LOCUS       KO-first_condition_ready_30600_MGI:2388270        20663 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Acap1 targeting project(s): 30600
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_gt1
COMMENT     design_id: 40074
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     exon            8849..9018
                     /db_xref="ENSEMBL:ENSMUSE00000111702"
                     /rank="17"
                     /note="ENSMUSE00000111702"
     exon            8660..8754
                     /db_xref="ENSEMBL:ENSMUSE00000111703"
                     /rank="16"
                     /note="ENSMUSE00000111703"
     exon            8475..8544
                     /db_xref="ENSEMBL:ENSMUSE00000326974"
                     /rank="15"
                     /note="ENSMUSE00000326974"
     exon            7736..7947
                     /db_xref="ENSEMBL:ENSMUSE00000326882"
                     /rank="14"
                     /note="ENSMUSE00000326882"
     exon            7524..7647
                     /db_xref="ENSEMBL:ENSMUSE00001306639"
                     /rank="13"
                     /note="ENSMUSE00001306639"
     exon            6272..6372
                     /db_xref="ENSEMBL:ENSMUSE00001301959"
                     /rank="12"
                     /note="ENSMUSE00001301959"
     exon            6133..6184
                     /db_xref="ENSEMBL:ENSMUSE00000578702"
                     /rank="11"
                     /note="ENSMUSE00000578702"
     misc_feature    4112..9144
                     /note="5 arm"
     misc_feature    9172..9196
                     /note="B1 Gateway"
     misc_feature    9209..9256
                     /note="Frt"
     intron          9257..10131
                     /note="mouse En2 intron"
     misc_feature    10129..10131
                     /note="Splice Acceptor"
     exon            10132..10170
                     /note="mouse En2 exon"
     misc_feature    10171..10187
                     /note="Frame 1 insert"
     misc_feature    10188..10241
                     /note="'self-cleaving' 2A peptide"
     gene            10242..13319
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13320..13373
                     /note="'self-cleaving' 2A peptide"
     gene            13374..14165
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    14364..14606
                     /note="SV40 polyadenylation site"
     misc_feature    14623..14670
                     /note="Frt"
     misc_feature    14677..14710
                     /note="loxP"
     misc_feature    10146..10261
                     /note="FCHK std_gt1"
     misc_feature    10146..10163
                     /note="FCHK QC prefix region"
     misc_feature    10164..10243
                     /note="FCHK QC critical region"
     misc_feature    10244..10261
                     /note="FCHK QC suffix region"
     misc_feature    14726..14746
                     /note="B2 Gateway"
     misc_feature    9145..14776
                     /note="Synthetic Cassette"
     exon            15140..15312
                     /db_xref="ENSEMBL:ENSMUSE00000111713"
                     /type="targeted"
                     /rank="18"
                     /note="target exon 1 ENSMUSE00000111713"
     misc_feature    14777..15473
                     /note="Critical Region"
     misc_feature    15497..15530
                     /note="Downstream LoxP"
     misc_feature    15474..15553
                     /note="synthetic loxP region"
     exon            17182..17340
                     /db_xref="ENSEMBL:ENSMUSE00000409783"
                     /rank="22"
                     /note="ENSMUSE00000409783"
     exon            16876..16925
                     /db_xref="ENSEMBL:ENSMUSE00000111701"
                     /rank="21"
                     /note="ENSMUSE00000111701"
     exon            16415..16525
                     /db_xref="ENSEMBL:ENSMUSE00000111712"
                     /rank="20"
                     /note="ENSMUSE00000111712"
     exon            16213..16322
                     /db_xref="ENSEMBL:ENSMUSE00000111704"
                     /rank="19"
                     /note="ENSMUSE00000111704"
     misc_feature    15554..20663
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg aacaactgtc tcagctaaac ccaaccagtt
     4141 gaatattctc ggatcaataa tgattgtgta ttgtatatga agtacatcaa gatacaaccc
     4201 ttagtcagca aagacagaca aacagatcag taactaaata actgcatact atgttagaag
     4261 gtaataataa aacatctggc acaatggcac gctcctttaa tcccagctca ggggaggcag
     4321 aaataggcat atctctatga gttcaaggcc agcctgatct acaacatagc aagttccagg
     4381 actgccaggg atacatagag gaaccctatc aaaataataa taataataat aataataata
     4441 ataataataa taataataat aataataagc tgagcatggt ggcgcacacc tttaatccca
     4501 gcacttggga ggcagaggca ggcagatttc tgagttcgag gccagcctgg tctacaaagt
     4561 gagttccagg acagccaggg ctacacagag aaaccctgtc tgaaaaaaca acaacaacaa
     4621 aaaaaaccaa taaccacaac aacaagggat ctacagctga gctacaccca caatgtaggt
     4681 tcccaattct aaataaacta tcaggggctg ggtttggtgg atcaggcctg caagcctagc
     4741 actggcaagg aacagagagg tttggaactc tctctctctc tctctctctc tctctctctc
     4801 tctctctctc tctctctctc ttttttggtt ctttgagaca gggtttctct gtgtagccct
     4861 ggctgtcctg gaactcactc tgtggaccag gctggcctcg gaactcagaa atccacttgc
     4921 ctctgcctcc caagtgctgg gatcaaaagc ttgcaccacc actgcctggc taggattgga
     4981 attctgtctg caaacaagta aggaacaaaa ctgttacttt atttcttgac acactctatg
     5041 ggaaaatctc ttggctgatg tagccaagga tgaccttgaa ctccggatct ttgggcctcc
     5101 acctcccaag agctgggatt acaagtgttc accatcatgc cgggctaaga aagtaacttc
     5161 taagtcaaga tatctgagtg aaggatgctg taggcagaag gacaagttaa tgggaaggaa
     5221 ggccctgagt tcccagtatt tgcaaaatga gttgtgggcc gggacagagt gagggaggaa
     5281 ggaagaggag ctaaaggggc tacggaatcc ttctagaact ttgcagatgt tggtaggact
     5341 ctgagactac tctgaggaag atggggacac cctacagggc ttgaaacaga atgctacgtg
     5401 tcttgatgtg actggttcat ctttcagaac cacccccagc ccataaggga ccttggtaca
     5461 aattagaaaa tggagcccct tccctaacat agtttcattt gcccaacact gtagcaataa
     5521 gcttggtgta aatgccagca gtcaggcgac taaggggaga ggattgcaga tttgtggtca
     5581 gcctggacta cagagcgact tttaggctta cccgggttac atggcacgat cctatctcaa
     5641 acaaatactc gtagaagtga ttccaatacc ctcaggagcc acccgaaaca gtgtctatca
     5701 ctgttaccca tttttataac ctttaaggct gtctccccct gctagctcct tcaacattgg
     5761 ccaagcttga caaaaaggta caataataaa cttagaagaa agatatgtgg tggcatatcc
     5821 ttgtgagcct ggcttttgag gtggaggtag gaagatcagg agttgaaggc cagccttact
     5881 gccatctgct ggaaggtatg tccaatctcg gttgtccaag gtgtgcagaa cagcccttaa
     5941 ggagctgtaa gggtgtaccc attctgcgtg taagttccca ccattgtctg gaatgtccca
     6001 tgggactgtc agtccacacc tctctcctca gtgagctact tagggggcaa agagcacagt
     6061 ttctcttctg tccactcctg agcctgtccc catgttgggc atgcatgtaa ccacttcttc
     6121 tgtctcctcc agacgctggt tcaccattca gaacaaccag ctggtttacc agaagaaata
     6181 taaggtgggt tggtctgggg tgcctggact ctagataaac tcagcctttg ttcacaatga
     6241 ctggctcatg gaccttatca ctctctgcca ggaccctgta accgtggtgg tagacgacct
     6301 tcgtctctgt acagtgaagc tctgtccaga ctcagaaagg cgtttctgtt tcgaagtagt
     6361 gtccaccagc aagtgagtag agtccctggg ttgatattct aaaatgtctg aatgtttaca
     6421 aacccaaaag gcagggccac caaaacagta tccctgaata ggtccaggac ttaacccttt
     6481 ggtgactggg ttgaagcaga aagcccagac tgagaggaca agccggtagg agatatttcg
     6541 gaggacgctg ccattcacta gaggagtacc agtgctccgc ttacagtaaa ttcattgtca
     6601 tttgaaaaga tactggaaaa cctactttac acatagctta gcctagcctt agcttacctg
     6661 ctagaaaccg ttagcataca attccaaggt tcttttacat gaaagtgtta ttgaacatgc
     6721 tactgaaaac ccaagagggt tgtataaata cacctttgtg tgttaatctt gaaaaatatt
     6781 gtcaaactac cataagttag ggatcacctg gatttcagta gagcagataa ccaggtacgg
     6841 agcacacctg taacgccagc acttgggagg agaaccttaa gtttgaggaa catggcctgt
     6901 ttctgactca aggagaaaga ggctactgag atggctcagt acttgtgcca agctcgtcga
     6961 cctgagttca tccccaggac gcacatggtg gaaagagaga ctcaactcca cgacatactg
     7021 ggtgtgtgtt tgcacagaca cacagacaca cagacacaca catgtgaata taatgtgtct
     7081 ctctccaccc caagaagggg gttctctgtg tagccctgac tgtcttggaa ctcattctgt
     7141 agatcaggct ggcctccaac tcagagatcc acctgcctct gcttcccaag tggtaggatc
     7201 aaaggtgtgc accaccacca ccgggttaat gttgtctttc tgaaaaattc aaggcccgaa
     7261 tggcttgcta gcaagtttga agtcagtctg gaaagtttat cagatcctat ctctaactaa
     7321 agtaaaaaat gtcttggata taactccgag gcagcgtgct tacctaagca tgcgcaaagc
     7381 cttgggccca atctcattag aaatagatca ataagtaaac atctttcaaa atctttcaaa
     7441 atcagctggg catccacaga ctgtcagctt gccctccctg cctcacggct ctctggtctt
     7501 gtcactctca atatcacctg caggtcctgt ttcctccagg ctgactctga gcgtctcctg
     7561 cagctgtggg tcagtgctgt ccagagcagc attgcctcag ccttcagcca ggctcacctg
     7621 gagaacagcc ctcgggggcc aggccaggta ccttcatatg ggccacactg ccctgggagg
     7681 tggagctttc ctcaccctta ggccccttgc tcatcctttc tcctgtctcc ctcaggtctc
     7741 aggataccat gccccgggct ctgctgcaac tctggcctgt ggcggggcag ccagaggaag
     7801 ggagtctgga ggagttggtc aggtggcggc ccaggtacaa agcgtggatg gaaatgcgca
     7861 atgctgcgac tgcagggagc cagccccaga gtgggccagc atcaaccttg gcgtcactct
     7921 ctgcattcag tgttctggca tccacaggtc attccctaag acccgaggca caagccccgc
     7981 ttccaactgc tgcgtggccc ctctcatgca ccctaccctt gctcttcctt ctctgtcacc
     8041 agtctccaag gttgtagcgc aagtgcacat ttgagttgga tgtggacagg cctgggggcc
     8101 tcctagatga actcattgtc ttgataggaa gaagcatggg ttacggttgc tgagggtggg
     8161 acagccaaca agagcccaag agggaaaggg ttgtgagatc ctagaaagcc cctaaggctt
     8221 caccaccagt ctgaagtgta tggagggaaa agaggtggca gacaaaacat gttccagacc
     8281 gaagcatcac gagcaaagtg gggaggctag agtgttcagc agcaatggtg agcaggccag
     8341 agctagaggg cctctgctgc tctgcttcct ggcccggctc ctctgtgtct gcacacaggc
     8401 ctggtctttt caggagcaac agctttccta ggctactgcc cacacagggc atgactctcc
     8461 ctctggccct ccaggagcct cggagttcat ttctccaaag tccgctctct gacccttgac
     8521 tcctgggaac cagaactggt gaaggtaact agggttactc gggatattgt gaagacagag
     8581 gactgaggag tctttgaggg ctctgatact cacccagtga ctctccctct ttctcttgcc
     8641 ctcttctggg ccttcgaagc ttatgtgcga gctggggaat gtcatcatca accagatcta
     8701 tgaggcccgg gtggaggcca tggcggtgaa gaagccaggg cccagctgct cccggtgagg
     8761 ttgcggtgga ggcacctagg gcttccctgt cgtggagagg aatggcaaag aggccgaggg
     8821 accaaaggct gtctcctcta acctccaggc aggaaaagga agcctggatt catgccaagt
     8881 atgtggagaa aaagttcctg accaaacttc ctgaaatccg tggacgaaga ggtggcaggg
     8941 gacccccaag aggacatcct cctgtgcccc caaagcctcc catcaggcca cactcgggca
     9001 ttgtcagatc caaatcaggt agagagtcca actcattgct gtacactcag tactggccag
     9061 gccctagagg gaggcagaat gtgaggtcca ggctgtccct tggaggaggt tgggaggagc
     9121 agagtacagt caggaggtca cagtaaggcg cataacgata ccacgatatc aacaagtttg
     9181 tacaaaaaag caggctggcg ccggaaccga agttcctatt ccgaagttcc tattctctag
     9241 aaagtatagg aacttcgaac cctttcccac accaccctcc acacttgccc caaacactgc
     9301 caactatgta ggaggaaggg gttgggacta acagaagaac ccgttgtggg gaagctgttg
     9361 ggagggtcac tttatgttct tgcccaaggt cagttgggtg gcctgcttct gatgaggtgg
     9421 tcccaaggtc tggggtagaa ggtgagaggg acaggccacc aaggtcagcc ccccccccct
     9481 atcccatagg agccaggtcc ctctcctgga caggaagact gaaggggaga tgccagagac
     9541 tcagtgaagc ctggggtacc ctattggagt ccttcaagga aacaaacttg gcctcaccag
     9601 gcctcagcct tggctcctcc tgggaactct actgcccttg ggatcccctt gtagttgtgg
     9661 gttacatagg aagggggacg ggattcccct tgactggcta gcctactctt ttcttcagtc
     9721 ttctccatct cctctcacct gtctctcgac cctttcccta ggatagactt ggaaaaagat
     9781 aaggggagaa aacaaatgca aacgaggcca gaaagatttt ggctgggcat tccttccgct
     9841 agcttttatt gggatcccct agtttgtgat aggcctttta gctacatctg ccaatccatc
     9901 tcattttcac acacacacac accactttcc ttctggtcag tgggcacatg tccagcctca
     9961 agtttatatc accaccccca atgcccaaca cttgtatggc cttgggcggg tcatcccccc
    10021 ccccaccccc agtatctgca acctcaagct agcttgggtg cgttggttgt ggataagtag
    10081 ctagactcca gcaaccagta acctctgccc tttctcctcc atgacaacca ggtcccaggt
    10141 cccgaaaacc aaagaagaag aacgcagatc gcagatcgca gatctccgag ggcagaggaa
    10201 gtcttctaac atgcggtgac gtggaggaga atcccggccc tgggatctgg actctagagg
    10261 atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    10321 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    10381 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag
    10441 aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc
    10501 cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca
    10561 ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat
    10621 ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta
    10681 actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt
    10741 tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga
    10801 tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg
    10861 gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg
    10921 ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt
    10981 gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg
    11041 tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg
    11101 atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga
    11161 atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag
    11221 cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca
    11281 agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca
    11341 tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg
    11401 tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc
    11461 tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga
    11521 ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg
    11581 atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta
    11641 atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg
    11701 aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg
    11761 atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac
    11821 ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg
    11881 gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg
    11941 tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg
    12001 cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg
    12061 tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt
    12121 tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata
    12181 gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg
    12241 aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc
    12301 agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg
    12361 catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca
    12421 gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt
    12481 tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac
    12541 agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg
    12601 caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg
    12661 tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg
    12721 cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga
    12781 aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta
    12841 ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc
    12901 agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc
    12961 ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata
    13021 ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg
    13081 gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac
    13141 tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg
    13201 acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat
    13261 tccagctgag cgccggtcgc taccattacc agttggtctg gtgtcagggg atcccccggg
    13321 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctattgaac
    13381 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
    13441 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
    13501 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
    13561 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
    13621 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
    13681 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
    13741 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
    13801 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
    13861 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
    13921 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
    13981 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
    14041 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
    14101 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
    14161 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
    14221 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    14281 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccacccccc
    14341 ggatctaagc tctagataag taatgatcat aatcagccat atcacatctg tagaggtttt
    14401 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    14461 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    14521 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    14581 caatgtatct tatcatgtct ggatccgggg gtaccgcgtc gagaagttcc tattccgaag
    14641 ttcctattct ctagaaagta taggaacttc gtcgagataa cttcgtatag catacattat
    14701 acgaagttat gtcgagatat ctagacccag ctttcttgta caaagtggtt gatatctcta
    14761 tagtcgcagt aggcggagag aggttgaact tggcatctcc aaaggtttga aaagaaggaa
    14821 gggttatgag ccttgtctgg gcgggggaag gtaagcactg aatctgtcag ggagtcagct
    14881 cacctctggc atccagccag tctggggaga tgagcaatgc tatctcttcc acacccaggg
    14941 ccctgcacac acacaacaca cataccaaca cgtatacaca tccacagcgc tcccgtttct
    15001 caccacagct ctgagtcaca agggactccc cacaatttgg acgtgactta tcccactgca
    15061 gcacctgggg actttgctga agattttctt tctagacccg gcactgatac tccttaacag
    15121 aattaaatcc acttgacaga gtgcccgtct gatgacatgg gaagcctgca ccctggggcc
    15181 ctgctgtttc aagctgctgg acatccccca tctcttccta ccatggctga tgccctggcc
    15241 catggagctg atgtcaactg ggtcaatgtg ggccagggga atgccacacc tctgatccgg
    15301 gccacagctg ctgtgaggac catgctgacc tccccaactc taccctagag ctctccctgg
    15361 ggtctccttc ccatagggca agataaataa tatagactaa gtgtttgaag tgtgatttgg
    15421 aggtggtgaa ttagattttt cgtctgctac ttacatgagc ttttaaacaa gttgagatgg
    15481 cgcaacgcaa ttaatgataa cttcgtatag catacattat acgaagttat ggtctgagct
    15541 cgccatcagt tcattaggag ctcatgctgc gtttgctcag gatcagttcc cagcatctac
    15601 aaggcagcac ataactgtaa ctccagtgcc aggagatcca gcgccctcta ctggccccct
    15661 cattcactgc atgtgacaca cagacataca tgtaggtaaa acacctatac acataaaata
    15721 aaaataaaac aatctttaat taaaaaaaaa aagagggaca gcatgactca tcagggtgca
    15781 ggatatgctt tggggaggga cagattttct ctgtgtagcc ctggctgtcc tggaactcac
    15841 tctgtagacc aggctggcct taaactcaga gatttgctgg cctctgcctc ctgagtggtg
    15901 ggctttagat taagacatgt gctaccactg cctggctttt tttttaatgt gcattggtgt
    15961 tttgcttgca tgtatatata tgggaggggg ttccctggaa ctggagttac aggcattgtg
    16021 ggtgcttagg actgaatctg actcctttgg acgagtagct ggtgctttta accactgaga
    16081 catctctcca gtcccgggat agatttaata tatgagcatc taaaggtcga ggaccaagcc
    16141 tgacacctca ctgagccctg aaacatccaa aaatccacct ctaaaaatgc catcttcttg
    16201 atctctatgc agaactctct tctggcctgc gagtttctcc tccaaaatgg ggcgaatgtg
    16261 aaccaagcag acagtgccgg ccggggtcct ctccaccatg ccaccattct tggtcacaca
    16321 gggtagggag ccatggaaat ggcaccatgg ggaggaagct ttctgggagt gaaattgggg
    16381 gatgcctgtt gctgaattta gctctcttct ctaggctcgc ttgcctgttc ctgaaacggg
    16441 gtgctgatct gggggctcga gacacagaag gcagggaccc tctgactatc gcaatggaaa
    16501 ccactaatgc tgacatcgta accctgtgag tatgccgaag gggcagactg atgcttggac
    16561 agtcctcgtt ggggtgagac taacgtcaaa acctgacttg gagggtgagg tctggggtgt
    16621 gtgtgtgtgt gtgtgtgtgt gtggagaggg gtgtggagca ggagcatgga ccagttgggt
    16681 gagatactga gagttcacct ggggagccat tattaggaca ccaacattgc aaaaatgttc
    16741 gtgagtgtaa gcttttatct ttaagcacta gttatggaca gacattttta aggagtgaca
    16801 caactcaaga attggacaga ggatggggat ttggcgaagt tccaggtctt ttactcctta
    16861 atccccaccc ttcaggctac gattggcaaa gatgagggag gctgaagcgg cccagggcca
    16921 ggcaggtaaa gtaaccacgt atttcacccc gaccccgacc ccaggtccat accgaaatgc
    16981 ctgacccctt agtacccaac tgcattaata ccattgttta ttcaacaaac atgttttggg
    17041 cacagcgaac gtgcctgacc tgtggaacaa aggtactgta cttaggatgc acccacactt
    17101 taaagtgctt accctgccag gaccacccct actgtgtagc ccggctgtcc agcctcccag
    17161 tctttcctct gatttcctta ggagacgaga cgtatctcga catattccga gacttctccc
    17221 tcatggcgtc ggacgaccca gagaaactga gccgtcgcag tcacgacctc catactctgt
    17281 gattccaggc tcctccccac cctgccccat tgaagtctcc attaaagtct ctcagcttgg
    17341 cttctttctt agttcattta ctgtgcgcca cctgggggcc cggcagagtg tttggcacac
    17401 tggccaccca ggtgcgggta gccgtggtta ctcaagaggc tagggtttct ctaaaggagg
    17461 aagttgacta gcacccggtg gcttggcggg aggggctttc gagcccaggg gaccagggaa
    17521 gccaccgcgt gggaacctgg gctcgctggg atggcccgaa ggcatcccgg gaatgcgatg
    17581 gctcagcatc cgccccctcg ggagttcccg accgggatcc aacccggaga cgtggattct
    17641 ctcagactag aaggatggac tggagactcc atcctcgact aagttgtact agtgaggtca
    17701 tgcacatctg cagggaacgc ggggccgtcg cggccacatc cgcaacgaag cctgtgaatc
    17761 attccacccc cgctccagtc ggccggtcca gcctgttcgc cccatctctc gcactggcaa
    17821 ggccggctcc cacccatagc agagcccggc cagcccacgg gggcgtaggg cggggcgcta
    17881 aggggcggga ctgtcgctta aagggccact gaccgctgcc cttaggccac ttcctggggg
    17941 tggagaggac ctcagtgcct gctgcgacaa cccaggaagg cggtaacgcc cgagccctga
    18001 gacactgttc ccaacagagt cccgggacac tctcagctct gactgcaaga gcatcgcctc
    18061 tcccaagcca tacagaactg ctggctaccg gctcctatca gttgcgcctc catcttttgg
    18121 gacacacgcc cctaaccctg gctgggcctc tgaacaggtc ctcggaaccc gacgtggact
    18181 ctacaactac aaaagctcag aaactcaaac tgatcctttc agcgtccgac cggtgctcac
    18241 caccgtcacc tgctggttga atttcggaaa cccactgtct gaagagtaca ggagggaatc
    18301 gctgaccacg cacaatcaga agcgtctgga ccaaaagctc cctgatcctg tctggctgcc
    18361 gtctgttcct ttcatcggtt ctttcatcct ctcggggatt tttgtgtcct gaagccccca
    18421 accaccacca aagtagcttg ggtgagctac aaactttact gtcttgttct ccaacctctg
    18481 cctccaactt agcaagtctg aacgttacca cccaattctt tccaaacccc caaatatctg
    18541 ctttttcctt ctccttgcct gtctcttata ttacccaaaa tttccccccc tcctgtgtcc
    18601 tctttacccc cttcctttgg gggtcccgtg accctaaatg tggggggaac actatattcc
    18661 actacactgg agaccctgac tcgcttccca gattccatgc tgggggccat gtttagggct
    18721 gacaccctaa tgccagccaa ccttaacccg caaggagatg gccattactt catcgacagg
    18781 gatggcaagg ctttccggca catcctcaat tttttgcggc taggccgtct ggacctgccc
    18841 cgtgggtacg gagaaactgc ccttcttaag gcagaggctg acttctacca gatccggccc
    18901 ctcctggatg ccctgcggga attggaagcc tctcggggta cacctgcatc cacagccgcc
    18961 ctactccatg cagatgtaga tgtcagcccc cgccaggtgc acttctccgc tcgaaggggc
    19021 ccccaccact atgagctgag ctctgtccag gtggacacct tccgagccaa cctcttctgc
    19081 actgaccctg agtgtctggc tgccatgcgc aacagatttg gtgtggccat tggggacagg
    19141 gcagaaggag gtccacattt tcggctagag tgggcctccc gcccccagga actccctgaa
    19201 gtagagtatc aaagactggg gctgcagcca ctgtggactg gggggccaga agatcgtcgg
    19261 gaggtagcga acacgcctac attcctggag gaggtgctac gggtggctct ggaacatggc
    19321 ttccgcctgg actctgtctt cccagaccct gaagaccttc tgaactctag atccttgcgc
    19381 tttgtgcgcc actgagggtg ctgccctcca cttgactgca gaggaggggg atgagtcagc
    19441 caaggggcct gtggctcttc taaaacctct ttctagtact ggtgtggtag ctatgaaagt
    19501 cagggattcc accatacctg gccagagcct agagctgggg ttcccagatc gagctgcccc
    19561 cttccacccc acaagactca cgaatggtcc agaaggtcta cgcgggggct tatcctggga
    19621 aagcatcgtg gattctctag cctgtttttg cttgaggatg ggtcctctgt attcagaagt
    19681 gtggcaaagt aagatagcac ctctttgccc aggctaaacc tagcagagct ctggcgggtt
    19741 gagcactagg tgggaggact ttgacttatc aaatgtaacc tgctccctag actgtggttt
    19801 cctttactgg agctgacatc ttatcctaca gtaaccatta agaggccagg actctctggg
    19861 aggtttcttt actatgttcc aagccaccta ttgtgggaca ctccatccta gggtgacata
    19921 gggagcaagg agaacctcca tcaacacggg ggctagagct cccttctgga attccagagg
    19981 atcctgtatg gaagtggggc tggaccatgg tgtccagagg cctctttcat gtgtgtgcgc
    20041 ctgccagtca atagatctga tgctgcctgg ctccctgctg tcttggttcc catcagtgtt
    20101 gctgactcct gtgcttgtgt attggtcctt ctgtgatctt ggcctgtcca ggcctgtttg
    20161 gggttgctta gaaactctcc aggctcaggt acttctgtgg tatgtgtaaa agcagattca
    20221 gatttagttt aatcataggg tgtgtgtgta tgtgtgtgtg tgtgtgtgtg tgtgtttgca
    20281 tgtgtgttta tgcctgcgct gccacgtagg caggagggcc ttgtttgagt ttgagtcaca
    20341 ctaggccctt cctatgagag gtaaaagcct ctgggcttag ctgggcctct gagacctctg
    20401 ggagctcttg gttgctgaga caaccatgag cctgttgcta ggaaatagct gggtaaggaa
    20461 ggcctaaggc tgtcctgggc agccaggagc tagagttggg agagtcgggg agggtgtggg
    20521 aaggatagca tttctgggtt cctgggcgga tgggatacac acataaacat ggtcatcatt
    20581 gggtggagtc attcatctat cactgtgact tacaagaaga aaaataaatt gctttcagaa
    20641 ccatgacgtg taaaggccac tat
//