LOCUS       KO-first_condition_ready_30650_MGI:1931881        22185 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Dnajb12 targeting project(s): 30650
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 78794
COMMENT     backbone: L3L4_pD223_DTA_T_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    74..111
                     /note="Transcription Terminator"
     misc_feature    complement(185..438)
                     /note="BGH pA"
     CDS             complement(563..1219)
                     /note="DTA"
     promoter        complement(1233..1745)
                     /note="PGK promoter"
     promoter        1746..1896
                     /note="spectinomycin promoter"
     CDS             1897..2907
                     /note="SpecR"
     misc_feature    3055..3647
                     /note="pUC ori"
     misc_feature    3918..3945
                     /note="rrnB T2"
     misc_feature    4077..4120
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     misc_feature    4275..10071
                     /note="5 arm"
     misc_feature    10099..10123
                     /note="B1 Gateway"
     misc_feature    10136..10183
                     /note="Frt"
     intron          10184..11058
                     /note="mouse En2 intron"
     misc_feature    11056..11058
                     /note="Splice Acceptor"
     exon            11059..11231
                     /note="mouse En2 exon"
     misc_feature    11243..11836
                     /note="ECMV IRES"
     gene            11837..14897
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14936..15179
                     /note="SV40 polyadenylation site"
     misc_feature    15183..15216
                     /note="loxP"
     promoter        15243..15762
                     /note="human beta actin promoter"
     gene            15788..16582
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16782..17023
                     /note="SV40 polyadenylation site"
     misc_feature    17040..17087
                     /note="Frt"
     misc_feature    17094..17127
                     /note="loxP"
     misc_feature    11073..11150
                     /note="FCHK std_gpr"
     misc_feature    17143..17163
                     /note="B2 Gateway"
     misc_feature    10072..17193
                     /note="Synthetic Cassette"
     exon            17563..17743
                     /db_xref="ENSEMBL:ENSMUSE00000290744"
                     /type="targeted"
                     /rank="2"
                     /note="target exon 1 ENSMUSE00000290744"
     misc_feature    17194..17935
                     /note="Critical Region"
     misc_feature    17959..17992
                     /note="Downstream LoxP"
     misc_feature    17936..18015
                     /note="synthetic loxP region"
     exon            18409..18554
                     /db_xref="ENSEMBL:ENSMUSE00000290738"
                     /rank="3"
                     /note="ENSMUSE00000290738"
     exon            20241..20426
                     /db_xref="ENSEMBL:ENSMUSE00001254403"
                     /rank="4"
                     /note="ENSMUSE00001254403"
     exon            20507..20586
                     /db_xref="ENSEMBL:ENSMUSE00001208814"
                     /rank="5"
                     /note="ENSMUSE00001208814"
     exon            21800..21909
                     /db_xref="ENSEMBL:ENSMUSE00001212967"
                     /rank="6"
                     /note="ENSMUSE00001212967"
     misc_feature    18016..22185
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatctata
       61 gtgtcaccta aatccaaaaa aaccgggcca acattggccc ggtttttttc cgtttatctg
      121 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      181 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      241 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      301 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      361 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      421 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      481 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      541 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      601 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      661 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      721 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      781 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      841 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      901 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      961 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
     1021 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1081 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1141 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1201 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1261 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1321 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1381 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1441 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1501 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1561 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1621 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1681 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1741 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1801 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2101 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2881 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2941 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4201 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggagtgtgg agggcagaca ggaaacaaag gagtcttaca tcccaggtca
     4321 gtttggacta catagtgaga acccatctcc gagaaaatag aagttagcag actttgcagg
     4381 gaggcctgag aatacattta ggcctcggtt tccctagcaa agagagaggt ggctgggtga
     4441 ggtgcctgca gctcacgtga cccaagcgtc tgtaaaagcg gctcactgtg cagtctgggt
     4501 aagtcagaga ggccatccaa caaggccgtg ggaagcttgc attccagggc atttggccct
     4561 tcacagtcag tgggagagag aatcctagta ccaacaaggc acagggtgga gctgtccact
     4621 tacatccgtc caccttggtg gtccccagtc cttccattct ctctatttta ttgaatctta
     4681 tttgcttctc taagcagcca ggggcagggt cgaggtatcc tgaggaatta actatggcgg
     4741 caagtgaaca gtaacagatg ctgttggccc cagtcttgtc tgactgccct ggactctccc
     4801 cattctgagc tcttcccttg actccctttg agtgccagag gcctaagaac agaacagctg
     4861 ctagccacac actaagctct gtatacacct cacacctcct cacactgccc ttaggacgga
     4921 agcgcccaga gaagctaggt tgatttatcc aatatgaacc tgctagcaag tgcccttggc
     4981 ccagtctgtg caagtcgggg cctggctcca cactggcgtg gggaagggca ggtcttggca
     5041 gctgtaagtg tcagttgccc taagacttct cttactgagt cctacctttt tgttcgtctt
     5101 tgagacaggg tctctatgta gccctgcctg gcccagaact tgctttgtag gcttggcaat
     5161 gttgaggctc acaaacatcc acctgcatct gcctctcaga cactaggatt agaggtgtgc
     5221 tgtgtgccac cacaccaggc cctttttctt taaataaatt actgttcaaa caacagcgtc
     5281 tcactgtagt gcccaggctg gcctcaaacc ttggacctga gccgtcttca tgcctcagat
     5341 cgcagtagca gagaccatgg gtcacacctt cgaggttcac tgatccagta agcacaccca
     5401 aagctaaggt tagcattatg ctggcctgcc tgttccccaa cccttttgtg ccattgaaca
     5461 ttgtttgtca gccactgtgt cacaggagat gtaatgatga gtcaggatga gactgtcgca
     5521 tgctacaggc cagctggggg catccagacc tgtggggagg cctgcatgcc agcaaccaga
     5581 attcttctgt tttctgctca gtctggaacc ttctgagtgg tgctgagttt gaagaatcca
     5641 ctccttttgg ttctgcagcg tagggaaatc agagtgaccg accagtgcct gagaagcctc
     5701 cagactgagg ctgaggaata tgtgtgcagg cctctgcctc ctgacctgtg cagctgtgtt
     5761 gattgggtca ccggtgctgt tgctcttggg acatgcacag caggctgggc ctggactaga
     5821 gacaacttcc aaagagatca aaacaggcaa cttggttcaa acccccaggg gccaggtcct
     5881 tcctctgctg tcaggaccag ccccttcttg tctgttggtt tcttcctttg cctccactgc
     5941 tgcctggtgt cttgtggtac tgctcagggc tgagatctgg ctatctctgc cttagcctgg
     6001 accacgctag gcacccaggc ccatagcttc cctcccctcc ccagccagct gctcctctgg
     6061 ccagttgctc acccgggttg tgatatttag aacctgatgc cttgccagtc ccagaccacg
     6121 tgcacatgtt atcttagttc atccccacac tatcagtgag gaaactgagg tgccagcagg
     6181 tgtcttagac cagccagggt catggtacca tgctaggaag tggcagacgg gatccggcct
     6241 ctgagaacat gacatgcaga gttcccgttt agagacacag tcggggagaa ccacggagtt
     6301 agaatggaga cctgtgcacc tctgccagga cagtgctgcc atggcgcctt ctccccaagg
     6361 ggaatggcag tggatgatgc cacttcctgt cagaggctgt tgtgatggtt acgtgagcta
     6421 atttttacag agcattgtgg cgttgcctcc ccaacacaca ttgaacactc agtaacagaa
     6481 atggctgtta caagtgggag aatcataatt ctggggccag cctcaactac ttagcaaggc
     6541 acttcctcca aaacagaaga aaagatgatt gctgctaaac tttctttttt taagatttat
     6601 ttatttactt tatatacact gtagctgtct tcagacacat cagaagaggg catcagatcc
     6661 cattacagat ggttttgagc caccatgtgg ttactaggaa ttgaacttag aacctctggg
     6721 agagctgtca atgctcttaa ctgctgagtc atctctccag ccctctacta atcttctttg
     6781 tttgagatag cattctatcc catggcccaa ctctctatgt agcatagact gacctccaat
     6841 tcaaaatcct cctgcctcag catgccaaga actaggatca tggctgagag gtgatggcgc
     6901 atgcctttaa tcccagcaga tggaagggag aagcaggcag atctctgagt tcaaggccag
     6961 cctggtccac agagcaagtt ccaggacagc cagggctaca cagagaaacc ctgtctcaaa
     7021 aatcccgagt gttagggtta tagctgtgca ctacaacacc caaccgctaa tgcttctcca
     7081 agtcctgctt cctcagtcaa agcctcgctc cctgtgctcc tcacggggtc ctgtggtcag
     7141 cttctgtatt tgaattgccc agtctgggga attttctgca ctgaggtagc cgtggccata
     7201 gtgctgtggt cccctgcata ggtgtggcct atatctgtgg tggtggaagg tgtctggtac
     7261 ttggtacact gctgtgacac tggagaagga agtgggatct agcctgagtg gtggcatcat
     7321 ttttccttgg cccataggta tcatgggtcc caacaaggat ctaggtaaca tgtttgccca
     7381 gaggtctgtc aatggctctg gaattgacga gccaggacac cgtagtttgc tagactttgt
     7441 agatgctgag tcaggagctt acatttgaat cgtcacgatc ccgttttagg gtgacagcaa
     7501 acatctcata tagaccccaa tgaagtagat cattcgatat tgccagctag ttgaaaaata
     7561 tagcaatagg gctgaagaca tgtctcagca gttgttaaaa acactggctg ctctcgcaga
     7621 gaacccaggt tcaattccca acacctatat ggtgactcac agctcactgg ctgtaactcc
     7681 agtctcaggg gatctctggg taccaggact gcacatggtg cacagacata tgtgcagaga
     7741 aaatgactgt acaggtgaaa ttaaataaat atgtatatgt aataataatt ggggcttgct
     7801 ggcacacact ggtaattcca atacccagga agtgaaagca gaagcagggt gtttatcaac
     7861 tacgtggaga atttaaaggc tagcctggcc tacattagcc cctgtctaaa ttaaatgtaa
     7921 caatagggct aaggagattg ctccatggta gagcactggc cttgcatgat ttaagtcctg
     7981 tgtgtaatcc ccagcatcac agaggaaaac ctggcaaaga aataatcgcc cagggatcct
     8041 tgtgggccgg gtgggacaga catacttccc catttctcag cgttactaag caaaggctcc
     8101 aagaatcagc cttccctcca ctcccactga atcagacagg ctgctagtgg ccaccacaga
     8161 ggacactgcc ctccctgtat ccaccagagg atgtgtgttg aagtctgaag ggccagcctg
     8221 gagaacaggt ccagcagtgg cccacagcac ccggtttgat cctctgcact gcatggctca
     8281 cgttttacag ctccagtttc aggtacccca ggcatttatt tatcttgtgc atagacattc
     8341 atacaaccaa agccccaata cccatttaaa aagtaaaatt aagaaaaaaa aaatcggaag
     8401 actgggccag gatgttgttc tttggtggag tggctgctca gcctaagtga ggcccgtgtt
     8461 ggttcccagt gaggtgaaaa tacaattgat ttaagatgga tccacgtgag gtggctcgtg
     8521 cttattacaa tcttagcact tcatatgcag aagcaggaag gtcaggatgt tcagggttaa
     8581 ccttcgcttt agagggagtc caaggctagc ctaggcttct tgagatgctg actcaaaaat
     8641 gtccaaaacg taaaatcacc tattcttcag agttgtatgc ttaaagcgtc aagatcggaa
     8701 aagtctgtga agcacaggtc acttgaagtg gggcacgcta gcacagcttg cgcaaggaga
     8761 tggaggcagg gtcaggagct cgaggccagc ctgagctaga catgtaaaga atgaagacct
     8821 gaaatcagac tccactccag ctggcagcca gcaaagccca ggctcactgt gcacaaacac
     8881 agcagtggag acaggagaac ggtggggagg gcattagcta gatcagcagg ccccagctaa
     8941 gcaagcagct ccgcctcaaa actagtgtgg agagagactg gggaaggcac cccacatcga
     9001 cctctggtga tttccacaat gaggaaaaaa actgtggtat tctcagctaa aagaagatgg
     9061 cactattaaa tctaaactag gacagccggg tgtggtggcg caccccttta atcctagcac
     9121 tcgggaggca gaggcaggtg gatttctgag ttcaaggcca gcctggtcta cagagtgagt
     9181 tccaagacag ccagggctac atagagaaac cctgtctcaa aaaaaacaaa acaaaacaaa
     9241 acaaaaacct aagctaggag cactacacag tttataattc tgatatactg tgttaataaa
     9301 caaatattca aactagggaa cgttaaaatt aacattaact tcaatttaat attaagtttg
     9361 cactaataaa aatattcact taattattaa aagtattcta aataacaggc ataaaataga
     9421 agacaacagg aagagtagcc atgaggcctg agttctgggt ggtgtgaggc agcgctgaga
     9481 gaattgctga gagctgagct caacagaggt tcataagaca acaaagagca tagtcaaaca
     9541 aacaagcaca atgaaaaaat accaagacag gaggatggtg aatatgagac cagcctgggc
     9601 tgcatagcag aatcctgttt aaaaaacaac accatcaaaa agaaaagtaa ctgaattcta
     9661 gatcataaac tgctgggttc attagtcaaa ctcgtggggg gcggggggta cactcttact
     9721 gtgtatccct ggctggcctg gatgggatta aaggcatgtg ccaccatgct cggttgtgtg
     9781 tgctcgcccc cctcccctct ccttctcttc ctccctccct ctcttctacc atttggaatc
     9841 tgggagtcag gctcattttc ctgtggagcc atctccccag ggcctcctgt ggtgctttgt
     9901 ggtgcagggt gaggagacag ttattgcagt ctctagcctg tagactgggc tggcccagga
     9961 cggctgtgtt caccctgtgt tcaccgggct agcctgaaac ttaggttcct ttgcttccag
    10021 ggtacttgag tcagaggcgc acaccaccat ccccagttcc tcttttgcag taaggcgcat
    10081 aacgatacca cgatatcaac aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt
    10141 tcctattccg aagttcctat tctctagaaa gtataggaac ttcgaaccct ttcccacacc
    10201 accctccaca cttgccccaa acactgccaa ctatgtagga ggaaggggtt gggactaaca
    10261 gaagaacccg ttgtggggaa gctgttggga gggtcacttt atgttcttgc ccaaggtcag
    10321 ttgggtggcc tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt gagagggaca
    10381 ggccaccaag gtcagccccc cccccctatc ccataggagc caggtccctc tcctggacag
    10441 gaagactgaa ggggagatgc cagagactca gtgaagcctg gggtacccta ttggagtcct
    10501 tcaaggaaac aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg gaactctact
    10561 gcccttggga tccccttgta gttgtgggtt acataggaag ggggacggga ttccccttga
    10621 ctggctagcc tactcttttc ttcagtcttc tccatctcct ctcacctgtc tctcgaccct
    10681 ttccctagga tagacttgga aaaagataag gggagaaaac aaatgcaaac gaggccagaa
    10741 agattttggc tgggcattcc ttccgctagc ttttattggg atcccctagt ttgtgatagg
    10801 ccttttagct acatctgcca atccatctca ttttcacaca cacacacacc actttccttc
    10861 tggtcagtgg gcacatgtcc agcctcaagt ttatatcacc acccccaatg cccaacactt
    10921 gtatggcctt gggcgggtca tccccccccc cacccccagt atctgcaacc tcaagctagc
    10981 ttgggtgcgt tggttgtgga taagtagcta gactccagca accagtaacc tctgcccttt
    11041 ctcctccatg acaaccaggt cccaggtccc gaaaaccaaa gaagaagaac cctaacaaag
    11101 aggacaagcg gcctcgcaca gccttcactg ctgagcagct ccagaggctc aaggctgagt
    11161 ttcagaccaa caggtacctg acagagcagc ggcgccagag tctggcacag gagctcggta
    11221 cccggaagat ctggactcta gagaattccg cccctctccc tccccccccc ctaacgttac
    11281 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
    11341 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
    11401 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
    11461 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
    11521 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
    11581 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
    11641 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
    11701 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
    11761 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
    11821 taagcttgcc acaaccatgg aagatcccgt cgttttacaa cgtcgtgact gggaaaaccc
    11881 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
    11941 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
    12001 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc
    12061 tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat
    12121 ctacaccaac gtgacctatc ccattacggt caatccgccg tttgttccca cggagaatcc
    12181 gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac
    12241 gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt
    12301 cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc
    12361 cggagaaaac cgcctcgcgg tgatggtgct gcgctggagt gacggcagtt atctggaaga
    12421 tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac
    12481 tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca gccgcgctgt
    12541 actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg taacagtttc
    12601 tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg gtgaaattat
    12661 cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa
    12721 actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc acaccgccga
    12781 cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa
    12841 tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc gtcacgagca
    12901 tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata tcctgctgat
    12961 gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta
    13021 cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg aaacccacgg
    13081 catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg
    13141 cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg
    13201 gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca aatctgtcga
    13261 tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca ccgatattat
    13321 ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc
    13381 catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt gcgaatacgc
    13441 ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta
    13501 tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga
    13561 tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg
    13621 ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgctgacgga
    13681 agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac
    13741 cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg tggcgctgga
    13801 tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt
    13861 gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc tcacagtacg
    13921 cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg cctggcagca
    13981 gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca
    14041 tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt ggcaatttaa
    14101 ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac tgctgacgcc
    14161 gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa gtgaagcgac
    14221 ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt accaggccga
    14281 agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga ttacgaccgc
    14341 tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct accggattga
    14401 tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata caccgcatcc
    14461 ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa actggctcgg
    14521 attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg accgctggga
    14581 tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg
    14641 cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc agttcaacat
    14701 cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc tgcacgcgga
    14761 agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg acgactcctg
    14821 gagcccgtca gtatcggcgg aattccagct gagcgccggt cgctaccatt accagttggt
    14881 ctggtgtcaa aaataataat aaccgggcag gggggatcta agctctagat aagtaatgat
    14941 cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct
    15001 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    15061 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    15121 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
    15181 gaataacttc gtatagcata cattatacga agttatgttt aaacggcgcg ccccggaatt
    15241 cgccttctgc aggagcgtac agaacccagg gccctggcac ccgtgcagac cctggcccac
    15301 cccacctggg cgctcagtgc ccaagagatg tccacaccta ggatgtcccg cggtgggtgg
    15361 ggggcccgag agacgggcag gccgggggca ggcctggcca tgcggggccg aaccgggcac
    15421 tgcccagcgt gggcgcgggg gccacggcgc gcgcccccag cccccgggcc cagcacccca
    15481 aggcggccaa cgccaaaact ctccctcctc ctcttcctca atctcgctct cgctcttttt
    15541 ttttttcgca aaaggagggg agagggggta aaaaaatgct gcactgtgcg gcgaagccgg
    15601 tgagtgagcg gcgcggggcc aatcagcgtg cgccgttccg aaagttgcct tttatggctc
    15661 gagcggccgc ggcggcgccc tataaaaccc agcggcgcga cgcgccacca ccgccgagac
    15721 cgcgtccgcc ccgcgagcac agagcctcgc ctttgccgat cctctagagt cgagatccgc
    15781 cgccaccatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
    15841 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
    15901 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
    15961 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
    16021 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
    16081 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
    16141 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
    16201 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
    16261 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
    16321 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
    16381 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
    16441 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    16501 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    16561 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
    16621 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
    16681 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
    16741 ttcttcgccc accccccgga tctaagctct agataagtaa tgatcataat cagccatatc
    16801 acatctgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    16861 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    16921 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    16981 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgggggta ccgcgtcgag
    17041 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcgtc gagataactt
    17101 cgtatagcat acattatacg aagttatgtc gagatatcta gacccagctt tcttgtacaa
    17161 agtggttgat atctctatag tcgcagtagg cgggttttta agaactgagg ctaaggtgcg
    17221 tgtcaccatg gcactttatg tagtggtgct ggctcccggc acgttagcaa acactgccaa
    17281 gtgcttttcc ctataacttg ttaagaaaga aaatgaatgc atatataaat aagcagagta
    17341 tctgaaagga aggaaaccag ttacttcagt ttcatgaatt tgtgagtttg ataatgaaat
    17401 taatattgct tatattttaa aagctataat cttcctattc ctgttcctaa aagaacaggc
    17461 cgaagttgag agggcagcag ccgtgctgga agggtggaga gtggggtacc agggccgtgg
    17521 gcatggcagg cagacccctc agtggcttct tttaccttgc agcactgatc gagtccctca
    17581 accaaaaacc acagtccacc ggtgatcacc ctcaacccac agacactacc cataccacca
    17641 ccaagaaggc aggcgggacc gaaaccccct cagccaacgg cgaggcagga ggaggggaga
    17701 gcgccaaggg ctatacctca gagcaggtgg cagctgtgaa aaggtaggga ccgtgggggg
    17761 gataggatgg gggtgggggg tgtcagggaa gcctctcact tcatccagtg gactcaaagg
    17821 gtcttggaag ttggaggagg tccagcttca tttcccacag ggtgggagcc agggcctcac
    17881 tgcccacccc cacaactgct gacactgcag actggagacc ttgcggaccg acccagagat
    17941 ggcgcaacgc aattaatgat aacttcgtat agcatacatt atacgaagtt atggtctgag
    18001 ctcgccatca gttcatgttt gtcacacaaa gactctgcca tcttgccttc cagattctac
    18061 ccccacccca cccccacccc caacaaagac cttttcagac cagagcctgt ggccaagagg
    18121 cagaggcaga gctgtgtcac caccggggca tggggggcgg gggggaattc agccctgcag
    18181 agtgaggctt tcccaggctt gtgtcaccat ctaggaccac tccagactag accctcccag
    18241 ccgttctttc tcccaccagt tagcctcaca atccttctga ggtcaagcct cctggcaagc
    18301 cagccatcgt gtcctgagta gtcatggtgt ctcctgcagg ccctgccctg agctgcatgc
    18361 ctcacacctc ccctcaggcc ccctgtcaca ctcttctccc tattccaggg tcaagcagtg
    18421 taaagattac tatgagatcc ttggggtaag cagaagtgcc tcagatgagg acctaaagaa
    18481 ggcctaccgc aagctggccc tcaagttcca tccagacaag aaccatgcgc ctggggccac
    18541 tgaagctttc aaaggtaagg cagccctggg ctgtttgagg ttttgtttgt tagtttgtat
    18601 ccctatgtag actgagctgg cctagaactc agagatccct ctgccttcaa atactgggat
    18661 taaaggtgta taccactatg tccagacagc ttagtgtttt tattcctgtc ttaaaataca
    18721 gggtcaagcc tgggcagtgg tggtgtacac ctttaatccc agcacttggg aggcagaggc
    18781 aggcgaattt ctgagttcga ggccaacctg gtctacagag tgagttccag gacagccagg
    18841 gctatacaga gaaactcttg tcttgaaaaa acaaaaaaga aaagtcttta aaagaagaag
    18901 aaaaaaaaat tcagggccga ggcagcttac tcaggaagtg gggctagacg aatgtggtct
    18961 gtagtgtaat tgtacaagac ctgttccctg agccacccct agccatataa actagcagct
    19021 ctcacacagt gcccctgcct cctcctcatt ttccggctta cagtagagga gccagaggcg
    19081 ttccagtggc ttctccaggg tcagcagcac ctaggccatg agcagactct tgacaacagt
    19141 ccctgtaccg aggggcaggg caccgcatac caggagggcg ccttcagaac aggctaccag
    19201 cttcctattt cccacagccc acctcaggcc tgtactaggg acagagagac ttgtgatcat
    19261 acagcccctt gatctgtgcc gtgaatgtgt cacctctggc cagaatccta agtcattctt
    19321 tatgcgttta gagctccatc ttccctctcc ctggtactca tgtgcactgg ctccatgcct
    19381 gccctctggt gctgggcctt gcgttttctt ggtccctggg tactggtccc tctggagagt
    19441 agcgtagtga ctgggagccg tgttgtgcag tactgagtat tagcctgtgg caaagtggag
    19501 aggccttgtg agatggggaa agaagattcc atcctccctc ccaggggctc ccagctctga
    19561 aactagtctg cagcccactg ctgtgagcga ggggagcgta ggggatgcaa gccctgggag
    19621 gctgtagctc tctgccctgg gagctctggt gaggaggccc cagcgccaga tagaaaacag
    19681 cagctgaggc ctgggccctg ggggcagcag actcatcctg gccacacagc agacacacgg
    19741 gtgtctccac caaaaaccgg ggggtggggg tggggttgtg tcttccccgc agtgtctgcc
    19801 gcactcccgt ttacaaccag gacctcgtgg gtcgcaccat gactgcagga ttatattaca
    19861 ctgtctcctt ggcaccccac aagatgagcg tgtgcaaggg gcatttctgt accatcagtg
    19921 gcagagcttg agcaggacac acctgaagca ggtgtatagg ctcatgccca caaccccagt
    19981 gcagaggcag gaggataaag aatggaaggc aacctgggca agttcctgga ccattctggc
    20041 agtttcagtt taggagatgt agcagggacc ctgactcaaa aaaggaaaga gcatcctcac
    20101 agccccgact ttagccttgc ctagaggtgc tctggattgc ctgcccaaca ggcaaaccct
    20161 ctacccctaa gaccccactt ccgctgagac ctgaaagaag cgagctcttg gctgtgctaa
    20221 ggctgtttct ccccttgcag ccattggcac agcgtatgcc gtactcagca acccagagaa
    20281 aaggaaacag tacgaccagt ttggtgatga caagagccag gctgcccggc acggccacag
    20341 ccacggggac ttccaccgag gctttgaggc tgacatctcc ccagaagacc tctttaacat
    20401 gttctttggt ggcggctttc cttccagtaa gtccacagcc agttctcctt agcggtggtt
    20461 ctggggtctg actgctgcag cccctgactc tatctccatg tttcaggtaa cgttcatgtc
    20521 tacagcaatg gccgaatgcg gtacacctac cagcagaggc aagaccgcag agacaaccag
    20581 ggtgatgtga gttgccagag gctgagtaac actccagccc cccaccccat tccacccccc
    20641 agggcatggg ccccagagga ggagggagtc ttgatccact taagaccccc ccagcattct
    20701 taggccaagt agccatcttt gctccttccc agtgccattc tgcctaggga caccagaggg
    20761 agccacactc cgtgttgcgt gatgctttgg cttctgttgg gggcataaaa caaagcctca
    20821 cctccaggca acgcatagtg ggaacagctt ctatggagtc tatttgttct taagactgac
    20881 gtaacatttt ctgagtgatg gaaagttaaa acatgatgag aggaagaaag aggaccgtgt
    20941 gggtattcag ccttctagga gagtgaggaa aagctgtata gccaggcagt gtgcttcaga
    21001 cacatttggg atgccacgga tcaacacagc cagtgaagag cattgaacag ggggacacgt
    21061 ccttacgcat gcctgccctt tttcctcctt ctccctccct ccctccctct cttctattct
    21121 ttttcttccc cttcctcctt tcttcctctc tgtctccttg gatttaccct gcagttgaaa
    21181 acaaccttga agccctactt ccaccttcca aatactggga ttatgcctca tcgacctagc
    21241 ataacgccca gcacatccgt tttgttaggg gtgggtttgg ctttggattt tgaggcgcgg
    21301 tctcactgta tagctctggc tggcctagaa cttgctgtac agactagctg gtctggaact
    21361 gcaactccca acccttctcc cgtccctgcc tcctgagtgc tgggattagt gtatctttac
    21421 atctaacgta cagaagagtt taaagtcaga ttcatgtgta atgcctttat ttctggctgt
    21481 cctgtctttg ttctaagaat cacacccaag gccttgtggg tactagaaca gttccatgcc
    21541 acagactgca tcccagccct ttaattagat gctttgctgt ctgcagaaca tttgtgtggc
    21601 tcagaattca gaaagctgtg gatggtgagg gtctgtgtcc ccggcctgcc acttgctctc
    21661 acactcacac gtgtgctcca aagcggttgc tttgtcctct gggaaagctg tcagagggaa
    21721 gggaggagag ggggctgtcc caggcaggcg acacgacact gttcagggct gtgaccactg
    21781 ctcttgctct tcccctcagg gcgggctagg agtgttcgtg cagctgatgc ccatcctcat
    21841 cctcatcctc gtgtctgcgc tcagccagct catggtgtcc agtcctccct acagcctgag
    21901 cccgagaccg tgagtgcacg ccccgggcag ggccgggctg ggcccctcgc catcttgcat
    21961 accgtccccg ggccgggccc cttagacttt tgaggatcat aggcctcgca gagaatgcag
    22021 acgaagctcc agccctttcc ttaaggcagc acacatgcac tgtcctacgc gatttcagaa
    22081 gcgtgactcc ccgccactgt ccccaccttt gctgacgctg agatgtcctc tgctcttgaa
    22141 gtctgtttga agccctaaga aagaaagaac agggtatagt caatc
//