LOCUS       KO-first_condition_ready_72224_MGI:107448        24127 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Lyst targeting project(s): 72224
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 79899
COMMENT     backbone: R3R4_pBR_DTA+_Bsd_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     promoter        399..911
                     /note="PGK promoter"
     CDS             925..1581
                     /note="DTA"
     misc_feature    1706..1959
                     /note="BGH pA"
     promoter        2034..2100
                     /note="EM7 promoter"
     CDS             2101..2499
                     /note="BsdR"
     CDS             complement(4193..5053)
                     /note="AmpR"
     misc_feature    5272..5395
                     /note="R3 gateway site"
     misc_feature    5401..5408
                     /note="AsiSI"
     misc_feature    1..5433
                     /note="Synthetic Backbone"
     exon            8085..8175
                     /db_xref="ENSEMBL:ENSMUSE00000295925"
                     /rank="4"
                     /note="ENSMUSE00000295925"
     misc_feature    5434..10674
                     /note="5 arm"
     misc_feature    10702..10726
                     /note="B1 Gateway"
     misc_feature    10739..10786
                     /note="Frt"
     intron          10787..11661
                     /note="mouse En2 intron"
     misc_feature    11659..11661
                     /note="Splice Acceptor"
     exon            11662..11834
                     /note="mouse En2 exon"
     misc_feature    11846..12439
                     /note="ECMV IRES"
     gene            12440..15500
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    15539..15782
                     /note="SV40 polyadenylation site"
     misc_feature    15786..15819
                     /note="loxP"
     promoter        15846..16365
                     /note="human beta actin promoter"
     gene            16391..17185
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    17385..17626
                     /note="SV40 polyadenylation site"
     misc_feature    17643..17690
                     /note="Frt"
     misc_feature    17697..17730
                     /note="loxP"
     misc_feature    11676..11753
                     /note="FCHK std_gpr"
     misc_feature    17746..17766
                     /note="B2 Gateway"
     misc_feature    10675..17796
                     /note="Synthetic Cassette"
     exon            18198..20277
                     /db_xref="ENSEMBL:ENSMUSE00000379253"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00000379253"
     misc_feature    17797..20426
                     /note="Critical Region"
     misc_feature    20450..20483
                     /note="Downstream LoxP"
     misc_feature    20427..20506
                     /note="synthetic loxP region"
     exon            21477..22485
                     /db_xref="ENSEMBL:ENSMUSE00000295893"
                     /rank="6"
                     /note="ENSMUSE00000295893"
     misc_feature    20507..24127
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc
      421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
      961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
     1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
     1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac
     1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt
     1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact
     1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct
     1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat
     1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga
     1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
     1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac
     2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
     2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac
     5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac
     5401 gcgatcgccg gcggataaca atttcacaca ggacctactt tcccagtgta ccatcaaata
     5461 aaagaaaaat atttatggat ttcaaagaac agaattacat gaggagtttc tcttaagacc
     5521 agaaaggata aaaaccaaac aaacaagcaa aaaaccaaac caaaccaaac caaaccaaac
     5581 caaaccaaac caaaccaaac caaaataaga atatatggct ggcactttaa aaagtaatag
     5641 aatcagggct agtgaggtgg ctcagtggct cagtgggtaa agttgattgc aagagaagtg
     5701 tgtgctaggt ttgctctcca gaacttattc atgtgaaggt ggaaggagag aaacaactcc
     5761 tcacatctag acatatacta tggtatctgc acacacaaaa acacatacaa taactacaat
     5821 aacattatta ttattaggga gagctacacc aaaaataagt tgttttagaa atacagtaat
     5881 tgtatgaaat aaaaaattgt tgcattcagc tgctagtgta tcccttttct ccaccaggga
     5941 ctgttctagc atagcagggt caggccccaa aggtctgtac ttgctgccgt ctcatccttt
     6001 tcaagggttt gtggtctata agtctatggt gaactgaggg gaacaggagc ctcacagaat
     6061 agatattgct ggagctgggc tttgtatggt ggttccagag cgtgcagagc agcaggcggg
     6121 gcaggaatgt ctgttcacat agcagtgatg gtttgccagt gtactgttag agagcaagaa
     6181 ctcactcacc aggacagttt gttcccttag aatattgtgc ggccatccac taaagactca
     6241 aacttggaca actctgaagg attaacgagc taaaaagcta tttactttta gactaggaat
     6301 ctttctttaa ctcctttgac tctgcagctg aaacaggtcc taagcctctc atcattacat
     6361 ttcactccat taaagctgtg attgtgaatg ctaccagctc cctccagctg cttaaggctt
     6421 ttcagccatc ttacacagct cagaaaggcc agcttgcatc ctgtgaatgc cattcctttc
     6481 actgctctct tctgtggtgt ctctgacttc acatctctgt accagtctgg ttgtcaatct
     6541 gctcaatagg cttccttcag cactgcccac ccttacttcc ttgaagactc ttagagctca
     6601 gaatcccatt gtcatcagtt ctctcatcat tctgcttgct ctctggaaac ccttacttat
     6661 gtcctagaca tccacccaag tgattttcat agcttacgta gacagattac attagggaga
     6721 gctacctcat atatatacac acacacatat atatatatac atacatacat acatacatac
     6781 atatatatac atacaaacaa atatgttata tataattata attaaataac tatatattat
     6841 acataatata acatctccat ttagacacat taatgtctca gattatatat gtctaaaaaa
     6901 tgcatctggg ccagtgagat ggcttaggta gaggcatttg ctgctaagcc taacagtaca
     6961 ttcattaaat aaataacata taattaaaaa gattaagtca ctccatcatc tttagcctaa
     7021 cttagtatcc ctcttagatt ttctttcttg gtggagatat ttttggcacc tagttaccta
     7081 agccagaaac ctgtgaggag ttttttcctc acttgtacat cattgaaaat taactatgag
     7141 ccatttcagt tctactgtat agtttctcat atgtctgtgt gtctgtttgt tgtggggtca
     7201 ctttgtaatc attgtggccc tgctgttaac attggctgtt cctacttacc ccagttctgc
     7261 cttgaaaatc ttgccttacc ttgcttcctt ccccaaggaa tggtatgaga aggaataagc
     7321 cctggcttaa ctgttcatta aatactgggc cactcatgtc gcaagtgtcc cagcaatagc
     7381 tgtctagttg cagtccttct tcaacacttc tgatgattgt tcttattttc tgaactccta
     7441 aactgaatac cagttccata ctaaaattta gttcaatact agggagatac tatatgtttt
     7501 ctactgtcct gtcttgtgtg tatgtatttt atggtccaaa tagagacctt ttccaacatg
     7561 ggtcgtttac ttcaaaaaga ataatagagt gtgaaattaa tatatttaat tatcttcact
     7621 gataaatctt ttcatatgta ttattaattt tgctttcttt taaaatttgt tttttatttt
     7681 ttatatttaa ctacatgaag acttaaattt tgtagtaact ttgttattaa ttaatagctt
     7741 ataggattta attttttagt tttacttaac aagtctgttt tttttatagt gaggaattct
     7801 tgcaatgcgc atggtgtcag tagcagctct tcttgaagta ttagtgagaa tgtgctaaat
     7861 accatcacat aatcttacat tcttctgtgg tcagaaatgc ttataaattg aaaagggtcc
     7921 acagtttgac ttggacttca aataggttaa tttgatcctc ggttttcaaa tgtaaccttt
     7981 ttttttaagg aaaaattttg tttttgtttc agttaaatgt aggatgttag gaattaatgt
     8041 gtcttggggc cgtagaacat gaaagacttg tgtttctgac acaggccctg acatgcagag
     8101 aagaactcct gactcttctt ctgtcgctcc ttcccttggt gtggaagata cctgtccagg
     8161 aacagcaggc aacaggtaag tcctttttca caggtcacct gcattatcaa gtcccgatgg
     8221 gaaaagaagt tcattggcaa agctactttt gcctgatgag ttaccgcagc ttcagctgca
     8281 gagcagtaga tgctgtaggg atatttttat tatgtatctg gtatgaaaat acattaggca
     8341 agaacatttt tatttcttct agtaatataa aaactgtgat gcttcttaga ggcttacaca
     8401 aatgacaaat gcaaaataca tgaagaagca gagtacttag cgaaagaagt tactaggtac
     8461 gctgcattag gaagatgggt ggtgaagact ttgaagaaac aaattacagc tctttgctta
     8521 aagactgctg tcgacagtat tttctttagt tcaatttttc taattttgct tttgaagtca
     8581 tatgttgttt caaggctaaa tagaattatg aggtcaatag gtaattttaa ttttgttttt
     8641 tatattttgt tttagtttgg ttttagtttt ggttttggtt ttttgatgca tggtttcttt
     8701 gtgtagtcct ggctgtcctg gaacttgctc tgtagaccag gctggccttg aactcagaga
     8761 tcggcctgcc tctgcctccc aagtgctgag attaaaggca tttgccacca ttgttcagtt
     8821 ttcatttttg attttggtaa ctatgcatgt ataagcacat gagcctttaa gtacttcagt
     8881 ggaaatataa acaggtttct ccctggtttc cccctaaatt cagattctcc tggtttaaaa
     8941 ttatcttttt gtgtcatatt tttcttgctt atacttcaat gcatatctga catagagctc
     9001 tttttttctt attttaatat ttatagttca aatgttatct ccctttacag tcccccctcc
     9061 acagcctcac ccccacccca tcctcattcc gcattgcctc taagacagtg ctctcccacc
     9121 ccccccccca tccactccca tctcacccct ctgacatccc ctttctctgg ggcttcacgc
     9181 ctccacagaa ccaagcgcat ccccccctaa tgagaacaga caaggcagtc atacggacca
     9241 gcccagtata ctctttggtt ggtggcttag tccctgggag ctctgagggg ttcggttact
     9301 tgatattgtt gttcttccta tggggctgca atctccttca gctccttcag tctttccctc
     9361 aactgagccc atatctatct actgggaatt ggtctcttca ggttccatct ccccactttt
     9421 agggattttg gctgagatca tccccactgg gcctctcatg tccctggtgt ctgggacttt
     9481 ctagaggttc tccccatccc tcaccccaca ctgctgcata tttctattca ttctccgggc
     9541 cctctgggct tctctcccat ttccctccat acctgatcct gccccctctt ttttccctcc
     9601 tcttcccctc ttctatccag gtccctccct ccctctgcct cctgagggat tgaagcatcc
     9661 acaattgggc cttccttctt gttaaacttt aattggtctg tgagttgtat catgggtatt
     9721 ctgaactttt tggctaatat ccacttataa gtgagtacat accatgcatg tccttttggg
     9781 tctgggttac ctcattcaag atgatatttt ctagtttcat ccatttgcct gaaaaattca
     9841 ttgtttttaa tagctgagta gtattccatt gtgtaaatga acctcatttt ctgtattcat
     9901 tcttcagttg agggacatct aggttgtttc cagcttctgg ctattacaat taagtctgct
     9961 gtgaacttag tggaacatgt gtccttgtga tatggtgtag catcttttgg gtatatgcct
    10021 aggaatggta cagctggatc ttcaggtaga actatttcca attttatgaa tcaaggcata
    10081 cagctctttt aaggaagata tttaaagact tctaatatat tttcctgatt atttattgat
    10141 ccagtaactt gcttattttt tatgtgcaga attaaaaatt tttcaatcac ttgagaaagt
    10201 tgatttatgc catttgtggg aagttgattt cattgctggc atgttatatt ttggtggaat
    10261 tggtcccttt taatatttgc tcttatctgc tttgttttcc ctctcctaaa taaagtataa
    10321 gattctatta acctgtatta attctattta ttcctctgtt ttgtataact ccattttgta
    10381 tagactgcag taaatattat tgcaaaggga aagatgcatt tagttcgatc ctttctttaa
    10441 gaaatgacca ctgaatgctg ccccgagctc tgagggctaa ggatggactg ctgttctcta
    10501 cacctggctg atgtaggcaa caaggcccta tagaaataac tcttacagct ctcactgtga
    10561 gtctggcctc cagaattttc ctgcaaggct gttttccttg ttacatctct cagtaacgtc
    10621 agcctgcagt tatcctttaa atctcaagtc cgggtgccat taaacctacc atttaaggcg
    10681 cataacgata ccacgatatc aacaagtttg tacaaaaaag caggctggcg ccggaaccga
    10741 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcgaac cctttcccac
    10801 accaccctcc acacttgccc caaacactgc caactatgta ggaggaaggg gttgggacta
    10861 acagaagaac ccgttgtggg gaagctgttg ggagggtcac tttatgttct tgcccaaggt
    10921 cagttgggtg gcctgcttct gatgaggtgg tcccaaggtc tggggtagaa ggtgagaggg
    10981 acaggccacc aaggtcagcc ccccccccct atcccatagg agccaggtcc ctctcctgga
    11041 caggaagact gaaggggaga tgccagagac tcagtgaagc ctggggtacc ctattggagt
    11101 ccttcaagga aacaaacttg gcctcaccag gcctcagcct tggctcctcc tgggaactct
    11161 actgcccttg ggatcccctt gtagttgtgg gttacatagg aagggggacg ggattcccct
    11221 tgactggcta gcctactctt ttcttcagtc ttctccatct cctctcacct gtctctcgac
    11281 cctttcccta ggatagactt ggaaaaagat aaggggagaa aacaaatgca aacgaggcca
    11341 gaaagatttt ggctgggcat tccttccgct agcttttatt gggatcccct agtttgtgat
    11401 aggcctttta gctacatctg ccaatccatc tcattttcac acacacacac accactttcc
    11461 ttctggtcag tgggcacatg tccagcctca agtttatatc accaccccca atgcccaaca
    11521 cttgtatggc cttgggcggg tcatcccccc ccccaccccc agtatctgca acctcaagct
    11581 agcttgggtg cgttggttgt ggataagtag ctagactcca gcaaccagta acctctgccc
    11641 tttctcctcc atgacaacca ggtcccaggt cccgaaaacc aaagaagaag aaccctaaca
    11701 aagaggacaa gcggcctcgc acagccttca ctgctgagca gctccagagg ctcaaggctg
    11761 agtttcagac caacaggtac ctgacagagc agcggcgcca gagtctggca caggagctcg
    11821 gtacccggaa gatctggact ctagagaatt ccgcccctct ccctcccccc cccctaacgt
    11881 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
    11941 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
    12001 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
    12061 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
    12121 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
    12181 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
    12241 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
    12301 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
    12361 gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
    12421 tgataagctt gccacaacca tggaagatcc cgtcgtttta caacgtcgtg actgggaaaa
    12481 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    12541 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    12601 gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct
    12661 tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc
    12721 catctacacc aacgtgacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa
    12781 tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca
    12841 gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg
    12901 ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg
    12961 cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg agtgacggca gttatctgga
    13021 agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc
    13081 gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc
    13141 tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt
    13201 ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat
    13261 tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc
    13321 gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc
    13381 cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga
    13441 aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga
    13501 gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct
    13561 gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg
    13621 gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca
    13681 cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga
    13741 acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct
    13801 ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt
    13861 cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat
    13921 tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg
    13981 gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata
    14041 cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca
    14101 gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata
    14161 tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga
    14221 tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac
    14281 ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt
    14341 gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct
    14401 ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca
    14461 gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt
    14521 acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca
    14581 gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc
    14641 gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt
    14701 taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac
    14761 gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc
    14821 gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc
    14881 cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac
    14941 cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat
    15001 tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca
    15061 tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct
    15121 cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg
    15181 ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg
    15241 ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa
    15301 catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc
    15361 ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc
    15421 ctggagcccg tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc attaccagtt
    15481 ggtctggtgt caaaaataat aataaccggg caggggggat ctaagctcta gataagtaat
    15541 gatcataatc agccatatca catctgtaga ggttttactt gctttaaaaa acctcccaca
    15601 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    15661 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    15721 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    15781 ccggaataac ttcgtatagc atacattata cgaagttatg tttaaacggc gcgccccgga
    15841 attcgccttc tgcaggagcg tacagaaccc agggccctgg cacccgtgca gaccctggcc
    15901 caccccacct gggcgctcag tgcccaagag atgtccacac ctaggatgtc ccgcggtggg
    15961 tggggggccc gagagacggg caggccgggg gcaggcctgg ccatgcgggg ccgaaccggg
    16021 cactgcccag cgtgggcgcg ggggccacgg cgcgcgcccc cagcccccgg gcccagcacc
    16081 ccaaggcggc caacgccaaa actctccctc ctcctcttcc tcaatctcgc tctcgctctt
    16141 tttttttttc gcaaaaggag gggagagggg gtaaaaaaat gctgcactgt gcggcgaagc
    16201 cggtgagtga gcggcgcggg gccaatcagc gtgcgccgtt ccgaaagttg ccttttatgg
    16261 ctcgagcggc cgcggcggcg ccctataaaa cccagcggcg cgacgcgcca ccaccgccga
    16321 gaccgcgtcc gccccgcgag cacagagcct cgcctttgcc gatcctctag agtcgagatc
    16381 cgccgccacc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
    16441 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
    16501 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
    16561 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
    16621 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
    16681 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
    16741 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
    16801 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
    16861 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
    16921 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
    16981 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
    17041 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
    17101 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
    17161 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
    17221 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
    17281 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
    17341 gagttcttcg cccacccccc ggatctaagc tctagataag taatgatcat aatcagccat
    17401 atcacatctg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
    17461 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
    17521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    17581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgggg gtaccgcgtc
    17641 gagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc gtcgagataa
    17701 cttcgtatag catacattat acgaagttat gtcgagatat ctagacccag ctttcttgta
    17761 caaagtggtt gatatctcta tagtcgcagt aggcggctta agattttgaa ttatatgggt
    17821 gaatattgaa gttctgagac actataaact actctatgtc ttaattttaa cattactaaa
    17881 gatttctaaa tggtgagcac agcaactgga taacccagag tctcatattt tgaaatcaca
    17941 atgcaatata taggttcaac ttaggtctac tttcctaact cttccttgct attttcaaat
    18001 cagtttgtat cccctgagct aattccactt ggcattgaga attaaaaaga taagtgtggg
    18061 gagaagtgac ccagagatgc aactagggaa agcagccttg aagggaaatt atgccaggcc
    18121 agactcatgc cggttgtgag tcattgcctg tgtgtttaac agttactaac ctaagacttc
    18181 tttcttgatt tcattagatt ttaacctgcc actgtcatct gatataatcc tgaccaaaga
    18241 aaagaactca agtttgcaaa aatcaactca gggaaaatta tatttagaag gaagtgctcc
    18301 atctggtcag gtttctgcaa aagtaaacct ttttcgaaaa atcaggcgac agcgtaaaag
    18361 tacccatcgt tattctgtaa gagatgcaag aaagacacag ctctccacct ctgactccga
    18421 aggcaactca gatgaaaaga gtacggttgt gagtaaacac aggaggctcc acgcgctgcc
    18481 acggttcctg acgcagtctc ctaaggaagg ccacctcgta gccaaacctg acccctctgc
    18541 caccaaagaa caggtccttt ctgacaccat gtctgtggaa aactccagag aagtcattct
    18601 gagacaggat tcaaatggtg acatattaag tgagccagct gctttgtcta ttctcagtaa
    18661 catgaataat tctccttttg acttatgtca tgttttgtta tctctattgg aaaaagtttg
    18721 taagtttgac attgctttga atcataattc ttccctagca ctcagtgtag tacccacact
    18781 gactgagttc ctagcaggct ttggggactg ctgtaaccag agtgacactt tggagggaca
    18841 actggtttct gcaggttgga cagaagagcc ggtagctttg gttcaacgga tgctctttcg
    18901 aaccgtgctg caccttatgt cagtagacgt tagcactgca gaggcaatgc cagaaagtct
    18961 taggaaaaat ttgactgaat tgcttagggc agctttaaaa attagagctt gcttggaaaa
    19021 gcagcctgag cctttctccc cgagacaaaa gaaaacacta caggaggtcc aggagggctt
    19081 tgtattttcc aagtatcgtc accgagccct tctactacct gagcttctgg aaggagttct
    19141 acagctcctc atctcttgtc ttcagagtgc agcttcaaat cccttttact tcagtcaagc
    19201 catggattta gttcaagaat ttatccagca ccaaggattt aatctctttg aaacagcagt
    19261 tcttcagatg gaatggctgc tttcaaggga cggtgttcct tcagaagctg cagaacattt
    19321 gaaagctctg ataaacagtg taataaaaat aatgagtact gtgaaaaagg tgaaatcaga
    19381 gcaacttcat cattccatgt gcacaaggaa aagacaccgg cgttgtgagt attcccactt
    19441 catgcagcac caccgcgatc tttcagggct cctggtttca gcttttaaaa atcagctttc
    19501 taaaagcccc tttgaagaga ccgcagaggg agatgtgcag tatccagagc gctgctgctg
    19561 catcgccgtg tgcgctcacc agtgcttgcg cttgctgcag caggtttccc tgagcaccac
    19621 gtgtgtccag atcctatcag gtgtacacag tgttggaatc tgttgttgta tggatcctaa
    19681 gtctgtgatc gtccctttac tgcatgcttt taagttgcca gcactgaaag ctttccagca
    19741 gcatatactg aatgtcctga gcaaacttct tttggatcag ttaggaggag cagagctatc
    19801 accgagaatt aaaaaagcag cttgcaacat ctgtactgtg gactctgacc aactggctaa
    19861 gttaggagag acactgcaag gcaccttgtg tggtgctggt cctacctccg gcttgcccag
    19921 tccttcctac cgatttcagg ggatcctgcc cagcagcggc tctgaagact tgctgtggaa
    19981 gtgggatgca ttagaggctt atcagagctt tgtctttcaa gaagacagat tacataacat
    20041 tcagattgca aatcacattt gtaatttaat ccagaaaggc aatgtagttg ttcagtggaa
    20101 attgtataat tatatcttta atcctgtgct ccaaagagga gttgaattag tacatcattg
    20161 tcaacagcta agcattcctt cagctcagac tcacatgtgt agccaactga aacagtattt
    20221 gcctcaggaa gtgcttcaga tttatttaaa aactctacct gtcctactta aatccaggtt
    20281 tgtatgtgtg tatatgtttt acagttgaat gagtgctttt gaacacggga tgaaatgtgt
    20341 atatttgaat ttctgacctc cagggactca accatagtat aatcatagta actgtattgc
    20401 tatcaagaac tttagtttgt caacaagaga tggcgcaacg caattaatga taacttcgta
    20461 tagcatacat tatacgaagt tatggtctga gctcgccatc agttcaccat ttttaaacat
    20521 tattttaatt aacttttctg gaggtagtta gaggacagaa gctagagaca ttgattttta
    20581 gcttctcagt gtgtgtgtgt gtgtgtgggg gggggggcgg tgaaatatat ttttggttat
    20641 atttctgaag gaaagaaatg tgtgagacaa agatatctaa gaaaaggcca gtttaaatga
    20701 atggttttgt atttataatg tagattgcat attaaagtcc attaaaatgt ttataaagat
    20761 catgttagga caggaaaaga gcttgtatta ttttatgagt agcacataaa gcttaaagta
    20821 gtatttatgt atttatgacc gtgtaaaatt tgcacataca tgaactattg ggttcctgag
    20881 tcagtggcag caggagatac tttcttaaat gccacagtca tgtttattac tcagacagag
    20941 tagaaaatac agataagcaa aaagaaagtc taaattgtat tttatcaatg aattttccaa
    21001 tttttgtgat tgttaatttt ggagcagtta agattgttaa cttgggaagt cctgagcaga
    21061 gtgttagaga accaagggag caagtgaagg gaatatatac ctgccctccc agtgatgatt
    21121 tgatatttag aatttctatg aaatttatta tttgaagaca aagttctttt ttgagttgcg
    21181 tcttttaatg tgatatatat atataatata tgtatatata tatatatata tatatatata
    21241 tatatatata tataatttct ttttaagtac ttccagagct gtatatgagc atataagtgc
    21301 tattacaaag taacttgaga tcttgatcag tatcttagaa ctatcaaata atgaaccaga
    21361 gtaggcagtg tttggtgtat ataccacaca cagcaatagc atgtttacat aacatattta
    21421 cctatttata aatgtttcag tatgtcatat aaaaatcttt ttttcctctc tcacagggta
    21481 ataagagatt tgtttttaag ttgtaatgga gtaaaccaaa taattgaact aaattactta
    21541 gatgggattc gaagtcattc cctgaaagca tttgaaactc tgattgtcag cctaggggaa
    21601 caacagaaag atgctgcagt tctagacgtc gatgggttag acatccaaca ggagttgccg
    21661 tccttaagtg tgggtccttc tcttcataag cagcaagctt cttcagattc tccttgcagt
    21721 ctcaggaagt tttatgccag cctcagagag cctgatccaa aaaaacgaaa gaccattcac
    21781 caggatgttc acataaacac cataaacctc ttcctctgtg tggcttttct atgtgtcagt
    21841 aaagaagcag actctgatag ggagtctgcc aatgagtcag aagatacttc tggctatgac
    21901 agcactgcca gtgagccatt aagtcacatg ctaccatgtc tgtctcttga gaacgttgtc
    21961 ttaccttccc ctgaatgttt gcaccatgca gcagacattt ggtccatgtg tcgttggatc
    22021 tacatgttga actcagtctt ccagaaacaa tttcacaggc ttggtggttt ccaagtgtgc
    22081 catgaattaa tatttatgat aatccagaaa ctattcagaa gtcatacaga ggatcaagga
    22141 agaaggcagg gagaaatgag tagaaatgaa aaccaagagc taatcaggat atcttacccc
    22201 gagctgacac tgaagggaga tgtatcatct gcaacagcac cagacctggg atttctgaga
    22261 aagagtgctg acagcgtgcg tggattccag tcacagcctg tgcttcccac aagtgcagag
    22321 cagattgtgg ctactgaatc tgttcctggg gaacgaaagg catttatgag tcaacaaagt
    22381 gagacttctc tccagagcat acgacttttg gagtctctcc tggccatttg tcttcatagt
    22441 gccagagcct gtcaacagaa gatggaattg gagctaccgt ctcaggtgat actttgtact
    22501 tagtccgtct cacataacat atatgtgtat tatatgtact gcatgtacat ataataatat
    22561 ataatataca tgttctaact taacactgtc tattttgcca caaatgttaa atttattttc
    22621 aagtattaat tacatataaa actggaaatc ataatgacag ttttatgtat atagtgtatt
    22681 tatatcaatt taccctcctt gccttctctt tcttccctcc cactcatccc cttccttatc
    22741 ccagctagtt tcctttctat ttcatatttg ttgttgttgt tgtttgggct gtgtgtgtgt
    22801 gtaaaagccc actacattgt attagggttt cttgttaaat cctagatgaa gaattattta
    22861 ccagagcacg agttcctcgc cagtggttat atcacaggag aaaaatgtct ttcccttccc
    22921 caggaaccat tcactcctta gaggttctaa aagggctggg cctcaggagc tctttccctt
    22981 gctaccattg tctgcctatt aaatgcacag ggaagggagg ggaagggcct cagggccctc
    23041 cgcacttcca tttgctacct actttgtttt tagaaaaccc ttttgtgctt accagaatac
    23101 caagtatgga ggacacaagg gacacacagt tcctactcga aagaagctta ctgtttaatt
    23161 ttctgcaaaa gcccctttag gagagtttgt gaattcagtg ttaaatgagt agttgaggtc
    23221 attttgtttt agagtttaga agttcataat cttggtgtta ctgttgggaa ctctgcccat
    23281 attttctgac ctttgtttat ggttttgttg tggttatcat ctggtctgta cctatttgtt
    23341 ttacttttta gacttggcat tccttcaaag aaagataagg ttttttgttt tttaatctac
    23401 tgttatagag agctaggcaa gcgtaatagc ttcattcaaa gggtagaact gaaggcaaaa
    23461 gaaaaaataa ttagaactgc ttcttagaat cttagagaac ctttaagaat aaggaatttg
    23521 taaactctag agataactaa tattctgaag tttcaaagaa tgacttggta aaaatttaag
    23581 atttatttat ttttatatgt atgctatttt gcgtggacat atgtctgtac actatgtgtg
    23641 caaagtgtct ggggaggtca aaagagaggg tattggattc catggaactg cagttacaaa
    23701 cagttgtgtg ctgccataaa agtgttggaa atggaatttt ggtcctctgc aaggactctt
    23761 aaccactgag ccatctcccc agcctccaaa aagtggaatt atattttttt caagacaggg
    23821 tttctctgtg tagtgctggc tgttctgaaa cttgctctgt agaccaggct ggcctgaact
    23881 cagagatcca cctgattctg cctcatatgt gctaggatta aaggctttta ttaccaccac
    23941 cctgctaaga gtggattttt tatgattaaa cagaatttta acagtacatt atttggataa
    24001 gaactcagat tttttttttt ttgcagtcag tttaccttca tattaattac aaataaatgg
    24061 atttttccct gcttcttgta atcctttctg tcacttttaa aatgtgtgtt attgatattc
    24121 ttagtct
//