LOCUS       KO-first_condition_ready_34134_MGI:2178217        20029 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Akap9 targeting project(s): 34134
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_gt0
COMMENT     design_id: 37213
COMMENT     backbone: L3L4_pZero_DTA_kan
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     promoter        85..598
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             612..1268
                     /note="DTA"
     misc_feature    1393..1651
                     /note="BGH pA"
     CDS             complement(2147..2941)
                     /note="KanR"
     misc_feature    4053..4073
                     /note="B3 Gateway"
     misc_feature    4079..4086
                     /note="AsiSI"
     misc_feature    1..4111
                     /note="Synthetic Backbone"
     misc_feature    4112..8921
                     /note="5 arm"
     misc_feature    8949..8973
                     /note="B1 Gateway"
     misc_feature    8986..9033
                     /note="Frt"
     intron          9034..9908
                     /note="mouse En2 intron"
     misc_feature    9906..9908
                     /note="Splice Acceptor"
     exon            9909..9948
                     /note="mouse En2 exon"
     misc_feature    9943..9950
                     /note="Frame 0 insert"
     misc_feature    9951..10004
                     /note="'self-cleaving' 2A peptide"
     gene            10005..13082
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13083..13136
                     /note="'self-cleaving' 2A peptide"
     gene            13137..13928
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    14127..14369
                     /note="SV40 polyadenylation site"
     misc_feature    14386..14433
                     /note="Frt"
     misc_feature    14440..14473
                     /note="loxP"
     misc_feature    9923..10024
                     /note="FCHK std_gt0"
     misc_feature    9923..9940
                     /note="FCHK QC prefix region"
     misc_feature    9941..10006
                     /note="FCHK QC critical region"
     misc_feature    10007..10024
                     /note="FCHK QC suffix region"
     misc_feature    14489..14509
                     /note="B2 Gateway"
     misc_feature    8922..14539
                     /note="Synthetic Cassette"
     exon            14960..15173
                     /db_xref="ENSEMBL:ENSMUSE00000505652"
                     /type="targeted"
                     /rank="8"
                     /note="target exon 1 ENSMUSE00000505652"
     misc_feature    14540..15376
                     /note="Critical Region"
     misc_feature    15400..15433
                     /note="Downstream LoxP"
     misc_feature    15377..15456
                     /note="synthetic loxP region"
     exon            17043..17116
                     /db_xref="ENSEMBL:ENSMUSE00000503624"
                     /rank="9"
                     /note="ENSMUSE00000503624"
     exon            18281..18416
                     /db_xref="ENSEMBL:ENSMUSE00000381275"
                     /rank="10"
                     /note="ENSMUSE00000381275"
     exon            19135..19220
                     /db_xref="ENSEMBL:ENSMUSE00001224598"
                     /rank="11"
                     /note="ENSMUSE00001224598"
     misc_feature    15457..20029
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagataaacg
       61 gatcccctag tttgtgatag gatcgaattc taccgggtag gggaggcgct tttcccaagg
      121 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      181 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      241 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      301 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      361 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      421 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      481 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      541 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      601 aggtcctcgc catggatcct gatgatgttg ttgattcttc taaatctttt gtgatggaaa
      661 acttttcttc gtaccacggg actaaacctg gttatgtaga ttccattcaa aaaggtatac
      721 aaaagccaaa atctggtaca caaggaaatt atgacgatga ttggaaaggg ttttatagta
      781 ccgacaataa atacgacgct gcgggatact ctgtagataa tgaaaacccg ctctctggaa
      841 aagctggagg cgtggtcaaa gtgacgtatc caggactgac gaaggttctc gcactaaaag
      901 tggataatgc cgaaactatt aagaaagagt taggtttaag tctcactgaa ccgttgatgg
      961 agcaagtcgg aacggaagag tttatcaaaa ggttcggtga tggtgcttcg cgtgtagtgc
     1021 tcagccttcc cttcgctgag gggagttcta gcgttgaata tattaataac tgggaacagg
     1081 cgaaagcgtt aagcgtagaa cttgagatta attttgaaac ccgtggaaaa cgtggccaag
     1141 atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa tcgtgtcagg cgatctcttt
     1201 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     1261 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     1321 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgca
     1381 gatcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1441 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1501 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1561 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1621 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgacgcg ccgtacccag
     1681 cttttgttcc ctttagtgag ggcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     1741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2101 ctattggtta aaaaatgagc tgatttaaca aaaattttaa caaaattcag aagaactcgt
     2161 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2221 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2281 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2341 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2401 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2461 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2521 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2581 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2641 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2701 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2761 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2821 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2881 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2941 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     3001 ttggcggcga gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3241 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3481 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3961 ttttacggtt cctgggcttt tgctggcctt ttgctcacat gtaatacgac tcactatagg
     4021 gcgaattgga gctaggcggc cgtttatcgt gccaacttta ttatacaaag ttgcggacgc
     4081 gatcgccggc ggataacaat ttcacacagg attgagagac ttggtctcat tttccattat
     4141 aattactttg ctttagtatc tattatcttt ttagagtatt tcatacttgt gttttctagt
     4201 ataaaatttc aagttttcag ttagtatccc acaactgtaa aacaaaacaa aaaacctcct
     4261 aagttttcta ctgctctgta aagtactcag ttgttatgaa attatatttc tgagaggtga
     4321 ggcttaaggc cttaagtttt tgagtcttat acttgaatga gaaaaaaata ccgttaattt
     4381 tcaatataag tcaagatctt aacaatacct ataataaatg ttctagtttc atcctgttgc
     4441 tgtggtaaag ctgaccagaa gcagcttagg agaggaaagt gttgtgattg gcttacacgt
     4501 cctggtcaca gtgaagtgag ggtgaaaact cagtccgaac accatagagg agtggcgcct
     4561 cctggcttac ccactggctc atgcttgcct acctttctgg tatagcttgg gacttcctgc
     4621 ctacataatg gtgctgtcca caggggctcc cacatcaatt aacaatcaag atgttcaccc
     4681 atatatgccc actgaccagt ctaatctgag cagtatttga attgagacct ttctcatctc
     4741 tttgttgatt ctagactgtt gtcagtaatg ctaacaaaga ccgtgactaa taatgagtca
     4801 ttcctaataa cttatatttg gtaaatacta atgctagaaa aaccaggaca gttctggtag
     4861 gttatctaca gtataataat atgtgttttt atattagtgg aatatatgtt tattgaatta
     4921 ttctagatac acatatagtg gtgaacaaga gatgaggact agtctcagga agcttgctct
     4981 caaggggaaa gcagttagta agggggaagc aaagggtagt tagaaccagt gtcaggtctg
     5041 tgaagaagta cagggctagg caggagagta cagagatcag gacaggtctt ttggaggagt
     5101 tagcctttga actgagctct gtataatggg gaatttatag cagttgcaaa gatgtgggtg
     5161 agcgttagga tccaggccta tgtggttggc tctgaaaaga ggccaaaggc caaacagagc
     5221 tgtggaagga tggaaggatg caggaagctc agttttcatt ctaagtacaa gccaggggat
     5281 gtttgtcttt atttctgatg acttaatccc attgacaatc ttatatcaaa tacagagaga
     5341 atttgctttt ataataactt ataataacct gaataggatc aagaaattca ggctggagag
     5401 atggcttagc ggttaagagt gttggttggt tcaagagggc cccagttcaa ttcccagtgc
     5461 ccacatggca tctcacaact gtctaactcc agttccaggg gatctaacac cctcacacag
     5521 acatactgca ggcaaaacat caatgctcat aaaaataaat aatattttta aaagaatcaa
     5581 gtaattcaaa ttactaatta atgtctaaac acatggaatt gttttgaaaa atatgtgtta
     5641 taattacccc aaaggtgttc aacaacattt taattttttt acataaggga ggatctgatc
     5701 tccaacgtta gctgctctgc tcttgatact gtctctcact aaaattaaat aaatagagcc
     5761 acataggctt tcttttcagt ggaaacaaaa tacacttgaa gtgtgacttc tttatttgaa
     5821 tatacttgga taaaacgaga aagagaaact tcacagaaag tctccctagt gttggcatgt
     5881 gaaggtctcc agggctgtgg ccatgcggag gcagacaggg catgacagga attgctcatt
     5941 tcttatgctc gcctagtgaa gcatgtttga agtggaagac acggaactta gtgagcccag
     6001 gaggagctgc cagtttgctg tctctcaggt cctgccctgt gggcaagacc tcaggaacct
     6061 agagtaaatt tgatacaaga tagtacattt ttaagcagga aaataaagcc catttttcaa
     6121 gtgtcaatag tggaacaaag tagaactcct cacaggaaag aagagacttt gttagttgct
     6181 ttatagcatt ttccaaaagt cctaaccatt gtaaatataa aaagatatac gacatcagtg
     6241 gagagaaaga caactgtcat aattcacagg gacaatttaa taagaaagta agcaaattga
     6301 gttaaaaaat ttgaagtaaa atagtgtaaa aaaaaacccc aaaaatgtgt aatatacctg
     6361 ggtgtggtag cttatgccca taatcccagg acacagaagc agacagaggg aactgtgaat
     6421 tcaagagcag actcaggcat gtagcaagac ctcatcctaa aatctaaaca gtgacagctg
     6481 cgttgtacgt gaatgaagga gggtgaggcc tgaataaacg aaatctctca cttctggagg
     6541 aaaatattta atgtgagata taagtttgtt ttatgtgtct gcaatccagt tcacagtgaa
     6601 aacttgctaa gctggttgga aagataaaat agcatccaag tagccacact tagcgaaagc
     6661 agatagaaga aaatagaaaa tttagagcag aaataagtga atttgaaata accaaaaggg
     6721 aagggtttga agacacttga tggtttatta tttgaaaaca tctataggtt gcagttgtta
     6781 gattcccaag gatgtcacca agaaagagaa agcacagata aaatgaagga tggaaataaa
     6841 atccccagcg caaatgctac aaggatttta aatataaaaa tactataaat aaggcctact
     6901 gatgaaaaat ataagttgcc caaactaatt gcatagaaat gcaaagcttt agtgagctaa
     6961 tggcactaca gaatctagaa ctgtggtcgg cttcccttct cacctctcca tgtaaatgtg
     7021 ctctgctgtg accactgtgc agtgtgcggc ggctctcagg agctcacggc tgagcgcaga
     7081 gccttactga ggtggcatta aaagagaatt ggagggatgg aaggattcaa gctttgagtc
     7141 tggttggtat tgagataaag atgggaaaag aatggctcaa gaaaaagaaa attacagatt
     7201 attcttattt atgaatgagc ctgcctgaat tcttattaag ttattatgag attatcccta
     7261 agaatggaag aattattcgg catgtgataa ttctgtttac tcaatctcca catgctgatc
     7321 atcttaaata ctgttggaaa agaatctcca aaagttacca atttgtttcc aggttttaac
     7381 aggaataaca agaaggaaag aaactttgaa gaagtaagga ctagaaggag cagttgtgaa
     7441 ggttttctga ggtgttgttg agtagctttc ccggtgtagg cacggcaggg acagggcagt
     7501 cactgtcacc gttactcttc caacagtgct tctagagttc ctagccagcc aatacaatac
     7561 aaataagaaa ggagaaagac tgtgaacaag taagttaaaa atgttaaatt atagataaga
     7621 ttattgaaac ttacacaaga cattaatgac ctgtacccgt tgccacaggc tctctttata
     7681 cactttagtg taaagggata ccccaaaggt ctgtacctcc ttctgtctcc atctccatct
     7741 ttatctctct ctgtctctgt ctctcgtaca gagttagttc atgggagggt attctggtga
     7801 gataatattt gagaagagac ttgaatcaac aagccaagac aatgggaagg ctcagaaaat
     7861 tcaatgattg ccttaagaga attgtttttg cgatggggaa ttatatcctt actcttagga
     7921 aaaattaact ccaggttagt taaagacata ctagacagaa ggatgtaaat aaagtagaat
     7981 tgtgatctta agacgtagga agccttttga ttaagaagag caaagtcaaa ggaaagtatt
     8041 tagaaatttg atcatgtttg tttcctttat gaacaatact taaaacatgt ttcatactag
     8101 ggccagatgt ttgccacata tgttacaaac aaaggactgt aaatgtaata taaatacctg
     8161 agaaaataca aaggatttaa tgagccaaag atatgaagaa atatgtgtct tagagaagaa
     8221 accaaaatga caaggaacat aaaaagtatt ttagccttac aaattcatat gagtaaaata
     8281 gcattttatc accatcacag tggcaaaata accgacttat tgataaggct acaaggatgt
     8341 aagtatccac ttttttttag ttgaattata caaaacaatt atttagctac taaagctaag
     8401 cctgcgctat gaccagcagt tacattcctg agtaactgtg gtcagtggga agcagctacg
     8461 cttggaaaca ggcagcactc gtgtttctta agcttcctga atcagggtca gaggacagat
     8521 accatagatg tgtgtcagcg agaattccag ttgagtctga tacatgctgg accgctgacg
     8581 agcagagcct ttactcattt gtctcaggtg gatgtatgta atcatttact accttttgat
     8641 taaaggaccc aggcttatga aaatccagtt gggctccgca aaggaaaacg gtccagttgt
     8701 ggattgttca tttatactac actatagtca aaataaatta acctgtcaca tatgtggatt
     8761 aatttcagaa gcattttttg atgttttgat atttgatact aaaatgcaaa gaaatttgca
     8821 aaataaatgc ctctgtgtca atacacagaa atatacaata taatgtaatg tttacatgtt
     8881 cactgggaaa tgatagtaaa caccttccag atttttggta aaaggcgcat aacgatacca
     8941 cgatatcaac aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt tcctattccg
     9001 aagttcctat tctctagaaa gtataggaac ttcgaaccct ttcccacacc accctccaca
     9061 cttgccccaa acactgccaa ctatgtagga ggaaggggtt gggactaaca gaagaacccg
     9121 ttgtggggaa gctgttggga gggtcacttt atgttcttgc ccaaggtcag ttgggtggcc
     9181 tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt gagagggaca ggccaccaag
     9241 gtcagccccc cccccctatc ccataggagc caggtccctc tcctggacag gaagactgaa
     9301 ggggagatgc cagagactca gtgaagcctg gggtacccta ttggagtcct tcaaggaaac
     9361 aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg gaactctact gcccttggga
     9421 tccccttgta gttgtgggtt acataggaag ggggacggga ttccccttga ctggctagcc
     9481 tactcttttc ttcagtcttc tccatctcct ctcacctgtc tctcgaccct ttccctagga
     9541 tagacttgga aaaagataag gggagaaaac aaatgcaaac gaggccagaa agattttggc
     9601 tgggcattcc ttccgctagc ttttattggg atcccctagt ttgtgatagg ccttttagct
     9661 acatctgcca atccatctca ttttcacaca cacacacacc actttccttc tggtcagtgg
     9721 gcacatgtcc agcctcaagt ttatatcacc acccccaatg cccaacactt gtatggcctt
     9781 gggcgggtca tccccccccc cacccccagt atctgcaacc tcaagctagc ttgggtgcgt
     9841 tggttgtgga taagtagcta gactccagca accagtaacc tctgcccttt ctcctccatg
     9901 acaaccaggt cccaggtccc gaaaaccaaa gaagaagaac gcagatctcc gagggcagag
     9961 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctgggatc tggactctag
    10021 aggatcccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    10081 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    10141 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac
    10201 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg
    10261 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc
    10321 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca
    10381 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg
    10441 ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc
    10501 gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg
    10561 tgatggtgct gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga
    10621 gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc
    10681 atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga
    10741 tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc
    10801 aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg
    10861 ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc
    10921 cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag
    10981 aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg
    11041 gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg
    11101 tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg
    11161 ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg
    11221 gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc
    11281 tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc
    11341 gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg
    11401 ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt
    11461 atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg
    11521 tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc
    11581 tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc
    11641 ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct
    11701 tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt
    11761 cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc
    11821 tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt
    11881 ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc
    11941 atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg
    12001 gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac
    12061 cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga
    12121 ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc
    12181 tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg
    12241 atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt
    12301 cacagatgtg gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc
    12361 gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct
    12421 gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca
    12481 cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg
    12541 ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga
    12601 ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact
    12661 gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact
    12721 atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt
    12781 ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt
    12841 atggcccaca ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc
    12901 aactgatgga aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata
    12961 tcgacggttt ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg
    13021 aattccagct gagcgccggt cgctaccatt accagttggt ctggtgtcag gggatccccc
    13081 gggagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctattg
    13141 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
    13201 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
    13261 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
    13321 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
    13381 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
    13441 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
    13501 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
    13561 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
    13621 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
    13681 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
    13741 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
    13801 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
    13861 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
    13921 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
    13981 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    14041 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    14101 cccggatcta agctctagat aagtaatgat cataatcagc catatcacat ctgtagaggt
    14161 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    14221 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    14281 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    14341 catcaatgta tcttatcatg tctggatccg ggggtaccgc gtcgagaagt tcctattccg
    14401 aagttcctat tctctagaaa gtataggaac ttcgtcgaga taacttcgta tagcatacat
    14461 tatacgaagt tatgtcgaga tatctagacc cagctttctt gtacaaagtg gttgatatct
    14521 ctatagtcgc agtaggcgga attaaatctt gtctctaatg ttttctttct aaatctaagg
    14581 agctgtagat ggaataggag aaaaaaaaat gacagcattg agaattatta atttgagttt
    14641 tgagggcatt ggtgtttatt cagattattt ttattttgta gcatgtaaaa tattagttaa
    14701 aagattaaat ttttgctagt tataccaact atcccaactt acaattacac tgagttagta
    14761 aatgaaaaca tgacaaggcc tttcttgaga aagacatgta ggtcgggcat ctggatgaag
    14821 actataaagc aggccagttc cttcctatga cttttaaaac tatgacatgt cttctccttt
    14881 gaagtccagt ccatggaaag aagcagaagc taagctgtta ctttcatttt ccctcacgtt
    14941 tgtacctctt ccctttaagg atgatctaag gctgcagatg gaggcccagc gcatatgcct
    15001 ttttgtggtt tactcaactc atgcggatca ggttcgtgca cacatggaaa aggagaggga
    15061 ggaagctctt tgcagcctta aagatgagct catttccgct caacagaaaa agatcgacga
    15121 acttcataaa atgcaccagt gccagctcca gaattttaaa atacaggaga caggtaaccc
    15181 atcggcttct gactaatttc tatgaaacaa ataagcaccc ttaaaggcac ctttttgtag
    15241 ttctgtccct gtagttctgg catgggctga ggaggctttg cgtctgttgc ccgggtttgt
    15301 taattcatgt cattgaggtg ccaggatagc caaacccctt cctgcctcat tttctcacgg
    15361 aagtataatc cattcagaga tggcgcaacg caattaatga taacttcgta tagcatacat
    15421 tatacgaagt tatggtctga gctcgccatc agttcatctg tctggggcag atgtacacca
    15481 ttggctctac tgttacttga agtattccag actaacgaat aatccagctg atccagggtg
    15541 aatactgaga gtggaaggaa gaggagagga gaagaagttc tgtgaagatg gagcaggatc
    15601 agttgggttg ggcactgagg gatgtttctg aagtgttaag catagacctg aaagagaccc
    15661 actttggaga agtgaaagtt caagttgtat agaaagtagt ggggcatgag ctgaagattg
    15721 tggcgcgtgc ctggttagaa gcttcataaa tctcaacgga gtacactcat gcttcctgct
    15781 ctgggaagac agagctactc gatctactgc agggtttgta aggagagcaa gcaacagtga
    15841 cactcttatt taagaggaca ctagtgcgct tagttgagaa aagtgaaata ataatgggca
    15901 gagcagctaa gcaatcttag ctataaccaa taaatgtctg gaaacacagt gacagtgaag
    15961 ctcaacagtt agagctaaat gctatcacgg ggcaatttat agcagcaggt ggtagaagtt
    16021 gagaataatc ttgaagtttc tgcctatgta gtttcagtca caaagaggag cagaccatag
    16081 acaaataata tagtcagttt aggacattct gagttaagtg tatgtgttaa cttgtaaaag
    16141 aaatagtcac tagtcagctg aagggagtag tctaaaaccc aggagagact tttaggctaa
    16201 aaatacgatc tacatggagc tgaaagacca gagtctctaa ggccagaagt agagtgagac
    16261 accctggaga tggagcagaa aaccttggtg gcgcggtggg aaggacattg aagagaccac
    16321 agaggaaggc agaacctgtt atggggcagc tgttgttgag gaaacccagt gtttcctaaa
    16381 tgtccagtgg aattgctctg ttgaacaaag ttaagatttc aagagtcact taaagaagga
    16441 gagtttttca tttacagagt tctttgcttt ttttttctaa attcattaag attttgcatg
    16501 cattgcttgt attgatacct cttctgatag cctgtctagt gttttctatt gacagtcgat
    16561 tgttctatta ctaggtgaca agacagtcta gtgagtagtg atcaatgtct ctgagaagag
    16621 aagagaacag aaagtatata tctaataaat aactaatgga tttaatgttg caatactagg
    16681 actgaaatcc agggctagca catactaatc aaagtgtcct atctctgaac tctgactctg
    16741 caaaattgga accactggct tgtgagagtt ttgaggtttg caaaggtgta tttaatggat
    16801 aagtaagtat ataggttctt gggttagaat gtagaatcct gaatgtataa acccttctat
    16861 agagtcgaaa tagcaaatct tagaaatcta tctgagacag ctgcattgca aaatgaaaaa
    16921 cacatttgga agaaattgaa atatgtgata ttctagggaa gctccggtga aggcattaga
    16981 atgtgacagt gttacttaca tatatgcagc aaatggctca cgtgtggtgt gtttcctgct
    17041 aggtgatgag cctttacagg tgctcattga acgacttcaa caggcagtgt ctgagaaatg
    17101 ctttcacatt tcaaaggtaa gatgttcctt taaaagtggt tttagttggg aggtaccaaa
    17161 atccatattg ctactgattt aatgaacaga accatccact cacgtttgcc caaatctctt
    17221 gcgtccagaa ctgtacttac aaagtattcc tgagttctaa gcttaaaatg agcatttgcc
    17281 ttttttgtga tagtcagatt ggaacaatag gaaattagag ataagggtgt gactctgatc
    17341 cattgcttgt ctgttatatt tgaggtcttg gattcagtct ctggcaccac agaaaaagaa
    17401 caaagaagtt gagataagtt gtatctcgaa tagtgcacac tgtatagtca ctgttcgtgt
    17461 cagagaggta ctgtttaacc ttccaggaaa acagtttgtt cattaaggtc actaatgtgg
    17521 taaaagttta ccttgtggct gtgctgaaat ctcagtgtgc ccaaatactg acagtgtcct
    17581 taacttaaaa tcatgcagga agcaacagcc atttaaaaca ttggctttta aaagtcttct
    17641 aaatcattgg ttatttccaa ttgagagagg ggcaaaggta aagttaactt ggacagctta
    17701 tcaaaccttg gaacatgaaa tggtgtctta catgatggaa atttcagttt ctctggagaa
    17761 cagtgtgctg gacactttta actttttgta tgggcagacc acagaaaaca ctctgcatat
    17821 tatagaaagg acattagtat aatctcctcc tcaatatatt atcattttgg ttggtttgat
    17881 agagctctct aattgtcctc tttgagcttt ctctccttgt gatgtttgtt caaggatagt
    17941 tggtgaagta atgagatatt ttcaagggag agcaattata tcagagtggg aactgtgcta
    18001 gggctaggga gtgattgctt acacttcatg atatatttca gaattaaaag aaaacagaaa
    18061 aatctgtact attttgtata gttaagatta tcttcctgat aaaagaataa tttcatgtca
    18121 aagagaaata atgttttctc tgtagattga tttcatatgt ggagggagag ggaatccata
    18181 ttccattcat actactatga ccttttgcct ttaggttaat ttttctaata tttagaaaaa
    18241 aattaaaatt ttaatgtaaa atattcaatt ttgttaacag accctaaaca atgttttcga
    18301 tgaatgctat actcctttaa aatgtgaaat gaatatagaa gagaaagaga attctggtgt
    18361 ttacacttct caaaatcaaa gcccagaatt acaagagtat agatacgaag ttcaaggtaa
    18421 aaagcttact atactgttaa acaggattta ctttgctcaa gtcaattgct tataaacgta
    18481 actttaagca acttaatgat agatacgttt tcttagtatc ttatttttag ccatgaaatt
    18541 catatttctt tctccttgat ttcttcattc caatctattt tttattctaa ttctttttgt
    18601 taactgtgct aactgtagat cttaatcttt aaaatttaga gtttactaag tttaaattaa
    18661 tggtttataa ttttaaaagt ggattaagaa attgagtttt aagatacatt aagaaatgac
    18721 ctctgccagc tgtggtagtg catgtatgca ggcctttaat ccccacgctc aggtggcaga
    18781 ggcagaggca tctgtgtaag tccttggccc gctgagtctg caccgctgtt tgaggctgac
    18841 cagggcaggg ctaaatatag aaagagataa ttttcttcat gtaaatgtgg tgctagtttt
    18901 gaatttattg gtgttcctta acagatttat tatgttccta gtatatttgt ataggtaacg
    18961 agcaacctgc taataaacag ctacagaaac taatacatct taagataaaa ttttcaagat
    19021 aatgtcatac cagttacttc atagtaaagt tctaagtagg taaatcttgt tttgtaaaag
    19081 aggtaaactt gtacagtgaa atgaaatgat tatcatggtc tgttcttcct tcagactttc
    19141 aagagagcat gcaagtcctt cttggtaaag tgacggaaga atgccgcaaa ctctcaggac
    19201 tgcagacgcg cttaggcaag gtctgtggga tagaggagat gatatctgca tttgttttac
    19261 tgcctgctgt tgtgtcaatc aggaagttct aaaattggtg aacgtggaga aggatgctag
    19321 aaatattaaa tcatttatat gtttgtatta gaacatctat atcttaagct gaagaaactt
    19381 ccagaattta aatggtatgt aacttgctga gacgagtttt taagtcatga tgaagtgtaa
    19441 gtggatgtca aatgttaaag tatgaaacaa ttgtatttag ttgtatagat gtctttcctt
    19501 tttagcttac tggagcttaa acacacagta gaacagtaac ctatcactaa actacacctg
    19561 taggctctta catgtagtat tttaaggcaa tgcgtctgtc tttatatgtt aaagacgata
    19621 attagttgtt ttgcacatca ctgatatcac atttaacatt taactctgtc tgtgtacttt
    19681 tgttgatgtg ccctctgatg ggccacgttt ctctgcccca ctgccttctt tgcgggtgct
    19741 ggctttttct gtggttcagc cagcaccaac cgttggctct gccccagccc ttcagtccaa
    19801 tactggctac agcattgtgt ctgatcttac tagagtcact tgacacactt caatttttcc
    19861 tgtatgagtg gaagatcatg ctcagtttgt gtgccaatgg cattcacctg agcgtaggag
    19921 tccctggttt tctatcaagt gataggcaac ttccacaagt gttcacacag accatagtgc
    19981 ttttctgtag caattgtaac ccacaatgac cattcaggca ggtctatat
//