LOCUS       KO-first_condition_ready_28730_MGI:106927        23830 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Aff3 targeting project(s): 28730
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Pgk_P
COMMENT     design_id: 96513
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     misc_feature    4215..10100
                     /note="5 arm"
     misc_feature    10128..10152
                     /note="B1 Gateway"
     misc_feature    10165..10212
                     /note="Frt"
     intron          10213..11087
                     /note="mouse En2 intron"
     misc_feature    11085..11087
                     /note="Splice Acceptor"
     exon            11088..11089
                     /note="mouse En2 exon"
     misc_feature    11301..11862
                     /note="ECMV IRES"
     gene            11873..14919
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14954..15209
                     /note="SV40 polyadenylation site"
     misc_feature    15213..15246
                     /note="loxP"
     promoter        15278..15785
                     /note="mouse phosphoglycerate kinase 1 promoter"
     gene            15872..16663
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16861..17104
                     /note="SV40 polyadenylation site"
     misc_feature    17121..17168
                     /note="Frt"
     misc_feature    17175..17208
                     /note="loxP"
     misc_feature    11102..11179
                     /note="FCHK std_gpr"
     misc_feature    17224..17244
                     /note="B2 Gateway"
     misc_feature    10101..17274
                     /note="Synthetic Cassette"
     exon            19279..19664
                     /db_xref="ENSEMBL:ENSMUSE00000700850"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00000700850"
     exon            18847..19159
                     /db_xref="ENSEMBL:ENSMUSE00001265694"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00001265694"
     exon            17624..17744
                     /db_xref="ENSEMBL:ENSMUSE00001299434"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 3 ENSMUSE00001299434"
     misc_feature    17275..19825
                     /note="Critical Region"
     misc_feature    19849..19882
                     /note="Downstream LoxP"
     misc_feature    19826..19905
                     /note="synthetic loxP region"
     misc_feature    19906..23830
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggatgcctt tctgtccacc aagtctgtag atggagggga tttatgaata
     4261 acatctcaac catcttgctc tcctttcttt tgagacggga ttccgggtct tttattgttt
     4321 actttagaaa taacgaaaga tgtaattagt ttgactcatc aaatctaaaa caattgttaa
     4381 aaattcgggg cgtttgatta gactccttta attatacaca gcgtctcatg ttgccggctg
     4441 agagcagcgg agtttttgag gcaccgcagt ctccttcttg ccaccagttg cttccctctc
     4501 cctgttcacc ttctgggtgt tcatacccat cagtgtgctg gcccagagct caaagtgtct
     4561 gaaaaatggc ctccgtggta ggctgctcag ctatctttga tggagaatgg aagaagggga
     4621 gagcgttccc tggggttcct acatcctctg ctcttgggag aggctagaga aacctgctct
     4681 gaggagagca gtcaggacag gcagtgggta aacctaggct gtgggattct gaaaacagag
     4741 ttctagccaa ggaagtcatg ttgcctgcca agggtgaagg tcgccgcccc aagctctgga
     4801 aagccccccg cccccagcag tgatctgtgt tatggaccac tcagtggttt ctcttctacc
     4861 cgaccctcct acggggccca gctcagagct tgtctacata cctggtaccc acgctgctta
     4921 tgagtgagct ccttcctcct ttctctcttg ttctgagatc ttctcagcag taggaaggat
     4981 tggccgtagc cccgagtatt tccctcacat atagaactga ggagttggct ttggactaag
     5041 ctgtctttct gtgtttgaaa gatatcctct ttaaatacat ggttaccaga ctaatatccc
     5101 taaaggtcag gagatatgac tctcgtctca actcttacct agtccctgcc atggtttgga
     5161 gatggcatga gtgaattccc ggaggcccct ttgttgaaag catggtgcct tgtgctgtgg
     5221 ggctggaact ttttgaaaag gtggcatcta gtctaggtgt tctctgcatt aaaaaccact
     5281 cttctttcct agaccgttga gttgtaaggg tgagttgagg cataagtggc atggatggta
     5341 aatcctttgg gtctttctta tgaaccccca aatgatgcta tctactatgc tacaatgtag
     5401 ccagaaggcc atcactgctg attaaaacca tctgaccttg gatgttcagc ttcccaaact
     5461 gaaacaaaaa ccaaaagccc ccatttcttt ataaggtata ctgcctcagg tgttttgtta
     5521 taacaacagc caatgctgtg aatgctaaac ccctaatttg gagacacacc gtaagttagg
     5581 atgatctttt tataaagagc aaaacatgat tccagtcaag ggctgaggtt ggctccaata
     5641 taccctttgc tgacagccac ctccatggga aatggcagtt ggtttggaat gagtgctcct
     5701 atgtgggtgg gctacacgtg ggtgagtgct tgtcctggcc agctgaattg ggtaggtcaa
     5761 gcactgggct cagctgtccg ggttggctgc actcatcagc accttttctt ccagcgagca
     5821 cagtgtggga gagactggga taggcaatgc tgtcagcacc agttgtgact gtttaggatc
     5881 tggaattctg ttgtctgttg caagtctagg atgcatggta atcctgtgtg tgtgtgtgtg
     5941 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtc tgtagggctc cttagaaaaa gataattttc
     6001 tgttgtttgg aaactgggaa aaattgtaac tactaacata cttttcttca ttgttctttt
     6061 tgtatgggga atagcttctt tgtttcaact atcctatttg tttctgtgtt ttctgttaat
     6121 tcacccttgg cttatggaca tggtcttggt ttgtggacac ctccttctct ctcggtggcg
     6181 atgagtaaag tgctttggac tttgctgtct cttctgcatg tcccttttta aggatctaag
     6241 atgagcggtc taactcttct tctaaatacc ctgctccatt tagcttcctc atgtttgaga
     6301 caggactcac ttcttcccat cttcctcttg ctcccatctt cctcttgctc ccatcttcct
     6361 cttgctccca tcttcctctt gctcccatct tcctcttgct cccatcttcc tcttgctccc
     6421 atcttcctcc tgctcccatc ttcctcttgc tcccatcttc ctcttgctcc catcttcttc
     6481 ttgctcccat ctttctcctg ctcccatctt cctcctgctc ccatcttcct cttgctccca
     6541 tcttcctctt gctcccatct tcctcttgct cccatcttcc tcctgctccc atcttcctcc
     6601 tgctcccatc ttcctcttgc tcccatcttc cccctgctcc catcttcctc ctgctcccat
     6661 cttccaagga ctgagaggaa ggaagtgtgt agccatgctg gagcccttga ttttgaaaag
     6721 ctagcctagt aataatacct cctacccctg tctttatcta acagggattg taaggacact
     6781 cagcagacag acttcaggtg gctgtctagg tgtttgtggc aggaggaaga tgtccccaga
     6841 gttacaaaca atgttactta gtttcatgtt ttcaccttgc atttaaggat agattagttt
     6901 tggacattat cttgtttaga tgtttcctct tacccatgtt ccatactggc tttggcttga
     6961 gcccaacaag agacccggct taatagaata aaatcaagta aatgaagaac aatcagaaat
     7021 atttttatct ttttaaaatt tgctttaaaa aaaaacctaa acgtaatttt atttattttg
     7081 tttttttaag gagttgattt aaaagattcc gttttctggg actgttctgc attcataata
     7141 aataataaat aacattatgg cggttgactg ccacacttct gtgcaaaata aataaatgca
     7201 tttggtgata tacatttcgg gggtgtgatt cctgtgattt ctatggaaat aagggaaaac
     7261 tttaacagtt gatagaatct ttaccatctg ttagcaaggc agaaacggcg tggcagttac
     7321 tgagtttacc aggtacacca gtctgccagg aatgaggatt atctaggacc agtctgacat
     7381 cctgaggaag ccgacccact tgccaccttc aggggaaagg tgcctttggt actaaagcca
     7441 atggcacagg cctcagattg ctgcaggggc aaggaagtga ggggtggagg ccgctctctc
     7501 agggtgatag agagcccgtg ccagcttgtg cgtggatgct ttctgtaatt agtgcagagg
     7561 tggactagtt aagccaccac tgtctatagg agcaaaaggg ctgagcactg gtgagggatt
     7621 gggaaacgtg ggagatcctg cctttctctc agagtggcgt ttgctgtgag caggtgctat
     7681 tttagctcct ccagcctgac atcagacctg ggggcttcat gtctagttaa gaaggagagg
     7741 gtgggatgcc tctgctcagt ggaggctgta gaagaagcag agaccaaaga ggaggcatgg
     7801 aggaagctgg tcctttcttt tgtgtttcct ttaatctcct ttggttacgt gactatggtc
     7861 ctacacaggg tcatctgatg gcgtgaatgc actgtgcagg ctttgtggtg gccctgagaa
     7921 agggcggcag gtgcagctat tctgttggat gtcctgaccc atgtttgtaa gggtacttgg
     7981 cccttctgcc tgaaatcata catagtctgg ttgatgggct gaaccacaca gaaaactttt
     8041 attcagccat agactgcatc ttggactggt tttgattttg tctcttcagg cccaaagtgg
     8101 ctgggaaaga gggaggggtt catttacacg ggaacagctt ttcctcagct ccagcttgat
     8161 ctgactctta ctccaaagtc ctggggagcc actgagctga ctgtggtatc cactcccttg
     8221 ccttcatctc agacatctaa accatgttgc agaataagct gtgttttttc ctctgtccta
     8281 acactgcatt gggggtggga ggagcagtgt cacacctccc atttggatcc cagtgtccat
     8341 gtgcagcttc ccataggcct ttgaggggaa taccggcaga cctgtacgtg caacgatacc
     8401 acatgcaggc ttgccaggtt taaagcgctc tctctttctt cttgtggtgt ttgtatttct
     8461 tacaggggtg aagatatcct aagccagagg aatgattctc tagttctgga agttcaggca
     8521 tcagccagca gatgcaggag gtatcattct gggtgtgtgg ggcctcctca gaagcccacc
     8581 ttgttcatcc tgcataaggc cattccatgc atcaccccag tcttgtgtgt gcttggcttg
     8641 gcctgcatgg catgtgtgtg gaggtctctg tttgaaggtc ggcaccccag gtgccggagg
     8701 agggaagttc agaggcgtgg gatataaagt ctttatttac cccccttcaa atgccagccg
     8761 atgtaagtct aaaaggtaga agttggtgat agactagagt tgtcctgttg ctcaaagccc
     8821 acttcatgcc cctgtaccat aaatcatgtg tccttccatc ctgtcagtgt gtgaaaccca
     8881 tgcagcgttt acagaagccg gggactcagg gcaagcaact accagcacac gacagtcaca
     8941 gccatccctt tgggcttgca tgagtgtgtt gggggttcca cattgcgctg ctcagccatg
     9001 ggctgtgaca tcggaaggat gagcctctct gttggtagac gatttttttt tttaatgtaa
     9061 ggacaccggt taggctcccg aacctaataa ctttgggtaa tgtactaaga atgaattaaa
     9121 atgctaatgg cctccttgct ctggatattt tagcatttcc tgattttaaa tttaaattaa
     9181 atgcctcggg ggcacgggca gccttgccag aagcatgtgt aggatcgccc ctcagacagt
     9241 gactttaaaa tatgccctgg ccacaggagc tgaactgaga tgaggagtgc cttttaatcg
     9301 ctgtatcccc gtgtcgctca tgccccctgc atgtgcacaa cacaggtact aaaattaggc
     9361 attcaacatt caaaatacta ctgttttcat aaagttcttc caacccacag acccttttct
     9421 ttataactgt gttttaatag caatatcctg gatttccaat gtcctagctc tttcttctga
     9481 attacctaca accacatcct cactttgacc ttactgcagt gccctgagac atcccaacct
     9541 caaacggaga tacctgggag cgaaggggtt gagtagcttt tctgtcgtcg cctctttgtt
     9601 tgaggagacc tttaactaat aggtaggcct cttggttcct actttgctcc ccagaccaag
     9661 cagacatgcc accatcattc tttgcttttg ctaagaaatg aggacagtac caagttttca
     9721 gggtatacgt ttgctgatgg gtagtgtgtc tgtccacact aggccagtga gcccagaagg
     9781 tcagtgtgcc tccttttacc agtcactttc tcatcactag tttcgtgaac ccctttattt
     9841 ttctcctcgt gagggggatg acattccatc tttccccttt gcctcctgca gcccctcctc
     9901 ctaacccact ttttatggtc aggaggagcg atagtgtcaa atatatcaga aggaagaatt
     9961 gattccgtcg tctttgacga tgtgactgat tagcagagat caggaggagg ctcgtaacag
    10021 cagaaaccca ctgtagcctg gagctttaac agctcttcag ggagctagtt aatgatagaa
    10081 tcactacaga aacacagaca aaggcgcata acgataccac gatatcaaca agtttgtaca
    10141 aaaaagcagg ctggcgccgg aaccgaagtt cctattccga agttcctatt ctctagaaag
    10201 tataggaact tcgaaccctt tcccacacca ccctccacac ttgccccaaa cactgccaac
    10261 tatgtaggag gaaggggttg ggactaacag aagaacccgt tgtggggaag ctgttgggag
    10321 ggtcacttta tgttcttgcc caaggtcagt tgggtggcct gcttctgatg aggtggtccc
    10381 aaggtctggg gtagaaggtg agagggacag gccaccaagg tcagcccccc ccccctatcc
    10441 cataggagcc aggtccctct cctggacagg aagactgaag gggagatgcc agagactcag
    10501 tgaagcctgg ggtaccctat tggagtcctt caaggaaaca aacttggcct caccaggcct
    10561 cagccttggc tcctcctggg aactctactg cccttgggat ccccttgtag ttgtgggtta
    10621 cataggaagg gggacgggat tccccttgac tggctagcct actcttttct tcagtcttct
    10681 ccatctcctc tcacctgtct ctcgaccctt tccctaggat agacttggaa aaagataagg
    10741 ggagaaaaca aatgcaaacg aggccagaaa gattttggct gggcattcct tccgctagct
    10801 tttattggga tcccctagtt tgtgataggc cttttagcta catctgccaa tccatctcat
    10861 tttcacacac acacacacca ctttccttct ggtcagtggg cacatgtcca gcctcaagtt
    10921 tatatcacca cccccaatgc ccaacacttg tatggccttg ggcgggtcat cccccccccc
    10981 acccccagta tctgcaacct caagctagct tgggtgcgtt ggttgtggat aagtagctag
    11041 actccagcaa ccagtaacct ctgccctttc tcctccatga caaccaggtc ccaggtcccg
    11101 aaaaccaaag aagaagaacc ctaacaaaga ggacaagcgg cctcgcacag ccttcactgc
    11161 tgagcagctc cagaggctca aggctgagtt tcagaccaac aggtacctga cagagcagcg
    11221 gcgccagagt ctggcacagg agctcggtac ccggaagatc tggactctag agaattccgc
    11281 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
    11341 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
    11401 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
    11461 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
    11521 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
    11581 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
    11641 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
    11701 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
    11761 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
    11821 gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaaccatgg aagatcccgt
    11881 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    11941 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    12001 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
    12061 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa
    12121 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt
    12181 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt
    12241 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc
    12301 gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc
    12361 tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct
    12421 gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt
    12481 ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac
    12541 tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga
    12601 gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag
    12661 cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt
    12721 cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta
    12781 tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga
    12841 tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt
    12901 gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga
    12961 gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg
    13021 ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt
    13081 ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga
    13141 tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa
    13201 tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga
    13261 cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg
    13321 cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga
    13381 ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga
    13441 gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt
    13501 cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga
    13561 ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg
    13621 cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc
    13681 cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt
    13741 ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa
    13801 cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc
    13861 tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga
    13921 gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc
    13981 agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac
    14041 gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat
    14101 cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg
    14161 gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct
    14221 ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg
    14281 ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac
    14341 acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt
    14401 atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga
    14461 tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc
    14521 gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg
    14581 ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta
    14641 cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca
    14701 ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga
    14761 aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt
    14821 ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg aattccagct
    14881 gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataataat aaccgggcag
    14941 gggggatcta agctctagat aagtaatgat cataatcagc catatcacat ctgtagaggt
    15001 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    15061 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    15121 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    15181 catcaatgta tcttatcatg tctggatccg gaataacttc gtatagcata cattatacga
    15241 agttatgttt ctagcttggc tgcaggtcgt cgaaattcta ccgggtaggg gaggcgcttt
    15301 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
    15361 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
    15421 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
    15481 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
    15541 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
    15601 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
    15661 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
    15721 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
    15781 cgacctgcag cctgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
    15841 caaggtgagg aactaaacca tgggatcggc cattgaacaa gatggattgc acgcaggttc
    15901 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
    15961 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
    16021 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
    16081 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
    16141 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
    16201 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
    16261 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
    16321 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
    16381 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
    16441 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
    16501 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
    16561 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
    16621 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
    16681 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
    16741 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
    16801 cgcggggatc tcatgctgga gttcttcgcc caccccccgg atctaagctc tagataagta
    16861 atgatcataa tcagccatat cacatctgta gaggttttac ttgctttaaa aaacctccca
    16921 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    16981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    17041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    17101 atccgggggt accgcgtcga gaagttccta ttccgaagtt cctattctct agaaagtata
    17161 ggaacttcgt cgagataact tcgtatagca tacattatac gaagttatgt cgagatatct
    17221 agacccagct ttcttgtaca aagtggttga tatctctata gtcgcagtag gcggaggcag
    17281 tgagtgtcac cggaggaagt cgcctcataa tttgtattta cacattgaag taattaccac
    17341 ggacctgagc aaggcgctcc atcatctgac agagttgctt atatagttgc tttccctcat
    17401 atcgggcaga agcgtctaat tctggttgga acctgtcctc tggaagaacg tgattttcag
    17461 ccacggagtt cagctggcct gctgagccca gctttaatct catccctgtc tattcagtca
    17521 ttgtcattta tccattaacc agatgtctgc gttcagatgt acatgacttt gggcttggcc
    17581 ctttaatttt gggggttgtt ctctttcctt tctcctggtg cagtgtctat gaaccggaca
    17641 ggaatgcgtt acggaggaaa gagcgagaaa gaagaagtca agaaacacag caggacagtg
    17701 gcagctttaa ctccggttac tcgctcttca gcgaacccta caaggtagac ggttttcagc
    17761 tgttcacctt ctctttcagc cgccccgaag gccctgagca agagtcaaca agagactcca
    17821 ttttggtttt tggtctgttt agcctaggaa gatcccattg ctcctgtggt gtcacacgca
    17881 gtggggttca cacccctgac gtcaccagta aagtgtgaca ctgagaccca cttgctatga
    17941 cttttatcag gagttcagac tccatctctt tggttccaaa attaatgcat ctgtaatgtt
    18001 ttctgggctc ttctatccca ttcaaaagag gctaggttat atatttggta agtgtggatc
    18061 atgtaaggag attggagggt atcagaatga cttttgtggt ggaggtgcca gctaccaaca
    18121 cacacacaca cacacacaca cacacacaca cacacacact cacactcaca catgctgtgc
    18181 ttcttaccag cactgcagag ttcctctcgc ttccaaaatg aaacactgtc caagagttcg
    18241 cttacttttc ggctgtatgg taacatggta acatggtctg cttagggcgg gagaattttc
    18301 catatttaat cttgtatagc ttctcattta ctgtgattcc tctagcctaa ccccctgtgc
    18361 ttctaacaag aataagtact tgcttgggat ctggaagcaa ccctgttgtg agccaaacct
    18421 aagtcttcaa gaaaagcttt cagtagttct acaaaacaat gatacactta ttctagaaag
    18481 acctaatcta gaccatttaa tgaagtagtt agagtaaatt ttctgatttt caggttacat
    18541 atacattaca cacacacaca cacacacaca cacacacaca cacacacaca cacacacatt
    18601 ttggccgtag cacaaatacc agtaacaact gtttgttaat ataccgtaaa gttatccatt
    18661 acaagtttct agttcttgaa taatgcggag gctaacagct atcttcagag cccataagga
    18721 cttcaatttt gtccctgagt ttttattggg agtgtatcag ccttgtcttt gttacctgac
    18781 aggcacctgt ccactgttgc ctgtatcttg tctccaacat ttccttggat atttttcttt
    18841 ctccagacga acaaggggga tgaactctcc aaccggatcc agaatacatt aggaaattat
    18901 gatgagatga aagacttttt aactgataga tccaaccaga gtcatcttgt tggcgtcccc
    18961 aagccaggtg tccctcagac tcccgtgaac aagatagatg agcattttgg ggcagagtcg
    19021 agagcccagc cccagccctc aaccgtctgc agcactgcgt cctctacacc tgcagctgtc
    19081 cctgtgcagc agggcaagag aggtgccatg ggctggcaga aggctgggca cccaccgtcc
    19141 gatggccaac agagagcagg taaggagggg catgaggcag ttgctttgta cagatctcaa
    19201 agagccacaa tgcaaaacct gttcactgtg tgtggagaac ctcatcctca ccatcactgc
    19261 cttcttcttc caactcagcg caacagggct ctctcaggac cttgcttgga gatggtgttg
    19321 gcagacagca gacaagggcc aagcaagtgt gcaatatgga gacggggctt cagacccagg
    19381 agaggccacc tgcaatggca gccaagcacg gcggcagtgg acactgtgtt cagaactttc
    19441 ccccatccct ggcttcaaaa cccagcctgg tgcagcagaa accaactgcc tacgtgcggc
    19501 caatggatgg ccaggaccaa gctcctgacg aatctcctaa gctgaagtca tccacagaaa
    19561 ctgctgtgca ctgcacagcc tacagagggg tcccggccaa caaaccagag tcagcccgag
    19621 ccaaggccaa gcttgccaag ttcagcatcc ccaagcaggc agaggtgagt tcattgtcat
    19681 tgcctgactt gaaaatgcaa gtcttatgtt tctgcccctc ccatctcccc agcgatgtgg
    19741 taagattcat agcccgcaaa tagaagttgt aaattttgat attttcaaat tcattataat
    19801 agggttgtct tatactgaaa tgcatgagat ggcgcaacgc aattaatgat aacttcgtat
    19861 agcatacatt atacgaagtt atggtctgag ctcgccatca gttcagggat ttcatgaaca
    19921 agatggcatt gactaaggta taaagcagca atataagaca cttcaaaggc cgtatggact
    19981 tcactttcaa ggaggcaaaa tatttccata aaatagttca gaactctgaa cgttttaatc
    20041 aaatttgaaa agttttatga taaacttctt aatggaattt gaatttgtac agtgtataag
    20101 taagaatttg tcaagtatta aatcaatatt aagtctggtc aacattccca tatattaggt
    20161 gtcccaccca ctctataaag cttatgattc caagtcattc ctattcgcca gagtctttac
    20221 ttgggcgctt gtgttaggct gtgtagacgt attctcagaa ggaagtactt cagttcatat
    20281 gcccctgctt tgtgctttga ttttgttcta ccacatttgt gcattgcatt acagaatgga
    20341 gtggatagtg ttgagaattc acatttgcac attgcattac agaatgtgtg gagtggatac
    20401 attgagtgtt gagaatcccc cacaagcccg ccactcgata ggtcgcagga aaggcagcga
    20461 ctagctgctt gaatctgggt ggaaagcaat ccttttagag tagtgtgtct catagtgtct
    20521 cccaaattga taatgattgt caggagtaca tgaggagaaa tcatgaccgt gcatatttgc
    20581 ttttgtcatc tccccgtgat gtagctggaa tggtttttcc ctaacaccat tcttaggaac
    20641 tgctaagttg ttttcccttt gacatgttct tctctaaaga tggagtaatg atgtgactgg
    20701 ggtgttcttc acatcttagt ctgatgtaat aaccaaagga gacaaaggat ttgatgagtc
    20761 ctgttgtgct tttgatgagg ttgagcagag agtttccagg ctatcgtctc agagctctcc
    20821 atgggccagt gggacatgac cctgttacct ttgcaaactg tctgcgttcc aggaggtgtt
    20881 gactgaggtt taattaggaa atgtcgtgat gtgtgaaccg atacttcttt tgattacacg
    20941 ttcataagtt cataatgtat tctagcaatt aggaagcagg aagccttagg aatggggaaa
    21001 gcaaaatgta gcttttccaa gcattctttt taaaagaatt tattttttat tttttaaatt
    21061 ctccctctct ctctctctct ctctctctct ctctctctct ctctctcttt ctctctctct
    21121 ctctctccct ccctccctcc ctccctccct ccctctttct gtgtgtgtat gtgtgtgtgt
    21181 gtgtatgtgt gtgtctgtgt atacctgagt gcaggtactc atgggtatca gagataatgg
    21241 ataccaggga cctgggatta caggtggctg cctgacatgg atgctgagaa taaaacttta
    21301 agatggcaac atgtgctctc aactgctaag cctaggcatc tgtttgtaaa gtctgtaaga
    21361 aatacatgga gaccactata tccaactgat ttcctccaaa ctctaggttt tatcatttct
    21421 atcaaatgat ctctattggc agagaaaatc tagtggccaa cttacacctc cagggatcca
    21481 tttatagttt tccttctagt tgaatcctta ttaaaatgat gttttcatac agcaggaact
    21541 cctgtttatc aggtaactgt tcagttagaa tccagtgagg tagtcagttc tggttaaaag
    21601 caccgagctg tatttttata cccaggagcc ttgcgcagat ccccatacag ttttagggag
    21661 gcattattat cagtagtctg gacattgttg tgctgccctg tgaggatcca agattaggga
    21721 aaacgatgct atgtactagt gcagtgattt cacagccaga gggccgtgcc tgggagaggg
    21781 ggcatctcgg aaaggtcaga gcgacatgta gaaactgctt cattgctcct aagtacagcc
    21841 ttggaaactg acagtctgct gccttctcac accctgtcct gagtgtgcca gtgaggccag
    21901 gcacgcattg gcagaaatgg ggcaactgga gtgttatctg ttgagtcagt tcgagctggt
    21961 gtgagagagg agtcagtctc tgtgttatct aaaagctttg catgttagga aaaacttaac
    22021 ttgactctca agccatgtct gcttatgaag ttcactgcca cagtaagcaa tagactcatt
    22081 caaaatggcg ggcgtctggt cttcacttct gcctgcaaat gacaagattc ttttaaactg
    22141 tttttctgga ggttaacaaa tgcccttctg caggttggta gttttaggct attccagtaa
    22201 aacacacagt aacaacatag aaactgaatt ttatcaacta gatttacttt atggagtgaa
    22261 aaacaatctg acatagcaag caattgaaaa tctcatgtgt gtatgtgcat ctgtgtgtgc
    22321 ctgtgtgtgt gtatgggtgt atctgtgtgt gcctgtgtat atgtatatct gtgtgtatct
    22381 ctgtgtgtct gtgtatattg tgtctttgta catatgtgtg tgagtgtctg taccttaaaa
    22441 aataatccca ttctgaaaat gcattcttag taacacttat ttccagtaac gtatgggaag
    22501 tgttttctag ggaaatatag aggaagtgac gacaagatca cttatattcc tcaagtactt
    22561 gagaatgtta gctatagaac aagacttagt acattgtcaa gcatccagat tctgaaagat
    22621 gtgttagata taaatgagat ttgagagagc agggagggag gaaaggaagg agggaggaag
    22681 ggagggagaa tatgcaagta gctgtctggt attgtgtagg cacattcctg gtcagatggt
    22741 ggctaggtgc agtatcactg agatattaaa aatctacaat gtgatctctg ccttcaggat
    22801 atctatcccc atgaattgaa taaattgctc tcaaacactt agcttttaat ttgcaattta
    22861 tctcacagtc gaaactaatt ttaaaattaa ccttctagtc ccaagaatat tctttcacac
    22921 tctctaccct tgtaaaggga caccgattgt cacttgttta aaaatggaga gacttggatc
    22981 tctttgctca tagttcatca actgtattct atccgccgcc acagagttat ttgttataat
    23041 tctttactct tcaataatgg gtaattagtg cagaaagacc tagtgcggtt tgatgtggaa
    23101 gttaaaagcc tcaagaggat tccttaacca tgcacctgcc tcagctggct tatcccagct
    23161 cagtttgggg tttaatgaaa aacatatttt tgttgttata gctgagtctc ctattcttga
    23221 cgccacccct cattaagtgg ggttccccta gagtgccact tggaaaggta tctgttgtta
    23281 ggcgagaggc aggggactta actaatggct gaagcatgca ttctacaaca tcataggaca
    23341 ggcatggtga atagttttac acacacactt ggggactact aaatagagga gttgatacag
    23401 ttttgtggaa tttgcaattt ttataaaaca tgtaaataac tatataaata tgttaccaag
    23461 gttcttccta agcattggtt acaatgaaac ttaaatgtgc ctaatactaa gatctttcca
    23521 tctctgactc atggggtaat gcaaaattgt ttttaaaata gtaacttttc atttgaaagg
    23581 tactttatat caggctcaaa tttagtacct atttatatgc tgagcaatag gggtgggcac
    23641 atacatcagg agctatgaag tgttatactt cgcttgcctg ctgcttgaag aatctgaaga
    23701 aagcatatga aagtaggacc tgacttggga aggtgtgtca cagactacag agtcagcctt
    23761 ttgggaagtt ttcagtgtct tcagtattaa aaaaacttgt atgtcttact gccatttgct
    23821 gttgatcagt
//