LOCUS       KO-first_condition_ready_41213_MGI:2143315        22065 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Klhl18 targeting project(s): 41213
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 42066
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     exon            7788..7948
                     /db_xref="ENSEMBL:ENSMUSE00001249190"
                     /rank="5"
                     /note="ENSMUSE00001249190"
     misc_feature    4215..8929
                     /note="5 arm"
     misc_feature    8957..8981
                     /note="B1 Gateway"
     misc_feature    8994..9041
                     /note="Frt"
     intron          9042..9916
                     /note="mouse En2 intron"
     misc_feature    9914..9916
                     /note="Splice Acceptor"
     exon            9917..10089
                     /note="mouse En2 exon"
     misc_feature    10101..10694
                     /note="ECMV IRES"
     gene            10695..13755
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13794..14037
                     /note="SV40 polyadenylation site"
     misc_feature    14041..14074
                     /note="loxP"
     promoter        14101..14620
                     /note="human beta actin promoter"
     gene            14646..15440
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15640..15881
                     /note="SV40 polyadenylation site"
     misc_feature    15898..15945
                     /note="Frt"
     misc_feature    15952..15985
                     /note="loxP"
     misc_feature    9931..10008
                     /note="FCHK std_gpr"
     misc_feature    16001..16021
                     /note="B2 Gateway"
     misc_feature    8930..16051
                     /note="Synthetic Cassette"
     exon            16411..16547
                     /db_xref="ENSEMBL:ENSMUSE00001219951"
                     /type="targeted"
                     /rank="6"
                     /note="target exon 1 ENSMUSE00001219951"
     misc_feature    16052..16751
                     /note="Critical Region"
     misc_feature    16775..16808
                     /note="Downstream LoxP"
     misc_feature    16752..16831
                     /note="synthetic loxP region"
     exon            21416..21520
                     /db_xref="ENSEMBL:ENSMUSE00001212528"
                     /rank="8"
                     /note="ENSMUSE00001212528"
     exon            17721..17943
                     /db_xref="ENSEMBL:ENSMUSE00001268000"
                     /rank="7"
                     /note="ENSMUSE00001268000"
     misc_feature    16832..22065
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggatattga catttttctc tggtttgttt ctgtcttctc agttgagagg
     4261 gagcctgagg ggagaatatt agaaatctgg gggaatgaaa tatatgagat ggacagatga
     4321 gcctgggaga ttgagaaggg cagagagatg gagaggggca gggagatggg gctgatgatt
     4381 tggcttaaac gtaaagtgct cagctccatg ggtgttctct gtcagtgtcg catattggtg
     4441 caggtattga ataaaaggca cactgaactt gaagagctgg actttaaagg ctggagaggt
     4501 ggtccagtgg ttaagcgtat ctgctgttct tccaggggac ccatgtctga tttcctcatg
     4561 ttgcagggct taaaaccacc tatagctcca ggaaatcagt caccctatct gtcctatggg
     4621 caccctacac acatgtgaca cacacactca cacacactca catacacaca cattaattat
     4681 ttaaaataaa tctttaaaaa tatgcggcct ttatgaaatt agcagtggtg gtggtgtttt
     4741 gttttgtttt gttttttcta gagtgagcat gtccaaagga gggaggagct tggcacagga
     4801 atggaggaca cccatgtagc catctctaga acacaggtca gcagagaggt gaggaactag
     4861 aacagatcta gagagctgac agggaggagg accaggcaat gtgagaggca atcagggatt
     4921 tctgagctgg aagaggtgga agtggtgttg aagtatgggt ggacttgaaa ctctggaatc
     4981 agggtcaggc atgggatcta cttggagaga gcttgcctac catgaacaaa gccctcgatc
     5041 cagttcctag taccacataa agaagacatg gctgtgcaca tttgtaatcc cagcactgga
     5101 gacgaaggca gcagacttac aagtttatgc ttatctttaa ttgtataaca agttgggact
     5161 gggcataggc acaaatcttt catcttagca tgtgggaggc agagacagga gaatctctat
     5221 gagtttaaga ccagcctgtt ccacatagca agttccaggt cagctacaaa gcaagttcca
     5281 gactagttaa gtagtgagac tctatctcaa aaacaagttg gaaactagcc tgggaaacac
     5341 acacacacac acacacacac acacacacac acgctcgcac acacaaacac atacacctcg
     5401 ctatgaaatt gggaagttct tgaaatctgt ttataacaac cattttgtat ggtggcacta
     5461 ttggtaaagc aaagtaaaag tcttaccttc ttggagtggt caagaaaaat taaaataaaa
     5521 gcagattgtt atcaggcagg gcaaggaaga gcaaaggatg cacctatcag cagaggtgta
     5581 gtgtaagtag ccttaggaag acagtgtgca gcaaccacac ggtggctcac agccatctga
     5641 aaacagctac agtgtactta catataataa ataaataaat aaataaataa ataaataaat
     5701 aaataaattt ttttaaaaaa ggaagctagt gtgtgatgtt tgggacagtg ttgagggcta
     5761 gcagtgaagg acattgcatg cacggtaaca agtttggttc agcaaactcc tggggtcatc
     5821 actattgcct ttgatccact gagaagtcca ataaaaatgt aggatgtttg tagccaagag
     5881 ccgtagcctg gttgagtggc tgaagagttt gttcttctag acgggggacc tgaattggaa
     5941 tccgtagagc ctacattaag ctaggcacag cagtgacctc cggtgggtcc cagtactctt
     6001 tttgtaaaag gggaagcaaa gacagaaagg ttccaggaag cctgtggtcc aactagtctg
     6061 ctgtacacag ttagcaaaca acaagatggg agaggagggt tgtcacccaa ggttgtcctc
     6121 tgacctctat ccatgtggtg ctgtggcaag cgtgtgccgg gtgcatacaa tgggggaggg
     6181 gaggagttac tgctgtgaat agtgcctggt ggggaggact gcggatgaga aaataaagaa
     6241 ggcagaaacc tgggattgag gttttgcata aactgatagc ttatgtcaag caagtgtatt
     6301 aaatagtata gcattctgcc gggcagtggt ggcgcacgtc tttaatctca gcactcagga
     6361 ggcagaggct ggcagatctc tgagctcaag gccagcctgg actacagagt gagttccaag
     6421 acagccaggg ctgtactgag aaaccctgtc tcaaacaaac aaaagaagaa tagcatcttg
     6481 catattgttt acagcggggg gggggggggg gggcgaatgg gccacggtca gcaggaagct
     6541 aagtcagaca atgtggcaag agaacacagg atacctcaga cacagctgcc tcaggactga
     6601 taactgcagt ctttcatggg gaaaaggcga tttccccagg aaccaaaacc ttaatatgtg
     6661 agcctggccc cagccccata ctagaccttg ccaagtctgc ccctgggcaa ggacagagct
     6721 agagttctct ttgtcctttc attagggctg tgagaaatct tggattcatc taactcccaa
     6781 cactaggagg cagccgtcga gatgctaaca caaactcagc taaattccgt aactatctga
     6841 agataacctg ccccaccctt aactattagt gacaggtaga gaagtggccc ctgagcttag
     6901 cagcatggac agagacttag aagcacggga tcctaagaac agaatgttta cctcctaggt
     6961 tcttccagta accaatgtat cctggagcag cccacgcgag tttatttgag tagcaatctg
     7021 cgaggcctga ggggcttcaa ctggttatat tcttgtgttt gaaggaataa ggaaggttgc
     7081 taggtggaca gtgtaaacag aggaactggc ctggcctgga atttagagct ggttggatgc
     7141 aagatcaacc aggaaaaaga tgttctgttg tgatgcggaa aagaatcgat aatgaaatcc
     7201 aggaagcttt ccagtaaccc aggcttacat tacagaccag tggacctaag ctttccgtgg
     7261 tctcgtgtgc ctggaactct agcacttggg agcatcttga atccttttag gccagcctgg
     7321 gctgcatcgt aaactgtctc aaaaacaaaa gactaggcct aagagcatag ctggaggagt
     7381 gtaaagaaat ctgtggatgg gctggagaga tggctcagcg gttaagggca tcaactgctc
     7441 ttccagaggt cccgagttcg aatcctagca accacaaggt ggctcacaac catctctcat
     7501 gaaatctgat gccctctctg gtgtgtctaa aaacagagat gtactatcac tagataggta
     7561 ggtaggtagg taggtaggta ggtaggtagg taggtaggta ggtaggtaga tagatagatc
     7621 ttaaaaaaaa caaaaacaaa agaaagcagt ggattgcaga catgggtggg aggaaagctg
     7681 gatcgcctga ggacctgcct cagtacaggc agtggaagaa ctgagagcga ggaggcagga
     7741 gcgtggtctc tgggtagctg caagagcatc tgctctttgt cccataggtt tttgaagctg
     7801 cattggcctg ggtcaggtat gaccgggagc agaggggacc atgcctgccc gagctgctgt
     7861 ccaatatccg cctgcctctt tgccggcccc agttcttatc agatcgagtg cagcaagatg
     7921 acctggtacg gtgctgtcac aaatgcaggt aagtgtgggt ggccttgtcc aggacacggc
     7981 ccagcaaaga ggaggaattg gccacctgtc gtagctcagg ggaggaagga tttggagcag
     8041 gcagttaact cacacattgc tcacaagcag agagatgcca tgtaggagca gaaggggctt
     8101 ttcttcccta agttatctat gtgttcttca gcttcatcca ctgagagtta tgaatgtact
     8161 ctgtgtatgc tgcccacact ggcagctgac ttgatagtac aaatggggct tcccggaggc
     8221 tccctgccct ctgcaataaa ttgtagcttg tgactagcct ctgatgagtg atgggggggg
     8281 gggcttgtat accattgtgt agagcttctg cctagagcca ccagcacagg agatgggttg
     8341 ttaagcaatt taaacaaaga aggcctgtct tcccagttgc ttgttatagc cacaatgctg
     8401 ggttttggtt ttttccttta ctgacaagat ttttacacat tgagtttccc atgattccac
     8461 ccctagttgg gatcagcagg ttccgggagt aagcagtggc gtggtacagg gctttgtgat
     8521 agtatgtcag ggagcaagaa ctgtgtggct ggagggagcc ggggccctgt gacaagcagt
     8581 ctgaaggata atatcgaggc atacgaacta ttctgtcagc agtaggtagc taacaaaaga
     8641 aggttcttag agaaaagcaa tgtagtctgt ggtttggagg ggctggggag ggcaagccag
     8701 ggcctgagaa atccatctgg agcacttgac agcagttgcc gaggtgcaag caaggggtga
     8761 ttgacaggag ggtgtgagcc gtggatggtg atgggcagga aggaagagct aggtctgagt
     8821 gctgatagga agtaggctgc ttgagttccc ccaatgctcc cattttaact cccacactgt
     8881 ttttcaaaat cacatttggt ttgtgcctgt taggaggagt tgccccggta aggcgcataa
     8941 cgataccacg atatcaacaa gtttgtacaa aaaagcaggc tggcgccgga accgaagttc
     9001 ctattccgaa gttcctattc tctagaaagt ataggaactt cgaacccttt cccacaccac
     9061 cctccacact tgccccaaac actgccaact atgtaggagg aaggggttgg gactaacaga
     9121 agaacccgtt gtggggaagc tgttgggagg gtcactttat gttcttgccc aaggtcagtt
     9181 gggtggcctg cttctgatga ggtggtccca aggtctgggg tagaaggtga gagggacagg
     9241 ccaccaaggt cagccccccc cccctatccc ataggagcca ggtccctctc ctggacagga
     9301 agactgaagg ggagatgcca gagactcagt gaagcctggg gtaccctatt ggagtccttc
     9361 aaggaaacaa acttggcctc accaggcctc agccttggct cctcctggga actctactgc
     9421 ccttgggatc cccttgtagt tgtgggttac ataggaaggg ggacgggatt ccccttgact
     9481 ggctagccta ctcttttctt cagtcttctc catctcctct cacctgtctc tcgacccttt
     9541 ccctaggata gacttggaaa aagataaggg gagaaaacaa atgcaaacga ggccagaaag
     9601 attttggctg ggcattcctt ccgctagctt ttattgggat cccctagttt gtgataggcc
     9661 ttttagctac atctgccaat ccatctcatt ttcacacaca cacacaccac tttccttctg
     9721 gtcagtgggc acatgtccag cctcaagttt atatcaccac ccccaatgcc caacacttgt
     9781 atggccttgg gcgggtcatc ccccccccca cccccagtat ctgcaacctc aagctagctt
     9841 gggtgcgttg gttgtggata agtagctaga ctccagcaac cagtaacctc tgccctttct
     9901 cctccatgac aaccaggtcc caggtcccga aaaccaaaga agaagaaccc taacaaagag
     9961 gacaagcggc ctcgcacagc cttcactgct gagcagctcc agaggctcaa ggctgagttt
    10021 cagaccaaca ggtacctgac agagcagcgg cgccagagtc tggcacagga gctcggtacc
    10081 cggaagatct ggactctaga gaattccgcc cctctccctc ccccccccct aacgttactg
    10141 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
    10201 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
    10261 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
    10321 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
    10381 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
    10441 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
    10501 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
    10561 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
    10621 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
    10681 agcttgccac aaccatggaa gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg
    10741 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
    10801 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
    10861 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
    10921 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
    10981 acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
    11041 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc
    11101 gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg
    11161 gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg
    11221 gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc
    11281 aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta
    11341 cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac
    11401 tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt
    11461 tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg
    11521 atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac
    11581 tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg
    11641 gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg
    11701 gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc
    11761 atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga
    11821 agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca
    11881 cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca
    11941 tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg
    12001 taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga
    12061 atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc
    12121 cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt
    12181 gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca
    12241 tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc
    12301 acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc
    12361 cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg
    12421 aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc
    12481 agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag
    12541 caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca
    12601 gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg
    12661 gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga
    12721 ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg
    12781 tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt
    12841 ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc
    12901 tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc
    12961 gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc
    13021 tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc
    13081 gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag
    13141 cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc
    13201 acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg
    13261 gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg
    13321 cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat
    13381 tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc
    13441 tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg
    13501 ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca
    13561 gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag
    13621 aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga
    13681 gcccgtcagt atcggcggaa ttccagctga gcgccggtcg ctaccattac cagttggtct
    13741 ggtgtcaaaa ataataataa ccgggcaggg gggatctaag ctctagataa gtaatgatca
    13801 taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    13861 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    13921 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    13981 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgga
    14041 ataacttcgt atagcataca ttatacgaag ttatgtttaa acggcgcgcc ccggaattcg
    14101 ccttctgcag gagcgtacag aacccagggc cctggcaccc gtgcagaccc tggcccaccc
    14161 cacctgggcg ctcagtgccc aagagatgtc cacacctagg atgtcccgcg gtgggtgggg
    14221 ggcccgagag acgggcaggc cgggggcagg cctggccatg cggggccgaa ccgggcactg
    14281 cccagcgtgg gcgcgggggc cacggcgcgc gcccccagcc cccgggccca gcaccccaag
    14341 gcggccaacg ccaaaactct ccctcctcct cttcctcaat ctcgctctcg ctcttttttt
    14401 ttttcgcaaa aggaggggag agggggtaaa aaaatgctgc actgtgcggc gaagccggtg
    14461 agtgagcggc gcggggccaa tcagcgtgcg ccgttccgaa agttgccttt tatggctcga
    14521 gcggccgcgg cggcgcccta taaaacccag cggcgcgacg cgccaccacc gccgagaccg
    14581 cgtccgcccc gcgagcacag agcctcgcct ttgccgatcc tctagagtcg agatccgccg
    14641 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
    14701 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
    14761 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
    14821 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
    14881 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
    14941 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
    15001 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
    15061 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
    15121 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
    15181 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
    15241 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
    15301 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
    15361 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
    15421 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
    15481 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    15541 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    15601 cttcgcccac cccccggatc taagctctag ataagtaatg atcataatca gccatatcac
    15661 atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    15721 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    15781 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    15841 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgggggtacc gcgtcgagaa
    15901 gttcctattc cgaagttcct attctctaga aagtatagga acttcgtcga gataacttcg
    15961 tatagcatac attatacgaa gttatgtcga gatatctaga cccagctttc ttgtacaaag
    16021 tggttgatat ctctatagtc gcagtaggcg gagttcctga cactaaacct cactggggat
    16081 agaactcagt agtagagcat tcaggcttct ctgtggccct agattcactc cctagcatga
    16141 gagacagaca gagagacaga gacacacaga gacagagaca gagagacaga gagagagaca
    16201 gaggcagaga gagagagaca gagagacgga gagagagaga gagaccaaaa gagacaggtg
    16261 gacagacaga cagacactgg tagaccaaat tttccacaag acttttccta gattccctac
    16321 actgagatct ggaggagcca ggcccataag tgtaaaggta gaccaaaatg agaggtgcct
    16381 cctctcctag catcgtgcgc cttattccag ggacctggtc gatgaagcaa aggactatca
    16441 tctgatgcca gagcgccggc cccacctgcc agctttcagg actcggcccc gatgctgcac
    16501 gtccatcgct gggctcatct acgctgtggg gggcctcaac tcagcaggta tcttgcagct
    16561 cccctttgca aagcccttcc tagagtgaat gaagaaaccc agtcaagcta gcctggttgt
    16621 tctggttcct cctctttatg ctgttctgtc tcccgtcctt atcttaagac accagagagt
    16681 agccctagag tagggcttat tatggattca taatattgaa ttgatgtcaa tgctaaaggt
    16741 gccttgaaga agagatggcg caacgcaatt aatgataact tcgtatagca tacattatac
    16801 gaagttatgg tctgagctcg ccatcagttc aatgctgaaa catcagcagc tccattacac
    16861 acggtattac ccagtacttc agagcctaaa gcaggagggt ctggagtttg aggccagcct
    16921 gggctacagt tattgagact ctgcttccaa aaacaaaaca gtacaaaacc accattacct
    16981 atagctcttg tactatagac agtcacattt ctaggaaagc ctcctggggg gtcaggggga
    17041 aattgacaga tctgttttca gttaaggtta ggaaaggtag ctagtgacct tctgcttgtt
    17101 aacagcataa cctagaaaag ataagtcttc agaaacaaac actggatgta acaagggctt
    17161 ggtccacgca agccacaggg aagataattg tctgagttgc taatcatgat ctggggagga
    17221 tgagagggtt cctgggttct gtggctgctc tttactgagt tctaagtatg ttctagatcc
    17281 atgcagggtg ctgtatccat tatgtcattt aaccctcatg gcagttctgg tagcctttaa
    17341 cttcacatcc aaaacagcat gaggacccaa tgcagcccgt ctggtcatta gatgaagtgg
    17401 ccggtagcac tgggacccat gcacttgact ctggcatgct ttctgcattg ggcagaccat
    17461 cctgtgcatt tgccctcatt gtcgatttca agcttacctg attatgactg agaactactc
    17521 tcattaaaga gcaaatgttt cccatctgag tctaaaatct ttattatagc tagagttttt
    17581 agtgggccca cttactctga gtaatttgtg ggaagagcca ataggattga gtcagatgtt
    17641 ccgtatagtc aatacccagt agctgtgagc taaacaaatg gtctcatttg ttttaatttg
    17701 agtagcaaat ttttatgcag gtgattccct gaatgtggtg gaagtgttcg accctatcgc
    17761 caatcgctgg gaaaagtgcc atcccatgac aacagcccga agccgtgtgg gtgtggctgt
    17821 ggtgaacggg ctcctctatg ctatcggggg atatgatggt cagttgcggc tgagcaccgt
    17881 ggaggcctac aatcctgaga cggacacatg gacccgagtg gggagcatga atagcaagcg
    17941 aaggtattct ggaagccaag tgcaggctga gcacacactg agcatctggg gagccccatg
    18001 taggggcaca taataaagta aatgcctggg aagtcctgct gccaggccag ccactgccag
    18061 cacctcctgg gagcaagccc ccatctgctt aagcataaaa tacctgcctt gtcctttgag
    18121 gaacttgggg agccaagaca tggacatgtt atgtaagtgg cagctaagga ctgaaaatgt
    18181 gtgtaatgga agattccagg agggcgagag actagactgg aagatctggg aagaggtggg
    18241 attaaagttg aagcctgaag gataaggagt tttaacctca ttagtaatgg aggaagcccg
    18301 gggtggtttt ctctgtgctt gctttctcgt gccatacata cgctcccgct gccccggaag
    18361 cgcacactct ccagacgtga gcacgcgtgt attcacgagg tggactgaac ttgtacatca
    18421 tccaacatgg tctagctatc cgtgtctccc accgctcgcc tgctcatttc caggatgaga
    18481 agtatggctg gaggacaccg tggtagtgcc cgagtgtaac cctcttagtc cagagatgat
    18541 gggcagtgtc cttccttacg tcaggcatgc gctgcagtgg taggggccca cactatttaa
    18601 agggtgtcaa tcagtcagcc acagcagacg agcagtcaca aatgttactc ttctggctct
    18661 cctcataggg tctgagaagg aaggaaggaa gcccaaggac ccttgcacat ttaaccagaa
    18721 caggtctgcc tgtatgttga gtttctatga cttgtactca attttaggga ggagaataat
    18781 gtgctgtttt taaaggatac tttgaaacct accagccact tgctctttaa aggtatttct
    18841 gtatagttct ctggcctagc cttggcctca caggcccatc ctccttgcca tacatctggt
    18901 tctctacttg ggagatcaca tgtctgggaa gggggaaaag atgccaagtg atggcacgtg
    18961 tggtgaggca gtggttagcg tggagggctt tgacgcccag aggcgatcca cgttgagcag
    19021 tactggcctg agccggaagg cacctgctca gcagcaggga gggcagagtt acagagtgca
    19081 cagaggtctg tgcgcatccc tgtagggaga agcagtgtat gcgcacgatg tgcttcttcc
    19141 aaggacgttc ccagagttct ctcttcccct ccccaccttt ctgtgtctac cctctgcctt
    19201 tgtacagttt gcagcttttt gtgaatgagg gacccctttc tcatatccca gccacaggtg
    19261 gctcttttac tttgacagcg tgtgtgtccc tctaggagac gttctggctt tgatgtcgga
    19321 gggtttgttg tgttatttaa atgggaggaa agttctctgg gcatccctct ccttcgtgtg
    19381 gctaatgccc tggtattagc aatatgggcc cagttccatt tgtccctttt aaattacagg
    19441 ttcccttaaa tgttcttagt cacttctgtt ctgacatccc gcatagctct ccatcctgcc
    19501 agtgacagct tcctttctgc tcgctttcca tctttgtttc tggtactgga ttgttccagc
    19561 ccatccctct tacttgactg ttgtctatgg tttgggagca cctcttgatt cattctccca
    19621 tcattgctca cctcctgact tagccgcctt gtgcttagga ccaaggcagc agctcagtgg
    19681 taggaagctt gctctaacaa gagcgaggcc cgaggttcaa ttcccaatgt tatataccaa
    19741 gaaaaaggaa atagagttac ttcaggtatg tttcacatcc atgtctagga gggggatcaa
    19801 tgcctattaa atttgtcatt ttcctaatga tcttccttaa gacaaggaag atctcctctt
    19861 agagcaggtt caaagggctg ttactgtagt ttgacttgct atgtatactt gaaaagtggc
    19921 agtttccagt ttttagtctc tgtagcatca acactttgtc aggcagcttg gcattaccgc
    19981 ttaaagtctc acaaggttta tatagggttg aaggttatct gtgcttacct agaccctggg
    20041 taatcaggat tttagggaag cttatgtgtg gaacagggga ctctgggctt gtgttgttta
    20101 gctctcaggc atgtcttctc caagcagcct tgagaagagc ccagcaggcc atgcttacag
    20161 caattcttcc ttgaagggtg atgcataagt gtataacaaa cagaagctgg aatctcctcc
    20221 accaaggcca aggaggggct gtgcagagcc aggaatcaca gaagcacagg ctaaactaca
    20281 aaggaactga gtaggaagtc agaggcaaca cacatactac ctcatctcat ggtgacacat
    20341 gagtacccat catcaggagt ttacctcatc tcgtggtgac acatgagtac ccatcatcag
    20401 gagttttggc tgttcatttg ttacttttgc ttgtttgatt gattgatttt tttttttctt
    20461 aaaaaaaaaa aggctcttgc tatgtagccc aggctgctct ctaacttgag ccctccctgc
    20521 ctcagcctcc tcagccctgg gattgcacca ccatgccgag cctttgttct ccactcataa
    20581 aaccattgtc taaaacatta tgccttctac atggctgatg aaactctggt gaatgaatag
    20641 atgtgacatg tgtcctaata gagaagccac tcttggagca tggggtcaga aggtagtaat
    20701 tctgaaagtg tgtgactgaa cccacagtta aattaggctg aggccctggg tttgctaggt
    20761 gggccaaagt gcttactata cagacctgat gaatttgtgc ttagtcctaa gaacccacag
    20821 tggaaggaga gacccagctc ccagatgctg tcctctgatc tctgcacatg tgcacacgca
    20881 tacacacaca cattaataat aatacatttt tattctttct tgttatttat atctgtgttg
    20941 gagtgttttg cttttatgta tatctgtata gcacatgcct gtagttcttt ggaagagcag
    21001 ccagtactca acctctgagc catctctcca gcctaaaagt agtttggggt aaatcaggct
    21061 agtcacgacg gtgtcaccta taatctcagc agttggaaaa tggaggcagg gggattaggg
    21121 gtgcagggcc atcctcagct acataaacaa gtgaatgaac tcatcctgcc atgggatgag
    21181 aggtgagcac ttcaggcaga tcaagggctg aatgcctgag gaggagagag ctcgttgggg
    21241 gggggggggg gatcggggag gaagcaggag cagaggacca ctgaacagat ctttgtggtc
    21301 tgcccttacc tggttgtgtc cctgggatcg ggtctgttct agaaaaagac agttgacaga
    21361 ttagtttctt agcgtgtgtg agaggctgct gggatgacca ttctgtctgt ttcagtgcca
    21421 tggggacagt cgtgctggat ggacagatct acgtgtgtgg aggctatgac ggcaactcct
    21481 ccctcaactc tgtggagacc tactcacctg agacggacaa gtaaggctga cttatccccc
    21541 tgcctcagct tcttgagtgc tgaaattaca gatagaaact attatactcg gcctaccttc
    21601 cccacctcca ggcctttgtg cttgccaggc ttccagcccc cctcccccca cccccactcc
    21661 cacctcttgt ttagttttgc tttgctttgt tggagaaaag gtctcaacct caggactctg
    21721 atgagccacc tcagccacag agacatgccg tggcacggag cttaaagctt ctcctggcta
    21781 ggaagtgact taacaatact tgcatgtagt cgatgtctac atccttggct aaatgtcaga
    21841 cacggtgatg cgcccctttg atccctttac ttgaaggcag aggcaagtgt gtctccgtga
    21901 gttcaaaacc agtctgcttt atgtagtgca ttctagacta gccaaggcta caaagcagga
    21961 cttggtctca aaaaaacaaa acaaaaaaat atatttttta attccttgtt taagtgaaat
    22021 ttcatggtta aatttcaaaa gagctaaagc aatggtggtt caatc
//