LOCUS       KO-first_condition_ready_38953_MGI:2442184        21268 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Flcn targeting project(s): 38953
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 43661
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     exon            7543..7703
                     /db_xref="ENSEMBL:ENSMUSE00001240392"
                     /rank="6"
                     /note="ENSMUSE00001240392"
     exon            6396..6617
                     /db_xref="ENSEMBL:ENSMUSE00001252309"
                     /rank="5"
                     /note="ENSMUSE00001252309"
     exon            4851..4997
                     /db_xref="ENSEMBL:ENSMUSE00001225530"
                     /rank="4"
                     /note="ENSMUSE00001225530"
     exon            4215..4225
                     /db_xref="ENSEMBL:ENSMUSE00001221283"
                     /rank="3"
                     /note="ENSMUSE00001221283 3' fragment"
     misc_feature    4215..8834
                     /note="5 arm"
     misc_feature    8862..8886
                     /note="B1 Gateway"
     misc_feature    8899..8946
                     /note="Frt"
     intron          8947..9821
                     /note="mouse En2 intron"
     misc_feature    9819..9821
                     /note="Splice Acceptor"
     exon            9822..9994
                     /note="mouse En2 exon"
     misc_feature    10006..10599
                     /note="ECMV IRES"
     gene            10600..13660
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13699..13942
                     /note="SV40 polyadenylation site"
     misc_feature    13946..13979
                     /note="loxP"
     promoter        14006..14525
                     /note="human beta actin promoter"
     gene            14551..15345
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15545..15786
                     /note="SV40 polyadenylation site"
     misc_feature    15803..15850
                     /note="Frt"
     misc_feature    15857..15890
                     /note="loxP"
     misc_feature    9836..9913
                     /note="FCHK std_gpr"
     misc_feature    15906..15926
                     /note="B2 Gateway"
     misc_feature    8835..15956
                     /note="Synthetic Cassette"
     exon            16353..16444
                     /db_xref="ENSEMBL:ENSMUSE00001227063"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 1 ENSMUSE00001227063"
     misc_feature    15957..16649
                     /note="Critical Region"
     misc_feature    16673..16706
                     /note="Downstream LoxP"
     misc_feature    16650..16729
                     /note="synthetic loxP region"
     exon            20579..20702
                     /db_xref="ENSEMBL:ENSMUSE00000247618"
                     /rank="10"
                     /note="ENSMUSE00000247618"
     exon            19980..20093
                     /db_xref="ENSEMBL:ENSMUSE00001298491"
                     /rank="9"
                     /note="ENSMUSE00001298491"
     exon            17598..17788
                     /db_xref="ENSEMBL:ENSMUSE00001306229"
                     /rank="8"
                     /note="ENSMUSE00001306229"
     misc_feature    16730..21268
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggaacatgt gtgaggcaag tgtcctttgg gaccgtgagc tggctttagt
     4261 gtgagaggtg tgttatagtg ggggcatggg gctcccccaa ggccctgagg gagtttttct
     4321 catcacccca gcacgagccc ttctaacctg gtcagtgcag aggctggtct agtctacaga
     4381 gactgtcagg gatacacaga tatacaaccc ctgtcttgaa aaaagaaaag aaacacacat
     4441 acatacgctt aaatgaagtt gtaccaacta aagagaatgc tttctccaac agtcattgca
     4501 aagagggata acacaagctt ctttcttcct tcctttcttt ctttctctct ctctctctct
     4561 ctctctctct ttctttcttt ctttctttct ttctttcttg ttggttttgt ttttttcaag
     4621 accagggttt tctgtgtagc ctacgctgtc ctgggccttt actctgtaga ccaggctggc
     4681 ctcaaactcc tagagattcc tctgcctctg ccttcctagt gctgagatta aaggtgtgcg
     4741 ccatcaccac tgcccagcag tgatttttaa aaaacatttg aaagggattg tgttttctgt
     4801 agatgtgctg cctgcttctg cctaagagat tttttatcct ttgtgcacag ggctgccggt
     4861 cacttgccgt agggcaccca ggctatatca gtcatgataa agagacctct attaagtacg
     4921 tcagtcacca gcaccccaac cacccgcagc tcttcagcat cgtccgccag gcctgtgtcc
     4981 ggagcctgag ctgtgaggtg aggccgatgg cagactgtgg ggaacacgtg gggatgtggg
     5041 ttcgttactg tttatagacg gccaggggct cgcatgtcag tgctatgcga gcatggctgc
     5101 agctgttaga acttgaagag taagtccctg tggactccag ctgtctaaat aggtgttagc
     5161 atttcacttt gaacaacaga ctaaggtttt taaagtccat tcttgacgaa catgtctctg
     5221 agataccaca tttccgaggc tgtgcagctt cctgcgtgta gtatctgtca cctttacatt
     5281 gtatcagcgt ccacatccgc agaaggcttc gggctgcaga gttggttcag tggttaacag
     5341 cactggctgc tcttgagagg acccgagttc aatcccagca accacatgtt ggctcaaaac
     5401 tgtctataac tccagcccag gggatctgac accttcttct ggccccccat ggccactgca
     5461 cacatgtgtg ccacagacat atatactagc aaacactcat aagcagagac taaaaataag
     5521 ggtttaaaaa caaacaaaca aacaaacaaa gaatgatttg ctcctgaact cctgctgtgg
     5581 ggccctagtg tcagctctta ggtttgacct gtcacacggg catatatggc cctagtgtca
     5641 gctcttaggt ttgacctgtc acacaggcat atatggccct agtgtcagct cttaggtttg
     5701 acctgtcaca caggcatata tggccctagt gtcagctctt aggtttgacc tgtcacacag
     5761 gcatatatgg ccctagtgtc agctcttagg tttggcccag cttctgtgct tcactgcccc
     5821 ggtcctcacc ttgaaggatc ccaggccctc ttgttgcttg ttctcagtgt tgctctatac
     5881 ctctatctcc tgctgtatgc tcagcagcag cccaaggact ctctaaaacc agggtgtgct
     5941 aggggctgga gagggctcag cgactaagat tgtgtgctac tgtcacggga gagctcaata
     6001 tagttctcct atgagagcta cactgggtag ctcccaacct cctgtgactc caattccagg
     6061 ggatccaacc ccgctggcct ctgtaagcac ccgcactcgt gtgtaccgtc acccctcctc
     6121 ctgaccccgc acaccacatt cccgtaattt aaaagagtgt gctgctttac tccacgggcc
     6181 cagtagtcgc catcacgtcg gctgggtctg cagtctatcc agcaaaagca agctggaaca
     6241 tttggtccag ctcagtgata agacactggc ctgctcggtc cagccaggcc ctgagtcctc
     6301 taaccctagc acaaccaaaa ccaggaaaaa ggggcccaag aggcttgaag ttttaaaact
     6361 ttcctttccc taaagtcact gtggctccct ccaaggtatg ccctggtcgt gaaggcccca
     6421 tcttctttgg tgatgagcag cacggctttg tgttcagcca caccttcttc atcaaagaca
     6481 gcctggccag aggcttccag cgctggtaca gcatcatcgc catcatgatg gatcggatct
     6541 acctcatcaa ctcctggccc ttcctgctgg ggaggatccg cggcatcatc agtgagctcc
     6601 aggccaaggc cttcaaggtg atgctcagag tgggaggggt gggggtggag ctctggcctg
     6661 tgggcatgga tggcacgcgc ccagcagctg tcctcttggc tctgcctctg ctgtgggccc
     6721 aggttgtagt ctcggcctca gtgttgtggt agggatgggt gaacctgcca gccaacctgt
     6781 gactcctggt ttcagctcac ccactactct ggtgctcatt cagcctgttg ccttggattt
     6841 ttgttctttt ggtttggttt gggttttttg tttttcttct ttttttttct gttgtttttt
     6901 gggtttttgt tttgttttgt ttttgagaca gggttcccct gtgtagccct ggctggcctg
     6961 aaactcactc tgtagaccag gctggccttg cattcagaga cctgcctgcc tctgcctccc
     7021 gagttaaagg cgagcactac cacccagcca cccagttgcc ctaattgccg ttcaacctct
     7081 gccttctttc ctgatgtctt caggacccag accgtctgtt agtcttgtct agacccccac
     7141 ctagggcgcc cctccagctg cagcagggca tgcaggcagg cctgggcatg cttgggtcat
     7201 agacccttag tttcagtggc ccactcgttc cttgtccatg ctgtgatagg tgaggcagcc
     7261 ggactgcatt agagtgtgac tggaaagcat aggaaccgct gaggagcagt tagtcacgtg
     7321 tccatcacct gcaagggggt gaacacccac tgggagtcca aggagccaag tggggcgagg
     7381 gggaaataca cggaaccctc tggatcagca gaaccattct gtgaggcatt gctagactgc
     7441 tgccaagaga gcagagcggg agaatcttgg aaggtggggt ggcagtaagg ctcggtggga
     7501 gccatccctg aagaggccct cttctccacc ctttgtattc aggtgtttga ggcagagcag
     7561 tttggatgtc cacagcgtgc ccagaggatg aacactgcct tcacgccctt cctgcaccag
     7621 aggaacggca acgctgcccg ctctctgacc tccttgacca gtgatgacaa cttgtgggcg
     7681 tgtctgcaca cttcctttgc ctggtaatga attctaggtc ttgacacctg tgagccatta
     7741 gaacaaccca aagtcaccca tctgcctccc agtggggcgg gcctgagtgc ctctgtaggt
     7801 ctgctccaac gcagagagtt ctcttgtctg tctcctcacc ggatggagac acttcgaggt
     7861 agagctcagt tcgcactgac acaaagacca tttctgtgtg aagaccatca agtctcctct
     7921 ctgaagatgg cagcaggcct ctcccgagcc ggtagctggt gctttgggct caggtctggg
     7981 aggttttgct ggggggcagc cgtcagctgg tgtctggagt gaaggtctgg tgtgcgggtg
     8041 cgctatgaac aaggagctcc tgttcctctg actgatgggt cttaggaaat agaacctgca
     8101 gcgcctcagc cccgaacctg cagcgcctca gcccctagtg tagcagggat gtattgcacc
     8161 ttctagagtt tgctgttgcc tatgaaccac atttgtcagc tgatgagcat ctaggctggg
     8221 tccgttcctt ggccattggg aatagagctg cagtaaatgg gtatttaagg atctctgctg
     8281 tggtgccttg gagtccttca ggaaatgccc gggaatggtg tagtgggtat ggatttggag
     8341 gaacctcccc actggtttcc aaggtggctg taccagctga cgtgctcccc cctgcccccc
     8401 aacagtacat acgtccctgt ttactttgtc ctcgccagca cctgttgttc ttggttttca
     8461 atttattata ctttatgtgt atgagtgctt tgcctatatg tatgtatgtg tcctacctgt
     8521 gtgcctagtg cctgtagtgc ctgtagtgcc tgtggaagtt taaacaaaag acaggaagaa
     8581 gagttggatc ctagttccag gacagccagg gctgcacaga aaaaccctgt ctcggaaaaa
     8641 caaaacaaca acaaccaaaa aagagttgga tcctatggga ctacagatgg ttgtgaaccc
     8701 tcaagtgggt gccgggaact gagcctgcat cctctgtaag agcatccagt gctcctaact
     8761 gctgagctgt ctctgtagcc acggcccttg gctttcctgg tgataggcat tctaactgat
     8821 gtgaaatgaa atgtaaggcg cataacgata ccacgatatc aacaagtttg tacaaaaaag
     8881 caggctggcg ccggaaccga agttcctatt ccgaagttcc tattctctag aaagtatagg
     8941 aacttcgaac cctttcccac accaccctcc acacttgccc caaacactgc caactatgta
     9001 ggaggaaggg gttgggacta acagaagaac ccgttgtggg gaagctgttg ggagggtcac
     9061 tttatgttct tgcccaaggt cagttgggtg gcctgcttct gatgaggtgg tcccaaggtc
     9121 tggggtagaa ggtgagaggg acaggccacc aaggtcagcc ccccccccct atcccatagg
     9181 agccaggtcc ctctcctgga caggaagact gaaggggaga tgccagagac tcagtgaagc
     9241 ctggggtacc ctattggagt ccttcaagga aacaaacttg gcctcaccag gcctcagcct
     9301 tggctcctcc tgggaactct actgcccttg ggatcccctt gtagttgtgg gttacatagg
     9361 aagggggacg ggattcccct tgactggcta gcctactctt ttcttcagtc ttctccatct
     9421 cctctcacct gtctctcgac cctttcccta ggatagactt ggaaaaagat aaggggagaa
     9481 aacaaatgca aacgaggcca gaaagatttt ggctgggcat tccttccgct agcttttatt
     9541 gggatcccct agtttgtgat aggcctttta gctacatctg ccaatccatc tcattttcac
     9601 acacacacac accactttcc ttctggtcag tgggcacatg tccagcctca agtttatatc
     9661 accaccccca atgcccaaca cttgtatggc cttgggcggg tcatcccccc ccccaccccc
     9721 agtatctgca acctcaagct agcttgggtg cgttggttgt ggataagtag ctagactcca
     9781 gcaaccagta acctctgccc tttctcctcc atgacaacca ggtcccaggt cccgaaaacc
     9841 aaagaagaag aaccctaaca aagaggacaa gcggcctcgc acagccttca ctgctgagca
     9901 gctccagagg ctcaaggctg agtttcagac caacaggtac ctgacagagc agcggcgcca
     9961 gagtctggca caggagctcg gtacccggaa gatctggact ctagagaatt ccgcccctct
    10021 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
    10081 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
    10141 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
    10201 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
    10261 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
    10321 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
    10381 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
    10441 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
    10501 tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg
    10561 ttttcctttg aaaaacacga tgataagctt gccacaacca tggaagatcc cgtcgtttta
    10621 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
    10681 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
    10741 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
    10801 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag
    10861 atgcacggtt acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg
    10921 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa
    10981 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat
    11041 ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt
    11101 gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg
    11161 agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac
    11221 gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt
    11281 aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt
    11341 gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc
    11401 gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta
    11461 cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg
    11521 gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt
    11581 ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt
    11641 cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg
    11701 atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat
    11761 tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat
    11821 gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc
    11881 tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg
    11941 agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg
    12001 tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc
    12061 gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc
    12121 ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc
    12181 ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa
    12241 tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg
    12301 gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat
    12361 tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc
    12421 acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta
    12481 tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc
    12541 ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat
    12601 gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc
    12661 gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc
    12721 gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc
    12781 gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg
    12841 ggtaataagc gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc
    12901 gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac
    12961 gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag
    13021 gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct
    13081 gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc
    13141 agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa
    13201 gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta
    13261 gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact
    13321 gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc
    13381 ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg
    13441 cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc
    13501 catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg
    13561 gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaattcca gctgagcgcc
    13621 ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg caggggggat
    13681 ctaagctcta gataagtaat gatcataatc agccatatca catctgtaga ggttttactt
    13741 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    13801 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    13861 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    13921 gtatcttatc atgtctggat ccggaataac ttcgtatagc atacattata cgaagttatg
    13981 tttaaacggc gcgccccgga attcgccttc tgcaggagcg tacagaaccc agggccctgg
    14041 cacccgtgca gaccctggcc caccccacct gggcgctcag tgcccaagag atgtccacac
    14101 ctaggatgtc ccgcggtggg tggggggccc gagagacggg caggccgggg gcaggcctgg
    14161 ccatgcgggg ccgaaccggg cactgcccag cgtgggcgcg ggggccacgg cgcgcgcccc
    14221 cagcccccgg gcccagcacc ccaaggcggc caacgccaaa actctccctc ctcctcttcc
    14281 tcaatctcgc tctcgctctt tttttttttc gcaaaaggag gggagagggg gtaaaaaaat
    14341 gctgcactgt gcggcgaagc cggtgagtga gcggcgcggg gccaatcagc gtgcgccgtt
    14401 ccgaaagttg ccttttatgg ctcgagcggc cgcggcggcg ccctataaaa cccagcggcg
    14461 cgacgcgcca ccaccgccga gaccgcgtcc gccccgcgag cacagagcct cgcctttgcc
    14521 gatcctctag agtcgagatc cgccgccacc atgattgaac aagatggatt gcacgcaggt
    14581 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
    14641 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
    14701 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
    14761 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
    14821 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
    14881 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
    14941 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
    15001 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
    15061 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
    15121 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
    15181 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
    15241 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
    15301 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
    15361 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
    15421 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    15481 agcgcgggga tctcatgctg gagttcttcg cccacccccc ggatctaagc tctagataag
    15541 taatgatcat aatcagccat atcacatctg tagaggtttt acttgcttta aaaaacctcc
    15601 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    15661 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    15721 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    15781 ggatccgggg gtaccgcgtc gagaagttcc tattccgaag ttcctattct ctagaaagta
    15841 taggaacttc gtcgagataa cttcgtatag catacattat acgaagttat gtcgagatat
    15901 ctagacccag ctttcttgta caaagtggtt gatatctcta tagtcgcagt aggcggattt
    15961 gtaatttctt cctctgacaa tggtctcggg tgcctttggt tgtgttttta cacctgttat
    16021 gtagacatgg ggacattacc tcacggagca gaactaaagt gttgtctgga gtgctactta
    16081 gtcaggattc ccttttgtct caaaggtgac ttctgaattg ggattaaaac caacagttcc
    16141 ttcaaacttg ccatactcca tttttctcat cccctgtagt ggaagaccaa actcctgtct
    16201 cacagaatgc ttcctcagtg ctggatgctg gggtgttggg gggtggtctt tctgtgagct
    16261 gggggttgag ccaagcgagg gcttactaaa gccccaggtt cagctttccc attggcagga
    16321 caccccatga ccggcattgt attcttctcc aggctcctga aggcatgcgg tagcaggctg
    16381 acagaaaagc tcttagaggg cgctcccaca gaggacaccc tggtccagat ggagaagctt
    16441 gctggtaagt ctgtttatct ctgctgggcc gactgtggtg gcagcagtgg ccctggcttg
    16501 tggctgccac agtgagtgaa gctggtccaa gggagtctgg atgtgaatga gcatgatggg
    16561 ccttggtgac aaggaaagtg ctcccgtgct cttggtgaca ttgctggttc caacacctct
    16621 tccttgattg tgcctgttcc caagtagatg agatggcgca acgcaattaa tgataacttc
    16681 gtatagcata cattatacga agttatggtc tgagctcgcc atcagttcat acctcagctg
    16741 gtctcctcag caattatgtt ttgcttctgt aaccttgaag agaagccttg tgaaacttgt
    16801 tagtttcagg aacttcctga gatttgtatc tcatgagcct ctctttggag ggaatgtttt
    16861 agttccgagg gaatcactgt gcaaccccat ccctacatcc atgatttaat ctctgcacat
    16921 ttcctttgcc ttgggtttag actcctgtgc taggacttcc tagatgatcc caatgcagga
    16981 ttgcagccct ctgatctgac tcggctgcca tctcgactgt ttgagttcag agttctggag
    17041 ttcaccagcc tgtcccattc gcccttttcc tctcccccag aagctgacag gtggaaaagc
    17101 aacagtcact tacagtacac ggtgcacagt ttgttactaa cagtgctgcc ggctcctcca
    17161 ctgtaagcag atggagttct ttctaggttg tcactaggtg tcactcctat tataaggttc
    17221 caccatgagg actaatggaa agaaaacatt tgtgtcttgt ctgggtagag ggagctgggg
    17281 tgaaatggtg tgtggagccc agactggccc ctggccgagc agtgctgata aggtcacgct
    17341 gtctgtctgc tctgctgagc tttccagcag ttgagcagag ccagcatctt tcaggaagag
    17401 cttctaagat gtctcttcta cccaagtctt cctcccctca ccccaggtgc ctagtcagta
    17461 gcatatagtt attggacctc tctgggatac gataggcgtt ggtacagcca taggtaccct
    17521 gaggcagaag tggcccagaa ccaaactgta gcgtcgaagg ggccgctcac tccatacctg
    17581 ccctgtctgt cctgtagact tggaggaaga atcagaaagt tgggacaatt ctgaggctga
    17641 ggaggaggag aaagctcctg ttacaccaga gggtgctgaa gggcgagagc tgaccagttg
    17701 cccaacagag tcatcctttc tctcagcctg tgggagctgg cagcccccaa agcttaccgg
    17761 cttcaagtct cttcgacaca tgagacaggt aagcaaaggg ggcggtggtc caggccactc
    17821 tgccgggtac cagtcctcag ggtctgagct ttttccacgt ccgtgggcag gggagacact
    17881 gggaacatct gagggtctgg gtgtatgact gggggctcag aggacatcag cctgtcttag
    17941 taggatcagc attcttgtgt gtttgtatgc attcacgtgt gcgtgtttgt gtatgcgcat
    18001 gtgtagagac agaccagagt gtcattctgg gtgctgttga tattgtgctt tgagacggtg
    18061 tccctcactg gcctgggact tgtccagaat gctatttggc cggcttgtgt gccccaggga
    18121 tctgcctccc aagccctctc agctgtggga taacaagcac atgccaccgt gctttgcctg
    18181 ccaccttaac cttgagtcct ggagtataag ctcaagtccc ccatactttg aaggtaagtg
    18241 tctgtccagc ccagcatctg tttgtaaggt ttcctgaggt cctctaacct tgccagcttg
    18301 gaacctcctg aaggtgtcaa aaaagcacta ttagctgttt gtgtgcaggg ctagacctta
    18361 aagtaatggt caatcctaga ataaatcggg cgtatataca catgcctcct gcaaatggaa
    18421 atagtcttac gagattgaac atggtttgct gggtcagatg agaagcgagg gtcactccag
    18481 gtgaaggcac actgtcccct cttcacaccg ctgtctctct ttgggatagg gtctcatgag
    18541 gtcactatct taggcaggct ctgaagtccg tagcttacca agtgttggga ttacaggcac
    18601 gtgcaagcat gctggcttaa ttttttaact atatttcttt ttattttaaa tctagaattt
    18661 taaaatttta tttaccgtta ctactatgtg tggaggtaca tacatgtgtg ccagaggcca
    18721 atttttgtag catctctcct tcctgcttta tgtgggctct gactggacac ctgtctcaca
    18781 tggaaaatcc ctctcccaac ctgttgagcc atctctctca ccttctttat tttatttatt
    18841 ttaatgagta ttaatttgac cagccaagaa ttatttctgt tttggagact cgagagtcta
    18901 gtgagatgac ccccagggta agggtgttgg caaccataaa gacctaagtt cgatccccag
    18961 gcacgtggat tgagccctag agttcagctt gggagacatt tgttctccag tgctgagctg
    19021 tactggcttc gccaattctt ctgaggttaa cagtagcagg gattgcgatt cctgctacag
    19081 atgggggtac tcagcattgt gttttgtaaa gtgtgcatgt tgtagcctcg gttttgttag
    19141 tttcgttctg tttcagcctc atgtttgacc tcaagaagca acaggctagc tcctgactgt
    19201 cagtgttgca ggaattactg acggggggtg gtactgggag cggggtgggg gggaggtggg
    19261 ggtaggaggg aggctatcct gtcacggcct cagcatctga ggaactgttg ataaagaagg
    19321 tcacctcagc tggcttgggc aagtgccagg atgtgggtgc atcctggtgc tggaggcaga
    19381 gccctcgtct tccgggtgcc cgggtgccag tgtgaggagc tagacgttca ggtccaagta
    19441 ttccagcatg acagcctgtt agagaggccg agggctgcca gagttagaca ccaagctcaa
    19501 aatcacagag acccgcctgc ctctgcctcc ctggtgctgg aattaaaggt gtgcgccgcc
    19561 actgcccacc tggcttgctt catacttttc atagtgtagg gaaggtttgt taagaatgta
    19621 ataccagccg ggcatggtag ggcatgcctt taatcccagc actcaggagg tagaggcagg
    19681 cggatttctg agttcgaggc cagcctggtc tacaaagtga gttccaggac agtcagggct
    19741 atacagagaa accctatctc gaaaacaaac aaacaaaaag aatgtaatac ctttgaacct
    19801 ggtgacgact tctgtcccct gtcagcctct agcaggtcac cagtatccct agaaggagaa
    19861 ttaacgttcc ctttgctgac tgtggtcttc tttattgtag agattgtagt cgctgactgt
    19921 gtgaggctag atgaactcct acaatatacc taaagagcta cgtgcttgct gtgttccagg
    19981 tcttgggtgc tccatccttc cgtatgttgg cttggcatgt cctcatgggg aatcaggtga
    20041 tctggaaaag cagagatgtg aacctggtcc attcagcgtt tgaagtcctc cgggtgagaa
    20101 ccttcccctg tgtgcgcgtg tttgacttgg ggcagactct caggggcatc accgggcaga
    20161 acctcagaaa ctggttttct ttgcctggtg aacatggcag gcattgacac tcatggaagg
    20221 agcctgtatc atcaccattg acagctacac tgtctcccct tcctcagtct gaaactcttc
    20281 tgggaagtta tctgggcaag ctctggcact ggctgatgct ccgtggctgt tctcaagttg
    20341 ggtcgtggtg tgggagcttc tcatggcgcg tgcgctggcc gccggaaccc acaagtgcct
    20401 ctctgtgcag tgagggaagg ggcagcgcat gtcagcagca ttgatctgtg actgcccccc
    20461 caggaccaga attggggtca tctggggctg ggagacaatg gacacactga ggagggaggc
    20521 tgtgtgagct gcagagggtg catttgtgct ggattctgag ccgccgtgtt gtccacagac
    20581 catgctgcct gtgggctgtg tccgcatcat cccttacagc agccagtatg aggaggccta
    20641 tcgctgcaac ttcctggggc tcagccctcc cgtgcctatc cctgcccatg ttctggcctc
    20701 aggtaagtgt gtgagacaca tgctgcctgc tgctctgaaa ggctcccgaa tccagcccag
    20761 gggtgcaccc ggatccaaat cacagacttt agggcgaagc agtactctgc agcgccagcg
    20821 aggacggcct gggcttgccc ggcgcccctg gagctgtgcc ttctgtttgc agtaggactg
    20881 gtctgtgccg tggtgtttgg ctgttaagtt cccgtactgt gtgtctgtgt ggcctgagcc
    20941 ctccaggcag tttccatctc tggcagaggg tctcagtctc cctgatgctc tgacccttta
    21001 attccgttcc tcatgttgtg gtgacccccc aaccatcaaa ttacttttgt tgctacttca
    21061 caactgtaat tttgctactt gttaggaatt gtaatataag tatttgtgtt ttccagtggt
    21121 ctcaggagac tcctgtgaaa aagggtagtt caaccccagg gggggtcaca acccctagga
    21181 tgagaaccac tgatctacag gctcaagcag tagtgagacc atttacagag gactgcttag
    21241 gaaaacttat gtcatttatg ttctagtt
//