LOCUS       KO-first_condition_ready_39466_MGI:1926142        21486 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Crispld2 targeting project(s): 39466
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 43718
COMMENT     backbone: L4L3_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(4153..4190)
                     /note="Transcription Terminator"
     misc_feature    3826..4079
                     /note="BGH pA"
     CDS             3045..3701
                     /note="DTA"
     promoter        2519..3031
                     /note="PGK promoter"
     promoter        complement(2368..2518)
                     /note="spectinomycin promoter"
     CDS             complement(1357..2367)
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     GK"
                     /note="SpecR"
     misc_feature    complement(617..1209)
                     /note="pUC ori"
     misc_feature    complement(319..346)
                     /note="rrnB T2"
     misc_feature    complement(144..187)
                     /note="rrnB T1"
     misc_feature    4216..4236
                     /note="B3 Gateway"
     misc_feature    4242..4249
                     /note="AsiSI"
     misc_feature    1..4274
                     /note="Synthetic Backbone"
     exon            5568..5885
                     /db_xref="ENSEMBL:ENSMUSE00000304524"
                     /rank="2"
                     /note="ENSMUSE00000304524"
     misc_feature    4275..8706
                     /note="5 arm"
     misc_feature    8734..8758
                     /note="B1 Gateway"
     misc_feature    8771..8818
                     /note="Frt"
     intron          8819..9693
                     /note="mouse En2 intron"
     misc_feature    9691..9693
                     /note="Splice Acceptor"
     exon            9694..9866
                     /note="mouse En2 exon"
     misc_feature    9878..10471
                     /note="ECMV IRES"
     gene            10472..13532
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    13571..13814
                     /note="SV40 polyadenylation site"
     misc_feature    13818..13851
                     /note="loxP"
     promoter        13878..14397
                     /note="human beta actin promoter"
     gene            14423..15217
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    15417..15658
                     /note="SV40 polyadenylation site"
     misc_feature    15675..15722
                     /note="Frt"
     misc_feature    15729..15762
                     /note="loxP"
     misc_feature    9708..9785
                     /note="FCHK std_gpr"
     misc_feature    15778..15798
                     /note="B2 Gateway"
     misc_feature    8707..15828
                     /note="Synthetic Cassette"
     exon            16181..16299
                     /db_xref="ENSEMBL:ENSMUSE00001246326"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00001246326"
     misc_feature    15829..16451
                     /note="Critical Region"
     misc_feature    16475..16508
                     /note="Downstream LoxP"
     misc_feature    16452..16531
                     /note="synthetic loxP region"
     exon            17413..17545
                     /db_xref="ENSEMBL:ENSMUSE00001249968"
                     /rank="4"
                     /note="ENSMUSE00001249968"
     exon            17805..17920
                     /db_xref="ENSEMBL:ENSMUSE00000213428"
                     /rank="5"
                     /note="ENSMUSE00000213428"
     exon            20992..21092
                     /db_xref="ENSEMBL:ENSMUSE00000213416"
                     /rank="6"
                     /note="ENSMUSE00000213416"
     misc_feature    16532..21486
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatttaag
       61 actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag
      121 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      181 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      241 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1321 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1381 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2341 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2401 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2461 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagct gtttatcgaa
     2521 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2581 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2641 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2701 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2761 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     2821 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     2881 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     2941 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     3001 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     3061 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     3121 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     3181 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     3241 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     3301 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     3361 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     3421 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     3481 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     3541 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     3601 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     3661 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3721 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     3781 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     3841 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3901 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3961 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4021 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4081 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgagttt
     4141 aaacagataa acggaaaaaa accgggccaa tgttggcccg gtttttttgg atttaggtga
     4201 cactatagat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4261 aatttcacac aggatatata tacaattctg tgtgtgttgt ctctttctgt ctatgctttt
     4321 ccagctctgt gaatggaagg tggccaggtg gcccactgct ccgtgtatca agcatacacg
     4381 cactatcatc ttctactggt ttttaaactt accaccatgc tgtacttatt ggctcctttt
     4441 accccctata tagttctcta ccccatttcc tgccaccagt cgatccaccg ctcctcctac
     4501 tgactgcact ctgattctgt ctgtttctgg gtttttatct ggttggccat ggtaaggtca
     4561 catgacagtt aatgctttgg atcctgccct ttgagctaga caagcacgga aaaacacagg
     4621 tggggcattg ccatgatccc agggtggaga ggcagtgaca tgttttggtg tgtcactttg
     4681 acctgactct gcagctcggg atgtgtgttt atggtgatat gaccgaccca agagagcttg
     4741 ggcaccctga atgttctgca ttcttggtgt gcttcttggc tggggccatt gcacacagtg
     4801 ggccctccgt ggtctcctgg gctccgcccc cgaggcttcc acccttggag cagggcaggg
     4861 tttgagtctg gcataggaga gagcagctga gccccgcctc tctatgttgt gcctttggtc
     4921 ttacacaggc ttggttggga ccctggaggg tttccagggg cgtgccctgg ctaagcatcc
     4981 cagagactta attagtgagt cctgatctac agagtcactg cttgacctgc ctcccagcat
     5041 ccttcccaga aggcaggaca gtatgcaaga acctcagagc cagacctgat tgtctggctt
     5101 ttgggtgtcc ctgtccctga attgggtgtg ttggtattca caggtcagaa tggaagcctt
     5161 tccagagagt atcatgaaac agacagtgaa cagcccgcta gccctaagaa cactgttcta
     5221 gccccagctc ctgtggacag ctaccttaac ccatgttgtc ctgtttgtta gaatgggagc
     5281 tataagatca tacttcaggg taatagagag cattctggct atgcctgcct cctgggacag
     5341 gaaataactc tctagaactc tgcctcatct tccatcctct cccttcctct gtccaccttg
     5401 cccctggaac tcccaagccc acatgacagt tacatccctg gagtttctgt gtgagcaaag
     5461 acaaggggca actttgacta atgggacaga gccttccctg ttctgatgag gtggtatggc
     5521 tgacctggtt ctaatttgct gtatcccatt tcctcttttc cttccagaac cttccggtgc
     5581 ccagccctac ttcaggagcc caggctgccc ccacccctct tagttgtggc tgaaggcgtg
     5641 aagccatgag ctgtcttctg aacaatatgg tcctgatggg gctggctctg ctggtctgtg
     5701 gagtacaggc ctttttcctt cccaacacca cgagcctgga gaagctgctg agcaaatacc
     5761 agcatgcaga gccacactca cgcgtgcgca gggccatccc catgtcagac cgccaagaga
     5821 tcctcatgct acacaacaag ctgcgaggcc aggtgtatcc ccctgcctcc aacatggaac
     5881 acatggtgag catcccaccg gggtgatggt gggcccaggg gaggggcagt gtcttacgat
     5941 ttttttttga gaggtctcac aaagtcctgg ctatccttga actcgctatg tagctggggt
     6001 tgactcaact tcgaagtgac ccatctgcat ccagcctcct aagcgttggc gttaccaatg
     6061 tgcctttctg acagcttagg ctgactttaa acccgatctg tagtcaagga tggccttgaa
     6121 cttcgggtct tctgctccat ctcccaagtg ctaggactac agatgtccac caccgtgccc
     6181 agtttatgca gcgctggaga tggagcccag ggccgtgtgc atgcgaagca agcattctgc
     6241 caactgagct gtactctccg gactcagcta caggctctct tctaactgag atgcactctg
     6301 cccaccggca ccccacttca cacctccttt ctcttctaat ccctcctttc tctctctccc
     6361 tcagtggacc caaggccagg cttctctctc tgttcagccc tgtttctgtc cgtgtccccc
     6421 atgtcacagg ctacctcctc tctgaagctg ccattgactt ctgacttagg gtttctattc
     6481 cttcacaaac atcatgatca agaagcaggt gggggaggaa agagtttatt cagcttccac
     6541 ttccacttat tcagctgttc atcaccaaag gaagtcagga ccggaactca agcaggtcag
     6601 gaagcaggag ctgatgcaga ggccatggag ggatgttcct tactggcttg cttcccctgg
     6661 cttgctcagt tttcttatag aacccaagac caccagccca gggatggtac caactacaag
     6721 gggccctcca acccttgatc actaattgag agaacgcctt acagctggat ctcatggagg
     6781 catttcctca agggaggctc ctttgtctgt cataactcca gcttgtgtca agttgacaca
     6841 caaaaccagc cagtacaact ttctcccaga gaccaccctg ggctaatggg ttcttcatgg
     6901 tctcaagggt ctagaatgag tggccagtta tgtgggcccc aagcaaggct gctctggggt
     6961 agcacagtat aaacctgaat cttttccctc tggaaatctg tgcacccttc caagatgtca
     7021 gcccatggag tgggtgtgtg cttctttttg cagggcatgg ttagaaacat aaaaactcat
     7081 ggcagagttc ttgggtaggc tcctgaggta cctaggcagt ctcctctttt agctgctgtc
     7141 tcatgcctgg tctttgccaa ggatggcaga agccagagag gccagctctc ctgagctctt
     7201 gctacaagct ggggtagcca agtgaagtgg ccatttgtcc ctgagttcca gggtggaaag
     7261 attccccttg gctggctttc cagaactgag gtgaggatcg gggtgaggat gaagctgacc
     7321 acacaccaca gggtcactcc ttgcccctgg gggtgaccct gcctcagtag attgtaaacc
     7381 ttcagagact tgatgctcac tgcttcccag atactgccca ggtggccggg taccactcac
     7441 acaggagtgg gacctctagg gacggctcag agaggccatg ttgtcaatgg tagcttcaga
     7501 ctgtccttct atcccctttg ccaaggatga ggcagctcct gctgggggac catgtcccag
     7561 cagccacccc tgggcaggtg ggcagtgtgg tgcatcttcc cagcagagac accatggaga
     7621 accaggttgg gggtgggagc gtgaggacct gaggggccct gctgggactc cgaggaacag
     7681 gaagtattta ttctggtctg cagatgggaa actagactca ggctaatctg gaagggtcat
     7741 ggccctgaca tacatagtga ggggataaag atggggaact ctctagagct agcctctgcg
     7801 ccaggtctct tggcccgcca actctctcgg ctcgccaact ctcccgccct gctaggccat
     7861 atggcttcca ccaggccatt tcttggatac tccccccacc ccaagttcct ctcgctttca
     7921 tgcaacaccc cacacacccc tgattagcca tctcaacacc taattagctg gtagaatcaa
     7981 gactcttact gtttaattag ccaatcagat gtatgtatca ataataacac aatttacaag
     8041 atgacgatac aataatctct gagccaaatg ataatgatta agctttaaca caattattct
     8101 aaccttttga taacaccact tgtcagcctc cattctggtt ctctctcttc tcagccctct
     8161 ctctcatctc cccaactact cgctccgcct cctttctcct gtccaatcac gagcctccct
     8221 cccactgccc taatgtgatt ggacagggaa aaatcctacc tttcaagcct tctgtctgcg
     8281 gtgaggttta gcttattggc aagttctgtg gagtagttga gcacgcggtg taggcatgca
     8341 catggggggt ggtggtggtg gtgggagggg atcttcacac aaagagaaga tggatgaaac
     8401 ctcgtggggc tgctgaccag aggagcccat gccccccgct gccgcacccc ctgatagcag
     8461 gcttctagag gctgaggaga cccagaaagc ccatgaggga ctcaaggtgg ctgcagaagc
     8521 taacggctga ggtggtcggg agctggagta ggaagattga gtccgtgcgc agggaagggc
     8581 gcgagggcac ggaatgcaga aaccttgcta ctttccacag ctccttccct aaaaaggcaa
     8641 caagttgggg gctccccagg ggtctgctgg tgcttgaatt tctgattttt tccctggcaa
     8701 catatcaagg cgcataacga taccacgata tcaacaagtt tgtacaaaaa agcaggctgg
     8761 cgccggaacc gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcga
     8821 accctttccc acaccaccct ccacacttgc cccaaacact gccaactatg taggaggaag
     8881 gggttgggac taacagaaga acccgttgtg gggaagctgt tgggagggtc actttatgtt
     8941 cttgcccaag gtcagttggg tggcctgctt ctgatgaggt ggtcccaagg tctggggtag
     9001 aaggtgagag ggacaggcca ccaaggtcag cccccccccc ctatcccata ggagccaggt
     9061 ccctctcctg gacaggaaga ctgaagggga gatgccagag actcagtgaa gcctggggta
     9121 ccctattgga gtccttcaag gaaacaaact tggcctcacc aggcctcagc cttggctcct
     9181 cctgggaact ctactgccct tgggatcccc ttgtagttgt gggttacata ggaaggggga
     9241 cgggattccc cttgactggc tagcctactc ttttcttcag tcttctccat ctcctctcac
     9301 ctgtctctcg accctttccc taggatagac ttggaaaaag ataaggggag aaaacaaatg
     9361 caaacgaggc cagaaagatt ttggctgggc attccttccg ctagctttta ttgggatccc
     9421 ctagtttgtg ataggccttt tagctacatc tgccaatcca tctcattttc acacacacac
     9481 acaccacttt ccttctggtc agtgggcaca tgtccagcct caagtttata tcaccacccc
     9541 caatgcccaa cacttgtatg gccttgggcg ggtcatcccc ccccccaccc ccagtatctg
     9601 caacctcaag ctagcttggg tgcgttggtt gtggataagt agctagactc cagcaaccag
     9661 taacctctgc cctttctcct ccatgacaac caggtcccag gtcccgaaaa ccaaagaaga
     9721 agaaccctaa caaagaggac aagcggcctc gcacagcctt cactgctgag cagctccaga
     9781 ggctcaaggc tgagtttcag accaacaggt acctgacaga gcagcggcgc cagagtctgg
     9841 cacaggagct cggtacccgg aagatctgga ctctagagaa ttccgcccct ctccctcccc
     9901 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     9961 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
    10021 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
    10081 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
    10141 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
    10201 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
    10261 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
    10321 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
    10381 tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
    10441 tgaaaaacac gatgataagc ttgccacaac catggaagat cccgtcgttt tacaacgtcg
    10501 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
    10561 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
    10621 gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct
    10681 ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg
    10741 ttacgatgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc cgccgtttgt
    10801 tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct
    10861 acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg
    10921 caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag
    10981 cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg
    11041 cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt
    11101 gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga
    11161 tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct
    11221 acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt
    11281 cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa
    11341 cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga
    11401 actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga
    11461 ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt
    11521 taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca
    11581 ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa
    11641 ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa
    11701 tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc
    11761 ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat
    11821 catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg
    11881 gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac
    11941 ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc
    12001 tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat
    12061 cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca
    12121 ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc
    12181 gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga
    12241 tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca
    12301 tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca
    12361 aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tcctgcactg
    12421 gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc
    12481 acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact
    12541 ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat
    12601 cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc
    12661 ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa
    12721 gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa
    12781 acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg
    12841 cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg
    12901 ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt
    12961 gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa
    13021 aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag
    13081 cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg
    13141 ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg
    13201 ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga
    13261 aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga
    13321 cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca
    13381 tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg
    13441 tggcgacgac tcctggagcc cgtcagtatc ggcggaattc cagctgagcg ccggtcgcta
    13501 ccattaccag ttggtctggt gtcaaaaata ataataaccg ggcagggggg atctaagctc
    13561 tagataagta atgatcataa tcagccatat cacatctgta gaggttttac ttgctttaaa
    13621 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    13681 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    13741 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    13801 tcatgtctgg atccggaata acttcgtata gcatacatta tacgaagtta tgtttaaacg
    13861 gcgcgccccg gaattcgcct tctgcaggag cgtacagaac ccagggccct ggcacccgtg
    13921 cagaccctgg cccaccccac ctgggcgctc agtgcccaag agatgtccac acctaggatg
    13981 tcccgcggtg ggtggggggc ccgagagacg ggcaggccgg gggcaggcct ggccatgcgg
    14041 ggccgaaccg ggcactgccc agcgtgggcg cgggggccac ggcgcgcgcc cccagccccc
    14101 gggcccagca ccccaaggcg gccaacgcca aaactctccc tcctcctctt cctcaatctc
    14161 gctctcgctc tttttttttt tcgcaaaagg aggggagagg gggtaaaaaa atgctgcact
    14221 gtgcggcgaa gccggtgagt gagcggcgcg gggccaatca gcgtgcgccg ttccgaaagt
    14281 tgccttttat ggctcgagcg gccgcggcgg cgccctataa aacccagcgg cgcgacgcgc
    14341 caccaccgcc gagaccgcgt ccgccccgcg agcacagagc ctcgcctttg ccgatcctct
    14401 agagtcgaga tccgccgcca ccatgattga acaagatgga ttgcacgcag gttctccggc
    14461 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
    14521 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
    14581 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
    14641 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
    14701 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
    14761 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
    14821 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
    14881 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
    14941 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
    15001 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
    15061 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
    15121 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
    15181 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
    15241 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
    15301 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    15361 gatctcatgc tggagttctt cgcccacccc ccggatctaa gctctagata agtaatgatc
    15421 ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    15481 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    15541 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    15601 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgg
    15661 gggtaccgcg tcgagaagtt cctattccga agttcctatt ctctagaaag tataggaact
    15721 tcgtcgagat aacttcgtat agcatacatt atacgaagtt atgtcgagat atctagaccc
    15781 agctttcttg tacaaagtgg ttgatatctc tatagtcgca gtaggcggtt gtgggcgtct
    15841 ggcctggctt ctcaaagcct ctgaccttta ttctgtaaga tgaaataaga ccaggaatga
    15901 ggcaggaagg cggggagaga cttgggtgca tccagggctg gcactggggc tgtgagtggg
    15961 ctcttggccc ctaagaatgg cctgtgagtg accaagatct tacctggcct ctcgggtgtc
    16021 tgactgctgt catttggcat gccccgcccg atcccctagt tgtgtgctcg ccggggcttc
    16081 tcacaggtta gaggcacagc ctgtgctctg acagtccctg gggctgcggt ttctgcttcg
    16141 ccctcctgac ggtcagctgc catctctctc tgccctacag acttgggatg aggagctaga
    16201 gagatctgca gcagcgtggg cacatagatg cctgtgggag cacgggcccg ccggccttct
    16261 gaggtccatc gggcagaacc tggctgtgca ctggggcagg taagagcagc cagcaccccc
    16321 gagctgcagc gtcccctacc tgagtgtgag ttggctgttg gccacagagg acttggtgtt
    16381 cccgcgggtc atcaaggtgt gtgtgtgggg gggggggcgg tagagtacgc atctgatagg
    16441 tgtgaggctc tgagatggcg caacgcaatt aatgataact tcgtatagca tacattatac
    16501 gaagttatgg tctgagctcg ccatcagttc acatttttac aaaattccaa ctgcagccca
    16561 gactgccgcg tccccccacc cccgcgcccc gctgacttcc cctttccttc tatgggcccc
    16621 cttccttcct gcataatggt gtgtagcaag catagcattc cttgggtgaa gatggccttt
    16681 ctgtccccac agctttggtt tggtgcttga ttttattctg aggccctccg gtcatgcccc
    16741 agggatgctg ggacagggat gaggttagag gctcagactg tccctgctgt gataggtagg
    16801 gtcctcccct aaggcatgct ggtgtgggtt cagtatctgt aggagtgaca gccaggcccc
    16861 cttctgcccc agctcctcgt ggcacgtgcc tctgaggggc tgaccagggt gggaggccag
    16921 tgtcccggga cttgctgacc acagctgctg atgctggtcc cagctggtaa ttgttacaag
    16981 acctcaaact gcccggctga ccgcaatgct gtcactaagg gtcactctgc ctgagacctc
    17041 attctcttcg aaccactctg tttacacttg ctgtgggttc atagacaagt tcttattttt
    17101 gcttcctgat tgaaacaaaa cagaacaggg agtgtggagc ggctcacacc tggaacccga
    17161 gccctcagga tggggaggca ggggagtcgt gagttcaagg ccatcctggg gcaatggctg
    17221 caggtctatg gtcccaccac agtgtcagag ctgtgtgtca ctcttcagac actcagtttg
    17281 taagatatgt ctacgatgtc tacttttcat ccctctgtct ccttgcaaag tacttctgct
    17341 tggccctgct tgactgattg attctctctc tccctctctc tctctctccc tctcccactt
    17401 ctatccccat aggtaccgct ctcctgggtt ccatgtgcag tcatggtacg acgaggtgaa
    17461 ggattacacc tacccatacc cccacgagtg cactccacgg tgccgggagc ggtgctcagg
    17521 ccccatgtgc acccactaca cacaggtaat gcgggcgctg aactctactc agcctgggcc
    17581 ccagacctcg gggttgtgtt tggaaatggg agtgcatgaa gcctttgctt taattccgtt
    17641 tgtctcagag cacactggtt gggtcccact gtttgctatc aatgagctca ctgaggagac
    17701 acctggagta ggctctggaa actttgttgc tgagattttt caccagtgcc ctacagagtc
    17761 accctgaggc acccttgagc ttcaacacag tgctgtcatt tcagatggtc tgggccacca
    17821 ccaacaagat cggctgtgcc gtgcacacct gtcggaacat gaacgtctgg ggagacactt
    17881 gggagaatgc cgtgtatctc gtctgcaatt attctcccaa gtaaggctac gttgtctcgg
    17941 tcacacaagc caagctgggg tatgcttgtt agccatggca gaacgagcta aaactggcct
    18001 caagagtgaa gtcttagtta cctcactaac aaagagtcta gaaatggctg catttaggtg
    18061 agcaaatgct ctggagagtc agtcagcaac gacttgggcc atcttaaggt cctcatggcc
    18121 ccaacgtggc tgctgagttc cagcgctctg tcttcacata caattccact taacgtagta
    18181 gaattatagg aatgagtcgt ctcctctgga attgccccac aaaatgcttt tgaccagagc
    18241 ctagatcaat catagacgag ggggaaccac aattgctcca actggttttc tgagtcatct
    18301 cctaaaaccg ctgtacaagc aagggtagcc gttgtctggc aggagggagg accagagtgg
    18361 agaatgaggt gaggagagag tggaaaaaga ggagaggggc aggagacaga gagagagaga
    18421 gacagacaga cagacagaca gacagacttc atcatcagtg ccattttctc tgaagtggag
    18481 ccacagattg agtcctctaa tcagaaacct aactcctggg gccagtgata tggctcagca
    18541 ggtaaagggg cttgcctcca agcctgatga tctgagttcg attcctagga cccacatgac
    18601 ctcccaccag attgggtctc tcaggttctc cttcccctcc taaacaggcc acaccaggtc
    18661 tgtccagtgt agctgccctc ttcacagagc aaccccagtc acagggcgtc attcacccaa
    18721 cggctcactg cacagatatg tacagagcgc agagctactt ccccgtttgc agcctgtggt
    18781 ttgaacaacc atgctggcca ctatcccggc acgggcacag gcactgtgct gtgctgtgct
    18841 gtgctgtgct gtgctgtgct gtctgacatg cttacttata agaggaataa agtaattcag
    18901 gtcagaggag ggggagtagg gtgggggtgg ggaccatggc tttggggtgg cagctgagca
    18961 gccaccttcc tgcagagtct gcttctgagg tgaggcgatt cctagctcag ctgaaggtgc
    19021 aaagtgagag acaagttgga ttaaattttt atgttaaaaa aaaaatgtgc cgggtgtggt
    19081 ggcacacacc tttaacgcca gcactcagga ggcagaggct ggcggatttc tgacttcgag
    19141 gccagctggt ctacaaagtg agttccagga cagccagggc tacagagaaa ccctgtctcg
    19201 aaaaacaaaa acaaaaacaa aaacaaaaac caaaaaaaaa aaaagctggc agctctaatc
    19261 agaaacctaa actctcaggg ccagtgagaa ggctcagcag gtaaaggagc ttgcctccaa
    19321 atctgatgac ctaagtttga ttcccaggac ccacgtagca ggaagtaaaa accaacttcc
    19381 acatggatat acgcatacac attaaattaa aaaatgcaaa aagatttttt tttaacagaa
    19441 ataaaacctt tcccttccct gctgctgttc tctccccttt cttggtaaag gcaccgcagg
    19501 gtcagggtct tctgtcgaac cagcctagga cctagagtaa actcttcagg ctacatcctg
    19561 tgtgggagga cagaatgaag gcccgggagg cactgagtct agggaaatgt cccctggctt
    19621 gcagttacca atcttccttc tgcactccca ctcggctgtg ggagagggca gagagtgagc
    19681 atgcggggct gtggaagagg gcagagagtg agcatgcgcg gctgtgggag caagcagaga
    19741 gtgagcatgc gctttggaac tcctctcgtg aaggttctgg tcctcctgga gcagggcccc
    19801 cctcttggga tcgcattaac attactttcc taaaggccca tactgacatg cggcattcat
    19861 tgcgaatgag ggccggcact gcctgagacc cagccacagt cagtttgttt gctacttagt
    19921 tacttttgac tgctgtataa tattccacac ttccatggct ttctctttaa gaggcattcc
    19981 caggctctta gctgcccggg acaccatcat ggaaccaggc aggagacaat gcacaagagg
    20041 ctgggaatag agcaagatgt acctggctct tgtccctatg acctcacgtg tgcagacagt
    20101 gccatccgtg ggatagtcca aggtttggga gcctagccat ctgtctagct agaaagtcca
    20161 gccagctcag tgaacattgg tttctgccct tatggacctg gaagcctgga aatgtggcct
    20221 gtctttaaag aaacagagtc cactgtgcag agaactgggc gggggaacag aaccaaaccc
    20281 ctatgggcaa ggggaccacc acgctctccc acgaacagct atgcctgatg tcccactgag
    20341 gttcacctca gtgcccccta ctgtgtgtcc ccagcccaaa tacttgaggg cttagaactg
    20401 tggggcccac agccacatcc agagattgtt ccaactgtcc aggtgggcct atagagagga
    20461 gggtgttctg cgacggtgca gaggagagaa cgagaccccc ctcccccagt gcgggccacc
    20521 tccctccctg gctcagggtt gtcatcaccc tttggccatt ggagtggacc acaggataca
    20581 cagaggtgtc tgtgcagcat ctcgatggct ctagcgggag ctcctgcctc tttagaaagc
    20641 caccacaggc caccctggcg ggcctctttg ctttatacat actatcaaga acattgaccg
    20701 agggttgccc tggtgatcat ccactgaatg aataattagc caaagctacg ggttggggac
    20761 aatggctgag gtcacttaga tggatctgag gccacatatg tcacccaggg agccatttct
    20821 tacacagtgc tcagacaggc tctcatcatg gtgtccagag ccccctggtt gactggtact
    20881 ggagggggtc gacatctgcc ttttctctga cccataccta ccagggactc tggggtcccc
    20941 tggtggacct aaaggatgac ctccttcatg gcgtttctgt tctgtctcca ggggaaactg
    21001 gattggcgag gccccctaca agcatggccg tccctgctct gagtgtccat ccagctacgg
    21061 aggaggctgc ctgaacaatc tttgccaccg aggtaggtgg cacgagggct gtggcctgag
    21121 ccacaggttg tgatggctga ggtagaaatg aagtcagatc cagacccctg cagccacccc
    21181 tggatcccct ctaccctctg cactcagccc gtgcccccct gcagacacca ctgtcctgag
    21241 atgcccctca cgggcaactc tttggcctgg tcccagtgga cagtggacat ggccttgacc
    21301 tgaacccctc ccccccaacc ttccttagca cgtgcagtct gcttaaaaca gatgtcactt
    21361 aagtcagaag tggtagctca cacctgtcat cccagcactc gggtggttga ggcaggagga
    21421 ttgccgtgaa tgcgagatca gcataggcta tttagtaaga ctctgtttca ctcccccaac
    21481 aaaacc
//