LOCUS       KO-first_condition_ready_41214_MGI:2143315        22125 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Klhl18 targeting project(s): 41214
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 42066
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            7848..8008
                     /db_xref="ENSEMBL:ENSMUSE00001249190"
                     /rank="5"
                     /note="ENSMUSE00001249190"
     misc_feature    4275..8989
                     /note="5 arm"
     misc_feature    9017..9041
                     /note="B1 Gateway"
     misc_feature    9054..9101
                     /note="Frt"
     intron          9102..9976
                     /note="mouse En2 intron"
     misc_feature    9974..9976
                     /note="Splice Acceptor"
     exon            9977..10149
                     /note="mouse En2 exon"
     misc_feature    10161..10754
                     /note="ECMV IRES"
     gene            10755..13815
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13854..14097
                     /note="SV40 polyadenylation site"
     misc_feature    14101..14134
                     /note="loxP"
     promoter        14161..14680
                     /note="human beta actin promoter"
     gene            14706..15500
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15700..15941
                     /note="SV40 polyadenylation site"
     misc_feature    15958..16005
                     /note="Frt"
     misc_feature    16012..16045
                     /note="loxP"
     misc_feature    9991..10068
                     /note="FCHK std_gpr"
     misc_feature    16061..16081
                     /note="B2 Gateway"
     misc_feature    8990..16111
                     /note="Synthetic Cassette"
     exon            16471..16607
                     /db_xref="ENSEMBL:ENSMUSE00001219951"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 1 ENSMUSE00001219951"
     misc_feature    16112..16811
                     /note="Critical Region"
     misc_feature    16835..16868
                     /note="Downstream LoxP"
     misc_feature    16812..16891
                     /note="synthetic loxP region"
     exon            21476..21580
                     /db_xref="ENSEMBL:ENSMUSE00001212528"
                     /rank="8"
                     /note="ENSMUSE00001212528"
     exon            17781..18003
                     /db_xref="ENSEMBL:ENSMUSE00001268000"
                     /rank="7"
                     /note="ENSMUSE00001268000"
     misc_feature    16892..22125
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatattga catttttctc tggtttgttt ctgtcttctc agttgagagg
     4321 gagcctgagg ggagaatatt agaaatctgg gggaatgaaa tatatgagat ggacagatga
     4381 gcctgggaga ttgagaaggg cagagagatg gagaggggca gggagatggg gctgatgatt
     4441 tggcttaaac gtaaagtgct cagctccatg ggtgttctct gtcagtgtcg catattggtg
     4501 caggtattga ataaaaggca cactgaactt gaagagctgg actttaaagg ctggagaggt
     4561 ggtccagtgg ttaagcgtat ctgctgttct tccaggggac ccatgtctga tttcctcatg
     4621 ttgcagggct taaaaccacc tatagctcca ggaaatcagt caccctatct gtcctatggg
     4681 caccctacac acatgtgaca cacacactca cacacactca catacacaca cattaattat
     4741 ttaaaataaa tctttaaaaa tatgcggcct ttatgaaatt agcagtggtg gtggtgtttt
     4801 gttttgtttt gttttttcta gagtgagcat gtccaaagga gggaggagct tggcacagga
     4861 atggaggaca cccatgtagc catctctaga acacaggtca gcagagaggt gaggaactag
     4921 aacagatcta gagagctgac agggaggagg accaggcaat gtgagaggca atcagggatt
     4981 tctgagctgg aagaggtgga agtggtgttg aagtatgggt ggacttgaaa ctctggaatc
     5041 agggtcaggc atgggatcta cttggagaga gcttgcctac catgaacaaa gccctcgatc
     5101 cagttcctag taccacataa agaagacatg gctgtgcaca tttgtaatcc cagcactgga
     5161 gacgaaggca gcagacttac aagtttatgc ttatctttaa ttgtataaca agttgggact
     5221 gggcataggc acaaatcttt catcttagca tgtgggaggc agagacagga gaatctctat
     5281 gagtttaaga ccagcctgtt ccacatagca agttccaggt cagctacaaa gcaagttcca
     5341 gactagttaa gtagtgagac tctatctcaa aaacaagttg gaaactagcc tgggaaacac
     5401 acacacacac acacacacac acacacacac acgctcgcac acacaaacac atacacctcg
     5461 ctatgaaatt gggaagttct tgaaatctgt ttataacaac cattttgtat ggtggcacta
     5521 ttggtaaagc aaagtaaaag tcttaccttc ttggagtggt caagaaaaat taaaataaaa
     5581 gcagattgtt atcaggcagg gcaaggaaga gcaaaggatg cacctatcag cagaggtgta
     5641 gtgtaagtag ccttaggaag acagtgtgca gcaaccacac ggtggctcac agccatctga
     5701 aaacagctac agtgtactta catataataa ataaataaat aaataaataa ataaataaat
     5761 aaataaattt ttttaaaaaa ggaagctagt gtgtgatgtt tgggacagtg ttgagggcta
     5821 gcagtgaagg acattgcatg cacggtaaca agtttggttc agcaaactcc tggggtcatc
     5881 actattgcct ttgatccact gagaagtcca ataaaaatgt aggatgtttg tagccaagag
     5941 ccgtagcctg gttgagtggc tgaagagttt gttcttctag acgggggacc tgaattggaa
     6001 tccgtagagc ctacattaag ctaggcacag cagtgacctc cggtgggtcc cagtactctt
     6061 tttgtaaaag gggaagcaaa gacagaaagg ttccaggaag cctgtggtcc aactagtctg
     6121 ctgtacacag ttagcaaaca acaagatggg agaggagggt tgtcacccaa ggttgtcctc
     6181 tgacctctat ccatgtggtg ctgtggcaag cgtgtgccgg gtgcatacaa tgggggaggg
     6241 gaggagttac tgctgtgaat agtgcctggt ggggaggact gcggatgaga aaataaagaa
     6301 ggcagaaacc tgggattgag gttttgcata aactgatagc ttatgtcaag caagtgtatt
     6361 aaatagtata gcattctgcc gggcagtggt ggcgcacgtc tttaatctca gcactcagga
     6421 ggcagaggct ggcagatctc tgagctcaag gccagcctgg actacagagt gagttccaag
     6481 acagccaggg ctgtactgag aaaccctgtc tcaaacaaac aaaagaagaa tagcatcttg
     6541 catattgttt acagcggggg gggggggggg gggcgaatgg gccacggtca gcaggaagct
     6601 aagtcagaca atgtggcaag agaacacagg atacctcaga cacagctgcc tcaggactga
     6661 taactgcagt ctttcatggg gaaaaggcga tttccccagg aaccaaaacc ttaatatgtg
     6721 agcctggccc cagccccata ctagaccttg ccaagtctgc ccctgggcaa ggacagagct
     6781 agagttctct ttgtcctttc attagggctg tgagaaatct tggattcatc taactcccaa
     6841 cactaggagg cagccgtcga gatgctaaca caaactcagc taaattccgt aactatctga
     6901 agataacctg ccccaccctt aactattagt gacaggtaga gaagtggccc ctgagcttag
     6961 cagcatggac agagacttag aagcacggga tcctaagaac agaatgttta cctcctaggt
     7021 tcttccagta accaatgtat cctggagcag cccacgcgag tttatttgag tagcaatctg
     7081 cgaggcctga ggggcttcaa ctggttatat tcttgtgttt gaaggaataa ggaaggttgc
     7141 taggtggaca gtgtaaacag aggaactggc ctggcctgga atttagagct ggttggatgc
     7201 aagatcaacc aggaaaaaga tgttctgttg tgatgcggaa aagaatcgat aatgaaatcc
     7261 aggaagcttt ccagtaaccc aggcttacat tacagaccag tggacctaag ctttccgtgg
     7321 tctcgtgtgc ctggaactct agcacttggg agcatcttga atccttttag gccagcctgg
     7381 gctgcatcgt aaactgtctc aaaaacaaaa gactaggcct aagagcatag ctggaggagt
     7441 gtaaagaaat ctgtggatgg gctggagaga tggctcagcg gttaagggca tcaactgctc
     7501 ttccagaggt cccgagttcg aatcctagca accacaaggt ggctcacaac catctctcat
     7561 gaaatctgat gccctctctg gtgtgtctaa aaacagagat gtactatcac tagataggta
     7621 ggtaggtagg taggtaggta ggtaggtagg taggtaggta ggtaggtaga tagatagatc
     7681 ttaaaaaaaa caaaaacaaa agaaagcagt ggattgcaga catgggtggg aggaaagctg
     7741 gatcgcctga ggacctgcct cagtacaggc agtggaagaa ctgagagcga ggaggcagga
     7801 gcgtggtctc tgggtagctg caagagcatc tgctctttgt cccataggtt tttgaagctg
     7861 cattggcctg ggtcaggtat gaccgggagc agaggggacc atgcctgccc gagctgctgt
     7921 ccaatatccg cctgcctctt tgccggcccc agttcttatc agatcgagtg cagcaagatg
     7981 acctggtacg gtgctgtcac aaatgcaggt aagtgtgggt ggccttgtcc aggacacggc
     8041 ccagcaaaga ggaggaattg gccacctgtc gtagctcagg ggaggaagga tttggagcag
     8101 gcagttaact cacacattgc tcacaagcag agagatgcca tgtaggagca gaaggggctt
     8161 ttcttcccta agttatctat gtgttcttca gcttcatcca ctgagagtta tgaatgtact
     8221 ctgtgtatgc tgcccacact ggcagctgac ttgatagtac aaatggggct tcccggaggc
     8281 tccctgccct ctgcaataaa ttgtagcttg tgactagcct ctgatgagtg atgggggggg
     8341 gggcttgtat accattgtgt agagcttctg cctagagcca ccagcacagg agatgggttg
     8401 ttaagcaatt taaacaaaga aggcctgtct tcccagttgc ttgttatagc cacaatgctg
     8461 ggttttggtt ttttccttta ctgacaagat ttttacacat tgagtttccc atgattccac
     8521 ccctagttgg gatcagcagg ttccgggagt aagcagtggc gtggtacagg gctttgtgat
     8581 agtatgtcag ggagcaagaa ctgtgtggct ggagggagcc ggggccctgt gacaagcagt
     8641 ctgaaggata atatcgaggc atacgaacta ttctgtcagc agtaggtagc taacaaaaga
     8701 aggttcttag agaaaagcaa tgtagtctgt ggtttggagg ggctggggag ggcaagccag
     8761 ggcctgagaa atccatctgg agcacttgac agcagttgcc gaggtgcaag caaggggtga
     8821 ttgacaggag ggtgtgagcc gtggatggtg atgggcagga aggaagagct aggtctgagt
     8881 gctgatagga agtaggctgc ttgagttccc ccaatgctcc cattttaact cccacactgt
     8941 ttttcaaaat cacatttggt ttgtgcctgt taggaggagt tgccccggta aggcgcataa
     9001 cgataccacg atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc
     9061 ctattccgaa gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac
     9121 cctccacact tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga
     9181 agaacccgtt gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt
     9241 gggtggcctg cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg
     9301 ccaccaaggt cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga
     9361 agactgaagg ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc
     9421 aaggaaacaa acttggcctc accaggcctc agccttggct cctcctggga actctactgc
     9481 ccttgggatc cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact
     9541 ggctagccta ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt
     9601 ccctaggata gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag
     9661 attttggctg ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc
     9721 ttttagctac atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg
     9781 gtcagtgggc acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt
     9841 atggccttgg gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt
     9901 gggtgcgttg gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct
     9961 cctccatgac aaccaggtcc caggtcccga aaaccaaaga agaagaaccc taacaaagag
    10021 gacaagcggc ctcgcacagc cttcactgct gagcagctcc agaggctcaa ggctgagttt
    10081 cagaccaaca ggtacctgac agagcagcgg cgccagagtc tggcacagga gctcggtacc
    10141 cggaagatct ggactctaga gaattccgcc cctctccctc ccccccccct aacgttactg
    10201 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
    10261 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
    10321 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
    10381 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
    10441 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
    10501 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
    10561 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
    10621 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
    10681 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
    10741 agcttgccac aaccatggaa gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg
    10801 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
    10861 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
    10921 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
    10981 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
    11041 acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
    11101 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc
    11161 gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg
    11221 gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg
    11281 gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc
    11341 aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta
    11401 cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac
    11461 tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt
    11521 tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg
    11581 atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac
    11641 tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg
    11701 gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg
    11761 gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc
    11821 atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga
    11881 agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca
    11941 cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca
    12001 tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg
    12061 taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga
    12121 atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc
    12181 cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt
    12241 gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca
    12301 tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc
    12361 acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc
    12421 cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg
    12481 aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc
    12541 agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag
    12601 caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca
    12661 gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg
    12721 gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga
    12781 ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg
    12841 tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt
    12901 ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc
    12961 tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc
    13021 gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc
    13081 tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc
    13141 gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag
    13201 cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc
    13261 acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg
    13321 gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg
    13381 cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat
    13441 tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc
    13501 tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg
    13561 ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca
    13621 gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag
    13681 aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga
    13741 gcccgtcagt atcggcggaa ttccagctga gcgccggtcg ctaccattac cagttggtct
    13801 ggtgtcaaaa ataataataa ccgggcaggg gggatctaag ctctagataa gtaatgatca
    13861 taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    13921 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    13981 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    14041 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgga
    14101 ataacttcgt atagcataca ttatacgaag ttatgtttaa acggcgcgcc ccggaattcg
    14161 ccttctgcag gagcgtacag aacccagggc cctggcaccc gtgcagaccc tggcccaccc
    14221 cacctgggcg ctcagtgccc aagagatgtc cacacctagg atgtcccgcg gtgggtgggg
    14281 ggcccgagag acgggcaggc cgggggcagg cctggccatg cggggccgaa ccgggcactg
    14341 cccagcgtgg gcgcgggggc cacggcgcgc gcccccagcc cccgggccca gcaccccaag
    14401 gcggccaacg ccaaaactct ccctcctcct cttcctcaat ctcgctctcg ctcttttttt
    14461 ttttcgcaaa aggaggggag agggggtaaa aaaatgctgc actgtgcggc gaagccggtg
    14521 agtgagcggc gcggggccaa tcagcgtgcg ccgttccgaa agttgccttt tatggctcga
    14581 gcggccgcgg cggcgcccta taaaacccag cggcgcgacg cgccaccacc gccgagaccg
    14641 cgtccgcccc gcgagcacag agcctcgcct ttgccgatcc tctagagtcg agatccgccg
    14701 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
    14761 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
    14821 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
    14881 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
    14941 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
    15001 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
    15061 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
    15121 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
    15181 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
    15241 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
    15301 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
    15361 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
    15421 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
    15481 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
    15541 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    15601 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    15661 cttcgcccac cccccggatc taagctctag ataagtaatg atcataatca gccatatcac
    15721 atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    15781 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    15841 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    15901 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgggggtacc gcgtcgagaa
    15961 gttcctattc cgaagttcct attctctaga aagtatagga acttcgtcga gataacttcg
    16021 tatagcatac attatacgaa gttatgtcga gatatctaga cccagctttc ttgtacaaag
    16081 tggttgatat ctctatagtc gcagtaggcg gagttcctga cactaaacct cactggggat
    16141 agaactcagt agtagagcat tcaggcttct ctgtggccct agattcactc cctagcatga
    16201 gagacagaca gagagacaga gacacacaga gacagagaca gagagacaga gagagagaca
    16261 gaggcagaga gagagagaca gagagacgga gagagagaga gagaccaaaa gagacaggtg
    16321 gacagacaga cagacactgg tagaccaaat tttccacaag acttttccta gattccctac
    16381 actgagatct ggaggagcca ggcccataag tgtaaaggta gaccaaaatg agaggtgcct
    16441 cctctcctag catcgtgcgc cttattccag ggacctggtc gatgaagcaa aggactatca
    16501 tctgatgcca gagcgccggc cccacctgcc agctttcagg actcggcccc gatgctgcac
    16561 gtccatcgct gggctcatct acgctgtggg gggcctcaac tcagcaggta tcttgcagct
    16621 cccctttgca aagcccttcc tagagtgaat gaagaaaccc agtcaagcta gcctggttgt
    16681 tctggttcct cctctttatg ctgttctgtc tcccgtcctt atcttaagac accagagagt
    16741 agccctagag tagggcttat tatggattca taatattgaa ttgatgtcaa tgctaaaggt
    16801 gccttgaaga agagatggcg caacgcaatt aatgataact tcgtatagca tacattatac
    16861 gaagttatgg tctgagctcg ccatcagttc aatgctgaaa catcagcagc tccattacac
    16921 acggtattac ccagtacttc agagcctaaa gcaggagggt ctggagtttg aggccagcct
    16981 gggctacagt tattgagact ctgcttccaa aaacaaaaca gtacaaaacc accattacct
    17041 atagctcttg tactatagac agtcacattt ctaggaaagc ctcctggggg gtcaggggga
    17101 aattgacaga tctgttttca gttaaggtta ggaaaggtag ctagtgacct tctgcttgtt
    17161 aacagcataa cctagaaaag ataagtcttc agaaacaaac actggatgta acaagggctt
    17221 ggtccacgca agccacaggg aagataattg tctgagttgc taatcatgat ctggggagga
    17281 tgagagggtt cctgggttct gtggctgctc tttactgagt tctaagtatg ttctagatcc
    17341 atgcagggtg ctgtatccat tatgtcattt aaccctcatg gcagttctgg tagcctttaa
    17401 cttcacatcc aaaacagcat gaggacccaa tgcagcccgt ctggtcatta gatgaagtgg
    17461 ccggtagcac tgggacccat gcacttgact ctggcatgct ttctgcattg ggcagaccat
    17521 cctgtgcatt tgccctcatt gtcgatttca agcttacctg attatgactg agaactactc
    17581 tcattaaaga gcaaatgttt cccatctgag tctaaaatct ttattatagc tagagttttt
    17641 agtgggccca cttactctga gtaatttgtg ggaagagcca ataggattga gtcagatgtt
    17701 ccgtatagtc aatacccagt agctgtgagc taaacaaatg gtctcatttg ttttaatttg
    17761 agtagcaaat ttttatgcag gtgattccct gaatgtggtg gaagtgttcg accctatcgc
    17821 caatcgctgg gaaaagtgcc atcccatgac aacagcccga agccgtgtgg gtgtggctgt
    17881 ggtgaacggg ctcctctatg ctatcggggg atatgatggt cagttgcggc tgagcaccgt
    17941 ggaggcctac aatcctgaga cggacacatg gacccgagtg gggagcatga atagcaagcg
    18001 aaggtattct ggaagccaag tgcaggctga gcacacactg agcatctggg gagccccatg
    18061 taggggcaca taataaagta aatgcctggg aagtcctgct gccaggccag ccactgccag
    18121 cacctcctgg gagcaagccc ccatctgctt aagcataaaa tacctgcctt gtcctttgag
    18181 gaacttgggg agccaagaca tggacatgtt atgtaagtgg cagctaagga ctgaaaatgt
    18241 gtgtaatgga agattccagg agggcgagag actagactgg aagatctggg aagaggtggg
    18301 attaaagttg aagcctgaag gataaggagt tttaacctca ttagtaatgg aggaagcccg
    18361 gggtggtttt ctctgtgctt gctttctcgt gccatacata cgctcccgct gccccggaag
    18421 cgcacactct ccagacgtga gcacgcgtgt attcacgagg tggactgaac ttgtacatca
    18481 tccaacatgg tctagctatc cgtgtctccc accgctcgcc tgctcatttc caggatgaga
    18541 agtatggctg gaggacaccg tggtagtgcc cgagtgtaac cctcttagtc cagagatgat
    18601 gggcagtgtc cttccttacg tcaggcatgc gctgcagtgg taggggccca cactatttaa
    18661 agggtgtcaa tcagtcagcc acagcagacg agcagtcaca aatgttactc ttctggctct
    18721 cctcataggg tctgagaagg aaggaaggaa gcccaaggac ccttgcacat ttaaccagaa
    18781 caggtctgcc tgtatgttga gtttctatga cttgtactca attttaggga ggagaataat
    18841 gtgctgtttt taaaggatac tttgaaacct accagccact tgctctttaa aggtatttct
    18901 gtatagttct ctggcctagc cttggcctca caggcccatc ctccttgcca tacatctggt
    18961 tctctacttg ggagatcaca tgtctgggaa gggggaaaag atgccaagtg atggcacgtg
    19021 tggtgaggca gtggttagcg tggagggctt tgacgcccag aggcgatcca cgttgagcag
    19081 tactggcctg agccggaagg cacctgctca gcagcaggga gggcagagtt acagagtgca
    19141 cagaggtctg tgcgcatccc tgtagggaga agcagtgtat gcgcacgatg tgcttcttcc
    19201 aaggacgttc ccagagttct ctcttcccct ccccaccttt ctgtgtctac cctctgcctt
    19261 tgtacagttt gcagcttttt gtgaatgagg gacccctttc tcatatccca gccacaggtg
    19321 gctcttttac tttgacagcg tgtgtgtccc tctaggagac gttctggctt tgatgtcgga
    19381 gggtttgttg tgttatttaa atgggaggaa agttctctgg gcatccctct ccttcgtgtg
    19441 gctaatgccc tggtattagc aatatgggcc cagttccatt tgtccctttt aaattacagg
    19501 ttcccttaaa tgttcttagt cacttctgtt ctgacatccc gcatagctct ccatcctgcc
    19561 agtgacagct tcctttctgc tcgctttcca tctttgtttc tggtactgga ttgttccagc
    19621 ccatccctct tacttgactg ttgtctatgg tttgggagca cctcttgatt cattctccca
    19681 tcattgctca cctcctgact tagccgcctt gtgcttagga ccaaggcagc agctcagtgg
    19741 taggaagctt gctctaacaa gagcgaggcc cgaggttcaa ttcccaatgt tatataccaa
    19801 gaaaaaggaa atagagttac ttcaggtatg tttcacatcc atgtctagga gggggatcaa
    19861 tgcctattaa atttgtcatt ttcctaatga tcttccttaa gacaaggaag atctcctctt
    19921 agagcaggtt caaagggctg ttactgtagt ttgacttgct atgtatactt gaaaagtggc
    19981 agtttccagt ttttagtctc tgtagcatca acactttgtc aggcagcttg gcattaccgc
    20041 ttaaagtctc acaaggttta tatagggttg aaggttatct gtgcttacct agaccctggg
    20101 taatcaggat tttagggaag cttatgtgtg gaacagggga ctctgggctt gtgttgttta
    20161 gctctcaggc atgtcttctc caagcagcct tgagaagagc ccagcaggcc atgcttacag
    20221 caattcttcc ttgaagggtg atgcataagt gtataacaaa cagaagctgg aatctcctcc
    20281 accaaggcca aggaggggct gtgcagagcc aggaatcaca gaagcacagg ctaaactaca
    20341 aaggaactga gtaggaagtc agaggcaaca cacatactac ctcatctcat ggtgacacat
    20401 gagtacccat catcaggagt ttacctcatc tcgtggtgac acatgagtac ccatcatcag
    20461 gagttttggc tgttcatttg ttacttttgc ttgtttgatt gattgatttt tttttttctt
    20521 aaaaaaaaaa aggctcttgc tatgtagccc aggctgctct ctaacttgag ccctccctgc
    20581 ctcagcctcc tcagccctgg gattgcacca ccatgccgag cctttgttct ccactcataa
    20641 aaccattgtc taaaacatta tgccttctac atggctgatg aaactctggt gaatgaatag
    20701 atgtgacatg tgtcctaata gagaagccac tcttggagca tggggtcaga aggtagtaat
    20761 tctgaaagtg tgtgactgaa cccacagtta aattaggctg aggccctggg tttgctaggt
    20821 gggccaaagt gcttactata cagacctgat gaatttgtgc ttagtcctaa gaacccacag
    20881 tggaaggaga gacccagctc ccagatgctg tcctctgatc tctgcacatg tgcacacgca
    20941 tacacacaca cattaataat aatacatttt tattctttct tgttatttat atctgtgttg
    21001 gagtgttttg cttttatgta tatctgtata gcacatgcct gtagttcttt ggaagagcag
    21061 ccagtactca acctctgagc catctctcca gcctaaaagt agtttggggt aaatcaggct
    21121 agtcacgacg gtgtcaccta taatctcagc agttggaaaa tggaggcagg gggattaggg
    21181 gtgcagggcc atcctcagct acataaacaa gtgaatgaac tcatcctgcc atgggatgag
    21241 aggtgagcac ttcaggcaga tcaagggctg aatgcctgag gaggagagag ctcgttgggg
    21301 gggggggggg gatcggggag gaagcaggag cagaggacca ctgaacagat ctttgtggtc
    21361 tgcccttacc tggttgtgtc cctgggatcg ggtctgttct agaaaaagac agttgacaga
    21421 ttagtttctt agcgtgtgtg agaggctgct gggatgacca ttctgtctgt ttcagtgcca
    21481 tggggacagt cgtgctggat ggacagatct acgtgtgtgg aggctatgac ggcaactcct
    21541 ccctcaactc tgtggagacc tactcacctg agacggacaa gtaaggctga cttatccccc
    21601 tgcctcagct tcttgagtgc tgaaattaca gatagaaact attatactcg gcctaccttc
    21661 cccacctcca ggcctttgtg cttgccaggc ttccagcccc cctcccccca cccccactcc
    21721 cacctcttgt ttagttttgc tttgctttgt tggagaaaag gtctcaacct caggactctg
    21781 atgagccacc tcagccacag agacatgccg tggcacggag cttaaagctt ctcctggcta
    21841 ggaagtgact taacaatact tgcatgtagt cgatgtctac atccttggct aaatgtcaga
    21901 cacggtgatg cgcccctttg atccctttac ttgaaggcag aggcaagtgt gtctccgtga
    21961 gttcaaaacc agtctgcttt atgtagtgca ttctagacta gccaaggcta caaagcagga
    22021 cttggtctca aaaaaacaaa acaaaaaaat atatttttta attccttgtt taagtgaaat
    22081 ttcatggtta aatttcaaaa gagctaaagc aatggtggtt caatc
//