LOCUS       KO-first_condition_ready_32278:119943_MGI:1924104        24077 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Gper1 targeting project(s): 32278:119943
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Bact_P
COMMENT     design_id: 220789
COMMENT     backbone: R3R4_pBR_DTA+_Bsd_amp
FEATURES             Location/Qualifiers
     misc_feature    29..152
                     /note="R4 gateway site"
     promoter        399..911
                     /note="PGK promoter"
     CDS             925..1581
                     /note="DTA"
     misc_feature    1706..1959
                     /note="BGH pA"
     promoter        2034..2100
                     /note="EM7 promoter"
     CDS             2101..2499
                     /note="BsdR"
     CDS             complement(4193..5053)
                     /note="AmpR"
     misc_feature    5272..5395
                     /note="R3 gateway site"
     misc_feature    5401..5408
                     /note="AsiSI"
     misc_feature    1..5433
                     /note="Synthetic Backbone"
     exon            8713..8916
                     /db_xref="ENSEMBL:ENSMUSE00000300256"
                     /rank="1"
                     /note="ENSMUSE00000300256"
     exon            9223..9434
                     /db_xref="ENSEMBL:ENSMUSE00000300251"
                     /rank="2"
                     /note="ENSMUSE00000300251"
     misc_feature    5434..10758
                     /note="5 arm"
     misc_feature    10786..10810
                     /note="B1 Gateway"
     misc_feature    10823..10870
                     /note="Frt"
     intron          10871..11745
                     /note="mouse En2 intron"
     misc_feature    11743..11745
                     /note="Splice Acceptor"
     exon            11746..11918
                     /note="mouse En2 exon"
     misc_feature    11930..12523
                     /note="ECMV IRES"
     gene            12524..15584
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    15623..15866
                     /note="SV40 polyadenylation site"
     misc_feature    15870..15903
                     /note="loxP"
     promoter        15930..16449
                     /note="human beta actin promoter"
     gene            16475..17269
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    17469..17710
                     /note="SV40 polyadenylation site"
     misc_feature    17727..17774
                     /note="Frt"
     misc_feature    17781..17814
                     /note="loxP"
     misc_feature    11760..11837
                     /note="FCHK std_gpr"
     misc_feature    17830..17850
                     /note="B2 Gateway"
     misc_feature    10759..17880
                     /note="Synthetic Cassette"
     exon            18331..19752
                     /db_xref="ENSEMBL:ENSMUSE00000300242"
                     /type="targeted"
                     /rank="3"
                     /note="target exon 1 ENSMUSE00000300242 5' fragment"
     misc_feature    17881..19752
                     /note="Critical Region"
     misc_feature    19776..19809
                     /note="Downstream LoxP"
     misc_feature    19753..19832
                     /note="synthetic loxP region"
     exon            19833..20523
                     /db_xref="ENSEMBL:ENSMUSE00000300242"
                     /rank="3"
                     /note="ENSMUSE00000300242 3' fragment"
     misc_feature    19833..24077
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca actttgtata gaaaagttga acgagaaacg
       61 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
      121 tactgtaaaa cacaacatat ccagtcacta tggcggccgc aatcgggcag cgttgggtcc
      181 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      241 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
      301 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
      361 agtctggaaa cgggccgcct agcgatagct gtttatcgaa ttctaccggg taggggaggc
      421 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      481 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      541 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      601 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      661 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      721 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      781 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      841 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      901 cctttcgacc tgcaggtcct cgccatggat cctgatgatg ttgttgattc ttctaaatct
      961 tttgtgatgg aaaacttttc ttcgtaccac gggactaaac ctggttatgt agattccatt
     1021 caaaaaggta tacaaaagcc aaaatctggt acacaaggaa attatgacga tgattggaaa
     1081 gggttttata gtaccgacaa taaatacgac gctgcgggat actctgtaga taatgaaaac
     1141 ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt atccaggact gacgaaggtt
     1201 ctcgcactaa aagtggataa tgccgaaact attaagaaag agttaggttt aagtctcact
     1261 gaaccgttga tggagcaagt cggaacggaa gagtttatca aaaggttcgg tgatggtgct
     1321 tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt ctagcgttga atatattaat
     1381 aactgggaac aggcgaaagc gttaagcgta gaacttgaga ttaattttga aacccgtgga
     1441 aaacgtggcc aagatgcgat gtatgagtat atggctcaag cctgtgcagg aaatcgtgtc
     1501 aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     1561 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     1621 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     1681 gtggtggaat gcagatccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     1921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag
     1981 atccactagt tctagcctcg aggctagagc ggccgagttt atcctcgagc acgtgttgac
     2041 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     2101 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     2161 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     2221 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     2281 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     2341 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     2401 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     2461 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
     2521 ggactgagaa ttccccggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     2581 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     2641 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     2701 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     2761 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     2821 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     2881 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2941 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3001 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3061 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3121 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     3181 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3241 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3301 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3841 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4321 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4501 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4801 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5221 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga aaattgtcga ccatagtgac
     5281 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     5341 atattgatat ttatatcatt ttacgtttct cgttcaactt tattatacaa agttgcggac
     5401 gcgatcgccg gcggataaca atttcacaca ggaacccaaa acgcataagt gttgtagagt
     5461 gggaacacat tggcaaattt ctccaagctt tgtgctgtgt tttcccaaca cacatggctt
     5521 gtcacttcag agtcaaaaca aatcccatga gcaagggtca gaagagcaag agtgttgatg
     5581 gttgactcaa ataaatggct gtcactctgg gttcctaaca ggttgccgtc taagaggtag
     5641 ggtggcctcg ctgatgaaag gaagcttccg cctcactcag gaaagcacct gcagctctgg
     5701 gccacatatg tcccacaata gctatggatg tacccaccac acttatggac aatcacaatg
     5761 tcaaaaggcc agtcttaggt cagatccatg ctccagatga ccactgagga gtcggaaggg
     5821 caatgctgta taccggccat gctcagactc tgggatcatg ttcccgagga ctgtcaggca
     5881 agaggtgacc ctagacccat gaagttctgg ggctgatcta tgctgtggaa gacggcagga
     5941 aggccaagag agagaagttt aggtggtggt tgattggcgc gggggggcgt gggggggggg
     6001 cggggggcgg ggggtggggg gcgggggggg gcgttggtgg cactctggat cctgaccttt
     6061 gaaacatgac tgggttgtgt gctgggagca cctgtgaaga aggttcatga aggcgttaca
     6121 ttactgcacc tgctcctcat atatgatcca atactgtctg tatatggtgt gggcctgcca
     6181 ctgaacttct caggcctctc cctttagcct tgagtcactg atggagtgtg aactctgcag
     6241 cccaggccct tcctgccatg gtacagcagc cagtgtaggt tcctcagata caacaccatt
     6301 ataaacaact gtagacttgt gcacaaaaca tattgccaaa cccacataga catcaaactc
     6361 aaatggggcc aagcagacca ggaagaacat gagcaggctg gtccaaggac aaggaccctg
     6421 gcccagactc agccaccagc ctgccttcac ctgcaaatgt tcatgctgta ttcctcaggc
     6481 tctgtcctct atcaagaagc caagcccaga ggcagcctag atcggagact acaaaggaag
     6541 ctctcaccaa agagggactt gcaccgtact gacagaatct ttaaggtctc tctcatccct
     6601 tatccatact aaaacctatg gagatcagaa ggcagacgtg ccctctgcct gggtcctgtt
     6661 gtgcctctca gcagagtggt agactagacg taggatgtga ctacctggaa tgttgaaggg
     6721 aaggctgggc tcagagtaag atgcgccccc aagacttcag ctgccagtcc tgcatggcat
     6781 cagagcaccc caggggactc ccaggaaccc agaagacagc aaaggccctc tcagtggtcc
     6841 aacaccatgg aacttgacca tgggcttgca agagtgggga aattctaagg gagcggctat
     6901 ttttaagtgg cattccttgg ctatttcaag gcttgggttt ctgttttatt tatttaattc
     6961 aaaaggaagc tgtgttttct cagcatttgt ttctttctcg atggtgtcgc aggcattgtt
     7021 ttggtgctga cgtcatcctg acttggtaag ctggtgaaaa gatactgaag ggccaagggg
     7081 ctcttcccat gctgctcaaa tgagaaaaag ccaactccgt agtcagggct tctacagctc
     7141 aaaggggccc taacagtatt tttccactaa aaattgtatt tttaatggaa ctctatttaa
     7201 agaagtgtac atttcaaaaa atgcatatct caaagattct aagccagttt cctatagaaa
     7261 gccaccaaac atgtttctag caggaagcta cacacgtggg cacagggcag ctgatcgttg
     7321 ttttctagag accctgtagc tgtctacatg cagctaactg cttcatctgt tcataaacat
     7381 attttatgtg tatgactgtt ttaccggcat gggtgtcacc tgtgtgcctg gttcccagag
     7441 tctgagagtg tgggttggat ctcctaggtc tggagttaca gatgatggtg agccacccta
     7501 ggggtgctgg gaattggacc tctgtcctct ttaagagcaa caagtgttaa ctgccgagcc
     7561 acctctccaa cccagtatct gcttcttcct gctgcacaga gtcccccttt agaggagcag
     7621 catgtggtgg tttctgcagt cctcagccag cagaggtatg caatattcat gctcagaaca
     7681 caaatgccct ctcaatgtat tcaacatcac agattgccga caatcaaaaa gacatcaagc
     7741 taaaaacaca gggggctgca ctgagtggga agaggtggga ccctgggggt ctcttgccag
     7801 ccatcctacc tgttttggtg agccctaggc tgatcagtga ccctgccata aacaaacaaa
     7861 caaacaaaca aacaaacaag gtggacagaa cctgaggact agcaaacagg ttctctagcc
     7921 tctacatcca catacattta tacctacata cataaacatg cacacacact cagatacaaa
     7981 gaagagtgca caaatgagac caacgaactc taaatcatta cgaatacata atcacttgac
     8041 tggattgctc aggtcggggc tgcaggaggt ccgattatgt ggcgaagccc tgttccccat
     8101 gtttgtctcc acccccaggg ccacaattct atgagaaccc cagtgttgtg gatacgtact
     8161 aagtaagtta caaggccact tccccccccc cccaccttgg ttgtacacgg tctagaacta
     8221 gagacagagc tgacccctgg gtagagggac attagcccta gctaacatta gagtagaaaa
     8281 cacacctgga ttcctaattt ctggtcaaaa tgccggctac ttgtagactg tatttcccac
     8341 ttcgagcatc cctaggaaaa ctatagctta catttgctgt gtgactgcag tcctaccatg
     8401 ctgattgagg aaactcagtg ttcagtttta aagccagcac aagctaaaac aggcaagggt
     8461 atcattgctt caacaaatga ggaaggattc ttacctaaaa ggtaaacaat attataatcc
     8521 tttcacactt taaataaatg tgtcagtggg tgaaaatcag cagtcacact ggaaacttcc
     8581 ataaaataca catccagcag ggtcgttttc actgtctaca tgtgggagga aaaaaactgc
     8641 cagcaaaaaa atggttaatg ctggccttaa agggaggctg gccagagccc agtgagtagg
     8701 cttgggaagt ctataaagga ggcgctgtgc caagggggcc agacgctgct ggacggccac
     8761 aggcatccat ccccaggcat cgggcgggtg cttctgttcc tctcctgctg ggtccctgct
     8821 gggcaccgtc cccaaagtgc tgcaagtcca gggtccatcc ctggagcaag ctccaggagc
     8881 acctccagca gatggcctgg taacgccacg gcacaggtaa gattcacact caggttccgg
     8941 gactgcgtgt ggctcggaac tccttgctgg ggtggggtgg cactggtggg gcagtcatgc
     9001 accctcggcc tccaggattg cacgataggt gtgagcaagg tctatccagg cgcaggcaac
     9061 aacagcatgc ctacctaaag ctcggccaca cttgggtcct ggctacgccc tgtaacacca
     9121 cgatgctgcc actctgtctg ccaagggagt cctggaagct tacaactgtt tcaaggacag
     9181 gggcctcgta agcctggcaa aaacctagtc ttgtttcccc agatcaggac acccaacaga
     9241 aaatcagaag gacactaagt ctgatcgtta gattaacaga gcagcgatct ggaccaagga
     9301 cagaagccag ggtgtcattt ctgccatgca cccaccaaaa cagctgatca gatctaggga
     9361 gaaagccatc caaggactct gctcccctta agctgctgga attgtggcca agcctcaaca
     9421 ctcacacact ctgggtgagt acttttaaat tttcaggttt tgtttgtttg tttgtttcag
     9481 ccctgactgg catcaaactc acttcataga ctgggctggc ctcaaactta gagctccact
     9541 tgcctctcat ccttttgtgc tgggattaaa gtatgcacca ggctcaggta tcagaagaaa
     9601 tgaagggaaa ccctctgcat tgtagctaga ctcagaagac tggcgcttct gtggctcaga
     9661 tgggcacgtc catgaaagat gggttggcca cccacaccca cacatccaag gcaggatgtg
     9721 gccacagccc ctcctttctt cttcccatca ttttaatgaa gcagtatttg gggagagaaa
     9781 ggccaattta aacctctggt ttgctcttgc tccacctcaa aaccaaatgc aatcaccaca
     9841 agcaaagcaa cagagatgtg ttttcaggag cacatctgag gagcactttg ctgtctcgct
     9901 ctgactgtga aggaagacac agggacagta gctgccatgt ccctgcccgg ggcaatatgg
     9961 acacttagta gcattctcag cttccactca ccagatgcca gcacccatct cctacacagt
    10021 gacaccctag ggtctcgaga cactgctagt gccgctgaga acctggatta ccccagagag
    10081 ccccgaggca gagtcataca ggctaggaac catccccagg gaccaatgaa gctcagcttc
    10141 caggctgcag tgcctaaaat gttgtatttc acaggaactt atgtaaagga caagctgctt
    10201 cccttcttct ctcctgtctt ccttattcct tgagatcggg gtctcaggct gctgcctgag
    10261 ctagtctaaa acgcctgtgc tccagggatc ctccggagca caacctccag agtagccggg
    10321 aggacgagga gtcatcccct actgtttagt gaatttggaa cagcctggac tataagagac
    10381 cctgcctcaa aaccagctgt gtgagctggg tgttggtggc acacgccttt taatcccagc
    10441 actcgggagg cagaggcagg cggatttctg agttcgaggc cagcctggtc tacagagtga
    10501 gttccaggac agcaagagct acatagcaaa aactcatctc aggaaaaaat gatacagctc
    10561 tctgtgtgtg tgcgcgtgtg tgcacgcgcg tgcacacaca tgcacacaca cgcacacaag
    10621 agaaagatac tgaaggtgga acccacagct ctcttgtgtg ccagataaat gttttaccac
    10681 tgagctacat cccagtcctc caataacaat gcttgttctt tacttgtttt ccctttaaac
    10741 gatggcaggt gtctcctcaa ggcgcataac gataccacga tatcaacaag tttgtacaaa
    10801 aaagcaggct ggcgccggaa ccgaagttcc tattccgaag ttcctattct ctagaaagta
    10861 taggaacttc gaaccctttc ccacaccacc ctccacactt gccccaaaca ctgccaacta
    10921 tgtaggagga aggggttggg actaacagaa gaacccgttg tggggaagct gttgggaggg
    10981 tcactttatg ttcttgccca aggtcagttg ggtggcctgc ttctgatgag gtggtcccaa
    11041 ggtctggggt agaaggtgag agggacaggc caccaaggtc agcccccccc ccctatccca
    11101 taggagccag gtccctctcc tggacaggaa gactgaaggg gagatgccag agactcagtg
    11161 aagcctgggg taccctattg gagtccttca aggaaacaaa cttggcctca ccaggcctca
    11221 gccttggctc ctcctgggaa ctctactgcc cttgggatcc ccttgtagtt gtgggttaca
    11281 taggaagggg gacgggattc cccttgactg gctagcctac tcttttcttc agtcttctcc
    11341 atctcctctc acctgtctct cgaccctttc cctaggatag acttggaaaa agataagggg
    11401 agaaaacaaa tgcaaacgag gccagaaaga ttttggctgg gcattccttc cgctagcttt
    11461 tattgggatc ccctagtttg tgataggcct tttagctaca tctgccaatc catctcattt
    11521 tcacacacac acacaccact ttccttctgg tcagtgggca catgtccagc ctcaagttta
    11581 tatcaccacc cccaatgccc aacacttgta tggccttggg cgggtcatcc ccccccccac
    11641 ccccagtatc tgcaacctca agctagcttg ggtgcgttgg ttgtggataa gtagctagac
    11701 tccagcaacc agtaacctct gccctttctc ctccatgaca accaggtccc aggtcccgaa
    11761 aaccaaagaa gaagaaccct aacaaagagg acaagcggcc tcgcacagcc ttcactgctg
    11821 agcagctcca gaggctcaag gctgagtttc agaccaacag gtacctgaca gagcagcggc
    11881 gccagagtct ggcacaggag ctcggtaccc ggaagatctg gactctagag aattccgccc
    11941 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
    12001 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
    12061 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
    12121 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
    12181 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
    12241 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
    12301 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
    12361 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
    12421 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac
    12481 gtggttttcc tttgaaaaac acgatgataa gcttgccaca accatggaag atcccgtcgt
    12541 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
    12601 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
    12661 gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc
    12721 ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg
    12781 gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa
    12841 tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga
    12901 tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt
    12961 tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga
    13021 atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg
    13081 ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg
    13141 tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg
    13201 ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt
    13261 gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg
    13321 caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac
    13381 actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg
    13441 tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt
    13501 cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct
    13561 gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca
    13621 gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc
    13681 gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt
    13741 ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc
    13801 gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca
    13861 cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc
    13921 gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg
    13981 agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca
    14041 gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac
    14101 gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc
    14161 taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg
    14221 ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg
    14281 tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga
    14341 ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg
    14401 tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga
    14461 gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct
    14521 ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag
    14581 cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga
    14641 agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct
    14701 ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga
    14761 gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat
    14821 tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga
    14881 taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg
    14941 gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact
    15001 tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt
    15061 tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt
    15121 tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca
    15181 ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct
    15241 tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt
    15301 cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca
    15361 gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac
    15421 cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca
    15481 tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat tccagctgag
    15541 cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac cgggcagggg
    15601 ggatctaagc tctagataag taatgatcat aatcagccat atcacatctg tagaggtttt
    15661 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
    15721 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    15781 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    15841 caatgtatct tatcatgtct ggatccggaa taacttcgta tagcatacat tatacgaagt
    15901 tatgtttaaa cggcgcgccc cggaattcgc cttctgcagg agcgtacaga acccagggcc
    15961 ctggcacccg tgcagaccct ggcccacccc acctgggcgc tcagtgccca agagatgtcc
    16021 acacctagga tgtcccgcgg tgggtggggg gcccgagaga cgggcaggcc gggggcaggc
    16081 ctggccatgc ggggccgaac cgggcactgc ccagcgtggg cgcgggggcc acggcgcgcg
    16141 cccccagccc ccgggcccag caccccaagg cggccaacgc caaaactctc cctcctcctc
    16201 ttcctcaatc tcgctctcgc tctttttttt tttcgcaaaa ggaggggaga gggggtaaaa
    16261 aaatgctgca ctgtgcggcg aagccggtga gtgagcggcg cggggccaat cagcgtgcgc
    16321 cgttccgaaa gttgcctttt atggctcgag cggccgcggc ggcgccctat aaaacccagc
    16381 ggcgcgacgc gccaccaccg ccgagaccgc gtccgccccg cgagcacaga gcctcgcctt
    16441 tgccgatcct ctagagtcga gatccgccgc caccatgatt gaacaagatg gattgcacgc
    16501 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
    16561 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
    16621 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
    16681 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
    16741 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
    16801 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
    16861 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
    16921 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
    16981 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
    17041 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
    17101 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
    17161 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
    17221 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
    17281 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
    17341 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
    17401 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccccggatct aagctctaga
    17461 taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac
    17521 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    17581 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    17641 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    17701 gtctggatcc gggggtaccg cgtcgagaag ttcctattcc gaagttccta ttctctagaa
    17761 agtataggaa cttcgtcgag ataacttcgt atagcataca ttatacgaag ttatgtcgag
    17821 atatctagac ccagctttct tgtacaaagt ggttgatatc tctatagtcg cagtaggcgg
    17881 gaacaaaatt tctcttccca ctttactgca agagaaaagg cagctcccag cccttgggtt
    17941 cttggactac ctttgagaaa ggctgggttt ttcccttccc atgtcacgtc tctttccaac
    18001 agctgctgaa gttaatgaga cttcctgtct cgacaaacag tagttttcct caaattccca
    18061 caccacacac tcacggtatt tcacgggcca gtgatctgac tggtcccact acatctccca
    18121 tgactcacag actgtcacca actgtgggcg ctcttaggga acataaagga acactccacg
    18181 tctatccacg tctatctctt ctgctccagc tcaggggggt ggcgaaagcc agagtccagg
    18241 tggaccccgt tgtcactcat gcaccacacc tggctccttc aggatgctca gggttctaga
    18301 tgctaagtct tgccccgtct gcttctacag gtgcccagaa ggtgagcagg cagcaggtgt
    18361 gcctgcccag caccagccca gacatcagac accctgtcca cccttctggt tttctgagac
    18421 taacaggctc ccaggacgat tcttcctgcc tcacaaatgc ctggtttatc tttctttgtg
    18481 aagatggagc tgtcacataa aacagctttc tgtgaccctt tcagcaaatc ctgaaaactg
    18541 ccgagggaag ccatggatgc gactactcca gcccaaactg ttggggtgga gatctaccta
    18601 ggtcccgtgt ggccagcccc ttccaacagc acccctctgg ccctcaactt gtccctggca
    18661 ctgcgggaag atgccccggg gaacctcact ggggacctct ctgagcatca gcagtacgtg
    18721 attgccctct tcctctcctg cctctacacc atcttcctct ttcctattgg ctttgtgggc
    18781 aacatcctca tcctggtggt gaacatcagc ttccgggaga agatgaccat cccagacctg
    18841 tacttcatca acctggcggc ggccgacctc atcctggtgg ctgactccct gattgaggtg
    18901 ttcaacctgg acgagcagta ctacgacatc gcagtgctct gcaccttcat gtccctcttc
    18961 ctgcagatca acatgtacag cagcgtcttc ttcctcacct ggatgagctt cgacaggtac
    19021 ctagcgctgg ccaaggccat gcgctgtggc ctcttccgca ccaagcacca cgcacggctc
    19081 agctgtggcc tcatctggat ggcctcagtg tccgccacgc tggtgccctt cacagcggtg
    19141 cacctgcggc acacggagga ggcctgcttc tgctttgctg atgtcaggga ggtgcagtgg
    19201 ctggaggtca cactgggctt catcatgccc ttcgccatca ttggcctctg ctactccctc
    19261 atcgtgcgag ccctcatccg ggcccacagg caccgcggcc tgcgcccacg caggcagaaa
    19321 gccctgagga tgatcttcgc agtggtcctt gttttcttca tctgctggct gccggagaac
    19381 gtcttcatca gtgtccacct actgcagtgg acgcagccag gggacactcc ctgcaagcag
    19441 tctttccgtc acgcctaccc cttgacaggc cacatagtca accttgcagc cttctccaac
    19501 agctgcctga atcccctcat ctacagcttc ctgggagaga ccttcaggga caagctcagg
    19561 ctctatgtgg agcagaagac gagcctgccg gctctgaacc gcttctgcca tgccacgctc
    19621 aaggccgtca ttccagacag cacagagcag tcagaggtca ggttcagcag tgctgtgtga
    19681 gaggaaaagg tcaggggcgc aggctggtgc tcaggacttg cacacaccta gcacaggtgg
    19741 tgagtgggct aagagatggc gcaacgcaat taatgataac ttcgtatagc atacattata
    19801 cgaagttatg gtctgagctc gccatcagtt caatactctc aaaccccagt ggcttgggga
    19861 agacgtcaca ttgcggggtc atctctggag ctgctggcat ccttcctgac tgtccagctc
    19921 atggatgctg ccatccagat tcaaggtccc aaggcagcgg gccacgtgac attgacctct
    19981 gacctcaaag ggcaccaggc gggcctgctg cttggctttc tttccatagc ctacgttccc
    20041 agaacacaag tctgctgttg atacgaggac aggccatgct atgggagcac catgttacat
    20101 gcctgctacg tggaggagtc tagagacaga ctttatgtac cagacccaaa ctggctacct
    20161 tccctttgct tgtgatgtgt aactgaccat gtatacaccg tccagtgcag ccagagcctt
    20221 cttcctgtct tccagaaggc tgtgaggtca ccccagatgc cactcctaac tcctgagtga
    20281 acagcgtgtc tgactgagaa aggcccttta acaaaacgcc ttcctgctct gggatgctcc
    20341 tctcacaaag tttgtttaca aaggtgtttg cccttccgtg aaggtggaag gagactgggt
    20401 gctgctgtgc aggctggtgg gatgccgcca taagatgtgt ggtagaagga cttaccacca
    20461 cagaaaatca tactgggaac agcgagctgt aaatggatct cattaaaacg tgaaaagaac
    20521 caaggatggg tggtcattca gcgtctgcga attctctctg gggtggcagg gcgaccgggg
    20581 gggggggggg gggggttgtt tagaaagcca tgctagtaca gtcagctgct cccttgcaaa
    20641 ggaactctat gcccgtaccc tcacatacct gaggaccatt ctgcgtactc tcctatgtac
    20701 caacagccaa tctgtctaga tggctacgcc tggcacttct gggatggaga gggccacatt
    20761 cggtgtttca cttgggcact aaacatgtct tccttgttac ttatgttgta agccacgctt
    20821 agtctcattt tggggtctgc ataggacagc tatctgtgcc tagaagattc tgtctgggcg
    20881 acatgcccag tgctaggcgt cagagggcct ggggccagga gggagggcct ctggccctgt
    20941 atcacaggac aaactgcttt acacgccgcc tctacttcct gaacacctgc ctgggcagtt
    21001 gtccacgtca agaggcccat ggctctggct gtggacccta gggtaacata caaggtaaca
    21061 agtaggcgat aaaatgaccc acattgtctc taaagaatat gctattttcc attcaaacct
    21121 atgaccctgc ctttttattc tgatgatgtt atatttatag ataagattaa gttaaaacaa
    21181 tcacgtggta tttaagttat taaggtaaat attagagcta tttaccttat ttaagtatct
    21241 ttccagatac tcaagatact caagaacagt atctggaaag agagcagatg gtttagtgga
    21301 cttggcagag ccgaggagtc agacaaacta cacaggacac aggtttcccc tccctcggtg
    21361 ttttgtcagg cacacctggt gcggggggtg gcatggccag cagcctggag tgcattccgg
    21421 gccggcttta gtgaccctga gtattagtct tcaagaacaa gagcatttgt ccccaactgt
    21481 gacctgggtc ccaagcaggg ctggctttct acctgggcag ggcacgggat gcctcctcaa
    21541 ggatggcgct cggaagccaa cagtgaagaa ccaccatggg aggtgggtgg tgaaaagcct
    21601 ggtgtgtgtc tctgtcttgc aaggcccacc aactcagaag cccttggctc caccccagcc
    21661 accccaacac acgtgtatac agctcatgca aaccttgcag aatcaggagg taccttgacc
    21721 ctgtcacttc tgtcagcaac ctctgagccc caggggatgt ggccagctct cacagtgccc
    21781 ctaaagacag gcacaaagca ggcattacag tggaggctgg gaagtgtggc tgcaaacccc
    21841 aatgggggaa catcattgta aaccgctggt tcaaactgcc ctttgatctc agtggttgac
    21901 aagaaaagac cctccattcc aagtcaggaa aaggcaggac tgagcctcaa ctcctcaaat
    21961 gtgcctacgt gagaggcacc tgctggctgt tcttggacca gtgtgatacg caggtgccta
    22021 aaatatgcca aggcttccaa ctttggctag ggaggcagcc aggcatcagg aagaggacag
    22081 caggcccctg aggacacgcc cccatccata ttatatagag aaaaactaga tgcttggagt
    22141 ctgtgaacgt caaaaccacg gacaataaag gatggggcca ggaacagaac ctcagcctcc
    22201 aacccactgg gcagctctgc agcctggaca gggaagacga tggggagcca gggcacacag
    22261 aaggcagcca gggttaccct tccctcttta tggctacaac cccagggctt atagttacaa
    22321 ctgcagctgc ccaaagtccc agaacacttc tcgagacagc acgccttcct tgtggcaagc
    22381 tacccttggg tcagtggcac cctccacagt cctctggaca gcagcacagt ggtccatggg
    22441 gactactgta gtcctaagat catgcaggac aggcccactc tgctgcatac aactgtagcc
    22501 ccttgacgca atgtcacctt ctatctaagg ctcctgacca gagtcttcta caagctaagc
    22561 tatgccaggc tggagccagg cagggttgga acacagctcc tgcctcctct atgcctcaaa
    22621 gcacacttgc tatctgcaag cataaggaga gctgctctgg gggtggggag ggtggatgac
    22681 tgatgtatat ccccaagtcc cagggcaagg aggccagaag ggtaaggcaa aaaagaaggc
    22741 tggacttcgg ggacagagca aggcagggct ggctctacgg gccctgtgca gcctgtgtga
    22801 cacccccccg cccccccccc cccgagcgcg tccaggagcc gagctgaggc taacacaatt
    22861 attcttggca ggaacaccac aagataaaat caaggcgttt taattgcact gctgtttcct
    22921 ggtaacctgg ctcactcccg cgtacaaatg tagttcagac cctaagaagg ggagaggcag
    22981 ctaccgtggg catggaaaac ctgcttagaa ctggaaacca ttccatcacc tactcagagc
    23041 ttaataacga gccctccctt gcactccagc ccatccgccc tggtgagcaa gacctaaggt
    23101 accatcaatt ctcttcttgt tttggtgggc agagaccagc cacctggctg cctaggagag
    23161 gcaaagatac aggatgatca gcaagcttga tagcacactc atcttttggg gttcttttcc
    23221 ccccaaaatc tgatgcagta tctcagtata gaggctcttc tgctgctgtc ctgaacacgt
    23281 acaccaggga gcagcctccc cttcacgcct ccataagcat gtgtaaaatt catccccaac
    23341 tgtctctgat tcattagtgc tttttaaaat gtgtgtgtgt gtttgtgtgt gtgcatgtgc
    23401 acgcatgcgt gtatggtgta tgcaggggct ggggaaacgg gatgggagtt gtacttgtct
    23461 gtgtatgcat gtgtggaggt caaaggttaa tgtaggatgt cttcccctat tgcacctgat
    23521 gataaaaatc ctatttcttg aggcaggctc tgtcactgag cagtgatccc agggatccgc
    23581 ctgtacctgt gtgtttgtgc acatgtgcgt gtgcatacac acacacacac acaaatggta
    23641 agcattaaac tctcactaac acacatgcct tcagccctgt tagaagggtg ggaacagaca
    23701 caaacccgag gaggctatct tctgcagtac tagggatgaa cgctgaaacc gctcggctgt
    23761 gttgtgtcca accgagcaca cagggctcca cttgctcaga gtctaggacc aaaggagggc
    23821 agccccagca gcctggcccc tcagagacct ggcaggttca gggtgggagt ggctgggaca
    23881 tgtggttctc agtgagaaca gccccgagag gaaagggtca catccaggac agaggaggtg
    23941 gcctaagagc ctcccatggg ccaaactggg atgacctgag catcaggata attaagagga
    24001 atcaacagtt acaaacagtc atagactatt aaagagcaac caggactcca ttccagcaat
    24061 aactggataa atataac
//