LOCUS       KO-first_condition_ready_41212_MGI:2143315        22177 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Klhl18 targeting project(s): 41212
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Pgk_PM
COMMENT     design_id: 42066
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7848..8008
                     /db_xref="ENSEMBL:ENSMUSE00001249190"
                     /rank="5"
                     /note="ENSMUSE00001249190"
     misc_feature    4275..8989
                     /note="5 arm"
     misc_feature    9017..9041
                     /note="B1 Gateway"
     misc_feature    9054..9101
                     /note="Frt"
     intron          9102..9976
                     /note="mouse En2 intron"
     misc_feature    9974..9976
                     /note="Splice Acceptor"
     exon            9977..9978
                     /note="mouse En2 exon"
     misc_feature    10190..10751
                     /note="ECMV IRES"
     gene            10762..13808
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13843..14098
                     /note="SV40 polyadenylation site"
     misc_feature    14102..14135
                     /note="loxP"
     promoter        14167..14674
                     /note="mouse phosphoglycerate kinase 1 promoter"
     gene            14761..15552
                     /gene="NeoR*"
                     /note="NeoR*"
     misc_feature    15750..15993
                     /note="SV40 polyadenylation site"
     misc_feature    16010..16057
                     /note="Frt"
     misc_feature    16064..16097
                     /note="loxP"
     misc_feature    9991..10068
                     /note="FCHK std_gpr"
     misc_feature    16113..16133
                     /note="B2 Gateway"
     misc_feature    8990..16163
                     /note="Synthetic Cassette"
     exon            16523..16659
                     /db_xref="ENSEMBL:ENSMUSE00001219951"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 1 ENSMUSE00001219951"
     misc_feature    16164..16863
                     /note="Critical Region"
     misc_feature    16887..16920
                     /note="Downstream LoxP"
     misc_feature    16864..16943
                     /note="synthetic loxP region"
     exon            21528..21632
                     /db_xref="ENSEMBL:ENSMUSE00001212528"
                     /rank="8"
                     /note="ENSMUSE00001212528"
     exon            17833..18055
                     /db_xref="ENSEMBL:ENSMUSE00001268000"
                     /rank="7"
                     /note="ENSMUSE00001268000"
     misc_feature    16944..22177
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatattga catttttctc tggtttgttt ctgtcttctc agttgagagg
     4321 gagcctgagg ggagaatatt agaaatctgg gggaatgaaa tatatgagat ggacagatga
     4381 gcctgggaga ttgagaaggg cagagagatg gagaggggca gggagatggg gctgatgatt
     4441 tggcttaaac gtaaagtgct cagctccatg ggtgttctct gtcagtgtcg catattggtg
     4501 caggtattga ataaaaggca cactgaactt gaagagctgg actttaaagg ctggagaggt
     4561 ggtccagtgg ttaagcgtat ctgctgttct tccaggggac ccatgtctga tttcctcatg
     4621 ttgcagggct taaaaccacc tatagctcca ggaaatcagt caccctatct gtcctatggg
     4681 caccctacac acatgtgaca cacacactca cacacactca catacacaca cattaattat
     4741 ttaaaataaa tctttaaaaa tatgcggcct ttatgaaatt agcagtggtg gtggtgtttt
     4801 gttttgtttt gttttttcta gagtgagcat gtccaaagga gggaggagct tggcacagga
     4861 atggaggaca cccatgtagc catctctaga acacaggtca gcagagaggt gaggaactag
     4921 aacagatcta gagagctgac agggaggagg accaggcaat gtgagaggca atcagggatt
     4981 tctgagctgg aagaggtgga agtggtgttg aagtatgggt ggacttgaaa ctctggaatc
     5041 agggtcaggc atgggatcta cttggagaga gcttgcctac catgaacaaa gccctcgatc
     5101 cagttcctag taccacataa agaagacatg gctgtgcaca tttgtaatcc cagcactgga
     5161 gacgaaggca gcagacttac aagtttatgc ttatctttaa ttgtataaca agttgggact
     5221 gggcataggc acaaatcttt catcttagca tgtgggaggc agagacagga gaatctctat
     5281 gagtttaaga ccagcctgtt ccacatagca agttccaggt cagctacaaa gcaagttcca
     5341 gactagttaa gtagtgagac tctatctcaa aaacaagttg gaaactagcc tgggaaacac
     5401 acacacacac acacacacac acacacacac acgctcgcac acacaaacac atacacctcg
     5461 ctatgaaatt gggaagttct tgaaatctgt ttataacaac cattttgtat ggtggcacta
     5521 ttggtaaagc aaagtaaaag tcttaccttc ttggagtggt caagaaaaat taaaataaaa
     5581 gcagattgtt atcaggcagg gcaaggaaga gcaaaggatg cacctatcag cagaggtgta
     5641 gtgtaagtag ccttaggaag acagtgtgca gcaaccacac ggtggctcac agccatctga
     5701 aaacagctac agtgtactta catataataa ataaataaat aaataaataa ataaataaat
     5761 aaataaattt ttttaaaaaa ggaagctagt gtgtgatgtt tgggacagtg ttgagggcta
     5821 gcagtgaagg acattgcatg cacggtaaca agtttggttc agcaaactcc tggggtcatc
     5881 actattgcct ttgatccact gagaagtcca ataaaaatgt aggatgtttg tagccaagag
     5941 ccgtagcctg gttgagtggc tgaagagttt gttcttctag acgggggacc tgaattggaa
     6001 tccgtagagc ctacattaag ctaggcacag cagtgacctc cggtgggtcc cagtactctt
     6061 tttgtaaaag gggaagcaaa gacagaaagg ttccaggaag cctgtggtcc aactagtctg
     6121 ctgtacacag ttagcaaaca acaagatggg agaggagggt tgtcacccaa ggttgtcctc
     6181 tgacctctat ccatgtggtg ctgtggcaag cgtgtgccgg gtgcatacaa tgggggaggg
     6241 gaggagttac tgctgtgaat agtgcctggt ggggaggact gcggatgaga aaataaagaa
     6301 ggcagaaacc tgggattgag gttttgcata aactgatagc ttatgtcaag caagtgtatt
     6361 aaatagtata gcattctgcc gggcagtggt ggcgcacgtc tttaatctca gcactcagga
     6421 ggcagaggct ggcagatctc tgagctcaag gccagcctgg actacagagt gagttccaag
     6481 acagccaggg ctgtactgag aaaccctgtc tcaaacaaac aaaagaagaa tagcatcttg
     6541 catattgttt acagcggggg gggggggggg gggcgaatgg gccacggtca gcaggaagct
     6601 aagtcagaca atgtggcaag agaacacagg atacctcaga cacagctgcc tcaggactga
     6661 taactgcagt ctttcatggg gaaaaggcga tttccccagg aaccaaaacc ttaatatgtg
     6721 agcctggccc cagccccata ctagaccttg ccaagtctgc ccctgggcaa ggacagagct
     6781 agagttctct ttgtcctttc attagggctg tgagaaatct tggattcatc taactcccaa
     6841 cactaggagg cagccgtcga gatgctaaca caaactcagc taaattccgt aactatctga
     6901 agataacctg ccccaccctt aactattagt gacaggtaga gaagtggccc ctgagcttag
     6961 cagcatggac agagacttag aagcacggga tcctaagaac agaatgttta cctcctaggt
     7021 tcttccagta accaatgtat cctggagcag cccacgcgag tttatttgag tagcaatctg
     7081 cgaggcctga ggggcttcaa ctggttatat tcttgtgttt gaaggaataa ggaaggttgc
     7141 taggtggaca gtgtaaacag aggaactggc ctggcctgga atttagagct ggttggatgc
     7201 aagatcaacc aggaaaaaga tgttctgttg tgatgcggaa aagaatcgat aatgaaatcc
     7261 aggaagcttt ccagtaaccc aggcttacat tacagaccag tggacctaag ctttccgtgg
     7321 tctcgtgtgc ctggaactct agcacttggg agcatcttga atccttttag gccagcctgg
     7381 gctgcatcgt aaactgtctc aaaaacaaaa gactaggcct aagagcatag ctggaggagt
     7441 gtaaagaaat ctgtggatgg gctggagaga tggctcagcg gttaagggca tcaactgctc
     7501 ttccagaggt cccgagttcg aatcctagca accacaaggt ggctcacaac catctctcat
     7561 gaaatctgat gccctctctg gtgtgtctaa aaacagagat gtactatcac tagataggta
     7621 ggtaggtagg taggtaggta ggtaggtagg taggtaggta ggtaggtaga tagatagatc
     7681 ttaaaaaaaa caaaaacaaa agaaagcagt ggattgcaga catgggtggg aggaaagctg
     7741 gatcgcctga ggacctgcct cagtacaggc agtggaagaa ctgagagcga ggaggcagga
     7801 gcgtggtctc tgggtagctg caagagcatc tgctctttgt cccataggtt tttgaagctg
     7861 cattggcctg ggtcaggtat gaccgggagc agaggggacc atgcctgccc gagctgctgt
     7921 ccaatatccg cctgcctctt tgccggcccc agttcttatc agatcgagtg cagcaagatg
     7981 acctggtacg gtgctgtcac aaatgcaggt aagtgtgggt ggccttgtcc aggacacggc
     8041 ccagcaaaga ggaggaattg gccacctgtc gtagctcagg ggaggaagga tttggagcag
     8101 gcagttaact cacacattgc tcacaagcag agagatgcca tgtaggagca gaaggggctt
     8161 ttcttcccta agttatctat gtgttcttca gcttcatcca ctgagagtta tgaatgtact
     8221 ctgtgtatgc tgcccacact ggcagctgac ttgatagtac aaatggggct tcccggaggc
     8281 tccctgccct ctgcaataaa ttgtagcttg tgactagcct ctgatgagtg atgggggggg
     8341 gggcttgtat accattgtgt agagcttctg cctagagcca ccagcacagg agatgggttg
     8401 ttaagcaatt taaacaaaga aggcctgtct tcccagttgc ttgttatagc cacaatgctg
     8461 ggttttggtt ttttccttta ctgacaagat ttttacacat tgagtttccc atgattccac
     8521 ccctagttgg gatcagcagg ttccgggagt aagcagtggc gtggtacagg gctttgtgat
     8581 agtatgtcag ggagcaagaa ctgtgtggct ggagggagcc ggggccctgt gacaagcagt
     8641 ctgaaggata atatcgaggc atacgaacta ttctgtcagc agtaggtagc taacaaaaga
     8701 aggttcttag agaaaagcaa tgtagtctgt ggtttggagg ggctggggag ggcaagccag
     8761 ggcctgagaa atccatctgg agcacttgac agcagttgcc gaggtgcaag caaggggtga
     8821 ttgacaggag ggtgtgagcc gtggatggtg atgggcagga aggaagagct aggtctgagt
     8881 gctgatagga agtaggctgc ttgagttccc ccaatgctcc cattttaact cccacactgt
     8941 ttttcaaaat cacatttggt ttgtgcctgt taggaggagt tgccccggta aggcgcataa
     9001 cgataccacg atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc
     9061 ctattccgaa gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac
     9121 cctccacact tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga
     9181 agaacccgtt gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt
     9241 gggtggcctg cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg
     9301 ccaccaaggt cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga
     9361 agactgaagg ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc
     9421 aaggaaacaa acttggcctc accaggcctc agccttggct cctcctggga actctactgc
     9481 ccttgggatc cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact
     9541 ggctagccta ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt
     9601 ccctaggata gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag
     9661 attttggctg ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc
     9721 ttttagctac atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg
     9781 gtcagtgggc acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt
     9841 atggccttgg gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt
     9901 gggtgcgttg gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct
     9961 cctccatgac aaccaggtcc caggtcccga aaaccaaaga agaagaaccc taacaaagag
    10021 gacaagcggc ctcgcacagc cttcactgct gagcagctcc agaggctcaa ggctgagttt
    10081 cagaccaaca ggtacctgac agagcagcgg cgccagagtc tggcacagga gctcggtacc
    10141 cggaagatct ggactctaga gaattccgcc cctctccctc ccccccccct aacgttactg
    10201 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
    10261 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
    10321 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
    10381 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
    10441 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
    10501 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
    10561 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
    10621 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
    10681 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
    10741 aagcttgcca caaccatgga agatcccgtc gttttacaac gtcgtgactg ggaaaaccct
    10801 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    10861 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
    10921 tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct
    10981 gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc
    11041 tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg
    11101 acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg
    11161 cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc
    11221 ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc
    11281 ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat
    11341 caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact
    11401 acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta
    11461 ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct
    11521 ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc
    11581 gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa
    11641 ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac
    11701 ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat
    11761 ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat
    11821 catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg
    11881 aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac
    11941 acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc
    12001 atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc
    12061 gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg
    12121 aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat
    12181 ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt
    12241 tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc
    12301 atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc
    12361 cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat
    12421 ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat
    12481 gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc
    12541 cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa
    12601 gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc
    12661 agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat
    12721 ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg
    12781 attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc
    12841 gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag
    12901 tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat
    12961 ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac
    13021 cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg
    13081 ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc
    13141 cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa
    13201 gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct
    13261 cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat
    13321 ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg
    13381 gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga
    13441 ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat
    13501 ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc
    13561 gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc
    13621 agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa
    13681 gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg
    13741 agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc
    13801 tggtgtcaaa aataataata accgggcagg ggggatctaa gctctagata agtaatgatc
    13861 ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    13921 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    13981 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    14041 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgg
    14101 aataacttcg tatagcatac attatacgaa gttatgtttc tagcttggct gcaggtcgtc
    14161 gaaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
    14221 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
    14281 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
    14341 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
    14401 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
    14461 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
    14521 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
    14581 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
    14641 gttctcctct tcctcatctc cgggcctttc gacctgcagc ctgttgacaa ttaatcatcg
    14701 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat gggatcggcc
    14761 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
    14821 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
    14881 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
    14941 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
    15001 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
    15061 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
    15121 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
    15181 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
    15241 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
    15301 gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
    15361 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
    15421 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
    15481 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
    15541 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
    15601 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
    15661 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
    15721 accccccgga tctaagctct agataagtaa tgatcataat cagccatatc acatctgtag
    15781 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    15841 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    15901 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    15961 aactcatcaa tgtatcttat catgtctgga tccgggggta ccgcgtcgag aagttcctat
    16021 tccgaagttc ctattctcta gaaagtatag gaacttcgtc gagataactt cgtatagcat
    16081 acattatacg aagttatgtc gagatatcta gacccagctt tcttgtacaa agtggttgat
    16141 atctctatag tcgcagtagg cggagttcct gacactaaac ctcactgggg atagaactca
    16201 gtagtagagc attcaggctt ctctgtggcc ctagattcac tccctagcat gagagacaga
    16261 cagagagaca gagacacaca gagacagaga cagagagaca gagagagaga cagaggcaga
    16321 gagagagaga cagagagacg gagagagaga gagagaccaa aagagacagg tggacagaca
    16381 gacagacact ggtagaccaa attttccaca agacttttcc tagattccct acactgagat
    16441 ctggaggagc caggcccata agtgtaaagg tagaccaaaa tgagaggtgc ctcctctcct
    16501 agcatcgtgc gccttattcc agggacctgg tcgatgaagc aaaggactat catctgatgc
    16561 cagagcgccg gccccacctg ccagctttca ggactcggcc ccgatgctgc acgtccatcg
    16621 ctgggctcat ctacgctgtg gggggcctca actcagcagg tatcttgcag ctcccctttg
    16681 caaagccctt cctagagtga atgaagaaac ccagtcaagc tagcctggtt gttctggttc
    16741 ctcctcttta tgctgttctg tctcccgtcc ttatcttaag acaccagaga gtagccctag
    16801 agtagggctt attatggatt cataatattg aattgatgtc aatgctaaag gtgccttgaa
    16861 gaagagatgg cgcaacgcaa ttaatgataa cttcgtatag catacattat acgaagttat
    16921 ggtctgagct cgccatcagt tcaatgctga aacatcagca gctccattac acacggtatt
    16981 acccagtact tcagagccta aagcaggagg gtctggagtt tgaggccagc ctgggctaca
    17041 gttattgaga ctctgcttcc aaaaacaaaa cagtacaaaa ccaccattac ctatagctct
    17101 tgtactatag acagtcacat ttctaggaaa gcctcctggg gggtcagggg gaaattgaca
    17161 gatctgtttt cagttaaggt taggaaaggt agctagtgac cttctgcttg ttaacagcat
    17221 aacctagaaa agataagtct tcagaaacaa acactggatg taacaagggc ttggtccacg
    17281 caagccacag ggaagataat tgtctgagtt gctaatcatg atctggggag gatgagaggg
    17341 ttcctgggtt ctgtggctgc tctttactga gttctaagta tgttctagat ccatgcaggg
    17401 tgctgtatcc attatgtcat ttaaccctca tggcagttct ggtagccttt aacttcacat
    17461 ccaaaacagc atgaggaccc aatgcagccc gtctggtcat tagatgaagt ggccggtagc
    17521 actgggaccc atgcacttga ctctggcatg ctttctgcat tgggcagacc atcctgtgca
    17581 tttgccctca ttgtcgattt caagcttacc tgattatgac tgagaactac tctcattaaa
    17641 gagcaaatgt ttcccatctg agtctaaaat ctttattata gctagagttt ttagtgggcc
    17701 cacttactct gagtaatttg tgggaagagc caataggatt gagtcagatg ttccgtatag
    17761 tcaataccca gtagctgtga gctaaacaaa tggtctcatt tgttttaatt tgagtagcaa
    17821 atttttatgc aggtgattcc ctgaatgtgg tggaagtgtt cgaccctatc gccaatcgct
    17881 gggaaaagtg ccatcccatg acaacagccc gaagccgtgt gggtgtggct gtggtgaacg
    17941 ggctcctcta tgctatcggg ggatatgatg gtcagttgcg gctgagcacc gtggaggcct
    18001 acaatcctga gacggacaca tggacccgag tggggagcat gaatagcaag cgaaggtatt
    18061 ctggaagcca agtgcaggct gagcacacac tgagcatctg gggagcccca tgtaggggca
    18121 cataataaag taaatgcctg ggaagtcctg ctgccaggcc agccactgcc agcacctcct
    18181 gggagcaagc ccccatctgc ttaagcataa aatacctgcc ttgtcctttg aggaacttgg
    18241 ggagccaaga catggacatg ttatgtaagt ggcagctaag gactgaaaat gtgtgtaatg
    18301 gaagattcca ggagggcgag agactagact ggaagatctg ggaagaggtg ggattaaagt
    18361 tgaagcctga aggataagga gttttaacct cattagtaat ggaggaagcc cggggtggtt
    18421 ttctctgtgc ttgctttctc gtgccataca tacgctcccg ctgccccgga agcgcacact
    18481 ctccagacgt gagcacgcgt gtattcacga ggtggactga acttgtacat catccaacat
    18541 ggtctagcta tccgtgtctc ccaccgctcg cctgctcatt tccaggatga gaagtatggc
    18601 tggaggacac cgtggtagtg cccgagtgta accctcttag tccagagatg atgggcagtg
    18661 tccttcctta cgtcaggcat gcgctgcagt ggtaggggcc cacactattt aaagggtgtc
    18721 aatcagtcag ccacagcaga cgagcagtca caaatgttac tcttctggct ctcctcatag
    18781 ggtctgagaa ggaaggaagg aagcccaagg acccttgcac atttaaccag aacaggtctg
    18841 cctgtatgtt gagtttctat gacttgtact caattttagg gaggagaata atgtgctgtt
    18901 tttaaaggat actttgaaac ctaccagcca cttgctcttt aaaggtattt ctgtatagtt
    18961 ctctggccta gccttggcct cacaggccca tcctccttgc catacatctg gttctctact
    19021 tgggagatca catgtctggg aagggggaaa agatgccaag tgatggcacg tgtggtgagg
    19081 cagtggttag cgtggagggc tttgacgccc agaggcgatc cacgttgagc agtactggcc
    19141 tgagccggaa ggcacctgct cagcagcagg gagggcagag ttacagagtg cacagaggtc
    19201 tgtgcgcatc cctgtaggga gaagcagtgt atgcgcacga tgtgcttctt ccaaggacgt
    19261 tcccagagtt ctctcttccc ctccccacct ttctgtgtct accctctgcc tttgtacagt
    19321 ttgcagcttt ttgtgaatga gggacccctt tctcatatcc cagccacagg tggctctttt
    19381 actttgacag cgtgtgtgtc cctctaggag acgttctggc tttgatgtcg gagggtttgt
    19441 tgtgttattt aaatgggagg aaagttctct gggcatccct ctccttcgtg tggctaatgc
    19501 cctggtatta gcaatatggg cccagttcca tttgtccctt ttaaattaca ggttccctta
    19561 aatgttctta gtcacttctg ttctgacatc ccgcatagct ctccatcctg ccagtgacag
    19621 cttcctttct gctcgctttc catctttgtt tctggtactg gattgttcca gcccatccct
    19681 cttacttgac tgttgtctat ggtttgggag cacctcttga ttcattctcc catcattgct
    19741 cacctcctga cttagccgcc ttgtgcttag gaccaaggca gcagctcagt ggtaggaagc
    19801 ttgctctaac aagagcgagg cccgaggttc aattcccaat gttatatacc aagaaaaagg
    19861 aaatagagtt acttcaggta tgtttcacat ccatgtctag gagggggatc aatgcctatt
    19921 aaatttgtca ttttcctaat gatcttcctt aagacaagga agatctcctc ttagagcagg
    19981 ttcaaagggc tgttactgta gtttgacttg ctatgtatac ttgaaaagtg gcagtttcca
    20041 gtttttagtc tctgtagcat caacactttg tcaggcagct tggcattacc gcttaaagtc
    20101 tcacaaggtt tatatagggt tgaaggttat ctgtgcttac ctagaccctg ggtaatcagg
    20161 attttaggga agcttatgtg tggaacaggg gactctgggc ttgtgttgtt tagctctcag
    20221 gcatgtcttc tccaagcagc cttgagaaga gcccagcagg ccatgcttac agcaattctt
    20281 ccttgaaggg tgatgcataa gtgtataaca aacagaagct ggaatctcct ccaccaaggc
    20341 caaggagggg ctgtgcagag ccaggaatca cagaagcaca ggctaaacta caaaggaact
    20401 gagtaggaag tcagaggcaa cacacatact acctcatctc atggtgacac atgagtaccc
    20461 atcatcagga gtttacctca tctcgtggtg acacatgagt acccatcatc aggagttttg
    20521 gctgttcatt tgttactttt gcttgtttga ttgattgatt tttttttttc ttaaaaaaaa
    20581 aaaggctctt gctatgtagc ccaggctgct ctctaacttg agccctccct gcctcagcct
    20641 cctcagccct gggattgcac caccatgccg agcctttgtt ctccactcat aaaaccattg
    20701 tctaaaacat tatgccttct acatggctga tgaaactctg gtgaatgaat agatgtgaca
    20761 tgtgtcctaa tagagaagcc actcttggag catggggtca gaaggtagta attctgaaag
    20821 tgtgtgactg aacccacagt taaattaggc tgaggccctg ggtttgctag gtgggccaaa
    20881 gtgcttacta tacagacctg atgaatttgt gcttagtcct aagaacccac agtggaagga
    20941 gagacccagc tcccagatgc tgtcctctga tctctgcaca tgtgcacacg catacacaca
    21001 cacattaata ataatacatt tttattcttt cttgttattt atatctgtgt tggagtgttt
    21061 tgcttttatg tatatctgta tagcacatgc ctgtagttct ttggaagagc agccagtact
    21121 caacctctga gccatctctc cagcctaaaa gtagtttggg gtaaatcagg ctagtcacga
    21181 cggtgtcacc tataatctca gcagttggaa aatggaggca gggggattag gggtgcaggg
    21241 ccatcctcag ctacataaac aagtgaatga actcatcctg ccatgggatg agaggtgagc
    21301 acttcaggca gatcaagggc tgaatgcctg aggaggagag agctcgttgg gggggggggg
    21361 gggatcgggg aggaagcagg agcagaggac cactgaacag atctttgtgg tctgccctta
    21421 cctggttgtg tccctgggat cgggtctgtt ctagaaaaag acagttgaca gattagtttc
    21481 ttagcgtgtg tgagaggctg ctgggatgac cattctgtct gtttcagtgc catggggaca
    21541 gtcgtgctgg atggacagat ctacgtgtgt ggaggctatg acggcaactc ctccctcaac
    21601 tctgtggaga cctactcacc tgagacggac aagtaaggct gacttatccc cctgcctcag
    21661 cttcttgagt gctgaaatta cagatagaaa ctattatact cggcctacct tccccacctc
    21721 caggcctttg tgcttgccag gcttccagcc cccctccccc cacccccact cccacctctt
    21781 gtttagtttt gctttgcttt gttggagaaa aggtctcaac ctcaggactc tgatgagcca
    21841 cctcagccac agagacatgc cgtggcacgg agcttaaagc ttctcctggc taggaagtga
    21901 cttaacaata cttgcatgta gtcgatgtct acatccttgg ctaaatgtca gacacggtga
    21961 tgcgcccctt tgatcccttt acttgaaggc agaggcaagt gtgtctccgt gagttcaaaa
    22021 ccagtctgct ttatgtagtg cattctagac tagccaaggc tacaaagcag gacttggtct
    22081 caaaaaaaca aaacaaaaaa atatattttt taattccttg tttaagtgaa atttcatggt
    22141 taaatttcaa aagagctaaa gcaatggtgg ttcaatc
//