LOCUS       KO-first_condition_ready_39629_MGI:2442258        22825 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Amz1 targeting project(s): 39629
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 45133
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            5191..5358
                     /db_xref="ENSEMBL:ENSMUSE00000369822"
                     /rank="3"
                     /note="ENSMUSE00000369822"
     misc_feature    4275..8846
                     /note="5 arm"
     misc_feature    8874..8898
                     /note="B1 Gateway"
     misc_feature    8911..8958
                     /note="Frt"
     intron          8959..9833
                     /note="mouse En2 intron"
     misc_feature    9831..9833
                     /note="Splice Acceptor"
     exon            9834..10006
                     /note="mouse En2 exon"
     misc_feature    10018..10611
                     /note="ECMV IRES"
     gene            10612..13672
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13711..13954
                     /note="SV40 polyadenylation site"
     misc_feature    13958..13991
                     /note="loxP"
     promoter        14018..14537
                     /note="human beta actin promoter"
     gene            14563..15357
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15557..15798
                     /note="SV40 polyadenylation site"
     misc_feature    15815..15862
                     /note="Frt"
     misc_feature    15869..15902
                     /note="loxP"
     misc_feature    9848..9925
                     /note="FCHK std_gpr"
     misc_feature    15918..15938
                     /note="B2 Gateway"
     misc_feature    8847..15968
                     /note="Synthetic Cassette"
     exon            16349..16477
                     /db_xref="ENSEMBL:ENSMUSE00000412433"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 1 ENSMUSE00000412433"
     exon            16821..16993
                     /db_xref="ENSEMBL:ENSMUSE00000366789"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 2 ENSMUSE00000366789"
     exon            17361..17537
                     /db_xref="ENSEMBL:ENSMUSE00000358034"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 3 ENSMUSE00000358034"
     misc_feature    15969..17733
                     /note="Critical Region"
     misc_feature    17757..17790
                     /note="Downstream LoxP"
     misc_feature    17734..17813
                     /note="synthetic loxP region"
     exon            20185..22825
                     /db_xref="ENSEMBL:ENSMUSE00000950040"
                     /rank="7"
                     /note="ENSMUSE00000950040 5' fragment"
     misc_feature    17814..22825
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatcactc tatccacgtc attggctggg tcttggtagg aggaccaggt
     4321 tggacctaga atctgtcttc tcccctccct ctttccatcc ctcccttccc ctcttctttc
     4381 catccttttt aaaattttta atatgtgtat ttgtatctgt gtgtactctt gtgcatgtga
     4441 gtgcctatgc ccttagagac gagagttgtc ttctctggag tcacacgcag ttgtaagctg
     4501 cccaacatgg gtgctgggac ttgaacttgt gttctgtaca cgagtgagtg gtctgtgctc
     4561 ggaactgttg tgcgtctctc cagccctctt agtatttttt tttttttttg atatggggga
     4621 tttcatgcag tctgggctgg ccctgaactc attatttagc caaagatgac cttaaactcc
     4681 cgcccacctc cacctcctgg gtcctgggat gataggtgtg caccaccatg cccaggttta
     4741 cagtgagggg gtagatcctg gccatgtgca tgctctgtaa acgttctacc aacgcagccg
     4801 catccccagc ccccaagcct ggatttctaa cagctttgga gaccatgcaa ccacagaccc
     4861 cttgttgcca tcctagatct gtaccctgtc caagtagcac aggataagct tgtgcccctc
     4921 cctctggtgg ctgttccctg tcgcagattt cctgacttac cactgtcaac cgatgcctct
     4981 gtcctagccc agctgtgttt ctcttggtat agtgccggct agcttctgcc tatgccagct
     5041 ggccaagctt acatgccttt gtctacctag agttaccaca cggggctgcc tgccctgtaa
     5101 ggaaactggc ttgtttcacc caaggggcca gcatggattt gcccactggg caggaggcca
     5161 catcaggctg atgggctctt gtgttgctag atctgagcga ggggctggcg ggatgccccc
     5221 tgctggacca ccttcggagc tgcgccgagg ctttcttcct gggccttcgc gtcaaatgcc
     5281 tgccctcagt ggctgccgcc tccattaact gctgctcaag acccagtcgg gatactgatg
     5341 ggcttcaact tcacacaggt aagcgaggcg ggctgtgggg cagcctgggg ctccgctgtg
     5401 cagcagaaga cccttccaga aggaaaagtt gagaagaagg agccctggga aaggggagga
     5461 acatttccaa tgtttgttat ctatgcaggc gtgtgtgtgt gtgtgtgtgt gtgtgtatgt
     5521 gtgtgtgtgc agggccagag gacaaccttg gatatcagtc ctcagtagct gtccacttta
     5581 ttttattaag acaggctctc tcactggttt ggaccttgcc aagttggcta agctgattgg
     5641 ccagtgagcc ccagagatgc agctggctct acctccccag ctctgggatt aaaatcacaa
     5701 actactgcga ctgatttata taattaagac tttaaaagat tgttcacttt cttctacgtg
     5761 tgtgagtgtt tgtctgtgtg tatgcatgcc tggtgcctgt agagttcaga agagggtctt
     5821 ggatcccttg gaactggagt tcctgactgg tgtgagctgc catggaggtg ctgggaacag
     5881 aacctgggtc ctccgcaaag gcagccggca ctcttaaatg ctgagccatc tcctatcacc
     5941 tctcctagct ttaaagctaa tgatcatgtt tacccacgtg catattaata tgcttctgtt
     6001 gatgtatgtg gatcaatgcg tgcggggcca caggctggag ggcgttgtag gctctggaac
     6061 tggagttaca ggaggttgtg agactccacg agggtggcgg gactgagcca caccctctgc
     6121 tcctctccac ctttgtgggc tttttattaa gacaggagcc catgtagccc aggctggctt
     6181 tctccttgtg atcctcctgc ctcagctcct ggcttgagtg ctgagattaa cagtgcccag
     6241 aaacttacta gttttgatca ttagtccaag gctttttttg gaagacattt ccaggtgatc
     6301 ttggtcctgg gatagttttc cttcagaagg gttttctctt ccccttacac agccacgtgt
     6361 gatgaaggca tgattctatt ctgggctgtt ctgtgaccct tggggagcaa tctgaacccc
     6421 gtggcagcag gcagagtagg gacctgtctc tagggtctgt gtcctgcctt aggagcagcc
     6481 cagcttctct gcactgtggc cggtcttctc gtggctctgc tttctcagca ggtatctggt
     6541 ttagatataa ttcattgagg tcagctgaag gagtacagag ctcttagcct aattacttgg
     6601 aggctaaggc aggaggattt aaagttttag gtttgcctgc actacaaagt aagtttgaga
     6661 ccagcttagg ctacatatgg agaccctgtt ttaaaaagta aaaagagggc gtggctcagt
     6721 ggtagagccc ctgcctagaa tcctccagtg agggctgggg cgtggctcag tggtagagcc
     6781 cctgcctaga atccctcagt gaggggctgg ggcgtggctc agtggtagag aacatctgta
     6841 taggatattc agagccctga aaacactgaa aaaaatgtct gtagggtagt tttgtttaca
     6901 tcaaatattt tatcaaaatc agtttacttc tttttttgtt ttacagtttt gagactttta
     6961 ttttttactt attcatttcc ttattcaatt ttgagactgg gtgtcagtag gtagccctgg
     7021 atgtcctaga gctcacagag gtctgcctgc tgtctgcctt cagagagctg ggattaaagg
     7081 cctatgccac cagactggcc attttttatt tatggttctt ttttgtttgt ttgtttttgt
     7141 tttttgtttt tagttttttt tttttttttt ttgagacagg gtttctctgt gtagctctga
     7201 ctgttctgga actcactctg tagaccaggc tggcctcgaa ctcaggaatc cgcctgcctc
     7261 tgcctcctga gtgctgggat taaaggcgtg cgcaaccact gcctggctaa tttttatatt
     7321 tttatattat tttataaaaa aagtgtttgg acttttatat atgtgccctt ccttccttcc
     7381 atccttcctt ccttccctcc ctccctcctt tctctctctc tccctctctt ccttctttct
     7441 ttctttcttt ctttctttct ttctttctat gtatggatgc tctgtcgcat gtacacctgc
     7501 acaccagaag agggcatcag atccctttgt agatggttgt gagtcattat gtaggcattg
     7561 aactcaggac ctctggaaga gcagccagtg ctctttagga cggagccatc tcaccagccc
     7621 tttctaaaac gtagggacag tggtacaccc cacatttctg taaccagcaa ccacatcacc
     7681 tgcactttgg actgtgaaca cgtcctcctg acaacccctc catccctcac tctgcctctg
     7741 tcttcccaac taggatggtc tcaagctggt cacatagccc tgtgctctta ccctctctgt
     7801 ctccctgtgt ctcagtcctc tcccacactt gacagctctc ccttacccac cactccctgt
     7861 atcatccccg agttccatcc tccgagtggg atgcagctgg gacattctcc ccagagatgc
     7921 ctgcccttag cggtactcgc aagtccttgg cccccctctc cttcactggc ctgcctctgt
     7981 tccagtgcgc tctttcccct ctgtactgtg tcacaaccgc ggagaggctc cactccatgc
     8041 tttgttagca gcgcctagca gacccagacg cagctgcttc cctctctcgt gctgttctcc
     8101 atcttgatct tcaccaaggc gctcctagtc ccattgcctt ccttctctgc ctctgtccac
     8161 agcagacttc cttgggcagc tcttttgtct cgtgtccttt cctcctctgt ggtcactccc
     8221 ctggctttcc agggccccac tggccaccca ggtctccctc ttctccctcc ttcttccctg
     8281 agtagctcag gttccttgtt ctgaccttga ctctctgggc gatgcagagc cacgatcctc
     8341 ttcacccaca gtcacagctg agggacctct cactgggcct aggggtctgg gtgtgggtat
     8401 tttttgggta actattcagg tgcctgttct ttcctgggct ctttttgcca tgtgtgtgtg
     8461 tgtatgtgca tgtgtgtgtc ttccagtgtg agagagtatg tgtgtgtgtg tgtgtgtgaa
     8521 tgtgtgtgag tatgcatgag agtctatgac tgtgttaata tgtgtgtgaa tgtgtgtgtg
     8581 aggctatgtg aatgtgtgtg tgcgaatgtg tgaatttgag agtgtatgag tgtgcgtgaa
     8641 tatgtgtgtg tgagagagtg tgtatgtgtg tgagagagta catgagtgtg agaatatgtg
     8701 tatatgagag agtgtatgtg tgagtgtgta tatgtatgtg tgtaagagag tacgtgtgtg
     8761 tgagagagta cttgtggggg tggcaggggg gatgtcatag gtctggtctt cctcattcac
     8821 tgatatccct tttggttttc ttgaagaagg cgcataacga taccacgata tcaacaagtt
     8881 tgtacaaaaa agcaggctgg cgccggaacc gaagttccta ttccgaagtt cctattctct
     8941 agaaagtata ggaacttcga accctttccc acaccaccct ccacacttgc cccaaacact
     9001 gccaactatg taggaggaag gggttgggac taacagaaga acccgttgtg gggaagctgt
     9061 tgggagggtc actttatgtt cttgcccaag gtcagttggg tggcctgctt ctgatgaggt
     9121 ggtcccaagg tctggggtag aaggtgagag ggacaggcca ccaaggtcag cccccccccc
     9181 ctatcccata ggagccaggt ccctctcctg gacaggaaga ctgaagggga gatgccagag
     9241 actcagtgaa gcctggggta ccctattgga gtccttcaag gaaacaaact tggcctcacc
     9301 aggcctcagc cttggctcct cctgggaact ctactgccct tgggatcccc ttgtagttgt
     9361 gggttacata ggaaggggga cgggattccc cttgactggc tagcctactc ttttcttcag
     9421 tcttctccat ctcctctcac ctgtctctcg accctttccc taggatagac ttggaaaaag
     9481 ataaggggag aaaacaaatg caaacgaggc cagaaagatt ttggctgggc attccttccg
     9541 ctagctttta ttgggatccc ctagtttgtg ataggccttt tagctacatc tgccaatcca
     9601 tctcattttc acacacacac acaccacttt ccttctggtc agtgggcaca tgtccagcct
     9661 caagtttata tcaccacccc caatgcccaa cacttgtatg gccttgggcg ggtcatcccc
     9721 ccccccaccc ccagtatctg caacctcaag ctagcttggg tgcgttggtt gtggataagt
     9781 agctagactc cagcaaccag taacctctgc cctttctcct ccatgacaac caggtcccag
     9841 gtcccgaaaa ccaaagaaga agaaccctaa caaagaggac aagcggcctc gcacagcctt
     9901 cactgctgag cagctccaga ggctcaaggc tgagtttcag accaacaggt acctgacaga
     9961 gcagcggcgc cagagtctgg cacaggagct cggtacccgg aagatctgga ctctagagaa
    10021 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
    10081 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
    10141 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
    10201 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
    10261 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
    10321 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
    10381 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
    10441 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
    10501 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc
    10561 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac catggaagat
    10621 cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    10681 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    10741 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
    10801 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
    10861 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt
    10921 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
    10981 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
    11041 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg
    11101 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
    11161 gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc
    11221 attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt
    11281 gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc
    11341 ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc
    11401 gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
    11461 cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat
    11521 ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc
    11581 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
    11641 ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
    11701 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg
    11761 cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg
    11821 tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc
    11881 gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat
    11941 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat
    12001 cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa
    12061 ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat
    12121 gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct
    12181 ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc
    12241 ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc
    12301 tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct
    12361 tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc
    12421 tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc
    12481 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
    12541 gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa
    12601 gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag
    12661 ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca
    12721 tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt
    12781 gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt
    12841 tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag
    12901 atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca
    12961 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc
    13021 gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca
    13081 gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa
    13141 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc
    13201 gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag
    13261 ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc
    13321 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc
    13381 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
    13441 ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg
    13501 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
    13561 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc
    13621 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg
    13681 ggcagggggg atctaagctc tagataagta atgatcataa tcagccatat cacatctgta
    13741 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    13801 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    13861 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    13921 aaactcatca atgtatctta tcatgtctgg atccggaata acttcgtata gcatacatta
    13981 tacgaagtta tgtttaaacg gcgcgccccg gaattcgcct tctgcaggag cgtacagaac
    14041 ccagggccct ggcacccgtg cagaccctgg cccaccccac ctgggcgctc agtgcccaag
    14101 agatgtccac acctaggatg tcccgcggtg ggtggggggc ccgagagacg ggcaggccgg
    14161 gggcaggcct ggccatgcgg ggccgaaccg ggcactgccc agcgtgggcg cgggggccac
    14221 ggcgcgcgcc cccagccccc gggcccagca ccccaaggcg gccaacgcca aaactctccc
    14281 tcctcctctt cctcaatctc gctctcgctc tttttttttt tcgcaaaagg aggggagagg
    14341 gggtaaaaaa atgctgcact gtgcggcgaa gccggtgagt gagcggcgcg gggccaatca
    14401 gcgtgcgccg ttccgaaagt tgccttttat ggctcgagcg gccgcggcgg cgccctataa
    14461 aacccagcgg cgcgacgcgc caccaccgcc gagaccgcgt ccgccccgcg agcacagagc
    14521 ctcgcctttg ccgatcctct agagtcgaga tccgccgcca ccatgattga acaagatgga
    14581 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
    14641 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
    14701 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
    14761 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
    14821 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
    14881 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
    14941 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
    15001 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
    15061 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
    15121 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
    15181 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
    15241 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
    15301 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
    15361 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
    15421 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
    15481 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc ccggatctaa
    15541 gctctagata agtaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt
    15601 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
    15661 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    15721 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    15781 cttatcatgt ctggatccgg gggtaccgcg tcgagaagtt cctattccga agttcctatt
    15841 ctctagaaag tataggaact tcgtcgagat aacttcgtat agcatacatt atacgaagtt
    15901 atgtcgagat atctagaccc agctttcttg tacaaagtgg ttgatatctc tatagtcgca
    15961 gtaggcggaa ttacaagccc ctgggactgg ctttcttacc tgggtcatgg ggattagact
    16021 cgggctctgt gtctgtatgg caaacactac actcacctca gccctcgact gccccttcgg
    16081 cctcctcata tggaaggtga gggccttgtt atagccagat cggctctcat ggctcaggag
    16141 acagagaccc aggacgtgac tggccctagg gatccatggc ctcactctca ggccaggtcc
    16201 atgctccttg gtcatctcct ctttagctga tctgggttcc gggtctcacc acagatgtgg
    16261 cttgctgcat ccagctttga gaggggctgg tcccagacca agggatggaa tgggaggctc
    16321 atctgcttgc cctaccttct tcctgcagat ggcatcttgt ccttcttgaa gaacaacaag
    16381 ccaggggatg ccttgtgcgt gctgggcctc acgctggctg acctgtaccc ccatgatgcc
    16441 tggaccttca ccttcggcag atttcttccg gggcacggtg agctgaggtc ctagcaactg
    16501 ttgcctagga ggcagaatga tgtggtctta gcacaccagt gcaggacatg catatatgtt
    16561 ctctcctggg ggtgggggtg ggggtagggg tggggtgggg gtgggggtag gggtggggtg
    16621 gggtgtgtgc acaagcttga ggctatgttc tcacgggccc tgggcaaggg gctggtcccc
    16681 agagtcaact gcatatggca gggccagggg accacttcct cttctggctg tcctgggctg
    16741 caggaagtgc tggggtggtt tcaccctaga ttcctgagcc atggctgtcc tttggactac
    16801 ttcttctggc ttctccccag aagtgggtgt gtgtagcttc gctcgcttct ctggagagtt
    16861 cttgcaggcc gggtccagtg ttcctgactc agctctgctg gaggcagcag cagcaggtgg
    16921 ccccgagacg ctgcctcgtg agcgaggacg gaccctgtgc ttcagcgccc tgggcatggt
    16981 tcagtgctgc aaggtgagca caggagacag gaaggctcag aggccccttg cgtagtcagt
    17041 gctaaaaggg gtccctaact ttaaagagtg agcgatccaa gactaaaaga ccccacggct
    17101 caagaccaca aagagatgac ccaaagggtt ctggggtctg gccctgggtc tggctggctt
    17161 ctaagcctcc tcatgctgtg tggcctctta gagccactac ggaggacgta gtaaggggga
    17221 tgcttggatc actgcctagg gatccgttgt tacccagggt gttggacttc ttccagggtt
    17281 gcccagtggc ttggggatcc ctaggtggga gttccaggat gctgagactg aggcgaggct
    17341 tggtaccttg tcccccttag gtcacctgcc atgagctctg ccacctcctg ggcctgggga
    17401 gctgtcgctg gctccgctgc ctcctgcagg gggcgcttag cctggatgag gtgctccgtc
    17461 ggcccctgga cctctgtccc atctgcttga ggaagttgca tcacctcctg ggcttcaggc
    17521 tcctggagag gtacaaggtg aggcggggaa gccacacaga ggaagtgaga ggggacagag
    17581 tgtgtgtgtg tatagagaga gacagagaga tagagataga gacagagaga acaccaaatg
    17641 acatgcatgc accatcattc cagagggtag ggtgggctgc gggtctttct ggcagatgga
    17701 acactcaggc tggtggcagt agcagtggca tcagagatgg cgcaacgcaa ttaatgataa
    17761 cttcgtatag catacattat acgaagttat ggtctgagct cgccatcagt tcacctccat
    17821 aagtgctata tttacagagc attggtaagg ctccttctct acatgtccat gtcctctttc
    17881 ctcccctcgg ctccgtggtg gacacgggag atcctgtacc cagaggtagt gcagctccta
    17941 ggacaaaggg gaagttggtg tccagagggg cagccagcca cagagcctct cttggctgag
    18001 ctgaagagga ggtgctgccc tctgctgtca gcatgaagtg ttgctacaag taacctccag
    18061 cctttatctc tgtaggaagg ctcgtttgca aaccatgaca taggctcagg gtttgaggtc
    18121 agatacaaat gctgcttatt tttctcgaaa tccaccttag gttgaaatca agctaaaggt
    18181 ttcagtgcat ggagggcata tccctgtgtg tgtaaaaatc agctgtttcc agtgcctagg
    18241 ctcaacttac ttacttgtgg gaagcctctc tccccacagg cattttcttc tttgattttg
    18301 tggtgttggt tcaaccttta atcccagcac tccagtggca gagccaggca tctcagtgag
    18361 tttgaggcca gcatggtcta catagtgagt tccaggacag ccagaactac ataggaagac
    18421 cctgttttga aactaacaaa caaaaacaaa acaaaatcaa caaaccagaa agaaaaacgg
    18481 caaagctttt tatttttatt tgtatgagtg tttgcctaca tgtatgtgga ccatgtgtgt
    18541 gtctggtgtc catggaggtc agaagagggt caggtccctt gagactggag tcacagatgg
    18601 ttgtcaggta ccatgtggtt gctgggaatt gaacctgcgt cctctggaag agcagacagt
    18661 gctcttaact ggtgaaccat ctctcctgct ccctctcagt tttataaggt ttgttattca
    18721 tccatctgtt tgtgtgtgca ggtgccatgg tgtgcatatg gagctgagag gatgtaagtc
    18781 accaggagtt aggttggcac cctggggtgg ccaggtcctt ctgggggtag ggcatgggaa
    18841 ttagcagggc tctagctgct gcaggaggtg gggtcagctc attgtcacta ggacagtccc
    18901 tgaggatctg acagggggcg cagagctaag tgagacctag cctcaaagct atatcttgtc
    18961 acacaatata tagcacatct gggcatgtgc tgtgtcacac aagggcatcc atctggggat
    19021 gtgctttggg aagctcatgt cacaggacag ctgatggtag gtggcattta ggggcagaaa
    19081 atgtgattta aaaacatttg atttagccgg gcgtggtggc gcacgccttt aatcccagca
    19141 ctcgggaggc agaggcaggc ggatttctga gttcaaggcc agcctggtct acagagtgag
    19201 ttccaggaca gccagggcta tacagagaaa ccctgtctcg aaaacccaaa aaaaaaaaaa
    19261 aaaaaaaaaa aaaacatttg atttaaaaac attttacttc agagttggag ggatgactta
    19321 agaatagttt ggtttcagta cccatgtctg gtagctcaca actgccagtt aactcctggt
    19381 ttgcaggatc tgacgccctc ttctggccat tgtgggtact acatgtacag gatatatata
    19441 ttacccagtg gaaacaaagt ttgaactgta ggggctggga agatatctta gtgggtatga
    19501 ggatgtgaat ttggtttcct ggcagctaat aaaaagttgg gtgtggggca ggcataagca
    19561 accctcatgc tggggacagt agggtctaag aagctctcta gccagtcagt ctaaccatgg
    19621 tgagctctcg gctcagtgag agactgtctc agaaaacaag gtgtgagccg ggcgtggtgg
    19681 cgcacacctt taatcccagc actcgggagg ctgaggcagt cggatttctg agttcaaggc
    19741 cggcctggtc tacagagtga gttccaggac agctaggaca gtcagggcta tacagagaaa
    19801 ccctgcctcg aaaaaaaaaa aaacaacaaa aaaacgaggc atgaaatgag gatgcgtggt
    19861 attgatctct ggcctccaca tgcacgtgtg tgtaccctac acatgtacgt gtgtgtacat
    19921 gcgcacacac acaaacacat aaaagttcag attaactgta atcgtcatgt actttaactc
    19981 tgagcagtgg ctgggttaac agccatcttg ccaagtacgt gtgaaaggtg ggcttctggg
    20041 agttggaaag tccagttcgg ctgtggcctg gactccagtc ctcagaccca cctccaggga
    20101 cctcatggct ccagctccat gccggctcct ggggtggtcc tcagtgactc catcgccaac
    20161 cttagcctat atttcttccc gcagagactc catgcttgga ctcgggtgat ggtggagata
    20221 tggtctggtc aagaggctgg ggagccatct gtgtcggagg acaccttacc cttcagtgct
    20281 gactcaggca tgggctgtga gagtgacacg gagcctgtca ccagcccgtc agagcctgtc
    20341 acccctgatg ggtggagtca tcccttccct gacggacctg aaccagtatc tgaggatggg
    20401 ctgggctctc tcatggcctc agaagtgtcg ctgaccctcg gggggccggt ggacgccatg
    20461 gaggagtatg gacagtggct gactgcgtgc atccagaccc tggagaggga gggagctgaa
    20521 gaggagctgc tccaggtgga tgcggctgtg gatgccctga gccggtggga gatgttcaca
    20581 gggcagctcc cggtgaccaa gcagcacgtg ccctgtggca aggataatgt gggactgcgc
    20641 agggtcctgg gagacaagtt ctcttccctg aggcgccggc tgagctcacg aagatttgcc
    20701 aaggccagtt cttcccactg ccgctggggg gcagagaatt aacctggcaa gatggaccag
    20761 ccaatgccga gaagtcagct cctggcatta tttttttttt cctctgagga tactcaccaa
    20821 ccaggggtgc ccagtgactg agggtcctct ggtatctgca gggttaaaac aaaggttccc
    20881 cagaagctaa agaaatgctc tttgggtgct tgcccacagg gttcccatgt tgtgtggcct
    20941 gcagaggacg ctgagaggca ggaggttgct acatggttaa ttgtaccctg caggaggggg
    21001 ctctgggcca ccctgcagca tgggctatgt ggctgtgtat gtctgtttct atctctgtgt
    21061 gtctgtgcac gtgagtgcag ttgcccacag aggccagaag agggcagcag aggccattac
    21121 aggaagttgt aagccacctt acatgtggct tgtttgcaat tgaactcagg tcctctgcaa
    21181 gtgcagccca catctttaat gtctgagcca tctctctggc accaacattt gatgttgacc
    21241 tgtgttgcta acacggggtt cttctccatg gctagaatgc ctttctgctc accagggctg
    21301 gcacaggtgc cattgagaag atcctgagga catttgtgcc ctgtgaagct ggcagacgtg
    21361 ggggtgatgg ggctggctgt ggaagacaga ggccacttct ctaaccctgg ggctgctgct
    21421 gccaccagtt aggccagtgt ggtggttggg gctgagctct tttgtctttg atgacatttt
    21481 tcctgtctct ggctatatca attcctgaca taggatgtta aaattataca tccacagctg
    21541 tgctgtgttc tacctttgtt tctgtgtcat gtattgttga agctcttaca cacacacaca
    21601 cacacacaca cacacacaga ttgttttcct gaagtgactc tcattgttct caaacgccag
    21661 caacgccagt ctcctggtcc tgaggcagga ggagggcggg ctgtgggcgc tctccattgt
    21721 ggacagaggc agtaagggac agaggcatgt gcatcgttag gtgagtgact gtgttccctt
    21781 ttcaatgctg tgaaaaacac ccaagcaaat caatttcaag gagggaagag tggttttggc
    21841 tcaggcttcc agggtttcac tatggtcagg gtccatggct tttaggctgt ggtaaggatg
    21901 aggtagagca tcatgctagg aacggtgccc gtggagcaga ggccgtgaag caaagtggga
    21961 ggggccagtg aactatctat cctgtaaggg aaaattccca ctgcttcctt ccggttaggc
    22021 tcccctcctg gttcttaccg ctaccccacc tcctcgtttt tttatttttt tattttttaa
    22081 ttattatttt tttaatttct gtttttcttt tgagctaaag tgtcagtagc ccaggttggt
    22141 ctcttgtgta gccaaagatg accttggagt gtcttgtctg cttagcttcg tgctgctgag
    22201 tctcctgtgc cccctgtgtt gctaggacgg ccagcatgtg tttccttttc tggctttatt
    22261 ggcagggcag cagagcctgg accatgcctc ttcaggtgcc ctgatgtccc ctcctctccc
    22321 cagagcccag actcctgtca gaggagaagg caggccagca ttttgtgaca cagactgttg
    22381 gggacagaag atttgagttt cacctcatga ccaggaagga gatgctcctg ggacagagac
    22441 ctttgtggat ggacagaccc tccaatgtta aaagcacaga catgcagtgg tttagctggg
    22501 gagccagggg taaagctaaa gttcaaactt cgatcccgct gaggttgaag gggatgctag
    22561 aacattctag ggatgtgtcc acatacagaa ggtctgtgag cagtgctaag gtgagccact
    22621 ctggatccaa caggcagaga cagccaggtt ctagagggac agacagctct acccaagcct
    22681 gccaggaagg tcaagaaagg tttgaaaagc aacgtgtgtg tgtgtctggt gtgtgtctgt
    22741 gtgtgtacct gtgtgtgccc acatgtacag gtttgaggcc ccccttagat gtttgatttt
    22801 tttttgtttc gatgagatca ggtct
//