LOCUS       KO-first_condition_ready_35697_MGI:2442058        22014 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Nipa1 targeting project(s): 35697
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 44768
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..8676
                     /note="5 arm"
     misc_feature    8704..8728
                     /note="B1 Gateway"
     misc_feature    8741..8788
                     /note="Frt"
     intron          8789..9663
                     /note="mouse En2 intron"
     misc_feature    9661..9663
                     /note="Splice Acceptor"
     exon            9664..9836
                     /note="mouse En2 exon"
     misc_feature    9848..10441
                     /note="ECMV IRES"
     gene            10442..13502
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13541..13784
                     /note="SV40 polyadenylation site"
     misc_feature    13788..13821
                     /note="loxP"
     promoter        13848..14367
                     /note="human beta actin promoter"
     gene            14393..15187
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15387..15628
                     /note="SV40 polyadenylation site"
     misc_feature    15645..15692
                     /note="Frt"
     misc_feature    15699..15732
                     /note="loxP"
     misc_feature    9678..9755
                     /note="FCHK std_gpr"
     misc_feature    15748..15768
                     /note="B2 Gateway"
     misc_feature    8677..15798
                     /note="Synthetic Cassette"
     exon            17187..17277
                     /db_xref="ENSEMBL:ENSMUSE00000350237"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000350237"
     exon            16148..16195
                     /db_xref="ENSEMBL:ENSMUSE00000361177"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 2 ENSMUSE00000361177"
     misc_feature    15799..17430
                     /note="Critical Region"
     misc_feature    17454..17487
                     /note="Downstream LoxP"
     misc_feature    17431..17510
                     /note="synthetic loxP region"
     misc_feature    17511..22014
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggacaccag ttttaacatc caattcttct agatttactc gttcttttgg
     4321 cagagggttg aactacttgc ttaaattgtt tctctgtctt aggattaagt cttgcaatat
     4381 ctctgccctg agattctctt acttagatgg aatgctccct gcctcttaag ttattaggtg
     4441 actagtttca gagaaaaagc ttgcttgaat gcatgactat ggcttttgct tttagactgc
     4501 catttaatgc aatggcattc atgttttcct tagacctcac ccttaaggtg atgtcttacc
     4561 tccatttcct taggctagtg ttgcctacag taaatgcata ggagaatttg agaaaagtag
     4621 cctatgagag aacacaagta aataaaagcc taatggtagg aaaatgggga gtagagtcag
     4681 ttaggagtca cagatggtgt ctgcatgtga agaagacagg tgttgagaac cttcacgggc
     4741 cagggttgaa tcctgctcct ccccaatgca gggtcaggag agggtcctgg agaattatcc
     4801 caagaggagg gtgcagaggg tgagagctgc tgtggtgtgg ttacgagtcc catatgaatt
     4861 tgccatacca tgtcatgccc agtcttcaga aagctaggag gaatagtgga tgtggacagt
     4921 gaatgccagt ggttttaaga gaaagtctgc gtctccaaca ctacaacact atgccaaacc
     4981 catacttgtt gccactttgg ctggtcatgt gacatcactg tctttgttgc ccatgtgacc
     5041 agttatgcct ttgggtgaca ggcagctcca tgtgtgtata actctgggag ggtatcttat
     5101 aagttggctg gggtccacgt gggttcctat gaacttccaa gtttgaaggt agactctccc
     5161 agataaacac ttaccagttt aatcttagaa aatgcttcta tcagatttgc ctactagcaa
     5221 gtctgtggaa cattttcctg aatgatgatt gatgtaagat ggcccggttc accatggggc
     5281 tgccatctct gggcaagcag ccctgagttg tataagaaag caggctcagt aagcgatgag
     5341 gagtaagcca gtaagcagtg tttctccatg acttctgctt cagttcttgc cttcaggttc
     5401 ctgctttgac ttttctcaat gatgctctgt gtgtgattgg gaaatgttag ccaaaaaagc
     5461 tttcctttca tgttggtttt ggccatggtc tttattatat cattaggaac taagcctggg
     5521 caccttggaa gggaaagatt aaaaaagtgt ttgaatatgg gttcctcagc ctttgatcag
     5581 gcattccatg ggaacttccc atcgtttcct tgtgtttgag gaaggagaga attatgctgt
     5641 ggtaggtaat cttgttaagg atgagaacat ttgaaagcct ttcctgcttc cctcctagct
     5701 gcctgatatg acagtggtta taggtgcaca tgtgacttca gagcacagct gctaaaattg
     5761 tcaggttctt gtgtctgcaa acagtgattt ggacaaagac acatagaatg ggaaaagata
     5821 gtttattaga tgaaagaaat aagcaaactt ggaaatacag cagagctagg tgaggtctag
     5881 gtcccagaag agatcaaggt tgcagctggg cagtggtggt gcatgccttt gatctcagca
     5941 cttgggaggc agaggcaggc gggtttctga gttcgaggcc agcctggtct acagagtgag
     6001 ttccaggtca gccagggcta cacagagaaa ccctctctcg aaaaaccaaa aaacaaaaca
     6061 aaagagagaa agagacagac caaggttggg catctgtcac cattaatggg tcacttacat
     6121 attgtgcacc ttttctgaca ttatggtttt tatttcatat acccagtgtt caggaacgtt
     6181 tccacttttc ccgtaatatt ggctgctttt atgtgtttat cctggcgcct ttaggatctg
     6241 atcctctcct tggtcatgca tcttgttaat tcacaggatt cctgctgttg ggcaacagtt
     6301 gcgctgtggg tgatcgttgc tatcagctcc tttgctactt gatgtcacca aattcctttc
     6361 agggaacaac ttaatggaga gtttattcca taagccttac agattaagaa ggaatccagt
     6421 ccgttatgtt acaaaagaca aggctgcggg tgcgtgaggc agctggtcac agtctgcagt
     6481 caggaggcag agagggctct actttctaaa gttctccaac ctttcaacag cacaagaagt
     6541 tggggactag gtttccaaac acatgatctt ctggggcaca tttcatattc aaagcacagc
     6601 attccattcc cggccatttt atagtgcaag acggatttgc tccaacgtca gaagtcctca
     6661 tgagtgctca gatgactcat tggctgaaag tgtattcagt gcaagcctag gacctgagtg
     6721 aggttaatct gtgggactta cggtgagaga agatacctga cttctggaag ttgtctgttg
     6781 acctttacag acatacatgc atatgcgtgc acacacacac acacacacac acacacacaa
     6841 gcatatattt tcttcttgct cagggatctg cagtttccca gcacctgccg actgacacag
     6901 cctggtgact cctaactaaa ctgactcccc actttccagc cattagactt tatatacctt
     6961 cgttgtgttg tcgattacct ttctcaaatt cccctgacca ttttactgta gacagcacac
     7021 taacctaagg atatggagat aatttcccag gtaacaggat gtcactgcat aaactggcaa
     7081 cacgaccact caatgatatg ggtaaaggca aagtttttag tgtagctatg agggagagaa
     7141 cagccagagg catcaggaag agtccagagc agagagagaa agtagcaggc tgaccatggc
     7201 cagcaggctg gacctggtca ggagacaagc aaaggcagag agcagagaga gaggcagagt
     7261 aggagtgggg gagacagaga ggagggccga ggacaggcag aagagggagc acagcccaaa
     7321 gagcaggtta tatggagaat gaatggctgg gggggggggg ggcgagggga cgctcctgaa
     7381 tggtgcaagt ttaggatggg gatggagaag agtagatgga ctctgaaatg tgtaataagc
     7441 acgcagatac tgagggagcc tggaggctag cctgcatttt gttatgctga taaacaccac
     7501 ccacagagag ccctttgtcc ttctgccagg gataagggaa atgactagtt ttggtagagg
     7561 ggaactggct tcacagttcc tgaggaatgc tgctggcttt tgtctaacca ctagaatgtg
     7621 aggaaataga gttccctctg gacttgataa tcaccttaaa gtcggggtat atggaaaata
     7681 aatagccctg cattcacaca gcaatgataa atagaattta tttcccagtg aggcaggaaa
     7741 ataggatgga gagtcccaag aatatttttt aggttgtgac ttatttagcc aaggaacacc
     7801 aacctatgag caagttgttt gtcagcctgg gttggttgag aaaaaacaaa acaacccatc
     7861 caaaccccac tttactactt tcagtggctt ctcgcccatt gatagactaa agattactaa
     7921 tgagctgagt aagcaagtta acactcactc ctttttgctt agttttcatg tgaggagttc
     7981 tctaagtgtc attgtgaatc tcttacttgg taatgtatat aataactttt tttaaaggtt
     8041 gaaaaatttt aattacacac tgtaagagtc tataattcac caaagaatga tacccaggac
     8101 tcaaatagta tgtaaacgca gactgtttta ttctgcacaa gtccagcatg ctggggtctc
     8161 ccattaccaa gatagagaga caaccaagtg tgttcacagg ctaccaagtg tgctcaaagg
     8221 cctgatttaa aacatagtag gacattctag ggtagttgac ccttatcttc cattactggg
     8281 acatgggggc tggaaaccat tgctgacaaa gtaactgagg aagtctggaa actgctgctg
     8341 acccattgtc cttgcctcat taggccaggt gatggggcag cttctgaggc ctagacttgc
     8401 ctggaattgc ccagttctta gaaacaaaga tttaggctta gtcttcctaa ctgacaattt
     8461 gaagcctgtt atggaatcag cctggccttc tcagcatgtg ggtggtggtg gtggtggtgg
     8521 tggtggtggt ggtggtggtg gtggtagtgg tggtggtgat tgtggtggta ggtggtgtac
     8581 atgtgtcttc agaggctgga ctgaagttat agacagttgt gagctgcctg gtgtggatgc
     8641 cagggattga acttgggtct tctgcgaggg tagtaaaagg cgcataacga taccacgata
     8701 tcaacaagtt tgtacaaaaa agcaggctgg cgccggaacc gaagttccta ttccgaagtt
     8761 cctattctct agaaagtata ggaacttcga accctttccc acaccaccct ccacacttgc
     8821 cccaaacact gccaactatg taggaggaag gggttgggac taacagaaga acccgttgtg
     8881 gggaagctgt tgggagggtc actttatgtt cttgcccaag gtcagttggg tggcctgctt
     8941 ctgatgaggt ggtcccaagg tctggggtag aaggtgagag ggacaggcca ccaaggtcag
     9001 cccccccccc ctatcccata ggagccaggt ccctctcctg gacaggaaga ctgaagggga
     9061 gatgccagag actcagtgaa gcctggggta ccctattgga gtccttcaag gaaacaaact
     9121 tggcctcacc aggcctcagc cttggctcct cctgggaact ctactgccct tgggatcccc
     9181 ttgtagttgt gggttacata ggaaggggga cgggattccc cttgactggc tagcctactc
     9241 ttttcttcag tcttctccat ctcctctcac ctgtctctcg accctttccc taggatagac
     9301 ttggaaaaag ataaggggag aaaacaaatg caaacgaggc cagaaagatt ttggctgggc
     9361 attccttccg ctagctttta ttgggatccc ctagtttgtg ataggccttt tagctacatc
     9421 tgccaatcca tctcattttc acacacacac acaccacttt ccttctggtc agtgggcaca
     9481 tgtccagcct caagtttata tcaccacccc caatgcccaa cacttgtatg gccttgggcg
     9541 ggtcatcccc ccccccaccc ccagtatctg caacctcaag ctagcttggg tgcgttggtt
     9601 gtggataagt agctagactc cagcaaccag taacctctgc cctttctcct ccatgacaac
     9661 caggtcccag gtcccgaaaa ccaaagaaga agaaccctaa caaagaggac aagcggcctc
     9721 gcacagcctt cactgctgag cagctccaga ggctcaaggc tgagtttcag accaacaggt
     9781 acctgacaga gcagcggcgc cagagtctgg cacaggagct cggtacccgg aagatctgga
     9841 ctctagagaa ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     9901 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     9961 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
    10021 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
    10081 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
    10141 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
    10201 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
    10261 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
    10321 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc
    10381 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac
    10441 catggaagat cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
    10501 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    10561 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
    10621 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt
    10681 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac
    10741 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc
    10801 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga
    10861 tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga
    10921 cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct
    10981 cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg
    11041 gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga
    11101 tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt
    11161 tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga
    11221 aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg
    11281 ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga
    11341 aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga
    11401 agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct
    11461 gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg
    11521 tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt
    11581 taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg
    11641 ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa
    11701 tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt
    11761 gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca
    11821 cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt
    11881 gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc
    11941 gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct
    12001 ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa
    12061 cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg
    12121 cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc
    12181 gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa
    12241 cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca
    12301 gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt
    12361 ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc
    12421 aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga
    12481 actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa
    12541 cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga
    12601 aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga
    12661 aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt
    12721 tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt
    12781 cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa
    12841 cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca
    12901 gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca
    12961 tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat
    13021 ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct
    13081 gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga
    13141 aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga
    13201 catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt
    13261 gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca
    13321 acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct
    13381 gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc
    13441 ggcggaattc cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata
    13501 ataataaccg ggcagggggg atctaagctc tagataagta atgatcataa tcagccatat
    13561 cacatctgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
    13621 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
    13681 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    13741 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccggaata acttcgtata
    13801 gcatacatta tacgaagtta tgtttaaacg gcgcgccccg gaattcgcct tctgcaggag
    13861 cgtacagaac ccagggccct ggcacccgtg cagaccctgg cccaccccac ctgggcgctc
    13921 agtgcccaag agatgtccac acctaggatg tcccgcggtg ggtggggggc ccgagagacg
    13981 ggcaggccgg gggcaggcct ggccatgcgg ggccgaaccg ggcactgccc agcgtgggcg
    14041 cgggggccac ggcgcgcgcc cccagccccc gggcccagca ccccaaggcg gccaacgcca
    14101 aaactctccc tcctcctctt cctcaatctc gctctcgctc tttttttttt tcgcaaaagg
    14161 aggggagagg gggtaaaaaa atgctgcact gtgcggcgaa gccggtgagt gagcggcgcg
    14221 gggccaatca gcgtgcgccg ttccgaaagt tgccttttat ggctcgagcg gccgcggcgg
    14281 cgccctataa aacccagcgg cgcgacgcgc caccaccgcc gagaccgcgt ccgccccgcg
    14341 agcacagagc ctcgcctttg ccgatcctct agagtcgaga tccgccgcca ccatgattga
    14401 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
    14461 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
    14521 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
    14581 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
    14641 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
    14701 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
    14761 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
    14821 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
    14881 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
    14941 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
    15001 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
    15061 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
    15121 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
    15181 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
    15241 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    15301 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    15361 ccggatctaa gctctagata agtaatgatc ataatcagcc atatcacatc tgtagaggtt
    15421 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    15481 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    15541 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    15601 atcaatgtat cttatcatgt ctggatccgg gggtaccgcg tcgagaagtt cctattccga
    15661 agttcctatt ctctagaaag tataggaact tcgtcgagat aacttcgtat agcatacatt
    15721 atacgaagtt atgtcgagat atctagaccc agctttcttg tacaaagtgg ttgatatctc
    15781 tatagtcgca gtaggcggcg gaggctctga aatcatatcc tagtgcccat gtaggaaatg
    15841 ataggaattt gtcataaata tctgtaactt catagcacaa acaggtaatt tgctagggct
    15901 tgctgtgcaa ttgctgaggc tctgagttca tatcctagca tccatgtaga aatcttggaa
    15961 tgcgtcatga gtatctgtaa ctccagagca ccaacagatg aaatgctagg gcgtgatggc
    16021 ccccagtcta ataaatccca tcagcctata gacatgtgta tatacatcat aaacacacac
    16081 acacacacac acacacacac agcactaaat gagcttgctt tcctgatatt ctcacctttt
    16141 tttataggta cttcctacct aacagacatt gtgtggtggg ctggcacaat tgcaagtaag
    16201 tggtttatat agtagcttac agtcttctta tatttttttc taaagttatc acatgggtgg
    16261 ggatggagag ggcaagatga gtgtggcttt gaaagtcctc ccatgcttct actctctgca
    16321 cttcagattc actctctttt cctgttgtcc ttctctttgc tgaaagagaa tagatgggaa
    16381 agtgtggaat ggctgacctg ggagaaggtg gcaggggctc cagcggcaga ccaaagatca
    16441 tgttctataa cttaaataat aaactgtgat ttttttaaaa taatcaggtg gtttgtcaca
    16501 tatggccctt taagcaaagt gctacctcag tgaggctgat tgtgctcatt ggggaggccc
    16561 cttttaagct ctgatggagt agcacacctc ggggtcctac agcccagctc tggacagcat
    16621 ttcaggctgg agcaggtcca aggtcacttg aaggctgtta gccccacttg tctaggaaag
    16681 attggtcatt gggtataaag tggcagtcag aaacagtatg gtaccttggt gtacttctat
    16741 agtgtacaca gaaactactg tgtgtatcac aaaaataggt taaaggtttt tgactgttgg
    16801 caccataaat gaaaaatgtt tgcgcacatg catgtgccca agtactacat ggtgccagtg
    16861 ttctgctttt atggtacatg tctgccataa ataaataaat aagtttaaaa tgtaaaagtt
    16921 gaaatgtttt aagatagcgt gtagagagcg tgaatgtatt cagtgctgta ccggccactg
    16981 tgatagcgct ggccgtttcc agagagtgga gactacttct tagaccagtg aagttctgtc
    17041 attgggatct gcgagcatgt acatttgctt cctcagaagt ggggcagaag aagccctttc
    17101 aggacagaat cttgcaaaat cttgattggt gataaaaaaa aagggggggg ggactttgta
    17161 atgtaacctt gtgattctct tgacagtggc tgtcggccag attggaaact ttctagctta
    17221 cactgcggtc cctacagtcc tggtgactcc cttaggtgcc cttggagtac cgtttgggtg
    17281 agaactgaca ttctgtttta atatttaata tttagtttac ataggaaaaa aacctttcat
    17341 tgtaaatgtt tctgtttaaa aaaataaagt agttgatgac gatagatctt ttgtacttag
    17401 aattcatatt ttcattgtaa ccctagcaat gagatggcgc aacgcaatta atgataactt
    17461 cgtatagcat acattatacg aagttatggt ctgagctcgc catcagttca ctctgaaaaa
    17521 acttcataga acctagagtc ttcctcatac cacagtgtgt tattgtcaga aatctttctg
    17581 taaaatgtca gctcgtgttt tatgaggatg gttagcatcc tctgctagac cagcgttctc
    17641 ccacctcatt ccagtaagtt accgaacaaa agctgggcgc gtgacccatg ggcatcagtg
    17701 tacgtggcca agggtttgtc atcacagtcc ttcagacaga taccgagtaa tgagcgggaa
    17761 ctctgggctt tactctgtca gggtgatcca atccaaactg gaacttggct aactccaagc
    17821 tggcgtttca tggtttgaaa tatctgcctg tcacatggag tcatggctcc agggaccagg
    17881 aatatgatgt ggagaggagt aaagacttag agtgacagct gtcaaaaatt accctcttta
    17941 ttggcttggt caccaaggtc attttttctt gctggaacaa aatggctagg aatggataat
    18001 ttgtttttta agaagtaagg ttttaatttg ggttcataat tctagaggct acaaagtaca
    18061 agactgagta gcctccctct ttttttattt ttctttcttg ttttcttttg agctgtagcc
    18121 ctggctggcc tggcactcct tatctagacc aggctattct tgaggtagtc tgtttctacc
    18181 tcaggtgcta agataaaagt tacatagcac cacatctgga taaagatttt ttttttattt
    18241 aattttattt gttttttttt taacaattcc ttttattttt aatgatgtgt atatgtgtgg
    18301 tctgtgtgtg ggtatgtata catggaagtg caggctccct ggaagtccag aagagggcat
    18361 tggatctcct ggagttagag ttaggggctg tcgtgaagtg cctgatgtcg gtcagtgctg
    18421 atgtcagaac acagaactga gcttgggttt tctgaacagt ctgcatgtgc tcttcccaac
    18481 tgagctatgt ctccatggca acccccttca tgaggctcac aaactaggcc gagggagcca
    18541 cagaactgca cgttctaaca gttggttttg tggtaacaaa tccagtcctg ggagagctgt
    18601 actaatccca agctttccct ccctctttct cctttttcaa gacagggttt ctctgtgtag
    18661 ccagaacttg ccctgtagag caagttggcc tcaaacgcaa agagctctac ccgcccgtgc
    18721 ctccggagtg cagggcttga tccaccccca cctgcttcag taatcgcttt tagtgatgtg
    18781 atcactattt agaactgcaa atcaaatttc agcattagta tctatggaga caaaccacat
    18841 ccaagcgctt ggcttactag aaaaaaaaat ttattattat tatttttttt ttgagacagg
    18901 gtttctctgt atagccctga ctgtcctgga actcactttg tagactaggc ctcgaactca
    18961 gaaatccacc tgcctctgcc tcccaaatgt tgggattaaa gacgtgcacc accactgccc
    19021 agctccccca aaattttttt ttaaagattt atttatttat tttatgtata aaaagtacac
    19081 tgtagctgtc ttcagacaca ccagaaaagg gcatcagatc ccattacaga gggttgtgag
    19141 ccaccatgtg gttgctggga attgaactct ggacctctgg aagaataatc agtgctctta
    19201 acctctgagc catctctcca gccctagaaa atgtttttaa tagaccttca ctaactgtat
    19261 caacatagcc tgctacaaag atgtgggtgt ctgttgtacc tttgaagaca ctaataataa
    19321 acagactagg atagaagaga agtaagagct gtttcatctc gtgcatatgt ggactgcagc
    19381 tttatatcag acagacctcc atccttacag agaagagtgt gataaatata ttctacctta
    19441 aattcacttt aaaattgtgt gctctagggg ctggagaggt gactcagcag gtaagagcac
    19501 ttcttgccct ttcagagtat ttgagttcag ttctgagcag tgttccaaca ttgtgtctct
    19561 gaaccacctg taagtccagt ttcatggtac ctttagccct gttctgctgt ctgatggcac
    19621 tgcactcaca tggtgcacag gcatacattc aggcaaacat tcatacacat aaaataaaaa
    19681 caagtctcaa aaaaagattc tgtggtctaa aaacctcaac tctttttttt ccacatgtaa
    19741 ctttgcaaat acgttttttt tttctccact gttcttgtcc acacatgtgc ttggtattaa
    19801 tgttgcttga tgtcactttg tattttgggt gcccctgaaa gtctggtcgg ggcaggaatg
    19861 gtgcttatgc ctggaatcat tcctcagttt tcccagtttg tacccatgga agataagctg
    19921 acttcatgat ctttgaacat ggcacatgta atatgtaagc caatcttcac gcctgttata
    19981 attaagtaac ctactgctcc aattttgcaa gaggtgctat ttattcaccc agtgaagtat
    20041 actttttttt ctgctgtggt cttatggata aaacacagaa ctatgtgcat gcttcaagca
    20101 atacactatt gagctttaaa aaaaaataga gaaatcaagg caaaatagaa gaaagcagag
    20161 atagctaagt atgtgtgtgt gtgtggatta taagacattc tgttgataat gagtgaattg
    20221 ctgaatttat ttttttaatt tattttttat taggtatttt cctcgtttac attttcaatg
    20281 ctatcccaaa agtcccccat acccaccccc ccatccccta cccacccact cccccttttt
    20341 ggccctgggg ttcccctgta ctggggcata taaagtttgc aagtccaatg ggcctctctt
    20401 tgcagtgatg gccgactagg ccatcttttg atacatataa ttgctgaatt tataaagaat
    20461 aggaaataat atggcactgg gaagaaaatc aatctatatt tctgaataga gcagagagcc
    20521 ccgtcaaata gctctatttt aatacataac agtttaatat atgtaaaata ggcattacaa
    20581 tatactttaa cagatgtttt aaaaacttac ttggaaataa tttccaaatt atggaaaggg
    20641 tataggatta agaatcatcc taattagtgc cccaggccct cacacagaca cacctcttag
    20701 ggccttttta gcttactttc tctgtccttg actttcttga atcaagttct taaccttggc
    20761 agtattgcca tttgaggtca gatatttctt tgctgggtat attaaaggaa gctgttctgt
    20821 agactgtagg ttgtttagca gtgtccctgg cctctgctta ctacatgccc taagcattga
    20881 tccccttccc aagtagctct agacatacaa aaaaaaacaa tccatatgtt cagcagaagg
    20941 acacaggtcc tgttggaggc acttgccaca aagccaaatg actcatacat ctgacatgta
    21001 ggccctctcc ataaaatatc tgtaaataaa aaggaagaca tagtatattt tctaaataat
    21061 aacatatgag tattttagaa aagtgatcta ccagacaaag cagattgtac aataacaaca
    21121 aaatcttact gagggacatt ttatgataaa atggtcactt cttctggaaa atatagacag
    21181 ttaaatgcac aaaaagacaa cttcagaata catgtgacca aatttagaag aaaaaaaaag
    21241 tgagagaaaa tagagctggc tccatggttg agagcactgg ctgttcttca gaagactcag
    21301 tttggttccc agcacccaca ccgtaactcc cagctgtccc tattctagcc tcagggcctc
    21361 cacagctact aggcacgcat gtgatacaca ggcatacgtg caggcacaaa cctgttgaca
    21421 gtaaaacaag taggtaatac atacttaata tctgtgacga taatagaggg tgtgaatgtg
    21481 atgacagcac gtgacataga tggggtcagg agggccactt tggtgactgc atcggtgcct
    21541 ttgtaagaag gaaagacctg agctcacgca ttcgcttcac cccgccacat gatctcctcc
    21601 actaggccgt gggagtcaaa tgctaagcag attccagtgt tctacccttg gatcctcagc
    21661 ctacagaatg gagctaaata aacctctgtt ctttgtaagt tacctagttt gggaatgctt
    21721 acttatagca acagcaatga actaagataa gactgtaatt actaagacca gaaataaaat
    21781 agggacattt ctacaactat gtagaaatga aaataattat atggcatcag attaggtaat
    21841 ttaggagaaa tggacacatt cctagagagc ctactgaaac tgtttcaaga aaaaaaaaat
    21901 acaaatttct aattcttttt ttttttaaat agctctgaaa aagaaaagca aggaacaaaa
    21961 atattcactg attatttcta ttgtttggaa aatttatgta tgcatgcaat gcat
//