LOCUS       KO-first_condition_ready_35966_MGI:1923011        22399 bp    dna     circular UNK 
DEFINITION  Mus musculus targeted KO-first, conditional ready, lacZ-tagged
            mutant vector Apol7a targeting project(s): 35966
ACCESSION   unknown
SOURCE      Mus musculus
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;
            Sciurognathi; Muroidea; Muridae; Murinae; Mus.
COMMENT     cassette: L1L2_Pgk_P
COMMENT     design_id: 115386
COMMENT     backbone: L3L4_pD223_DTA_spec
FEATURES             Location/Qualifiers
     misc_feature    29..49
                     /note="B4 Gateway"
     misc_feature    complement(125..378)
                     /note="BGH pA"
     CDS             complement(503..1159)
                     /note="DTA"
     promoter        complement(1173..1685)
                     /note="PGK promoter"
     promoter        1686..1836
                     /note="spectinomycin promoter"
     CDS             1837..2847
                     /note="SpecR"
     misc_feature    2995..3587
                     /note="pUC ori"
     misc_feature    3858..3885
                     /note="rrnB T2"
     misc_feature    4017..4060
                     /note="rrnB T1"
     misc_feature    4156..4176
                     /note="B3 Gateway"
     misc_feature    4182..4189
                     /note="AsiSI"
     misc_feature    1..4214
                     /note="Synthetic Backbone"
     exon            7332..7419
                     /db_xref="ENSEMBL:ENSMUSE00001291831"
                     /rank="3"
                     /note="ENSMUSE00001291831"
     exon            7143..7242
                     /db_xref="ENSEMBL:ENSMUSE00000681579"
                     /rank="2"
                     /note="ENSMUSE00000681579"
     exon            4539..4711
                     /db_xref="ENSEMBL:ENSMUSE00000949808"
                     /rank="1"
                     /note="ENSMUSE00000949808"
     misc_feature    4215..9461
                     /note="5 arm"
     misc_feature    9489..9513
                     /note="B1 Gateway"
     misc_feature    9526..9573
                     /note="Frt"
     intron          9574..10448
                     /note="mouse En2 intron"
     misc_feature    10446..10448
                     /note="Splice Acceptor"
     exon            10449..10450
                     /note="mouse En2 exon"
     misc_feature    10662..11223
                     /note="ECMV IRES"
     gene            11234..14280
                     /gene="lacZ"
                     /note="lacZ"
     misc_feature    14315..14570
                     /note="SV40 polyadenylation site"
     misc_feature    14574..14607
                     /note="loxP"
     promoter        14639..15146
                     /note="mouse phosphoglycerate kinase 1 promoter"
     gene            15233..16024
                     /gene="NeoR"
                     /note="NeoR"
     misc_feature    16222..16465
                     /note="SV40 polyadenylation site"
     misc_feature    16482..16529
                     /note="Frt"
     misc_feature    16536..16569
                     /note="loxP"
     misc_feature    10463..10540
                     /note="FCHK std_gpr"
     misc_feature    16585..16605
                     /note="B2 Gateway"
     misc_feature    9462..16635
                     /note="Synthetic Cassette"
     exon            17951..18055
                     /db_xref="ENSEMBL:ENSMUSE00001086370"
                     /type="targeted"
                     /rank="5"
                     /note="target exon 1 ENSMUSE00001086370"
     exon            17277..17403
                     /db_xref="ENSEMBL:ENSMUSE00001034102"
                     /type="targeted"
                     /rank="4"
                     /note="target exon 2 ENSMUSE00001034102"
     misc_feature    16636..18245
                     /note="Critical Region"
     misc_feature    18269..18302
                     /note="Downstream LoxP"
     misc_feature    18246..18325
                     /note="synthetic loxP region"
     exon            20777..22399
                     /db_xref="ENSEMBL:ENSMUSE00000951562"
                     /rank="6"
                     /note="ENSMUSE00000951562 5' fragment"
     misc_feature    18326..22399
                     /note="3 arm"
ORIGIN      
        1 ccactggccg tcgttttaca ttaattaaca acttttctat acaaagttga cagatatctg
       61 tttaaactcg gccgctctag cctcgaggct agaactagtg gatctcgagc cccagctggt
      121 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
      181 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
      241 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
      301 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
      361 agtcgaggct gatcagcgag ctctaggatc tgcattccac cactgctccc attcatcagt
      421 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
      481 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
      541 tgtcacacca cagaagtaag gttccttcac aaagagatcg cctgacacga tttcctgcac
      601 aggcttgagc catatactca tacatcgcat cttggccacg ttttccacgg gtttcaaaat
      661 taatctcaag ttctacgctt aacgctttcg cctgttccca gttattaata tattcaacgc
      721 tagaactccc ctcagcgaag ggaaggctga gcactacacg cgaagcacca tcaccgaacc
      781 ttttgataaa ctcttccgtt ccgacttgct ccatcaacgg ttcagtgaga cttaaaccta
      841 actctttctt aatagtttcg gcattatcca cttttagtgc gagaaccttc gtcagtcctg
      901 gatacgtcac tttgaccacg cctccagctt ttccagagag cgggttttca ttatctacag
      961 agtatcccgc agcgtcgtat ttattgtcgg tactataaaa ccctttccaa tcatcgtcat
     1021 aatttccttg tgtaccagat tttggctttt gtataccttt ttgaatggaa tctacataac
     1081 caggtttagt cccgtggtac gaagaaaagt tttccatcac aaaagattta gaagaatcaa
     1141 caacatcatc aggatccatg gcgaggacct gcaggtcgaa aggcccggag atgaggaaga
     1201 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     1261 ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct
     1321 tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag
     1381 gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc
     1441 tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac
     1501 taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct
     1561 accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag
     1621 cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta
     1681 gaattcgata aacagctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     1741 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     1801 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1981 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2041 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2101 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2161 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2221 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2281 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2341 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2401 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     2461 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     2521 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     2581 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     2641 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     2701 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     2761 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     2821 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     2881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtcttaaat cgtgccaact ttattataca aagttgcgga cgcgatcgcc ggcggataac
     4201 aatttcacac aggaggtgct tctcagccat tcggtattcc ttgaaccagt ttaaacaaca
     4261 tgctaacaca gtgaacctat acgcaggcac gcacacagtt cccgatcaca cttagctcca
     4321 tacagctgtg tatttcaaag ttcattacac acatttccaa aagtttttgt gtcctaacag
     4381 tttttgcctc taggtggcgc agcacccatg tgtgctgtgg actctgtttc ggtgctatga
     4441 tggctgttag gacagaccaa ctgagaaggt aaggccagcc tctaatctgg gccacacctg
     4501 ctggaagctt acataaagac tggaagaagg aagcttttgc tcttgccggc ttgccgcacc
     4561 tcactagcaa gtccgtttct acaatggcac taggattcca gagtgtacta aaaaccagct
     4621 gagacatcca gccctgtgga ctgagcagct acgggagtct ccaacctgcc atgcgtagac
     4681 agcctttgtt ggactagcta aaccagagtc tgtgagtcat tctaattcat gaatctatat
     4741 taactctata tacagagatt ctatatacag agagaggcgc cttctgtaag ctctgttgct
     4801 ctagagaacc ctgatgagga cagatggtca gttcccccta ccccactctg gccttacaag
     4861 cgagctagtc tgagcttcgc ctcagacccc tgcacaaggt ttcctctgct cactctgctg
     4921 cctgcttgag atatggatgt gtggccccac ctgggcatcc gacaggcctg gcagggtgtt
     4981 gtgactgtcc tataaaactc cacatcctgc aacctgacag ctacagcaga gatgtgcccc
     5041 aagagcagga tcaatagaca tcttcccaac atgtaaattc tttggccact gggtgagctt
     5101 taaagtgcat ctttgtattt atatgatttt ctttttttta ctgatgagat gactccaccc
     5161 ctgtccccta aagtcccaag tccctgttag cagcattgaa caggagtctg ggcctcagga
     5221 gctgactttg gtttcccttt ggggagggga cttcagaaca catgccatgt ctcagactct
     5281 gacctgttct tccgtttctt aaaagattct cttcctatca tcttttgact tcatgtgagt
     5341 atgggaaagc ttgagcgagg gggtgggggt cgggggacac ggctgctatc ctggcacttg
     5401 ggtggtgcag gcctaagatt aggagttcaa gattctctca acctacacag tgcgtgtgag
     5461 gccagacgag gctacatgga cacttggaga gctttggcaa gaaaagggag ttatgggtta
     5521 gaagggtgga gagtggttct gagactgagt caccactgtc ttcagctctc accatagcaa
     5581 cacgtacatt cattctccgt ttagtcaggt cccaggagga ggggctggcg ccttttccct
     5641 atactgctaa ccgcagtttc tttccttctt ccttagctcc caaggcaatc tcagtgcagt
     5701 ttctgtttct gtttttatga ggtccacccc tctgcaaaat cacctgatca ctggtgagaa
     5761 caaggagtga acactctcac acacggcctg tgtttagatg tgaacgtcct ttaacccagt
     5821 tatctactta tacaaatagc ataaggttaa tctcaaatgc aggcactgac aggaatgagg
     5881 acattctctg cattagtatt tacatatgat aaggtccagg agtcatggag acaagcagga
     5941 gtccagggca gcccacggcc cggcagggac caggcaggac tccagggtct gatgggacaa
     6001 ggatatcaca gaagcaagga gggtgtggtc cctgggaagc cacaggtgac cccagtgaat
     6061 ttgctaagct gagaacctgg tacttggcac aaggcagcgc tgtggataca gaagagccgt
     6121 ttatagcaca cacactacac cacacacgtg tgatacaaac aggagggaat gtgctgtatt
     6181 tttagacagt ggagcttgct catctgaaag tgggacatgg attagaaagt cccaggttca
     6241 aggcctcact ctgtcaacac ggaatagtca ctcacgtcag ctaaatgtaa ggacttcatt
     6301 acctgagagt aaggagccca gagcatcact gagggtaagc atggtggctt gactaggaaa
     6361 ggctaccgtg ggctaatata tttgatgctt ggtcattagg gtgtggagat acttgggagg
     6421 aattaggaag ggtggacttg gtggagattg tcgttagagg tgggctttga gctttcaaaa
     6481 gcccaggcca ggtccagtgt ctctctcttc ctgctgcctg tggagctgga ttgcaactct
     6541 caacttccct aacaccctgt ctgcctccag gccgccatgt tccccgccat gacaataaat
     6601 ggactaaacc tctgaagctg taagcaagcc tcagttaaat gctttctttt tgtttgtttg
     6661 ttgttttgtt ttgttttgtt ttgtttttca aaacagggtt tctctgtgta gccctggctg
     6721 tcctggaact cactctgtag accaggctgg cctcgaactc agaaatccgc ctgtacctgc
     6781 ctcccaagtg ccgggattaa aggcgtgcgc aatcacgcag gcttaaagcc ttgtcctggt
     6841 cgtggtatct cttcccaaca atgaatatac aaagtaacca ttaataaata cacagtttcc
     6901 tcctgcagat gcctactaaa acattcttct ccatctctct ttttgtgtgc agagtcagtc
     6961 ctttgtccta aactgaagcc ttctccagtg tccccaacag gacaggggtc ctctgacttg
     7021 actggggaag aaggcagcga ggctgaagcc acagatcaac ttcattaaac aacagtgaca
     7081 gagctcttct tctccttctc ttctttctct gagaacatgc ccattctctc tttctcttcc
     7141 agccccatta catggcaatt ctcctggtct caatcctcca ggccagtgaa ctagcccaag
     7201 ggaggcttca taataagaca aagcccaggg aaacagaaaa ctgtaggttc ttttgttcct
     7261 tgggagccac ccttgcgaga actgtgggtc cctaatcact aagctgtggg aattgctttt
     7321 ttctcctgta gcctggtcac ctggtgagct gtaaggaaca gctagctgtc aagtcctctg
     7381 cctgacacct agacctggca gctgccatgg acaccccagg taagcggtct tgtgcccaac
     7441 tcctgcatat gacctacgac tgccagagcc tgtgtgctga gtgcctgctg gggagtcaaa
     7501 gatacagatg aagcctgtgg tggcgcacgc ctttaatccc agcacttggg aggccagagg
     7561 caggcggatt tctgagttcg aggccagcct gatctacaaa gtgagttcca ggacagccag
     7621 ggctatacag agaaaccctg tctcaaaaac acacacacac acacacacac acacacacac
     7681 aaaaaaaaga tacagatgag aagcccaagg ggagcacaaa gtttcccagt cctaggtcac
     7741 agtggctctc cttcctcgct ttgtcttctg taaattccca ggagtcgcct cctcaatacc
     7801 cagcctgaaa aatggcagcc aggaggagaa agagggaagg agggagccaa attacaggca
     7861 gacagctcta ctcctctcaa ggtggcttcc tatcagctga gcctggggcc cagtccttga
     7921 ggaggccgcc tacccgtctc cagcctgagc cattcttcag tcttaaatgg ggccttcaat
     7981 atgggaatgg tcagttttta atcctaagtt tcaacagcac tggattaata aatgtctagg
     8041 agttcagtag ccctgagctg ggaggttggg agggagcttc tccatctaat cagagcataa
     8101 atgcttgcgc taatgagagg ttcacagact gagtggaggc accatggcaa gtgggccctg
     8161 ctggactcat gaatgcactg ggggtgtctc tgaggttgat gggtgtgatg gaactcattc
     8221 tgataggaag cctggcctca aactcacaga gacccccccg ctccagcctc tcaactgctg
     8281 gattaaaagc atgcactact atgccaggct cctttcatgt ttaaaattct tctgttggtt
     8341 ctatagatca ttcattcatt cattcattca ttcattcatt caaccaaaca ttcagagttt
     8401 ctaaagattt atttatttgt ttgtttgttt gtttgtttgt ttgtttgttt gtttattgta
     8461 tgtgtacacg gtctctgtct tcagacacac cagaagaggg catttgatcc cattacagat
     8521 gtttgtgagc gactgtgtgg ttgctgggat ttgaattcag gacatccagg agagcagtca
     8581 gtgctcttaa ctactgagcc aactctccag ctcttgttca ggacctgctc attctggccc
     8641 cgctccctgt agctgtacaa cccagaagag ggcatcagat ctcattatgg atggttgtga
     8701 cccaccatag gttgtgagcc accatgtggt tgctgggaaa ttaactcagg accttcagaa
     8761 gagcagtcag tgctcttaac tccccattaa ttgtttctaa agctggtgtt acttgccttt
     8821 atcattgttg tgactaataa gaatagattt attatgctta caatgttgtg tgatttggta
     8881 attccaggga tgcaaaaagg caggaggaaa agttatcagt tcacatcctg gcagacataa
     8941 agcagaaaca aagcagtgac aaaaacagaa aaacaagaca ttggccatca acccaagagg
     9001 agacacaccc tcagcgtacc agactccagc agggcccact tgccatggtg cctccactca
     9061 gtctgtgagc ctctcattag ctcaggcttt acgctctgat tagatggaga agccccctca
     9121 cagcccccag ataagggcta ctgaactcct aggtatttat taatccagtg ctgttgaaac
     9181 tcgggattaa aaactgacca ttcctgcgtt gaaggtcaca tttggggctg gagaatggct
     9241 tagtggttaa gggcacttgc tgctcctgca gaggacctgg gttcagttcc caacacccac
     9301 atcaggctcg cagtctgtaa actgcaacta catgggatcc aatggcctcc ataggcacca
     9361 ggcacacaca tggtgcgcac acataaacgc aggcaaacat tcatatatac aaaataaaaa
     9421 taaagagaaa gatgtgaatc cacattcaag agccaacatg aaaggcgcat aacgatacca
     9481 cgatatcaac aagtttgtac aaaaaagcag gctggcgccg gaaccgaagt tcctattccg
     9541 aagttcctat tctctagaaa gtataggaac ttcgaaccct ttcccacacc accctccaca
     9601 cttgccccaa acactgccaa ctatgtagga ggaaggggtt gggactaaca gaagaacccg
     9661 ttgtggggaa gctgttggga gggtcacttt atgttcttgc ccaaggtcag ttgggtggcc
     9721 tgcttctgat gaggtggtcc caaggtctgg ggtagaaggt gagagggaca ggccaccaag
     9781 gtcagccccc cccccctatc ccataggagc caggtccctc tcctggacag gaagactgaa
     9841 ggggagatgc cagagactca gtgaagcctg gggtacccta ttggagtcct tcaaggaaac
     9901 aaacttggcc tcaccaggcc tcagccttgg ctcctcctgg gaactctact gcccttggga
     9961 tccccttgta gttgtgggtt acataggaag ggggacggga ttccccttga ctggctagcc
    10021 tactcttttc ttcagtcttc tccatctcct ctcacctgtc tctcgaccct ttccctagga
    10081 tagacttgga aaaagataag gggagaaaac aaatgcaaac gaggccagaa agattttggc
    10141 tgggcattcc ttccgctagc ttttattggg atcccctagt ttgtgatagg ccttttagct
    10201 acatctgcca atccatctca ttttcacaca cacacacacc actttccttc tggtcagtgg
    10261 gcacatgtcc agcctcaagt ttatatcacc acccccaatg cccaacactt gtatggcctt
    10321 gggcgggtca tccccccccc cacccccagt atctgcaacc tcaagctagc ttgggtgcgt
    10381 tggttgtgga taagtagcta gactccagca accagtaacc tctgcccttt ctcctccatg
    10441 acaaccaggt cccaggtccc gaaaaccaaa gaagaagaac cctaacaaag aggacaagcg
    10501 gcctcgcaca gccttcactg ctgagcagct ccagaggctc aaggctgagt ttcagaccaa
    10561 caggtacctg acagagcagc ggcgccagag tctggcacag gagctcggta cccggaagat
    10621 ctggactcta gagaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc
    10681 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
    10741 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
    10801 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
    10861 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
    10921 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
    10981 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
    11041 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
    11101 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
    11161 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc
    11221 cacaaccatg gaagatcccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    11281 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    11341 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg
    11401 gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga
    11461 tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa
    11521 cgtgacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg
    11581 ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat
    11641 ttttgatggc gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg
    11701 ccaggacagt cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa
    11761 ccgcctcgcg gtgatggtgc tgcgctggag tgacggcagt tatctggaag atcaggatat
    11821 gtggcggatg agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat
    11881 cagcgatttc catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc
    11941 tgaagttcag atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca
    12001 gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg
    12061 tggtggttat gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag
    12121 cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct
    12181 gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct
    12241 gctgctgaac ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct
    12301 gcatggtcag gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa
    12361 caactttaac gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg
    12421 cgaccgctac ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc
    12481 aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg
    12541 aatggtgcag cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc
    12601 aggccacggc gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg
    12661 cccggtgcag tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat
    12721 gtacgcgcgc gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa
    12781 atggctttcg ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat
    12841 gggtaacagt cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt
    12901 acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg
    12961 caacccgtgg tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg
    13021 tatgaacggt ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca
    13081 ccagcagcag tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata
    13141 cctgttccgt catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc
    13201 gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact
    13261 gcctgaacta ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca
    13321 accgaacgcg accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct
    13381 ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac
    13441 cagcgaaatg gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc
    13501 aggctttctt tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga
    13561 tcagttcacc cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga
    13621 ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt
    13681 gttgcagtgc acggcagata cacttgctga tgcggtgctg attacgaccg ctcacgcgtg
    13741 gcagcatcag gggaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg
    13801 tcaaatggcg attaccgttg atgttgaagt ggcgagcgat acaccgcatc cggcgcggat
    13861 tggcctgaac tgccagctgg cgcaggtagc agagcgggta aactggctcg gattagggcc
    13921 gcaagaaaac tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt
    13981 gtcagacatg tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg
    14041 cgaattgaat tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta
    14101 cagtcaacag caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac
    14161 atggctgaat atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc
    14221 agtatcggcg gaattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca
    14281 aaaataataa taaccgggca ggggggatct aagctctaga taagtaatga tcataatcag
    14341 ccatatcaca tctgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    14401 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
    14461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    14521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ggaataactt
    14581 cgtatagcat acattatacg aagttatgtt tctagcttgg ctgcaggtcg tcgaaattct
    14641 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
    14701 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
    14761 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
    14821 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
    14881 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
    14941 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
    15001 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
    15061 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
    15121 cttcctcatc tccgggcctt tcgacctgca gcctgttgac aattaatcat cggcatagta
    15181 tatcggcata gtataatacg acaaggtgag gaactaaacc atgggatcgg ccattgaaca
    15241 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
    15301 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
    15361 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
    15421 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
    15481 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
    15541 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
    15601 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
    15661 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
    15721 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
    15781 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
    15841 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
    15901 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
    15961 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
    16021 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
    16081 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
    16141 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccccg
    16201 gatctaagct ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta
    16261 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    16321 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    16381 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    16441 aatgtatctt atcatgtctg gatccggggg taccgcgtcg agaagttcct attccgaagt
    16501 tcctattctc tagaaagtat aggaacttcg tcgagataac ttcgtatagc atacattata
    16561 cgaagttatg tcgagatatc tagacccagc tttcttgtac aaagtggttg atatctctat
    16621 agtcgcagta ggcggagaaa gccatcacta ggacttccct cttcctgggg ctgacggact
    16681 catgtcctca ttgttctcag ctgaaagaga cacaaatccc tgcaaagtac caggacacct
    16741 cccaggtgca ttagtctcct caggatgtca tcgaaaaggt cactgggcag gtggcttatc
    16801 aagaaaagat ctcttctctc agtgctctgg actcttgaca ctcacaacca aagtgcagca
    16861 ggttggccct ggacagcact ctcttcctgc ttggcagaga gttcccatgt cctgtctgtg
    16921 tgcctggaca gagggacggg ggcagagaaa gagaaaacag acaggaggaa gaaagagaag
    16981 gagggaaaag ggagggcagt ttcttcctgt ttctgtaagg gtgctaacag gagctcacac
    17041 acaccctcac ttaatttcaa tattatgtgt ccttaatgat tctgtctcta aatagagtga
    17101 ccagggttag agcttttaca aatgagttta aggacacact cagtccctat cactagtgac
    17161 accattaaaa tactccatgt gacaagggct cagacaagca attacccagc atgcccctga
    17221 cccattcaaa ctcttgtgca ctgtcctcta accatataca cttccttctc atctagacag
    17281 agaatgcttt gttgagagtg tggttaacta cctcctggac acatggagca cagaggacct
    17341 gcggctcctg ctgacagaag aagaaacctg gaaacacttt gtggcagaag ttgatttgtc
    17401 caggtcacct ccccgaggga tgcctgcagt accagggttc aggcctggtt ggcttgttct
    17461 gggcaggaat actctcccca gacagaccaa gtagttaggg ttgaaggtgt ctcttctcag
    17521 caaatatttc cctcaccata ttctctacca ggaaagagcc tctgcctggg gggagatggc
    17581 agcagccaca gggaacaaaa ctatccctgc ctttggcatt cctgtgacca caggaagtta
    17641 gttccttacc ttcttcttta tttggttact gttgactggc attgtgaaaa gcccaacata
    17701 tgtcccaaca tatgtccagt agtagacagc ctgcactcag taggtgttca tcaaaagtta
    17761 gcttttaaag tctctctctc tctctctctc tctctctctc tctctctctc tctctctctg
    17821 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tacatataca tgtgcatgtg
    17881 tggtttttgc atgtgggtga gtttatttta taagcagtct tcctgtatca gtcactctct
    17941 cctattacag ggaagaggaa gctgccttgc aggaatctct tgctgagatc tttgcagatc
    18001 ctgatggaga agatgaagat gagctccaaa atgaccttca ggacaagaac gagaggtact
    18061 cagccaaaga cccaatgaag cccttttctc tctagatagg aaggggggcg gggaaacatg
    18121 aatagaggac catgcacagg atattgaata ttcagggaca agctgattaa gtatgtgcct
    18181 gaacacctgc cacagggcct atttcaatga tgtcactagt gttagggact aagttgtatc
    18241 cctaagagat ggcgcaacgc aattaatgat aacttcgtat agcatacatt atacgaagtt
    18301 atggtctgag ctcgccatca gttcaaaccc atagcacagt caagtttata gtaaatcaag
    18361 gccaagagga cacgagatcc ctacagctgt gagccactaa gtgtagggtc tgggacctga
    18421 actcacttgg tctgcaagag aaccatgcat ctgaataaag aaactctctc tccagcccat
    18481 tacaataaca agtgccttta ctaacctgga actcatcact gtgaccagat tggctgacaa
    18541 gagagcatga ggaccctcct aaccttgcat ccccaagcag ggatgacagg cccaggccct
    18601 gaacctggct ttcctatgtg gcctctggaa gactgaactc gggtcctcct gtttgtatga
    18661 caaacactgc accaacttag ccacatcctc aggtcccttt taaggttttt tttttggggg
    18721 gggggggggc aggttggttg gttggttggt tggtttttcg aaatagggtt tctctgtgca
    18781 gtcctggctg tcctggaact cactctgtac accaggctgg cctcgaactc agaaatctgc
    18841 ctgcctccca gcactgcccg gctccttcaa aggctttaag cagaatcttc tgttgtcctc
    18901 aggacacagt gcctcatggt caaggtcaca tggagtgtct cacaccaaga aagcgcccat
    18961 gggaaagaac agttaatctg atagacgtta ccacaggcag gaaggcactg tccaggacgg
    19021 ctgctctaag acaaagacac aacaagtctg tcagttcctt cagctggaga ctcacagcag
    19081 atgagctgag tgacaggcag tagagagcag agagtagggg cattgtgagg ctgacctcat
    19141 gggactcctc ctgaggaaag ccaaggccaa acccaccaat gctggacatg ggaacctgat
    19201 ggaggttaag catgaaaggt cgcgtgctcg ccaacctgac ttaggattct gccaagtctg
    19261 tgcagggcaa gcacaaggtg cacaggaaga gcatagaaag ccaaggtcca tccatactgg
    19321 gaacagttta caggacttaa agttttctca aggaacagaa atgttgccaa cctaacttca
    19381 gtgtctagaa gtttgctggg ccttcatcaa atacaccgtt cccatgagtg actggggtct
    19441 ccctccctcc tggaagactg tgccttcagt gtccccttcc tctggggtca gaggtgactg
    19501 caaaatcccc accacacctc acctttctca ggctgagaat tctttaatcg aagggcttag
    19561 caacctgtgt gttttgaatt ctcaattccc tgttacttaa actcttacag aggcacagtg
    19621 atatgccttg gggataggag cccatgtgaa tagggacacc acacatgtga atggggattc
    19681 accacatgtg aatgtggaat ccactcatgt ttctttacct tcactcacaa ctggaaggaa
    19741 aatctatgcg ctgtggtttg agtggacaaa tcttagctgt gagttgtccc aacagctcag
    19801 gagtacacac cactgcagta caaaaaaaga cctcaagctt caaagagggt cctatgtacg
    19861 tttctctctt agggatgtgc aacttgcaga gtctcacagc acgaggctcc aggcagacat
    19921 ggacactcct ggccagcagg acaatgtagg gcagacagga catgtcccag cagctgtggc
    19981 caggagactg ctgttcttga ggaaagtgaa cccttcagtg aacactaacg ttccctaaat
    20041 aaaaggatta ttttgttttc tctaacttct ctctttttgt atagagttcc tagggagaag
    20101 tgttttggta gtgatgacat tgaccctgag ggaaaggctc ccttattaaa tgtctagcaa
    20161 taaaagggtg tgctggcaga cgccacaggc tgggcactga tgactgagtc catcagtgcc
    20221 cagccccctc atgatggctc aagactgttg aaattccagt tccaggagtc tgatgccttc
    20281 cgtcttctgc cctctacggg tctcaggaat gaatatggta cccagacaaa catgcagatg
    20341 aagcacccat acacataaga taaattaaaa ggacagggct agagaaattg ctcagtggtt
    20401 aagaacactg actgctcttc cagaggtcct gagttcaaat cccagcaacc acatggtggc
    20461 tcacagccat ctgtaatggg atcagatgcc ctcttctggt gtatctaaag accatgagag
    20521 tgtactcata cagataaaat aaataaaaac ataatcttaa aatattaaaa ggacaattat
    20581 agaaggtctt gaacttactg tgtccttagg aactaagaat ggtgtgattt gtcaagcatg
    20641 ctcgtcgcgg taagaaagag tttatacagc tgtaacattc tcctctgctt cacagtaaat
    20701 gatgatctgt ggtaacccca ggacagatct tactttacaa gctgtcttca tctcttagct
    20761 gtctctcctc ttacaggaaa gaggaagatt ccttgagtga agatcttggt gagagtgtag
    20821 ctgatactga tgtagaagat gaagatggtg atgcagaaga tgaagatagt gatacagaag
    20881 atgaagatag agatatagaa gatgaaaata gtaatagaga agatgaagat agtgatacag
    20941 aagatgaaga tagtgataca gaaggtgaag atagtaatac ggaagatgaa gaggagatcc
    21001 aaaatgatct gtggcacaag gaaaggttct tggatgctta tcctcaggtg aaactggagc
    21061 ttgaggagat catcaggaag ctccacgccc tggcagacaa ggttgacaag gtgcacaggg
    21121 actgcaccat ctcacgggtg gtggccagct ccagcagtgc tgtgtctgga gtcctgatca
    21181 tccttggtct gtctctggca cctgtgacag caggagtcag tctggcattg tcagccactg
    21241 gcttggggct gggggcagca gcggctgtga ctagtgtttc cacaagcatt gtggaaaagg
    21301 taagtgtggt gtctgctgaa gatgaagcca gcaagctggt gccaaccaac aaagacacaa
    21361 tgaagggcat gaaagaagtt ttggatcaga gtggtcccag acttctttct ttatctacag
    21421 acaccattca gagattgcaa ggcattcaga aatgcattaa tgccattccg ctggccaaag
    21481 ccaacactcg cctagcaaat aatgccaagc gtctcatgac cacagggaag atatctgccc
    21541 gaaacactaa acaggtgcag aaagcctttg gaggtaccgc tctggcaatg accaaaggag
    21601 cccgaatcat gggtgcagcc actgcaggtt tcttccttct gcaggatgtg atcagccttg
    21661 tggaagactc gaagcatttg catgaaggtg ctaagtcaca gactgctgca gagctgagga
    21721 agcaagctca ggacctggag cagaagttac aggagctcaa agaggttcat gacagcctga
    21781 ctaagtgacc tcttcatctt ctgtgcagct cagatgactg gggagggagt gtgctggaca
    21841 gagccatgaa cacatgggag cccctgactg tacccctcac agcagtgcct gctaaaggga
    21901 gagggcacat taaaatacag gaagccagaa cagagatggg acaatctcat gaccacttac
    21961 cagactccag ctcttagatc catcaacttc ccaattaaca gccaagctcc caagctgggg
    22021 agcgcatcca agtcctgact aagccacatg gctgctccta agctttgccc actgcactgc
    22081 tcccagctga ggctcaccaa cctcaccatc cactgagctg tcaatcttcc cactgctttg
    22141 tgattggtgg cacagagacc ttctcttttc ttcttctgcc tcattgtgtg tttttccaaa
    22201 tatttctcaa tcttacaatc agattctggt tgtgaacgtc atttcctgtt cttggttttg
    22261 ggtcacatgg gtgctgttgg tgaaaagctc aagactagaa agaggggatg ctgggaatac
    22321 cacctgtcca atcatcacaa gttcttggcc ttccatgcca ctgtggatgt taaggaaagg
    22381 agaggtgaca ttgtacgac
//