgccct accttcttcc tgcagatggc
    14701 atcttgtcct tcttgaagaa caacaagcca ggggatgcct tgtgcgtgct gggcctcacg
    14761 ctggctgacc tgtaccccca tgatgcctgg accttcacct tcggcagatt tcttccgggg
    14821 cacggtgagc tgaggtccta gcaactgttg cctaggaggc agaatgatgt ggtcttagca
    14881 caccagtgca ggacatgcat atatgttctc tcctgggggt gggggtgggg gtaggggtgg
    14941 ggtgggggtg ggggtagggg tggggtgggg tgtgtgcaca agcttgaggc tatgttctca
    15001 cgggccctgg gcaaggggct ggtccccaga gtcaactgca tatggcaggg ccaggggacc
    15061 acttcctctt ctggctgtcc tgggctgcag gaagtgctgg ggtggtttca ccctagattc
    15121 ctgagccatg gctgtccttt ggactacttc ttctggcttc tccccagaag tgggtgtgtg
    15181 tagcttcgct cgcttctctg gagagttctt gcaggccggg tccagtgttc ctgactcagc
    15241 tctgctggag gcagcagcag caggtggccc cgagacgctg cctcgtgagc gaggacggac
    15301 cctgtgcttc agcgccctgg gcatggttca gtgctgcaag gtgagcacag gagacaggaa
    15361 ggctcagagg ccccttgcgt agtcagtgct aaaaggggtc cctaacttta aagagtgagc
    15421 gatccaagac taaaagaccc cacggctcaa gaccacaaag agatgaccca aagggttctg
    15481 gggtctggcc ctgggtctgg ctggcttcta agcctcctca tgctgtgtgg cctcttagag
    15541 ccactacgga ggacgtagta agggggatgc ttggatcact gcctagggat ccgttgttac
    15601 ccagggtgtt ggacttcttc cagggttgcc cagtggcttg gggatcccta ggtgggagtt
    15661 ccaggatgct gagactgagg cgaggcttgg taccttgtcc cccttaggtc acctgccatg
    15721 agctctgcca cctcctgggc ctggggagct gtcgctggct ccgctgcctc ctgcaggggg
    15781 cgcttagcct ggatgaggtg ctccgtcggc ccctggacct ctgtcccatc tgcttgagga
    15841 agttgcatca cctcctgggc ttcaggctcc tggagaggta caaggtgagg cggggaagcc
    15901 acacagagga agtgagaggg gacagagtgt gtgtgtgtat agagagagac agagagatag
    15961 agatagagac agagagaaca ccaaatgaca tgcatgcacc atcattccag agggtagggt
    16021 gggctgcggg tctttctggc agatggaaca ctcaggctgg tggcagtagc agtggcatca
    16081 gagatggcgc aacgcaatta atgataactt cgtatagcat acattatacg aagttatggt
    16141 ctgagctcgc catcagttca cctccataag tgctatattt acagagcatt ggtaaggctc
    16201 cttctctaca tgtccatgtc ctctttcctc ccctcggctc cgtggtggac acgggagatc
    16261 ctgtacccag aggtagtgca gctcctagga caaaggggaa gttggtgtcc agaggggcag
    16321 ccagccacag agcctctctt ggctgagctg aagaggaggt gctgccctct gctgtcagca
    16381 tgaagtgttg ctacaagtaa cctccagcct ttatctctgt aggaaggctc gtttgcaaac
    16441 catgacatag gctcagggtt tgaggtcaga tacaaatgct gcttattttt ctcgaaatcc
    16501 accttaggtt gaaatcaagc taaaggtttc agtgcatgga gggcatatcc ctgtgtgtgt
    16561 aaaaatcagc tgtttccagt gcctaggctc aacttactta cttgtgggaa gcctctctcc
    16621 ccacaggcat tttcttcttt gattttgtgg tgttggttca acctttaatc ccagcactcc
    16681 agtggcagag ccaggcatct cagtgagttt gaggccagca tggtctacat agtgagttcc
    16741 aggacagcca gaactacata ggaagaccct gttttgaaac taacaaacaa aaacaaaaca
    16801 aaatcaacaa accagaaaga aaaacggcaa agctttttat ttttatttgt atgagtgttt
    16861 gcctacatgt atgtggacca tgtgtgtgtc tggtgtccat ggaggtcaga agagggtcag
    16921 gtcccttgag actggagtca cagatggttg tcaggtacca tgtggttgct gggaattgaa
    16981 cctgcgtcct ctggaagagc agacagtgct cttaactggt gaaccatctc tcctgctccc
    17041 tctcagtttt ataaggtttg ttattcatcc atctgtttgt gtgtgcaggt gccatggtgt
    17101 gcatatggag ctgagaggat gtaagtcacc aggagttagg ttggcaccct ggggtggcca
    17161 ggtccttctg ggggtagggc atgggaatta gcagggctct agctgctgca ggaggtgggg
    17221 tcagctcatt gtcactagga cagtccctga ggatctgaca gggggcgcag agctaagtga
    17281 gacctagcct caaagctata tcttgtcaca caatatatag cacatctggg catgtgctgt
    17341 gtcacacaag ggcatccatc tggggatgtg ctttgggaag ctcatgtcac aggacagctg
    17401 atggtaggtg gcatttaggg gcagaaaatg tgatttaaaa acatttgatt tagccgggcg
    17461 tggtggcgca cgcctttaat cccagcactc gggaggcaga ggcaggcgga tttctgagtt
    17521 caaggccagc ctggtctaca gagtgagttc caggacagcc agggctatac agagaaaccc
    17581 tgtctcgaaa acccaaaaaa aaaaaaaaaa aaaaaaaaaa acatttgatt taaaaacatt
    17641 ttacttcaga gttggaggga tgacttaaga atagtttggt ttcagtaccc atgtctggta
    17701 gctcacaact gccagttaac tcctggtttg caggatctga cgccctcttc tggccattgt
    17761 gggtactaca tgtacaggat atatatatta cccagtggaa acaaagtttg aactgtaggg
    17821 gctgggaaga tatcttagtg ggtatgagga tgtgaatttg gtttcctggc agctaataaa
    17881 aagttgggtg tggggcaggc ataagcaacc ctcatgctgg ggacagtagg gtctaagaag
    17941 ctctctagcc agtcagtcta accatggtga gctctcggct cagtgagaga ctgtctcaga
    18001 aaacaaggtg tgagccgggc gtggtggcgc acacctttaa tcccagcact cgggaggctg
    18061 aggcagtcgg atttctgagt tcaaggccgg cctggtctac agagtgagtt ccaggacagc
    18121 taggacagtc agggctatac agagaaaccc tgcctcgaaa aaaaaaaaaa caacaaaaaa
    18181 acgaggcatg aaatgaggat gcgtggtatt gatctctggc ctccacatgc acgtgtgtgt
    18241 accctacaca tgtacgtgtg tgtacatgcg cacacacaca aacacataaa agttcagatt
    18301 aactgtaatc gtcatgtact ttaactctga gcagtggctg ggttaacagc catcttgcca
    18361 agtacgtgtg aaaggtgggc ttctgggagt tggaaagtcc agttcggctg tggcctggac
    18421 tccagtcctc agacccacct ccagggacct catggctcca gctccatgcc ggctcctggg
    18481 gtggtcctca gtgactccat cgccaacctt agcctatatt tcttcccgca gagactccat
    18541 gcttggactc gggtgatggt ggagatatgg tctggtcaag aggctgggga gccatctgtg
    18601 tcggaggaca ccttaccctt cagtgctgac tcaggcatgg gctgtgagag tgacacggag
    18661 cctgtcacca gcccgtcaga gcctgtcacc cctgatgggt ggagtcatcc cttccctgac
    18721 ggacctgaac cagtatctga ggatgggctg ggctctctca tggcctcaga agtgtcgctg
    18781 accctcgggg ggccggtgga cgccatggag gagtatggac agtggctgac tgcgtgcatc
    18841 cagaccctgg agagggaggg agctgaagag gagctgctcc aggtggatgc ggctgtggat
    18901 gccctgagcc ggtgggagat gttcacaggg cagctcccgg tgaccaagca gcacgtgccc
    18961 tgtggcaagg ataatgtggg actgcgcagg gtcctgggag acaagttctc ttccctgagg
    19021 cgccggctga gctcacgaag atttgccaag gccagttctt cccactgccg ctggggggca
    19081 gagaattaac ctggcaagat ggaccagcca atgccgagaa gtcagctcct ggcattattt
    19141 tttttttcct ctgaggatac tcaccaacca ggggtgccca gtgactgagg gtcctctggt
    19201 atctgcaggg ttaaaacaaa ggttccccag aagctaaaga aatgctcttt gggtgcttgc
    19261 ccacagggtt cccatgttgt gtggcctgca gaggacgctg agaggcagga ggttgctaca
    19321 tggttaattg taccctgcag gagggggctc tgggccaccc tgcagcatgg gctatgtggc
    19381 tgtgtatgtc tgtttctatc tctgtgtgtc tgtgcacgtg agtgcagttg cccacagagg
    19441 ccagaagagg gcagcagagg ccattacagg aagttgtaag ccaccttaca tgtggcttgt
    19501 ttgcaattga actcaggtcc tctgcaagtg cagcccacat ctttaatgtc tgagccatct
    19561 ctctggcacc aacatttgat gttgacctgt gttgctaaca cggggttctt ctccatggct
    19621 agaatgcctt tctgctcacc agggctggca caggtgccat tgagaagatc ctgaggacat
    19681 ttgtgccctg tgaagctggc agacgtgggg gtgatggggc tggctgtgga agacagaggc
    19741 cacttctcta accctggggc tgctgctgcc accagttagg ccagtgtggt ggttggggct
    19801 gagctctttt gtctttgatg acatttttcc tgtctctggc tatatcaatt cctgacatag
    19861 gatgttaaaa ttatacatcc acagctgtgc tgtgttctac ctttgtttct gtgtcatgta
    19921 ttgttgaagc tcttacacac acacacacac acacacacac acacagattg ttttcctgaa
    19981 gtgactctca ttgttctcaa acgccagcaa cgccagtctc ctggtcctga ggcaggagga
    20041 gggcgggctg tgggcgctct ccattgtgga cagaggcagt aagggacaga ggcatgtgca
    20101 tcgttaggtg agtgactgtg ttcccttttc aatgctgtga aaaacaccca agcaaatcaa
    20161 tttcaaggag ggaagagtgg ttttggctca ggcttccagg gtttcactat ggtcagggtc
    20221 catggctttt aggctgtggt aaggatgagg tagagcatca tgctaggaac ggtgcccgtg
    20281 gagcagaggc cgtgaagcaa agtgggaggg gccagtgaac tatctatcct gtaagggaaa
    20341 attcccactg cttccttccg gttaggctcc cctcctggtt cttaccgcta ccccacctcc
    20401 tcgttttttt atttttttat tttttaatta ttattttttt aatttctgtt tttcttttga
    20461 gctaaagtgt cagtagccca ggttggtctc ttgtgtagcc aaagatgacc ttggagtgtc
    20521 ttgtctgctt agcttcgtgc tgctgagtct cctgtgcccc ctgtgttgct aggacggcca
    20581 gcatgtgttt ccttttctgg ctttattggc agggcagcag agcctggacc atgcctcttc
    20641 aggtgccctg atgtcccctc ctctccccag agcccagact cctgtcagag gagaaggcag
    20701 gccagcattt tgtgacacag actgttgggg acagaagatt tgagtttcac ctcatgacca
    20761 ggaaggagat gctcctggga cagagacctt tgtggatgga cagaccctcc aatgttaaaa
    20821 gcacagacat gcagtggttt agctggggag ccaggggtaa agctaaagtt caaacttcga
    20881 tcccgctgag gttgaagggg atgctagaac attctaggga tgtgtccaca tacagaaggt
    20941 ctgtgagcag tgctaaggtg agccactctg gatccaacag gcagagacag ccaggttcta
    21001 gagggacaga cagctctacc caagcctgcc aggaaggtca agaaaggttt gaaaagcaac
    21061 gtgtgtgtgt gtctggtgtg tgtctgtgtg tgtacctgtg tgtgcccaca tgtacaggtt
    21121 tgaggccccc cttagatgtt tgattttttt ttgtttcgat gagatcaggt ct
//