LOCUS       KO-first_condition_ready_38888_MGI:2388285        21402 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Pkn3 targeting project(s): 38888
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 51140
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     exon            4573..4801
                     /db_xref="ENSEMBL:ENSMUSE00001256245"
                     /rank="2"
                     /note="ENSMUSE00001256245"
     exon            4965..5110
                     /db_xref="ENSEMBL:ENSMUSE00000888626"
                     /rank="3"
                     /note="ENSMUSE00000888626"
     exon            5614..5725
                     /db_xref="ENSEMBL:ENSMUSE00000162749"
                     /rank="4"
                     /note="ENSMUSE00000162749"
     exon            5795..5922
                     /db_xref="ENSEMBL:ENSMUSE00001252493"
                     /rank="5"
                     /note="ENSMUSE00001252493"
     exon            6092..6275
                     /db_xref="ENSEMBL:ENSMUSE00001257488"
                     /rank="6"
                     /note="ENSMUSE00001257488"
     misc_feature    4215..7377
                     /note="5 arm"
     misc_feature    7405..7429
                     /note="B1 Gateway"
     misc_feature    7442..7489
                     /note="Frt"
     intron          7490..8364
                     /note="mouse En2 intron"
     misc_feature    8362..8364
                     /note="Splice Acceptor"
     exon            8365..8537
                     /note="mouse En2 exon"
     misc_feature    8549..9142
                     /note="ECMV IRES"
     gene            9143..12203
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    12242..12485
                     /note="SV40 polyadenylation site"
     misc_feature    12489..12522
                     /note="loxP"
     promoter        12549..13068
                     /note="human beta actin promoter"
     gene            13094..13888
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    14088..14329
                     /note="SV40 polyadenylation site"
     misc_feature    14346..14393
                     /note="Frt"
     misc_feature    14400..14433
                     /note="loxP"
     misc_feature    8379..8456
                     /note="FCHK std_gpr"
     misc_feature    14449..14469
                     /note="B2 Gateway"
     misc_feature    7378..14499
                     /note="Synthetic Cassette"
     exon            14895..15041
                     /db_xref="ENSEMBL:ENSMUSE00001266857"
                     /type="targeted"
                     /rank="7"
                     /note="target exon 1 ENSMUSE00001266857"
     exon            15120..15229
                     /db_xref="ENSEMBL:ENSMUSE00001268467"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 2 ENSMUSE00001268467"
     exon            15318..15461
                     /db_xref="ENSEMBL:ENSMUSE00001264263"
                     /type="targeted"
                     /rank="9"
                     /note="target exon 3 ENSMUSE00001264263"
     misc_feature    14500..15641
                     /note="Critical Region"
     misc_feature    15665..15698
                     /note="Downstream LoxP"
     misc_feature    15642..15721
                     /note="synthetic loxP region"
     exon            16749..16824
                     /db_xref="ENSEMBL:ENSMUSE00001260696"
                     /rank="10"
                     /note="ENSMUSE00001260696"
     exon            16908..17070
                     /db_xref="ENSEMBL:ENSMUSE00001257388"
                     /rank="11"
                     /note="ENSMUSE00001257388"
     exon            17241..17337
                     /db_xref="ENSEMBL:ENSMUSE00001309108"
                     /rank="12"
                     /note="ENSMUSE00001309108"
     exon            17418..17485
                     /db_xref="ENSEMBL:ENSMUSE00001290617"
                     /rank="13"
                     /note="ENSMUSE00001290617"
     exon            18495..18558
                     /db_xref="ENSEMBL:ENSMUSE00001254107"
                     /rank="14"
                     /note="ENSMUSE00001254107"
     exon            18690..18781
                     /db_xref="ENSEMBL:ENSMUSE00001299557"
                     /rank="15"
                     /note="ENSMUSE00001299557"
     exon            19083..19259
                     /db_xref="ENSEMBL:ENSMUSE00001302276"
                     /rank="16"
                     /note="ENSMUSE00001302276"
     exon            20578..20640
                     /db_xref="ENSEMBL:ENSMUSE00001298691"
                     /rank="17"
                     /note="ENSMUSE00001298691"
     exon            20754..20830
                     /db_xref="ENSEMBL:ENSMUSE00001258770"
                     /rank="18"
                     /note="ENSMUSE00001258770"
     misc_feature    15722..21402
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggaagcaag ccacctaact cttctttctt tgttgctcct gtataaaata
     4261 ggaatgagag ctaagcatgt gggtctgtgt cagagctctt gtttagcacg ctccaggctg
     4321 tggcttcttt ccccaggacc acaaaagagc gactactacc aaggagaagg gaatttagtg
     4381 tgttgttaga gtgtaaacct cagtacccag aaggcactgg gcgaacctgg tctgttttct
     4441 ctctcccttc tttgaaaggg agacaggagc tagacactgc agggttctga gacactctgg
     4501 ggctctgcct tttgtatctg ttccctcttt gctgtggagc agggtaggct aagacttctc
     4561 tgccctttgc agcctgggac tggccagaga gcccccaagg atgagaaaga gatggtccgc
     4621 cgagccatcc agaaggagct gaagatcaag gagggcatgg agaacatgcg gcgcgtggcc
     4681 acagaccgac gccacctggg tcatgtgcag caactgctta gggcctccaa ccgccgcctg
     4741 gagcagctgc atggcgagct tcgggagctg catgcccaag ttctgctgcc agcctcagcc
     4801 ggtgagcgag gagtctgaat aaacctggga acgggagact cacgaacaca atcctggcct
     4861 tggctttgac caccctatat catccacatg ttgtctatac ggaagggaag ggaaaaaggc
     4921 aagcgctgct tccccggggt ctgaactcta gccttgtgtt gcagagccag tgacctcgga
     4981 accccagccg cgggcggagc agtccagggc tcggctctcg gaggctttac ataggcagct
     5041 gcaggtagag ctaaaggtaa agcagggggc tgagaatatg attcacacgt gtgccagcgg
     5101 cacccccaag gtaagggctg gcgtctgatg aagaggggta ggcctggctt ctcatctacc
     5161 tgatgggaga gacgtagctc tcaggtataa ggatcacaaa gccagccaga ccctgtgcca
     5221 acagggaaat gctaccctgg cccagctcct cacctaatgc tagaagtaca ggtaggtggg
     5281 tgaggcaagt ctctgaccca cactgtcagc aaagcccaac aacagaaggc tagcggaaga
     5341 tccagccggc tgcccgtcca gctcttggca acctaccctc tcttctccat ctgacagaat
     5401 ccttactact tagcttgtca aactgatgag tgtcaggttc cgctcacccc gccagcctcc
     5461 accccccatt acctagttca acaagggagg cttgcctgta gtatcttcct tgtaggagct
     5521 tgaaccttgg tataacaggg gaggctcagc cccacccagg ccctggtggt ctgccgtaag
     5581 ccccttctga tggggcgagt gtcacctccc caggagagga agctcctggc ggctgcccag
     5641 cagatgttaa aggacagtca actgaaggtg gccctgctcc ggatgaagat aagcagcctg
     5701 gagtccagtg gctctccaga gccaggtgag gcctctcagg tcagggaggg tggccctcga
     5761 tggccgaggc taagggttca cctccgccct gcaggccccg acttgctggc agaggagctg
     5821 cagcaccgac ttcgagtaga ggctgctgtg gctgcaggtg ccaagaatgt ggtgaagctg
     5881 cttggtggcc agaggatgca ggaccgaaag gcactggccg aggtcaggct gtgtccccgg
     5941 ctcactgctg tctctacagc tctgtttggg tctatccaca tgcagtgggg cctgcaccca
     6001 gggttgctgc ctgtggaggg tagtagtgaa tacccatgag gtgtggcctg acccctttaa
     6061 cccatacctg gtgtttgtgt ctccttggta ggcccaggcc cagctccagg aatcctccca
     6121 gaagctggac ctcctacggc tggccctgga attgctgctg gagaggttgc ctcccaccca
     6181 ctctctacgt agcagggtga cccaagaact ttggatggca atgcttggga acccccaacc
     6241 tttggggaca cttgtgaaac ctattgcctt gacaggtagg tagcaaggat tccctttcac
     6301 gtcagagggt cccttacctt ttagagagga cgctgaagca tttgggagag agttcagtcc
     6361 tgctgtgtga ccttggatcc ctcatgttcc cactctgatt cttggtttat ccatctgtaa
     6421 catagattca atagtagtga cccagcatga tggcgcacgc ctttaatccc agcactcggg
     6481 aggcagaggc agggggatgt ctgtgagttc caacctggtc tttagagtga gttcgaggcc
     6541 agccagagag acatgggaga gaccctaagt aaacaaaggg tggaagtgct ctcctgatcc
     6601 acaagatagt tttgtaaacc caaaagtttc tgatgttcag aacacacttg gagcctggcc
     6661 agcgtggatg ggatttaaat gcccggcctt cggtagggag tagattgaca atcagtccac
     6721 tccctggcag ccttggggcc tgggcagctt ggactacctg taagttcttt catgaggtct
     6781 gttcttgtag cagcagctga ctggcagtca acagatcatg ggagctgcaa gctagagccc
     6841 ggctggtgcc tgcttcagaa cacaatagat agggctagac agagctctgt tactattatc
     6901 ttggataatc aaggagggct ccttagaaca ggggggagtc tgagcttggc cttggagggc
     6961 tctcgggagt gctgatggcc aagccagcaa gtccggcaag acaaaaggag cctcttgttt
     7021 aaaggcaaag aattggggac acgttgataa agttctttct tactgcgtat gaggtcctgg
     7081 gttcagtccc agcaccaaat taactgggta tggtagcaca cacctacaac ccaaccccct
     7141 gggaagtgag gcaggaggat caggagttca agggcatctt tagctacata gtgagtttga
     7201 ggccaacttg ggctaacaag atcctgcttt taaacaaaaa catttgctag tttgtagccg
     7261 aacttccatt tcctaagaca agtgtattgc ctgggccgcc cttggtattt ctgtaaggtc
     7321 tccaagtatt acctccagac ccagcctagt aaaatggtgt cctcagagcc ggtttataag
     7381 gcgcataacg ataccacgat atcaacaagt ttgtacaaaa aagcaggctg gcgccggaac
     7441 cgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcg aaccctttcc
     7501 cacaccaccc tccacacttg ccccaaacac tgccaactat gtaggaggaa ggggttggga
     7561 ctaacagaag aacccgttgt ggggaagctg ttgggagggt cactttatgt tcttgcccaa
     7621 ggtcagttgg gtggcctgct tctgatgagg tggtcccaag gtctggggta gaaggtgaga
     7681 gggacaggcc accaaggtca gccccccccc cctatcccat aggagccagg tccctctcct
     7741 ggacaggaag actgaagggg agatgccaga gactcagtga agcctggggt accctattgg
     7801 agtccttcaa ggaaacaaac ttggcctcac caggcctcag ccttggctcc tcctgggaac
     7861 tctactgccc ttgggatccc cttgtagttg tgggttacat aggaaggggg acgggattcc
     7921 ccttgactgg ctagcctact cttttcttca gtcttctcca tctcctctca cctgtctctc
     7981 gaccctttcc ctaggataga cttggaaaaa gataagggga gaaaacaaat gcaaacgagg
     8041 ccagaaagat tttggctggg cattccttcc gctagctttt attgggatcc cctagtttgt
     8101 gataggcctt ttagctacat ctgccaatcc atctcatttt cacacacaca cacaccactt
     8161 tccttctggt cagtgggcac atgtccagcc tcaagtttat atcaccaccc ccaatgccca
     8221 acacttgtat ggccttgggc gggtcatccc cccccccacc cccagtatct gcaacctcaa
     8281 gctagcttgg gtgcgttggt tgtggataag tagctagact ccagcaacca gtaacctctg
     8341 ccctttctcc tccatgacaa ccaggtccca ggtcccgaaa accaaagaag aagaacccta
     8401 acaaagagga caagcggcct cgcacagcct tcactgctga gcagctccag aggctcaagg
     8461 ctgagtttca gaccaacagg tacctgacag agcagcggcg ccagagtctg gcacaggagc
     8521 tcggtacccg gaagatctgg actctagaga attccgcccc tctccctccc ccccccctaa
     8581 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     8641 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     8701 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     8761 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     8821 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     8881 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     8941 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     9001 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     9061 gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     9121 cgatgataag cttgccacaa ccatggaaga tcccgtcgtt ttacaacgtc gtgactggga
     9181 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     9241 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     9301 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
     9361 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
     9421 gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga
     9481 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
     9541 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
     9601 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt
     9661 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct
     9721 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa
     9781 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg
     9841 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
     9901 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
     9961 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
    10021 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
    10081 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
    10141 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
    10201 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct
    10261 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct
    10321 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac
    10381 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag
    10441 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc
    10501 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc
    10561 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga
    10621 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa
    10681 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga
    10741 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg
    10801 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa
    10861 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa
    10921 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct
    10981 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga
    11041 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc
    11101 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa
    11161 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac
    11221 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg
    11281 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat
    11341 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca
    11401 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct
    11461 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga
    11521 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca
    11581 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac
    11641 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg
    11701 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc
    11761 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg
    11821 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg
    11881 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct
    11941 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt
    12001 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca
    12061 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga
    12121 ctcctggagc ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca
    12181 gttggtctgg tgtcaaaaat aataataacc gggcaggggg gatctaagct ctagataagt
    12241 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
    12301 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
    12361 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    12421 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    12481 gatccggaat aacttcgtat agcatacatt atacgaagtt atgtttaaac ggcgcgcccc
    12541 ggaattcgcc ttctgcagga gcgtacagaa cccagggccc tggcacccgt gcagaccctg
    12601 gcccacccca cctgggcgct cagtgcccaa gagatgtcca cacctaggat gtcccgcggt
    12661 gggtgggggg cccgagagac gggcaggccg ggggcaggcc tggccatgcg gggccgaacc
    12721 gggcactgcc cagcgtgggc gcgggggcca cggcgcgcgc ccccagcccc cgggcccagc
    12781 accccaaggc ggccaacgcc aaaactctcc ctcctcctct tcctcaatct cgctctcgct
    12841 cttttttttt ttcgcaaaag gaggggagag ggggtaaaaa aatgctgcac tgtgcggcga
    12901 agccggtgag tgagcggcgc ggggccaatc agcgtgcgcc gttccgaaag ttgcctttta
    12961 tggctcgagc ggccgcggcg gcgccctata aaacccagcg gcgcgacgcg ccaccaccgc
    13021 cgagaccgcg tccgccccgc gagcacagag cctcgccttt gccgatcctc tagagtcgag
    13081 atccgccgcc accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
    13141 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
    13201 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
    13261 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
    13321 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
    13381 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
    13441 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
    13501 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
    13561 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
    13621 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
    13681 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
    13741 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
    13801 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
    13861 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
    13921 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
    13981 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    14041 ctggagttct tcgcccaccc cccggatcta agctctagat aagtaatgat cataatcagc
    14101 catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    14161 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    14221 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    14281 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ggggtaccgc
    14341 gtcgagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcgtcgaga
    14401 taacttcgta tagcatacat tatacgaagt tatgtcgaga tatctagacc cagctttctt
    14461 gtacaaagtg gttgatatct ctatagtcgc agtaggcgga aacatgtttt gtccccattg
    14521 ggggctctcc ctcgctgatc aggtgggaag gaatagggca ggcagctaga gtgatgctcg
    14581 ggtgcctgcc cctgtgcact tgagaccatg gtgcccacca gaccctgagc tcaaggtagc
    14641 agtttccctc atcctgtggc tttacaaagc ttgggagaag tcctgttctt acattcctgc
    14701 tgtgttctgc cactgcatag gcactcagga aggagagcta gccgggctgc taaggatgct
    14761 gggtggtaac taagtgtggt aggtagggtt tagatcggct gctcctagct ctgaccctcc
    14821 tagagcaggt ggtgtccctc tggggttcca ttggtggtgg ctgctgacag agggctctct
    14881 tgactcccaa ccagggacac tgcaggttcg cctcctgggc tgcaaagatc tgctggtagc
    14941 tgtgcctgga cggtctccca tggctgtcct ggcaggtagc ccttctgaaa gctggctccg
    15001 aacaaggtcc cggcagcagc gtggtggagg cgaactggcc agtgagtagg gagaaaccat
    15061 gggaggtggt gaggccccct gctctggcca tgccctaaat ccaagccctg gcactacagg
    15121 tgaggtactg gctgtgttga aggttgacaa tcgtgttgtg ggccagacag gatggggact
    15181 ggtggctgag aaatcctggg accagagttt tatcatctcc ttggaccgag taggctggcc
    15241 tttgtgtggt tagggttagc tgtggggtac gggggggggc actcaggtga gggctgagcc
    15301 tgatcttttc cacataggct cgcgagctgg agattggggt tcactggcgg gactggcgac
    15361 agttgtgtgg tgtggcgttt ctgaagcttg aggacttcct ggacaatgcc tgtcaccagc
    15421 tttctctcag tctggtgcct caggggcggc tctttgccca ggttcctact gacccctgac
    15481 ctctaacagg ggatctttag gactgacatt ttgggttggg gtgatggctc agtgggtaaa
    15541 gtgtttactg tggaagcatg aggacctgag ttcaaatccc caggatctgt gtaaaaacca
    15601 tgtgtggctt ggtgtgtctc taagttcatg gctggccgta cgagatggcg caacgcaatt
    15661 aatgataact tcgtatagca tacattatac gaagttatgg tctgagctcg ccatcagttc
    15721 acagataccc agtgtggtgt caacctctgc ctacatcagt gagtacaact ctgtgtgtgt
    15781 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgcat ctgtgtgata aaaactggta
    15841 atccatgtac tatgggactt gagagatgga gacaggttca cagacaggcc tacagggagg
    15901 ctcacatctc tacactgagc ttgtcctgcc tcatatgtca tcttgaatac gtaaatgtgt
    15961 ctcctctggg cctcagtttc tcccctggcc cccgtgagga tacggtatcg tgtctaggtc
    16021 ctctgagttt tgcttcatat cagctttgcc tcattgttcc gataaaacac cggccaaagc
    16081 aactgaaggc agggaagggg tgatttcaat ttacagattt acagccaacc ccctccctac
    16141 tcttctccca ccccccccca ccccccaccg ccttctttat tttttctgag atagggtttc
    16201 tcttgcagcc ctggctgtcc tggaactcac tctgtagacc aggctggcct ccaattcacg
    16261 gagatccacc tgcttctgcc tcctcagagc tgggattaat ggtgtgctcc accaccgccc
    16321 agtttacagc ctatctttac gggagccaag gcagaaactt aataagcagg aactgaaagc
    16381 agaaactggg gaggaaccgt ttgctggctt gctctctgcc tctctcccaa gctcatgctt
    16441 ggctagcttt cttatatagc ccagtaccac ttgtctaggg aatggtgcct ctaacagtgt
    16501 gctagatcct cctacatcaa ttaacaatca agaccccccc ccagacatgc ccacgagcca
    16561 tgtaatctag gcagtccctc tcggatgggt catatggaaa gttagcacta actagaatac
    16621 ccccatggct gagattggga gctggcgctg ggggtggggg agtggggatg ggggtgggga
    16681 cggggacgat ccagcagggt gaaaggcggc cagtctccag gtcttcagct ttcttcatgt
    16741 ccctttaggt caccttctgc gagcctgtca ttgaaaggag gcctcggctg cagaggcaga
    16801 gatgcatttt ctctaagcgg agaggtgtgg agcatacgga ctgtgtgagg agagggctgg
    16861 gctgaccaat ggggatgctg ggactgagcc ccgcctgcct cctctaggcc gggatttcat
    16921 gagagcttcc cagatgaacc tcagcatggc agcctggggg cgtctggtca tgagcttgct
    16981 gcccccctgc agctcaccaa acacagccag tccccctaaa gggcgccctt caacagcggt
    17041 ctgtgggacc cccagtgctg cttccccgag gtgagccgcc tggtgcagac tctgagacac
    17101 ttttcaggct ctgtctcttc aggttctgag tgctgtcttt tgcctcctca acctctgagc
    17161 cctctgtcct ttcacatggc cgcgcccctc accccccccc ccccctaatg tgtctgggct
    17221 gagacatctc cttcttacag taacttcctg cccatgaaga cgctctcaaa agaagacacg
    17281 aagcctcctc ccaagccccc gcgcctctat ctccaagaac cagccccagg gactcctgta
    17341 agggattctg gaggtcaggg ctgccaggag ggaaagaggg tcagtaactg ctgtcctgaa
    17401 gccatctgtc cccacagtgt accaagcgcc cccacatgga ccctagacct gcagtagtgc
    17461 ccgccctggc agccttatcc acaaggtact cactggcttc cattcattta gagacttttg
    17521 tcttgttttg ttttgttttt taaaaatcta aatatatatt tatgtgtgtg tgccttcgtg
    17581 tgcacacaga cccatgctga agcacaattt tatagtgact ccattttatt tcacgttcct
    17641 tggtgttttg cctgcatgtg tgtctgtatg agtgagtcag gtgctctgga agttgtgagc
    17701 cactgtgtga atgctgggag ttgaacctgg ggtcctctgg aagagcagcc agtgctcttt
    17761 ttttttttta aagatttatt tatttattat atgtaagtac actgtagctg tcttcagaca
    17821 tcccagaaga gagcttcaga tctcattaca gatggttgtg agccaccatg tggttgctag
    17881 gatttgaact cacgactttc agaagagcag ttggcactgt taaccgctga gccatctctc
    17941 cagcccccag ccagtgctct taaccctgag ccatctcttc tttgtttttt tatttcctcc
    18001 ttcctcctcc tgctcctcct cctcctcctc ttcctcctcc tcattctctt tctcctcctc
    18061 ctttctctgt gtagctctct cgctgcccag gaactcccta tgtagaccag gttggcctcg
    18121 aactcagaaa tccgcctacc tctgcctccc aagtgctggg atcaaagacg tgtgccacca
    18181 cggccctgct tgcatccaac ttttctaaag ttggtctggg catggaacgc aagccatttc
    18241 cctgcctgag actcttttta aaaattaagg tcttacagtg taacccaggc tggttgaact
    18301 tatgattttc ctgcttctgc agcctgggtg ctgggattac aagtgtgcac catcgctcct
    18361 tgtatatatt gattgacacc tcactccatt gctcctccat tatacaaact gccttgctca
    18421 atgtcttttt gtgctgaatg cagctttaaa attctagatg tggggagaca gcctaggccc
    18481 ttcctctctt gcaggaaacc ccccagactt caagacttcc gatgtttggc tgtgctgggt
    18541 cggggacact ttgggaaggt aggtagttga gtgggtgggc aagcattgtc tcagcacatt
    18601 agctggagat gctgggatgg agggaatgtg tggggagatg aaggctctct gtccttcgta
    18661 cctggagtcc aactttttct ggtccctagg tcctcctggt ccagtacaaa ggaacaggga
    18721 aatactacgc tatcaaggcg ttgaagaagc aggaagtgct aggccgggat gagattgaca
    18781 ggtgtatata tttgagaagt atctgatctt ggatctgggg tggccaacct gggctcccaa
    18841 ggtagctcga gatcccaagg gtccgtttca ggttgtcttt ttggccctgg gcaagccctc
    18901 ttgtctttta gtaccccggg gaactcgtct ataaatagga ctgtggtact ttggcaggtt
    18961 tgaagccttc cccatgtgtg aaccatcaac ttgcctcctt agctctcctc agccagccag
    19021 aggtgttgta tgggtgtcag ggcacagctc atgggggttc cattccatgt cctcccttgc
    19081 agcctatact gtgagaagcg gatcctggag actgtggggc gtacagggca ccccttcctg
    19141 ctctctctcc ttgcctgtct gcagacctcc agccatgcct gctttgttac tgagtttctg
    19201 cccggaggag acctcatggc gcagattcat gaggatgtct ttcctgagcc ccaggcctgg
    19261 tgggttctct ggctacttgg tgcttgctcc ttgggggtag gggagtaccc agcagacaaa
    19321 tagcatggga ttgtgaggag tcttggtggg acattacaac acatgaggac ttcatttgca
    19381 tagccgtgga tgaaatctgt catcatgact ttgcaagttt gttgcaggga tgagagtcat
    19441 gtttgctact tgcctaccgc aggatagaag cagggtctgc ctacccagct ccagttgctg
    19501 ctctgcttac aacagtctga ctccgcacat cagccctcac cctagtgtgt gaccctgagt
    19561 caaatctcag atcctcattt cactgcgtgt atgtgtggct gttcatggcc acctgcaatt
    19621 gagtacagag ccctcagcca ccagaagagg gcacacatca cctgaagcag gagtggctgg
    19681 atatctacct gatagaggaa ctcttgtcta ggagtgggta gtggggcaga gaatagtact
    19741 tcctattaaa tgccaggcca tctctccagc ctctcagatc cagacttgag tcaaagtcat
    19801 gatgactatc ccccgaggct gctgggaaga tgtccatact gagctttgat cctagggctt
    19861 agtgtgagcc agactctatc actgagctac atctccagcc ctttatggtt ccttttctgt
    19921 ttatttgtgt tatgtttttt agttgttgtt gttgtttttt tttttagatt tatttattta
    19981 ttacatgtaa gtacactgta gctgtcttca gaccctccag aagagggagt cagatctcat
    20041 tacggatggt tgtgagccac catgtggttg ctgggatttg aacttcggac cttcggaaga
    20101 acagtcgggt gctcttaccc actgagccat ctcaccagcc cgtgttatgg ttttttgaga
    20161 gggtttctct gtatagtcct ggcttttctg gaacttgctc tataaaccag gctggccttg
    20221 aactcagaga tctgcctgct tttgcctccc gagtgctggg attaaaggcg tgtgccgccg
    20281 ccaccgccac caggcttttg tttgcttttt gaaaactata tagaccagtc tggcctcaaa
    20341 tttgctatcc tcctgcctca gcctcccaag tagctgggat tataggccta tactactaga
    20401 cccagtagct ctgccatagc tgagcacagg aaggataggt cattaataca tagcaagact
    20461 ggaatatttt gggcttctcc agtattagtt tgtttagtcc catccatgcc tgagtctggc
    20521 caggaccttt tggtaccttt cagtgccagg cctgaactct ccctgtacct ccctcagctt
    20581 ctacctggcc tgtgtggttc tggggctgca gttcctacac gagaagagga tcatttacag
    20641 gtagtctttg cccaagagat gctgggtggg cctgggagct ttccttctgc tgatgctggg
    20701 ccccaggcct cagcccctct caaccccttt gatcttcatc actccaccat cagggatctg
    20761 aaattggata atctgctact ggatgcccag ggtttcctca agattgcaga ctttggacta
    20821 tgcaaggaag gtggggcccc ctgtcttgta atcacatcct cttgtcttgc ctagaccagc
    20881 cagaaatctc tccactcatg gtgtccagcc ctggccttca ttcctgaccc ttgtttaggg
    20941 gaacagctga gaatacccta atttgtagtt ggccagagaa gctatgatga ccttggcaca
    21001 caagttcatc agaaacaact ctcaaacagt cactggctgc acaatcacct tggcctagag
    21061 agtctagggg atacagtaaa ccttgttatt tggtgtcata aagacgggca ccaagctggg
    21121 tggtggtggt gcacgccttt aatcccagca ctcaggaggc agaggcaggc ggatttctga
    21181 gttcaaggcc agcctggtct acagagtgag ttccaggaca gccagggcta tacagagaaa
    21241 ccctgtcatg aaaaaaaaaa gtgtgtgtat gtatggtata tatcacatac acacatatat
    21301 gtatatatat atatatatat atatatatat gcatatgtat atgtatgtat atatgtattt
    21361 tattgctggg ttttgaccca tggtctcaca tgtactaggc aa
//